CN106465830B - Daily dietary fiber solid beverage and preparation method thereof - Google Patents
Daily dietary fiber solid beverage and preparation method thereof Download PDFInfo
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- CN106465830B CN106465830B CN201510501940.3A CN201510501940A CN106465830B CN 106465830 B CN106465830 B CN 106465830B CN 201510501940 A CN201510501940 A CN 201510501940A CN 106465830 B CN106465830 B CN 106465830B
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Non-Alcoholic Beverages (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a daily dietary fiber solid beverage and a preparation method thereof. The daily dietary fiber solid beverage comprises the following raw materials: insoluble fiber powder, orange peel pectin, konjaku flour, resistant dextrin and auxiliary materials. The preparation method comprises the following steps: stirring and mixing insoluble fiber powder, orange peel pectin, rhizoma Amorphophalli powder, resistant dextrin and adjuvants, and sterilizing. The daily dietary fiber solid beverage has the beneficial effects of promoting intestinal peristalsis, being beneficial to discharge harmful substances in the digestive tract and preventing constipation and colon cancer.
Description
Technical Field
The invention relates to the field of food, in particular to a daily dietary fiber solid beverage and a preparation method thereof.
Background
Dietary fiber refers to a food nutrient which is not easily digested by human body, and is composed of carbohydrate, mainly from plant cell wall. It is indispensable to human healthy diet and plays an important role in maintaining the health of the digestive system. The dietary fiber has strong water absorption and viscosity effects, increases food volume in stomach and intestinal tract, and enhances satiety, and can adsorb intestinal cholesterol and harmful substances for excretion. In addition, it can promote gastrointestinal peristalsis, reduce the absorption of glucose and cholesterol by digestive tract, and has good prevention and treatment effects on constipation, colon cancer, cardiovascular diseases, diabetes, hypertension and other diseases.
The dietary fiber can be divided into two types of insoluble dietary fiber and water-soluble dietary fiber according to the difference of water solubility, and plant food usually contains the two types of dietary fiber, but the amounts of the two types of dietary fiber are different. The insoluble dietary fiber is not involved in the circulation of body fluid and blood of human body, and can reduce the retention time of excrement in intestinal tract and increase the volume of feces, thereby playing the role of relaxing bowel. The water-soluble dietary fiber can be dissolved in water, participates in the circulation of human blood and body fluid, can reduce postprandial blood sugar and blood insulin rising reaction, and plays an important role in preventing and treating various chronic diseases.
The probiotics refer to a class of active microorganisms beneficial to a host, mainly exist in the intestinal tract and the reproductive system of a human body, and have very positive effects on maintaining the balance of the intestinal flora of the human body and the health of the human body. The probiotics mainly comprise three categories of lactobacillus, bifidobacterium and gram-positive coccus. The reasonable supplement of probiotics in the healthy diet can play roles in promoting intestinal health, improving human immunity, reducing cholesterol, helping the absorption of nutrient substances and the like.
The Chinese Nutrition society recommends that each person should take 30.2 g of dietary fiber every day in the reference intake of dietary nutrients of Chinese residents published in 2000, and the survey shows that the daily intake of dietary fiber of each person in China is only 13.3 g. The reasonable supplement of dietary fiber has very positive effects on reducing the cholesterol content in blood and preventing cardiovascular and cerebrovascular diseases, colon cancer, rectal cancer and the like. At present, the consciousness of residents in China on the intake of dietary fiber supplement is still relatively thin, and dietary fiber products in the market do not have formula products for supplementing different requirements of human bodies day and night.
Disclosure of Invention
The invention aims to solve the technical problem that dietary fiber products aiming at different requirements of human bodies day and night do not exist in the prior art, and provides a daily dietary fiber solid beverage and a preparation method thereof. The dietary fiber solid beverage for daily use has the beneficial effects of promoting intestinal tract peristalsis, facilitating discharge of harmful substances in the digestive tract, and preventing constipation and colon cancer, and is an ideal dietary fiber supplement for human bodies. The preparation process has simple route and convenient operation.
The invention solves the technical problems through the following technical scheme:
The invention provides a daily dietary fiber solid beverage, which comprises the following raw materials: insoluble fiber powder, orange peel pectin, konjaku flour, resistant dextrin and auxiliary materials.
Wherein, the raw materials of the daily dietary fiber solid beverage preferably comprise the following components: insoluble fiber powder, orange peel pectin, konjaku flour, resistant dextrin and auxiliary materials.
Wherein, preferably, the raw materials of the daily dietary fiber solid beverage comprise the following components in percentage by mass: 1-90% of insoluble fiber powder, 0.1-50% of orange peel pectin, 1-50% of konjac flour, 1-80% of resistant dextrin and 1-50% of auxiliary materials; the percentage is the mass percentage relative to the total mass of the raw materials. Further preferably, the raw materials of the daily dietary fiber solid beverage comprise the following components in percentage by mass: 20-70% of insoluble fiber powder, 0.1-20% of orange peel pectin, 1-30% of konjac flour, 1-30% of resistant dextrin and 1-30% of auxiliary materials; the percentage is the mass percentage relative to the total mass of the raw materials.
Wherein, the insoluble fiber powder preferably comprises the following components: 20 to 80 percent of wheat fiber powder, 10 to 40 percent of soybean fiber powder, 1 to 20 percent of sweet potato fiber powder and 1 to 20 percent of corn fiber powder, wherein the percentage is mass percentage.
In the invention, the auxiliary material is preferably one or more of edible essence, acidity agent and thickening agent.
Wherein the edible essence is preferably one or more of apple essence, lemon essence and orange juice essence; the thickening agent is preferably one or more of guar gum, pectin, microcrystalline cellulose, sodium alginate and sodium carboxymethyl cellulose; the sour agent is preferably one or more of citric acid, malic acid and lactic acid.
The auxiliary material preferably further comprises a sweetener, and the sweetener is preferably one or more of sucralose, aspartame, sodium cyclamate and neotame; the amount of the sweetener is the amount which is conventional in the art, and the sweetener should be added according to the national standards for food additives.
The daily dietary fiber solid beverage can be prepared into other types of foods together with other ingredients acceptable in the dietetics.
In order to obtain better mouthfeel and functional effect, the daily dietary fiber solid beverage is recommended to be taken by 8-10 g per day, and the preferable dilution multiple is 1: the composition is taken after 30-35 days.
The invention also provides a preparation method of the daily dietary fiber solid beverage, which comprises the following steps: stirring and mixing insoluble fiber powder, orange peel pectin, rhizoma Amorphophalli powder, resistant dextrin and adjuvants, and sterilizing.
The sterilization is preferably microwave sterilization, the sterilization temperature is preferably 75-80 ℃, and the sterilization time is preferably 2-3 min.
Wherein, the stirring and mixing time is preferably 3-5 min.
The invention also provides an overnight dietary fiber solid beverage which comprises the following raw materials: resistant dextrin, L-arabinose, xylo-oligosaccharide, isomaltose oligosaccharide, orange peel pectin, bifidobacterium animalis, lactobacillus acidophilus and auxiliary materials.
Wherein, the raw materials of the night dietary fiber solid beverage preferably comprise the following components: resistant dextrin, L-arabinose, xylo-oligosaccharide, isomaltose oligosaccharide, orange peel pectin, bifidobacterium animalis, lactobacillus acidophilus and auxiliary materials.
wherein, preferably, the raw materials of the night dietary fiber solid beverage comprise the following components in percentage by mass: 1 to 80 percent of resistant dextrin, 0.1 to 50 percent of L-arabinose, 1 to 50 percent of xylo-oligosaccharide, 1 to 50 percent of isomaltose hypgather, 0.1 to 50 percent of orange peel pectin, 0.1 to 30 percent of animal bifidobacterium, 0.1 to 30 percent of lactobacillus acidophilus and 1 to 50 percent of auxiliary materials. The percentage is the mass percentage relative to the total mass of the raw materials. Further preferably, the raw materials of the night dietary fiber solid beverage comprise the following components in percentage by mass: 10 to 80 percent of resistant dextrin, 0.1 to 20 percent of L-arabinose, 1 to 30 percent of xylo-oligosaccharide, 2 to 10 percent of isomalto-oligosaccharide, 0.1 to 10 percent of orange peel pectin, 0.1 to 10 percent of animal bifidobacterium, 0.1 to 10 percent of lactobacillus acidophilus and 1 to 30 percent of auxiliary materials.
In the invention, the auxiliary material is preferably one or more of edible essence, acidity agent and thickening agent.
Wherein the edible essence is preferably one or more of apple essence, lemon essence and orange juice essence; the thickening agent is preferably one or more of guar gum, pectin, microcrystalline cellulose, sodium alginate and sodium carboxymethyl cellulose; the sour agent is preferably one or more of citric acid, malic acid and lactic acid.
The auxiliary material preferably further comprises a sweetener, and the sweetener is preferably one or more of sucralose, aspartame, sodium cyclamate and neotame; the amount of the sweetener is the amount which is conventional in the art, and the sweetener should be added according to the national standards for food additives.
The night dietary fiber solid beverage can be prepared into other types of foods together with other ingredients acceptable in the dietetics.
In order to obtain better mouthfeel and functional effect, the night dietary fiber solid beverage is recommended to be taken by 8-10 g every night, and the preferable dilution times are 1: the composition is taken after 30-35 days.
The invention also provides a preparation method of the night dietary fiber solid beverage, which comprises the following steps: stirring and mixing the resistant dextrin, the L-arabinose, the xylo-oligosaccharide, the isomaltooligosaccharide, the orange peel pectin and the auxiliary materials, then sterilizing, adding the animal bifidobacterium and the lactobacillus acidophilus, and uniformly mixing.
The sterilization is preferably microwave sterilization, the sterilization temperature is preferably 75-80 ℃, and the sterilization time is preferably 2-3 min.
wherein, the stirring and mixing time is preferably 3-5 min.
The invention separates dietary fiber needed to be supplemented according to different requirements of human bodies day and night and combines the dietary fiber with the dietary fiber. The daily dietary fiber solid beverage mainly contains insoluble dietary fibers which do not participate in the circulation of body fluid and blood of a human body, has good water-absorbing and swelling effects, can reduce the retention time of excrement in intestinal tracts, increases the volume of excrement, and plays roles in regulating intestinal flora and preventing colon cancer; the night dietary fiber solid beverage mainly comprises soluble dietary fibers and probiotics, the soluble dietary fibers are higher in water-soluble viscosity after being dissolved in water, the function in preventing and treating chronic diseases is higher, the soluble dietary fibers can participate in blood and body fluid circulation of a human body, the blood and various organs of the human body can be adsorbed and purified in the circulation process, the health of digestive intestinal tracts is improved while cholesterol in the blood is not increased, and the added probiotics can further regulate intestinal flora to balance the healthy intestinal tract function.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The positive progress effects of the invention are as follows: according to different requirements of a human body in day and night, dietary fibers to be supplemented are separated, the daily dietary fiber solid beverage has the beneficial effects of promoting intestinal tract motility, facilitating discharge of harmful substances in the digestive tract and preventing constipation and colon cancer, and the soluble dietary fibers in the night dietary fiber solid beverage are matched with probiotics and orange peel pectin, so that the effects of clearing intestines, relaxing bowels, controlling blood sugar and cholesterol levels, regulating intestinal flora, promoting intestinal tract motility, losing weight, beautifying and preventing colon cancer are achieved after the night dietary fiber solid beverage is eaten, and the daily dietary fiber solid beverage is an ideal human dietary fiber supplement. The preparation process has simple route and convenient operation.
Drawings
FIG. 1 is a graph showing the effect of different pectin concentrations of orange peel on proliferation of colon cancer cells HT 29.
FIG. 2 is a graph showing the effect of different pectin concentrations of orange peel on proliferation of colon cancer cells Caco 2.
FIG. 3 is a graph plotting the effect of different orange peel pectin concentrations on the proliferation of human normal colonic epithelial cells NCM 460.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
When the daily dietary fiber solid beverage and the night dietary fiber solid beverage in the following examples are eaten, 8g of the solid beverage is dissolved in 250ml of water, namely, the solid beverage is eaten after the dilution ratio is 31.25 times.
Example 1
Mixing 25Kg of wheat fiber powder, 15Kg of soybean fiber powder, 3.5Kg of sweet potato fiber powder, 2Kg of corn fiber powder, 10Kg of orange peel pectin, 5Kg of konjac flour, 9Kg of microcrystalline cellulose, 0.5Kg of aspartame, 18Kg of resistant dextrin and 12Kg of lemon essence in a mixer according to a formula proportion for 3-5 min, then sterilizing (microwave sterilization at 75 ℃ for 3min) and packaging the finished product to prepare the daily dietary fiber solid beverage.
Example 2
20.75Kg of wheat fiber powder, 5Kg of soybean fiber powder, 5Kg of sweet potato fiber powder, 20Kg of corn fiber powder, 5Kg of orange peel pectin, 10Kg of konjac flour, 5Kg of guar gum, 5Kg of sodium carboxymethylcellulose, 0.25Kg of sucralose, 10Kg of resistant dextrin and 10Kg of apple essence are put into a stirrer according to the formula proportion for mixing for 3-5 min, and then are sterilized (microwave sterilization, sterilization temperature of 77 ℃ and sterilization time of 3min) and packaged into finished products to prepare the daily dietary fiber solid beverage.
Example 3
30Kg of wheat fiber powder, 20Kg of soybean fiber powder, 10Kg of sweet potato fiber powder, 5Kg of corn fiber powder, 15Kg of orange peel pectin, 5Kg of konjac flour, 1Kg of guar gum, 1Kg of microcrystalline cellulose, 0.25Kg of sucralose, 5Kg of resistant dextrin and 7.75Kg of apple essence are put into a mixer according to the formula proportion for mixing for 3-5 min, and then are sterilized (microwave sterilization, sterilization temperature of 80 ℃ and sterilization time of 2min) and packaged into finished products to prepare the daily dietary fiber solid beverage.
Example 4
20Kg of resistant dextrin, 20Kg of L-arabinose, 30Kg of xylo-oligosaccharide, 10Kg of isomaltooligosaccharide, 10Kg of orange peel pectin, 0.5Kg of sucralose and 6.5Kg of microcrystalline cellulose are put into a stirrer according to the formula proportion for mixing for 3-5 min, and then the sterilization is carried out (microwave sterilization, the sterilization temperature is 76 ℃, and the sterilization time is 3 min). And finally adding 1.5Kg of animal bifidobacterium and 1.5Kg of lactobacillus acidophilus powder into a stirrer for uniformly mixing, and packaging a finished product to prepare the night dietary fiber solid beverage.
Example 5
50Kg of resistant dextrin, 10Kg of L-arabinose, 15Kg of xylo-oligosaccharide, 5Kg of isomaltooligosaccharide, 5Kg of orange peel pectin, 10Kg of sodium carboxymethylcellulose and 1Kg of apple essence are put into a stirrer according to the formula proportion for mixing for 3-5 min, and then are sterilized (microwave sterilization, the sterilization temperature is 79 ℃, and the sterilization time is 2 min). And finally adding 1Kg of animal bifidobacterium and 4Kg of lactobacillus acidophilus powder into a stirrer to be uniformly mixed, and packaging the finished product to prepare the night dietary fiber solid beverage.
Example 6
70Kg of resistant dextrin, 0.5Kg of L-arabinose, 0.5Kg of xylo-oligosaccharide, 2.5Kg of isomaltooligosaccharide, 5Kg of orange peel pectin, 10Kg of guar gum, 5Kg of microcrystalline cellulose and 0.5Kg of sucralose are put into a stirrer according to the formula proportion for mixing for 3-5 min, and then the sterilization is carried out (microwave sterilization, the sterilization temperature is 76 ℃, and the sterilization time is 3 min). And finally adding 5Kg of animal bifidobacterium and 1Kg of lactobacillus acidophilus powder into a stirrer to be uniformly mixed, and packaging the finished product to prepare the night dietary fiber solid beverage.
Effect example 1
The influence of orange peel pectin on the proliferation of colon cancer cells HT29, Caco2 and human normal colon epithelial cells NCM460 is detected by MTT experiment.
The experimental method comprises the following steps: HT29 colon cancer cells (ATCC company) were cultured in a cell culture incubator at 37 ℃ under 5% CO2 using McCoy's 5A medium (containing 10% fetal bovine serum); caco2 colon cancer cells (ATCC company) were cultured in a cell culture chamber at 37 ℃ under 5% CO2 using DMEM high-sugar medium (containing 10% fetal bovine serum); human normal colon epithelial cells NCM460(Incell corporation) used M3: 10TM medium (containing 10% fetal bovine serum) was cultured in a cell culture incubator at 37 ℃ under 5% CO 2. When the confluence reached 95%, cells were digested with 0.25% trypsin and plated at 5000 cells/well in 96-well cell culture plates in 100. mu.l per well. After the cells adhered overnight, 0, 50, 100, 200, 500, 1000. mu.g/ml of orange peel pectin was added, and the culture was continued for 72 hours. Mu.l of MTT solution 5mg/ml was added to each well. After further culturing for 4 hours, the culture was terminated and the culture medium in the wells was aspirated. Mu.l of dimethyl sulfoxide was added to each well, the mixture was shaken on a shaker at a low speed for 10min to dissolve the crystals sufficiently, OD 490 was measured in each well using an enzyme linked immunosorbent assay (Novostar, BMG labtech Co.), and the cell growth inhibition rate was calculated by recording the results.
The results are shown in fig. 1, 2 and 3, and fig. 1 shows that the orange peel pectin has a relatively obvious effect of inhibiting the proliferation of colon cancer cells HT29, particularly at a concentration of 200 mug/ml. FIG. 2 shows that orange peel pectin also has a relatively obvious effect of inhibiting the proliferation of colon cancer cells Caco2, and particularly has a relatively obvious effect of inhibiting the proliferation of the colon cancer cells at a concentration of more than 200 mu g/ml. However, in human normal colonic epithelial cells NCM460, FIG. 3 shows that orange peel pectin at different doses had no significant effect on its proliferation. The results show that the orange peel pectin has a good targeting anti-tumor effect on colon cancer cells and basically has no influence on normal colon epithelial cells of human.
The daily dietary fiber solid beverage and the night dietary fiber solid beverage both contain orange peel pectin, so the daily dietary fiber solid beverage and the night dietary fiber solid beverage have good targeting anti-tumor effects on colon cancer cells.
Effect example 2
Regulating effect of daily dietary fiber solid beverage on normal mouse intestinal flora
The experimental method comprises the following steps: BALB/c male mice were collected in 48 mice, weighing 18-22g, and randomly divided into 4 groups of 12 mice each. The four groups are respectively blank control group, daily dietary fiber solid beverage low, medium and high dosage groups, and are administrated by intragastric administration for 14 days at 8 am. The control group was given distilled water, and the daily dietary fiber solid beverage was administered at 1.33g/kg BW/day, 2.66g/kg BW/day and 5.32g/kg BW/day (10, 20 and 40 times the recommended dose for human body), respectively, in the low, medium and high dose groups. The dietary fiber solid beverage formulation for daily use was as described in example 1. Prior to administration of the samples, 0.1g of mouse feces was aseptically collected and serially diluted 10-fold and inoculated on each medium. After culturing, colonies are identified and counted by colony morphology, gram stain, biochemical reaction, and the like. After 24 hours from the last sample application, 0.1g of feces was diluted and cultured to count colonies. The identified species include bifidobacteria, lactobacilli, enterobacteria and enterococci. The bifidobacterium is cultured in a bifidobacterium selective medium at 37 ℃ for 48 hours in an anaerobic way; the lactobacillus is cultured in lactobacillus selective medium at 37 deg.c for 48 hr in facultative anaerobic mode; the enterobacteria are cultured for 24 hours at 37 ℃ by using an enterobacteria selective medium; enterococcus was cultured in sodium azide-crystal violet-esculin medium at 37 ℃ for 24 hours.
As shown in the following Table 1, the low, medium and high dose groups of the dietary fiber solid beverage for daily use were able to significantly increase the amount of bifidobacteria and lactobacilli in the intestinal tract, without significant changes in the amount of enterobacteria and enterococci, compared to the control group and the results before the administration. The daily dietary fiber solid beverage has the effects of promoting the proliferation of beneficial flora in intestinal tracts of mice and limiting the proliferation of harmful flora, thereby having the function of regulating the intestinal flora. Similarly, the products of examples 2 and 3 also have a modulating effect on the intestinal flora.
Table 1 results of intestinal flora detection of each group of mice (Mean ± s.d., Log cfu/g wet stool, n ═ 12)
Effect example 3
Regulating effect of night dietary fiber solid beverage on intestinal flora of normal mice
The experimental method comprises the following steps: BALB/c male mice were collected in 48 mice, weighing 18-22g, and randomly divided into 4 groups of 12 mice each. The four groups are respectively blank control group, night dietary fiber solid beverage low, medium and high dose group, and are administrated by gastric gavage for 14 days at 8 nights. The low, medium and high dose groups of the night-use dietary fiber solid beverage were 1.33g/kg BW/day, 2.66g/kg BW/day and 5.32g/kg BW/day, respectively, with distilled water in the control group (10, 20 and 40 times the recommended dose for human body, respectively). The night dietary fiber solid beverage formulation is described in example 4. Prior to administration of the samples, 0.1g of mouse feces was aseptically collected and serially diluted 10-fold and inoculated on each medium. After culturing, colonies are identified and counted by colony morphology, gram stain, biochemical reaction, and the like. After 24 hours from the last sample application, 0.1g of feces was diluted and cultured to count colonies. The identified species include bifidobacteria, lactobacilli, enterobacteria and enterococci. The bifidobacterium is cultured in a bifidobacterium selective medium at 37 ℃ for 48 hours in an anaerobic way; the lactobacillus is cultured in lactobacillus selective medium at 37 deg.c for 48 hr in facultative anaerobic mode; the enterobacteria are cultured for 24 hours at 37 ℃ by using an enterobacteria selective medium; enterococcus was cultured in sodium azide-crystal violet-esculin medium at 37 ℃ for 24 hours.
The results are shown in the following table 2, and compared with the control group and the results before the administration, the low, medium and high dose groups of the night dietary fiber solid beverage can obviously increase the number of bifidobacteria and lactobacilli in intestinal tracts, and the number of enterobacteria and enterococci is not obviously changed. The night dietary fiber solid beverage has the effects of promoting the proliferation of beneficial flora in intestinal tracts of mice and limiting the proliferation of harmful flora, thereby having the function of regulating the intestinal flora. Similarly, the products of examples 5 and 6 also had a modulating effect on the intestinal flora.
Table 2 results of intestinal flora detection of each group of mice (Mean ± s.d., Log cfu/g wet stool, n ═ 12)
Effect example 4
Influence of daily dietary fiber solid beverage on normal mouse small intestine peristalsis function
The experimental method comprises the following steps: taking 72 adult BALB/c male mice, weighing 18-22g, randomly dividing into 6 groups, each group containing 12 mice, and respectively making into negative control group, constipation model group, positive control group, and daily dietary fiber solid beverage low, medium and high dosage groups. Distilled water is given to a control group and a constipation model group, 3.33ml/kg BW ageratum vital qi oral liquid is given to a positive control group, 1.33g/kg BW/day, 2.66g/kg BW/day and 5.32g/kg BW/day (10, 20 and 40 times of the recommended dose of a human body) are respectively given to a low dose group, a medium dose group and a high dose group of a daily dietary fiber solid beverage, and the stomach irrigation is adopted for 15 days at 8 am. The dietary fiber solid beverage formulation for daily use was as described in example 1. After 15 days of continuous administration, mice were fasted for 16 hours without water deprivation, and except for the negative control group, which was administered with the same volume of distilled water by gavage, the mice in each group were administered with 5mg/kg BW of compound diphenoxylate by gavage. After 30min, ink (containing 5% of activated carbon powder and 10% of gum arabic) containing the corresponding sample was administered to the positive control group and 3 administration groups, and ink was administered to the negative control group and the constipation model group. Immediately cutting off the neck after 25 minutes to kill the mouse, dissecting and separating mesentery, cutting out the pylorus to the ileocecal small intestine, placing the pylorus on a tray, slightly pulling the small intestine to be in a straight line, measuring the total length of the small intestine and the advancing length of the ink (pylorus to the front edge of the ink), and calculating the advancing rate of the ink according to the following formula: the ink propulsion rate (%) — the ink propulsion length (cm)/total small intestine length (cm) × 100%. The experimental result is shown in table 3, which indicates that the daily dietary fiber solid beverage can promote the movement of the small intestine of the mouse in a concentration gradient-dependent manner under three dosage concentrations. Similarly, the products of examples 2 and 3 also promote the motility of the small intestine of mice.
Table 3 groups of mice ink propulsion results (Mean ± s.d., n ═ 12)
Note: p <0.05, p <0.01, compared to model group
Effect example 5
Influence of night dietary fiber solid beverage on intestinal peristalsis function of normal mice
The experimental method comprises the following steps: taking 72 adult BALB/c male mice, weighing 18-22g, randomly dividing into 6 groups, each group containing 12 mice, and respectively making into negative control group, constipation model group, positive control group, and night dietary fiber solid beverage low, medium and high dose groups. Distilled water is given to a control group and a constipation model group, 3.33ml/kg BW ageratum zhengqi oral liquid is given to a positive control group, 1.33g/kg BW/day, 2.66g/kg BW/day and 5.32g/kg BW/day (10 times, 20 times and 40 times of the recommended dose of a human body) are respectively given to a low dose group, a middle dose group and a high dose group of the night dietary fiber solid beverage, and the gavage administration is carried out for 15 days at 8 nights. The night time dietary fiber solid beverage component formulation is described in example 4. After 15 days of continuous administration, mice were fasted for 16 hours without water deprivation, and except for the negative control group, which was administered with the same volume of distilled water by gavage, the mice in each group were administered with 5mg/kg BW of compound diphenoxylate by gavage. After 30min, ink (containing 5% of activated carbon powder and 10% of gum arabic) containing the corresponding sample was administered to the positive control group and 3 administration groups, and ink was administered to the negative control group and the constipation model group. Immediately cutting off the neck after 25 minutes to kill the mouse, dissecting and separating mesentery, cutting out the pylorus to the ileocecal small intestine, placing the pylorus on a tray, slightly pulling the small intestine to be in a straight line, measuring the total length of the small intestine and the advancing length of the ink (pylorus to the front edge of the ink), and calculating the advancing rate of the ink according to the following formula: the ink propulsion rate (%) — the ink propulsion length (cm)/total small intestine length (cm) × 100%. Experimental results As shown in Table 4, the night type dietary fiber solid beverage can promote the movement of the small intestine of the mouse in a concentration gradient-dependent manner at three dosage concentrations. Similarly, the products of examples 5 and 6 can also promote the motility of the small intestine of mice.
Table 4 groups of mice ink propulsion results (Mean ± s.d., n ═ 12)
Note: p <0.05, p <0.01, compared to model group
Various modifications and improvements of the technical solutions of the present invention may be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention should fall within the scope of the protection defined by the present invention.
Claims (8)
1. The daily dietary fiber solid beverage is characterized in that raw materials of the daily dietary fiber solid beverage comprise, by mass, 20% ~ 70% of insoluble fiber powder, 0.1% ~ 20% of orange peel pectin, 1% ~ 30% of konjac flour, 1% ~ 30% of resistant dextrin and 1% ~ 30% of auxiliary materials, wherein the percentages are mass percentages relative to the total mass of the raw materials;
The insoluble fiber powder comprises, by mass, ~ 80% of wheat fiber powder, ~ 40% of soybean fiber powder, 1% of ~ 20% of sweet potato fiber powder and 1% of ~ 20% of corn fiber powder.
2. The daily dietary fiber solid beverage as claimed in claim 1, wherein the raw materials of the daily dietary fiber solid beverage comprise 20% ~ 70% of insoluble fiber powder, 0.1% ~ 20% of orange peel pectin, 1% ~ 30% of konjac flour, 1% ~ 30% of resistant dextrin and 1% ~ 30% of auxiliary materials, wherein the percentages are mass percentages relative to the total mass of the raw materials.
3. The daily dietary fiber solid beverage as claimed in claim 1, wherein the auxiliary materials are one or more of edible essence, sour agent and thickener.
4. The daily dietary fiber solid beverage according to claim 3, wherein the flavoring essence is one or more of apple essence, lemon essence and orange essence; the thickening agent is one or more of guar gum, pectin, microcrystalline cellulose, sodium alginate and sodium carboxymethyl cellulose; the sour agent is one or more of citric acid, malic acid and lactic acid.
5. the daily dietary fiber solid beverage according to claim 1, wherein the auxiliary material further comprises a sweetener.
6. The daily dietary fiber solid beverage according to claim 5, wherein the sweetener is one or more of sucralose, aspartame, sodium cyclamate and neotame.
7. A method for preparing a dietary fiber solid beverage for daily use according to any one of claims 1 ~ 6, which comprises mixing insoluble fiber powder, orange peel pectin, konjac flour, resistant dextrin and adjuvants under stirring, and sterilizing.
8. The method of claim 7, wherein the sterilization is microwave sterilization, the sterilization temperature is 75 ~ 80 ℃, and the sterilization time is 2 ~ 3 min;
And/or the stirring and mixing time is 3-5 min.
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