CN106391160B - Biochip carrier - Google Patents
Biochip carrier Download PDFInfo
- Publication number
- CN106391160B CN106391160B CN201510822276.2A CN201510822276A CN106391160B CN 106391160 B CN106391160 B CN 106391160B CN 201510822276 A CN201510822276 A CN 201510822276A CN 106391160 B CN106391160 B CN 106391160B
- Authority
- CN
- China
- Prior art keywords
- carrier
- ontology
- hole
- chip
- upper cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000018 DNA microarray Methods 0.000 title claims description 18
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 239000013078 crystal Substances 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 230000000452 restraining effect Effects 0.000 claims description 10
- 238000000465 moulding Methods 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 abstract description 9
- 230000003287 optical effect Effects 0.000 abstract description 5
- 238000005406 washing Methods 0.000 abstract description 3
- 238000004891 communication Methods 0.000 description 14
- 238000011010 flushing procedure Methods 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 9
- 239000002105 nanoparticle Substances 0.000 description 9
- 238000004140 cleaning Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- 239000002082 metal nanoparticle Substances 0.000 description 4
- 230000003595 spectral effect Effects 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- 239000004425 Makrolon Substances 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000005672 electromagnetic field Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A biological chip carrier, form a through hole on a carrier body, form a annular sidewall in the through hole, the inside of the annular sidewall forms a light path channel, connect through at least a junction between the through hole and the annular sidewall, keep a unblocked interval and form a liquid circulation department between the through hole and the annular sidewall; a chip bearing part which is positioned at one end of the annular side wall of the light path channel, and a containing space is formed between the chip bearing part and the upper surface of the body; a biochemical sensing chip is disposed on the chip carrying portion. The carrier can be used for continuously washing the wafer by sufficient washing liquid at one time, the wafer can be sufficiently dried in a drying procedure without liquid residue, light of visible light irradiates through the optical path channel and penetrates out of the wafer, and naked eyes or a detector can observe or receive light signals emitted from the wafer through the containing space.
Description
Technical field
A kind of carrier the present invention relates to carrying biochip to be sensed.
Background technology
Biochip refers to principles such as molecular biology, analytical chemistry, biochemical reactions, with silicon wafer, glass or height
Molecule is base material, designs and make the life with microminiaturization, fast throughput in conjunction with micro- long-pending power technology and biomedical technology
Object and medical detecting element can quickly carry out a large amount of biochemical sensings or reaction in small area, detected to execute medical treatment,
The accurate microminiaturization equipment of the purposes such as environment measuring, food inspection, new drug development, basic research, military defense, chemical synthesis.
Biochemical sensing chip belongs to a type in biochip, for the qualitative of a determinand and quantifies, this is to be measured
Object can be selected from the group that protein, cell, compound, metal ion and combinations thereof are formed.The chip substantially include a base material,
A nano-particle unit and the sensing unit being respectively arranged on the base material.It, should made by the material of the base material by light-permeable
Nano-particle unit includes the most metal nanoparticles for being formed in one surface of base material at intervals, which includes
Most are modified on the substrate, and the reception between such metal nanoparticle and adjacent to such metal nanoparticle
Device.When the receiver of the sensing unit forms specificity with target analytes to be engaged, the locality of metal nanoparticle induction
Electromagnetic field can be influenced by context and be changed, and spectral signal is caused to change, and can thereby detect target analytes.
Biological sensing chip sensing process need to contact with the liquid containing determinand, carries out specificity be bonded react, repeatedly
Cleaning and final drying program, sensing result can be read out by external spectrometer.In sensing process and spectral results
When reading, biological sensing chip should be supported by a carrier.
TaiWan, China new patent M488629 (hereinafter referred to as 629 cases) discloses a kind of carrier of carrying biological sensing chip,
The carrier is apertured member, and there is apertured member a hole, the one end in hole to be equipped with a slot, and chip is set in the slot and is located at the hole
One end, the fluid drip containing determinand is in Kong Zhongyu wafer surface contacts.629 case specifications are it is further noted that this can be loaded and unloaded
The connection type of chip and the apertured member can be gluing, rivet, is spirally connected, welding, rabbeting and be hinged, which is fixed in this
The one end in hole.
As described above, biological sensing chip is needed during the sensing process by cleaning repeatedly, the carrier that 629 cases are taken off is in crystalline substance
In piece cleaning process, needs first to remove the liquid containing determinand from hole, then, flushing liquor is added in hole, later, will rinsed
Liquid removes.Therefore the carrier of 629 cases needs to implement repeatedly the addition of liquid for several times and the action removed and may finally have moisture content
Remain on hole, therefore the spectrum detecting suitable for being not required to drying condition, such as enzyme linked immunosorbent absorption method plate reading (ELISA
Reader), need drying mode local surfaces plasma resonant (localized surface plasmon resonance,
LSPR it) may be decreased interpretation sensitivity.
Invention content
The present invention proposes that a kind of biochip carrier, carrier of the present invention improve the prior art with disposable flushing action
Carrier needed in cleaning process repeatedly adding liquid and remove liquid complexity；On the other hand, carrier of the present invention can pass through
Drying program makes chip obtain the drying for filling part, compared with the spectral detection that the carrier of the prior art is more suitable for drying mode.
The biochip carrier of the present invention, including：
One carrier ontology；
One penetrates through the carrier ontology through-hole upper and lower surfaces of；
The annular sidewall being formed in inside the through-hole, the annular sidewall have a spacing, the annular with the through-hole wall
The outer wall of side wall is connect with the inner wall of the through-hole with an interconnecting piece, which keeps the unimpeded of the spacing to form a liquid according to this
Throughput；It is a path channels within the annular sidewall；
One crystal chip bearing portion is located at one end of the annular sidewall of the path channels；The height in the crystal chip bearing portion is less than should
The upper surface of carrier ontology；The crystal chip bearing portion holds load space between the body upper surface for one；It is set in the crystal chip bearing portion
Set a biochemical sensing chip (hereinafter referred to as chip).
Preferably, above-mentioned through-hole, annular sidewall, interconnecting piece, liquid communication portion, path channels, crystal chip bearing portion and appearance
It carries space and constitutes a load bearing unit；One load bearing unit is set on a carrier ontology.
Preferably, above-mentioned through-hole, annular sidewall, interconnecting piece, liquid communication portion, path channels, crystal chip bearing portion and appearance
It carries space and constitutes a load bearing unit；Most load bearing units are set on a carrier ontology.
Preferably, above-mentioned through-hole, annular sidewall, interconnecting piece, liquid communication portion, path channels, crystal chip bearing portion and appearance
It carries space and constitutes a load bearing unit；1~384 load bearing unit is set on a carrier ontology, which arranges for matrix
Row.
Preferably, the carrier ontology and load bearing unit thereon are made of by integral molding techniques.
Preferably, the carrier ontology and load bearing unit thereon are made of plastic material.
Biological sensing chip needs to contact with the liquid containing determinand during the sensing process, carries out specificity bond reaction, contains
The liquid of determinand carries space by the appearance and instils in the wafer surface, fills part with the wafer surface and contacts, with the scheduled time
Carry out bond reaction；Later, the extra liquid containing determinand need to be rinsed with flushing liquor, the flushing liquor of sufficient quantity is carried by the appearance
Liquid containing determinand is taken away the chip by space and liquid communication portion together；Cleaning procedure complete visual detection need into
Row drying program or without drying program because of the liquid communication portion, path channels and holds in drying program and carries space and make
Chip can get the drying for filling part, no liquid residual.The light of one spectrometer can be irradiated by the path channels and by the crystalline substance
Piece, naked eyes or survey meter can be carried space identification by the appearance or collect the optical signal of the chip.
Above-mentioned carrier further includes a upper cover, which includes：One upper cover ontology；It is formed in the upper cover ontology lower surface
Annular restraining portion；The perforation of one perforation annular restraining portion and the upper cover ontology.
Surface is covered on relative to the mode of the carrier body upper surface on the carrier below the upper cover ontology, annular limit
Portion processed stretches into the appearance and carries space, surrounds and be limited to the periphery of the chip.
Preferably, above-mentioned annular restraining portion and perforation constitute a limiting unit, it is arranged 1 on a upper cover ontology~
384 limiting units, the limiting unit are matrix arrangement.
Preferably, the upper cover ontology and limiting unit thereon are made of by integral molding techniques.
Preferably, the upper cover ontology and limiting unit thereon are made of plastic material.
Biological sensing chip needs to contact with the liquid containing determinand during sensing, carries out specificity bond reaction, contains
The liquid of determinand is instiled in the wafer surface by the perforation of the lid ontology or is riddled in entire perforation, containing to be measured
The liquid of object fills part with the wafer surface and contacts, and bond reaction is carried out with the scheduled time；Later, the upper cover is removed, then with punching
Washing lotion rinses the extra liquid containing determinand, and the flushing liquor of sufficient quantity carries space and liquid communication portion by the appearance, will contain
The liquid of determinand takes away the chip together；Cleaning procedure completes visual detection and needs program to be dried or without dry journey
Sequence makes the available drying for filling part of chip, nothing in drying program because the liquid communication portion, path channels and appearance carry space
Liquid remains.The light of one spectrometer can be irradiated by the path channels and by the chip, naked eyes or survey meter can be by these
Hold the optical signal for carrying space identification or collecting the chip.
The beneficial effects of the present invention are：
The carrier of the present invention is persistently rinsed chip for disposable sufficient flushing liquor, without executing addition repeatedly
Liquid and the action for removing liquid.
The carrier of the present invention can make chip obtain the drying for filling part by drying program, more applicable compared with the carrier of the prior art
In the spectral detection of drying mode, such as surface plasma resonant (localized surface plasmon resonance,
LSPR), and promote sensitivity.
Description of the drawings
Fig. 1 is chip carrier front appearance of the present invention and internal perspective view.
Fig. 2 is the 2-2 sectional views of Fig. 1.
Fig. 3 is the outside drawing of array chip carrier of the present invention.
Fig. 4 is that the chip carrier of the present invention carries a chip and carries out the schematic diagram of sensing step.
Fig. 5 is the outside drawing of the upper cover of chip carrier of the present invention.
Fig. 6 is the 6-6 sectional views of Fig. 5.
Fig. 7 is the outside drawing of array upper cover of the present invention.
Fig. 8 is the combination section of chip carrier of the present invention and upper cover.
Fig. 9 is that the chip carrier of the present invention carries a chip with upper cover and carries out the schematic diagram of sensing step.
Reference numeral
10：Carrier ontology；11：Through-hole；12：Annular sidewall；13：Liquid communication portion；14：Interconnecting piece；15：Path channels；
16：Crystal chip bearing portion；17：Hold and carries space；20：Biochemical sensing chip；31：Liquid containing determinand；32：Flushing liquor；33：Light
Line；40：Upper cover；41：Upper cover ontology；42：Lower surface；43：Annular restraining portion；44：Perforation；100：Load bearing unit；101：Upper table
Face；102：Lower surface；400：Limiting unit.
Specific implementation mode
For ease of illustration central idea represented in one column of foregoing invention content, is hereby expressed with specific embodiment.Implement
Various different object systems are described in suitable for the ratio of explanation, size, deflection or displacement in example, rather than by actual components
Ratio is drawn, and conjunction is first chatted bright.
Such as Fig. 1 to Fig. 3, biochip carrier of the invention, including：
One carrier ontology 10；
One penetrates through the through-hole 11 of 10 upper surface 101 of carrier ontology and lower surface 102；The section configuration of the through-hole can be circle
Shape, ellipse, polygon, polygonal or other various geometries；
The annular sidewall 12 being formed in inside the through-hole 11, the annular sidewall 12 and 11 inner wall of through-hole have between one
Away from the outer wall of the annular sidewall 12 is connect with the inner wall of the through-hole 11 with an at least interconnecting piece 14, which keeps between being somebody's turn to do
Away from unimpeded form a liquid communication portion 13 according to this；It is a path channels 15 within the annular sidewall 12；The annular sidewall 12
Section configuration can be circle, ellipse, polygon, polygonal or other various geometries；
One crystal chip bearing portion 16 is located at one end of the annular sidewall 12；The height in the crystal chip bearing portion 16 is less than the carrier
The upper surface 101 of ontology 10；The crystal chip bearing portion 16 holds load space 17 between 10 upper surface 101 of carrier ontology for one；It should
With can technological means (including bonding) one biochemical sensing chip 20 (hereinafter referred to as chip) of setting for unloading of group in crystal chip bearing portion 16.
The area of the chip 20 is 1~2500mm2, for example, can be (1~43mm) × (1~43 mm).The chip
The group that 20 contour shape can be formed for circle, ellipse, polygon, irregular shape and combinations thereof.The chip 20 includes
One base material, a nano-particle unit and a sensing unit, the wherein base material are the nano-particles with made by a light-transmitting materials
Unit is set on the base material, and includes a plurality of nano-particles separately, respectively the nano-particle made by a metal,
The grain size of the nano-particle is 1~200nm, and it is 1~200nm, the chip 20 to have a spacing, the spacing between the nano-particle
Further include a plurality of receivers between being set to those nano-particles.
Through-hole 11, annular sidewall 12, liquid communication portion 13, path channels 15, crystal chip bearing portion 16 and the Rong Zaikong
Between 17 be defined as a load bearing unit 100 in the present invention.Such as Fig. 1, a carrying can be set on a carrier ontology 10
Most load bearing units 100 can be arranged, and load bearing unit 100 is arranged in arrays such as Fig. 3 in unit 100 in the carrier body 10.
In other words, load bearing unit number 100 set on the carrier ontology 10 can be the integer between 1~384.The carrier ontology
10 and load bearing unit 100 thereon can be made up of integral molding techniques, the material of carrier ontology 10 is including but not limited to poly- third
Alkene (PP), makrolon (PC), polyamide (Nylon).
Such as Fig. 4, chip 20 needs to contact with the liquid containing determinand during the sensing process, carries out specificity bond reaction, should
Determinand is selected from the group being made of a protein, a cell, a compound, a metal ion and combinations thereof.Containing determinand
Liquid 31 by the appearance carry space 17 instil in 20 surface of chip, fill part with the wafer surface and contact, and with it is scheduled when
Between carry out bond reaction (such as Fig. 4 (B))；Later, the extra liquid 31 containing determinand, sufficient quantity need to be rinsed with flushing liquor 32
Flushing liquor 32 space 17 and liquid communication portion 13 are carried by the appearance, the liquid 31 containing determinand is taken away into the chip 20 together
And carrier ontology 10 (such as Fig. 4 (C)).Cleaning procedure completes visual detection and needs to be dried program or without drying program,
In drying program, because of the liquid communication portion 13, path channels 15 and holding load space 17 makes chip 20 is available to fill the dry of part
Dry, no liquid remains (such as Fig. 4 (D)).The light 33 of one visible light can be irradiated by the path channels 15 and by the chip 20,
Naked eyes or spectrometer can be carried the identification of space 17 by the appearance or collect the optical signal (such as Fig. 4 (E)) of the chip.
Such as Fig. 5 to Fig. 8, above-mentioned carrier further includes a upper cover 40, which includes：One upper cover ontology 41；It is formed in
The annular restraining portion 43 of 41 lower surface 42 of upper cover ontology；One penetrates through the perforation of the annular restraining portion 43 and the upper cover ontology 41
44。
41 or less the surface 42 of upper cover ontology covers on the carrier sheet relative to the mode of 10 upper surface 101 of carrier ontology
On body 10, which stretches into the appearance and carries space 17, surrounds and be limited to the periphery of the chip 20.
The annular restraining portion 43 and perforation 44 is defined as a limiting unit, such as Fig. 5, at one in the present invention
One limiting unit 400 can be set on upper cover ontology 41, such as Fig. 7, most limiting units can be set on the upper cover ontology 41
400, and limiting unit 400 is arranged in arrays.In other words, 400 number of limiting unit set on the upper cover ontology 41 can be 1~
Integer between 384.The upper cover ontology 41 and limiting unit 400 thereon can be made up of integral molding techniques, upper cover sheet
The material of body 41 is including but not limited to polypropylene (PP), makrolon (PC), polyamide (Nylon).
Such as Fig. 9, chip 20 needs to contact with the liquid 31 containing determinand during the sensing process, carries out specificity bond reaction,
Liquid 31 containing determinand is instiled in 20 surface of chip by perforation 44 of the upper cover ontology 41 or is riddled entire
In perforation 44 (such as Fig. 9 (B)), the liquid 31 containing determinand fills part with 20 surface of chip and contacts, with the scheduled time into line unit
Knot reaction；Later, the upper cover 40 (such as Fig. 9 (C)) is removed, then the extra liquid 31 containing determinand is rinsed with flushing liquor 32, is filled
Enough flushing liquors carry space 17 and liquid communication portion 13 by the appearance, and the liquid 31 containing determinand is taken away the chip together
20 and carrier ontology 10 (such as Fig. 9 (D))；Cleaning procedure completes visual detection and needs program to be dried or without dry journey
Sequence, in drying program, because of the liquid communication portion 13, path channels 15 and holding load space 17 makes chip 20 is available to fill part
Dry, no liquid remains (such as Fig. 9 (E)).The light 33 of one visible light can be irradiated by the path channels 15 and pass through the chip
20, naked eyes or spectrometer can be carried the identification of space 17 by the appearance or collect the optical signal (such as Fig. 9 (F)) of the chip.
Claims (10)
1. a kind of biochip carrier, which is characterized in that including：
One carrier ontology；
One penetrates through the through-hole of the upper surface and the lower surface of the carrier ontology；
The annular sidewall being formed in inside the through-hole, the annular sidewall have a spacing, the annular sidewall with the through-hole wall
Outer wall connect with an at least interconnecting piece with the inner wall of the through-hole, which keeps the unimpeded of the spacing to form a liquid according to this
Throughput；It is a path channels within the annular sidewall；
One crystal chip bearing portion is located at one end of the annular sidewall；The height in the crystal chip bearing portion is less than the upper table of the carrier ontology
Face；The crystal chip bearing portion holds load space between the carrier body upper surface for one；With can the skill unloaded of group in the crystal chip bearing portion
A biochemical sensing chip is arranged in art means.
2. biochip carrier as described in claim 1, which is characterized in that above-mentioned through-hole, annular sidewall, interconnecting piece, liquid
Body throughput, path channels, crystal chip bearing portion and appearance carry space and constitute a load bearing unit；It is arranged one on a carrier ontology
A load bearing unit.
3. biochip carrier as described in claim 1, which is characterized in that above-mentioned through-hole, annular sidewall, interconnecting piece, liquid
Body throughput, path channels, crystal chip bearing portion and appearance carry space and constitute a load bearing unit；It is arranged on a carrier ontology more
Several load bearing units.
4. biochip carrier as described in claim 1, which is characterized in that above-mentioned through-hole, annular sidewall, interconnecting piece, liquid
Body throughput, path channels, crystal chip bearing portion and appearance carry space and constitute a load bearing unit；On a carrier ontology setting 1~
384 load bearing units, the load bearing unit are matrix arrangement.
5. the biochip carrier as described in any of which item of claim 2 to 4, which is characterized in that the carrier ontology and
Load bearing unit thereon is made of by integral molding techniques.
6. the biochip carrier as described in the one of which of claim 2 to 4, which is characterized in that the carrier ontology and its
On load bearing unit be made of plastic material.
7. biochip carrier as described in claim 1, which is characterized in that further include a upper cover, which includes a upper cover
Ontology, the annular restraining portion for being formed in the upper cover ontology lower surface；One perforation annular restraining portion is passed through with the upper cover ontology
Hole；Surface below the upper cover ontology is covered on relative to the carrier body upper surface on the carrier ontology, which stretches
Enter the appearance and carry space, surrounds and be limited to the periphery of the chip.
8. biochip carrier as claimed in claim 7, which is characterized in that above-mentioned annular restraining portion and perforation constitute a limit
Unit processed, is arranged 1~384 limiting unit on a upper cover ontology, which is matrix arrangement.
9. biochip carrier as claimed in claim 8, which is characterized in that the upper cover ontology and limiting unit thereon are
It is made up of integral molding techniques.
10. biochip carrier as claimed in claim 9, which is characterized in that the upper cover ontology and limiting unit thereon
It is made of plastic material.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW104124605 | 2015-07-29 | ||
| TW104124605A TWI595223B (en) | 2015-07-29 | 2015-07-29 | Bio-chip carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106391160A CN106391160A (en) | 2017-02-15 |
| CN106391160B true CN106391160B (en) | 2018-07-31 |
Family
ID=58007783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510822276.2A Expired - Fee Related CN106391160B (en) | 2015-07-29 | 2015-11-24 | Biochip carrier |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN106391160B (en) |
| TW (1) | TWI595223B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201111782A (en) * | 2009-09-16 | 2011-04-01 | Nat Univ Tsing Hua | Anesthetic sensing optical microfluidic chip system |
| CN103341370A (en) * | 2008-10-28 | 2013-10-09 | 藤仓化成株式会社 | Liquid passage device and manufacturing method |
| TW201341004A (en) * | 2012-04-12 | 2013-10-16 | Univ Nat Cheng Kung | Device for liquid sample filtration |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2731423B2 (en) * | 1989-06-30 | 1998-03-25 | 日本商事株式会社 | Rotary cuvette |
| US6794981B2 (en) * | 1998-12-07 | 2004-09-21 | Honeywell International Inc. | Integratable-fluid flow and property microsensor assembly |
| TWM488629U (en) * | 2014-01-22 | 2014-10-21 | Siward Crystal Technology Co Ltd | Sensing device |
-
2015
- 2015-07-29 TW TW104124605A patent/TWI595223B/en not_active IP Right Cessation
- 2015-11-24 CN CN201510822276.2A patent/CN106391160B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103341370A (en) * | 2008-10-28 | 2013-10-09 | 藤仓化成株式会社 | Liquid passage device and manufacturing method |
| TW201111782A (en) * | 2009-09-16 | 2011-04-01 | Nat Univ Tsing Hua | Anesthetic sensing optical microfluidic chip system |
| TW201341004A (en) * | 2012-04-12 | 2013-10-16 | Univ Nat Cheng Kung | Device for liquid sample filtration |
Also Published As
| Publication number | Publication date |
|---|---|
| TWI595223B (en) | 2017-08-11 |
| TW201704731A (en) | 2017-02-01 |
| CN106391160A (en) | 2017-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101354764B1 (en) | Various substances supporter, various substances supporter processing apparatus, and method of mentioned processing | |
| US7803322B2 (en) | Universal optical imaging and processing system | |
| US20020110817A1 (en) | Device for containing, reacting and measuring, and method of containing, reacting and measuring | |
| CN106232233B (en) | Sample collection unit | |
| CN104042253B (en) | Rotatable dish type fluid sample collection device | |
| US7662345B2 (en) | Fluid handling apparatus and fluid handling unit for use therein | |
| US20120261256A1 (en) | Sample holders and analytical instrument for point-of-care qualification of clinical samples | |
| CN107073470A (en) | Point-of care analysis process system | |
| CN105874053A (en) | Multi-well cuvette provided with integrated reaction and detection means | |
| KR20180031612A (en) | A Method of Rapid Diagnosis With High Sensitivity By Using Single Diagnosis Chip Comprising Reaction and Analysis Process | |
| CN103079706A (en) | Pipette tip, pipette system and method for performing analysis with the pipette tip and system | |
| EP3615921A2 (en) | Orthogonal polybiosensing and imaging systems | |
| CN109073561A (en) | Detection of Organic Chemicals | |
| CN101629952A (en) | Single column immunological test elements | |
| US20160223534A1 (en) | Device and method for detecting, while using a microfluidic chip, the resistance of bacteria to an active substance to be analyzed | |
| JP6851079B2 (en) | Multiple reaction parallel measuring device and its method | |
| AT502549B1 (en) | DEVICE FOR THE ANALYSIS OF LIQUID SAMPLES | |
| US10133978B2 (en) | Device having RFID tag and fluidics element | |
| CN106391160B (en) | Biochip carrier | |
| US20140326598A1 (en) | Module-type biosensor | |
| JP2005508006A (en) | Sample well strip | |
| CN108700608A (en) | Automatic analysis system for in-vitro diagnosis | |
| JP2007205728A (en) | Lid body for reagent container, reagent container on which lid body is mounted and reagent kid | |
| JP2011017607A (en) | Reaction container and automatic analyzer using container | |
| CN112005116B (en) | Substrate for biochemical reaction and analyzer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180731 Termination date: 20191124 |