CN106360216A - Production method for red-fleshed kiwifruit ferment liquid - Google Patents

Production method for red-fleshed kiwifruit ferment liquid Download PDF

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Publication number
CN106360216A
CN106360216A CN201610746845.4A CN201610746845A CN106360216A CN 106360216 A CN106360216 A CN 106360216A CN 201610746845 A CN201610746845 A CN 201610746845A CN 106360216 A CN106360216 A CN 106360216A
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China
Prior art keywords
red
production method
enzyme liquid
red kiwifruit
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CN201610746845.4A
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Chinese (zh)
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王贵
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Sichuan Modern Agricultural Ltd By Share Ltd
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Sichuan Modern Agricultural Ltd By Share Ltd
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Priority to CN201610746845.4A priority Critical patent/CN106360216A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention discloses a production method for red-fleshed kiwifruit ferment liquid. The production method comprises the following processes: pectinase, cellulase and yeasts are added into squeezed and residual fruit flesh to conduct a primary fermentation, then sucrose and acetic acid bacteria are added to conduct a strain culture, and finally the fruit juice is subjected to a low temperature fermentation. The red-fleshed kiwifruits are fermented under the low temperature to prepare the ferment liquid, so that the production method increases the nutrient utilization rates of the red-fleshed kiwifruits, reduces the damages of other nutrients in the red-fleshed kiwifruits, and especially reduces the damage of Vc. The Vc content of the prepared ferment liquid is reduced to be 10% or less compared with the Vc content of the fresh kiwi fruits.

Description

A kind of production method of red kiwifruit enzyme liquid
Technical field
The present invention relates to the technical field of enzyme liquid preparation, particularly to the technical field of Fructus actinidiae chinensiss enzyme liquid preparation.
Background technology
Fructus actinidiae chinensiss also known as " kiwi fruit ", " Solanum muricatum " etc., the fertile soft succulence of fruit, sour-sweet pleasant, contain organic in fruit simultaneously Acid, vitamin enrich, and particularly the more general fruit of vc content exceeds several times to tens times, high in nutritive value.
Fructus actinidiae chinensiss sarcocarp is in the majority with green, and yellow is taken second place, and redness is the most rare, is the exclusive kind of China, and such as Sichuan is " red Sun ", " red U.S. " etc..By red kiwifruit each kind moisture is shown to the research of moisture, soluble solid content etc. The relatively green heart or gold zone Fructus actinidiae chinensiss are few, soluble solid content relative to highest, illustrate that the nutrition containing in red kiwifruit becomes Divide at most, further total acid content, ph analysis, content of reducing sugar, vc content, flavones content, dietary fiber content etc. detect Research finds that red kiwifruit acid content is low, and sugar content is high, all greener heart of vc, flavone, dietary fiber content, gold zone Fructus actinidiae chinensiss High.
It is clear that red kiwifruit highest from nutritive value, but one side Kiwi fresh fruit to preserve difficulty higher, separately On the one hand its sugar-acid ratio is higher, needs the crowd of glucose-diet unfavorable part, because it is processed further improving to it The hold capacity of red kiwifruit and nutrient utilization.
Enzyme liquid be organic vegetable, fruit, herbaceous plant under given conditions, obtain through fermentation after a while Liquid.The form that ferment exists has liquid, mastic and powder etc..Enzyme liquid have high activity, easily absorption, antiinflammatory, antibacterial, The features such as purify, decompose digestion, in good taste, favourable to health.
Content of the invention
It is an object of the invention to provide a kind of reduce red kiwifruit sugar content, increase the red heart of its nutrient utilization The production method of Fructus actinidiae chinensiss enzyme liquid, this method can be in the situation keeping the high solid content of red kiwifruit, high dietary-fiber Under low temperature fermentation is carried out to it, thus avoiding the wherein oxidation loss of vc.
Technical scheme is as follows:
A kind of production method of red kiwifruit enzyme liquid, comprises the following steps:
(1) select colory red kiwifruit fresh fruit, cleaning blends at a high speed after removing mud, fruit juice of squeezing out;
(2) add pectase, cellulase, yeast into the sarcocarp of portion of residual, put in incubator at 40 ~ 45 DEG C Put 1 ~ 2 day, take out thereafter and add deionized water and be sufficiently stirred for, stand, separate supernatant;
(3) add sucrose and acetic acid bacteria in the supernatant obtaining, thereafter Deca in solid wort agar culture medium, in Cultivate at 30 ~ 40 DEG C 3 ~ 5 days, after pulverizing thereafter, addition deionized water are sufficiently stirred for, centrifugation, obtain supernatant, it is dense It is reduced to 1/10 ~ 1/4 volume, obtain base fluid;
(4) base fluid for 1 ~ 5wt% of fruit juice for the quality is added in fruit juice, in 5 ~ 10 DEG C of constant temperature bottom fermentation 10 ~ 20 days, thereafter By pasteurization, that is, obtain red kiwifruit enzyme liquid.
Preferred embodiment is: sarcocarp described in described step (2) and pectase, cellulase, yeast and last The mass ratio of the deionized water adding is 1:0.03 ~ 0.05%:0.01 ~ 0.02%:0.1 ~ 0.2%:0.8 ~ 1.5.
In addition preferred embodiment is: the sucrose that adds in described step (3), acetic acid bacteria and going of being eventually adding from The mass ratio of the supernatant obtaining in sub- water and step (2) is 20 ~ 30%:0.05 ~ 0.2%:0.8 ~ 1:1.
In addition preferred embodiment is: is initially charged cellulase and yeast in described step (2), after placing 6 ~ 12h Add pectase.
In addition preferred embodiment is: adds 0.5% ~ 1.5% system in described step (4) while adding base fluid Good red kiwifruit enzyme liquid.
The present invention can carry out the prepared enzyme liquid that ferments at low temperature to red kiwifruit, increases the nutrition profit of red kiwifruit With rate, decrease the destruction to nutritional labelings other in red kiwifruit, especially decrease the destruction to vc, prepared enzyme liquid The content compared with vc in Kiwi fresh fruit for the content of middle vc descends below 10%.
Specific embodiment
With reference to embodiment, the present invention is further detailed:
Embodiment 1
(1) select colory red kiwifruit fresh fruit, cleaning blends at a high speed after removing mud, fruit juice of squeezing out;
(2) squeeze the juice to 10kg and add 3g pectase, 1g cellulase, 10g yeast in remaining sarcocarp, in training at 40 DEG C Place in foster case 2 days, take out thereafter and add 8kg deionized water and be sufficiently stirred for, stand, separate supernatant;
(3) 1kg sucrose and 2.5g acetic acid bacteria are added in 5kg supernatant, Deca is in solid wort agar culture medium thereafter On, Deca 0.8kg on every kg broth, cultivate at 30 DEG C 5 days, pulverize thereafter, add 4kg deionized water to be sufficiently stirred for Afterwards, centrifugation, obtains supernatant, is concentrated into 1/10 volume, obtains base fluid;
(4) 0.1kg base fluid is added in 10kg fruit juice, in 10 DEG C of constant temperature bottom fermentation 10 days, sterilize thereafter, that is, obtain red heart Mi Monkey Fructus Persicae enzyme liquid.
In the red kiwifruit enzyme liquid obtaining, flavones content and the flavones content in fresh Fructus actinidiae chinensiss maintain an equal level, and vc content is relatively Fresh Fructus actinidiae chinensiss decline 9.1%.
Embodiment 2
(1) select colory red kiwifruit fresh fruit, cleaning blends at a high speed after removing mud, fruit juice of squeezing out;
(2) 5g pectase, 2g cellulase, 20g yeast are added into 10kg remnants sarcocarp, at 45 DEG C in incubator Place 1 day, take out thereafter and add 15kg deionized water and be sufficiently stirred for, stand, separate supernatant;
(3) 1.5kg sucrose and 10g acetic acid bacteria are added in 5kg supernatant, Deca is in solid wort agar culture medium thereafter On, Deca 0.5kg on every kg broth, cultivate at 40 DEG C 3 days, pulverize thereafter, add 5kg deionized water to be sufficiently stirred for Afterwards, centrifugation, obtains supernatant, is concentrated into 1/4 volume, obtains base fluid;
(4) 0.5kg base fluid is added in 10kg fruit juice, in 5 DEG C of constant temperature bottom fermentation 20 days, sterilize thereafter, that is, obtain red heart Mi Monkey Fructus Persicae enzyme liquid.
In the red kiwifruit enzyme liquid obtaining, flavones content and the flavones content in fresh Fructus actinidiae chinensiss maintain an equal level, and vc content is relatively Fresh Fructus actinidiae chinensiss decline 8%.
Embodiment 3
(1) select colory red kiwifruit fresh fruit, cleaning blends at a high speed after removing mud, fruit juice of squeezing out;
(2) add 2g cellulase, 20g yeast into 10kg remnants sarcocarp, at 40 DEG C, place 6h in incubator, its Afterwards add 5g pectase, at 45 DEG C in incubator place 36h, thereafter take out add 8kg deionized water and be sufficiently stirred for, quiet Put, separate supernatant;
(3) 1.2kg sucrose and 10g acetic acid bacteria are added in 5kg supernatant, Deca is in solid wort agar culture medium thereafter On, Deca 0.8kg on every kg broth, cultivate at 35 DEG C 4 days, pulverize thereafter, add 5kg deionized water to be sufficiently stirred for Afterwards, centrifugation, obtains supernatant, is concentrated into 1/5 volume, obtains base fluid;
(4) 0.5kg base fluid is added in 10kg fruit juice, in 6 DEG C of constant temperature bottom fermentation 12 days, sterilize thereafter, that is, obtain red heart Mi Monkey Fructus Persicae enzyme liquid.
In the red kiwifruit enzyme liquid obtaining, flavones content and the flavones content in fresh Fructus actinidiae chinensiss maintain an equal level, and vc content is relatively Fresh Fructus actinidiae chinensiss decline 5.1%, and the red kiwifruit enzyme liquid that sugar content obtains relative to embodiment 2 declines 10%.
Embodiment 4
(1) select colory red kiwifruit fresh fruit, cleaning blends at a high speed after removing mud, fruit juice of squeezing out;
(2) add 2g cellulase, 20g yeast into 10kg remnants sarcocarp, at 45 DEG C, place 12h in incubator, its Afterwards add 5g pectase, at 40 DEG C in incubator place 36h, thereafter take out add 8kg deionized water and be sufficiently stirred for, quiet Put, separate supernatant;
(3) 1.5kg sucrose and 10g acetic acid bacteria are added in 5kg supernatant, Deca is in solid wort agar culture medium thereafter On, Deca 0.8kg on every kg broth, cultivate at 30 DEG C 5 days, pulverize thereafter, add 5kg deionized water to be sufficiently stirred for Afterwards, centrifugation, obtains supernatant, is concentrated into 1/5 volume, obtains base fluid;
(4) 0.1kg base fluid is added in 10kg fruit juice with produced red kiwifruit enzyme liquid 0.05kg, in 8 DEG C of constant temperature Bottom fermentation 15 days, sterilizes thereafter, that is, obtain red kiwifruit enzyme liquid.
In the red kiwifruit enzyme liquid obtaining, flavones content and the flavones content in fresh Fructus actinidiae chinensiss maintain an equal level, and vc content is relatively Fresh Fructus actinidiae chinensiss decline 5%, and the red kiwifruit enzyme liquid that total acid content obtains with respect to embodiment 2 improves 13%.
Although reference be made herein to invention has been described for the explanatory embodiment of the present invention, and above-described embodiment is only this Bright preferably embodiment, embodiments of the present invention are simultaneously not restricted to the described embodiments it should be appreciated that people in the art Member can be designed that a lot of other modifications and embodiment, and these modifications and embodiment will fall in principle disclosed in the present application Within scope and spirit.

Claims (5)

1. a kind of production method of red kiwifruit enzyme liquid it is characterised in that: comprise the following steps:
(1) select colory red kiwifruit fresh fruit, cleaning blends at a high speed after removing mud, fruit juice of squeezing out;
(2) add pectase, cellulase, yeast into the sarcocarp of portion of residual, put in incubator at 40 ~ 45 DEG C Put 1 ~ 2 day, take out thereafter and add deionized water and be sufficiently stirred for, stand, separate supernatant;
(3) add sucrose and acetic acid bacteria in the supernatant obtaining, thereafter Deca in solid wort agar culture medium, in Cultivate at 30 ~ 40 DEG C 3 ~ 5 days, after pulverizing thereafter, addition deionized water are sufficiently stirred for, centrifugation, obtain supernatant, it is dense It is reduced to 1/10 ~ 1/4 volume, obtain base fluid;
(4) base fluid for 1 ~ 5wt% of fruit juice for the quality is added in fruit juice, in 5 ~ 10 DEG C of constant temperature bottom fermentation 10 ~ 20 days, thereafter Through pasteurization, that is, obtain red kiwifruit enzyme liquid.
2. red kiwifruit enzyme liquid according to claim 1 production method it is characterised in that: in described step (2) Described sarcocarp is 1:0.03 ~ 0.05% with the mass ratio of pectase, cellulase, yeast and the deionized water being eventually adding: 0.01 ~ 0.02%:0.1 ~ 0.2%:0.8 ~ 1.5.
3. red kiwifruit enzyme liquid according to claim 1 production method it is characterised in that: in described step (3) The mass ratio of the supernatant obtaining in the sucrose that adds, acetic acid bacteria and the deionized water being eventually adding and step (2) for 20 ~ 30%:0.05 ~ 0.2%:0.8 ~ 1:1.
4. red kiwifruit enzyme liquid according to claim 1 production method it is characterised in that: in described step (2) It is initially charged cellulase and yeast, after placing 6 ~ 12h, add pectase.
5. red kiwifruit enzyme liquid according to claim 1 production method it is characterised in that: in described step (4) Add 0.5% ~ 1.5% produced red kiwifruit enzyme liquid while adding base fluid.
CN201610746845.4A 2016-08-29 2016-08-29 Production method for red-fleshed kiwifruit ferment liquid Pending CN106360216A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107549566A (en) * 2017-09-27 2018-01-09 河南工业大学 A kind of Kiwi berry solid beverage ferment and preparation method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10276736A (en) * 1997-04-09 1998-10-20 Okayama Pref Gov Shin Gijutsu Shinko Zaidan Production of fermented beverage containing acetic acid bacterium cellulose
CN101904504A (en) * 2010-08-10 2010-12-08 厦门金日制药有限公司 Five-element enzyme liquid and preparation method thereof
CN103305432A (en) * 2013-04-09 2013-09-18 杭州清正生物科技有限公司 Saccharomyces cerevisiae strain and application thereof
CN103609985A (en) * 2013-10-25 2014-03-05 晶叶(青岛)生物科技有限公司 Fermentation method for production of nutritionally balanced natural fruit enzyme
CN105166933A (en) * 2015-10-28 2015-12-23 青海尤尼克生物技术有限公司 Composition containing Chinese wolfberry fruit extract and fruit ferment and preparation method thereof
CN105211865A (en) * 2015-10-28 2016-01-06 青海尤尼克生物技术有限公司 A kind of black fruit lyceum ferment and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10276736A (en) * 1997-04-09 1998-10-20 Okayama Pref Gov Shin Gijutsu Shinko Zaidan Production of fermented beverage containing acetic acid bacterium cellulose
CN101904504A (en) * 2010-08-10 2010-12-08 厦门金日制药有限公司 Five-element enzyme liquid and preparation method thereof
CN103305432A (en) * 2013-04-09 2013-09-18 杭州清正生物科技有限公司 Saccharomyces cerevisiae strain and application thereof
CN103609985A (en) * 2013-10-25 2014-03-05 晶叶(青岛)生物科技有限公司 Fermentation method for production of nutritionally balanced natural fruit enzyme
CN105166933A (en) * 2015-10-28 2015-12-23 青海尤尼克生物技术有限公司 Composition containing Chinese wolfberry fruit extract and fruit ferment and preparation method thereof
CN105211865A (en) * 2015-10-28 2016-01-06 青海尤尼克生物技术有限公司 A kind of black fruit lyceum ferment and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107549566A (en) * 2017-09-27 2018-01-09 河南工业大学 A kind of Kiwi berry solid beverage ferment and preparation method thereof

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Application publication date: 20170201