CN106391160A - biochip carrier - Google Patents
biochip carrier Download PDFInfo
- Publication number
- CN106391160A CN106391160A CN201510822276.2A CN201510822276A CN106391160A CN 106391160 A CN106391160 A CN 106391160A CN 201510822276 A CN201510822276 A CN 201510822276A CN 106391160 A CN106391160 A CN 106391160A
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- Prior art keywords
- carrier
- chip
- hole
- load bearing
- biochip
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- 238000000018 DNA microarray Methods 0.000 title claims description 18
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 239000013078 crystal Substances 0.000 claims description 20
- 238000004891 communication Methods 0.000 claims description 18
- 239000000463 material Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 11
- 230000000452 restraining effect Effects 0.000 claims description 11
- 238000000465 moulding Methods 0.000 claims description 6
- 238000003780 insertion Methods 0.000 claims description 5
- 230000037431 insertion Effects 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 abstract description 11
- 230000003287 optical effect Effects 0.000 abstract description 4
- 238000005406 washing Methods 0.000 abstract description 3
- 238000011010 flushing procedure Methods 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 10
- 238000004140 cleaning Methods 0.000 description 8
- 239000002105 nanoparticle Substances 0.000 description 8
- 238000001514 detection method Methods 0.000 description 7
- 239000004743 Polypropylene Substances 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000002082 metal nanoparticle Substances 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 239000004952 Polyamide Substances 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000005672 electromagnetic field Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A biological chip carrier, form a through hole on a carrier body, form a annular sidewall in the through hole, the inside of the annular sidewall forms a light path channel, connect through at least a junction between the through hole and the annular sidewall, keep a unblocked interval and form a liquid circulation department between the through hole and the annular sidewall; a chip bearing part which is positioned at one end of the annular side wall of the light path channel, and a containing space is formed between the chip bearing part and the upper surface of the body; a biochemical sensing chip is disposed on the chip carrying portion. The carrier can be used for continuously washing the wafer by sufficient washing liquid at one time, the wafer can be sufficiently dried in a drying procedure without liquid residue, light of visible light irradiates through the optical path channel and penetrates out of the wafer, and naked eyes or a detector can observe or receive light signals emitted from the wafer through the containing space.
Description
Technical field
The present invention is with regard to a kind of carrying carrier to be sensed for the biochip.
Background technology
Biochip refers to principles such as molecular biology, analytical chemistry, biochemical reactions, with silicon wafer
Piece, glass or macromolecule are base material, in conjunction with micro- long-pending power technology and biomedical technology design and making
There is the biological and medical detecting element of microminiaturization, fast throughput, can be fast in small area
Speed carries out a large amount of biochemical sensings or reaction, in order to execute medical detection, environment measuring, food inspection,
The accurate microminiaturization equipment of the purposes such as new drug development, basic research, military defense, chemosynthesis.
Biochemical sensing chip belongs to one of biochip type, for the qualitative of a determinand and calmly
Amount, this determinand is selected from what protein, cell, compound, metal ion and combinations thereof were formed
Group.This chip substantially comprise a base material, the nanoparticle unit being respectively arranged on this base material and
One sensing unit.Made by the material of light-permeable, how several this nanoparticle unit includes this base material
It is formed at the metal nanoparticle on this base material one surface at intervals, this sensing unit includes how several repairing
Decorations on the substrate, and are located between the metal nanoparticle such as this and adjacent to the metal nanoparticle such as this
Receptor.When the receptor of this sensing unit forms specificity with target analytes and engages, metal is received
The locality electromagnetic field of rice corpuscles induction can be affected by context and be changed, and derivative spectomstry signal becomes
Change, thereby can detect target analytes.
Biological sensing chip need to be contacted with the liquid containing determinand, carry out specificity bond in sensing process
Reaction, repeatedly cleaning and final drying program, sensing result can be carried out by outside spectrogrph
Read.When the process of sensing and spectral results read, biological sensing chip should be supported by a carrier.
TaiWan, China new patent M488629 (hereinafter referred to as 629 case) discloses a kind of carrying biological sensing
The carrier of chip, this carrier is apertured member, and apertured member has a hole, and the one end in hole is provided with a groove,
Chip is arranged in this groove and is located at the one end in this hole, and the fluid drip containing determinand is in Kong Zhongyu chip
Surface contacts.629 case description it is further noted that this can loading and unloading wafer and this apertured member connection
Mode can be gluing, rivets, is spirally connected, welding, rabbeting and be hinged, and this chip is fixed in this hole
One end.
As described above, biological sensing chip needs through cleaning repeatedly, 629 case institutes during sensing
The carrier taken off, in cleaning process, needs first to remove the liquid containing determinand from hole, then,
Add flushing liquor in hole, afterwards, flushing liquor is removed.The carrier of therefore 629 cases needs repeatedly real
Apply liquid for several times to add and the action that removes and moisture content may finally be had to residue in hole, be therefore applied to
It is not required to the spectrum detecting of drying condition, such as enzyme linked immunosorbent absorption method plate reading (ELISA
Reader), needing local surfaces plasma resonant (the localized surface plasmon of drying mode
Resonance, LSPR) interpretation sensitivity may be reduced.
Content of the invention
The present invention proposes a kind of biochip carrier, and carrier of the present invention is improved with disposable flushing action
The carrier of prior art needs repeatedly adding liquid in cleaning process and removes the complexity of liquid；Separately
On the one hand, carrier of the present invention can make chip obtain the drying filling part by drying program, compared with prior art
Carrier be more suitable for the spectral detection of drying mode.
The biochip carrier of the present invention, including：
One carrier body；
The through hole on this upper and lower surface of carrier body of one insertion；
It is formed in the annular sidewall within this through hole, this annular sidewall and this through-hole wall have between one
Away from the outer wall of this annular sidewall is connected with a connecting portion with the inwall of this through hole, and this connecting portion keeps should
The unimpeded of spacing forms a liquid communication portion according to this；It is path channels within this annular sidewall；
One crystal chip bearing portion, positioned at one end of the annular sidewall of this path channels；This crystal chip bearing portion
Highly it is less than the upper surface of this carrier body；This crystal chip bearing portion is an appearance to this body upper surface
Carry space；One biochemical sensing chip (hereinafter referred to as chip) is arranged on this crystal chip bearing portion.
Preferably, above-mentioned through hole, annular sidewall, linking part, liquid communication portion, path channels,
Crystal chip bearing portion and appearance carry space and constitute a load bearing unit；A carrier body can arrange one
Load bearing unit.
Preferably, above-mentioned through hole, annular sidewall, linking part, liquid communication portion, path channels,
Crystal chip bearing portion and appearance carry space and constitute a load bearing unit；A carrier body can arrange majority
Individual load bearing unit.
Preferably, above-mentioned through hole, annular sidewall, linking part, liquid communication portion, path channels,
Crystal chip bearing portion and appearance carry space and constitute a load bearing unit；A carrier body can arrange 1～
384 load bearing units, this load bearing unit is matrix arrangement.
Preferably, described carrier body and load bearing unit thereon are made of by integral molding techniques.
Preferably, described carrier body and load bearing unit thereon are made up of plastic material.
Biological sensing chip needs to contact with the liquid containing determinand during sensing, carries out specificity key
Knot reaction, the liquid containing determinand carries space by this appearance and instils in this wafer surface, with this chip table
Part contact is filled in face, carries out bond reaction with the predetermined time；Afterwards, need to be unnecessary with flushing liquor flushing
Liquid containing determinand, the flushing liquor of sufficient quantity carries space and liquid communication portion by this appearance, will contain
The liquid of determinand takes away this chip in the lump；Cleaning procedure completes visual detection to be needed to be dried program
Or it is not dried program, in drying program, because this liquid communication portion, path channels and appearance carry
Space makes chip can obtain the drying filling part, and no liquid remains.The light of one spectrogrph can pass through this light
Paths irradiate and pass through this chip, and naked eyes or survey meter can carry space identification by this appearance or collect should
The optical signal of chip.
Above-mentioned carrier further includes lid on, and on this, lid includes：Lid body on one；It is formed in lid on this
The annular restraining portion of body lower surface；This annular restraining portion of one insertion with this on lid body perforation.
On this, lid body covers on this carrier in the way of lower surface is with respect to this carrier body upper surface
On, this annular restraining portion stretches into this appearance and carries space, surrounds and be limited to the periphery of this chip.
Preferably, above-mentioned annular restraining portion and perforation constitute a limiting unit, lid body on
On 1～384 limiting unit can be set, this limiting unit be matrix arrangement.
Preferably, described upper lid body and limiting unit thereon are made of by integral molding techniques.
Preferably, described upper lid body and limiting unit thereon are made up of plastic material.
Biological sensing chip needs to contact with the liquid containing determinand during sensing, carries out specificity key
Knot reaction, the liquid containing determinand instiled by the perforation of this lid body in this wafer surface or
Riddle in whole perforation, the liquid containing determinand fills part with this wafer surface and contacts, with predetermined when
Between carry out bond reaction；Afterwards, take off lid on this, then with flushing liquor rinse unnecessary containing determinand
Liquid, the flushing liquor of sufficient quantity carries space and liquid communication portion by this appearance, by the liquid containing determinand
Body takes away this chip in the lump；Cleaning procedure completes visual detection to be needed to be dried program or is not done
Dry program, in drying program, makes chip because this liquid communication portion, path channels and appearance carry space
The drying filling part can be obtained, no liquid remains.The light of one spectrogrph can be irradiated by this path channels
And passing through this chip, naked eyes or survey meter can carry space identification or the light letter collecting this chip by this appearance
Number.
The beneficial effects of the present invention is：
The carrier of the present invention is available for disposably sufficient flushing liquor and chip is persistently rinsed, need not be anti-
Execution adding liquid and the action removing liquid again.
The carrier of the present invention can make chip obtain the drying filling part by drying program, compared with prior art
Carrier is more suitable for the spectral detection of drying mode, such as surface plasma resonant (localized surface
Plasmon resonance, LSPR), and promote sensitivity.
Brief description
Fig. 1 is chip carrier front appearance of the present invention and perspective internal view.
Fig. 2 is the 2-2 profile of Fig. 1.
Fig. 3 is the outside drawing of array chip carrier of the present invention.
Fig. 4 is that the chip carrier of the present invention carries a chip and carries out sensing the schematic diagram of step.
Fig. 5 is the outside drawing of the upper lid of chip carrier of the present invention.
Fig. 6 is the 6-6 profile of Fig. 5.
Fig. 7 is the outside drawing of lid in array of the present invention.
Fig. 8 is the combination section of chip carrier of the present invention and upper lid.
Fig. 9 is that the chip carrier of the present invention carries a chip with upper lid and carries out sensing the signal of step
Figure.
Reference
10：Carrier body；11：Through hole；12：Annular sidewall；13：Liquid communication portion；14：Even
Socket part；15：Path channels；16：Crystal chip bearing portion；17：Hold and carry space；20：Biochemical sensing is brilliant
Piece；31：Liquid containing determinand；32：Flushing liquor；33：Light；40：Upper lid；41：Upper lid
Body；42：Lower surface；43：Annular restraining portion；44：Perforation；100：Load bearing unit；101：
Upper surface；102：Lower surface；400：Limiting unit.
Specific embodiment
For ease of represented central idea in explanation foregoing invention content one hurdle, hereby with specific embodiment
Expression.In embodiment, various difference object systems are in ratio, size, deflection or the displacement being suitable to explanation
Measure and describe, rather than drawn in the ratio of actual components, conjunction is first chatted bright.
As Fig. 1 to Fig. 3, the biochip carrier of the present invention, including：
One carrier body 10；
This carrier body 10 upper surface 101 of one insertion and the through hole 11 of lower surface 102；The section of this through hole
Shape can be circle, ellipse, polygon, polygon or other various geometries；
It is formed in this annular sidewall 12 within through hole 11, this annular sidewall 12 and this through hole 11 inwall
There is a spacing, the outer wall of this annular sidewall 12 is connected with least one connecting portion 14 with the inwall of this through hole 11
Connect, this connecting portion 14 keeps the unimpeded of this spacing to form a liquid communication portion 13 according to this；This annular sidewall
It is path channels 15 within 12；The section configuration of this annular sidewall 12 can be circular, oval, many
Side shape, polygon or other various geometries；
One crystal chip bearing portion 16, positioned at one end of this annular sidewall 12；The height in this crystal chip bearing portion 16
Upper surface 101 less than this carrier body 10；This crystal chip bearing portion 16 is to this carrier body 10 upper surface
It is that an appearance carries space 17 between 101；Can the technological means unloaded of group (include glutinous in this crystal chip bearing portion 16
Close) setting one biochemical sensing chip 20 (hereinafter referred to as chip).
The area of this chip 20 is 1～2500mm2, for example, can be (1～43mm) × (1～43
mm).The contour shape of this chip 20 can be circle, ellipse, polygon, irregular shape and its group
Close formed group.This chip 20 comprises a base material, a nanoparticle unit and a sensing unit,
Wherein this base material is so that, made by light-transmitting materials, this nanoparticle unit is arranged on this base material,
And comprise a plurality of nanoparticles separately, respectively this nanoparticle made by a metal, this nanometer
The particle diameter of particle is 1～200nm, has a spacing between this nanoparticle, and this spacing is 1～200nm,
This chip 20 further includes a plurality of receptors being arranged between those nanoparticles.
Described through hole 11, annular sidewall 12, liquid communication portion 13, path channels 15, crystal chip bearing
Portion 16 and appearance carry space 17 and are defined as a load bearing unit 100 in the present invention.As Fig. 1, one
One load bearing unit 100 can be arranged on individual carrier body 10, such as Fig. 3, this carrier body 10 can be arranged
How several load bearing unit 100, and load bearing unit 100 is arranged in arrays.In other words, this carrier body 10
Upper set load bearing unit number 100 can be the integer between 1～384.Described carrier body 10 and thereon
Load bearing unit 100 can be made by integral molding techniques, the material of carrier body 10 comprises but does not limit
In polypropylene (PP), Merlon (PC), polyamide (Nylon).
As Fig. 4, chip 20 needs to contact with the liquid containing determinand during sensing, carries out specificity
Bond reaction, this determinand be selected from a protein, a cell, a compound, a metal ion and
The group that a combination thereof is formed.Liquid 31 containing determinand carries space 17 by this appearance and instils in this chip
20 surfaces, fill part with this wafer surface and contact, and carry out bond reaction (as schemed with the predetermined time
4(B))；Afterwards, the unnecessary liquid 31 containing determinand need to be rinsed with flushing liquor 32, the rushing of sufficient quantity
Washing liquid 32 carries space 17 and liquid communication portion 13 by this appearance, and the liquid 31 containing determinand is carried in the lump
From this chip 20 and carrier body 10 (as Fig. 4 (C)).Cleaning procedure completes visual detection to be needed to carry out
Drying program or be not dried program, in drying program, because this liquid communication portion 13, light path are led to
Road 15 and appearance carry space 17 and make chip 20 can obtain the drying filling part, and no liquid remains (as Fig. 4 (D)).
The light 33 of one visible ray can be irradiated and be passed through by this path channels 15 this chip 20, naked eyes or spectrum
Instrument can carry, by this appearance, the optical signal (as Fig. 4 (E)) that space 17 recognized or collected this chip.
As Fig. 5 to Fig. 8, above-mentioned carrier further includes and covers 40 on one, and on this, lid 40 includes：Lid body on one
41；It is formed in the annular restraining portion 43 of lid body 41 lower surface 42 on this；This annular of one insertion limits
Portion 43 with this on lid body 41 perforation 44.
On this, lid body 41 lid in the way of lower surface 42 is with respect to this carrier body 10 upper surface 101 covers
On this carrier body 10, this annular restraining portion 43 stretches into this appearance and carries space 17, surrounds and is limited to this
The periphery of chip 20.
Described annular restraining portion 43 and perforation 44 are defined as a limiting unit, such as in the present invention
Fig. 5, lid body 41 on can arrange a limiting unit 400, such as Fig. 7, lid body on this
How several limiting unit 400 can be set on 41, and limiting unit 400 is arranged in arrays.In other words, should
Set limiting unit 400 number on upper lid body 41 can be the integer between 1～384.Described upper lid is originally
Body 41 and limiting unit thereon 400 can be made by integral molding techniques, the material of upper lid body 41
Including but not limited to polypropylene (PP), Merlon (PC), polyamide (Nylon).
As Fig. 9, chip 20 needs to contact with the liquid 31 containing determinand during sensing, carries out specially
One property bond reaction, the liquid 31 containing determinand is instiled by the perforation 44 of lid body 41 on this
In this chip 20 surface or riddle (as Fig. 4 (B)) in whole perforation 44, containing determinand
Liquid 31 fills part with this chip 20 surface and contacts, and carries out bond reaction with the predetermined time；Afterwards,
Remove lid 40 (as Fig. 4 (C)) on this, the more unnecessary liquid containing determinand is rinsed with flushing liquor 32
31, the flushing liquor of sufficient quantity carries space 17 and liquid communication portion 13 by this appearance, will contain determinand
Liquid 31 take away this chip 20 and carrier body 10 (as Fig. 4 (D)) in the lump；Cleaning procedure completes
Visual detection needs to be dried program or is not dried program, in drying program, because of this liquid
Throughput 13, path channels 15 and appearance carry space 17 makes chip 20 can obtain the drying filling part,
No liquid remains (as Fig. 4 (E)).The light 33 of one visible ray can be irradiated by this path channels 15
And passing through this chip 20, naked eyes or spectrogrph can carry space 17 by this appearance and recognize or collect this chip
Optical signal (as Fig. 4 (F)).
Claims (10)
1. a kind of biochip carrier is it is characterised in that include：
One carrier body；
The upper surface of this carrier body of one insertion and the through hole of lower surface；
It is formed in the annular sidewall within this through hole, this annular sidewall and this through-hole wall have between one
Away from the outer wall of this annular sidewall is connected with least one connecting portion with the inwall of this through hole, and this connecting portion is protected
Hold the unimpeded of this spacing and form a liquid communication portion according to this；It is path channels within this annular sidewall；
One crystal chip bearing portion, positioned at one end of this annular sidewall；The height in this crystal chip bearing portion is less than should
The upper surface of carrier body；This crystal chip bearing portion is that an appearance carries sky to this carrier body upper surface
Between；So that the technological means unloaded of group a biochemical sensing chip can be arranged in this crystal chip bearing portion.
2. biochip carrier as claimed in claim 1 is it is characterised in that above-mentioned through hole, ring
Shape side wall, linking part, liquid communication portion, path channels, crystal chip bearing portion and appearance carry space and constitute
One load bearing unit；One load bearing unit can be arranged on a carrier body.
3. biochip carrier as claimed in claim 1 is it is characterised in that above-mentioned through hole, ring
Shape side wall, linking part, liquid communication portion, path channels, crystal chip bearing portion and appearance carry space and constitute
One load bearing unit；How several load bearing unit can be arranged on a carrier body.
4. biochip carrier as claimed in claim 1 is it is characterised in that above-mentioned through hole, ring
Shape side wall, linking part, liquid communication portion, path channels, crystal chip bearing portion and appearance carry space and constitute
One load bearing unit；1～384 load bearing unit can be arranged on a carrier body, this load bearing unit is
Matrix arrangement.
5. the biochip carrier as described in any of which item of claim 2 to 4 it is characterised in that
Described carrier body and load bearing unit thereon are made of by integral molding techniques.
6. the biochip carrier as described in the one of which of claim 2 to 4 is it is characterised in that institute
State carrier body and load bearing unit thereon is made up of plastic material.
7. biochip carrier as claimed in claim 1 covers on one it is characterised in that further including,
On this, lid includes lid body on, is formed in the annular restraining portion of lid body lower surface on this；Always
Lead to this annular restraining portion with this on lid body perforation；On this lid body with lower surface with respect to this carrier
Body upper surface covers on this carrier body, this annular restraining portion stretch into this appearance carry space, surround and
It is limited to the periphery of this chip.
8. biochip carrier as claimed in claim 7 is it is characterised in that above-mentioned annular limits
Portion and perforation constitute a limiting unit, and lid body on can arrange 1～384 limiting unit,
This limiting unit is matrix arrangement.
9. biochip carrier as claimed in claim 8 it is characterised in that described upper lid body and
Limiting unit thereon is made of by integral molding techniques.
10. biochip carrier as claimed in claim 9 it is characterised in that described upper lid body and
Limiting unit thereon is made up of plastic material.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW104124605A TWI595223B (en) | 2015-07-29 | 2015-07-29 | Bio-chip carrier |
| TW104124605 | 2015-07-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106391160A true CN106391160A (en) | 2017-02-15 |
| CN106391160B CN106391160B (en) | 2018-07-31 |
Family
ID=58007783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510822276.2A Expired - Fee Related CN106391160B (en) | 2015-07-29 | 2015-11-24 | Biochip carrier |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN106391160B (en) |
| TW (1) | TWI595223B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020190839A1 (en) * | 1998-12-07 | 2002-12-19 | Aravind Padmanabhan | Integratable fluid flow and property microsensor assembly |
| TW201111782A (en) * | 2009-09-16 | 2011-04-01 | Nat Univ Tsing Hua | Anesthetic sensing optical microfluidic chip system |
| CN103341370A (en) * | 2008-10-28 | 2013-10-09 | 藤仓化成株式会社 | Liquid passage device and manufacturing method |
| TW201341004A (en) * | 2012-04-12 | 2013-10-16 | Univ Nat Cheng Kung | Device for liquid sample filtration |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2731423B2 (en) * | 1989-06-30 | 1998-03-25 | 日本商事株式会社 | Rotary cuvette |
| TWM488629U (en) * | 2014-01-22 | 2014-10-21 | Siward Crystal Technology Co Ltd | Sensing device |
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2015
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020190839A1 (en) * | 1998-12-07 | 2002-12-19 | Aravind Padmanabhan | Integratable fluid flow and property microsensor assembly |
| CN103341370A (en) * | 2008-10-28 | 2013-10-09 | 藤仓化成株式会社 | Liquid passage device and manufacturing method |
| TW201111782A (en) * | 2009-09-16 | 2011-04-01 | Nat Univ Tsing Hua | Anesthetic sensing optical microfluidic chip system |
| TW201341004A (en) * | 2012-04-12 | 2013-10-16 | Univ Nat Cheng Kung | Device for liquid sample filtration |
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| CN106391160B (en) | 2018-07-31 |
| TW201704731A (en) | 2017-02-01 |
| TWI595223B (en) | 2017-08-11 |
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