CN106475568A - The preparation of Au nanoparticle - Google Patents
The preparation of Au nanoparticle Download PDFInfo
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- CN106475568A CN106475568A CN201510535689.2A CN201510535689A CN106475568A CN 106475568 A CN106475568 A CN 106475568A CN 201510535689 A CN201510535689 A CN 201510535689A CN 106475568 A CN106475568 A CN 106475568A
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Abstract
The present invention relates to the preparation of Au nanoparticle, concrete grammar is as follows：Prepare 0.01% three chloride hydrate gold first（HAuCl4·3H2O）Solution and 1% trisodium citrate（C6H5Na3O7·3H2O）Solution, and use 0.22μThe porous membrane of m is filtered to remove impurity respectively, then by 1.0mL trisodium citrate（C6H5Na3O7·3H2O）Solution is added to three chloride hydrate gold of 100mL boiling（HAuCl4·3H2O）In solution, continue insulated and stirred 20min, naturally cool to room temperature.By the gold size preparing solution be stored in 4 DEG C standby.
Description
Technical field
The present invention relates to the preparation of Au nanoparticle, belong to analysis applied chemistry field.
Background technology
Extensive concern is caused based on the electrochemical gene detection method of nanoparticle.There are gold colloidal, semiconductor-quantum-point tracer, ferrum/billon nanoparticle, copper/billon nanoparticle and Nano silver grain through commonly used nanoparticle in said method.These nanoparticles are to provide effective method by the surface DNA identification of electrochemical sensing and the amplification of electrochemical response.The Electrochemical Stripping that most of method often relies on high-sensitive metal tracer is sensed or is measured.Stripping voltammetry is a kind of method of very effective detection trace metal.The high sensitivity of the method ought give the credit to " built-in "（Built-in）Pre-enrichment step, in this step, metal target assemble（Deposition）To on working electrode.Therefore, compared with detecting the Pulse Voltammetry technology that DNA hybridization uses in early time, low 3 ~ 4 orders of magnitude of test limit.
Content of the invention
The present invention provides the preparation of Au nanoparticle, and concrete grammar is as follows：Prepare the three chloride hydrate gold of 0.01 % first（HAuCl4·3H2O）Solution and the trisodium citrate of 1 %（C6H5Na3O7·3H2O）Solution, and use 0.22μThe porous membrane of m is filtered to remove impurity respectively, then by 1.0 mL trisodium citrates（C6H5Na3O7·3H2O）Solution is added to three chloride hydrate gold of 100 mL boilings（HAuCl4·3H2O）In solution, continue insulated and stirred 20 min, naturally cool to room temperature.By the gold size preparing solution be stored in 4 °C standby.
Further, the preparation of described Au nanoparticle, reaction temperature is 100 degree.Mixing speed turns/min for 200-300.
The invention has the beneficial effects as follows：Gold size nanoparticle is introduced space by crossover process, and subsequently the deposition of silver can produce the signal of telecommunication being available for measuring.The signal of telecommunication that hybridization produces, and between electrode gap associated therewith resistance change, make this method have very high sensitivity, detection is limited to 0.5 pM.
Specific embodiment
Hereinafter the principle and feature of the present invention is described, example is served only for explaining the present invention, is not intended to limit the scope of the present invention.
Embodiment 1
Prepare 0.01 first
The three chloride hydrate gold of %（HAuCl4·3H2O）Solution and the trisodium citrate of 1 %（C6H5Na3O7·3H2O）Solution, and use 0.22μThe porous membrane of m is filtered to remove impurity respectively, then by 1.0 mL trisodium citrates（C6H5Na3O7·3H2O）Solution is added to three chloride hydrate gold of 100 mL boilings（HAuCl4·3H2O）In solution, continue insulated and stirred 20 min, naturally cool to room temperature.By the gold size preparing solution be stored in 4 °C standby.
Embodiment 2
Prepare 0.02 first
The three chloride hydrate gold of %（HAuCl4·3H2O）Solution and the trisodium citrate of 1 %（C6H5Na3O7·3H2O）Solution, and use 0.22μThe porous membrane of m is filtered to remove impurity respectively, then by 2.0 mL trisodium citrates（C6H5Na3O7·3H2O）Solution is added to three chloride hydrate gold of 100 mL boilings（HAuCl4·3H2O）In solution, continue insulated and stirred 20 min, naturally cool to room temperature.By the gold size preparing solution be stored in 4 °C standby.
Embodiment 3
Prepare 0.015 first
The three chloride hydrate gold of %（HAuCl4·3H2O）Solution and the trisodium citrate of 1 %（C6H5Na3O7·3H2O）Solution, and use 0.22μThe porous membrane of m is filtered to remove impurity respectively, then by 1.50 mL trisodium citrates（C6H5Na3O7·3H2O）Solution is added to three chloride hydrate gold of 100 mL boilings（HAuCl4·3H2O）In solution, continue insulated and stirred 20 min, naturally cool to room temperature.By the gold size preparing solution be stored in 4 °C standby.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any modification, equivalent substitution and improvement made etc., should be included within the scope of the present invention.
Claims (3)
- The preparation of 1.Au nanoparticle, concrete grammar is as follows：Prepare the three chloride hydrate gold of 0.01 % first（HAuCl4·3H2O）Solution and the trisodium citrate of 1 %（C6H5Na3O7·3H2O）Solution, and use 0.22μThe porous membrane of m is filtered to remove impurity respectively, then by 1.0 mL trisodium citrates（C6H5Na3O7·3H2O）Solution is added to three chloride hydrate gold of 100 mL boilings（HAuCl4·3H2O）In solution, continue insulated and stirred 20 min, naturally cool to room temperature, by the gold size preparing solution be stored in 4 °C standby.
- 2. the preparation of Au nanoparticle described in claim 1, reaction temperature is 100 degree.
- 3. the preparation of Au nanoparticle described in claim 1, mixing speed turns/min for 200-300.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510535689.2A CN106475568A (en) | 2015-08-28 | 2015-08-28 | The preparation of Au nanoparticle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510535689.2A CN106475568A (en) | 2015-08-28 | 2015-08-28 | The preparation of Au nanoparticle |
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| CN106475568A true CN106475568A (en) | 2017-03-08 |
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| CN201510535689.2A Pending CN106475568A (en) | 2015-08-28 | 2015-08-28 | The preparation of Au nanoparticle |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107498065A (en) * | 2017-09-06 | 2017-12-22 | 烟台智本知识产权运营管理有限公司 | The synthesis of gold size nano-particle |
| CN111020006A (en) * | 2019-11-22 | 2020-04-17 | 东南大学 | Electrochemical luminescence sensor system for measuring adenosine triphosphate, and preparation method and application thereof |
| CN112191259A (en) * | 2020-10-22 | 2021-01-08 | 黄河科技学院 | MXene/Au photocatalytic nitrogen fixation material, and preparation method and application thereof |
-
2015
- 2015-08-28 CN CN201510535689.2A patent/CN106475568A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107498065A (en) * | 2017-09-06 | 2017-12-22 | 烟台智本知识产权运营管理有限公司 | The synthesis of gold size nano-particle |
| CN111020006A (en) * | 2019-11-22 | 2020-04-17 | 东南大学 | Electrochemical luminescence sensor system for measuring adenosine triphosphate, and preparation method and application thereof |
| CN111020006B (en) * | 2019-11-22 | 2023-04-07 | 东南大学 | Electrochemical luminescence sensor system for measuring adenosine triphosphate, and preparation method and application thereof |
| CN112191259A (en) * | 2020-10-22 | 2021-01-08 | 黄河科技学院 | MXene/Au photocatalytic nitrogen fixation material, and preparation method and application thereof |
| CN112191259B (en) * | 2020-10-22 | 2022-09-16 | 黄河科技学院 | MXene/Au photocatalytic nitrogen fixation material, and preparation method and application thereof |
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Application publication date: 20170308 |
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| WD01 | Invention patent application deemed withdrawn after publication |