CN106248926A - A kind of mycotoxin Test paper - Google Patents
A kind of mycotoxin Test paper Download PDFInfo
- Publication number
- CN106248926A CN106248926A CN201610835146.7A CN201610835146A CN106248926A CN 106248926 A CN106248926 A CN 106248926A CN 201610835146 A CN201610835146 A CN 201610835146A CN 106248926 A CN106248926 A CN 106248926A
- Authority
- CN
- China
- Prior art keywords
- parts
- pad
- test paper
- mycotoxin
- detecting pad
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
- G01N2333/38—Assays involving biological materials from specific organisms or of a specific nature from fungi from Aspergillus
Abstract
The invention discloses a kind of mycotoxin Test paper, belong to Test paper field.A kind of mycotoxin Test paper of the present invention, including the matrix being placed in bottom, it is provided with detecting pad described in detecting pad cup-shaped in the middle of above described matrix, described detecting pad side is provided with overflow prevention pad, described detecting pad opposite side is provided with leaching sample pad, and described detecting pad upper end-face edge is all overlying on overflow prevention pad and leaching sample pad upper surface, and described overflow prevention pad upper surface is covered with colour developing film, described leaching sample pad upper surface is covered with suction batten, and described suction batten is connected with induction zone.A kind of mycotoxin Test paper of the present invention has convenient use, rapid sensitive, it is not necessary to sample carries out complicated pre-treatment, it is possible to quickly detecting whether contain ochratoxin in sample, detection sensitivity is higher, and detection is limited the quantity the feature up to 1.4ng/mL.
Description
Technical field
The present invention relates to a kind of Test paper, particularly a kind of mycotoxin Test paper.
Background technology
Mycotoxin is a kind of by mycetogenetic secondary toxic metabolic products, is prevalent in feedstuff and food, master
Provand link to be passed through produces injury to human body or animal, including causing common inflammatory reaction such as gastroenteropathy, changes huge
Phagocyte phenotype and the damage of DNA, even height carcinogenecity, and the reproductive system of human body or animal can be damaged, right
Upgrowth and development of children has and has a strong impact on.Mycotoxin contamination scope is relatively wide, can be crops such as Semen Tritici aestivis, Semen Maydis, it is also possible to
It is fresh and living aquatic products, people use also or in daily life milk, coffee etc..The existing detection master to mycotoxin
, although these methods have high specificity and sensitivity, but generally there is sample in chromatography to be had, mass spectrography, LC-MS/MS etc.
Product complex pretreatment length loaded down with trivial details, time-consuming, be not suitable for the weak points such as execute-in-place.
Summary of the invention
The goal of the invention of the present invention is: for the problem of above-mentioned existence, it is provided that a kind of convenient use, rapid sensitive, nothing
Sample need to be carried out complicated pre-treatment, it is possible to quickly detect whether sample contains ochratoxin, detection sensitivity
Higher, detection limitation can the mycotoxin Test paper of 1.4ng/mL.
The technical solution used in the present invention is as follows:
A kind of mycotoxin Test paper of the present invention, including the matrix bottom being placed in, above described matrix, centre is provided with detection
Padding described detecting pad cup-shaped, described detecting pad side is provided with overflow prevention pad, and described detecting pad opposite side is provided with leaching sample pad, described inspection
Surveying pad upper end-face edge and be all overlying on overflow prevention pad and leaching sample pad upper surface, described overflow prevention pad upper surface is covered with colour developing film, described leaching sample pad
Upper surface is covered with suction batten, and described suction batten is connected with induction zone.
Owing to have employed technique scheme, after sample liquid being absorbed by suction batten, then absorb sample by leaching sample pad
Liquid, thus the sample in sample liquid is simply separated, large volume of histiocyte etc. is filtered, it is to avoid tissue
Testing result is impacted by cell, and the immersional wetting through leaching sample pad ensure that sample flows through the flow velocity of detecting pad simultaneously
Uniformly so that sample is fully combined with aptamers, mix homogeneously, then finally flow into colour developing film by immersional wetting, thus complete
Uniformly colour developing, it is to avoid color distortion occurs in the test result obtained on colour developing film, ensures accuracy of detection and accuracy of observation simultaneously.
A kind of mycotoxin Test paper of the present invention, described colour developing film is in same level with the upper end-face edge of detecting pad
On face, described colour developing film and detecting pad interval.
A kind of mycotoxin Test paper of the present invention, the end face of described suction batten is higher than the upper end-face edge of detecting pad, institute
State suction batten and detecting pad interval.
Owing to have employed technique scheme, interval ensure that sample only enters detecting pad/colour developing by immersional wetting
Film, it is ensured that the precision of detection/colour developing.
A kind of mycotoxin Test paper of the present invention, described Test paper surface is coated with barrier film, described barrier film one end
Being fixed on matrix lower surface, the other end of described barrier film is fixing with suction batten front end to be connected.
Owing to have employed technique scheme, barrier film is made up of waterproof material, thus ensures that the hydrone in air will not
Impact detection colour developing result.
A kind of mycotoxin Test paper of the present invention, described suction spline surfaces is evenly coated with agglomerated particles, described reunion
Particle is with Nanometer Copper as inner core, and surface is uniformly enclosed with carbonization Bacterial cellulose.
Owing to have employed technique scheme, through agglomerated particles, mycotoxin is amplified, finally by Test paper
Mycotoxin is carried out aptamers screening, thus realizes rapid sensitive ochratoxin is detected, the group of agglomerated particles
Poly-respond well, detectivity is high, aggregated particle size suitable size simultaneously, will not granule is excessive filters at detection zone immersed sample pad
Fall.
A kind of mycotoxin Test paper of the present invention, described detecting pad surface is evenly coated with aptamers layer, described
Aptamers layer is by 37 parts of aptamers of mass parts, 22 parts of gold chlorides, 27 parts of trisodium citrates and 16 parts of fourth sand amine alcohol compositions, institute
Stating accounting aptamers sequence is 5 '-GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACAAGAAATGCCACA-3 '.
Owing to have employed technique scheme, this aptamers is high to the affinity of ochratoxin, and the end of the chain has strepto-parent
And element, it is possible to capture rapidly ochratoxin.
A kind of mycotoxin Test paper of the present invention, described colour developing film is by 13 parts of nano Au particles of mass parts, and 0.7 part is coagulated
Hemase antibody, 1 part of quartz crystal, 8 parts of mercaptosilane coupling agents and 8 parts of folic acid are made, described nano Au particle a diameter of
16nm。
Owing to have employed technique scheme, colour developing film takes on a red color at normal state, will when ochratoxin being detected
Become blue, by coupling agent, folic acid, quartz crystal are cross-linked with nano Au particle, it is possible to strengthen the aobvious of nano Au particle
Chromatic effect, color developing effect relatively prior art can improve 10 times, and color change substantially, improves detectable limit.
A kind of mycotoxin Test paper of the present invention, described matrix is by 45 parts of SiO of mass parts2, 12 parts of 4-(4-aminobenzenes
Epoxide) pyridine, 22 parts of polyesteramides and 16 parts of N,N-DMAAs are made.
A kind of mycotoxin Test paper of the present invention, described leaching sample pad and overflow prevention pad by 72 parts of propylene lactic acid of mass parts, 78 parts
Starch-grafted polyacrylonitrile, 3 parts of ZnO and 9 parts of plastic of poly vinyl acetate compositions.
A kind of mycotoxin Test paper of the present invention, it is characterised in that: described suction batten is by 30 parts starch-grafted poly-third
Alkene nitrile, 47 parts of ethylene glycol dimethacrylates, 16 parts of polymethyl acrylate, 20 parts of methyl methacrylates, 13 parts of acetic acid second
Alkene and 7 parts of sodium lauryl sulphates are made.
Owing to have employed technique scheme, matrix properties is stable, and the wetting property of leaching sample pad and overflow prevention pad is good, it is possible to
Ensure sample will not slime flux, inhale batten highly sensitive, it is possible to avoid sample to retain in induction zone sample absorbance rapidly, affect
Detection limitation.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
1, convenient use, rapid sensitive, it is not necessary to sample is carried out complicated pre-treatment, it is possible to quickly to whether containing reddish brown song in sample
Mould toxin detects, and detection sensitivity is higher, and detection limitation can 1.4ng/mL.
2, the sample in sample liquid simply can be separated, large volume of histiocyte etc. is filtered,
Avoiding histiocyte to impact testing result, flow velocity when sample flows through detecting pad is uniform, and colour developing is uniformly, it is to avoid in colour developing
There is color distortion in the test result obtained on film, ensures accuracy of detection and accuracy of observation simultaneously.
Accompanying drawing explanation
Fig. 1 is a kind of mycotoxin Test paper structural representation.
Labelling in figure: 7 is matrix, 8 is overflow prevention pad, and 9 is leaching sample pad, and 10 for inhaling batten, and 11 is colour developing film, and 12 is detection
Pad, 13 is barrier film.
Detailed description of the invention
Below in conjunction with the accompanying drawings, the present invention is described in detail.
In order to make the purpose of invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, to this
Invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, not
For limiting the present invention.
Embodiment 1
As it is shown in figure 1, a kind of mycotoxin Test paper, including the matrix 7 bottom being placed in, above matrix 7, centre is provided with detection
Pad 12, detecting pad is cup-shaped, and detecting pad 12 side is provided with overflow prevention pad 8, and detecting pad 12 opposite side is provided with leaching sample pad 9, detecting pad 12
Upper end-face edge is all overlying on overflow prevention pad 8 and leaching sample pad 9 upper surface, and overflow prevention pad 8 upper surface is covered with colour developing film 11, soaks sample pad 9 upper surface
It is covered with suction batten 10, inhales batten 10 and be connected with induction zone 5.Colour developing film 11 is in same level with the upper end-face edge of detecting pad 12
On, colour developing film 11 and detecting pad 12 are spaced.Inhale the end face upper end-face edge higher than detecting pad 12 of batten 10, inhale batten 10 and detection
Pad 12 interval.Test paper surface is coated with barrier film 13, and matrix 9 lower surface, the other end of barrier film 13 are fixed in barrier film 13 one end
Batten 10 front end is fixing to be connected with inhaling.Inhale batten 10 surface be evenly coated with agglomerated particles, agglomerated particles with Nanometer Copper as inner core, table
Face is uniformly enclosed with carbonization Bacterial cellulose.Detecting pad 12 surface is evenly coated with aptamers layer.
Aptamers layer is by 37 parts of aptamers of mass parts, 22 parts of gold chlorides, 27 parts of trisodium citrates and 16 parts of fourth sand amine
Alcohol forms, and adjusting aptamers sequence is 5 '-GATCGGGTGTGGGTGGCGTAAAGGGAGCATCGGACAAGAAATGCCACA-
3’.Colour developing film 11 is by 13 parts of nano Au particles of mass parts, 0.7 part of anti-thrombin antibody, 1 part of quartz crystal, 8 parts of hydrosulphonyl silane couplings
Agent and 8 parts of folic acid are made, a diameter of 16nm of nano Au particle.Matrix is by 45 parts of SiO of mass parts2, 12 parts of 4-(4-aminobenzene oxygen
Base) pyridine, 22 parts of polyesteramides and 16 parts of N,N-DMAAs are made.Leaching sample pad and overflow prevention pad are by mass parts 72 part third
Alkene lactic acid, 78 parts of starch-grafted polyacrylonitrile, 3 parts of ZnO and 9 parts of plastic of poly vinyl acetate compositions.Inhale batten by 30 parts of starch
Graft polypropylene nitrile, 47 parts of ethylene glycol dimethacrylates, 16 parts of polymethyl acrylate, 20 parts of methyl methacrylates, 13
Part vinyl acetate and 7 parts of sodium lauryl sulphates are made.
Embodiment 2
The preparation method of agglomerated particles A:
Being immersed in by amycin in the cobalt chloride solution of 4% and take out after 12h, wherein amycin with the ratio of the amount of copper sulfate material is
12:1, puts in the sodium hydroxide solution of nitrogen protection under agitation, and amycin is completely soaked by sodium hydroxide solution,
Ratio cobaltous chloride according to the amount of material: sodium borate: ascorbic acid=1:1:0.7 is slowly added dropwise sodium borate in solution, anti-at 35 DEG C
After answering 30min, in solution, add ascorbic acid, after standing 1 ~ 2h, above-mentioned product deionized water is rinsed for several times, freezing dry
Dry overnight prepare sample.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Any amendment, equivalent and the improvement etc. made within principle, should be included within the scope of the present invention.
Claims (9)
1. a mycotoxin Test paper, it is characterised in that: include the matrix (7) being placed in bottom, in described matrix (7) top
Between to be provided with detecting pad (12) described detecting pad cup-shaped, described detecting pad (12) side is provided with overflow prevention pad (8), described detecting pad
(12) opposite side is provided with leaching sample pad (9), and described detecting pad (12) upper end-face edge is all overlying on overflow prevention pad (8) and the upper table of leaching sample pad (9)
Face, described overflow prevention pad (8) upper surface is covered with colour developing film (11), and described leaching sample pad (9) upper surface is covered with suction batten (10), described suction
Batten (10) is connected with induction zone (5).
2. a kind of mycotoxin Test paper as claimed in claim 1, it is characterised in that: described colour developing film (11) and detecting pad
(12) upper end-face edge is in same level, described colour developing film (11) and detecting pad (12) interval.
3. a kind of mycotoxin Test paper as claimed in claim 1 or 2, it is characterised in that: the top of described suction batten (10)
Face is higher than the upper end-face edge of detecting pad (12), described suction batten (10) and detecting pad (12) interval.
4. a kind of mycotoxin Test paper as claimed in claim 3, it is characterised in that: described Test paper surface is coated with
Barrier film (13), matrix (9) lower surface, the other end of described barrier film (13) and suction batten (10) are fixed in described barrier film (13) one end
Front end is fixing to be connected.
5. a kind of mycotoxin Test paper as claimed in claim 4, it is characterised in that: described suction batten (10) surface is uniform
Scribbling agglomerated particles, described agglomerated particles is with Nanometer Copper as inner core, and surface is uniformly enclosed with carbonization Bacterial cellulose.
6. a kind of mycotoxin Test paper as described in claim 4 or 5, it is characterised in that: described detecting pad (12)
Surface is evenly coated with aptamers layer, and described aptamers layer is by 37 parts of aptamers of mass parts, 22 parts of gold chlorides, 27 parts of citric acids
Trisodium and 16 parts of fourth sand amine alcohol compositions, described accounting aptamers sequence is 5 '-GATCGGGTGTGGGTGGCGTAAAGGGAGCATC
GGACAAGAAATGCCACA-3’。
7. a kind of mycotoxin Test paper as claimed in claim 6, it is characterised in that: described colour developing film (11) is by mass parts
13 parts of nano Au particles, 0.7 part of anti-thrombin antibody, 1 part of quartz crystal, 8 parts of mercaptosilane coupling agents and 8 parts of folic acid are made, institute
State a diameter of 16nm of nano Au particle.
8. a kind of mycotoxin Test paper as claimed in claim 7, it is characterised in that: described matrix is by mass parts 45 parts
SiO2, 12 parts of 4-(4-amino-benzene oxygen) pyridines, 22 parts of polyesteramides and 16 parts of N,N-DMAAs are made.
As claimed in claim 7 or 8 a kind of mycotoxin Test paper, it is characterised in that: described leaching sample pad and overflow prevention pad by
72 parts of propylene lactic acid of mass parts, 78 parts of starch-grafted polyacrylonitrile, 3 parts of ZnO and 9 parts of plastic of poly vinyl acetate compositions.
9. a kind of mycotoxin Test paper as claimed in claim 9, it is characterised in that: described suction batten is connect by 30 parts of starch
Branch polyacrylonitrile, 47 parts of ethylene glycol dimethacrylates, 16 parts of polymethyl acrylate, 20 parts of methyl methacrylates, 13 parts
Vinyl acetate and 7 parts of sodium lauryl sulphates are made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610835146.7A CN106248926A (en) | 2016-09-21 | 2016-09-21 | A kind of mycotoxin Test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610835146.7A CN106248926A (en) | 2016-09-21 | 2016-09-21 | A kind of mycotoxin Test paper |
Publications (1)
| Publication Number | Publication Date |
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| CN106248926A true CN106248926A (en) | 2016-12-21 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610835146.7A Pending CN106248926A (en) | 2016-09-21 | 2016-09-21 | A kind of mycotoxin Test paper |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000017626A1 (en) * | 1998-09-18 | 2000-03-30 | Syntron Bioresearch Inc. | Analytical test device and method of use |
| CN101893523A (en) * | 2009-05-20 | 2010-11-24 | 纳米基因有限公司 | Analyte detecting method and composite |
| CN102121038A (en) * | 2010-12-08 | 2011-07-13 | 天津大学 | Method for preparing cuprous oxide/ bacterial cellulose nano composite material |
| CN102401828A (en) * | 2003-09-23 | 2012-04-04 | 美艾利尔瑞士股份有限公司 | Lateral flow assay devices and methods of use |
| CN102520165A (en) * | 2011-12-29 | 2012-06-27 | 北京康美天鸿生物科技有限公司 | Method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay |
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
| CN104258833A (en) * | 2014-09-24 | 2015-01-07 | 华南师范大学 | Preparation method of novel solid phase microextraction fiber based on nucleic acid aptamer/ nanogold/ porous polymer coating |
-
2016
- 2016-09-21 CN CN201610835146.7A patent/CN106248926A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000017626A1 (en) * | 1998-09-18 | 2000-03-30 | Syntron Bioresearch Inc. | Analytical test device and method of use |
| CN102401828A (en) * | 2003-09-23 | 2012-04-04 | 美艾利尔瑞士股份有限公司 | Lateral flow assay devices and methods of use |
| CN101893523A (en) * | 2009-05-20 | 2010-11-24 | 纳米基因有限公司 | Analyte detecting method and composite |
| CN102121038A (en) * | 2010-12-08 | 2011-07-13 | 天津大学 | Method for preparing cuprous oxide/ bacterial cellulose nano composite material |
| CN102520165A (en) * | 2011-12-29 | 2012-06-27 | 北京康美天鸿生物科技有限公司 | Method for highly sensitive quantitative detection of quantum dot fluorescence immunochromatographic assay |
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
| CN104258833A (en) * | 2014-09-24 | 2015-01-07 | 华南师范大学 | Preparation method of novel solid phase microextraction fiber based on nucleic acid aptamer/ nanogold/ porous polymer coating |
Non-Patent Citations (1)
| Title |
|---|
| 杜兵耀 等: "核酸适配体技术在食品中霉菌毒素检测的研究进展", 《食品科学》 * |
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Application publication date: 20161221 |