CN106434818A - Fermentation medium for synthesizing riboflavin by bacillus subtilis - Google Patents
Fermentation medium for synthesizing riboflavin by bacillus subtilis Download PDFInfo
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- CN106434818A CN106434818A CN201611194898.6A CN201611194898A CN106434818A CN 106434818 A CN106434818 A CN 106434818A CN 201611194898 A CN201611194898 A CN 201611194898A CN 106434818 A CN106434818 A CN 106434818A
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- riboflavin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
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- General Engineering & Computer Science (AREA)
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Abstract
The invention discloses a fermentation medium for synthesizing riboflavin by bacillus subtilis. The fermentation medium comprises, by weight, 80 parts of glucose, 0.5-1.5 parts of dipotassium phosphate, 0.5-1.5 parts of monopotassium phosphate, 0.5-1.5 parts of magnesium sulfate heptahydrate, 3-5 parts of ammonium sulfate, 3-5 parts of sodium nitrate, 20-25 parts of yeast powder and 5-15 parts of cottonseed protein. By optimizing nitrogen source composition in the fermentation medium, the improved fermentation medium is designed and can bring a positive effect on the fermentation process of synthesizing the riboflavin by the bacillus subtilis, and the yield of the riboflavin is improved by 20-25%.
Description
Technical field
The invention belongs to biomedicine technical field, more particularly to a kind of utilization bacillus subtilises riboflavin biosynthesis
Fermentation medium.
Background technology
Conventional riboflavin commodity production mainly adopts chemical synthesising technology route, and at present, many manufacturers begin with
Production by Microorganism Fermentation riboflavin.Compared with chemical synthesiss, fermentable low cost, pollution is few, therefore suffers from the world
The favor of riboflavin manufacturer.Nitrogen source is one of major influence factors of fermentable, by can be right to the optimization of nitrogen source
Sweat produces active influence.It is thus desirable to providing a kind of promotion growth, the fermentation medium of raising fermentation yield.
Content of the invention
The purpose of the present invention is that the yield improving using bacillus subtilises riboflavin biosynthesis.
For reaching above-mentioned purpose, the present invention is to implement according to technical scheme below：
A kind of fermentation medium of utilization bacillus subtilises riboflavin biosynthesis, comprises the composition of following parts by weight：80 parts
Glucose, the dipotassium hydrogen phosphate of 0.5-1.5 part, the potassium dihydrogen phosphate of 0.5-1.5 part, the bitter salt of 0.5-1.5 part,
The ammonium sulfate of 3-5 part, the cottonseed protein of the sodium nitrate of 3-5 part, the yeast powder of 20-25 part and 5-15 part.
Further, described fermentation medium comprises the composition of following parts by weight：80 parts of glucose, 1 part of phosphorus
Sour hydrogen dipotassium, 1 part of potassium dihydrogen phosphate, 1 part of bitter salt, 5 parts of ammonium sulfate, 5 parts of sodium nitrate, 20 parts of yeast
The cottonseed protein of powder and 5 parts.
Compared with prior art, beneficial effects of the present invention are：
The present invention, by being optimized to the nitrogen source composition in fermentation medium, devises a kind of fermentation medium of improvement, should
Culture medium can produce active influence to the sweat using bacillus subtilises riboflavin biosynthesis, by the yield of riboflavin
Improve 20-25%.
Specific embodiment
With reference to specific embodiment, the invention will be further described, illustrative examples and explanation that here is invented
It is used for explaining the present invention, but not as a limitation of the invention.
Embodiment 1
A kind of fermentation medium of utilization bacillus subtilises riboflavin biosynthesis, comprises the composition of following parts by weight：80 parts
Glucose, 1.5 parts of dipotassium hydrogen phosphate, 1.5 parts of potassium dihydrogen phosphate, 0.5 part of bitter salt, 5 parts of ammonium sulfate,
The cottonseed protein of 5 parts of sodium nitrate, 20 parts of yeast powder and 10 parts.
Embodiment 2
A kind of fermentation medium of utilization bacillus subtilises riboflavin biosynthesis, comprises the composition of following parts by weight：80 parts
Glucose, 1.5 parts of dipotassium hydrogen phosphate, 1.5 parts of potassium dihydrogen phosphate, 0.5 part of bitter salt, 3 parts of ammonium sulfate,
The cottonseed protein of 3 parts of sodium nitrate, 25 parts of yeast powder and 5 parts.
Embodiment 3
A kind of fermentation medium of utilization bacillus subtilises riboflavin biosynthesis, comprises the composition of following parts by weight：80 parts
Glucose, 1 part of dipotassium hydrogen phosphate, 1 part of potassium dihydrogen phosphate, 1 part of bitter salt, 5 parts of ammonium sulfate, 5 parts
The cottonseed protein of sodium nitrate, 20 parts of yeast powder and 5 parts.
Technical scheme is not limited to the restriction of above-mentioned specific embodiment, and every technology according to the present invention scheme is done
The technology deformation going out, each falls within protection scope of the present invention.
Claims (2)
1. a kind of fermentation medium using bacillus subtilises riboflavin biosynthesis is it is characterised in that comprise following weight portion
The composition of number：80 parts of glucose, the dipotassium hydrogen phosphate of 0.5-1.5 part, the potassium dihydrogen phosphate of 0.5-1.5 part, 0.5-1.5 part
Bitter salt, the ammonium sulfate of 3-5 part, the cottonseed protein of the sodium nitrate of 3-5 part, the yeast powder of 20-25 part and 5-15 part.
2. the fermentation medium of a kind of utilization bacillus subtilises riboflavin biosynthesis according to claim 1, its feature exists
In comprising the composition of following parts by weight：80 parts of glucose, 1 part of dipotassium hydrogen phosphate, 1 part of potassium dihydrogen phosphate, 1 part
Bitter salt, 5 parts of ammonium sulfate, 5 parts of sodium nitrate, the cottonseed protein of 20 parts of yeast powder and 5 parts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611194898.6A CN106434818A (en) | 2016-12-22 | 2016-12-22 | Fermentation medium for synthesizing riboflavin by bacillus subtilis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611194898.6A CN106434818A (en) | 2016-12-22 | 2016-12-22 | Fermentation medium for synthesizing riboflavin by bacillus subtilis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106434818A true CN106434818A (en) | 2017-02-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201611194898.6A Pending CN106434818A (en) | 2016-12-22 | 2016-12-22 | Fermentation medium for synthesizing riboflavin by bacillus subtilis |
Country Status (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109609580A (en) * | 2018-12-26 | 2019-04-12 | 河南巨龙生物工程股份有限公司 | A kind of fermentation medium and its fermentation process of riboflavin |
| CN110777184A (en) * | 2019-11-27 | 2020-02-11 | 河北圣雪大成制药有限责任公司 | Fermentation medium for fermenting riboflavin and application method thereof |
| CN112980715A (en) * | 2020-12-14 | 2021-06-18 | 内蒙古工业大学 | Bacillus subtilis strain B13 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914478A (en) * | 2010-08-06 | 2010-12-15 | 新发药业有限公司 | Bacillus subtilis and application thereof |
| CN102154426A (en) * | 2010-12-28 | 2011-08-17 | 广济药业（孟州）有限公司 | Industrial fermentation method of riboflavin |
| CN103695519A (en) * | 2013-12-23 | 2014-04-02 | 广济药业（孟州）有限公司 | Nitrogen source, culture medium and culture method for producing riboflavin |
-
2016
- 2016-12-22 CN CN201611194898.6A patent/CN106434818A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914478A (en) * | 2010-08-06 | 2010-12-15 | 新发药业有限公司 | Bacillus subtilis and application thereof |
| CN102154426A (en) * | 2010-12-28 | 2011-08-17 | 广济药业（孟州）有限公司 | Industrial fermentation method of riboflavin |
| CN103695519A (en) * | 2013-12-23 | 2014-04-02 | 广济药业（孟州）有限公司 | Nitrogen source, culture medium and culture method for producing riboflavin |
Non-Patent Citations (3)
| Title |
|---|
| ZHIWEN WANG ET AL.: "Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum", 《BIORESOURCE TECHNOLOGY》 * |
| 夏苗苗等: "枯草芽孢杆菌核黄素操纵子与ribC基因的修饰与遗传效应", 《微生物学通报》 * |
| 石悦: "枯草芽孢杆菌24A1/pMX45核黄素发酵工艺的研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109609580A (en) * | 2018-12-26 | 2019-04-12 | 河南巨龙生物工程股份有限公司 | A kind of fermentation medium and its fermentation process of riboflavin |
| CN109609580B (en) * | 2018-12-26 | 2022-04-01 | 河南巨龙生物工程股份有限公司 | Fermentation medium and fermentation method of riboflavin |
| CN110777184A (en) * | 2019-11-27 | 2020-02-11 | 河北圣雪大成制药有限责任公司 | Fermentation medium for fermenting riboflavin and application method thereof |
| CN110777184B (en) * | 2019-11-27 | 2023-03-03 | 河北圣雪大成制药有限责任公司 | Fermentation medium for fermenting riboflavin and application method thereof |
| CN112980715A (en) * | 2020-12-14 | 2021-06-18 | 内蒙古工业大学 | Bacillus subtilis strain B13 and application thereof |
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