CN106478828A - A kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus - Google Patents
A kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus Download PDFInfo
- Publication number
- CN106478828A CN106478828A CN201610872848.2A CN201610872848A CN106478828A CN 106478828 A CN106478828 A CN 106478828A CN 201610872848 A CN201610872848 A CN 201610872848A CN 106478828 A CN106478828 A CN 106478828A
- Authority
- CN
- China
- Prior art keywords
- abelmoschus esculentus
- polysaccharide
- powder
- yield
- dryingaid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus.Methods described comprises the steps：1) beat powder after Abelmoschus esculentus are freeze-dried and obtain Abelmoschus esculentus dry powder；2) under ultrasonic wave added, described Abelmoschus esculentus dry powder is carried out hot water extraction, through collected after centrifugation supernatant, as Abelmoschus esculentus polysaccharide solution；3) carry out homogenizing after adding dryingaid and quick-dissolving agent in described Abelmoschus esculentus polysaccharide solution；4) the described Abelmoschus esculentus polysaccharide solution after described homogenizing is spray-dried, that is, obtain Abelmoschus esculentus polysaccharide powder.By the way of present invention powder processed after the Abelmoschus esculentus fresh fruit lyophilizing.Broken more complete after lyophilizing, the yield (i.e. yield) extracting polysaccharide powder than fresh fruit after directly crushing is high.By the way of the present invention extracts Abelmoschus esculentus polysaccharide using ultrasonic wave added hot water.Solve the problems, such as that polysaccharide extract rate is low, and process is briefly efficient, the yield of the polysaccharide powder extracted than conventional hot water is high.The present invention adds beta cyclodextrin as dryingaid.High compared to the yield without the mode gained polysaccharide powder being directly spray-dried under conditions of this dryingaid.
Description
Technical field
The present invention relates to a kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus, belong to feature
Food Engineering Development field.
Background technology
Abelmoschus esculentus (Abelmoschus esculentus (Linn.) Moench), another name Flos abelmoschi manihot, swordweed, are Malvaceae
Abelmoschuss annual herb plant, is widely distributed in the torrid zone, subtropical zones and warm Temperate Region in China.Abelmoschus esculentus are risen in non-
Continent, introduces China from early 1990s, all has cultivation, mesh in Asia, Middle East and southern US Zhu Zhou and other places now
The domestic Abelmoschus esculentus resource of front India is the abundantest.In recent years, China south and north various places also present the gesture of large area popularization,
Especially the widest with Taiwan area, wherein the Taining in Fujian Province, Jianning, by the plantation history in happy those counties for a century, in river
Pingxiang Shang Bu town one band of Xi Sheng was also planted for more than ten years.Abelmoschus esculentus fruit is that one kind has better nutritivity value and notable health care
The new type of health vegetable of function, its stem, leaf, flower, seed also have certain value of exploiting and utilizing simultaneously.Often eat its okra fruit
Pod can play shield stomach liver protection, lessen fatigue, health invigorating the effect of, be a kind of draft with important value of exploiting and utilizing
Plant resourceses.With the domestic increasingly increase to functional food demand, Abelmoschus esculentus are because of its special functional characteristic and good
Medical value is increasingly taken seriously.Although Abelmoschus esculentus gradually expand in the cultivated area of China, it is still in fresh vegetable and sells
Based on, deep processing level is low, added value is low state, do not form industrialization management, its due value could not be played.
Research shows, many containing be made up of poly galactose, galactan and arabinan and pectin in Abelmoschus esculentus
Sugared mixture.Polysaccharide is the important informational molecule of a biological internal class, has cell-surface signal identification, antigen-antibody anti-
Should, the biological agent of cell-tocell transmission and impression, and there are multiple biological activities, such as strengthen immunomodulating, antitumor, fall
Blood fat, blood sugar lowering, antioxidation, antiviral etc..Therefore exploitation Abelmoschus esculentus polysaccharide health-care product is significant, it will produce huge
Big Social benefit and economic benefit.
The product of rare Flos abelmoschi manihot polysaccharide at present on market, such health product has wide development space.And Flos abelmoschi manihot polysaccharide
Extract the hot water extraction mostly being Flos abelmoschi manihot fresh fruit, method is single, and polysaccharide yield is relatively low.
Content of the invention
It is an object of the invention to provide a kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus, this
Inventive method powder processed by the way of the fresh Flos abelmoschi manihot of lyophilization, it is to avoid the incomplete problem of Flos abelmoschi manihot disorganization；Side of the present invention
Method adopts the method that ultrasonic wave added hot water extracts, and solves the problems, such as that polysaccharide extract rate is low, and process is briefly efficient；Side of the present invention
Method adopts the mode of spraying can be with high efficiency grinding and yield is higher；The Abelmoschus esculentus polysaccharide powder of the inventive method preparation has higher
Oxidation resistance.
The method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus provided by the present invention, walks including following
Suddenly：
1) beat powder after Abelmoschus esculentus are freeze-dried and obtain Abelmoschus esculentus dry powder；
2) under ultrasonic wave added, described Abelmoschus esculentus dry powder is carried out hot water extraction, through collected after centrifugation supernatant, as yellow
Flos abelmoschi manihot polysaccharide solution；
3) carry out homogenizing after adding dryingaid and quick-dissolving agent in described Abelmoschus esculentus polysaccharide solution；
4) the described Abelmoschus esculentus polysaccharide solution after described homogenizing is spray-dried, that is, obtain Abelmoschus esculentus polysaccharide powder.
In above-mentioned method, step 1) in, the present invention carries out described freeze-drying process using after the section of Abelmoschus esculentus fresh fruit；
Compared with the mode of conventional hot air drying, described cryodesiccated every can save Huang to a great extent
The activity of functional component in Flos abelmoschi manihot；And carry out powder processed using the Abelmoschus esculentus after freeze-dried, the historrhexis of Abelmoschus esculentus can be made more
Completely, and then extract polysaccharide extraction yield relatively from fresh fruit from okra powder higher.
Described cryodesiccated condition is as follows：
Temperature is -40～-55 DEG C, and pressure is 8～12Pa, and the time is 24～36 hours, such as in the condition of -50 DEG C, 10Pa
Lower freezing 30 hours；
The mesh number of described Abelmoschus esculentus dry powder is 30～40 mesh.
In above-mentioned method, step 2) in, described ultrasonic power can be 400～700W, concretely 400～600W,
400W or 600W；
The condition that described hot water extracts is as follows：
Described Abelmoschus esculentus dry powder can be 1g with the proportioning of water：20～60mL, concretely 1g：30～40mL, 1g：30mL or
1g：40mL；
PH value can be 7.5～9.0, concretely 8.0～8.5,8.0 or 8.5；
Temperature can be 40 DEG C～60 DEG C, concretely 40 DEG C or 60 DEG C；
Time can be 15～40min, concretely 20～30min, 20min or 30min.
In above-mentioned method, methods described includes repeat step 2) described in hot water extract the step of 1～3 time.
In above-mentioned method, step 2) in, described centrifugation carries out 5～10min under 8000rpm, such as 7min.
In above-mentioned method, step 3) in, described dryingaid can be beta-schardinger dextrin-；
Described dryingaid decreases wall cling phenomenon in described spray-drying process for the polysaccharide, and the moisture reducing polysaccharide powder contains
Amount, extends the shelf life；
Described quick-dissolving agent can be lecithin.
Described quick-dissolving agent is effectively improved dispersibility and the water solublity of Flos abelmoschi manihot polysaccharide powder.
In above-mentioned method, the addition of described dryingaid can be the total solids content of described Abelmoschus esculentus polysaccharide solution
10～30%, concretely 20～30%, 20% or 30%；
The addition of described quick-dissolving agent can be the 2～3% of the total solids content of described Abelmoschus esculentus polysaccharide solution, specifically
Can be 2%.
In above-mentioned method, step 3) in, the pressure of described homogenizing can be 15～27MPa, concretely 18～25MPa,
18MPa or 25MPa, number of times can be 4～7 times, concretely 4～6 times, 4 times or 6 times.
In above-mentioned method, step 4) in, the condition of described spray drying is as follows：
Inlet temperature can be 150～200 DEG C, concretely 160～170 DEG C, 160 DEG C or 170 DEG C, and leaving air temp can be 80
～100 DEG C, concretely 90～100 DEG C, 90 DEG C or 100 DEG C.
The Abelmoschus esculentus polysaccharide powder that said method of the present invention obtains falls within protection scope of the present invention.
The present invention has the advantages that：
(1) by the way of present invention powder processed after the Abelmoschus esculentus fresh fruit lyophilizing.
Broken more complete after lyophilizing, the yield (i.e. yield) extracting polysaccharide powder than fresh fruit after directly crushing is high, and polysaccharide powder obtains
Up to 2.2 times of rate.
(2) present invention is by the way of ultrasonic wave added hot water extracts Abelmoschus esculentus polysaccharide.
Ultrasonic wave added hot water extracts and solves the problems, such as that polysaccharide extract rate is low, and process is briefly efficient, carries than conventional hot water
The yield of the polysaccharide powder taking is high, up to 1.65 times of polysaccharide powder yield.
(3) present invention adds beta-schardinger dextrin-as dryingaid.
It is high compared to the yield without the mode gained polysaccharide powder being directly spray-dried under conditions of this dryingaid,
Up to 1.93 times of polysaccharide powder yield.
Specific embodiment
Experimental technique used in following embodiments if no special instructions, is conventional method.
Material used, reagent etc. in following embodiments, if no special instructions, all commercially obtain.
The Abelmoschus esculentus dry powder that following embodiments are adopted is prepared in accordance with the following steps：
Carry out lyophilization after Abelmoschus esculentus fresh fruit is cut into slices：Temperature is -50 DEG C, and pressure is 10Pa, and cooling time is little for 30
When；Abelmoschus esculentus section after lyophilizing is carried out beating and after powder, crosses 40 mesh sieves, obtain Abelmoschus esculentus dry powder.
In following embodiments, total economic value using total antioxidant capacity (T-AOC) detection kit, (colorimetry, build by Nanjing
Become Bioengineering Research Institute) it is measured.
Embodiment 1,
100g Abelmoschus esculentus dry powder, add water 3000mL, adjusts pH to 8.0, at 40 DEG C, 400W supersound process 20min.Extract
8000rpm centrifugation 7min after end, takes supernatant to be Abelmoschus esculentus polysaccharide solution, repeats to extract 3 times.To the many sucrose solution of Abelmoschus esculentus
Add beta-schardinger dextrin-and lecithin, beta-schardinger dextrin-addition is total solids content in Abelmoschus esculentus polysaccharide solution in solution
20%, the addition of lecithin is 2% of total solids content in Abelmoschus esculentus polysaccharide solution.Add dryingaid and quick-dissolving agent it
After carry out homogenizing, homogenization pressure be 18MPa, homogenisation cycle be 4 times.Solution after homogenizing is spray-dried, and controls into pathogenic wind-warm
Spend for 160 DEG C, leaving air temp is 90 DEG C, obtains Abelmoschus esculentus polysaccharide powder 18.75g.
The yield of the present embodiment Abelmoschus esculentus polysaccharide powder is 18.75%.
Water content≤6% of Abelmoschus esculentus polysaccharide powder manufactured in the present embodiment.
In Abelmoschus esculentus polysaccharide powder manufactured in the present embodiment, polyoses content is 84.13%.
After measured, the total economic value of the Abelmoschus esculentus polysaccharide xeraphium manufactured in the present embodiment of 10mg/mL is equivalent to 89.5 μ
The Trolox of g/mL.
Embodiment 2,
100g Abelmoschus esculentus dry powder, add water 4000mL, adjusts pH to 8.5, at 60 DEG C, 600W supersound process 30min.Extract
8000rpm centrifugation 7min after end, takes supernatant to be Abelmoschus esculentus polysaccharide solution, repeats to extract 3 times.To the many sucrose solution of Abelmoschus esculentus
Add beta-schardinger dextrin-and lecithin, beta-schardinger dextrin-addition is total solids content in Abelmoschus esculentus polysaccharide solution in solution
30%, the addition of lecithin is 2% of total solids content in Abelmoschus esculentus polysaccharide solution.Add dryingaid and quick-dissolving agent it
After carry out homogenizing, homogenization pressure is 25MPa, and homogenisation cycle goes as 6 times.Solution after homogenizing is spray-dried, and controls air intake
Temperature is 170 DEG C, and leaving air temp is 100 DEG C, obtains Abelmoschus esculentus polysaccharide powder 21.33g.
The yield of the present embodiment Abelmoschus esculentus polysaccharide powder is 21.33%.
Water content≤5% of Abelmoschus esculentus polysaccharide powder manufactured in the present embodiment.
In Abelmoschus esculentus polysaccharide powder manufactured in the present embodiment, polyoses content is 86.06%.
After measured, the total economic value of the Abelmoschus esculentus polysaccharide powder manufactured in the present embodiment of 10mg/mL is equivalent to 80.1 μ g/mL
Trolox.
Comparative example 1,
Identical with the process step of embodiment 1, difference is：Slurrying is crushed using Abelmoschus esculentus fresh fruit.
This comparative example obtains Abelmoschus esculentus polysaccharide powder 9.82g.
The yield of this comparative example Abelmoschus esculentus polysaccharide powder is 9.82%, adopts far below the present invention and makes after Abelmoschus esculentus fresh fruit lyophilizing
The yield of powder, in the embodiment of the present invention 2, the yield of polysaccharide powder is 2.2 times of the yield of this comparative example polysaccharide powder.
Comparative example 2,
Identical with the process step of embodiment 2, difference is：Under ultrasonic wave added, do not carry out hot water extraction.
This comparative example obtains Abelmoschus esculentus polysaccharide powder 12.93g.
The yield of this comparative example Abelmoschus esculentus polysaccharide powder is 12.93%, adopts the many of ultrasonic wave added hot water far below the present invention
The yield of Icing Sugar, in the embodiment of the present invention 2, the yield of polysaccharide powder is 1.65 times of the yield of this comparative example polysaccharide powder.
Comparative example 3,
Identical with the process step of embodiment 2, difference is：It is added lecithin in Abelmoschus esculentus polysaccharide solution
Fat, without beta-schardinger dextrin-and lecithin.
This comparative example obtains Abelmoschus esculentus polysaccharide powder 11.05g.
The yield of this comparative example Abelmoschus esculentus polysaccharide powder is 11.05%, far below the present invention using water-soluble to Abelmoschus esculentus polysaccharide
Carry out after adding dryingaid beta-schardinger dextrin-in liquid being spray-dried the yield of gained polysaccharide powder, polysaccharide powder in the embodiment of the present invention 2
Yield is 1.93 times of the yield of this comparative example polysaccharide powder.
Claims (10)
1. a kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus, comprises the steps：
1) beat powder after Abelmoschus esculentus are freeze-dried and obtain Abelmoschus esculentus dry powder；
2) under ultrasonic wave added, described Abelmoschus esculentus dry powder is carried out hot water extraction, through collected after centrifugation supernatant, as Abelmoschus esculentus
Polysaccharide solution；
3) carry out homogenizing after adding dryingaid and quick-dissolving agent in described Abelmoschus esculentus polysaccharide solution；
4) the described Abelmoschus esculentus polysaccharide solution after described homogenizing is spray-dried, that is, obtain Abelmoschus esculentus polysaccharide powder.
2. method according to claim 1 it is characterised in that：Step 1) in, carry out described cold after described Abelmoschus esculentus section
Freeze dried；
Described cryodesiccated condition is as follows：
Temperature is -40～-55 DEG C, and pressure is 8～12Pa, and the time is 24～36 hours；
The mesh number of described Abelmoschus esculentus dry powder is 30～40 mesh.
3. method according to claim 1 and 2 it is characterised in that：Step 2) in, described ultrasonic power be 400～
700W；
The condition that described hot water extracts is as follows：
Described Abelmoschus esculentus dry powder is 1g with the proportioning of water：20～60mL；
PH value is 7.5～9.0；
Temperature is 40 DEG C～60 DEG C；
Time is 15～40min.
4. the method according to any one of claim 1-3 it is characterised in that：Methods described includes repeat step 2) in institute
State the step that hot water extracts 1～3 time.
5. the method according to any one of claim 1-4 it is characterised in that：Step 2) in, described centrifugation is in 8000rpm
Under carry out 5～10min.
6. the method according to any one of claim 1-5 it is characterised in that：Step 3) in, described dryingaid is β-ring
Dextrin；
Described quick-dissolving agent is lecithin.
7. method according to claim 6 it is characterised in that：The addition of described dryingaid is the many sucrose solution of described Abelmoschus esculentus
The 10～30% of the total solids content of solution；
The addition of described quick-dissolving agent is the 2～3% of the total solids content of described Abelmoschus esculentus polysaccharide solution.
8. the method according to any one of claim 1-7 it is characterised in that：Step 3) in, the pressure of described homogenizing is
15～27MPa, number of times is 4～7 times.
9. the method according to any one of claim 1-8 it is characterised in that：Step 4) in, the bar of described spray drying
Part is as follows：
Inlet temperature is 150～200 DEG C, and leaving air temp is 80～100 DEG C.
10. the Abelmoschus esculentus polysaccharide powder of method preparation any one of claim 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610872848.2A CN106478828A (en) | 2016-09-30 | 2016-09-30 | A kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610872848.2A CN106478828A (en) | 2016-09-30 | 2016-09-30 | A kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106478828A true CN106478828A (en) | 2017-03-08 |
Family
ID=58269134
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610872848.2A Pending CN106478828A (en) | 2016-09-30 | 2016-09-30 | A kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106478828A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109053925A (en) * | 2018-08-13 | 2018-12-21 | 合肥工业大学 | A kind of separating and extracting process and application thereof of alkalinity okra polysaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791363A (en) * | 2010-01-11 | 2010-08-04 | 杨昌燕 | Broad spectrum anticancer vegetable drug, preparation method and application thereof |
| CN103860631A (en) * | 2014-03-04 | 2014-06-18 | 内蒙古普凡生生物科技有限公司 | Method for preparing liquorice active substances |
| CN103919077A (en) * | 2014-05-09 | 2014-07-16 | 新疆源森农业开发有限公司 | Seed-melon full powder and preparing method thereof |
-
2016
- 2016-09-30 CN CN201610872848.2A patent/CN106478828A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791363A (en) * | 2010-01-11 | 2010-08-04 | 杨昌燕 | Broad spectrum anticancer vegetable drug, preparation method and application thereof |
| CN103860631A (en) * | 2014-03-04 | 2014-06-18 | 内蒙古普凡生生物科技有限公司 | Method for preparing liquorice active substances |
| CN103919077A (en) * | 2014-05-09 | 2014-07-16 | 新疆源森农业开发有限公司 | Seed-melon full powder and preparing method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 任丹丹 等: "响应面法优化黄秋葵多糖超声提取工艺", 《食品科学》 * |
| 何东平 等: "《粮油食品》", 30 June 2014, 中国轻工业出版社 * |
| 包海鹰: "《中药学》", 30 June 2008, 中国农业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109053925A (en) * | 2018-08-13 | 2018-12-21 | 合肥工业大学 | A kind of separating and extracting process and application thereof of alkalinity okra polysaccharide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101999609B (en) | Method for making potato-fruit recombinant mixed crispy chips | |
| CN106699917B (en) | A kind of method of ultrasound assisted extraction okra polyoses extract and pectin extraction object | |
| CN107099375B (en) | Preparation method of cyperus esculentus oil and cyperus esculentus powder | |
| ES2594811T3 (en) | Method for manufacturing bioproducts with a modified sugar profile | |
| CN103333268A (en) | Method for preparing lycium ruthenicum polysaccharide | |
| CN102391549A (en) | Preparation method of abelmoschs esculentus water-soluble polysaccharide spray-dried powder | |
| CN101385829B (en) | Fresh gastrodia tuber full powder and preparation technique thereof | |
| CN103355672A (en) | Preparation method of bamboo shoot dietary fiber submicron powder electuary | |
| CN103535410A (en) | Body-nourishing healthcare biscuit and preparation method thereof | |
| CN102715318A (en) | Granulated tea of kudzu and method for preparing same | |
| CN107637768A (en) | A kind of processing method of red bean coixlacrymajobi powder | |
| CN104945154A (en) | Nutritionally-balanced oyster mushroom culture medium prepared from melaleuca alternifolia residues and preparation method | |
| CN104814476B (en) | The preparation method that convenient and instant nurses one's health shrimp | |
| CN103070431B (en) | Kelp extract iodine tablet and preparation method thereof | |
| CN106262105A (en) | A kind of processing method of vacuum lyophilization Fructus Cucurbitae moschatae powder | |
| CN104231104A (en) | Extraction method of polysaccharides in coriolus versicolor | |
| CN102860354A (en) | Quick-frozen fresh-kept gastrodia elata blume and technology for preparing same | |
| CN106478828A (en) | A kind of method having anti-oxidation function polysaccharide powder in ultrasound assisted extraction Abelmoschus esculentus | |
| CN104055022A (en) | Teenager brain strengthening flour and preparation method thereof | |
| CN102894241A (en) | Processing technology for efficiently increasing gastrodin content in gastrodia elata blume | |
| CN105520093A (en) | Preparation method of ultra-micro nutritional garlic whole powder | |
| CN105961644A (en) | Cold pressed sesame oil with efficacy of reducing blood fat and preparation method thereof | |
| CN106690167A (en) | Method for processing wash-free lycium ruthenicum lyophilized product | |
| CN105011240A (en) | Method for producing walnut powder and walnut-flavored sauce, rich in natto kinase, by fermentation method | |
| CN104770744A (en) | Bamboo sprout amino acid preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170308 |
|
| RJ01 | Rejection of invention patent application after publication |