CN106417001B - A kind of selection of cold tolerance at booting and flowering stage indica rice variety - Google Patents

A kind of selection of cold tolerance at booting and flowering stage indica rice variety Download PDF

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CN106417001B
CN106417001B CN201610858808.2A CN201610858808A CN106417001B CN 106417001 B CN106417001 B CN 106417001B CN 201610858808 A CN201610858808 A CN 201610858808A CN 106417001 B CN106417001 B CN 106417001B
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rice
plant
strain
booting
variety
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CN106417001A (en
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徐福荣
董超
古今
张斐斐
董鲜
汤翠凤
张恩来
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Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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Biotechnology and Germplasm Resource Institute of Yunnan Academy of Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A kind of selection of cold tolerance at booting and flowering stage indica rice variety.This method is parent, 1800 meters of Altitude Regions plantations, hybridization with moon paddy and 93-11.Its F1For plant, it is returned by male parent of 93-11, obtains B1C1F1For seed；B1C1F1Generation selfing, single plant sowing obtain B1C1F2For seed；B1C1F2To B1C1F4For plant plantation, selfing, high setting percentage strain single plant sowing is selected；B1C1F5To B1C1F6For in strain, strain plantation, systematic breeding, selection kind skin is red, setting percentage is high and compact strain single plant sowing；B1C1F7In generation, by variety test of lines, it is cold tolerance at booting and flowering stage indica rice variety to select the new lines that setting percentage is high, yield is high, cold resistance is strong, kind skin is red, plant type is compact.The kind of this method selection and breeding can be planted in height above sea level 2000m rice regions, 80% or more setting percentage, and yield is high, best in quality.

Description

A kind of selection of cold tolerance at booting and flowering stage indica rice variety
Technical field
The present invention relates to rice breeding technology field, especially a kind of choosing of cold tolerance at booting and flowering stage indica rice variety Educate method.
Background technology
Chilling injury be influence subfrigid zone area and Perenniporia martius high altitude localities Rice Production main harm it One.There are 15,000,000 hm in the whole world2Above rice makees area and is threatened by low temperature.Rice may in entire growth and development process By the harmful effect of low temperature, wherein the loss that the sterile phenomenon caused by booting florescence low temperature brings Rice Production is most Greatly.The strong and weak stabilization to high latitude, high altitude localities Rice Production of rice cold tolerance at booting and flowering stage generates important with development It influences.It is threatened by low temperature currently, the whole world has the rice of 15,000,000 ha or more to make area, China is every year because chilling injury loses rice The kg of paddy 30~5,000,000,000.In the entire growing process of rice, booting florescence is most sensitive to chilling injury, which encounters low temperature Influence to yield is also the most serious.Setting percentage is the leading indicator for evaluating booting florescence chilling damage during rice produces. In the growth and development dynamics of rice, boot stage and florescence are periods most sensitive to low temperature stress, which meets low temperature cold Evil easily causes pollen development and is pollinated bad, so as to cause the reduction of setting percentage, seriously affects the yield of rice.The two when It is closely coupled before and after phase, rice reproductive stage is belonged to, moreover under Yunnan Province's natural low temperature when progress Identification of Cold Tolerance, often Year low temperature date of occurrence is different, is not easy to judge that setting percentage reduction is caused by boot stage low temperature or florescence low temperature It is caused, therefore the cold resistance identified is known as cold tolerance at booting and flowering stage.
Rice variety have suitable for local common people's mouthfeel with that food flavor demand and exterior quality are good, commodity value is high etc. is excellent Point.But the cold resistance of rice variety is generally markedly less than japonica rice variety, and Indica Rice cannot be normal in 1600 meters of height above sea level or more It blossoms and bears fruit.Continue 3 days or more to be less than 20 DEG C in booting florescence daily mean temperature, Indica Rice is aggrieved.Meanwhile long-grained nonglutinous rice is cold-resistant Property germplasm is poor with respect to japonica rice.Therefore, carry out the breeding of Indica Rice cold resistance for meeting 1800 meters or more high altitude localities The food flavor demand of local resident is of great significance.
By the screenings of cold-resistant Rice Resources and apply in breeding work, it can be the indica rice variety applied in production Cold resistance constantly improved and improved.Meanwhile rice cold tolerance selection effect is breeder in practical breeding research In the problem of paying special attention to.Therefore, the selection on cold resistance selected test ground is extremely important.Yuanyang positioned at Yunnan Province Yuanyang County is breathed out The unique vertical agroclimate of Buddhist nun's terraced fields, the main body weather caused by the topography of height above sea level height difference great disparity and numerous national formation Powerful Nature and Man work selection pressure, results in the Local Rice Germplasm of Yuanyang Hani Terrace complexity and abundant genetic diversity.Sea The main drive that height change is rice varieties Characteristics of Vertical Distribution is pulled out, Yuanyang Hani Terrace is the differentiation of Indica round-grained rice and identification The ideal base of evaluation.
The present invention relates to the average setting percentages of indica type red rice moon paddy rice varieties be 78.56%, luffing is 62.0%~92.4%；And the setting percentage luffing of 8 parts of check varieties is larger, average out to 44.09%, wherein 4 parts of long-grained nonglutinous rice controls The setting percentage luffing of (IR36,93-11, Diantam502, Dali morning Xian) is 3.24%~58.50%, average out to 34.97%；4 parts The setting percentage luffing that japonica rice compares (Chu's round-grained rice 27, Nipponbare, 02428, collaboration 41) is 11.54%~75.63%, average out to 49.64%.Under the ecological environmental condition of 1800 meters of Yuanyang Hani Terrace locality height above sea level or more, the setting percentage of moon paddy is higher than working as All kinds of ground plantation.
Invention content
To solve Indica Rice long-grained nonglutinous rice cold resistance germplasm normally cannot be blossomed and beared fruit and widen in 1800 meters of height above sea level or more Resource, the present invention provide a kind of selection of cold tolerance at booting and flowering stage indica rice variety.
Technical scheme is as follows：
1. a kind of selection of cold tolerance at booting and flowering stage indica rice variety, includes the following steps：
The indica type red rice moon paddy rice varieties of (1) 1800 meter of Altitude Regions plantation are parent I, plant type is compact, high yield and Indica Rice improved variety 93-11 best in quality is parent II, and hybridization obtains F0For hybrid seed；
(2) F is planted0For hybrid seed, F is obtained1For plant, it is that male parent is returned with parent II, obtains B1C1F1For miscellaneous Hand over seed；
(3)B1C1F1It is selfed after being planted for hybrid seed, single plant sowing, obtains B1C1F2For seed；
(4)B1C1F2To B1C1F4For the plantation of equal plant, selfing, the strain of high setting percentage is selected to carry out single plant sowing；
(5)B1C1F5To B1C1F6For in strain, planted by strain, by systematic breeding, selection kind skin red and setting percentage High and plant type phenotype is that the elite plant strain of compact carries out single plant sowing；
(6) strain is planted to B1C1F7Generation, by variety test of lines, select setting percentage is high, yield is high, cold resistance is strong, Kind of skin red and the compact excellent Indica Rice new lines of plant type are cold tolerance at booting and flowering stage indica rice variety；
Above step (2) is carried out 1800 meters of Altitude Regions to step (6).
2. a kind of selection of cold tolerance at booting and flowering stage indica rice variety according to technical solution 1, special Sign is that the planting site of step (1) to step (6) is the Yuanyang Hani Terrace of 1800 meters of Yunnan Province Yuanyang County height above sea level.
Compared with prior art, innovative point of the invention and advantageous effect are：
1, donor parents cold resistance is strong：The Indica Rice germplasm moon paddy with strong cold resistance is searched out to supply as cold resistance Body parent.
2, select variable rate technology it is excellent, be Yunnan to the ideal base of Indica Rice cold tolerance at booting and flowering stage characterization and evaluation Province Yuanyang County Yuanyang Hani Terrace.
3, representative character：Kind skin is red.
4, the cold resistance indica rice variety selected using the method for the present invention can be planted in height above sea level 2000m rice regions, and be tied Real rate is up to 80% or more, and yield is high, best in quality, solves Indica Rice in 1800 meters of height above sea level or more the knot that cannot normally bloom Real technical problem, while having widened long-grained nonglutinous rice cold resistance germ plasm resource.
Specific implementation mode
Embodiment
Material to be tested：Moon paddy, moon paddy are indica rice variety, red rice.93-11,93-11 are indica rice varieties. Moon paddy and 93-11 are commercial product.Without specified otherwise it is conventional method in embodiment.
Experimental field：1800 meters of Altitude Regions of Yunnan Province Yuanyang County Yuanyang Hani Terrace.
(1) indica type red rice rice varieties moon paddy is planted in 1800 meters of Altitude Regions of Yunnan Province Yuanyang County Yuanyang Hani Terrace For parent I, it is parent II to select compact plant type, high yield and Indica Rice improved variety 93-11 best in quality, and hybridization obtains F0 For hybrid seed.
II 93-11 of parent is the middle Xian kinds cultivated by Jiangsu Province's LX-river area Institute of agricultural sciences, is had powerful Biological superiority, ideal plant type, stronger resistance, yield and quality characters are excellent, are commonly used for rice breeding, are mesh The male parent of preceding hybrid paddy rice main breed two line system etc., II 93-11 of parent, setting percentage 88.0%, mass of 1000 kernel 31.0g, simple grain production 27.57g is measured, grain length 6.9mm dislikes white rate 32%.Described best in quality good for exterior quality, food flavor is good.
(2) by F0It is planted in the rice terrace at 1800 meters of Yunnan Province Yuanyang County Yuanyang Hani Terrace for hybrid seed, obtains F1 For plant, it is that male parent is returned with parent II, obtains B1C1F1For hybrid seed；
(3)B1C1F1It is selfed after being planted for hybrid seed, single plant sowing, obtains B1C1F2For seed；
(4)B1C1F2To B1C1F4For the plantation of equal plant, selfing, the strain of high setting percentage is selected to carry out single plant sowing；
(5)B1C1F5And B1C1F6For in strain, planted by strain, by systematic breeding, selection kind skin red and setting percentage 80% or more, and the elite plant strain that plant type is compact carries out single plant sowing；The elite plant strain be red rice, setting percentage 80% with Upper and plant type is compact；
(6) strain is planted to B1C1F7Generation, by variety test of lines, select setting percentage is high, yield is high, cold resistance is strong, Kind of skin red and the compact excellent Indica Rice new lines of plant type are cold tolerance at booting and flowering stage indica rice variety；It is described excellent Good Indica Rice new lines refer to red rice, 80% or more setting percentage, plant type is compact and yield is significantly higher than moon paddy；It is described cold-resistant Property refer to by force that common variety cannot be normal solid under conditions of low temperature stress, and Seed-setting Rate of Varieties normally i.e. 80% or more. The average setting percentage of the cold tolerance at booting and flowering stage indica rice variety selected is 82.3%, average yield per mu 450kg, this product Kind is best in quality, and brown rice is 12.7mg/kg rich in trace elements iron, can be planted in height above sea level 2000m rice regions.
Above step (3) is planted in 1800 meters of Altitude Regions of Yunnan Province Yuanyang County Yuanyang Hani Terrace to step (6).

Claims (1)

1. a kind of selection of cold tolerance at booting and flowering stage indica rice variety, it is characterised in that include the following steps：
The indica type red rice moon paddy rice varieties of (1) 1800 meter of Altitude Regions plantation are parent I, and plant type is compact, high yield and quality Excellent Indica Rice improved variety 93-11 is parent II, and hybridization obtains F0 for hybrid seed；
(2) plantation F0 obtains F1 generation plant for hybrid seed, is that male parent is returned with parent II, obtains B1C1F1 generation hybridization Seed；
(3) B1C1F1 is selfed after being planted for hybrid seed, single plant sowing, obtains B1C1F2 for seed；
(4) B1C1F2 to B1C1F4 is planted for equal plant, is selfed, and the strain of high setting percentage is selected to carry out single plant sowing；
(5) B1C1F5 to B1C1F6 is planted by strain in strain, by systematic breeding, selection kind skin is red, setting percentage is high and Plant type phenotype is that the elite plant strain of compact carries out single plant sowing；
(6) strain was planted to B1C1F7 generations, by variety test of lines, selected that setting percentage is high, yield is high, cold resistance is strong, kind The excellent Indica Rice new lines that skin is red, plant type is compact are cold tolerance at booting and flowering stage indica rice variety；
The planting site of step (1) to step (6) is the Yuanyang Hani Terrace of 1800 meters of Yunnan Province Yuanyang County height above sea level.
CN201610858808.2A 2016-09-28 2016-09-28 A kind of selection of cold tolerance at booting and flowering stage indica rice variety Active CN106417001B (en)

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CN1411701A (en) * 2002-11-21 2003-04-23 江西省农业科学院水稻研究所 Screening method for breeding strong cold-resisting new rice variety
CN103461111A (en) * 2013-09-27 2013-12-25 江苏丘陵地区镇江农业科学研究所 Breeding method for high-cold-resistance rice

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CN103392591B (en) * 2013-07-05 2016-08-10 安徽省农业科学院水稻研究所 A kind of breeding of new variety method that rice high yield and nitrogen efficiently utilize
CN104686317A (en) * 2015-02-04 2015-06-10 湖南省蔬菜研究所 Method for breeding multi-bolting cold-resistant flowering cabbage
CN105660366A (en) * 2016-02-01 2016-06-15 浙江纳寿农业科技发展有限公司 Breeding method of nutritious rice variety

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Publication number Priority date Publication date Assignee Title
CN1411701A (en) * 2002-11-21 2003-04-23 江西省农业科学院水稻研究所 Screening method for breeding strong cold-resisting new rice variety
CN103461111A (en) * 2013-09-27 2013-12-25 江苏丘陵地区镇江农业科学研究所 Breeding method for high-cold-resistance rice

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