CN106460051A - 集成的单细胞测序 - Google Patents

集成的单细胞测序 Download PDF

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Publication number
CN106460051A
CN106460051A CN201580024133.5A CN201580024133A CN106460051A CN 106460051 A CN106460051 A CN 106460051A CN 201580024133 A CN201580024133 A CN 201580024133A CN 106460051 A CN106460051 A CN 106460051A
Authority
CN
China
Prior art keywords
cdna
sequence
rna
nucleic acid
forming
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580024133.5A
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English (en)
Chinese (zh)
Inventor
詹森·A·A·韦斯特
布赖恩·福勒
曾子豪
克里斯蒂安·F·约翰逊
马克·A·恩格
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Standard Biotools Inc
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Publication of CN106460051A publication Critical patent/CN106460051A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580024133.5A 2014-05-08 2015-05-08 集成的单细胞测序 Pending CN106460051A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201461990598P 2014-05-08 2014-05-08
US61/990,598 2014-05-08
US201462079495P 2014-11-13 2014-11-13
US62/079,495 2014-11-13
PCT/US2015/029986 WO2015172080A1 (en) 2014-05-08 2015-05-08 Integrated single cell sequencing

Publications (1)

Publication Number Publication Date
CN106460051A true CN106460051A (zh) 2017-02-22

Family

ID=54393068

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580024133.5A Pending CN106460051A (zh) 2014-05-08 2015-05-08 集成的单细胞测序

Country Status (6)

Country Link
US (2) US20150337295A1 (https=)
EP (1) EP3140428B1 (https=)
JP (1) JP6716465B2 (https=)
CN (1) CN106460051A (https=)
SG (2) SG10202004286UA (https=)
WO (1) WO2015172080A1 (https=)

Cited By (1)

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CN112840023A (zh) * 2018-10-25 2021-05-25 Illumina公司 使用索引和条形码识别阵列上的配体的方法和组合物

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CN106811460B (zh) * 2015-11-30 2020-11-27 浙江安诺优达生物科技有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
EP3467121A4 (en) * 2016-05-25 2020-02-12 Nikon Corporation METHOD FOR IDENTIFYING TARGET BIOLOGICAL, BALLS FOR USE IN IDENTIFYING TARGET BIOLOGICAL, BALL SET, AND DEVICE FOR IDENTIFYING TARGET BIOLOGICAL
EP3775196A4 (en) * 2018-04-04 2021-12-22 Nautilus Biotechnology, Inc. METHOD FOR GENERATING NANOARRAYS AND MICROARRAYS
US10881692B2 (en) 2018-04-05 2021-01-05 Medeor Therapeutics, Inc. Compositions for establishing mixed chimerism and methods of manufacture thereof
US10842821B2 (en) 2018-04-05 2020-11-24 Medeor Therapeutics, Inc. Cellular compositions derived from prior organ donors and methods of manufacture and use thereof
EP3917660A4 (en) * 2019-01-29 2022-11-23 Molecular Assemblies, Inc. REUSABLE INITIATORS FOR NUCLEIC ACID SYNTHESIS
WO2023102141A2 (en) * 2021-12-02 2023-06-08 Bio-Rad Laboratories, Inc. Use of dna:rna duplex fragmentation

Citations (1)

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US20110059556A1 (en) * 2009-09-04 2011-03-10 The Research Foundation Of State University Of New York Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices

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JP2002503954A (ja) * 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
GB9714716D0 (en) * 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
CA2798625C (en) * 2000-04-17 2017-01-24 Dyax Corp. Novel methods of constructing libraries of genetic packages that collectively display the members of a diverse family of peptides, polypeptides or proteins
AUPQ974900A0 (en) * 2000-08-29 2000-09-21 Macquarie Research Limited Degenerate oligonucleotide gene-shuffling
US20040209263A1 (en) * 2000-12-07 2004-10-21 Clawson Gary A. Selection of catalytic nucleic acids targeted to infectious agents
JP2004097158A (ja) * 2002-09-12 2004-04-02 Kureha Chem Ind Co Ltd 発現遺伝子同定用ｃＤＮＡタグの作成方法、及び該ｃＤＮＡタグを用いる遺伝子発現解析方法
CA2648149A1 (en) * 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
WO2009124255A2 (en) 2008-04-04 2009-10-08 Helicos Biosciences Corporation Methods for transcript analysis
US20100035249A1 (en) * 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
CN104350374B (zh) 2012-02-29 2018-04-27 富鲁达公司 用于使用微流体的多个单细胞捕获和处理的方法、系统和设备
NO2694769T3 (https=) 2012-03-06 2018-03-03
CN105164246B (zh) 2013-03-15 2018-09-25 富鲁达公司 用于分析定义的多细胞组合的方法和设备

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DYNAL BIOTECH: "gene expression in focus", 《DOC88.COM/P-9731851787168.HTML》 *
GEORGE GEORGIOU等: "The promise and challenge of high-throughput sequencing of the antibody repertoire", 《NATURE BIOTECHNOLOGY》 *
SONIA SCHOONBROODT等: "Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library", 《NUCLEIC ACIDS RESEARCH》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112840023A (zh) * 2018-10-25 2021-05-25 Illumina公司 使用索引和条形码识别阵列上的配体的方法和组合物
CN112840023B (zh) * 2018-10-25 2024-03-01 Illumina公司 使用索引和条形码识别阵列上的配体的方法和组合物

Also Published As

Publication number Publication date
EP3140428A4 (en) 2018-02-28
JP6716465B2 (ja) 2020-07-01
JP2017514478A (ja) 2017-06-08
EP3140428A1 (en) 2017-03-15
EP3140428B1 (en) 2021-07-28
US20150337295A1 (en) 2015-11-26
SG10202004286UA (en) 2020-06-29
WO2015172080A1 (en) 2015-11-12
SG11201609055RA (en) 2016-11-29
US20190249238A1 (en) 2019-08-15

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