CN106353493A - Ochratoxin test rod - Google Patents
Ochratoxin test rod Download PDFInfo
- Publication number
- CN106353493A CN106353493A CN201610834647.3A CN201610834647A CN106353493A CN 106353493 A CN106353493 A CN 106353493A CN 201610834647 A CN201610834647 A CN 201610834647A CN 106353493 A CN106353493 A CN 106353493A
- Authority
- CN
- China
- Prior art keywords
- parts
- ochratoxin
- pad
- sample
- agglomerated particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
Abstract
The invention discloses an ochratoxin test rod, and belongs to the field of a test paper. The ochratoxin test rod comprises an outer shell, wherein the bottom part of the outer shell is provided with a sample injection end, and the inner part of the outer shell is provided with a detection zone; the sample injection end is connected with the detection zone through a sensing zone, and a few amount of sensing agglomeration particles are filled in the sensing zone; the test paper is arranged in the detection zone. The ochratoxin test rod is convenient to use, and is quick and sensitive; sample is not required to carry out complex pretreatment, the ochratoxin test rod can rapidly test if the sample contains ochratoxin; the detection sensitivity is high, and the detection limit can reach 1.4 ng/mL.
Description
Technical field
The present invention relates to a kind of Test paper, particularly a kind of ochratoxin test bar.
Background technology
Mycotoxin be a kind of be prevalent in feedstuff and food by mycetogenetic secondary toxic metabolic products, main
Injury is produced to human body or animal by provand link, including causing common inflammatory reaction such as gastroenteropathy, to change huge
Phagocyte phenotype and the damage of dna, or even height carcinogenecity, and the reproductive system of human body or animal can be damaged, right
Upgrowth and development of children has and has a strong impact on.Mycotoxin contamination scope is wider, can be crops such as Semen Tritici aestivi, Semen Maydiss it is also possible to
It is fresh and living aquatic products, people use also or in daily life milk, coffee etc..The existing detection master to mycotoxin
Chromatography to be had, mass spectrography, lc-ms/ms etc., although these methods have high specificity and sensitivity, generally existing sample
Product complex pretreatment is loaded down with trivial details, time-consuming, be not suitable for the weak points such as execute-in-place.
Content of the invention
The goal of the invention of the present invention is: for above-mentioned problem, provide a kind of convenient use, rapid sensitive, no
Complicated pre-treatment need to be carried out to sample, can quickly detect to whether containing ochratoxin in sample, detection sensitivity
Higher, detection limitation can 1.4ng/ml ochratoxin test bar.
The technical solution used in the present invention is as follows:
A kind of ochratoxin test bar of the present invention, including shell, described outer casing bottom is provided with sample introduction end, described enclosure
It is provided with detection zone, described sample introduction end is connected by induction zone with detection zone, in described induction zone, be filled with a small amount of sensing reunion grain
Son, is provided with Test paper in described detection zone, and described upper surface of outer cover is provided with form, described form above detection zone, institute
State distance >=8.9cm apart from detection zone for the induction zone.
Due to employing technique scheme, mycotoxin is entered by sample collecting, then through agglomerated particles by sample introduction end
Row amplifies, and carries out aptamers screening finally by Test paper to mycotoxin, thus realizing the malicious to Aspergillus ochraceus of rapid sensitive
Element is detected；Above-mentioned induction zone can ensure agglomerated particles as far as possible by ochratoxin group apart from the distance of detection zone
Poly-, thus ensureing high sensitivity.
A kind of ochratoxin test bar of the present invention, is provided with two passes, respectively passage one He in described induction zone
Passage two, has agglomerated particles a in described passage one, has agglomerated particles b in described passage two, described agglomerated particles a and group
All by amylan gluing on conduit wall, the gross mass of described agglomerated particles a is 0.43mg to poly- particle b, described agglomerated particles b
Gross mass be 0.07mg.
Due to employing technique scheme, agglomerated particles are reunited rapidly by ochratoxin through agglomerated particles, thus
Realize ochratoxin is amplified, by two kinds of different agglomerated particles it is ensured that the agglomerating effect of contratoxin, it is to avoid missing inspection.
A kind of ochratoxin test bar of the present invention, is provided with suction tube in described sample introduction end, described suction tube includes setting
In the air bag at top, described air bag is connected with suction pipe, and described air bag is made up of elastomeric material, has deionization in described air bag
Water；Described suction tube bottom is provided with two outlets, and described two outlets are agreed with passage one and passage two-phase respectively.
Due to employing technique scheme, by suction tube leading portion, sample is acquired, extruding gasbag can be by gas
In capsule storage deionized water by diluted sample, consequently facilitating agglomerated particles are reunited with ochratoxin, sample is dilute
After releasing, standing 10～15min presses pressuring gasbag again, sample is pushed through induction zone, reaches detection zone, completes detection colour developing.
A kind of ochratoxin test bar of the present invention, described Test paper has layer structure, described Test paper bag
Include the matrix being placed in bottom, above described matrix in the middle of be provided with that detecting pad described in detecting pad is cup-shaped, described detecting pad side sets
There is overflow prevention pad, described detecting pad opposite side is provided with leaching sample pad, described detecting pad upper end-face edge is all overlying on overflow prevention pad and leaching sample pad
Surface, described overflow prevention pad upper surface is covered with colour developing film, and described colour developing film is in same level with the upper end-face edge of detecting pad,
Described colour developing film is spaced with detecting pad, and described leaching sample pad upper surface is covered with suction batten, and described suction batten is connected with induction zone.
Due to employing technique scheme, after sample liquid being absorbed by suction batten, then absorb sample by soaking sample pad
Liquid, thus simply being separated to the sample in sample liquid, large volume of histiocyte etc. is filtered, it is to avoid tissue
Cell impacts to testing result, and the immersional wetting through leaching sample pad ensure that sample flows through flow velocity during detecting pad simultaneously
Uniformly so that sample is fully combined with aptamers, mix homogeneously, then colour developing film is finally flowed into by immersional wetting, thus completing
Uniformly develop the color, it is to avoid color distortion in the test result obtaining on colour developing film, ensure accuracy of detection and accuracy of observation simultaneously.
A kind of ochratoxin test bar of the present invention, described agglomerated particles a with Nanometer Copper as inner core, uniformly wrap up by surface
There is carbonization Bacterial cellulose, with nanometer cobalt as inner core, surface is uniformly enclosed with amycin to described agglomerated particles b.
Due to employing technique scheme, the agglomerating effect of agglomerated particles is good, and detectivity is high, reunion grain simultaneously
Footpath suitable size, will not granule be excessive filters out in detection zone immersed sample pad.
A kind of ochratoxin test bar of the present invention, described detecting pad surface is evenly coated with aptamers layer, described adaptation
Body layer is by 37 parts of aptamers of mass parts, 22 parts of gold chlorides, 27 parts of trisodium citrates and 16 parts of fourth sand amine alcohol compositions, described core
Calculation aptamers sequence is 5 '-gatcgggtgtgggtggcgtaaagggagcatcggacaagaaatgccaca-3 '.
Due to employing technique scheme, this aptamers is high to the affinity of ochratoxin, and the end of the chain has strepto- parent
And element, ochratoxin can be captured rapidly.
A kind of ochratoxin test bar of the present invention, described Test paper surface is coated with barrier film, described barrier film one end
It is fixed on matrix lower surface, the other end of described barrier film is in being fixedly connected in inhaling batten front end.
Due to employing technique scheme, barrier film is made up of waterproof material, thus ensureing that the hydrone of in the air will not
Impact detection colour developing result.
A kind of ochratoxin test bar of the present invention, by 13 parts of nano Au particles of mass parts, 0.7 part is coagulated described colour developing film
Hemase antibody, 1 part of quartz crystal, 8 parts of mercaptosilane coupling agents and 8 parts of Folic Acid are made, described nano Au particle a diameter of
16nm.
Due to employing technique scheme, colour developing film takes on a red color in normal state, will when ochratoxin is detected
Become au bleu, by coupling agent by Folic Acid, quartz crystal and nano Au particle carry out crosslinking, can strengthen the aobvious of nano Au particle
Color effect, color developing effect can improve 10 times compared with prior art, and color change substantially, improves detectable limit.
A kind of ochratoxin test bar of the present invention, described matrix is by 45 parts of sio of mass parts2, 12 parts of 4- (4- aminobenzenes
Epoxide) pyridine, 22 parts of polyesteramides and 16 parts of n, n- DMAA makes, and described leaching sample pad and overflow prevention pad are by mass parts
72 parts of propylene lactic acid, 78 parts of starch-grafted polyacrylonitrile, 3 parts of zno and 9 part of plastic of poly vinyl acetate compositions, described suction batten
By 30 parts of starch-grafted polyacrylonitrile, 47 parts of ethylene glycol dimethacrylates, 16 parts of polymethyl acrylate, 20 parts of metering systems
Sour methyl ester, 13 parts of vinyl acetates and 7 parts of sodium lauryl sulphates are made.
Due to employing technique scheme, matrix properties are stable, and the wetting property of leaching sample pad and overflow prevention pad is good, can
Ensure sample will not slime flux, it is high to inhale batten sensitivity, sample can be avoided to retain in induction zone sample absorbance rapidly, affect
Detection limitation.
In sum, due to employing technique scheme, the invention has the beneficial effects as follows:
1st, convenient use, rapid sensitive, complicated pre-treatment need not be carried out to sample, can be quickly to whether containing reddish brown song in sample
Mould toxin is detected, detection sensitivity is higher, and detection limitation can 1.4ng/ml.
2nd, the sample in sample liquid simply can be separated, large volume of histiocyte etc. is filtered,
Avoid histiocyte that testing result is impacted, the flow velocity that sample flows through during detecting pad is uniform, colour developing is uniformly, it is to avoid in colour developing
Color distortion in the test result obtaining on film, ensures accuracy of detection and accuracy of observation simultaneously.
Brief description
Fig. 1 is a kind of ochratoxin test bar structural representation；
Fig. 2 is Test paper structural representation.
In figure labelling: 1 is shell, 2 is sample introduction end, and 3 is air bag, and 4 is suction tube, and 5 is induction zone, and 6 is detection zone, and 7 are
Matrix, 8 is overflow prevention pad, and 9 is leaching sample pad, and 10 is to inhale batten, and 11 is colour developing film, and 12 is detecting pad, and 13 is barrier film.
Specific embodiment
Below in conjunction with the accompanying drawings, the present invention is described in detail.
In order that the purpose of invention, technical scheme and advantage become more apparent, below in conjunction with drawings and Examples, to this
Invention is further elaborated.It should be appreciated that specific embodiment described herein is only in order to explain the present invention, not
For limiting the present invention.
Embodiment 1
As shown in Figure 1 to Figure 2, a kind of ochratoxin test bar, including shell 1, shell 1 bottom is provided with sample introduction end 2, shell 1
Inside is provided with detection zone 6, and sample introduction end 2 is connected by induction zone 5 with detection zone 6, is filled with a small amount of agglomerated particles in induction zone 5,
It is provided with Test paper, shell 1 upper surface is provided with form, above detection zone 6, induction zone 5 is apart from detection for form in detection zone 6
Distance >=the 8.9cm in area 6.It is provided with two passes, respectively passage one and passage two in induction zone 5, in passage one, have reunion
Particle a, has agglomerated particles b in passage two, agglomerated particles a and agglomerated particles b all by amylan gluing on conduit wall,
The gross mass of agglomerated particles a is 0.43mg, and the gross mass of agglomerated particles b is 0.07mg.It is provided with suction tube 4 in sample introduction end 2, inhale sample
Pipe 4 includes the air bag 3 located at top, and air bag 3 is connected with suction pipe, and air bag 3 is made up of elastomeric material, has deionization in air bag 3
Water；Suction tube 4 bottom is provided with two outlets, and two outlets are agreed with passage one and passage two-phase respectively.
Test paper has layer structure, and Test paper includes the matrix 7 being placed in bottom, and matrix 7 is provided with inspection in the middle of top
Detecting pad described in survey pad 12 is cup-shaped, and detecting pad 12 side is provided with overflow prevention pad 8, and described detecting pad 12 opposite side is provided with leaching sample pad 9,
Described detecting pad 12 upper end-face edge is all overlying on overflow prevention pad 8 and leaching sample pad 9 upper surface, and overflow prevention pad 8 upper surface is covered with colour developing film 11, shows
Color film 11 is in same level with the upper end-face edge of detecting pad 12, and colour developing film 11 is spaced with detecting pad 12, soaks sample pad 9 upper table
Face is covered with suction batten 10, inhales batten 10 and is connected with induction zone 5.Test paper surface is coated with barrier film 13, and barrier film 13 one end is fixed
In matrix 9 lower surface, the other end of barrier film 13 is in being fixedly connected in inhaling batten 10 front end.
With Nanometer Copper as inner core, surface is uniformly enclosed with carbonization Bacterial cellulose to agglomerated particles a, and agglomerated particles b are with nanometer
Cobalt is inner core, and surface is uniformly enclosed with amycin.Detecting pad 12 surface is evenly coated with aptamers layer, and aptamers layer is by mass parts 37
Part aptamer, 22 parts of gold chlorides, 27 parts of trisodium citrates and 16 parts of fourth sand amine alcohols form, and described accounting aptamers sequence is
5’-gatcgggtgtgggtggcgtaaagggagcatcggacaagaaatgccaca-3’.Colour developing film 11 is by 13 parts of mass parts
Nano Au particle, 0.7 part of anti-thrombin antibody, 1 part of quartz crystal, 8 parts of mercaptosilane coupling agents and 8 parts of Folic Acid are made, nanometer gold
A diameter of 16nm of particle.Matrix is by 45 parts of sio of mass parts2, 12 parts of 4- (4- amino-benzene oxygen) pyridines, 22 parts of polyesteramides and
16 parts of n, n- DMAA is made, and, by 72 parts of propylene lactic acid of mass parts, 78 parts of starch connect for described leaching sample pad and overflow prevention pad
Branch polyacrylonitrile, 3 parts of zno and 9 part of plastic of poly vinyl acetate composition, described suction batten by 30 parts of starch-grafted polyacrylonitrile,
47 parts of ethylene glycol dimethacrylates, 16 parts of polymethyl acrylate, 20 parts of methyl methacrylates, 13 parts of vinyl acetates and 7
Part sodium lauryl sulphate is made.
Embodiment 2
The preparation method of agglomerated particles a:
The Bacterial cellulose cleaned is immersed in after 12h in 4% copper-bath and takes out, wherein Bacterial cellulose and copper sulfate
The ratio of the amount of material is 8:1, puts in the dobell's solution of nitrogen protection under agitation, dobell's solution is by amycin
It is completely soaked, according to the ratio copper sulfate of the amount of material: sodium borate: ascorbic acid=1:1:0.7 is slowly added dropwise boric acid in solution
Sodium, after 35 DEG C of reaction 30min, adds ascorbic acid in solution, after standing 1 ~ 2h, above-mentioned product deionized water is rinsed
For several times, lyophilized overnight prepares sample.
Embodiment 3
The preparation method of agglomerated particles b is as follows:
Amycin is immersed in after 12h in 4% cobalt chloride solution and takes out, wherein amycin and the ratio of the amount of cobaltous chloride material are
12:1, puts in the sodium hydroxide solution of nitrogen protection, amycin is completely soaked by sodium hydroxide solution under agitation,
Ratio cobaltous chloride according to the amount of material: sodium hydroxide: ascorbic acid=1:1:0.7 is slowly added dropwise sodium hydroxide in solution, 35
DEG C reaction 30min after, in solution add ascorbic acid, standing 1 ~ 2h after, by above-mentioned product deionized water rinse for several times, cold
Freeze to be dried overnight and prepare sample.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Any modification, equivalent and improvement of being made within principle etc., should be included within the scope of the present invention.
Claims (9)
1. a kind of ochratoxin test bar it is characterised in that: include shell (1), described shell (1) bottom is provided with sample introduction end
(2), it is provided with detection zone (6) inside described shell (1), described sample introduction end (2) is connected by induction zone (5) with detection zone (6), institute
State and in induction zone (5), be filled with a small amount of agglomerated particles, in described detection zone (6), be provided with Test paper, described shell (1) upper surface
It is provided with form, above detection zone (6), described induction zone (5) is apart from the distance >=8.9cm of detection zone (6) for described form.
2. as claimed in claim 1 a kind of ochratoxin test bar it is characterised in that: be provided with two in described induction zone (5)
Bar passage, respectively passage one and passage two, have agglomerated particles a in described passage one, have reunion grain in described passage two
Sub- b, described agglomerated particles a and agglomerated particles b all by amylan gluing on conduit wall, the gross mass of described agglomerated particles a
For 0.43mg, the gross mass of described agglomerated particles b is 0.07mg.
3. required by right a kind of ochratoxin test bar as described in 2 it is characterised in that: be provided with suction in described sample introduction end (2)
Sample pipe (4), described suction tube (4) includes the air bag (3) located at top, and described air bag (3) is connected with suction pipe, described air bag (3)
It is made up of elastomeric material, in described air bag (3), have deionized water；Described suction tube (4) bottom is provided with two outlets, described scene 2
Mouth is agreed with passage one and passage two-phase respectively.
4. as claimed in claim 2 or claim 3 a kind of ochratoxin test bar it is characterised in that: described Test paper has layer
Shape structure, described Test paper includes the matrix (7) being placed in bottom, and above described matrix (7), centre is provided with detecting pad (12) institute
State detecting pad cup-shaped, described detecting pad (12) side is provided with overflow prevention pad (8), described detecting pad (12) opposite side is provided with leaching sample pad
(9), described detecting pad (12) upper end-face edge is all overlying on overflow prevention pad (8) and leaching sample pad (9) upper surface, described overflow prevention pad (8) upper table
Face is covered with colour developing film (11), and described colour developing film (11) is in same level with the upper end-face edge of detecting pad (12), described aobvious
Color film (11) is spaced with detecting pad (12), and described leaching sample pad (9) upper surface is covered with suction batten (10), described suction batten (10) and sense
Area (5) is answered to be connected.
5. as claimed in claim 4 a kind of ochratoxin test bar it is characterised in that: described agglomerated particles a are with Nanometer Copper
For inner core, surface is uniformly enclosed with carbonization Bacterial cellulose, and described agglomerated particles b with nanometer cobalt as inner core, uniformly wrap up by surface
There is amycin.
6. as claimed in claim 5 a kind of ochratoxin test bar it is characterised in that: described detecting pad (12) surface is uniform
Scribble aptamers layer, described aptamers layer is by 37 parts of aptamers of mass parts, 22 parts of gold chlorides, 27 parts of trisodium citrates and 16
Part fourth sand amine alcohol composition, described accounting aptamers sequence is 5 '-gatcgggtgtgggtggcgtaaagggagcatcggacaaga
aatgccaca-3’.
7. a kind of ochratoxin test bar as described in claim 5 or 6 it is characterised in that: described Test paper surface is covered
It is stamped barrier film (13), matrix (9) lower surface is fixed in described barrier film (13) one end, and the other end of described barrier film (13) is in suction sample
Bar (10) front end is fixedly connected.
8. as claimed in claim 7 a kind of ochratoxin test bar it is characterised in that: described colour developing film (11) by mass parts
13 parts of nano Au particles, 0.7 part of anti-thrombin antibody, 1 part of quartz crystal, 8 parts of mercaptosilane coupling agents and 8 parts of Folic Acid are made, institute
State a diameter of 16nm of nano Au particle.
9. as claimed in claim 8 a kind of ochratoxin test bar it is characterised in that: described matrix is by 45 parts of mass parts
sio2, 12 parts of 4- (4- amino-benzene oxygen) pyridines, 22 parts of polyesteramides and 16 parts of n, n- DMAA is made, described leaching
Sample pad and overflow prevention pad are by 72 parts of propylene lactic acid of mass parts, 78 parts of starch-grafted polyacrylonitrile, 3 parts of zno and 9 part of polyvinyl acetate second
Alkene ester forms, and described suction batten is by 30 parts of starch-grafted polyacrylonitrile, 47 parts of ethylene glycol dimethacrylates, 16 parts of polypropylene
Sour methyl ester, 20 parts of methyl methacrylates, 13 parts of vinyl acetates and 7 parts of sodium lauryl sulphates are made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610834647.3A CN106353493A (en) | 2016-09-21 | 2016-09-21 | Ochratoxin test rod |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610834647.3A CN106353493A (en) | 2016-09-21 | 2016-09-21 | Ochratoxin test rod |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106353493A true CN106353493A (en) | 2017-01-25 |
Family
ID=57858510
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610834647.3A Pending CN106353493A (en) | 2016-09-21 | 2016-09-21 | Ochratoxin test rod |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106353493A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006886A (en) * | 2019-04-24 | 2019-07-12 | 江苏大学 | A kind of nanosizing color sensitive sensor and its method for differentiating wheat moulding ability |
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| CN101231290A (en) * | 2007-01-25 | 2008-07-30 | 天津科技大学 | Kit and method for quantitative determination of ochratoxin A content in food product |
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| CN101231290A (en) * | 2007-01-25 | 2008-07-30 | 天津科技大学 | Kit and method for quantitative determination of ochratoxin A content in food product |
| CN102854310A (en) * | 2011-12-27 | 2013-01-02 | 深圳市爱速尔生物技术有限公司 | Living-animal food safety rapid detector |
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
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| WO2016094722A1 (en) * | 2014-12-10 | 2016-06-16 | Xpecting Diagnostics, Inc. | Detection of analytes in bodily fluid to determine the initiation of parturition |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110006886A (en) * | 2019-04-24 | 2019-07-12 | 江苏大学 | A kind of nanosizing color sensitive sensor and its method for differentiating wheat moulding ability |
| CN110006886B (en) * | 2019-04-24 | 2022-01-11 | 江苏大学 | Nanocrystallization color-sensitive sensor and method for judging wheat mildew degree by using same |
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Application publication date: 20170125 |