CN106417381A - Paenibacillus kribbensis oil suspension and preparation method thereof - Google Patents
Paenibacillus kribbensis oil suspension and preparation method thereof Download PDFInfo
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- CN106417381A CN106417381A CN201610843185.1A CN201610843185A CN106417381A CN 106417381 A CN106417381 A CN 106417381A CN 201610843185 A CN201610843185 A CN 201610843185A CN 106417381 A CN106417381 A CN 106417381A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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Abstract
The invention provides paenibacillus kribbensis oil suspension. The paenibacillus kribbensis oil suspension is prepared from paenibacillus kribbensis, a wetting agent, a dispersing agent, other auxiliaries and mineral oil, wherein organic bentonite or magnesium aluminum silicate is adopted as the wetting agent, fatty alcohol-polyoxyethylene ether is adopted as the dispersing agent, the other auxiliaries are phosphate polyoxyethylene nonyl phenol or WJ-630, and WG-306 or methyl oleate is adopted as the mineral oil. The invention further provides a preparation method of the paenibacillus kribbensis oil suspension. The preparation method comprises the steps that paenibacillus kribbensis, the wetting agent, the dispersing agent, the other auxiliaries and the mineral oil are put, sheared and then subjected to sand grinding, and the finished product is obtained. The paenibacillus kribbensis oil suspension is well dispersed in water in a cloudy mode; the property is stable, the layering and precipitation problems cannot be generated in the storage process, and the storage time is long; the paenibacillus kribbensis oil suspension is low in heat storage decomposition rate, cost and toxicity, stable in pesticidal efficacy, safe for human, livestock and crops and high in safety. Experiment results show that the highest disease damage prevention and control effect of the paenibacillus kribbensis oil suspension can reach 87.5%, and the paenibacillus kribbensis oil suspension can replace existing chemical pesticides.
Description
Technical field
The present invention relates to pesticide field, and in particular to a kind of Ke Liben series bacillus oil-suspending agent and preparation method thereof.
Background technology
Plant disease is divided into infectivity and two big class of non-infective.The sorting technique of the infectious disease for being caused by pathogen
Have：1. it is divided into fungoids, bacillary, viral and nematodiasis etc. by pathogen.2. by host plant be divided into crop, vegetable,
Fruit diseases and forest disease etc., can also be by stramonium leaf table
Plant disease is divided into infectivity and two big class of non-infective.The sorting technique of the infectious disease for being caused by pathogen
It is divided into fungoids, bacillary, viral and nematodiasis etc..The plant disease that plant pathogenic fungi causes is the big disease of plant three
First of, account for the 70% of all plant diseases.The disease that some crops mainly occur such as rice blast, rice sheath blight disease；Little
Wheat rust, wheat powdery mildew；Corn northern leaf blight and southern leaf blight, banded sclerotial blight；Vegetable downy mildew etc., and crops harvesting after and storage period
The disease of generation mostly is fungal disease.And for a long time, the pesticide used by controlling plant diseases is chemical bactericide mostly,
Although and had biofungicide, be difficult to prepare due to biofungicide medicament, medicament is not sufficiently stable, be difficult storage,
Particularly bactericidal effect differs larger than chemical bactericide, always cannot large-scale promotion application in actual agricultural production.
Content of the invention
In view of this, the invention provides a kind of Ke Liben series bacillus oil-suspending agent and preparation method thereof, the present invention
Ke Liben series bacillus oil-suspending agent good stability, Ke Liben series bacillus oil-suspending agent miospore germination rate height,
Enter moisture dissipate excellent, in cloud disperse, stable in properties, in storage process be not in layering and sedimentation problem, heat storage resolution ratio
Low, low cost, medicinal liquid is readily permeable to target, drug effect height；I.e. present invention firstly provides a kind of Ke Liben series bacillus are oily
Suspending agent, including following components：Ke Liben series bacillus, wetting agent, dispersant, other auxiliary agents and mineral oil.
Preferably, Ke Liben series bacillus of the present invention are bacterium of the biological deposit number for CGMCC No.7996
Kind.
Preferably, the content that Ke Liben series bacillus of the present invention account for Ke Liben series bacillus oil-suspending agent is
4%～10% weight；The Ke Liben series bacillus concentration is 1.0 × 106～1.0 × 108cfu/g.
Preferably, miscellaneous bacteria rate≤0.1% of Ke Liben series bacillus of the present invention；It is highly preferred that institute of the present invention
Suspensibility >=95% of the Ke Liben series bacillus that states；It is highly preferred that the poison of Ke Liben series bacillus of the present invention
Power≤9 (LT50, d；20-25℃).
Preferably, the content of wetting agent of the present invention is 2.5%～3.5% weight；Preferably, of the present invention
The content of dispersant is 8.0%～10.0% weight；It is highly preferred that the content of other auxiliary agents of the present invention be 4.0～
6.0% weight, balance of mineral oil.
Preferably, wetting agent of the present invention is organobentonite or Magnesiumaluminumsilicate；The dispersant is poly alkyl alcohol
Oxygen ethylene ethers；Other auxiliary agents described are thermally coupled distillation columns (NP-10P) or WJ-630 (ten thousand gold medal auxiliary agent of Shanghai
Company limited produces)；The mineral oil is WG-306 or methyl oleate.
Another object of the present invention is to provide the preparation method of above-mentioned Ke Liben series bacillus oil-suspending agent, including with
Lower step：
1) after Ke Liben series bacillus, wetting agent, dispersant, other auxiliary agents and mineral oil feeding intake, clipped, obtain
Shearing material；
2) the shearing material for obtaining the step a) carries out sand milling, obtains sand milling material；
3) the step b) is obtained the stirring of sand milling material, obtains Ke Liben series bacillus oil-suspending agent finished product.
Preferably, in preparation method of the present invention, the step 1) feeding sequence be
Bacillus cereuss, wetting agent, dispersant, other auxiliary agents are fed intake successively；The method of the shearing is to put into high shear kettle in mineral oil
Afterwards, high shear is opened, and continues shearing 20～40 minutes after feeding intake.
Preferably, in preparation method of the present invention, the step 2) the sand milling time be.
It is highly preferred that in preparation method of the present invention, the step 2) obtaining the Ke Liben series bacillus
Before oil-suspending agent finished product, also include the process for being sampled detecting.
According to technical solution of the present invention and subsequent embodiment content, the Ke Liben series bacillus oil of the present invention is outstanding
Floating agent at least has the advantage that：
1) oil-suspending agent of the present invention enter moisture dissipate excellent, in cloud disperse；
2) oil-suspending agent of the present invention is stable in properties, is not in layering and sedimentation problem, storage time in storage process
Long；Heat storage resolution ratio is low, low cost；
3) Ke Liben series bacillus oil-suspending agent low toxicity, insecticidal effect is stable, and to people and animals and crop safety, safety
High；
4) medicinal liquid of the present invention is readily permeable to target, drug effect height；Test result indicate that, Ke Liben series bacillus oil is outstanding
The effect of floating agent controlling disease reaches as high as 87.5%.
Biological deposits explanation
Ke Liben series bacillus are Ke Liben series bacillus (Paenibacillus kribbensis)；Strain name
TRCC82001, preserving number is：CGMCC No.7996, is preserved in Chinese microorganism strain preservation management on the 13rd in August in 2013
Committee's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute.
Description of the drawings
Ke Liben series bacillus oil-suspending agent preparation flow schematic diagram in Fig. 1 embodiment of the present invention.
Specific embodiment
Further technical scheme is illustrated below by way of specific embodiment, it should be understood that be only this below
Bright exemplary illustration, is not limited to the protection domain of the claims in the present invention.
Ke Liben series bacillus oil-suspending agent is provided in the embodiment of the present invention, including following components：Ke Liben class bud
Spore bacillus, wetting agent, dispersant, other auxiliary agents and mineral oil.
In one embodiment of the invention, described Ke Liben series bacillus are CGMCC for biological deposit number
The strain of No.7996.In one embodiment of the invention, described Ke Liben series bacillus cultural method is to be examined with solid
After the activation of family name's medium ii, picking single bacterium colony or lawn are inoculated into loading amount in shaking flask liquid Czapek's medium II.30 degrees Celsius,
180/min shaken cultivation 84 hours, the bacterium solution for being obtained i.e. Ke Liben series bacillus.
In another embodiment of the present invention, it is outstanding that described Ke Liben series bacillus account for Ke Liben series bacillus oil
The content of floating agent is 4%～10% weight；In yet another embodiment of the present invention, described Ke Liben series bacillus are accounted for gram
In this series bacillus oil-suspending agent content be 9% weight.
In another embodiment of the present invention, the Ke Liben series bacillus concentration is 1000 ± 50IU/mg；At this
In another embodiment of invention, the Ke Liben series bacillus concentration is 1000IU/mg
In another embodiment of the present invention, miscellaneous bacteria rate≤0.1% of described Ke Liben series bacillus；More preferably
Ground, suspensibility >=95% of Ke Liben series bacillus of the present invention；It is highly preferred that Ke Liben class bud of the present invention
Virulence≤9 (the LT of spore bacillus50, d；20-25℃).
In yet another embodiment of the present invention, the content of described wetting agent is 2.5%～3.5% weight；Preferably,
The content of dispersant of the present invention is 8.0%～10.0% weight；It is highly preferred that the containing of other auxiliary agents of the present invention
Measure as 4.0～6.0% weight, balance of mineral oil.
In yet another embodiment of the present invention, described wetting agent is organobentonite or Magnesiumaluminumsilicate；The dispersion
Agent is fatty alcohol polyethenoxy ether class；Other auxiliary agents described are thermally coupled distillation columns (NP-10P) or WJ-630
(Shanghai Wanjin Adjuvant Co., Ltd.'s product)；The mineral oil is WG-306 or methyl oleate.
In one embodiment of the invention, the preparation method of Ke Liben series bacillus oil-suspending agent is additionally provided, bag
Include following steps：
1) after Ke Liben series bacillus, wetting agent, dispersant, other auxiliary agents and mineral oil feeding intake, clipped, obtain
Shearing material；
2) the shearing material for obtaining the step a) carries out sand milling, obtains sand milling material；
3) the step b) is obtained the stirring of sand milling material, obtains Ke Liben series bacillus oil-suspending agent finished product.
In one embodiment of the invention, described step 1) described feeding sequence be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil, Ke Liben class spore bar be mineral oil,
Bacterium, wetting agent, dispersant, other auxiliary agents are fed intake successively；The method of the shearing is after mineral oil puts into high shear kettle, opens
High shear, and continue shearing 20～40 minutes after feeding intake.
In yet another embodiment of the present invention, the step 2) the sand milling time be 10～30 minutes.
In yet another embodiment of the present invention, the step 2) obtaining the Ke Liben series bacillus oil-suspending agent
Before finished product, also include the process for being sampled detecting.
If the no specified otherwise of the experimental technique used in following embodiments, is conventional method.Material used, reagent
Deng if no specified otherwise, all commercially obtaining.Cutting kettle model used 2000 in the embodiment of the present invention (resistance to speed (on
Sea) mechanical device company limited product)；Sand mill (WM50A, Qidong City Sheng Long petrochemical equipment company limited).
The preparation of this series bacillus in 1 gram of embodiment
By picking list bacterium after the solid Czapek's medium II activation of Ke Liben series bacillus CGMCCNo.7996 improvement
Fall or lawn is inoculated in liquid Czapek's medium II of the loading amount for the improvement of shaking flask 20%-30% volume.30 degrees Celsius,
180r/min shaken cultivation 84 hours, the bacterium solution for being obtained is centrifuged, and obtains supernatant and solid precipitation.Obtained
Solid be the Ke Liben series bacillus.The Czapek's medium II formula of the improvement is as follows：
Plus sterilized water is quantitative to 100%, if as solid medium then adds the agar of 1.5-2%, pH7-8.
The preparation example 1 of this series bacillus oil-suspending agent in 2 grams of embodiment
Mineral oil (methyl oleate) will be measured first put in high shear kettle, open high shear. and then successively will metering
Ke Liben series bacillus well, organobentonite, AEO-05, NP-10P put into (the sour first of surplus oiling in high shear reactor
Ester is supplied), after all materials are finished, after continuing high shear 30 minutes, carry out sand milling again.Complete for sand milling material is put into head tank
In, sample analysis after stirring, qualified rear packaging to be analyzed.
Wherein, the Ke Liben series bacillus oil-suspending agent feeds intake single for following table
| Name of material | Ingredient proportion % |
| 1*106～100*106This series bacillus in cfu/g gram | 10% |
| Organobentonite | 2.5% |
| AEO-5(AEO-3) | 10.0% |
| NP-10P(WJ-630) | 6.0% |
| Methyl oleate | Clout is supplied |
The preparation example 2 of this series bacillus oil-suspending agent in 3 grams of embodiment
Mineral oil (methyl oleate) will be measured first put in high shear kettle, open high shear. and then successively will metering
Ke Liben series bacillus well, organobentonite, AEO-05, NP-10P put into (the sour first of surplus oiling in high shear reactor
Ester is supplied), after all materials are finished, after continuing high shear 30 minutes, carry out sand milling again.Complete for sand milling material is put into head tank
In, sample analysis after stirring, qualified rear packaging to be analyzed.
Wherein, the Ke Liben series bacillus oil-suspending agent feeds intake single for following table
The preparation example 3 of this series bacillus oil-suspending agent in 4 grams of embodiment
Mineral oil (methyl oleate) will be measured first put in high shear kettle, open high shear. and then successively will metering
Ke Liben series bacillus well, organobentonite, AEO-05, NP-10P put into (the sour first of surplus oiling in high shear reactor
Ester is supplied), after all materials are finished, after continuing high shear 30 minutes, carry out sand milling again.Complete for sand milling material is put into head tank
In, sample analysis after stirring, qualified rear packaging to be analyzed.
Wherein, the Ke Liben series bacillus oil-suspending agent feeds intake single for following table
The mass parameter of Ke Liben series bacillus oil-suspending agent prepared by 5 embodiment of embodiment 2
Ke Liben series bacillus oil-suspending agent prepared by embodiment 2 carries out sample detection, as a result as follows：
In 1 gram of table, this series bacillus oil-suspending agent carries out sample detection result
In addition, found by dilution experiment etc., the oil-suspending agent of the present embodiment enters moisture and dissipates excellent, disperses in cloud；Oil
Suspending agent is stable in properties, is not in that layering and sedimentation problem, storage time length (are preserved 2 years at (4 ± 2) DEG C in storage process
Spore germination rate >=80% afterwards)；Heat storage resolution ratio low (at (25 ± 2) DEG C storage 1 year after spore germination rate (%) >=75%), becomes
This is low；3) Ke Liben series bacillus oil-suspending agent low toxicity, insecticidal effect is stable, and to people and animals and crop safety, safe
(virulence LT50≤9).
The effect experiment of Ke Liben series bacillus oil-suspending agent prepared by 6 embodiment of embodiment 2
This test is carried out in Jiangxi Province, prevents and treats kind for Oryza sativa L. Tianyou998, and management is good, and rice sheath blight disease is medium partially
Weight.Experimental field is divided into four regions by this test, and region 1 is matched group, only along ridge spray clear water；Region 2 is sprayed along ridge
The 1000 times of diluents of Ke Liben series bacillus oil-suspending agent for preparing in embodiment 2；Region 3 sprays into Ke Liben class bud along ridge
1000 times of diluents of spore bacillus wettable powder, the concentration of medicine activity component is identical with region 2；Region 4 is sprayed into gram along ridge
In 1000 times of diluents of this series bacillus aqueous suspension agent, the concentration of medicine activity component is identical with region 2 and 3；Four regions
Dosage identical, sprayed once per 7 days, spray altogether 4 times, after dispenser investigate rice sheath blight disease controlling situation.Concrete outcome is such as
Shown in table 2, can be drawn by the data of following table, the rice sheath blight disease controlling effect highest in region 2, its drug effect is above same concentration
Wettable powder and aqueous suspension agent.
The prevention effect of Ke Liben series bacillus oil-suspending agent
As seen from the above embodiment, unexpectedly, the Ke Liben series bacillus oil-suspending agent that prepared by the present invention is stable
Property high, viable count height, prevention effect is up to 87.5%, and its drug effect is obviously higher than wettable powder and the aqueous suspension of same concentration
Agent, can reach chemical medicine treatment effect.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Ke Liben series bacillus oil-suspending agent, including following components：Ke Liben series bacillus, wetting agent, dispersion
Agent, other auxiliary agents and mineral oil.
2. Ke Liben series bacillus oil-suspending agent according to claim 1, it is characterised in that the Ke Liben class spore
Bacillus is strain of the biological deposit number for CGMCC No.7996.
3. Ke Liben series bacillus oil-suspending agent according to claim 1, it is characterised in that the Ke Liben class spore
It is 4%～10% weight that bacillus accounts for the content of Ke Liben series bacillus oil-suspending agent；The Ke Liben series bacillus concentration
For 1.0 × 106～1.0 × 108cfu/g.
4. Ke Liben series bacillus oil-suspending agent according to claim 1, it is characterised in that the Ke Liben class spore
Miscellaneous bacteria rate≤0.1% of bacillus；Suspensibility >=95% of the Ke Liben series bacillus；The Ke Liben series bacillus
Virulence≤9.
5. the Ke Liben series bacillus oil-suspending agent according to claim 1-4 any one, it is characterised in that the profit
The content of humectant is 2.5%～3.5% weight, and the content of the dispersant is 8.0%～10.0% weight, other auxiliary agents described
Content be 4.0～6.0% weight, balance of mineral oil.
6. Ke Liben series bacillus oil-suspending agent according to claim 5, it is characterised in that the wetting agent is organic
Bentonite or Magnesiumaluminumsilicate；The dispersant is fatty alcohol polyethenoxy ether class；Other auxiliary agents described are Nonyl pheno
Ether phosphate (NP-10P) or WJ-630 (Shanghai Wanjin Adjuvant Co., Ltd.'s product)；The mineral oil is WG-306 or Oleic acid first
Ester.
7. a kind of preparation method of the Ke Liben series bacillus oil-suspending agent according to claim 1～6 any one, wraps
Include following steps：
1) after Ke Liben series bacillus, wetting agent, dispersant, other auxiliary agents and mineral oil feeding intake, clipped, sheared
Material；
2) the shearing material for obtaining the step a) carries out sand milling, obtains sand milling material；
3) the step b) is obtained the stirring of sand milling material, obtains Ke Liben series bacillus oil-suspending agent finished product.
8. preparation method according to claim 7, it is characterised in that the step 1) feeding sequence is mineral oil,
Ke Liben series bacillus, wetting agent, dispersant, other auxiliary agents are fed intake successively；The method of the shearing is to put in mineral oil
After high shear kettle, high shear is opened, and continues shearing 20 minutes～40 minutes after feeding intake.
9. preparation method according to claim 7, it is characterised in that the step 2) the sand milling time is 10 minutes～
30 minutes.
10. preparation method according to claim 7, it is characterised in that the step 2) obtaining the Ke Liben class bud
Before spore bacillus oil-suspending agent finished product, also include the process for being sampled detecting.
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| CN1433692A (en) * | 2003-03-05 | 2003-08-06 | 湖北省林业科学研究院 | Bacillus thuringiensis oil suspension concentrate pesticide |
| CN101611707A (en) * | 2009-08-12 | 2009-12-30 | 海南利蒙特生物农药有限公司 | A kind of bacillus subtilis oil suspending agent and preparation method thereof |
| CN103756931A (en) * | 2013-12-05 | 2014-04-30 | 江西天人生态股份有限公司 | Paenibacillus kribbensis and its application |
| CN105483053A (en) * | 2016-01-04 | 2016-04-13 | 江西天人生物控股有限公司 | Microbial compound bacteria for preventing and treating citrusyellowshoot and preparation method and application thereof |
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Application publication date: 20170222 |