CN106399455A - Greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco - Google Patents
Greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco Download PDFInfo
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- CN106399455A CN106399455A CN201610782753.1A CN201610782753A CN106399455A CN 106399455 A CN106399455 A CN 106399455A CN 201610782753 A CN201610782753 A CN 201610782753A CN 106399455 A CN106399455 A CN 106399455A
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Abstract
The invention provides a greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco. The method comprises the following steps: I, cultivating tobacco alternaria alternata germs on a PDA plate medium, and preparing inoculation fluid; II, transplanting tobacco into a flowerpot; and III, spraying the inoculation fluid to plants after the growth of tobacco true leaves and conducting inoculating, conducting cultivation and identifying the disease-resistance strength. According to the greenhouse seedling stage inoculation identification method of the disease-resistance strength of resisting to the alternaria alternata of tobacco, the scientific and reasonable identification method and process parameter control are set in accordance with researches on infection processes and infection parameters of the alternaria alternata; and meanwhile, in combination with the growth properties of the tobacco plants as well as local disease resistance of the plants, disease-resistance strength of resisting to the alternaria alternata of tobacco is effectively and reasonably identified
Description
Technical field
The present invention relates to microbial control field, particularly relate to a disease resistance power greenhouse seedling stage growing tobacco anti-rust
Inoculation identification method.
Background technology
Alternaria alternate is one of Major Diseases on China's tobacco leaf production.China respectively produces cigarette district sending out of this disease
Raw, it can infect blade, stem, bennet and capsule etc..How blade occurs from lower blade if being caught an illness, gradually develop upwards.At the beginning of scab it is
Yellowish-brown fleck, after develop into brown circle or subcircular spot, upper tool is russet or dark brown concentric wheel stripe.When extending very fast, side
Yellow halo in edge.Visible dark brown or the mould layer of black on spot during high humidity.Scab matter is crisp, fragile.When serious, scab merges
Blade is made to become broken leaf.Vein, bennet, capsule, stem catch an illness existing oblong or fusiformis dark brown depression spot.
According to the pests occurrence rule of rust, the features such as (First aggression, route of transmission, infect again), for the preventing and treating of rust,
Should adopt to plant disease-resistant variety, chemical control and to implement the comprehensive preventive health measures that reasonable cultivation step combines, and for such as
What filters out disease-resistant variety, then be crucial, in traditional screening, for the screening of rust disease-resistant variety, is not formed relatively
For unified screening technique, cause the disunity on screening technique, the difference of screening effect.
Content of the invention
The present invention proposes a disease resistance power greenhouse Seedling Inoculation authentication method growing tobacco anti-rust, method rationally,
Qualification result accurately and reliably, can be applicable on a large scale.
The technical scheme is that and be achieved in that：The one disease resistance power greenhouse Seedling Inoculation growing tobacco anti-rust
Authentication method, comprises the following steps：
Step one：Tobacco brown spot pathogen is cultivated on PDA plate culture medium, and configures inoculation liquid；
Step 2：By in tobacco transplant to flowerpot；
Step 3：After tobacco seedlings grow true leaf, described inoculation liquid is sprayed on plant and is inoculated, cultivate and identify disease-resistant
Property strong and weak.
Further, in step one, tobacco brown spot pathogen is cultivated 7 days for 26 DEG C on PDA plate culture medium, described inoculation liquid
It is that to be made into concentration with 1% Glucose Liquid be 1*105The spore suspension of CFU/ml, the setting of this parameter ensure that inoculation liquid
Activity and validity.
Further, the diameter of described flowerpot is not less than 2 plants of transplanting in 10cm, each described flowerpot, reduces and transplants cost, with
When do not result in mutual impact, and tobacco transplant 20 basin of each kind, and set repetition, no less than 3 times it is ensured that identification
The accuracy of result and reliability.
Further, in step 3, when tobacco seedlings grow 9-10 piece true leaf, formed certain plant, now connect
Kind, result is relatively reliable.
Further, in step 3, it is incubated in growth cabinet and carries out, 26-30 DEG C of design temperature, relative humidity 70%,
The setting of these parameters is easy to be controlled the factor of catching an illness, and prevents other germs from disturbing, is just also allowing for brown spot pathogen simultaneously
Often contaminate.
Further, for improve accuracy, also set susceptible variety in identification simultaneously, disease-resistant variety as comparison, and
The local disease-resistant variety of local plantation, in locality, anti-kind and local susceptible variety are as auxiliary comparison.
Further, in step 3, after inoculation the 7th, 10,20d investigation occurring degree, this 3 stages correspond to red respectively
3 stages of star germ development.
Further, Disease Resistance Identification is classified investigation in units of blade, and according to the identification of following hierarchical approaches：
0 grade：Full leaf is anosis；
1 grade：Lesion area accounts for less than the 1% of blade area；
3 grades：Lesion area accounts for the 2-5% of blade area；
5 grades：Lesion area accounts for the 6-10% of blade area；
7 grades：Lesion area accounts for the 11-20% of blade area；
9 grades：Lesion area accounts for more than the 21% of blade area.
The disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of described tobacco of the present invention, according to rust
Dip-dye process and the parameter study contaminated, are set scientific and reasonable authentication method and are controlled with procedure parameter, in combination with
The growth characteristics of tobacco plant itself, and have also combined the disease-resistant situation of the plant of locality, anti-to the anti-rust of tobacco
Sick power carried out effectively, rational identify, large-area promoting the use of can be carried out.
Specific embodiment
Below in conjunction with embodiments of the invention, the technical scheme in the embodiment of the present invention is clearly and completely retouched
State it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the present invention
In embodiment, the every other enforcement that those of ordinary skill in the art are obtained under the premise of not making creative work
Example, broadly falls into the scope of protection of the invention.
Embodiment 1
The inoculated identification of (Guangdong) greenhouse seedling stage anti-rust
1. the preparation of inoculum
By tobacco brown spot pathogen in the upper 26 DEG C of culture 7d of PDA plate culture medium (potato dextrose agar), use
It is 1*10 that 1% Glucose Liquid is made into concentration5The spore suspension of CFU/ml is as inoculation liquid.
2. the preparation of inoculation material
By tobacco transplant to diameter greater than in the flowerpot of 10cm, not having kind 20 basin, 2 plants of every basin, at least to set 3 repetitions.
3. inoculation method
Carry out rust spore (1*10 when tobacco seedlings 9-10 piece true leaf5CFU/ml) inoculate, will with small hand-held sprayer
Spore suspension is sprayed onto on plant, is subsequently placed in growth cabinet, 26-30 DEG C of design temperature, relative humidity 70%, if sense
Sick kind, disease-resistant variety is as comparison, and the local disease-resistant variety of local plantation, anti-kind and local susceptible variety in locality
As auxiliary comparison.
4. investigation and identification
Control time：After inoculation the 7th, 10,20d investigation occurring degree.
Identification.
Embodiment 2-3
With embodiment 1, difference, embodiment 2-3 method is that embodiment 2 is carried out in Shandong, embodiment 3 is carried out in Yunnan.
Qualification result shows, Guangdong, Yunnan, and the disease resistance in Shandong is more or less the same, and (in greenhouse, disease severity differs not
Greatly), data is as follows：
| 7d (%) | 10d (%) | 20d (%) | Disease severity (level) | |
| Embodiment 1 | 1 | 2 | 4 | 3 |
| Embodiment 2 | 1 | 3 | 8 | 5 |
| Embodiment 3 | 1 | 2 | 5 | 3 |
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement made etc., should be included in the protection of the present invention.
Claims (9)
1. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco is it is characterised in that comprise the following steps：
Step one：Tobacco brown spot pathogen is cultivated on PDA plate culture medium, and configures inoculation liquid；
Step 2：By in tobacco transplant to flowerpot；
Step 3：After tobacco seedlings grow true leaf, described inoculation liquid is sprayed on plant and is inoculated, cultivate and identify that disease resistance is strong
Weak.
2. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as described in the appended claim 1, its feature exists
In：In step one, tobacco brown spot pathogen is cultivated 7 days for 26 DEG C on PDA plate culture medium, and described inoculation liquid is to use 1% glucose
It is 1*10 that liquid is made into concentration5The spore suspension of CFU/ml.
3. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as described in the appended claim 1, its feature exists
In：The diameter of described flowerpot is not less than in 10cm, each described flowerpot transplants 2 plants.
4. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as claimed in claim 3, its feature exists
In：Tobacco transplant 20 basin of each kind, and set repetition, no less than 3 times.
5. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as described in the appended claim 1, its feature exists
In：In step 3, when tobacco seedlings grow 9-10 piece true leaf, inoculated.
6. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as described in the appended claim 1, its feature exists
In：In step 3, it is incubated in growth cabinet and carries out, 26-30 DEG C of design temperature, relative humidity 70%.
7. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as described in the appended claim 1, its feature exists
In：Also set susceptible variety, disease-resistant variety is as comparison, and the local disease-resistant variety of local plantation, anti-in locality simultaneously
Kind and local susceptible variety are as auxiliary comparison.
8. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as described in the appended claim 1, its feature exists
In：In step 3, after inoculation the 7th, 10,20d investigation occurring degree.
9. the disease resistance power greenhouse Seedling Inoculation authentication method of the anti-rust of tobacco as described in the appended claim 1, its feature exists
In：Disease Resistance Identification is classified investigation in units of blade, and according to the identification of following hierarchical approaches：
0 grade：Full leaf is anosis；
1 grade：Lesion area accounts for less than the 1% of blade area；
3 grades：Lesion area accounts for the 2-5% of blade area；
5 grades：Lesion area accounts for the 6-10% of blade area；
7 grades：Lesion area accounts for the 11-20% of blade area；
9 grades：Lesion area accounts for more than the 21% of blade area.
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| CN201610782753.1A CN106399455A (en) | 2016-08-30 | 2016-08-30 | Greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco |
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| CN201610782753.1A CN106399455A (en) | 2016-08-30 | 2016-08-30 | Greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109328683A (en) * | 2018-09-30 | 2019-02-15 | 山西省农业科学院植物保护研究所 | A method of quinoa downy mildew disease resistance is identified using cutting propagation |
| CN110622720A (en) * | 2019-10-14 | 2019-12-31 | 贵州省烟草科学研究院 | Inoculation method of alternaria alternate |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103756915A (en) * | 2014-01-14 | 2014-04-30 | 云南省烟草农业科学研究院 | Alternaria alternata inoculating liquid and preparation method thereof |
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2016
- 2016-08-30 CN CN201610782753.1A patent/CN106399455A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103756915A (en) * | 2014-01-14 | 2014-04-30 | 云南省烟草农业科学研究院 | Alternaria alternata inoculating liquid and preparation method thereof |
Non-Patent Citations (2)
| Title |
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| 孙计平等: "烟草黑茎病及其抗性鉴定方法研究进展", 《河南农业科学》 * |
| 徐博等: "烟草种质资源田间抗赤星病鉴定试验", 《农业科技通讯（实验研究）》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109328683A (en) * | 2018-09-30 | 2019-02-15 | 山西省农业科学院植物保护研究所 | A method of quinoa downy mildew disease resistance is identified using cutting propagation |
| CN109328683B (en) * | 2018-09-30 | 2020-09-18 | 山西农业大学 | Method for identifying downy mildew disease resistance of quinoa by utilizing cutting propagation |
| CN110622720A (en) * | 2019-10-14 | 2019-12-31 | 贵州省烟草科学研究院 | Inoculation method of alternaria alternate |
| CN110622720B (en) * | 2019-10-14 | 2021-08-24 | 贵州省烟草科学研究院 | Inoculation method of alternaria alternate |
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Application publication date: 20170215 |