CN106397624A - Aqueous extraction method of cinnamon polysaccharides - Google Patents
Aqueous extraction method of cinnamon polysaccharides Download PDFInfo
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- CN106397624A CN106397624A CN201610955372.9A CN201610955372A CN106397624A CN 106397624 A CN106397624 A CN 106397624A CN 201610955372 A CN201610955372 A CN 201610955372A CN 106397624 A CN106397624 A CN 106397624A
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- cinnaman
- extraction
- cinnamon
- water
- polysaccharides
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
Abstract
The invention discloses an aqueous extraction method of cinnamon polysaccharides. The aqueous extraction method of the cinnamon polysaccharides comprises the following steps: cinnamomum is smashed into 60 meshes, petroleum ether is added for reflux and degreasing, the solid-liquid ratio is 1:20, and extraction with hot water is performed after a solvent is volatilized; the extraction temperature is 100 DEG C, the pH value is 5, and the extraction time lasts for 2h; an extract liquid is filtered, concentrated and precipitated with absolute ethanol with the volume being eight times that of the extract liquid, suction filtration is performed, filter residues are obtained, dried and washed with absolute ethanol three times, vacuum drying is performed, and the cinnamon polysaccharides are obtained. With the yield of water-soluble polysaccharides taken as the index, the single-factor experiment and orthogonal experiment research of the extraction process of the water-soluble cinnamon polysaccharides is performed, extraction optimized technological parameters of the cinnamon polysaccharides is obtained, and scientific basis is provided for the research and development of cinnamon. The reproducibility of optimized conditions is good, and industrial production is facilitated. The cinnamon total polysaccharides extracted with the method has an effect in reducing blood glucose of mice with diabetes, and a theoretical basis is provided for further development of medicinal value of cinnamomum.
Description
Technical field
The invention belongs to technical field of natural product extraction, more particularly, to a kind of water extracting method of cinnaman.
Background technology
Cortex Cinnamomi (Cinnamomum cassia presl), canella, its dry bark for China Ministry of Public Health announce i.e.
Food can be done and can do one of article of medicine again.China is Cortex Cinnamomi main producing region, Cortex Cinnamomi aboundresources.Contained by Cortex Cinnamomi, chemical composition has
Volatile oil, phenols, organic acid, tannin, polysaccharide, glycoside, steroidal, lactone, coumarin etc..Pharmacological testing shows, cinnaman has
Hypoglycemic physiologically active.There is a kind of extracting method of cinnaman of reported in literature：Cortex Cinnamomi is ground into coarse powder, plus 5 times amount
Petroleum ether flows back 2 times, each 2h, filters；Filtering residue natural air drying, plus 10 times amount dehydrated alcohol reflux, extract, 3 times, each 1.5h,
Filter；Filtering residue natural air drying, plus 10 times amount, 80% alcohol reflux 3 times, each 1.5h, filters；Filtering residue natural air drying, plus 10
Times amount water reflux, extract, 4 times, each 1.5h, filters.After filtrate concentrates, plus dehydrated alcohol makes alcohol content reach 30% to carry out precipitate with ethanol,
Centrifugation；Filtrate is flung to ethanol and is added dehydrated alcohol and make alcohol content reach 50% and carries out precipitate with ethanol, centrifugation；Filtrate is flung to ethanol and is added dehydrated alcohol
Make alcohol content reach 80% and carry out precipitate with ethanol, centrifugation.Precipitation is dissolved in water, and removes isolating protein with Sevage method, successively through dehydrated alcohol,
Acetone, absolute ether washing, cold drying obtains final product cinnaman extract.Also has a kind of extraction of cinnaman of reported in literature
Method：Cortex Cinnamomi pulverizer is crushed to and can pass through 40 mesh sieves, apparatus,Soxhlet'ses defat, uses hot water extraction after volatilizing solvent.Extract
Behind through filtering, being concentrated into original volume 1/4 to 1/5, plus 95% ethanol of 3 times of its volumes, it is placed in alcohol analysis 24h in refrigerator, obtain
To flocculent deposit, it is centrifuged 10min, removes supernatant；Gained precipitation is respectively washed 3 times with dehydrated alcohol, acetone, absolute ether successively,
Centrifugation 5min, removes supernatant, collects precipitation, vacuum drying, obtains final product cinnaman.Also has a kind of cinnaman of reported in literature
Extracting method：Take 20.0g Cortex Cinnamomi medicated powder, compare 1: 20 (V/V) by medicinal liquid, carried with petroleum ether, ether, each backflow of 80% ethanol successively
Take 4h, medicinal residues volatilize after solvent with 20 times of distilled water reflux, extract, 4h, and extracting solution is concentrated into half volume, decolouring, filtration, filtrate
Add dehydrated alcohol to make alcohol content reach 80%, stand overnight, filtration, residue ether, acetone, dehydrated alcohol cyclic washing number
Secondary, obtain polysaccharide, put drying for standby in silica gel drier.
In sum, the extraction process of existing cinnaman is relatively complicated, and required cost is larger, produces difficult.
Content of the invention
It is an object of the invention to provide a kind of water extracting method of cinnaman is it is intended to solve existing cinnaman
Extraction process is relatively complicated, and required cost is larger, produces difficult problem.
The present invention is achieved in that a kind of water extracting method of cinnaman, the water extracting method of described cinnaman
Solid-liquid ratio 1:20,100 DEG C of Extracting temperature, pH value 5, extraction time 2h.
Further, the water extracting method of described cinnaman comprises the following steps：
Step one, Cortex Cinnamomi is crushed to 60 mesh, plus petroleum ether backflow defat, solid-liquid ratio 1:20, soaked with hot water after volatilizing solvent
Carry；100 DEG C of Extracting temperature, pH value 5, extraction time 2h；
Step 2, after filtering, concentrating plus 8 times of volume dehydrated alcohol are precipitated extracting solution, and sucking filtration obtains filtering residue, dries,
With absolute ethanol washing 3 times, vacuum drying, obtain final product cinnaman.
Another object of the present invention is to providing the Cortex Cinnamomi that a kind of water extracting method using described cinnaman extracts many
Sugar.
Another object of the present invention is to providing a kind for the treatment of sugar of the preparation of the water extracting method using described cinnaman
Urine medicine.
The water extracting method of the cinnaman that the present invention provides, with water soluble polysaccharide yield as index, to Cortex Cinnamomi water solublity
The extraction process of polysaccharide has carried out single factor experiment and orthogonal test research, obtains the optimize technique ginseng of cinnaman extraction
Number, provides scientific basis for research and development Cortex Cinnamomi.Optimum condition repeatability is good, beneficial to industrialized production.Solid-liquid ratio 1:20, carry
Take 100 DEG C of temperature, pH value 5, extraction time 2h, scheme is simple, easily amplify and produce.
There is the effect of the blood glucose reducing diabetic mice using the Cortex Cinnamomi total polysaccharidess that the present invention extracts, for developing further
The medical value of Cortex Cinnamomi provides theoretical basiss.At present, diabetes have become one of high disease of global prevalence, wherein with
Type 2 diabetes mellitus are particularly common, and its complication is more, and hazardness is very big.Development with social economy and living-pattern preservation,
Global type 2 diabetes mellitus sickness rate constantly rises.It is about 2～4 in China's onset diabetes rate.Type 2 diabetes mellitus include two important
Pathophysiology feature：Insulin resistant and insulopathic.Wherein insulin resistant again with the generations such as obesity, hyperlipemia
Thank to disorder to link together.Clinical and experimental study in recent years proves, Cortex Cinnamomi is in treatment diabetes and abnormalities of sugar/lipid metabolism
Disease aspect has definite curative effect, really can play blood sugar lowering, the effect of regulation blood fat.In terms of anti-diabetic, forefathers, thinking sugar more
The sick pathogenesis of urine is with " deficiency of YIN for this, scorching for mark ", but is as advancing of disease, the Syndrome in TCM that most type 2 diabetes mellitus are fallen ill
Wait 3 stage development processes not necessarily through intense heat due to deficiency of YIN deficiency of both QI and YIN deficiency of both YIN and YANG, yang deficiency syndrome is in disease
Each period of journey all may occur in which, appropriate gives warming YANG medicine, such as Cortex Cinnamomi, often obtains good effect, visits its reason, yang deficiency can not steam Tianjin
Liquid, thus thirsty, and yang deficiency is unable to astringency inducing body fluid, therefore polyuria, therefore, it is effective that Cortex Cinnamomi is applied to treatment type 2 diabetes mellitus.Additionally, Cortex Cinnamomi
Also there is analgesia, antibacterial and antitumor action, to digestive system, cardiovascular system and immune system, all there is good curative effect,
This comprehensive treating diabetes and its complication can provide theoretical basiss for Cortex Cinnamomi.
Brief description
Fig. 1 is the water extracting method flow chart of cinnaman provided in an embodiment of the present invention.
Fig. 2 is the impact schematic diagram to yield for the Extracting temperature provided in an embodiment of the present invention.
Fig. 3 is the impact schematic diagram to yield for the extraction time provided in an embodiment of the present invention.
Fig. 4 is the impact schematic diagram to yield for the solid-liquid ratio provided in an embodiment of the present invention.
Fig. 5 is the impact schematic diagram to yield for the pH value provided in an embodiment of the present invention.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Below in conjunction with the accompanying drawings the application principle of the present invention is explained in detail.
As shown in figure 1, the water extracting method of cinnaman provided in an embodiment of the present invention comprises the following steps：
S101：Cortex Cinnamomi is crushed to 60 mesh, plus petroleum ether backflow defat, solid-liquid ratio 1:20, use hot water extraction after volatilizing solvent；
100 DEG C of Extracting temperature, pH value 5, extraction time 2h；
S102：After filtering, concentrating plus 8 times of volume dehydrated alcohol are precipitated extracting solution, and sucking filtration obtains filtering residue, dries, and uses
Absolute ethanol washing 3 times, vacuum drying, obtain final product cinnaman.
With reference to test, the application principle of the present invention is further described.
L materials and methods
1.1 materials and equipment
Cortex Cinnamomi：Place of production Guangxi, is accredited as canella Cortex Cinnamomi (Cinnamomum cassia through Yichun College professor Chen Wu
Presl dry bark), clean, dry, pulverizing is standby；Glucose control product：Nat'l Pharmaceutical ＆ Biological Products Control Institute；Oil
Ether, concentrated sulphuric acid, phenol：Domestic pure analysis pure.Streptozotocin (STZ, Sigma Co., USA), metformin (Shanghai Fahrenheit pharmacy
Company limited), glucose kit (Bioengineering Research Institute is built up in Nanjing), it is pure that remaining reagent is analysis.Kunming mouse
(male and female dual-purpose, SPF level, body weight 18～22g, Laboratory Animal Science portion of University Of Nanchang provides).
Digital display thermostat water bath：Fuhua Instrument Ltd. of Jintan City of Jiangsu Province；Constant Temp. Oven：Shanghai precision instrument
Instruments and meters company limited；RE-3000 rotary evaporator：Shanghai Yarong Biochemical Instrument Plant；Electronic balance：Beijing Sai Duolisi instrument
System Co., Ltd；Microplate reader stat Fax 2100：Jiangxi China Tech precision instrument company limited
1.2 test method
1.2.1 the preparation of cinnaman
Cortex Cinnamomi is crushed to 60 mesh, plus petroleum ether backflow defat, uses hot water extraction after volatilizing solvent.Extracting solution through filter, dense
After contracting plus 8 times of volume dehydrated alcohol are precipitated, sucking filtration obtains filtering residue, dries, and with absolute ethanol washing 3 times, vacuum drying, obtains final product
Cinnaman.
1.2.2 phenol -- sulphuric acid assay method
Precision weighs glucose control product 30mg respectively, puts in 50mL volumetric flask, adds ultra-pure water to make 0.6mg/ml's
Mixed solution, as reference substance solution.
Accurately draw 1mL5% phenol to mix with 5ml 98% concentrated sulphuric acid, flowing water cools down, accurately draw 60ul comparison respectively
Product solution or sample solution, the developer that 180ul phenol sulfuric acid mixes, mix, boiling water bath heats 25min, reactant liquor cools down, use
Microplate reader measures its absorbance at 490nm.Replace reference substance solution with 1mL distilled water, operate as blank solution with method.
Calculating regression equation is Y=3.15X-0.003 (R2=0.997)
1.2.3 the assay of cinnaman extract
Take cinnaman extract solution 1ml, measure its absorbance by 1.2.2 methods described.Calculate cinnaman
Content.
1.2.4 experiment of single factor and orthogonal experiment
With the yield of cinnaman as index, factor is investigated for main with Extracting temperature, extraction time, solid-liquid ratio, pH value
Carry out experiment of single factor and orthogonal experiment, Optimized extraction techniques parameter.
1.2.5 blood sugar lowering experiment
After mice adaptability raises 3d, weigh, water 12h is can't help in fasting.10 are taken to be only used as blank control group at random.Remaining is little
By 150mg/kg dosage lumbar injection streptozotocin (STZ), 1 time (STZ is dissolved in 0.1mol/L, the citric acid/Fructus Citri Limoniae of pH4.2 to Mus
In sour sodium buffer, now with the current, ice bath preserves).The above-mentioned buffer of blank control group mouse peritoneal injection equivalent.Recover diet
Weigh after 72h, water 12h docking blood sampling is can't help in fasting, blood is centrifuged 5min in centrifuge 7000r/min, separates serum, use Fructus Vitis viniferae
Sugared kit measurement mice fasting glucose (FBG), all FBG >=11.1mmol/L are considered as diabetic mouse model.Mould mice is become to press
Modeling success mice is divided into 3 groups by blood glucose value homeostatic principle, every group l0, i.e. model group, positive controls (metformin), meat
Osmanthus polysaccharide group.Start within 2nd day daily gavage relative medicine, blank control group and model control group all give equal-volume distilled water,
Successive administration 7 days.Survey weekly a fasting blood sugar.
2 results and analysis
The impact of 2.1 Extracting temperature
Using solid-liquid ratio 1:10, extraction time 2h, the condition of natural pH is extracted to cinnaman, investigates different extraction
The impact result to yield for the temperature is shown in Fig. 2.
From Figure 2 it can be seen that with the raising of Cortex Cinnamomi total polysaccharidess Extracting temperature, rising occurs in the yield of Cortex Cinnamomi total polysaccharidess, and works as temperature
After degree reaches more than 80 DEG C, improving the no significant change of temperature polysaccharide yield, therefore selecting 80 DEG C in operation as total
The Extracting temperature of polysaccharide.
The impact of 2.2 extraction times
Using solid-liquid ratio 1:10,80 DEG C of Extracting temperature, the condition of natural pH is extracted to cinnaman, investigates difference and carries
Take the impact to yield for the time, result is shown in Fig. 3.
From the figure 3, it may be seen that with the prolongation of extraction time, extraction effect slowly raises, when extraction time reaches 120min,
Extraction effect reaches maximum, reduces on the contrary extending time extraction effect, therefore selects extraction time to be 120min.
The impact of 2.3 solid-liquid ratios
Using 80 DEG C of Extracting temperature, extraction time 2h, the condition of natural pH is extracted to cinnaman, investigates different material
The impact to yield for the liquor ratio, result is shown in Fig. 4.
From fig. 4, it can be seen that with the increase of solid-liquid ratio, extraction effect constantly increases, but when solid-liquid ratio is more than 1:When 20, Cortex Cinnamomi
Total polysaccharidess yield increase is less, considering cost selecting factors 1:20 as the optimal solid-liquid ratio extracting.
The impact of 2.4pH value
Using solid-liquid ratio 1:10,80 DEG C of Extracting temperature, extraction time 2h, different pH condition carries to cinnaman
Take, investigate the different impacts to yield for the pH value, result is shown in Fig. 5.
As seen from Figure 5, in the range of solution ph 3-8, the rising Cortex Cinnamomi total polysaccharidess yield with pH value is gradually increasing,
Reach highest during pH6, hereafter change less, therefore optimum pH is 6.
2.5 Orthogonal Experiment and Design
According to single factor experiment result, selection and withdrawal temperature, extraction time, solid-liquid ratio are investigation factor, respectively take 3 water
Flat, from L9(33) orthogonal table tested.With Cortex Cinnamomi total polysaccharidess yield as index, experiment Cortex Cinnamomi powder consumption is 5g every time.Orthogonal
Test level factor is shown in Table 1, and result of the test is shown in Table 2.
Table 1 orthogonal test factor level table
Table 2 L9(34) Orthogonal Experiment and Design and result
As shown in Table 2,4 factors impact primary and secondary order to total polysaccharidess yield is：A>D>C>B is：Extracting temperature>PH value
>Solid-liquid ratio>Extraction time；Optimal processing parameter is A3D1C2B2, that is,：100 DEG C of Extracting temperature, pH5.0, solid-liquid ratio is 1:20, carry
Take time 2h.Experience confirmatory test shows：Amount of water is 20 times of Cortex Cinnamomi powder, pH5.0,100 DEG C of Extracting temperature, extraction time 2h,
Carry out 3 groups, the average yield recording Cortex Cinnamomi total polysaccharidess is 1.783%, and its RSD is 2.57%, and the repeatability of optimum condition is good,
In solid content, total polysaccharidess content is 41.3%.
2.6 variance analyses
Shown by table 3 the results of analysis of variance：Understand that each factors on test result effect is Extracting temperature by F ratio>
PH value>Solid-liquid ratio>Extraction time, that is, affect maximum is Extracting temperature, next to that pH value, solid-liquid ratio, what impact was minimum is to carry
Take the time, this is consistent with interpretation of result above.
Table 3 variance analyses
The impact to blood glucose in diabetic mice for 2.7 cinnamans
After mice adaptability raises 3d, weigh, water 12h is can't help in fasting.10 are taken to be only used as blank control group at random.Remaining is little
By 150mg/kg dosage lumbar injection streptozotocin (STZ), 1 time (STZ is dissolved in 0.1mol/L, the citric acid/Fructus Citri Limoniae of pH4.2 to Mus
In sour sodium buffer, now with the current, ice bath preserves).The above-mentioned buffer of blank control group mouse peritoneal injection equivalent.Recover diet
Weigh after 72h, water 12h docking blood sampling is can't help in fasting, blood is centrifuged 5min in centrifuge 7000r/min, separates serum, use Fructus Vitis viniferae
Sugared kit measurement mice fasting glucose (FBG), all FBG >=11.1mmol/L are considered as diabetic mouse model.Mould mice is become to press
Modeling success mice is divided into 3 groups by blood glucose value homeostatic principle, every group l0, i.e. model group, positive controls (metformin), meat
Osmanthus polysaccharide group.Start within 2nd day daily gavage relative medicine, blank control group and model control group all give equal-volume distilled water,
Cinnaman group fills the cinnaman of 300mg/kg/d, successive administration 7 days.Survey fasting blood sugar and the results are shown in Table 4.
The impact to diabetic mice fasting glucose for table 4 cinnaman
As shown in Table 4, the blood glucose of blank control group mice maintains in normal range always, and model control group mice
Blood glucose value keeps higher level, and before and after the administration of cinnaman group, there were significant differences (P ＜ 0.05) for mouse blood sugar value.Show that Cortex Cinnamomi is many
Sugar can reduce the blood glucose value of diabetic mice.
3 discussion
This test determines the optimization extraction conditions of cinnaman and optimizes precipitate with ethanol condition.Optimized Extraction Process condition is to carry
Take 100 DEG C of temperature, pH value 5, extraction time 2h, solid-liquid ratio 1:20；Wherein Extracting temperature has a significant impact to yield.Optimum condition
Repeatability is good, using industrialized production.There is the blood glucose reducing diabetic mice using the Cortex Cinnamomi total polysaccharidess that this technique is extracted
Effect, the medical value for developing Cortex Cinnamomi further provides theoretical basiss.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (4)
1. a kind of water extracting method of cinnaman is it is characterised in that the solid-liquid ratio 1 of the water extracting method of described cinnaman:
20,100 DEG C of Extracting temperature, pH value 5, extraction time 2h.
2. the water extracting method of cinnaman as claimed in claim 1 is it is characterised in that the water extraction side of described cinnaman
Method comprises the following steps：
Step one, Cortex Cinnamomi is crushed to 60 mesh, plus petroleum ether backflow defat, solid-liquid ratio 1:20, use hot water extraction after volatilizing solvent；Carry
Take 100 DEG C of temperature, pH value 5, extraction time 2h；
Step 2, after filtering, concentrating plus 8 times of volume dehydrated alcohol are precipitated extracting solution, and sucking filtration obtains filtering residue, dries, with no
Water-ethanol washs 3 times, vacuum drying, obtains final product cinnaman.
3. the cinnaman of the extraction of water extracting method of cinnaman described in a kind of utilization claim 1～2 any one.
4. the treatment Rezulin of the water extracting method preparation of cinnaman described in a kind of utilization claim 1～2 any one
Thing.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106957826A (en) * | 2017-03-14 | 2017-07-18 | 昆药集团股份有限公司 | A kind of virus inactivator and its application |
| CN107188984A (en) * | 2017-07-10 | 2017-09-22 | 无限极（中国）有限公司 | A kind of polysaccharide composition and its preparation method and application |
| CN113480673A (en) * | 2021-08-14 | 2021-10-08 | 广西庚源香料有限责任公司 | Method for extracting cinnamon polysaccharide and cinnamon polyphenol from cinnamon branch and leaf oil extraction residues |
| CN113598228A (en) * | 2021-08-05 | 2021-11-05 | 广西庚源香料有限责任公司 | Cinnamon low-sugar yogurt and preparation method thereof |
| CN113667033A (en) * | 2021-09-10 | 2021-11-19 | 中国林业科学研究院林产化学工业研究所 | Cinnamon homogeneous polysaccharide and application thereof in reducing blood sugar |
| CN116622003A (en) * | 2023-07-03 | 2023-08-22 | 北京中医药大学 | Discovery preparation of traditional Chinese medicine material basis and application of traditional Chinese medicine material basis in reducing blood glucose and blood lipid |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106957826A (en) * | 2017-03-14 | 2017-07-18 | 昆药集团股份有限公司 | A kind of virus inactivator and its application |
| CN106957826B (en) * | 2017-03-14 | 2020-02-07 | 昆药集团股份有限公司 | Virus inactivating agent and application thereof |
| CN107188984A (en) * | 2017-07-10 | 2017-09-22 | 无限极（中国）有限公司 | A kind of polysaccharide composition and its preparation method and application |
| CN107188984B (en) * | 2017-07-10 | 2020-03-10 | 无限极（中国）有限公司 | Polysaccharide composition and preparation method and application thereof |
| CN113598228A (en) * | 2021-08-05 | 2021-11-05 | 广西庚源香料有限责任公司 | Cinnamon low-sugar yogurt and preparation method thereof |
| CN113480673A (en) * | 2021-08-14 | 2021-10-08 | 广西庚源香料有限责任公司 | Method for extracting cinnamon polysaccharide and cinnamon polyphenol from cinnamon branch and leaf oil extraction residues |
| CN113667033A (en) * | 2021-09-10 | 2021-11-19 | 中国林业科学研究院林产化学工业研究所 | Cinnamon homogeneous polysaccharide and application thereof in reducing blood sugar |
| CN116622003A (en) * | 2023-07-03 | 2023-08-22 | 北京中医药大学 | Discovery preparation of traditional Chinese medicine material basis and application of traditional Chinese medicine material basis in reducing blood glucose and blood lipid |
| CN116622003B (en) * | 2023-07-03 | 2024-04-05 | 北京中医药大学 | Discovery preparation of traditional Chinese medicine material basis and application of traditional Chinese medicine material basis in reducing blood glucose and blood lipid |
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Application publication date: 20170215 |