CN106370874A - Ultramicro nucleic acid protein analyzer automatic sample feeding detection device - Google Patents
Ultramicro nucleic acid protein analyzer automatic sample feeding detection device Download PDFInfo
- Publication number
- CN106370874A CN106370874A CN201610750313.8A CN201610750313A CN106370874A CN 106370874 A CN106370874 A CN 106370874A CN 201610750313 A CN201610750313 A CN 201610750313A CN 106370874 A CN106370874 A CN 106370874A
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- China
- Prior art keywords
- fixed
- detection probe
- mandrel
- decelerator
- sample
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
Abstract
The invention relates to an ultramicro nucleic acid protein analyzer automatic sample feeding detection device. Efficiency and accuracy of detection and analysis of nucleic acid protein are greatly improved. According to the scheme, the device comprises a probe control mechanism, a cleaning mechanism, a sample dispensing mechanism and a sample sending mechanism. The probe control mechanism is controlled by a stepping motor to achieve vertical swinging of an upper detection probe. The cleaning mechanism is controlled by a stepping motor to drive a fire mandrel to rotate, cleaning paper on a second mandrel is wound around the first mandrel, and then the upper detection probe and a lower detection probe are cleaned. The sample dispensing mechanism injects samples in a sample gun to the lower detection probe, and the sample sending mechanism drives the cleaning mechanism and the sample dispensing mechanism to horizontally move. The volume control of the detection and analysis samples is more precise, an automatic control device is used, automatic sample feeding detection is achieved, efficiency and accuracy of detection and analysis of nucleic acid protein are obviously improved, inspection operation is simple, and unnecessary labor of relevant researcher is reduced.
Description
Technical field
The present invention relates to medical laboratory instrument, especially relate to a kind of ultramicron nucleic acid-protein analyser and automatically go up sample inspection
Survey device.
Background technology
On existing ultramicron nucleic acid-protein analyser, in pattern detection operating process, first have to detection probe
Cleared up, then some circulate operations such as sample detection analysis, cleaning, loading, analysis, cleaning again.Detection person is at this
In operating process, computer also to be operated, analysis result is arranged, the loaded down with trivial details and easy error of whole process.
Content of the invention
For above-mentioned situation, for overcoming the defect of prior art, the purpose of the present invention is exactly to provide a kind of ultramicron nucleic acid
Protein analyzer goes up sample testing apparatus automatically, on the basis of existing ultramicron nucleic acid-protein analyser, increases present invention dress
Put so that the efficiency of the detection and analysis of nucleic acid-protein and accuracy rate greatly improve so that related scientific research personnel are in scientific research
More handy.
Its solution is, including probe operating mechanism, wiper mechanism, point sample mechanism, sample feeding mechanism；
Described probe operating mechanism includes being fixedly mounted on the first bearing of nucleic acid-protein analyser upper surface, solid on the first bearing
It is connected with the first decelerator, the first reducer input shaft is coaxially fixed with the first motor being fixed on the first bearing, first
The vertical output shaft of decelerator is fixed with the driving fork being transversely to the machine direction output shaft axis, and one end of described driving fork is connected
On the vertical output shaft of the first decelerator, the free end of fork is driven to be connected with rod ends, the oriented one end of rod ends band
The cavity that face is opened, is fixed with the spring stretching along side pole joint axis in cavity, one end of spring is connected in the bottom of cavity,
The free end of spring is connected in and drives the free splaying of fork to extend into rod ends on the end face driving fork free end
In cavity, the head of rod ends is hinged with detection probe depression bar, hinged with rod ends in the middle part of detection probe depression bar, and detection is visited
Positioned at the left side of the first reducer output shaft, detection probe depression bar is freely on lower hinge first bearing of head depression bar and hinged place
End is fixed with detection probe；
A kind of described ultramicron nucleic acid-protein analyser is automatically gone up sample testing apparatus and is also included being fixed on nucleic acid-protein analyser
The guide-rail plate of upper surface, guide-rail plate is fixed with horizontal first guide rail and is located at horizontal with the first guide rail parallel on the left of guide-rail plate
Second guide rail；
Described wiper mechanism includes the second bearing being connected with the first slide, and the second bearing is fixed with the second decelerator,
The input shaft of the second decelerator is coaxially fixed with the second motor being fixed on the second bearing, and the longitudinal direction of the second decelerator is defeated
Shaft is coaxially fixed with axis and guide rail parallel and is located at the first mandrel of the first guide upper, and the second bearing is also rotatably connected to
The second mandrel being longitudinally spaced-apart with the first mandrel, the axis of the second mandrel is with the first mandrel diameter parallel and height phase
Being fixed with volume with, the second mandrel has the clean paper roll cleaning paper, have between the first mandrel and the second mandrel position fixing and
Can with the lower detection probe that contact of upper detection probe under the effect of detection probe depression bar, between the first mandrel and the second mandrel under
Above detection probe, there is the pressure of two be rotatably connected on the second bearing and axis and the first mandrel diameter parallel longitudinal both sides
Roller, cleaning paper be adjacent to bypass two pressure rollers bottom volume be enclosed within the first mandrel, cleaning the following of paper can be close to lower detection probe
And detection probe can be close to above, described second bearing is fixedly connected with the pull bar parallel with the first guide rail direction；
Described point sample mechanism include on the left of wiper mechanism and lower detection probe and be connected pull bar and with the second slide
The support connecting, support is fixedly connected with the point sample rifle tilting towards bottom right, support is fixed with the 3rd decelerator, the 3rd subtracts
The input shaft of fast device is coaxially fixed with the 3rd motor being fixed on support, and the output shaft of the 3rd decelerator is coaxially fixed with
First nut of axis inclined upward to the left, the first nut screw is connected with axis and point sample gun axis line is parallel to be robbed positioned at point sample
First screw mandrel of lower left, one end of the first screw mandrel screws in the first nut, and its free end is fixed with vertical first leading screw axis
Pressing plate, pressing plate is also connected in its bottom-right point sample rifle piston by hand, and the head of described point sample rifle is towards lower right and energy
In detection probe under being enough located at the top of lower detection probe and injecting sample under pressing plate function, described support bottom is fixed with
Diameter parallel is in the second nut of the second guide rail；
Described sample feeding mechanism includes the 4th decelerator being connected on guide-rail plate, and the input shaft of the 4th decelerator is coaxially fixed with admittedly
It is scheduled on the 4th motor on final drive casing, coaxially connected second leading screw of horizontal output shaft of the 4th decelerator, second
Thick stick is connected with the second nut screw.
The invention enables controlling more accurately to the amount of check analyses sample, using automatic control device, realize automatically
Pattern detection in change so that nucleic acid-protein detection and analysis efficiency and accuracy rate significantly improves so that checks is simple to operate, subtract
The unnecessary work of few related scientific research personnel, has very high application and popularization value.
Brief description
Fig. 1 is front view schematic diagram of the present invention.
Fig. 2 is top view schematic diagram of the present invention.
Fig. 3 is detection probe operating mechanism schematic diagram of the present invention.
Fig. 4 is wiper mechanism front view schematic diagram of the present invention.
Fig. 5 is wiper mechanism left view schematic diagram of the present invention.
Fig. 6 is point sample mechanism schematic diagram of the present invention.
Fig. 7 is deposit structural scheme of mechanism of the present invention.
Specific embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Be given by Fig. 1 to Fig. 7, a kind of ultramicron nucleic acid-protein analyser automatically go up sample testing apparatus it is characterised in that
Including probe operating mechanism 1, wiper mechanism 2, point sample mechanism 3, sample feeding mechanism 4；
Described probe operating mechanism includes the first bearing 9 being fixedly mounted on nucleic acid-protein analyser 5 upper surface, the first bearing 9
On be fixed with the first decelerator 8, the first decelerator 8 input shaft is coaxially fixed with the first motor being fixed on the first bearing
7, the vertical output shaft of the first decelerator 8 is fixed with the driving fork 10 being transversely to the machine direction output shaft axis, described driving fork
10 one end is connected on the vertical output shaft of the first decelerator 8, drives the free end of fork 10 to be connected with rod ends 12, bar
The cavity that end connector 12 is opened with oriented end face, is fixed with the spring 11 stretching along side pole joint 12 axis, spring in cavity
11 one end is connected in the bottom of cavity, and the free end of spring 11 is connected on the end face driving fork 10 free end, drives pendulum
The free splaying of bar 10 extend in the cavity of rod ends 12, and the head of rod ends 12 is hinged with detection probe depression bar 6,
Detection probe depression bar 6 middle part, lower hinge first bearing 9 of detection probe depression bar 6 on and hinged place hinged with rod ends 12
Positioned at the left side of the first reducer output shaft, detection probe depression bar 6 free end is fixed with detection probe 13；
A kind of described ultramicron nucleic acid-protein analyser is automatically gone up sample testing apparatus and is also included being fixed on nucleic acid-protein analyser
The guide-rail plate 35 of upper surface, guide-rail plate 35 is fixed with horizontal first guide rail 20 and is located at flat with the first guide rail 20 on the left of guide-rail plate
Horizontal second guide rail 30 of row；
Described wiper mechanism includes the second bearing 17 being slidably connected with the first guide rail 20, the second bearing 17 is fixed with second and subtracts
Fast device 16, the input shaft of the second decelerator 16 is coaxially fixed with the second motor 15 being fixed on the second bearing, and second subtracts
The vertical output shaft of fast device 16 is coaxially fixed with axis and guide rail parallel and is located at the first mandrel 18 of the first guide upper, and second
Bearing 17 is also rotatably connected to the second mandrel 23 of parallel with the first mandrel 18 and longitudinally spaced setting, the second mandrel 23 fixed cover
There is volume to have the clean paper roll 24 cleaning paper, have that position is fixed between the first mandrel 18 and the second mandrel 23 and can visit with detection
The lower detection probe 14 of upper detection probe 13 contact under head depression bar effect, lower detection between the first mandrel 18 and the second mandrel 23
There are two be rotatably connected on the second bearing 17 and axis and the first mandrel 18 diameter parallel in the longitudinal both sides in probe 14 top
Pressure roller 20, cleaning paper be adjacent to bypass two pressure rollers 20 bottom volume be enclosed within the first mandrel 18, cleaning paper following can be close under
Detection probe 14 and can be close to detection probe 13 above, described second bearing 17 is fixedly connected with and the first guide rail 20 direction
Parallel pull bar 21；
Described point sample mechanism include positioned at wiper mechanism 2 and lower detection probe 14 left side and connected pull bar 21 and with the second guide rail
The support 22 that 30 are slidably connected, support is fixedly connected with the point sample rifle tilting towards bottom right, support 22 is fixed with the 3rd and subtracts
Fast device 28, the input shaft of the 3rd decelerator 28 is coaxially fixed with the 3rd motor 29 being fixed on support 22, the 3rd deceleration
The output shaft of device 28 is coaxially fixed with the first nut of axis inclined upward to the left, and the first nut screw is connected with axis and point sample
This rifle 25 diameter parallel robs the first screw mandrel 27 of lower left positioned at point sample, and one end of the first screw mandrel 27 screws in the first nut, its
Free end is fixed with the pressing plate 26 of vertical first leading screw axis, and pressing plate 26 is also connected in its bottom-right point sample rifle 25 piston by handss
On, the head of described point sample rifle 25 towards lower right and can be located at the top of lower detection probe and by sample under pressing plate 25 effect
In the lower detection probe 14 of this injection, described support 22 bottom is fixed with diameter parallel in the second nut 34 of the second guide rail 30；
Described sample feeding mechanism includes the 4th decelerator 32 being connected on guide-rail plate 35, and the input shaft of the 4th decelerator 32 is coaxially solid
It is connected with the 4th motor 33 being fixed on final drive casing, coaxially connected second of the horizontal output shaft of the 4th decelerator 32
Thick stick 31, the second leading screw 31 is connected with the second nut screw 34.
The present invention can be used in INSTRUMENT MODEL entitled thermo scientific:nanodrop 1000
On the ultramicron nucleic acid-protein analyser of spectrophotometer
When the present invention uses, four motors, in the control of Single Chip Microcomputer (SCM) program, realize corresponding action, the time of detection,
Every all can controlling.Sample sample is placed in a sample rifle 25, the 4th motor 33 drives the second screw mandrel 31 to revolve
Turn and nut 34 and support 22 are moved along the downward detection probe direction of the second guide rail 30, and then by being connected on support 22
Pull bar 21 drive wiper mechanism 2 to same direction mobile so that cleaning paper is completely covered and entirely descends detection probe 14.Then
4th motor 33 quits work, and the first motor 7 starts so that detection probe depression bar 6 turns to detection probe 13
Contact cleaning paper after the first motor 7 quit work, the second motor 15 works, drive the first mandrel 18 rotate so that
Cleaning paper roll on the first mandrel 18, then will be visited by the cleaning paper through upper detection probe 13 and lower detection probe 14 by upper detection
13 and lower detection probe 14 clean up.After cleaning terminates, the second motor 15 quits work, the first motor work
Starting 7 makes detection probe depression bar 6 be raised up to certain altitude, and then the first motor 7 quits work, the 4th motor 33
Work so that sample presentation mechanism 3 and wiper mechanism 2 continue to move right along guide rail direction, when a head for sample rifle 25 is located at
During lower detection probe 14 top, and now wiper mechanism is located at the right side of lower detection probe 14, and the 4th motor 33 stops work
Make, the 3rd stepping 29 electric motor starting, the 3rd motor 29 drives the first nut rotation so that the first screw mandrel 27 and pressing plate 26 edge
Lower right moves, and puts sample rifle 25 point sample in lower detection probe 14.After point sample terminates, the 3rd motor 29 stops work
Make, the 4th motor 33 works so that sample presentation mechanism 3 and wiper mechanism 2 are moved to the left certain distance, now wiper mechanism
Cleaning paper be still located on the right side of lower detection probe 14.4th motor 33 quits work, and the first motor 7 works, and makes
Obtain detection probe depression bar 6 to depress, and then so that detection probe 13 is contacted with lower detection probe 14, to sample pattern detection.Detection
After end, the first motor 7 works so that detection probe depression bar 6 lifts, and then then the first motor 7 quits work
4th motor work 33 makes sample presentation mechanism 3 and wiper mechanism 2 be moved to the left and clean detection probe 14 under paper covers, and the 4th
Motor 33 quits work, and the first motor work 7 makes detection probe depression bar 6 pressure contact cleaning paper, and then first
Motor 7 quits work, and the second motor 15 works, and upper detection probe 13 and lower detection probe 14 is carried out, clearly
After washing end, the second motor 15 quits work, and after detection is visited, depression bar 6 lifts, and sample presentation mechanism 3 and wiper mechanism 2 are to moving to left
Move to original position, complete a cycle of operation, when checking for second, repeat said process, fully achieve sample inspection in automatization
Survey.Because driving fork and rod ends to be to achieve elastic connection by spring, detection process can be protected detection
Probe and lower detection probe are not subject to crushing, and point sample mechanism realization automatic words control ensure that the consumption of sample sample accurately, makes
Obtain test result more reliable
Embodiment described above is not defined to the scope of the present invention, in the premise without departing from design concept of the present invention
Under, various modifications and improvement that technical staff described in this area makes to technical scheme, all should include the present invention's
In the protection domain that claims determine.
Claims (1)
1. a kind of ultramicron nucleic acid-protein analyser goes up sample testing apparatus automatically it is characterised in that including probe operating mechanism
(1), wiper mechanism (2), point sample mechanism (3), sample feeding mechanism (4)；
The first bearing (9) that described operating mechanism of popping one's head in includes being fixedly mounted on nucleic acid-protein analyser (5) upper surface, first
It is fixed with the first decelerator (8), the first decelerator (8) input shaft is coaxially fixed with the being fixed on the first bearing on seat (9)
One motor (7), the vertical output shaft of the first decelerator (8) is fixed with the driving fork being transversely to the machine direction output shaft axis
(10), described one end driving fork (10) is connected on the vertical output shaft of the first decelerator (8), drives fork (10) oneself
Rod ends (12), the cavity that rod ends (12) are opened with oriented end face are connected with by end, are fixed with along side pole in cavity
The spring (11) that joint (12) axis stretches, one end of spring (11) is connected in the bottom of cavity, and the free end of spring (11) is solid
The sky driving the free splaying of fork (10) to extend into rod ends (12) is connected on the end face driving fork (10) free end
Intracavity, the head of rod ends (12) is hinged with detection probe depression bar (6), in the middle part of detection probe depression bar (6) and rod ends
(12) hinged, the lower hinge first bearing (9) of detection probe depression bar (6) is upper and hinged place is located at the first reducer output shaft
Left side, detection probe depression bar 6 free end is fixed with detection probe (13)；
A kind of described ultramicron nucleic acid-protein analyser is automatically gone up sample testing apparatus and is also included being fixed on nucleic acid-protein analyser
The guide-rail plate (35) of upper surface, guide-rail plate (35) is fixed with horizontal first guide rail (20) and leads with first on the left of guide-rail plate
Parallel horizontal second guide rail (30) of rail (20)；
Described wiper mechanism includes the second bearing (17) being slidably connected with the first guide rail (20), and the second bearing is fixed with (17)
Second decelerator (16), the input shaft of the second decelerator (16) is coaxially fixed with the second motor being fixed on the second bearing
(15), the vertical output shaft of the second decelerator (16) is coaxially fixed with axis and guide rail parallel and is located at the of the first guide upper
One mandrel (18), the second bearing (17) is also rotatably connected to the second mandrel of parallel with the first mandrel (18) and longitudinally spaced setting
(23), the second mandrel (23) is fixed with volume clean paper roll (24), the first mandrel (18) and the second mandrel (23) cleaning paper
Between have the lower detection probe that position is fixing and can contact with the upper detection probe (13) under the effect of detection probe depression bar
(14), above lower detection probe (14) between the first mandrel (18) and the second mandrel (23), longitudinal both sides have two to be rotatably connected on
On second bearing (17) and axis and the first mandrel (18) diameter parallel pressure roller (20), cleaning paper is adjacent to and bypasses two pressure rollers
(20) bottom volume is enclosed within the first mandrel (18), the following lower detection probe (14) and above can be tight can be close to of cleaning paper
Stick detection probe (13), described second bearing (17) is fixedly connected with the pull bar (21) parallel with the first guide rail (20) direction；
Described point sample mechanism includes on the left of wiper mechanism (2) and lower detection probe (14) and is connected pull bar (21) and with the
The support (22) that two guide rails (30) are slidably connected, support is fixedly connected with the point sample rifle tilting towards bottom right, on support (22)
It is fixed with the 3rd decelerator (28), the input shaft of the 3rd decelerator (28) is coaxially fixed with the 3rd step being fixed on support (22)
Stepper motor (29), the output shaft of the 3rd decelerator (28) is coaxially fixed with the first nut of axis inclined upward to the left, the first spiral shell
Female spiral is connected with axis and point sample rifle (25) diameter parallel robs first screw mandrel (27) of lower left, the first screw mandrel positioned at point sample
(27) one end screws in the first nut, and its free end is fixed with the pressing plate (26) of vertical first leading screw axis, and pressing plate (26) is also
It is connected in its bottom-right point sample rifle (25) piston to press on hand, the head of described point sample rifle 25 towards lower right and can be located at down
The top of detection probe under injecting sample under pressing plate (25) effect in detection probe (14), described support (22) bottom is solid
It is connected with diameter parallel in second nut (34) of the second guide rail (30)；
Described sample feeding mechanism includes the 4th decelerator (32) being connected on guide-rail plate (35), the input shaft of the 4th decelerator (32)
Coaxially it is fixed with the 4th motor (33) being fixed on final drive casing, the horizontal output shaft of the 4th decelerator (32) is coaxial
Connect the second leading screw (31), the second leading screw (31) is connected with the second nut screw (34).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610750313.8A CN106370874B (en) | 2016-08-27 | 2016-08-27 | A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610750313.8A CN106370874B (en) | 2016-08-27 | 2016-08-27 | A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106370874A true CN106370874A (en) | 2017-02-01 |
| CN106370874B CN106370874B (en) | 2017-11-17 |
Family
ID=57903082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610750313.8A Expired - Fee Related CN106370874B (en) | 2016-08-27 | 2016-08-27 | A kind of ultramicron nucleic acid-protein analyzer goes up sample testing apparatus automatically |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106370874B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1878496A1 (en) * | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Apparatus for performing nucleic acid analysis |
| CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
| CN203929772U (en) * | 2014-06-27 | 2014-11-05 | 珠海科域生物工程有限公司 | A kind of sampling point sample cleaning device |
-
2016
- 2016-08-27 CN CN201610750313.8A patent/CN106370874B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1878496A1 (en) * | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Apparatus for performing nucleic acid analysis |
| CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
| CN203929772U (en) * | 2014-06-27 | 2014-11-05 | 珠海科域生物工程有限公司 | A kind of sampling point sample cleaning device |
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| Publication number | Publication date |
|---|---|
| CN106370874B (en) | 2017-11-17 |
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