CN106434343A - Remote biological cell culture apparatus, auxiliary system and application of system - Google Patents
Remote biological cell culture apparatus, auxiliary system and application of system Download PDFInfo
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- CN106434343A CN106434343A CN201610865859.8A CN201610865859A CN106434343A CN 106434343 A CN106434343 A CN 106434343A CN 201610865859 A CN201610865859 A CN 201610865859A CN 106434343 A CN106434343 A CN 106434343A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/28—Means for regulation, monitoring, measurement or control, e.g. flow regulation of redox potential
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B19/00—Programme-control systems
- G05B19/02—Programme-control systems electric
- G05B19/04—Programme control other than numerical control, i.e. in sequence controllers or logic controllers
- G05B19/042—Programme control other than numerical control, i.e. in sequence controllers or logic controllers using digital processors
Abstract
The invention provides a remote biological cell culture apparatus and an auxiliary system and application of the system, relates to an auxiliary system for remotely monitoring and early warning biological cell growth environment and application thereof and aims at overcoming the defects that at present, an existing biological culture box is only limited to temperature and humidity control, cannot conduct net acquisition and detailed graph analysis on data by itself and also does not have the functions of real-time transmission, monitoring and early warning of biological cells. A silver ion partition plate, a gas concentration sensor, a water level sensor, a temperature and humidity sensor, an oxygen sensor and a conductivity sensor are arranged in the cavity of a box body, and connection between the system and terminal monitoring early warning software is controlled through a single-board computer long-distance switch to achieve remote control over the biological cell culture process. The system is used for culturing cells or viruses from mammals, microbes and plant cells.
Description
Technical field
The present invention relates to a kind of biological cell culture apparatuses and aid system and its application, more particularly to a kind of remote monitoring
Early warning Growth of Biologic Cell environment aid system and its application.
Background technology
The device can be widely applied to each scientific research, clinic and medical institutions at present, for cell, tissue culture and some spies
The culture of different microorganism, cyto-dynamics research, the collection of mammalian cell secretions, various physics, the cause of chemical factor
Cancer or toxicological effect, the research of antigen and production, culture hybridoma production antibody, in vitro fertilization (IVF), stem cell, group
The research fields such as weaver's journey, drug screening.The quasi-instrument because its pollution be cause cell culture failure a principal element, greatly
Require more and manage in strict confined space in GMP, and apply in the high environment of cleanliness class.Existing biological cell culture
Case is only limitted to control temperature and gas concentration, and itself data can not be carried out with network collection and detailed graphic analyses, similar
Product is only capable of by additionally supporting outer sensor, is carried out local transmissions, and is adopted electromagnetic wave FM signal local transmissions, signal
Coverage effect is poor, and is only capable of by phone, message form early warning, it is impossible to reach the effect of monitor in real time, in addition a lot of sample itself
Originally can only once obtain, because of the sample losses that the device causes, will be unable to retrieve.Therefore, it is pre- how such instruments monitor is improved
Alert promptness, real-time, while improving stability and convenient promptness, the safety to cell culture, are the technology of this area
Personnel have problem to be solved, are finally reached closest to preferably high simulation microorganism culture environment, so as to the good of the industry
Sexual development plays positive innovation progradations.
Content of the invention
It is an object of the invention to solve existing bio-incubator at present to be only limitted to control humiture, can not itself be right
Data carry out network collection and detailed graphic analyses, and also do not possess carries out real-time Transmission monitoring and early warning function to biological cell
Deficiency, and a kind of biological cell culture for providing expands monitoring and early warning aid system and its application.
A kind of remote biometric cell culture system of the present invention and aid system, it include casing, gas concentration sensor,
Single board computer computer remote on-off control system, silver ion dividing plate, water level sensor, control panel, measuring device fan, humiture
Sensor, balance pipe, cabinet door, oxygen sensor and conductivity sensor；
Cavity is provided with the casing, cavity medial wall top is provided with measuring device fan, in measuring device fan week
The cavity medial wall top that encloses is provided with gas concentration sensor, Temperature Humidity Sensor and oxygen sensor around measuring device fan；
Described cavity medial wall bottom is provided with water level sensor and conductivity sensor；Be provided with described cavity multiple silver from
Sub-dividing plate；On described cavity medial wall, rear portion is provided with balance pipe；
Described cabinet front is provided with cabinet door, and the cavity is closed by cabinet door；
Described control panel is arranged at the top of cabinet front, and is located above cabinet door；
Described single board computer computer remote on-off control system is arranged on the lateral wall on the right side of casing；
Described Temperature Humidity Sensor, gas concentration sensor, the signal output of conductivity sensor and water level sensor
End is connected with a signal receiving end of single board computer computer remote on-off control system respectively；The command signal of control panel is defeated
Go out end to be connected with another signal receiving end of single board computer computer remote on-off control system.
The balance pipe of the present invention is that cavity is in communication with the outside, and plays a part of balance to pressure in cavity etc..
A kind of remote biometric cell culture system of the present invention and aid system are used for culture from the thin of mammal
Born of the same parents, virus, microorganism and plant cell.
The present invention passes through its parameter feedback by arranging conductivity sensor, can microorganism life in the pure water of effective monitoring bottom
Long status, by, in suspension culture microbial physiology, the research of biochemical characteristic, finding growth of microorganism and peroxidase activity
Property be in parallel relation, with electrical conductivity be in contrary mirror image relationship.It is possible thereby to by microorganism in pure water in different shears
Conductance under rate is flowed and (f=0.2MHz) pure electrical conductivity of water and electric capacity under resting state to measure respectively, finds microorganism
Electrical conductivity change with microbial morphology, can by quantifying germs collect, adherent sexual abnormality is come microorganisms growth conditions
Situation, by measuring the electric rotational spectrum of microorganism using electrorotation chip, and then detects that the electricity in microorganism growth process is special
Property change rule, as a result show the increase with the time, the electric capacity of the film of microorganism is gradually reduced, and determines on this basis thin
Bacterium, the conductance method of fungal metabolite are detecting growth of microorganism state.When their activity is relatively low, electrical conductivity is with activity
Increase and increase, the index be used for represent that growth of microorganism state has obvious indicative function, the setting electrical conductivity of the present invention
Sensor measurement range reaches：0.001 μ S/cm to 300mS/cm, resolution：0.001 μ S of minima, precision：Parameter>3μS
For 0.5% ± 1；Parameter≤3 μ S is 0.5% ± 0.01 μ S.
Described single board computer computer remote on-off control system comprising remote switch control system, single board computer computer,
With sensor internet platform.
Described single board computer computer remote on-off control system is equipped with brand-new ARM architecture processor, 10/100Mbps net
Mouth, built-in Ethernet, a com interface and a RS485 Interface Terminal simultaneously or independently can distribute, and support TCP
Server, TCP Client, UDP several working ways, baud rate supports 600bps～230.4Kbps, back-level server domain name
Address resolution.
Operating system supported web page is self-defined, disclosure satisfy that basic network browsing, data processing and computer programming
Needs.
Single board computer computer remote on-off control system can be with place remote control device switching on and shutting down, remotely located timing control
System, remote emergency starts the functions such as UPS, reaches accurate convenient manipulation.
Sensor internet platform can carry out data lossless biography by TCP Server, TCP Client isotype
Defeated, by the high in the clouds platform of TCP Client public network server or TCP Server, local data is realized cross-network segment transmission, with
Just real time data is seen on other places are by the Internet whenever and wherever possible, while extract with same software showing pictorialization text
Part, can more intuitively be analyzed monitoring.
The single board computer computer remote on-off control system of the present invention, remote control function flow process：Calculated by single board computer
The sensor analog signals data of machine collection, are converted to digital signal data through parsing, and are stored and processed, while passing
Asynchronous transmission standard interface RS232, RS485 interface and the network port is transported to, externally timing sends number via single board computer computer
Word signal data bag, the packet for further culture apparatuses being sent, passed through by the culture apparatuses signal data in LAN
Internet platform is sent to public network server, installs V-COM2.10 programmable software by PC terminal further, logs in synchronous
The Internet passes data, shows real time data and remote switch device by definable chart, both can achieve strange land and has transmitted to terminal
System is managed and remotely control culture apparatuses, also technique can be used for such device existing and only have RS232 communication terminal
Mouthful, by the local data that upload to public network server, in strange land, destination end management data, solve the existing at present device and are both needed to
The purpose of high cost monitoring is carried out using outer sensor.
The present invention is had the advantages that compared with prior art：
Multifunctional remote biological cell culture systems simple structure of the present invention, is easier to antibacterial copper using more current technology
Matter layer cavity and silver ion porous barrier, in conjunction with the Temperature Humidity Sensor of culture apparatuses itself configuration, gas concentration sensor, electricity
Conductivity sensor, level sensor are simultaneously based on single board computer server for core, its data in incubator can be carried out net
Network gather, especially conductivity sensor pass through its parameter feedback, can effective monitoring bottom pure water growth of microorganism state, pass through
In suspension culture microbial physiology, the research of biochemical characteristic, find growth of microorganism with peroxidase activity in parallel
Relation, is in contrary mirror image relationship with electrical conductivity.It is possible thereby to by the conductance to microorganism in pure water under different shear rates
Come measure respectively flowing and resting state under (f=0.2MHz) pure electrical conductivity of water and electric capacity, find microorganism electrical conductivity with
Microbial morphology changes, can be by quantifying germs collect, adherent sexual abnormality come microorganisms growth conditions situation, by adopting
The rule of the electrical property change in the electric rotational spectrum of microorganism, and then detection microorganism growth process are measured with electrorotation chip
Rule, as a result shows the increase with the time, and the electric capacity of the film of microorganism is gradually reduced, and determines antibacterial, fungal metabolite on this basis
The conductance method of thing is detecting growth of microorganism state.When their activity is relatively low, electrical conductivity increases with the increase of activity
Plus, the index is used for representing that growth of microorganism state has obvious indicative function, and carries out the chart of teledata with reference to network
Analyzing, and cross-network segment being transmitted to strange land, and monitor in real time main frame is synchronously realized in strange land, it is adjustable and real that data update the time
Existing graphic analyses, each monitoring system can at most transmit 254 terminals, can more preferably meet large-scale experiment room and scientific research organization
Need, compare that current technology needs to carry external probe and FM signal is limited transmission and compares, can more timely control culture right
The ruuning situations such as the humiture, gas concentration, electrical conductivity of elephant, so as to reach real-time monitored cell growth state continuous detecting, with
Avoid causing repeatedly to pass on, the risk for causing cell retrieve because of the multifactor culture environment of microorganism, while improving experiment
The promptness and trackability of data；Remote interaction can be realized, the early warning of abnormality, remote inquiry, remotely control are provided
Etc. function.
Description of the drawings
Fig. 1 is remote biometric cell culture monitoring and early warning aid system structural representation of the present invention；Wherein, 1, casing；2、
Gas concentration sensor；3rd, single board computer computer；4th, silver ion dividing plate；5th, water level sensor；6th, control panel；7th, measuring device wind
Fan；8th, Temperature Humidity Sensor；9th, balance pipe；10th, cabinet door；11st, oxygen sensor；12nd, conductivity sensor.
Specific embodiment
Specific embodiment one：In conjunction with Fig. 1, present embodiment, a kind of remote biometric cell culture of present embodiment are described
Device and aid system, it include casing 1, gas concentration sensor 2, single board computer computer remote on-off control system 3, silver from
Sub-dividing plate 4, water level sensor 5, control panel 6, measuring device fan 7, Temperature Humidity Sensor 8, balance pipe 9, cabinet door 10, oxygen
Sensor 11 and conductivity sensor 12；
Cavity is provided with the casing 1, cavity medial wall top is provided with measuring device fan 7, in measuring device fan 7
The cavity medial wall top of surrounding is provided with gas concentration sensor 2, Temperature Humidity Sensor 8 and oxygen around measuring device fan 7 and passes
Sensor 11；Described cavity medial wall bottom is provided with water level sensor 5 and conductivity sensor 12；Arrange in described cavity
There are multiple silver ion dividing plates 4；On described cavity medial wall, rear portion is provided with balance pipe 9；
Described 1 front side of casing is provided with cabinet door 10, and the cavity is closed by cabinet door 10；
Described control panel 6 is arranged at the top of 1 front side of casing, and is located at 10 top of cabinet door；
Described single board computer computer remote on-off control system 3 is arranged on the lateral wall on 1 right side of casing；
Described Temperature Humidity Sensor 8, gas concentration sensor 2, the signal of conductivity sensor 12 and water level sensor 5
Outfan is connected with a signal receiving end of single board computer computer remote on-off control system 3 respectively；The order of control panel 6
Signal output part is connected with another signal receiving end of single board computer computer remote on-off control system 3.
Specific embodiment two：In conjunction with Fig. 1, present embodiment, present embodiment and one difference of specific embodiment are described
It is：The inner surface material of described 1 internal cavity of casing is copper.Other compositions and connected mode and one phase of specific embodiment
With.
Specific embodiment three：In conjunction with Fig. 1, present embodiment, present embodiment and one difference of specific embodiment are described
It is：12 measurement range of conductivity sensor is 0.001 μ S/cm～300mS/cm；The minima of resolution is 0.001 μ S；Essence
Accuracy：Parameter>3 μ S are 0.5% ± 1；Parameter≤3 μ S is 0.5% ± 0.01 μ S.Other composition and connected mode be embodied as
Mode one is identical.
Specific embodiment four：In conjunction with Fig. 1, present embodiment, present embodiment and one difference of specific embodiment are described
It is：Single board computer computer remote on-off control system 3 is connected with terminal monitoring early warning software.Other composition and connected mode with
Specific embodiment one is identical.
Specific embodiment five：In conjunction with Fig. 1, present embodiment, present embodiment and one difference of specific embodiment are described
It is：Described single board computer computer remote on-off control system 3 is communicated with host computer.Other composition and connected mode with
Specific embodiment one is identical.
Specific embodiment six：A kind of remote biometric cell culture system of present embodiment and aid system are used for cultivating
Cell from mammal.
Specific embodiment seven：Present embodiment from unlike specific embodiment five：Described is dynamic from suckling
The cell of thing is primary cell, passage cell, suspension cell or attached cell.Other are identical with specific embodiment.
Specific embodiment eight：A kind of remote biometric cell culture system of present embodiment and aid system are used for cultivating
Virus.
Specific embodiment nine：A kind of remote biometric cell culture system of present embodiment and aid system are used for cultivating
Microorganism.
Specific embodiment ten：A kind of remote biometric cell culture system of present embodiment and aid system are used for cultivating
Plant cell.
Present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several specific embodiments
Contract sample can also realize the purpose that invents.
Beneficial effects of the present invention are verified by following examples：
Embodiment 1
A kind of remote biometric cell culture system of the present embodiment and aid system, it includes that casing 1, gas concentration is sensed
Device 2, single board computer computer remote on-off control system 3, silver ion dividing plate 4, water level sensor 5, control panel 6, measuring device wind
Fan 7, Temperature Humidity Sensor 8, balance pipe 9, cabinet door 10, oxygen sensor 11 and conductivity sensor 12；
The cavity that side is not closed is provided with the casing 1, and the side that cavity is not closed is by being arranged on 1 front side of casing
On cabinet door 10 closed；The cavity top medial wall is provided with measuring device fan 7, around measuring device fan 7
Cavity top medial wall is distributed with gas concentration sensor 2, Temperature Humidity Sensor 8 and oxygen sensor around measuring device fan 7
Device 11；Described cavity bottom medial wall is provided with water level sensor 5 and conductivity sensor 12；It is provided with described cavity
Multiple silver ion dividing plates 4；Balance pipe 9 is provided with described cavity rear medial wall；
Described control panel 6 is arranged at the top of 1 front side of casing, and is located at 10 top of cabinet door；
Described single board computer computer remote on-off control system 3 is arranged on the lateral wall on 1 right side of casing；
Temperature Humidity Sensor 8 is connected with control panel 6 by data wire respectively with gas concentration sensor 2；
Temperature Humidity Sensor 8, gas concentration sensor 2, conductivity sensor 12, water level sensor 5,6 points of control panel
Not Tong Guo data wire be connected with single board computer computer remote on-off control system 3.
Described cabinet door 10 is thermal-insulating sealing door；
Copper layer is provided with described 1 cavity of casing；
Described single board computer computer remote on-off control system 3 comprising remote switch control system, single board computer computer,
With sensor internet platform；
Described single board computer computer remote on-off control system 3 is equipped with brand-new ARM architecture processor, 10/100Mbps net
Mouth, built-in Ethernet, a com interface and a RS485 Interface Terminal simultaneously or independently can distribute, and support TCP
Server, TCP Client, UDP several working ways, baud rate supports 600bps～230.4Kbps, back-level server domain name
Address resolution；
Operating system supported web page is self-defined, disclosure satisfy that basic network browsing, data processing and computer programming
Needs；
Single board computer computer remote on-off control system 3 can be with place remote control device switching on and shutting down, remotely located timing
Control, remote emergency starts the functions such as UPS, reaches accurate convenient manipulation；
Sensor internet platform can carry out data lossless biography by TCP Server, TCP Client isotype
Defeated, by the high in the clouds platform of TCP Client public network server or TCP Server, local data is realized cross-network segment transmission, with
Just real time data is seen on other places are by the Internet whenever and wherever possible, while extract with same software showing pictorialization text
Part, can more intuitively be analyzed monitoring；
The present embodiment passes through its parameter feedback by arranging conductivity sensor 12, can the pure water microorganism of effective monitoring bottom
Growth conditions, by, in suspension culture microbial physiology, the research of biochemical characteristic, finding growth of microorganism and peroxidase
Activity is in parallel relation, is in contrary mirror image relationship with electrical conductivity.It is possible thereby to by cutting in difference to microorganism in pure water
Conductance under variability is flowed and (f=0.2MHz) pure electrical conductivity of water and electric capacity under resting state to measure respectively, finds micro- life
The electrical conductivity of thing changes with microbial morphology, can grow shape by quantifying germs collect, adherent sexual abnormality come microorganisms
State situation, by measuring the electric rotational spectrum of microorganism using electrorotation chip, and then detects the electricity in microorganism growth process
The rule of characteristic variations, as a result shows the increase with the time, and the electric capacity of the film of microorganism is gradually reduced, and determines on this basis thin
Bacterium, the conductance method of fungal metabolite are detecting growth of microorganism state.When their activity is relatively low, electrical conductivity is with activity
Increase and increase, the index be used for represent that growth of microorganism state has obvious indicative function, the setting electrical conductivity of the present invention
12 measurement range of sensor reaches：0.001 μ S/cm to 300mS/cm, resolution：0.001 μ S of minima, precision：Parameter>3μ
S is 0.5% ± 1；Parameter≤3 μ S is 0.5% ± 0.01 μ S.
The monitoring and early warning of the present embodiment gained is the results are shown in Table shown in 1, can be drawn by may be programmed V-COM2.10 by table 1
Software, arranges packet and obtains renewal time 1s～24h, and this example obtains a data instance with every 30s, in remote biometric cell
The warning data of aid system storage, on remote control terminal can according to time distance, in real time crawl and shown in detail in certain
Miscue and details parsing that one time point occurs, such as gas concentration, humiture etc., and help analyze appearance mistake
The analysis of causes, can also arrange conductivity threshold by programmable software, by arranging to different microorganisms conductivity threshold, point out
Deviate the ratio of threshold value, carry out remote analysis microbiological anomaly state and help experimenter to carry out the knot of in good time correction to mistake
By.
1 terminal monitoring early warning chart of table
| Date | Time | Operating system | Error message |
| 16.04.20 | 9:23:35 | Gas concentration mistake | Present value exceeds setting value |
| 15.10.09 | 15:02:43 | Relative humidity mistake | The water yield is low |
| 15.06.18 | 22:43:09 | Temperature mistake | Front temperature is too high |
| 15.01.21 | 16:22:19 | Relative humidity mistake | The water yield is low |
| 15.01.16 | 17:15:25 | Conductivity threshold mistake | Present value is more than the 20% of 0.35 μ S/cm of setting |
The announcement of book and teaching according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula carries out appropriate change and modification.Therefore, specific embodiment disclosed and described above the invention is not limited in, to this
Some modifications and changes of invention should also be as falling in the scope of the claims of the present invention.Although additionally, this specification
Used in some specific terms, but these terms are merely for convenience of description, do not constitute any restriction to the present invention.
Claims (10)
1. a kind of remote biometric cell culture system and aid system, it is characterised in that it includes casing (1), gas concentration sensing
Device (2), single board computer computer remote on-off control system (3), silver ion dividing plate (4), water level sensor (5), control panel
(6), measuring device fan (7), Temperature Humidity Sensor (8), balance pipe (9), cabinet door (10), oxygen sensor (11) and electrical conductivity
Sensor (12)；
Cavity is provided with casing (1), cavity medial wall top is provided with measuring device fan (7), in measuring device fan
(7) the cavity medial wall top around is provided with gas concentration sensor (2), Temperature Humidity Sensor (8) around measuring device fan (7)
With oxygen sensor (11)；Described cavity medial wall bottom is provided with water level sensor (5) and conductivity sensor (12)；Institute
Multiple silver ion dividing plate (4) are provided with the cavity that states；On described cavity medial wall, rear portion is provided with balance pipe (9)；
Cabinet door (10) is provided with front side of described casing (1), the cavity is closed by cabinet door (10)；
Described control panel (6) is arranged at the top on front side of casing (1), and is located above cabinet door (10)；
Described single board computer computer remote on-off control system (3) is arranged on the lateral wall on the right side of casing (1)；
Described Temperature Humidity Sensor (8), gas concentration sensor (2), conductivity sensor (12) and water level sensor (5)
Signal output part is connected with a signal receiving end of single board computer computer remote on-off control system (3) respectively；Control panel
(6) command signal outfan is connected with another signal receiving end of single board computer computer remote on-off control system (3).
2. a kind of remote biometric cell culture system according to claim 1 and aid system, it is characterised in that described
The inner surface material of casing (1) internal cavity is copper.
3. a kind of remote biometric cell culture system according to claim 1 and aid system, it is characterised in that electrical conductivity
Sensor (12) measurement range is 0.001 μ S/cm～300mS/cm；The minima of resolution is 0.001 μ S；Precision：Parameter>
3 μ S are 0.5% ± 1；Parameter≤3 μ S is 0.5% ± 0.01 μ S.
4. a kind of remote biometric cell culture system according to claim 1 and aid system, it is characterised in that single board computer
Computer remote on-off control system (3) is connected with terminal monitoring early warning software.
5. a kind of remote biometric cell culture system according to claim 1 and aid system, it is characterised in that described
Single board computer computer remote on-off control system (3) is communicated with host computer.
6. a kind of application of remote biometric cell culture system and aid system, it is characterised in that described remote biometric cell training
Foster device and aid system are used for cell of the culture from mammal.
7. the application of a kind of remote biometric cell culture system according to claim 6 and aid system, it is characterised in that
The described cell from mammal is primary cell, passage cell, suspension cell or attached cell.
8. a kind of application of remote biometric cell culture system and aid system, it is characterised in that described remote biometric cell training
Foster device and aid system are used for cultivating virus.
9. a kind of application of remote biometric cell culture system and aid system, it is characterised in that described remote biometric cell training
Foster device and aid system are used for cultivating microorganism.
10. a kind of application of remote biometric cell culture system and aid system, it is characterised in that described remote biometric cell
Culture apparatuses and aid system are used for cultivating plant cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610865859.8A CN106434343B (en) | 2016-09-29 | 2016-09-29 | A kind of remote biometric cell culture apparatus and auxiliary system and its application |
Applications Claiming Priority (1)
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