CN109837322A - A kind of simple fermentation medium using bacillus subtilis production vitamin B2 - Google Patents
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 Download PDFInfo
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- CN109837322A CN109837322A CN201910238642.8A CN201910238642A CN109837322A CN 109837322 A CN109837322 A CN 109837322A CN 201910238642 A CN201910238642 A CN 201910238642A CN 109837322 A CN109837322 A CN 109837322A
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- bacillus subtilis
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Abstract
The present invention relates to technical field of bioengineering, especially a kind of simple fermentation medium using bacillus subtilis production vitamin B2.This kind includes: urea, yeast extract, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate using the simple fermentation medium of bacillus subtilis production vitamin B2, each component percentage are as follows: urea 0.3-1.0%, yeast extract 1-3%, sodium chloride 0.2-1.0%, sucrose 5-20%, magnesium sulfate 0.05-0.5%, ammonium sulfate 0.1-0.5%.A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of the invention, by being optimized to carbon source, the nitrogen source in fermentation medium, devise a kind of easy fermentation medium, the culture medium can shorten the ability in vitamin B2 fermentation period and fast verification bacillus subtilis synthesis vitamin B2, greatly simplifie the process of breeding high-yield vitamin B2 strain.
Description
Technical field
The present invention relates to technical field of bioengineering, especially a kind of letter using bacillus subtilis production vitamin B2
Culture medium easy to ferment.
Background technique
Vitamin B2, is one of 13 kinds of vitamin necessary to body, and vitamin B2 has extensive biological effect.Mesh
Before, the vitamin B2 production method industrially applied mainly has chemical synthesis, molecular design method and microbe fermentation method three
Kind.Compared with chemical synthesis, microbe fermentation method have simple production process, raw material is cheap and environmental pollution is few, green
The advantages that color is renewable.Therefore, most of enterprise uses microbe fermentation method at present, and bacillus subtilis is widely used in dimension
In the fermentation of raw element B2, the chemical synthesis based on petroleum is gradually being replaced.
Current fermentation medium complicated components, fermentation period is long, and riboflavin production is lower.Carbon source, nitrogen source are microorganisms
Two big important factor in order of fermentation, bacterial activity are therefore the factor of the most critical of influence vitamin B2 synthesis needs to lead to again
It crosses and provides a kind of simple fermentation medium for synthesizing vitamin B2 to the optimization of carbon source nitrogen source, shorten quick while fermentation period
Verify the ability of bacillus subtilis synthesis vitamin B2.
Summary of the invention
The purpose of the present invention is to provide it is a kind of using bacillus subtilis production vitamin B2 simple fermentation medium,
The ability for shortening fermentation period and fast verification bacillus subtilis synthesis vitamin B2, greatly simplifies breeding high-yield vitamin
The process of the bacillus subtilis strain of B2.
The technical scheme adopted by the invention to solve the technical problem is that:
A kind of simple fermentation medium using bacillus subtilis production vitamin B2, the culture medium includes: urea, yeast
Soak powder, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate.
Preferably, each component percentage are as follows: urea 0.3-1.0%, yeast extract 1-3%, sodium chloride
0.2-1.0%, sucrose 5-20%, magnesium sulfate 0.05-0.5%, ammonium sulfate 0.1-0.5%.
Preferably, the urea 0.4-0.7%, yeast extract 2-2.5%, sodium chloride 0.4-0.5%, sucrose 10-15%, sulfuric acid
Magnesium 0.15-0.3%, ammonium sulfate 0.2-0.3%.
Preferably, each component percentage are as follows: urea 0.5%, yeast extract 2%, sodium chloride 0.5%, sucrose
10%, magnesium sulfate 0.15%, ammonium sulfate 0.2%.
The present invention also provides it is a kind of using bacillus subtilis production vitamin B2 simple fermentation medium be used for into
The purposes of row fermentation of bacillus subtilis.
The beneficial effects of the present invention are: compared with prior art, a kind of produced using bacillus subtilis of the invention is tieed up
The simple fermentation medium of raw element B2 is devised a kind of easy by optimizing to carbon source, the nitrogen source in fermentation medium
Fermentation medium, the culture medium can shorten vitamin B2 fermentation period and fast verification bacillus subtilis synthesis vitamin B2
Ability, greatly simplifie the process of breeding high-yield vitamin B2 strain.
Specific embodiment
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of embodiment 1
A kind of simple fermentation medium using bacillus subtilis production vitamin B2, the culture medium includes: urea, yeast
Soak powder, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate.The each component percentage are as follows: urea 0.3%, yeast extract
1%, sodium chloride 0.2%, sucrose 5%, magnesium sulfate 0.05%, ammonium sulfate 0.1%.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of the present embodiment is withered for carrying out
When the purposes of careless fermentation of bacillus, fermentation time 72h, riboflavin concentration is about 3000mg/L in fermentation liquid.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of embodiment 2
A kind of simple fermentation medium using bacillus subtilis production vitamin B2, the culture medium includes: urea, yeast
Soak powder, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate.The each component percentage are as follows: urea 1.0%, yeast extract
3%, sodium chloride 1.0%, sucrose 20%, magnesium sulfate 0.5%, ammonium sulfate 0.5%.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of the present embodiment is withered for carrying out
When the purposes of careless fermentation of bacillus, fermentation time 72h, riboflavin concentration is about 3400mg/L in fermentation liquid.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of embodiment 3
A kind of simple fermentation medium using bacillus subtilis production vitamin B2, the culture medium includes: urea, yeast
Soak powder, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate.The each component percentage are as follows: urea 0.7%, yeast extract
2.5%, sodium chloride 0.5%, sucrose 15%, magnesium sulfate 0.15%, ammonium sulfate 0.3%.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of the present embodiment is withered for carrying out
When the purposes of careless fermentation of bacillus, fermentation time 72h, riboflavin concentration is about 4300mg/L in fermentation liquid.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of embodiment 4
A kind of simple fermentation medium using bacillus subtilis production vitamin B2, the culture medium includes: urea, yeast
Soak powder, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate.The each component percentage are as follows: urea 0.4%, yeast extract
2.0%, sodium chloride 0.4%, sucrose 15%, magnesium sulfate 0.3%, ammonium sulfate 0.3%.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of the present embodiment is withered for carrying out
When the purposes of careless fermentation of bacillus, fermentation time 72h, riboflavin concentration is about 4600mg/L in fermentation liquid.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of embodiment 5
A kind of simple fermentation medium using bacillus subtilis production vitamin B2, the culture medium includes: urea, yeast
Soak powder, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate.The each component percentage are as follows: urea 0.5%, yeast extract
2%, sodium chloride 0.5%, sucrose 10%, magnesium sulfate 0.15%, ammonium sulfate 0.2%.
A kind of simple fermentation medium using bacillus subtilis production vitamin B2 of the present embodiment is withered for carrying out
When the purposes of careless fermentation of bacillus, fermentation time 72h, riboflavin concentration is about 4900mg/L in fermentation liquid.
Above-mentioned specific embodiment is only specific case of the invention, and scope of patent protection of the invention includes but is not limited to
The product form and style of above-mentioned specific embodiment, any claims of the present invention and any technical field of meeting
The appropriate changes or modifications that those of ordinary skill does it, all shall fall within the protection scope of the present invention.
Claims (5)
1. a kind of simple fermentation medium using bacillus subtilis production vitamin B2, it is characterised in that: the culture medium
It include: urea, yeast extract, sodium chloride, sucrose, magnesium sulfate and ammonium sulfate.
2. a kind of simple fermentation medium using bacillus subtilis production vitamin B2 according to claim 1,
It is characterized in that: each component percentage are as follows: urea 0.3-1.0%, yeast extract 1-3%, sodium chloride 0.2-1.0%,
Sucrose 5-20%, magnesium sulfate 0.05-0.5%, ammonium sulfate 0.1-0.5%.
3. a kind of simple fermentation medium using bacillus subtilis production vitamin B2 according to claim 1,
It is characterized in that: each component percentage are as follows: urea 0.4-0.7%, yeast extract 2-2.5%, sodium chloride 0.4-
0.5%, sucrose 10-15%, magnesium sulfate 0.15-0.3%, ammonium sulfate 0.2-0.3%.
4. a kind of simple fermentation medium using bacillus subtilis production vitamin B2 according to claim 1,
It is characterized in that: each component percentage are as follows: urea 0.5%, yeast extract 2%, sodium chloride 0.5%, sucrose 10%, sulphur
Sour magnesium 0.15%, ammonium sulfate 0.2%.
5. being used to carry out the purposes of fermentation of bacillus subtilis according to the simple fermentation medium of any one of claim 2-4.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110241166A (en) * | 2019-06-20 | 2019-09-17 | 山东泓达生物科技有限公司 | It is a kind of promote riboflavin fermentation composition and its application |
| CN110564804A (en) * | 2019-09-12 | 2019-12-13 | 河南巨龙生物工程股份有限公司 | Clear liquid fermentation medium for producing riboflavin and fermentation method |
| CN115216419A (en) * | 2021-04-21 | 2022-10-21 | 宁夏金维制药股份有限公司 | Culture medium for producing vitamin B2 by fermenting bacillus subtilis engineering bacteria |
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| CN110564804A (en) * | 2019-09-12 | 2019-12-13 | 河南巨龙生物工程股份有限公司 | Clear liquid fermentation medium for producing riboflavin and fermentation method |
| CN115216419A (en) * | 2021-04-21 | 2022-10-21 | 宁夏金维制药股份有限公司 | Culture medium for producing vitamin B2 by fermenting bacillus subtilis engineering bacteria |
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