CN1064081C - 造血干细胞、祖细胞以及巨核细胞的扩增方法 - Google Patents
造血干细胞、祖细胞以及巨核细胞的扩增方法 Download PDFInfo
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- CN1064081C CN1064081C CN94113972A CN94113972A CN1064081C CN 1064081 C CN1064081 C CN 1064081C CN 94113972 A CN94113972 A CN 94113972A CN 94113972 A CN94113972 A CN 94113972A CN 1064081 C CN1064081 C CN 1064081C
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Description
细胞经48小时液体培养 | 加PF4(5μg/毫升)孵育 | 不加PF4孵育 |
细胞数量增加 | 1.98倍 | 3.24倍 |
细胞倍增时间(小时) | 35 | 26 |
细胞各时期百分数G11S1G2/M2 | 48.845.55.7 | 59.429.111.5 |
细胞各时期所需时间(小时)Gl2S1G2/M1 | 16.915.82 | 15.57.63 |
因子 浓度(/ml) | HPP-CFC | CFU-MK | CFU-GM | BFU-E |
无 | 6±1/105细胞 | 52±8/105细胞 | 49±6/105细胞 | 16±2/105细胞 |
PF4 5μg | 7±1/105细胞 | 14±3/105细胞* | 28±4/105细胞# | 9±1/105细胞# |
TGF-β1 1μg | 7±0.8/105细胞 | 12±2/105细胞* | 11±2/105细胞# | 4±1/105细胞* |
ZD 5μg | 6±0.5/105细胞 | 78±9/105细胞* | 56±5/105细胞 | 18±2/105细胞 |
PF4+ZD | 7±1/105细胞 | 54±11/105细胞 | 53±5/105细胞 | 14±1/105细胞 |
TGF-β 1+ZD | 5±1/105细胞 | 52±8/105细胞 | 46±3/105细胞 | 13±2/105细胞 |
浓度(/ml) | CFU-MK | CFU-GM | BFU-E |
ZD 00.1μg1μg#10μg100μg | 7±121±3#29±3*27±2*25±3* | 15±217±313±218±315±2 | 00000 |
MP0 1μg | 35±4 | 18±2 | 1±0.7 |
ZD+MP0 | 72±4# | 21±3 | 2±1 |
IL6 20ng | 18±2 | 20±2 | 0 |
ZD+IL6 | 38±3# | 24±3 | 2±1 |
GM-CSF 10ng | 22±4 | 45±3 | 0 |
ZD+GM-CSF | 23±3 | 78±3# | 0 |
EP0 1U | 19±3 | 17±3 | 37±3 |
ZD+EP0 | 25±3 | 19±3 | 68±4# |
藻酸双脂钠剂量(μg/次) | 巨核细胞数±103/股骨 | 巨核细胞祖细胞数×103/股骨 | 粒细胞祖细胞数×103/股骨 | 白细胞数×109/升 | 血红蛋白克/100毫升 | 血小板数×109/升 |
0 | 5.3±0.3 | 1.5±0.1 | 2.5±0.2 | 3.2±0.3 | 14.0±0.5 | 890±58 |
1 | 6.2±0.5* | 2.5±0.2 | 2.6±0.3 | 2.9±0.4 | 14.2±0.4 | 966±78 |
10 | 6.9±0.7* | 3.1±0.5* | 2.7±0.2 | 3.2±0.6 | 14.1±0.5 | 1120±82* |
50 | 6.8±0.5* | 2.9±0.4* | 2.6±0.3 | 3.3±0.5 | 14.5±0.7 | 1050±76* |
HPP-CFC | CFU-MK | CFU-GK | BFU-E | |
未与因子孵育的骨髓细胞 | 4±1/105细胞 | 25±4/105细胞 | 32±6/105细胞 | 24±3/105细胞 |
未与因子孵育的骨髓细胞+ZD洗涤 | 5±1/105细胞 | 32±3/105细胞 | 34±4/105细胞 | 28±3/105细胞 |
PF4孵育的骨髓细胞 | 6±2/105细胞 | 13±2/105细胞# | 19±3/105细胞# | 14±2/105细胞# |
PF4孵育的骨髓细胞+ZD洗涤 | 5±2/105细胞 | 27±2/105细胞 | 33±4/105细胞 | 23±3/105细胞 |
骨髓细胞 生长因子 | HPP-CFC | CFU-MK | CFU-GK |
未与因子孵育的骨髓细胞 IL3+IL6 | 7±2/105细胞 | 34±3/105细胞 | 44±7/105细胞 |
未与因子孵育的骨髓细胞 SCF | 14±3/105细胞 | 14±2/105细胞 | 18±3/105细胞 |
PF4孵育的骨髓细胞# IL3+IL6 | 38±2/105细胞* | 56±2/105细胞* | 54±4/105细胞# |
PF4孵育的骨髓细胞# SCF | 45±3/105细胞* | 49±4/105细胞* | 35±1/105细胞 |
CD 34+细胞 | CFU-GM | CFU-MK | BFU-E | |
再障血清 | 1.8% | 26/2×105细胞 | 45/2×105细胞 | 28/2×105细胞 |
再障血清+ZD | 25% | 32/2×105细胞 | 91/2×105细胞 | 35/2×105细胞 |
脐带血清 | 17% | 22/2×105细胞 | 41/2×105细胞 | 19/2×105细胞 |
脐带血清+ZD | 23% | 29/2× 105细胞 | 78/2×105细胞 | 41/2×105细胞 |
SCF+IL3+IL6+GM-CSF+EPO | 19% | 29/2×105细胞 | 40/2×105细胞 | 36/2×105细胞 |
SCF+IL3+IL6+GM-CSF+EPO+ZD | 26% | 41/2×105细胞 | 76/2×105细胞 | 56/2×105细胞 |
HPP-CFC | CFU-GM | CFU-MK | BFU-E | |
PF4+再障血清 | 2.50 | 1.52 | 2.72 | 1.34 |
PF4+再障血清+ZD | 3.61 | 1.72 | 3.80 | 1.63 |
PF4+脐带血清 | 2.43 | 1.43 | 2.62 | 1.36 |
PF4+脐带血清+ZD | 3.62 | 1.62 | 3.45 | 1.72 |
PF4+IL3+IL6 | 1.70 | 1.72 | 2.15 | 1.32 |
PF4+IL3+IL6+ZD | 3.10 | 1.93 | 3.23 | 1.56 |
PF4+SCF+GM-CSF | 2.12 | 3.12 | 1.17 | 1.22 |
PF4+SCF+GM-CSF+ZD | 3.12 | 3.52 | 1.64 | 1.42 |
PF4+SCF+EPO | 1.68 | 1.43 | 1.21 | 3.12 |
PF4+SCF+EPO+ZD | 2.72 | 1.62 | 1.72 | 3.74 |
PF4+SCF+bFGF | 2.37 | 1.72 | 1.67 | 2.10 |
PF4+SCF+bFGF+ZD | 3.64 | 2.10 | 2.24 | 2.42 |
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN94113972A CN1064081C (zh) | 1994-11-04 | 1994-11-04 | 造血干细胞、祖细胞以及巨核细胞的扩增方法 |
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CN94113972A CN1064081C (zh) | 1994-11-04 | 1994-11-04 | 造血干细胞、祖细胞以及巨核细胞的扩增方法 |
Publications (2)
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CN1122368A CN1122368A (zh) | 1996-05-15 |
CN1064081C true CN1064081C (zh) | 2001-04-04 |
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CN94113972A Expired - Lifetime CN1064081C (zh) | 1994-11-04 | 1994-11-04 | 造血干细胞、祖细胞以及巨核细胞的扩增方法 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1353994B (zh) * | 2001-11-19 | 2011-01-12 | 上海市儿童医院 | 一种建立人/山羊造血干细胞异种移植模型的方法 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1302102C (zh) * | 2003-12-29 | 2007-02-28 | 中国医学科学院血液学研究所 | 通过扩增巨核祖细胞和成熟巨核细胞制备巨核细胞制剂的方法及用途 |
CN100572528C (zh) * | 2004-09-28 | 2009-12-23 | 中国人民解放军军事医学科学院野战输血研究所 | 一种体外扩增造血干/祖细胞的方法 |
-
1994
- 1994-11-04 CN CN94113972A patent/CN1064081C/zh not_active Expired - Lifetime
Non-Patent Citations (4)
Title |
---|
B10001,V01,76 1919.9.1 HAM等 * |
B10001,V01,76 1919.9.1 HAM等;BVJ,HFAMTOIV01,81 1919.9.2 HAM等;JLAB8CUMABV01,123 1919.9.4 HAM * |
BVJ,HFAMTOIV01,81 1919.9.2 HAM等 * |
JLAB8CUMABV01,123 1919.9.4 HAM * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1353994B (zh) * | 2001-11-19 | 2011-01-12 | 上海市儿童医院 | 一种建立人/山羊造血干细胞异种移植模型的方法 |
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