CN106436473A - Preparation method and application of compound chitosan bacteriostatic food wrapping paper - Google Patents
Preparation method and application of compound chitosan bacteriostatic food wrapping paper Download PDFInfo
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- CN106436473A CN106436473A CN201610829620.5A CN201610829620A CN106436473A CN 106436473 A CN106436473 A CN 106436473A CN 201610829620 A CN201610829620 A CN 201610829620A CN 106436473 A CN106436473 A CN 106436473A
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H27/00—Special paper not otherwise provided for, e.g. made by multi-step processes
- D21H27/10—Packing paper
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H19/00—Coated paper; Coating material
- D21H19/10—Coatings without pigments
- D21H19/14—Coatings without pigments applied in a form other than the aqueous solution defined in group D21H19/12
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
- D21H21/14—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties characterised by function or properties in or on the paper
- D21H21/36—Biocidal agents, e.g. fungicidal, bactericidal, insecticidal agents
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- Pest Control & Pesticides (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a preparation method of a compound chitosan bacteriostatic food wrapping paper and application of the compound chitosan bacteriostatic food wrapping paper as a wrapping film in pastry foods and preserved fruit products. By virtue of the preparation method, a mixed solution is prepared from chitosans with different bacteriostatic effects and a deacetylation degree of 95%, chitosan with a molecular weight of 100000 and chitosan hydrochloride, an emulsifying agent Twain and a bacteriostatic agent nisin are added into the mixed solution, and kraft paper is coated with the mixed solution to form a chitosan film with relatively high tensile failure stress and breaking elongation and relatively good bacteriostatic effect. The chitosan film can be used as a wrapping film for wrapping the pastry foods and the preserved fruit products, and the foods can meet state-specified guarantee period requirements without being added with a preservative. The chitosan film is low in production cost, can meet the edible standard, does not affect the quality of the foods at all and is wide in application range.
Description
Technical field
The present invention relates to a kind of chitosan film product；The system of the antibacterial food wrapper of particularly a kind of recombination chitosan
Preparation Method and application.
Background technology
Shitosan is the product of de-acetyl chitin, can prevent and treat the hardening of tremulous pulse medicated porridge, treatment in field of medicaments shitosan
Hyperlipemia, blood coagulation and anticoagulation aspect play the role of very big.And shitosan can be developed to appetrol, it can also simultaneously
As medicament dressing.In field of food, shitosan is good thickening agent, stabilizer, emulsifying agent and preservative.Shitosan itself
The polycation characteristic being had, good filming performance make it the good material preparing food micro capsule.
Shitosan has good inhibition to antibacterial, funguses etc., can make wound dressing materials, hemostatic material with
And operation suture thread etc..Shitosan is easier film forming, and the better mechanical property of chitosan film, and resistance to elevated temperatures is preferable,
Can be made into the film of various uses.
Income with our people improves constantly, and can the requirement to food for the people be no longer Well fed,well bred., it is required that
The green eaten, health.Existing packaging material for food mostly is plastic, and in degradable, safety aspect has a lot of work to want
Do.It is known that the packaging waste producing because of excessive packaging in recent years creates very big burden to environment, therefore can drop
Solution and edible food packaging thin film are increasingly subject to the attention of food worker and the favor of consumer.And shitosan itself
Advantage makes it the raw material of good edible film.
Nisin is the bacteriocin being synthesized by Lactococcus lactis subsp.lactis, and its structure is polypeptide, nisin
Rhzomorph has good fungistatic effect to most of gram positive bacterias, is not very bright to gram negative bacteria and fungi restraining effect
Aobvious.Existing chemical preservative, such as sorbic acid, benzoic acid, nitrous acid all have certain impact to flavour of food products, and overusing can be right
Human body produces harm.Nisin is natural harmless biological preservative, can be by the protease hydrolysiss in human body.Lactic acid chain
Coccus element is added in milk product and can extend its shelf-life after milk product sterilization, reduces loss.Add few in acid canned food
Amount nisin, can reduce sterilization temperature, and can improve the taste of vegetable product.Nisin is also extensively applied
In bakery product, beverage, as preservative in the food such as instant food, its effect is all than more significant.
The biocidal property that shitosan has imparts the excellent antibacterial property of shitosan edible film, can suppress packaging food
The growth of microorganism in product, its characteristic insoluble in hot water and cold water enables it to pack various solid foods.Shitosan is used
Both reduced packaging material for food to the pollution caused by environment in packaging for foodstuff, and so that shrimp and crab shells is reasonably used again,
It is the scheme of a doulbe-sides' victory.Therefore, the preparation method of the new antibacterial food wrapper of research and development has very high industrial application value.
Content of the invention
The technical problem to be solved is for the deficiencies in the prior art, provide a kind of new, tension failure should
Power and elongation at break are higher, fungistatic effect is relatively good, the preparation side of the antibacterial food wrapper of the recombination chitosan of low cost
Method.
Another technical problem to be solved by this invention there is provided the antibacterial food of recombination chitosan that preceding method is obtained
The application of product wrapping paper.
The technical problem to be solved is to be realized by following technical scheme.The present invention is a kind of compound
The preparation method of the antibacterial food wrapper of shitosan, is characterized in, comprises the steps：
(1) shitosan of deacetylation 95% is dissolved in the acetic acid solution of 1% volumetric concentration, is heated to 50 DEG C of stirrings
To uniformly not having granule to obtain solution A, it is configured to the shitosan acetic acid solution of mass fraction 4～6%；
(2) shitosan of molecular weight 100,000 is placed in the acetic acid solution of 1% volumetric concentration, is heated to 50 DEG C and stirs to equal
Even solution B, is configured to mass fraction 0.9～6.3% shitosan acetic acid solution；
(3) chitosan hydrochlorate is placed in the acetic acid solution of 1% volumetric concentration, stirs to dissolving to obtain solution C, prepare dense
Degree 0.9～6.3% shitosan acetic acid solution；
(4) ratio of solution A, solution B and solution C in mass ratio 1: 1: 1 is mixed, add tween and streptococcus acidi lactici
Element, makes tween mass concentration in mixed liquor be 1.0～1.8%, the mass concentration of nisin is：0.02～0.1%, will
Mixed liquor stirs, and only puts 12h degassing, mixed liquor is spread evenly across on kraft paper, using multiple film, first few rear many,
The mode of repeated drying, until mixed liquor is painted with completely, dries 2.5h film forming at 55 DEG C.
Preparation method of the present invention, its further preferred technical scheme or technical characteristic are：
1st, the mass fraction making solution A in mixed liquor is preferably 2%, and the mass fraction of solution B is preferably 0.6%, solution C
Mass fraction be preferably 1.4%.
2nd, the number of times of film at least 5 times, using often painting first drying once by the way of.
3rd, in the mixed solution described in step (4), the mass concentration of tween is preferably 1.6%.
4th, in the mixed solution described in step (4), the mass concentration of nisin is preferably 0.06%.
5th, the film amount described in step (4) is：The kraft paper of every 10cm × 10cm applies 5～7mL mixed liquor, optimum
Elect painting 6mL mixed liquor on the kraft paper of every 10cm × 10cm as.
The antibacterial food wrapper of recombination chitosan that method of the present invention is obtained can be used for moon cake or Apples
The packaging of dried meat.
The following is correlational study experiment and its result that inventor is done.
First, in the present invention, the bacteriostatic activity of Chitosan-phospholipid complex and optimum proportioning determine
1st, the antibacterial result of chitosan hydrochlorate
Chitosan hydrochlorate is as shown in table 1 to the histamine result of five kinds of experimental bacteria, chitosan hydrochlorate as can be seen from Table 1
All there is inhibition to 5 kinds of experimental bacteria, and the inhibition of antibacterial is better than with the inhibition of funguses, with the increase of concentration
It also strengthens to the inhibition of experimental bacteria, and it is best to the colibacillary inhibition of gram negative bacteria.
The histamine result to microorganism for table 1 chitosan hydrochlorate
Note：The a diameter of 6mm of filter paper that bacteriostatic experiment adopts
2nd, the antibacterial result of molecular weight 100,000 shitosan
Molecular weight 100,000 shitosan fungistatic effect is as shown in table 2, its inhibition to bacillus subtilis as can be seen from Table 2
Relatively good, and be better than chitosan hydrochlorate, with concentration increase it the fungistatic effect of 5 kinds of bacterium is all gradually strengthened.
The histamine result to microorganism for table 2 molecular weight 100,000 shitosan
3rd, the antibacterial result of molecular weight 50,000 shitosan
Molecular weight 50,000 shitosan fungistatic effect is as shown in table 3, its inhibition to three kinds of antibacterials as can be seen from Table 3
Slightly poorer than the shitosan of molecular weight 100,000, suitable with the shitosan of molecular weight 100,000 to the inhibition of funguses.
The histamine result to microorganism for table 3 molecular weight 50,000 shitosan
4th, the antibacterial result of deacetylation 90% shitosan
Table 4 is the inhibition to 5 kinds of bacterium for deacetylation 90% shitosan.It is equal to five kinds of bacterium as can be seen from Table 4
There is inhibition, the fungistatic effect of several bacterium is more or less the same it is worth mentioning at this point that it is better than to the inhibition of funguses yeast
Chitosan hydrochlorate and molecular weight 100,000 shitosan.
The histamine result to microorganism for table 4 deacetylation 90% shitosan
5th, the antibacterial result of deacetylation 95% shitosan
Table 5 is the inhibition to 5 kinds of bacterium for deacetylation 90% shitosan.It is equal to five kinds of bacterium as can be seen from Table 5
Have inhibition, with concentration increase its antibacterial circle diameter of several bacterium is all gradually increased, but but concentration be higher than 2.5% when suppression
The increase of bacterium loop diameter is inconspicuous, or even declines, and reason is probably that diffusion effect is not good.
The histamine result to microorganism for table 5 deacetylation 95% shitosan
2nd, the selection of composite
Film is carried out to kraft paper, plastic sheeting, it was found that applying shape after chitosan solution drying on a plastic film
The film periphery becoming curls tilting, easily separates with plastic sheeting, so plastic sheeting is not suitable for as the answering of shitosan in this experiment
Condensation material.When chitosan solution is applied on kraft paper, because the space that Corii Bovis seu Bubali paper structure exists can absorb a small amount of chitosan solution,
Well and film combination can form tight structure after the drying, therefore select kraft paper as composite.
3rd, the determination of chitosan compound ratio and film amount
1st, the impact to composite membrane fungistatic effect for the film amount
Table 6 is the proportioning of three kinds of chitosan films, and as can be seen from Table 7, formula one (is coated on 10 when film amount is 3ml
On × 10cm kraft paper, similarly hereinafter) there is fungistatic effect, and inhibition zone width increases with the increase of film amount.Formula two is applying
Just there are fungistatic effect, inhibition zone width first increases and then decreases during 2ml, reach maximum in 6ml.The inhibition zone width of formula three,
Reach maximum in film amount 6ml, other is all slightly smaller.Film amount to three kinds of formula, when formula two applies 6ml shitosan mixed liquor
Fungistatic effect is best.
Table 6 Chitosan-phospholipid complex mixed liquor proportioning
The impact to film fungistatic effect for the table 7 film amount
Note：The a diameter of 6mm of recombination chitosan film that bacteriostatic experiment adopts, experimental bacteria is bacillus subtilises, similarly hereinafter
2nd, the impact to chitosan film thickness for the film amount
As seen from Figure 1 with the increase of film amount on kraft paper, the thickness of the composite membrane of three kinds of formula is all gradually
Increase, and staggered rising, the especially increase of formula three are slightly more substantially.When film amount is to bring up to 6ml from 5ml, can see
The thickness going out chitosan film all quickly increases, and its reason is likely due to kraft paper and has absorbed mixed liquor in film amount for 5ml
Saturation, if being further continued for masking liquid, kraft paper is unable to re-absorption.
3rd, the impact to composite membrane water absorption rate for the film amount
As seen from Figure 2, being gradually increased with film amount on kraft paper, the water absorption rate of three kinds of formula composite membranes is equal
Rapid increase.This is that the moisture losing after the drying is relatively more because film amount is more.Therefore composite membrane is added to the water
When, the film being dried can absorb more water, so water absorption rate and film amount assume linear approximate relationship.
Result in the above-mentioned table of Integrated comparative 7, Fig. 1 and Fig. 2, the film thickness of three kinds of formula and Water absorption values are approximate, antibacterial
Effect difference is larger, and fungistatic effect is the main performance assessment criteria of film properties, and therefore formula two effect is preferable, therefore selects formula
Two.In composite membrane, the proportioning of Chitosan-phospholipid complex is：Chitosan hydrochlorate 1.4%, molecular weight 100,000 shitosan 0.6%,
Deacetylation 95% shitosan 2%.
4th, the impact to composite membrane appearance features for the film amount
Table 8 film amount affects on the appearance features of chitosan complex film
As can be seen from Table 8, with the increase of film amount, the color of film, abnormal smells from the patient, hardness all changes.When film is used
, more than after 7ml, the surface of finished product is more harder poor with food stickiness for amount, should not be used as the antiseptic packaging films of food.
5th, the impact to composite membrane other performance for the film amount
The impact to film properties parameter for the table 9 film amount
As seen from Table 9, with the increase of film amount, the tensile break stress of composite membrane and elongation at break all gradually increase
Plus, when film amount is 8ml, composite membrane exceedes the measurement range (the maximum load power of Texture instrument is 1000N) of Texture instrument.Film
The elongation at break of amount 6ml, tensile break stress is close with film amount 7ml.Be coated on kraft paper because of shitosan mixed liquor therefore
Film amount affects less, moisture-vapor transmission minimum when film amount 5ml and 6ml to moisture-vapor transmission.
Consolidated statement 7, table 8, table 9 and Fig. 1, Fig. 2 result, with the antibacterial circle diameter of film, appearance features, tensile break stress and
Elongation at break is main performance assessment criteria, with water absorption rate, thickness and moisture-vapor transmission for secondary reference index, determines and works as film
Measure all more satisfactory for leading indicator result above-mentioned during 6ml, final choice optimal film amount is to apply 6ml on 10 × 10cm kraft paper.
4th, the determination of tween and antibacterial addition
1st, the impact to film properties for the tween addition
Tween, as emulsifying agent, can make each solute in shitosan mixed liquor dispersed, adds in chitosan compound
After tween, the performance of chitosan complex film is shown in Table 10.As can be seen from Table 10, antibacterial circle diameter with tween addition increase and
Increase, when tween addition is 1.6%, antibacterial circle diameter reaches 12.1mm, differs only 0.7mm with maximum 12.8mm.When
When tween content is 1.6%, the elongation at break of chitosan complex film is up to 6.6%, and tensile break stress is 24.5N, inhales
Water rate is 95.2%, and both of which and tween content are that 1.8% phase difference is less.When tween addition is 1.6%, vapor is saturating
Rate of crossing is minimum it can be seen that the optimal addn of tween is 1.6%.
The impact to compound film properties for table 10 tween
2nd, the impact to film fungistatic effect for the nisin addition
The nisin of polypeptide structure can suppress the growth of some microorganisms, because of its seldom therefore to film of its addition
He does not affect performance, so only investigating its impact to fungistatic effect.It is added into antibacterial to film in shitosan mixed liquor
Influential effect is shown in Table 11, and from table 11, with the increase of nisin addition, the inhibition zone of recombination chitosan film is straight
Footpath is gradually increased, and reaches peak 19.6mm during to 0.06%.When continuing to increase streptococcus acidi lactici cellulose content, antibacterial circle diameter is slightly
There is decline.So selecting addition more suitable for 0.06%.
The impact to composite membrane fungistatic effect for table 11 nisin
3rd, the impact to film fungistatic effect for the prodigiosin addition
Prodigiosin is a kind of small-molecule substance from microorganism, because it is nontoxic, bright-colored and can suppress
The characteristics such as growth of microorganism thus can be used as food additive, be added in shitosan mixed liquor, the impact of the fungistatic effect of film being shown in
Table 12, from table 12, with the increase of prodigiosin content, antibacterial circle diameter is gradually increased, and reaches highest during to 0.05%,
But 0.03～O.05% between addition antibacterial circle diameter increase inconspicuous, its fungistatic effect of identical addition does not have lactic acid chain
Coccus element is good, and in addition prodigiosin price is higher, and market is difficult to buy, and nisin wide material sources, cheap, institute
Abandon addition prodigiosin to contain.
The impact to composite membrane fungistatic effect for table 12 prodigiosin
5th, food preservation experimental result
1st, the preservation experimental result of preserved apple
Table 13 is the preservation experimental result in 37 DEG C of incubators for the preserved apple making not adding preservative agent by oneself, according to GB14884-
The total plate count of 2003 candied fruit based food sanitary standards requires as≤1000cfu/g, and matched group preserved fruit detects for second day
Microorganism, exceeded national standard to the 8th day and requires, have sugar liquid to ooze out kraft paper, and it is mouldy, in generation in the 12nd day that sugar liquid oozes out position
Go mouldy.And the preserved fruit of chitosan film parcel preserves to the 14th day detection microorganism not yet.With reference to effective period of food quality experimental calculation side
Method, in normal temperature environment, with chitosan film parcel the preserved apple shelf-life minimum can reach 42 days, chitosan film to prevent fruit
The modification effect of dried meat is fine.
The preservation experimental result to preserved fruit for table 13 composite membrane
2nd, moon cake preserves experimental result
Table 14 is the preservation experimental result in 37 DEG C of incubators for the five-kernel moon cake making not adding preservative agent by oneself, according to GB7099-
The total plate count of 2003 pastry food sanitary standards requires as≤1500cfu/g.Test sampling in the 2nd day to find, matched group bag
A large amount of oils and fatss are oozed out on the moon cake surface of dress, and chitosan complex film surface no this phenomenon, chitosan complex film was to the 4th day beginning table
Face has amount of grease to overflow, and compared with kraft paper, the oil removal ability of chitosan complex film is preferable.As shown in Table 14, the matched group moon
Cake began with microorganism and generates from the 4th day, connect beyond national standard at the 6th day.And existed with the moon cake of chitosan complex film parcel
Do not detect microorganism yet within 14th day.With reference to effective period of food quality experimental calculation method, in normal temperature environment, with chitosan film parcel
Moon Cake Quality Guarantee is minimum to can reach 42 days.The Moon Cake Quality Guarantee adding preservative in the market requires, for 30-60 days, therefore to exist
Chitosan complex film has good application prospect in terms of moon cake packaging.
The preservation experimental result to moon cake for table 14 composite membrane
The assay method of film properties
(1) measurement of thickness：Randomly choose 10 point surveying records of film with micrometer caliper, take its meansigma methods.
(2) mensure of film water absorption rate：Chitosan film is cut into the sample of 2cm × 2cm, is dried to constant weight in drying baker,
Now weight is designated as W1.Dry film is put in distilled water, under the conditions of 37 DEG C, constant temperature preserves 24h.With filter paper by film surface after taking-up
Water blots, and weighs quality, is designated as W2, calculates water absorption rate.
(3) mensure of film water vapor transmission rates：According to GB, using plan agar diffusion method.
(4) mensure of film dynamic performance：Take the thin film of the defects such as the uniform, bubble-free of thickness, be cut into the strip of 5cm × 1cm.
Survey tensile break stress and elongation at break with Texture instrument.During measurement, the distance between fixture is 10mm, carrying 1000N, speed
For 35mm/min.
(5) mensure of film antibacterial circle diameter：Film puncher is broken into the sequin of diameter 6mm, be placed in and go out under uviol lamp
Bacterium 30min.The bacteria suspension drawing bacteria suspension 0.1ml suitable concentration coats bacteria culture media flat board, with sterilizing tweezers gripping sample
Product, are attached to standing laminating in culture medium, cultivate 24h, surveying record antibacterial circle diameter at 37 DEG C.
Compared with prior art, the present invention is by the shitosan of the deacetylation 95% of different fungistatic effects, molecular weight 100,000
Shitosan and chitosan hydrochlorate make mixed liquor, mixed liquor adds emulsifier tween and antibacterial streptococcus acidi lactici
Element, mixed liquor is coated on kraft paper and makes tensile break stress and elongation at break is higher, fungistatic effect is reasonable
Chitosan film.This film can be used as the pastry foods such as moon cake and the packaging film of preserved fruit based food, packs after this based food with this film,
It is not required in food add the shelf life requirements that preservative can reach national regulation.The low production cost of this film, can reach edible
Standard, has no effect to food quality, and application surface is extensive.
Brief description
Fig. 1 is the impact figure to film thickness for the film amount；
Fig. 2 is the impact figure to film water absorption rate for the film amount.
Specific embodiment
The concrete technical scheme of the present invention described further below, is further understood that in order to those skilled in the art
The present invention, and do not constitute the restriction to its right.
Embodiment 1, a kind of preparation method of the antibacterial food wrapper of recombination chitosan, comprise the steps：
(1) shitosan of deacetylation 95% is dissolved in the acetic acid solution of 1% volumetric concentration, is heated to 50 DEG C of stirrings
To uniformly not having granule to obtain solution A, it is configured to the shitosan acetic acid solution of mass fraction 4～6%；
(2) shitosan of molecular weight 100,000 is placed in the acetic acid solution of 1% volumetric concentration, is heated to 50 DEG C and stirs to equal
Even solution B, is configured to mass fraction 0.9～6.3% shitosan acetic acid solution；
(3) chitosan hydrochlorate is placed in the acetic acid solution of 1% volumetric concentration, stirs to dissolving to obtain solution C, prepare dense
Degree 0.9～6.3% shitosan acetic acid solution；
(4) ratio of solution A, solution B and solution C in mass ratio 1: 1: 1 is mixed, add tween and streptococcus acidi lactici
Element, makes tween mass concentration in mixed liquor be 1.0～1.8%, the mass concentration of nisin is：0.02～0.1%, will
Mixed liquor stirs, and only puts 12h degassing, mixed liquor is spread evenly across on kraft paper, using multiple film, first few rear many,
The mode of repeated drying, until mixed liquor is painted with completely, dries 2.5h film forming at 55 DEG C.
Embodiment 2, in the preparation method described in embodiment 1：The mass fraction making solution A in mixed liquor is 2%, solution B
Mass fraction be 0.6%, the mass fraction of solution C is 1.4%.Remaining is same as Example 1.
Embodiment 3, in the preparation method described in embodiment 1 or 2：The number of times of film at least 5 times, is once dried using often applying
Dry mode once.
Embodiment 4, in the preparation method described in embodiment 1 or 2 or 3：Tween in mixed solution described in step (4)
Mass concentration is 1.6%.In described mixed solution, the mass concentration of nisin is 0.06%.
Embodiment 5, in the preparation method described in embodiment 1-4 any one：Described film amount is：Every 10cm ×
5～7mL mixed liquor is applied on the kraft paper of 10cm.
Embodiment 6, a kind of preparation method of the antibacterial food wrapper of recombination chitosan：Shitosan by deacetylation 95%
It is placed in the acetic acid solution of 1% (v/v), be heated to 50 DEG C and be stirred until homogeneous do not have granule to obtain solution A, being configured to mass fraction is
6% shitosan acetic acid solution；The shitosan of molecular weight 100,000 is placed in the acetic acid solution of 1% (v/v), is heated to 50 DEG C and stirs
Mix to dissolving to obtain solution B, be configured to the shitosan acetic acid solution of the molecular weight 100,000 of mass fraction 1.8%；By shitosan hydrochloric acid
Salt is dissolved in the acetic acid solution of 1% (v/v), stirs to dissolving to obtain solution C, is configured to the shitosan hydrochloric acid that concentration is 4.2%
Salt acetic acid solution.By solution A, solution B and solution C with 32.8g: 32.8g: 32.8g ratio mixing and stirring becomes shitosan
Mixed liquor, adds 1.6g tween, 0.06g nisin in mixed liquor, mixed solution is stirred, and only puts 12h and takes off
Gas, takes 6ml shitosan mixed liquor to be spread evenly across on the kraft paper of 10 × 10cm, using multiple film, first few rear many, repeatedly dries
Dry mode, until mixed liquor is painted with completely, dries 2.5h film forming at 55 DEG C.
The antibacterial food wrapper of recombination chitosan is applied：Antibacterial for recombination chitosan food wrapper is wrapped in not adding preservative agent
Preserved apple on as packing inside bag, outer package adopts general composite plastic package bag, and outer packaging bag is sealed after normal
The lower preservation of temperature was never degenerated at least 42 days, and the sanitary standard of this preserved fruit meets GB GB14884-2003 and requires.
Embodiment 7, a kind of preparation method of the antibacterial food wrapper of recombination chitosan：Shitosan by deacetylation 95%
It is placed in the acetic acid solution of 1% (v/v), be heated to 50 DEG C and be stirred until homogeneous do not have granule to obtain solution A, being configured to mass fraction is
6% shitosan acetic acid solution；The shitosan of molecular weight 100,000 is placed in the acetic acid solution of 1% (v/v), is heated to 50 DEG C and stirs
Mix to dissolving to obtain solution B, be configured to the shitosan acetic acid solution of the molecular weight 100,000 of mass fraction 1.8%；By shitosan hydrochloric acid
Salt is dissolved in the acetic acid solution of 1% (v/v), stirs to dissolving to obtain solution C, is configured to the shitosan hydrochloric acid that concentration is 4.2%
Salt acetic acid solution.By solution A, solution B and solution C with 32.7g: 32.7g: 32.7g ratio mixing and stirring becomes shitosan
Mixed liquor, adds 1.8g tween, 0.04g nisin in mixed liquor, mixed solution is stirred, and only puts 12h and takes off
Gas, takes 5ml shitosan mixed liquor to be spread evenly across on the kraft paper of 10 × 10cm, using multiple film, first few rear many, repeatedly dries
Dry mode, until mixed liquor is painted with completely, dries 2.5h film forming at 55 DEG C.
The antibacterial food wrapper of recombination chitosan is applied：Antibacterial for recombination chitosan food wrapper is wrapped in be not added with anti-corrosion
As packing inside bag on the five-kernel moon cake of agent, outer package adopts general composite plastic package bag, by outer packaging bag sealing after
At least can preserve under room temperature and never degenerate to 42 days, the sanitary standard of this moon cake meets GB GB7099-2003 and requires.
Claims (7)
1. a kind of antibacterial food wrapper of recombination chitosan preparation method it is characterised in that：The method comprises the steps：
(1) shitosan of deacetylation 95% is dissolved in the acetic acid solution of 1% volumetric concentration, be heated to 50 DEG C stir to
Uniformly do not have granule to obtain solution A, be configured to the shitosan acetic acid solution of mass fraction 4 ~ 6%；
(2) shitosan of molecular weight 100,000 is placed in the acetic acid solution of 1% volumetric concentration, is heated to 50 DEG C and is stirred until homogeneous
Obtain solution B, be configured to mass fraction 0.9 ~ 6.3% shitosan acetic acid solution；
(3) chitosan hydrochlorate is placed in the acetic acid solution of 1% volumetric concentration, stirs to dissolving to obtain solution C, compound concentration
0.9 ~ 6.3% shitosan acetic acid solution；
(4) by solution A, solution B and solution C in mass ratio 1:1:1 ratio mixing, adds tween and nisin, makes
In mixed liquor, tween mass concentration is 1.0 ~ 1.8%, and the mass concentration of nisin is：0.02 ~ 0.1%, mixed liquor is stirred
Mix uniformly, only put 12h degassing, mixed liquor is spread evenly across on kraft paper, using multiple film, first few rear many, repeated drying
Mode, until mixed liquor is painted with completely, at 55 DEG C, dry 2.5h film forming.
2. preparation method according to claim 1 it is characterised in that：The mass fraction making solution A in mixed liquor is 2%, molten
The mass fraction of liquid B is 0.6%, and the mass fraction of solution C is 1.4%.
3. preparation method according to claim 1 it is characterised in that：The number of times of film at least 5 times, using often applying once
Dry mode once.
4. preparation method according to claim 1 it is characterised in that：Tween in mixed solution described in step (4)
Mass concentration is 1.6%.
5. preparation method according to claim 1 it is characterised in that：Lactic acid chain in mixed solution described in step (4)
The mass concentration of coccus element is 0.06%.
6. preparation method according to claim 1 it is characterised in that：Film amount described in step (4) is：In every 10cm
5 ~ 7mL mixed liquor is applied on the kraft paper of × 10cm.
7. the method described in claim 1 be obtained the antibacterial food wrapper of recombination chitosan application it is characterised in that：Described
The antibacterial food wrapper of recombination chitosan be used for the packaging of moon cake or preserved apple.
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| CN108999023A (en) * | 2018-07-28 | 2018-12-14 | 袁杰 | A kind of greaseproof and preparation method thereof |
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