CN106442915A - Test method for oxygen consumption of fish - Google Patents
Test method for oxygen consumption of fish Download PDFInfo
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- CN106442915A CN106442915A CN201610961745.3A CN201610961745A CN106442915A CN 106442915 A CN106442915 A CN 106442915A CN 201610961745 A CN201610961745 A CN 201610961745A CN 106442915 A CN106442915 A CN 106442915A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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Abstract
The invention relates to a test method for oxygen consumption of fish. The method comprises the following steps: 1) culturing fish in a controlled airtight experiment cabin, and removing azolla and vegetables stored in the controlled airtight experiment cabin in advance out of the controlled airtight experiment cabin; 2) turning off an artificial light source in the controlled airtight experiment cabin, and starting an online monitoring system device; 3) keeping the experiment for 7 days, and daily measuring the O2 and CO2 concentration in atmosphere for one time; and 4) respectively drawing the data of the daily measured O2 and CO2 concentration into a table, and performing linear analysis. The test method is simple and convenient and can be used for conveniently monitoring the influence of the fish culture on the atmospheric environment in the controlled airtight experiment cabin.
Description
Technical field
The present invention relates to a kind of fish oxygen consumption method of testing.
Background technology
Controlled ecological life support system（Abbreviation CELSS）, it is that the mankind carry out following long-time, remote, many occupant's load
The AS that people's solar-system operation can be trusted.And people will appear from low O in closed environment2Concentration and high CO2Concentration environment, fish
Whether class cultivation also has low O2Concentration and high CO2The phenomenon of concentration environment.For this reason, it may be necessary to a kind of fish oxygen consumption method of testing.
Content of the invention
It is an object of the invention to provide a kind of fish oxygen consumption method of testing, the method for testing simplicity, are conveniently used for monitoring fish
Class cultivates the impact to atmospheric environment in controlled closed cabin.
Technical program of the present invention lies in：A kind of fish oxygen consumption method of testing, comprises the following steps：
1）Cultivate fish in the controlled closed Laboratory Module, and the red duckweed that was originally placed in controlled closed Laboratory Module and vegetable remove
In controlled closed Laboratory Module；
2）The artificial light source that closes in controlled closed Laboratory Module, and start on-line monitoring system device；
3）Therapy lasted 7 days, determines 1 cabin atmosphere O daily2And CO2Concentration；
4）By the daily O for determining2And CO2Concentration data are depicted as table respectively, and carry out linear analysiss.
Further, the controlled closed Laboratory Module includes the nacelle being made up of front, rear, left and right side plate and ceiling, described
The each side plate of nacelle is assembled by the splicing of two-sided membrane color steel respectively, and each side plate lower end of nacelle is equipped with and ground respectively
The groove aluminum being connected, the ceiling is spliced by the stupefied plate of color steel tile, is located on the downside of ceiling and is provided with furred ceiling in the nacelle, institute
Furred ceiling is stated by the splicing assembling of two-sided membrane color steel.
Further, the connecting end surface of the adjacent biside plate of the nacelle is in 45 ° respectively and cooperatively forms 90 ° of angles, and phase
In the gap of the counterface of adjacent biside plate, live pointing has foaming thermal-insulating, riveting at the external corners that adjacent biside plate is formed
Aluminum corner brace is connected to, at the inside corner that adjacent biside plate is formed, arc is snapped with, the aluminum corner brace and arc are with biside plate respectively
End count sealing jointing.
Further, the top of the left plate and right plate is respectively arranged with and is matched therewith to form grafting space
Two aluminum corner braces, the outer end of second swash plate is embedded in grafting space, is filled with foaming thermal-insulating in the grafting space, and described the
Gap filling between two aluminum corner braces and the upper side of the second swash plate has a fluid sealant, the downside of second swash plate and left plate or
The corner that the medial surface of right plate is formed has buckled the second arc.
Further, be filled with fluid sealant between the groove aluminum and the bottom of each side plate, described two second swash plates interior
Side upper surface connects through the third angle aluminium phase, is filled with foaming thermal-insulating in the gap between two second swash plates.
Further, the top of the forward and backward side plate be respectively arranged be secured to connection and for the second swash plate before
The second embedded groove aluminum of side or rear side end, the upside gap filling that the second groove aluminum is matched with front side board or back side panel has
Fluid sealant, second swash plate underside corner to be formed that matches with front side board or back side panel is provided with the 3rd arc.
Further, the fluid sealant is butyl sealant, and the foaming thermal-insulating is foamed glue.
Further, the on-line monitoring system device includes to circulate air pump, and the inlet end of the circulation air pump is through first
Ball valve is connected with the first pipeline for passing controlled closed test, circulate air pump the second ball valve of outlet side with penetrate controlled
The second pipeline in closed Laboratory Module is connected, and the output port of second pipeline is arranged at installed in controlled closed Laboratory Module
Above interior culturing pool, in the culturing pool, water circulating pump is provided with, the water circulating pump is through the 3rd pipeline and the 3rd ball valve phase
Connection, the 3rd ball valve is connected with the second ball valve through the 4th ball valve, is also set up between the 3rd ball valve and the 4th ball valve
Have the first interface being connected with the sampler of water-quality determination instrument, be additionally provided between the circulation air pump and the second ball valve with
The second interface that the sampler of atmospheric analysis instrument is connected.
Further, the 5th ball valve being provided with the first interface, is provided with the 6th ball valve in the second interface.
Further, the output port of second pipeline is arranged at the 140 ~ 160mm of the water surface apart from culturing pool.
Compared with prior art, the present invention has advantages below：The method of testing simplicity, is conveniently used for monitoring Fish and supports
Grow the impact to atmospheric environment in controlled closed cabin, so as to be to the manned confession O in next stage closed cabin2Research provides help.
Meanwhile, controlled closed Laboratory Module belongs to phaeism, is not affected with solar radiation by earth surface round the clock, experimental system and natural ring
Border completely cuts off, the internal closed cycle for realizing empty gas and water, the also all manually light source regulation and control of the illumination needed for the implants of cabin, favorably
In simulation space circadian；Nacelle adopts two-sided membrane color steel material, and the material possesses good heat preservation and insulation, material
Light weight, good stability, indeformable, it is easy to processing and installation；Especially the material is nonhygroscopic, is conducive to the collection of condensed water in cabin
And recycling.
Description of the drawings
Fig. 1 is the structural representation of the controlled closed Laboratory Module of the present invention；
Fig. 2 is the A area enlarged drawing of Fig. 1 of the present invention；
Fig. 3 is the B area enlarged drawing of Fig. 1 of the present invention；
Fig. 4 is the C area enlarged drawing of Fig. 1 of the present invention；
Fig. 5 is the D area enlarged drawing of Fig. 1 of the present invention；
Fig. 6 is the E area enlarged drawing of Fig. 1 of the present invention；
Fig. 7 is the structural representation of the on-line monitoring system device of the present invention；
Fig. 8 is the controlled closed experiment cabin atmosphere O of the present invention2And CO2Concentration mensuration table；
Fig. 9 is the fish culture of the present invention to closed cabin atmosphere O2The impact situation map of concentration change；
Figure 10 is the fish culture of the present invention to Atmospheric CO in closed cabin2The impact situation map of concentration change；
In figure：A- front side board c- left plate d- right plate 1- groove aluminum 2- ceiling 2a- swash plate 2b- swash plate 2c-
Angle 3- furred ceiling 3a- the second swash plate 3b- the second swash plate 3c- angle 4- foaming thermal-insulating 5- aluminum corner brace 6- arc
The 3rd arc of shape plate 7- fluid sealant 8- the second aluminum corner brace 9- the second arc 10- the third angle aluminum 11- the second groove aluminum 12-
The controlled closed test 21- of shape plate 13- ground 20- circulates air pump 22- the first ball valve 23- the first pipeline 24-
The 3rd ball valve 30- of second the 3rd pipeline 29- of ball valve 25- the second pipeline 26- culturing pool 27- water circulating pump 28-
The 6th ball valve of 4th the 5th ball valve 34- of ball valve 31- first interface 32- second interface 33-.
Specific embodiment
It is that the features described above of the present invention and advantage can be become apparent, special embodiment below, and coordinate accompanying drawing, make detailed
Carefully it is described as follows, but the present invention is not limited to this.
Referring to figs. 1 to Figure 10
A kind of fish oxygen consumption method of testing, comprises the following steps：
1）Cultivate fish in the controlled closed Laboratory Module, and the red duckweed that was originally placed in controlled closed Laboratory Module and vegetable remove
In controlled closed Laboratory Module；
2）The artificial light source that closes in controlled closed Laboratory Module, and start on-line monitoring system device；
3）Therapy lasted 7 days, determines 1 cabin atmosphere O daily2And CO2Concentration；
4）By the daily O for determining2And CO2Concentration data are depicted as table respectively, and carry out linear analysiss.
In the present embodiment, the controlled closed Laboratory Module is included by front side board a, back side panel, left plate c, right plate b and top
The nacelle of canopy composition, each side plate of the nacelle is assembled by the two-sided membrane color steel splicing of width S=100mm respectively, and
Each side plate lower end of nacelle is equipped with the groove aluminum 1 being connected with ground 13 respectively, and the ceiling 2 is by two swash plates that angle is obtuse angle
2a, 2b constitute, and the swash plate is spliced by the stupefied plate of color steel tile of width S=50mm, be located on the downside of ceiling and set in the nacelle
Be equipped with furred ceiling 3, the furred ceiling is made up of two second swash plate 3a, 3b that angle is obtuse angle, second swash plate by width S=
The two-sided membrane color steel splicing composition of 150mm, the steel construction frame that the ceiling is made by 50mm × 50mm × 3mm square tubes soldering
Frame is supported；Each side plate is also placed in inside the general frame of nacelle.
In the present embodiment, the angle 2c of the ceiling is 130 °, and the angle 3c of the furred ceiling is 160 °, and furred ceiling has 10 °
Gradient, shows preferable stability；Meanwhile, condensed water will not be concentrated on furred ceiling, it is to avoid continuous damage of the water droplet to cabin implants
Wound.
In the present embodiment, the connecting end surface of the adjacent biside plate of the nacelle in 45 ° and cooperatively forms 90 ° of angles respectively, and
In the gap of the counterface of adjacent biside plate, live pointing has foaming thermal-insulating 4, the external corners that adjacent biside plate is formed
Place's riveting has aluminum corner brace 5, is snapped with arc 6, the aluminum corner brace and arc and both sides at the inside corner that adjacent biside plate is formed
Plate 7 jointing of end count sealing respectively, to ensure sealing effectiveness.
In the present embodiment, the top of the left plate and right plate is respectively arranged with and is matched therewith forming grafting space
Second aluminum corner brace 8, the outer end of second swash plate is embedded in grafting space, is filled with foaming thermal-insulating 4, institute in the grafting space
Stating the gap filling between the second aluminum corner brace and the upper side of the second swash plate has fluid sealant 7, the downside of second swash plate and a left side
The corner that the medial surface of side plate or right plate is formed has buckled the second arc 9.
In the present embodiment, fluid sealant 7 between the groove aluminum and the bottom of each side plate, is filled with, described two second swash plates
Medial extremity upper surface is connected through the third angle aluminum 10 in obtuse angle, is filled with foamed heat insulating material in the gap between two second swash plates
Material 4.
In the present embodiment, the top of the forward and backward side plate is respectively arranged with and is secured to connection and for the second swash plate
The second embedded groove aluminum 11 of front side end or rear side end, the upside gap that the second groove aluminum is matched with front side board or back side panel is filled out
Fluid sealant 7 is filled with, second swash plate underside corner to be formed that matches with front side board or back side panel is provided with the 3rd arc
Plate 12.
In the present embodiment, it is foamed glue that the fluid sealant is butyl sealant, the foaming thermal-insulating.Butyl sealant
It is a kind of one-component neutrality fluid sealant with butyl rubber as substrate, with property such as splendid ageing-resistant, corrosion-resistant, resistances to oxidation
Can, the adaptability for interface deformation is very strong, and especially waterproof, sealing, weather-proof performance of following are projected the most.Therefore, to color steel
Sealing protective effect is played at the positions such as plate seaming, perforate, overlap joint.
In the present embodiment, the on-line monitoring system device includes to circulate air pump 21, the inlet end warp of the circulation air pump
First ball valve 22 is connected with the first pipeline 23 for passing controlled closed test 20, and the air inlet port of the first pipeline is positioned at controlled
The center of closed Laboratory Module implants cultivating chamber.Circulate second ball valve of outlet side 24 of air pump and penetrate controlled closed reality
The second pipeline 25 in hatch checking is connected, and the output port of second pipeline is arranged in controlled closed Laboratory Module
26 top of culturing pool, is provided with water circulating pump 27 in the culturing pool, the water circulating pump is through the 3rd pipeline 28 and the 3rd ball valve
29 are connected, and the 3rd ball valve is connected with the second ball valve 24 through the 4th ball valve 30, the 3rd ball valve and the 4th ball valve it
Between be additionally provided with the first interface 31 being connected with the sampler of water-quality determination instrument, between the circulation air pump and the second ball valve
It is additionally provided with the second interface 32 being connected with the sampler of atmospheric analysis instrument.
In the present embodiment, the 5th ball valve 33 is provided with the first interface, is provided with the 6th ball in the second interface
Valve 36.The output port of second pipeline is arranged at the 140 ~ 160mm of the water surface apart from culturing pool.
Determine O in controlled closed experiment cabin atmosphere2- CO2When concentration and volatile organic content, the first ball is first opened
Valve and the second ball valve, close the 6th ball valve and the 4th ball valve, after air pump work 3min to be recycled, then open the 6th ball valve, close
Second ball valve, you can sample from second interface.May insure that acquirement is arranged on controlled closed Laboratory Module interplantation by the program sampling
The center gaseous sample of thing cultivating chamber.Because circulation air pump has circulated 3min in advance before sampling, whole circulation line fills
Full gas ingredients are consistent with the center gas ingredients of plant culture room.When the 6th ball valve is opened sampling, stand
The gas of cultivating chamber center is obtained, therefore sampling amount is considerably less.
When the water quality of breeding water body in controlled closed Laboratory Module is determined, the 3rd ball valve and the 4th ball valve first being opened, closes the
Five ball valves and the second ball valve, after pump working 3min to be recycled, then open the 6th ball valve, close the 4th ball valve, you can from first
Interface is sampled.May insure to obtain the water sample of fish culture great Chi by the program sampling, because water circulating pump is pre- before sampling
Circulation 3min, the water body composition that whole circulation line is full of is consistent with the water body composition of fish culture great Chi.Work as valve
6th ball valve is opened when sampling, and obtains the water body of fish culture great Chi immediately, therefore sampling amount is considerably less.After sampling terminates, the
First, second, third and the 4th ball valve should be located in off position, to guarantee that in closed cabin, gas is not leaked.
Result of the test shows, with continuing for fish culture time, closed cabin atmosphere O2Lowering of concentration, CO2In concentration
Rise.According to the linear regression analyses of Fig. 9 and Figure 10, O2Concentration is declined with daily 0.168% speed, O after 7 days2Concentration is
19.38%；CO2Concentration is risen with daily 0.153% speed, CO after 7 days2Concentration is 1.115%.After test 7 days, fish culture
In water body, ammonia-nitrogen content is sharply increased, and dissolved oxygen content also drastically declines.
Presently preferred embodiments of the present invention is the foregoing is only, for the ordinary skill in the art, according to this
Bright teaching, designs the fish oxygen consumption method of testing of multi-form and does not need performing creative labour, without departing from the present invention's
In the case of principle and spirit, all impartial change, modification, replacement and modifications that is made according to scope of the present invention patent, should all belong to
The covering scope of the present invention.
Claims (10)
1. a kind of fish oxygen consumption method of testing, it is characterised in that comprise the following steps：
1）Cultivate fish in the controlled closed Laboratory Module, and the red duckweed that was originally placed in controlled closed Laboratory Module and vegetable remove
In controlled closed Laboratory Module；
2）The artificial light source that closes in controlled closed Laboratory Module, and start on-line monitoring system device；
3）Therapy lasted 7 days, determines 1 cabin atmosphere O daily2And CO2Concentration；
4）By the daily O for determining2And CO2Concentration data are depicted as table respectively, and carry out linear analysiss.
2. a kind of fish oxygen consumption method of testing according to claim 1, it is characterised in that the controlled closed Laboratory Module includes
The nacelle being made up of front, rear, left and right side plate and ceiling, each side plate of the nacelle is respectively by the splicing assembling of two-sided membrane color steel
Form, and each side plate lower end of nacelle is equipped with the groove aluminum being connected with ground respectively, the ceiling is by the stupefied plate splicing of color steel tile
Form, be located in the nacelle on the downside of ceiling and furred ceiling is provided with, the furred ceiling is by the splicing assembling of two-sided membrane color steel.
3. a kind of fish oxygen consumption method of testing according to claim 2, it is characterised in that the company of the adjacent biside plate of the nacelle
Contact surface is in 45 ° respectively and cooperatively forms 90 ° of angles, and in the gap of the counterface of adjacent biside plate, live pointing has foaming
Insulation material, riveting at the external corners that adjacent biside plate is formed has aluminum corner brace, snapping at the inside corner that adjacent biside plate is formed
There is arc, the aluminum corner brace and arc and biside plate distinguish end count sealing jointing.
4. a kind of fish oxygen consumption method of testing according to claim 3, it is characterised in that the left plate and right plate upper
Portion is respectively arranged with and is matched therewith the second aluminum corner brace to form grafting space, and the outer end of second swash plate is embedded in grafting space,
Foaming thermal-insulating, the gap filling between second aluminum corner brace and the upper side of the second swash plate is filled with the grafting space
There is fluid sealant, the downside of second swash plate has buckled the second arc with the corner of the medial surface formation of left plate or right plate
Shape plate.
5. a kind of fish oxygen consumption method of testing according to claim 4, it is characterised in that the lower end of the groove aluminum and each side plate
Be filled with fluid sealant between portion, the medial extremity upper surface of described two second swash plates connects through the third angle aluminium phase, two second swash plates it
Between gap in be filled with foaming thermal-insulating.
6. a kind of fish oxygen consumption method of testing according to claim 3,4 or 5, it is characterised in that the forward and backward side plate upper
Portion is respectively arranged with and is secured to connect and the second embedded groove aluminum of the front side end for the second swash plate or rear side end, and described second
The upside gap filling that groove aluminum is matched with front side board or back side panel has fluid sealant, second swash plate and front side board or back side panel
The underside corner to be formed that matches is provided with the 3rd arc.
7. a kind of fish oxygen consumption method of testing according to claim 6, it is characterised in that the fluid sealant is butyl sealing
Glue, the foaming thermal-insulating is foamed glue.
8. a kind of fish oxygen consumption method of testing according to claim 1,2,3,4 or 7, it is characterised in that the on-line monitoring
System and device includes to circulate air pump, the inlet end of the circulation air pump through the first ball valve with pass the first of controlled closed test
Pipeline is connected, and the second ball valve of outlet side for circulating air pump is connected with the second pipeline in controlled closed Laboratory Module is penetrated,
The output port of second pipeline is arranged above the culturing pool in installed in controlled closed Laboratory Module, is set in the culturing pool
Be equipped with water circulating pump, the water circulating pump is connected with the 3rd ball valve through the 3rd pipeline, the 3rd ball valve through the 4th ball valve with
Second ball valve is connected, and is additionally provided with and is connected with the sampler of water-quality determination instrument between the 3rd ball valve and the 4th ball valve
First interface, be additionally provided with for being connected with the sampler of atmospheric analysis instrument between the circulation air pump and the second ball valve
Two interfaces.
9. a kind of fish oxygen consumption method of testing according to claim 8, it is characterised in that be provided with the first interface
Five ball valves, are provided with the 6th ball valve in the second interface.
10. a kind of fish oxygen consumption method of testing according to claim 9, it is characterised in that the outfan of second pipeline
Mouth is arranged at the 140 ~ 160mm of the water surface apart from culturing pool.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610961745.3A CN106442915A (en) | 2016-10-28 | 2016-10-28 | Test method for oxygen consumption of fish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610961745.3A CN106442915A (en) | 2016-10-28 | 2016-10-28 | Test method for oxygen consumption of fish |
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| Publication Number | Publication Date |
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| CN106442915A true CN106442915A (en) | 2017-02-22 |
Family
ID=58180433
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610961745.3A Pending CN106442915A (en) | 2016-10-28 | 2016-10-28 | Test method for oxygen consumption of fish |
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Application publication date: 20170222 |