CN106390209A - Silk fibroin biological material compounded with epidermal growth factor and preparation method of silk fibroin biological material - Google Patents
Silk fibroin biological material compounded with epidermal growth factor and preparation method of silk fibroin biological material Download PDFInfo
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- CN106390209A CN106390209A CN201610799646.XA CN201610799646A CN106390209A CN 106390209 A CN106390209 A CN 106390209A CN 201610799646 A CN201610799646 A CN 201610799646A CN 106390209 A CN106390209 A CN 106390209A
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- epidermal growth
- growth factor
- fibroin
- fibroin albumen
- silk
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
Abstract
The invention provides a silk fibroin biological material compounded with the epidermal growth factor and a preparation method of the silk fibroin biological material. Silk fibroin is a natural high polymer material extracted from fibroin of silk, has good biocompatibility, is non-toxic to the body, has no sensitization and stimulation effects, and is partially biodegradable; the epidermal growth factor belongs to active substance in the human body, is peptide active substance composed of 53 amino acid residues, and can stimulate the tyrosine phosphorylation of receptors of the epidermal growth factors, so that the effect of repairing hyperplasia can be achieved, therefore, the silk fibroin biological scaffold prepared by adopting silk fibroin and the epidermal growth factor is degradable, and has the function of promoting the wound healing, and in addition, the operation is simple during the preparation of the silk fibroin biological scaffold.
Description
Technical field
The present invention relates to bio-medical technology field is and in particular to a kind of fibroin albumen of compound epidermal growth factor is biological
Material and preparation method thereof.
Background technology
Skin, as human organ, has holding moisture, breathes freely and prevent the functions such as bacteria attack.Dermal tissue is once damage
Wound then cannot regenerate completely, and clinically large-area deep dermis are damaged and generally repaiied using autologous, allosome or xenogenesis grafts
Multiple treatment, but these treatment meanss one side can cause new wound to donor.High score subclass used in tissue repair at present
Biomaterial includes synthesizing macromolecular material and natural macromolecular material, and synthesis macromolecular material is mainly the aliphatic such as polylactic acid
Polyester material, although it all substantially can meet the application of timbering material at the aspect such as mechanical property and degradability, in biology
There is many deficiencies in the aspects such as the compatibility, its catabolite easily produces harmful effect to organism.
In natural macromolecular material, silk fiber have the characteristics that source wide it is easy to commercial application.And fibroin is one
Plant the natural polymer protein extracting from silkworm silk, it includes 18 kinds of aminoacid, and amino acid classes are similar with human body skin, right
Human body has no toxic side effect, and biocompatibility is good.Through processing, fibroin albumen can be produced film forming, gel, support, manages, micro-
Ball, the materials application such as lyophilized powder is in various tissue engineerings.There is now University Of Suzhou's Li Ming loyalty et al. by fibroin albumen and to gather
Urethane material combines and is fabricated to the treatment that artificial skin is applied to deep burn wound.In existing patent, more is to utilize silk
Fibroin and the Material cladding such as sodium alginate, gelatin are fabricated to aerogel dressing or sponge dressing, fibroin albumen institute wherein
Work little.Epidermal growth factor (EGF) exchanges ganglion cell's growth, propagation and differentiation highly important effect.Existing
Numerous studies show, EGF can induce the propagation of various kinds of cell, particularly epidermis cell, endotheliocyte, can promote RNA, DNA
With the synthesis of protein, the synthesis of the macromole such as hyaluronic acid, elastic fiber albumen can be promoted, and then so that moisture content of skin is increased
Plus, elasticity increase.EGF is applied to burn and scald and the reparation of wound wound and healing has good curative effect：EGF can substantially add
The healing of fast traumatic wounds, and the neatly smooth of wound surface can be kept, advantageously reduce scar tissue and pigmentation.The present invention
First epidermal growth factor is combined with fibroin albumen compound bio support, produces one kind and can be applicable to cave type traumatic wounds
New pattern compress, widened the field of trauma care, be doctor and sufferer is provided convenience.
Content of the invention
The application provides a kind of silk having good biocompatibility and promoting the compound epidermal growth factor of wound healing
Fibroin biomaterial and preparation method thereof.
According in a first aspect, providing a kind of fibroin albumen biomaterial of compound epidermal growth factor in a kind of embodiment
Preparation method, comprises the steps：
Degumming：Silkworm silk is carried out degumming process and obtains boiled silk；
Dissolving dialysis：Boiled silk is placed in dissolving dialysis in saline solution and obtains silk fibroin protein solution；
Casting：Silk fibroin protein solution after dissolving dialysis is injected in mould, and silk fibroin protein solution will be marked with
Mould is placed in the silk fibroin bracket that lyophilization in freezer dryer obtains being dried porous；
Soak induction：Silk fibroin bracket is dipped in derivant, induction fibroin albumen structural transformation forms and do not dissolve in
The silk fibroin bracket of water；
Soak compound：Silk fibroin bracket after soaking is placed in the EGF/SF containing epidermal growth factor and fibroin albumen
In mixed liquor so that silk fibroin bracket surface uniform adhesion epidermal growth factor and the EGF/SF mixed liquor of fibroin albumen；
Lyophilization：Compound silk fibroin bracket after soaking is placed in lyophilization in freezer dryer and obtains composite table
The fibroin albumen biomaterial of skin growth factor.
Further, in degumming step, silkworm silk is placed in Na2CO3Heat degumming in solution, and dry prepared boiled silk.
Further, in dissolving dialysis step, saline solution is one of lithium bromide, magnesium nitrate, calcium chloride solution, and makes
Carry out dialysis treatment, prepared fibroin albumen weak solution one with bag filter.
Further, in dissolving dialysis step, fibroin albumen weak solution one need to be concentrated as fibroin albumen concentrated solution two, and
And concentration time is more than or equal to 48h.
Further, the time of freezer dryer freeze-drying process is 24～48h, and cryogenic temperature is -10 DEG C～-80
℃.
Further, soak in induction step, silk fibroin bracket is more than 1 with the mass ratio of derivant:20, soak time
For 0.5～24h.
Further, EGF/SF mixed liquor is 50 μ g/mL by concentration epidermal growth factor and fibroin albumen weak solution one
Mix.
Further, the mass ratio of EGF/SF mixed liquor Concentrations of Epidermal Growth Factor and fibroin albumen weak solution one is 1:20～
200, and mix under conditions of 37 DEG C.
Further, during immersion is compound, EGF/SF mixed liquor is 1 with the mass ratio of silk fibroin bracket:1.
According to second aspect, in a kind of embodiment, provide a kind of fibroin albumen biomaterial of compound epidermal growth factor,
It is characterized in that, comprise fibroin albumen and epidermal growth factor, and given birth to by the fibroin albumen weighing above-mentioned compound epidermal growth factor
The preparation method of thing material is prepared from.
Fibroin albumen biomaterial of compound epidermal growth factor according to above-described embodiment and preparation method thereof, due to silk
Fibroin is a kind of natural macromolecular material extracting from natural silk, has good biocompatibility, nontoxic to body
Property, no sensitization and stimulation, and partially biodegradable, epidermal growth factor (EGF) is a kind of active substance in human body,
The peptides active substance being made up of 53 amino acid residues, can stimulate the tyrosine phosphorylation of EGFR,
Reach the effect repairing hypertrophy so that degradable and promotion are had by the biomaterial that fibroin albumen and epidermal growth factor are made
The effect of wound healing, and its preparation manipulation is simple.
Brief description
Fig. 1 is a kind of flow process of the preparation method of fibroin albumen biomaterial of compound epidermal growth factor in embodiment
Figure.
Specific embodiment
Combine accompanying drawing below by specific embodiment the present invention is described in further detail.
Embodiment one：
Present embodiments provide a kind of preparation method of the fibroin albumen biomaterial of compound epidermal growth factor, as schemed institute
Show, comprise the steps：
S101：Degumming；
30g silkworm raw silk is put into 12L and contains Na2CO3(2.12g/L) stir in boiling water and boil 30mins, during cooking
Degumming rate is made to improve with the continual stirring of Glass rod.Wait cook good after deionized water to rub well-done degumed silk residual to remove
The sericin staying.Fibroin fiber is placed in aeration-drying in fume hood.
S102：Dissolving dialysis；
Weigh above-mentioned prepared boiled silk 15g to put in lithium bromide (LiBr) solution that 100mL concentration is 9.3mol/L
Carry out dissolution process, dissolve 4 hours under conditions of 60 DEG C during dissolution process.Per hour with Glass rod solution is stirred with
Guarantee silk fiber dissolving completely.
Again dialysis treatment is carried out to the lithium-bromide solution dissolved with boiled silk, during dialysis by molecular cut off be 3500 saturating
Analysis bag is immersed in deionized water dialyses 3 days, and period every two hours respectively changes a deionized water, to remove the lithium bromide in solution
(LiBr) the pure fibroin albumen weak solution one, obtaining, the concentration of this silk fibroin protein solution is 6%, and concentration is relatively low, in order to carry
The concentration of high fibroin albumen, instant freeze dried, concentration need to be carried out, for example, concentrated, warp using Polyethylene Glycol (PEG 400)
Cross and be concentrated to give the fibroin albumen concentrated solution two that concentration is 30%, wherein concentration time is more than or equal to 48h, is preferably 72h.
S103：Casting；
The silk fibroin protein solution that concentration is 30% is injected in the good mould of pre-production, fibroin albumen shape in a mold
Become supporting structure set in advance, again mould is placed in liquid nitrogen freezing dehydrator after having injected and carries out lyophilization, at freezing
The temperature of reason is -10 DEG C～-80 DEG C, preferably -30 DEG C, and the cryodesiccated time is 24～48h, preferably 48h, by freezing
The silk fibroin bracket of dry porous is obtained after drying.
S104：Soak induction；
The silk fibroin bracket being dried is dipped in derivant, derivant is preferably methanol solution, silk fibroin bracket
It is more than 1 with the mass ratio of methanol:20, soak time is 0.5～24h.Methanol can induce fibroin albumen structural transformation, and structure turns
Silk fibroin bracket after change is water insoluble.Silk fibroin bracket after induction changes is placed in aeration-drying in fume hood, makes silk
The methanol volatilization of residual on fibroin support.In other embodiments, derivant also can be selected for ethanol, or other have induction
The derivant of effect.
S105：Soak compound；
By concentration be 50 μ g/mL epidermal growth factor (EGF) and fibroin albumen (SF) weak solution one is pressed under the conditions of 37 DEG C
It is 1 according to mass ratio:20～200 mix homogeneously, make SF/EGF mixed liquor.Fibroin albumen water-fast described in S105 is frozen
Heavenly Stems and Earthly Branches frame is 1 in mass ratio:1 puts in SF/EGF mixed liquor, so that silk fibroin bracket surface is uniformly adhered to containing SF/
EGF mixed liquor.
S106：Lyophilization.
Finally the silk fibroin bracket after soaking and being combined is put in freezer dryer again after liquid nitrogen freezing
Lyophilization, obtains the fibroin albumen biological support of the water-fast porous of extexine water soluble internal carrier structure.
The fibroin albumen biomaterial of the compound epidermal growth factor of preparation method preparation that this example provides, can be used as hole
The packing material of type wound upper ledge.
Because fibroin albumen is a kind of natural macromolecular material extracting from natural silk, there is good bio-compatible
Property, to body avirulence, no sensitization and stimulation, and partially biodegradable, its catabolite itself is not only to tissue no
Toxic and side effects, also nutritious to such as skin, periodontal tissue etc. the effect with reparation.Numerous studies prove, fibroin albumen is basis set to be knitted
Engineering rack can be applied to bone, skin, blood vessel, nerve, liver, cartilage, the regeneration of ligament Various Tissues.
Epidermal growth factor (EGF) is a kind of active substance in human body, is lived by the peptides that 53 amino acid residues form
Property material, molecular weight 6201Da, three pairs of disulfide bond that intramolecular is made up of 6 cysteine, form 3 molecule inner ring types knot
Structure, receptorbinding region necessary to composition biological activity.It can stimulate the tyrosine phosphatase of EGFR
Change, reach the effect repairing hypertrophy.Excellent curative effect is had to injured, impaired epidermis skin.Epidermal growth factor EGF can also
Hemostasis, and there is acceleration skin and mucosa wound healing, anti-inflammatory analgesic, strengthen the vigor of epidermis cell, can be various with human body
Enzyme forms good coordinating effect.
So that degradable being had by the biomaterial that fibroin albumen and epidermal growth factor are made and promoting wound healing
Effect, and its preparation manipulation is simple.
Embodiment two：
This example provides a kind of fibroin albumen biomaterial of compound epidermal growth factor, the silk of compound epidermal growth factor
Fibroin biomaterial fibroin albumen and epidermal growth factor, are prepared from by the preparation method of above-described embodiment one.
Effect degradable being had by the biomaterial that fibroin albumen and epidermal growth factor are made and promoting wound healing,
Therefore this material can be used as filling inside the type wound of hole, outside coats wound dressing again, promotes the healing of wound.
Use above specific case is illustrated to the present invention, is only intended to help and understands the present invention, not in order to limit
The present invention processed.For those skilled in the art, according to the thought of the present invention, can also make some simple
Deduce, deform or replace.
Claims (9)
1. a kind of fibroin albumen biomaterial of compound epidermal growth factor, its preparation method, comprise the steps：
Degumming：Silkworm silk is carried out degumming process and obtains boiled silk；
Dissolving dialysis：Boiled silk is placed in dissolving dialysis in saline solution and obtains silk fibroin protein solution；
Casting：Silk fibroin protein solution after dissolving dialysis is injected in mould, and the mould of silk fibroin protein solution will be marked with
It is placed in the silk fibroin bracket that lyophilization in freezer dryer obtains being dried porous；
Soak induction：Silk fibroin bracket is dipped in derivant, induction fibroin albumen structural transformation forms water-fast
Silk fibroin bracket；
Soak compound：Silk fibroin bracket after soaking is placed in the mixing of the EGF/SF containing epidermal growth factor and fibroin albumen
In liquid so that silk fibroin bracket surface uniform adhesion epidermal growth factor and the EGF/SF mixed liquor of fibroin albumen；
Lyophilization：By soak compound after silk fibroin bracket be placed in lyophilization in freezer dryer and obtain compound epidermis
The fibroin albumen biomaterial of somatomedin.
2. it is combined the preparation method of the fibroin albumen biomaterial of epidermal growth factor as claimed in claim 1, its feature exists
In, in described dissolving dialysis step, described saline solution is one of lithium bromide, magnesium nitrate, calcium chloride solution, and using dialysis
Bag carries out dialysis treatment, prepared fibroin albumen weak solution one.
3. it is combined the preparation method of the fibroin albumen biomaterial of epidermal growth factor as claimed in claim 1, its feature exists
In described dissolving is dialysed in step, fibroin albumen weak solution one need to be carried out concentration, and concentration time is more than or equal to
48h, obtains fibroin albumen concentrated solution two.
4. it is combined the preparation method of the fibroin albumen biomaterial of epidermal growth factor as claimed in claim 1, its feature exists
In the time of described freezer dryer freeze-drying process is 24～48h, and cryogenic temperature is -10 DEG C～-80 DEG C.
5. it is combined the preparation method of the fibroin albumen biomaterial of epidermal growth factor as claimed in claim 1, its feature exists
In, in described immersion induction step, described derivant is one of methanol, ethanol, the matter of silk fibroin bracket and derivant
Amount ratio is more than 1:20, soak time is 0.5～24h.
6. it is combined the preparation method of the fibroin albumen biomaterial of epidermal growth factor as claimed in claim 1, its feature exists
In by concentration, the epidermal growth factor being 50 μ g/mL and fibroin albumen weak solution one mix described EGF/SF mixed liquor.
7. it is combined the preparation method of the fibroin albumen biomaterial of epidermal growth factor as claimed in claim 1, its feature exists
In the mass ratio of described EGF/SF mixed liquor Concentrations of Epidermal Growth Factor and fibroin albumen weak solution one is 1:20～200, and
Mix under conditions of 37 DEG C.
8. it is combined the preparation method of the fibroin albumen biomaterial of epidermal growth factor as claimed in claim 1, its feature exists
In, during described immersion is compound, described EGF/SF mixed liquor is 1 with the mass ratio of silk fibroin bracket:1.
9. a kind of fibroin albumen biomaterial of compound epidermal growth factor is it is characterised in that comprise fibroin albumen and epidermis life
The long factor, and the preparation of the fibroin albumen biomaterial by the compound epidermal growth factor any one of claim 1 to 8
Method is prepared from.
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| CN201610799646.XA CN106390209A (en) | 2016-08-31 | 2016-08-31 | Silk fibroin biological material compounded with epidermal growth factor and preparation method of silk fibroin biological material |
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Cited By (8)
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| CN110141657A (en) * | 2019-05-17 | 2019-08-20 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | A kind of epidermal growth factor liposome and preparation method thereof |
| CN112107737A (en) * | 2020-07-30 | 2020-12-22 | 九魁(苏州)医疗科技有限公司 | Medical catheter with medicine slow release function |
| CN112852876A (en) * | 2021-03-04 | 2021-05-28 | 西南大学 | Silkworm silk gland recombinant expression vector for expressing human epidermal growth factor and preparation method and application thereof |
| WO2022028106A1 (en) * | 2020-08-06 | 2022-02-10 | 苏州大学 | Silk fibroin sericin composite membrane and preparation method therefor |
| WO2022028105A1 (en) * | 2020-08-06 | 2022-02-10 | 苏州大学 | Bionic composite bone scaffold and preparation method therefor |
| CN114848907A (en) * | 2022-05-12 | 2022-08-05 | 苏州苏豪生物材料科技有限公司 | Growth factor-loaded silk fibroin |
| CN115137883A (en) * | 2022-08-03 | 2022-10-04 | 尧舜泽生物医药(南京)有限公司 | Bionic composite mineralized scaffold and preparation method thereof |
| CN115252897A (en) * | 2022-08-10 | 2022-11-01 | 北京航空航天大学 | Cartilage tissue repair scaffold capable of sequentially and continuously releasing polypeptides and factors and preparation method thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110141657A (en) * | 2019-05-17 | 2019-08-20 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | A kind of epidermal growth factor liposome and preparation method thereof |
| CN110141657B (en) * | 2019-05-17 | 2023-01-17 | 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 | Epidermal growth factor liposome and preparation method thereof |
| CN112107737A (en) * | 2020-07-30 | 2020-12-22 | 九魁(苏州)医疗科技有限公司 | Medical catheter with medicine slow release function |
| WO2022028106A1 (en) * | 2020-08-06 | 2022-02-10 | 苏州大学 | Silk fibroin sericin composite membrane and preparation method therefor |
| WO2022028105A1 (en) * | 2020-08-06 | 2022-02-10 | 苏州大学 | Bionic composite bone scaffold and preparation method therefor |
| CN112852876A (en) * | 2021-03-04 | 2021-05-28 | 西南大学 | Silkworm silk gland recombinant expression vector for expressing human epidermal growth factor and preparation method and application thereof |
| CN112852876B (en) * | 2021-03-04 | 2022-11-29 | 西南大学 | Silkworm silk gland recombinant expression vector for expressing human epidermal growth factor and preparation method and application thereof |
| CN114848907A (en) * | 2022-05-12 | 2022-08-05 | 苏州苏豪生物材料科技有限公司 | Growth factor-loaded silk fibroin |
| CN115137883A (en) * | 2022-08-03 | 2022-10-04 | 尧舜泽生物医药(南京)有限公司 | Bionic composite mineralized scaffold and preparation method thereof |
| CN115137883B (en) * | 2022-08-03 | 2023-12-29 | 尧舜泽生物医药(南京)有限公司 | Bionic composite mineralization bracket and preparation method thereof |
| CN115252897A (en) * | 2022-08-10 | 2022-11-01 | 北京航空航天大学 | Cartilage tissue repair scaffold capable of sequentially and continuously releasing polypeptides and factors and preparation method thereof |
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Application publication date: 20170215 |