CN106350551A - High-moisture-retention trehalose syrup and preparation method thereof - Google Patents
High-moisture-retention trehalose syrup and preparation method thereof Download PDFInfo
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- CN106350551A CN106350551A CN201611026137.XA CN201611026137A CN106350551A CN 106350551 A CN106350551 A CN 106350551A CN 201611026137 A CN201611026137 A CN 201611026137A CN 106350551 A CN106350551 A CN 106350551A
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- trehalose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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Abstract
The invention provides a preparation method of high-moisture-retention trehalose syrup products. The method comprises the following steps: by taking malt syrup as a raw material, carrying out enzymatic conversion, decolorizing and filtering, carrying out ion exchange and concentrating to obtain the finished trehalose syrup products. The method is used to prepare a series of differential trehalose syrup products by taking ordinary malt syrup as a substrate and adjusting the relation between content of malt in the substrate and enzyme adding amount; the method has the advantages of simple production process, short production period and low cost of the products.
Description
Technical field:
The present invention relates to a kind of preparation method of trehalose syrup, belong to starch sugar technical field.
Background technology:
Trehalose has many excellent physicochemical properties such as irreducibility, before food and medicine field has a wide range of applications
Scape, but current trehalose does not also have commonly used in food processing process, main reason is that the price of trehalose is higher,
Promote trehalose produce the final cause of high cost except crystallization outer, also have it isolate and purify yield low, difficult greatly (main because
It is isomerss for maltose and trehalose), mainly removed by expensive separation system of simulated moving bed chromatography,
For the problems referred to above, and Binding experiment room applied research is it has been found that trehalose is also to enter with other syrup in application process
Row is compounding, and the present invention saves compounding process and directly prepares certain purity by adjusting substrate maltose purity and enzyme concentration
Trehalose syrup, take into account the hygroscopicity of maltose and the moisture retention of trehalose.Compared with traditional method, the present invention produces week
Phase is short, low production cost, preparation process is simple.
Content of the invention:
It is an object of the invention to preparing a series of differentiation trehalose syrupy products, in this product, contain glucose, wheat simultaneously
The trehalose syrup of bud sugar, trehalose, maltotriose etc..
The technical scheme is that
A kind of humectant trehalose syrup is it is characterised in that with maltose syrup as raw material, contain glucose, Fructus Hordei Germinatus in product
Sugar, trehalose, maltotriose etc., wherein trehalose accounting (butt percent) are 10-70%.
A kind of humectant trehalose syrup in the present invention, with maltose syrup as raw material, through enzymatic conversion, decolorization filtering,
Ion exchange, concentration obtain finished product Sargassum syrup.
According to above-mentioned steps, used substrate maltose starches maltose purity (accounting for butt) 30-85%
Trehalose synthase enzyme concentration used in enzymatic conversion process is 10-50u/g butt, enzymatic conversion ph4.5-6.0, enzymatic conversion
Temperature 40-60 DEG C.
Beneficial effects of the present invention:
1. this technique preparation process cycle is short, low cost, can promote the market of trehalose to apply significantly；
2. the product that the present invention prepares is different from market existing crystalline trehalose product, fills a hole in the market.
Specific embodiment:
Embodiment 1:
A kind of preparation method of humectant trehalose syrup, comprises the steps: maltose syrup dispensing → enzymatic conversion → decolouring
Filtration → ion exchange → concentration → packaging
With the maltose syrup of maltose content 50%, add a certain proportion of industrial water, adjusting maltose syrup solid content is
30%, it is steam heated to 45 DEG C, add trehalose synthase according to 30u/g substrate contents on dry basis, enzymatic conversion amounts to 40h, through efficient liquid
After the phase chromatograph detection and analysis each constituent content of feed liquid (accounting for contents on dry basis, refer to table 1), feed liquid intensification enzyme denaturing, and add activated carbon
Carry out decolorization filtering, feed liquid after decolouring, enter ion exchange column and carry out desalting processing, and be concentrated into solid content 70%, carry out
Packaging.
Embodiment 2:
A kind of preparation method of humectant trehalose syrup, comprises the steps: maltose syrup dispensing → enzymatic conversion → decolouring
Filtration → ion exchange → concentration → packaging
With the maltose syrup of maltose content 60%, add a certain proportion of industrial water, adjusting maltose syrup solid content is
30%, it is steam heated to 55 DEG C, add trehalose synthase according to 50u/g substrate contents on dry basis, enzymatic conversion amounts to 48h, through efficient liquid
After the phase chromatograph detection and analysis each constituent content of feed liquid (accounting for contents on dry basis, refer to table 1), feed liquid intensification enzyme denaturing, and add activated carbon
Carry out decolorization filtering, feed liquid after decolouring, enter ion exchange column and carry out desalting processing, and be concentrated into solid content 70%, carry out
Packaging.
Embodiment 3:
A kind of preparation method of humectant trehalose syrup, comprises the steps: maltose syrup dispensing → enzymatic conversion → decolouring
Filtration → ion exchange → concentration → packaging
With the maltose syrup of maltose content 70%, add a certain proportion of industrial water, adjusting maltose syrup solid content is
30%, it is steam heated to 60 DEG C, add trehalose synthase according to 10u/g substrate contents on dry basis, enzymatic conversion amounts to 60h, through efficient liquid
After the phase chromatograph detection and analysis each constituent content of feed liquid (account for butt and refer to table 1), feed liquid intensification enzyme denaturing, and add activated carbon row bag
Dress.
Table 1 embodiment feed liquid constituent content
Claims (4)
1. a kind of humectant trehalose syrup is it is characterised in that with maltose syrup as raw material, contain glucose, wheat in product
Bud sugar, trehalose, maltotriose etc., wherein trehalose accounting (butt percent) are 10-70%.
2. a kind of humectant trehalose syrup is it is characterised in that with maltose syrup as raw material, through enzymatic conversion, decolorization filtering,
Ion exchange, concentration obtain finished product Sargassum syrup.
3. according to claim 2 it is characterised in that used maltose syrup maltose purity (accounting for butt) 30-85%.
4. according to claim 2 it is characterised in that the trehalose synthase enzyme concentration that enzymatic conversion is used is done for 10-50u/g
Base, enzymatic conversion ph4.5-6.0, enzymatic conversion temperature 40-60 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611026137.XA CN106350551A (en) | 2016-11-22 | 2016-11-22 | High-moisture-retention trehalose syrup and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611026137.XA CN106350551A (en) | 2016-11-22 | 2016-11-22 | High-moisture-retention trehalose syrup and preparation method thereof |
Publications (1)
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| CN106350551A true CN106350551A (en) | 2017-01-25 |
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| CN201611026137.XA Pending CN106350551A (en) | 2016-11-22 | 2016-11-22 | High-moisture-retention trehalose syrup and preparation method thereof |
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Citations (11)
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|---|---|---|---|---|
| JPH0998779A (en) * | 1995-08-03 | 1997-04-15 | Fuji Seito Kk | Trehalose synthetase, its production and production of trehalose using the enzyme |
| CN1502702A (en) * | 1993-12-09 | 2004-06-09 | 株式会社林原生物化学研究所 | Trehalose-releasing enzyme, and its preparation and use |
| US20080182311A1 (en) * | 2006-07-21 | 2008-07-31 | Academia Sinica | Novel trehalose synthase from Picrophilus torridus and methods of use thereof |
| CN101438782A (en) * | 2008-12-31 | 2009-05-27 | 保龄宝生物股份有限公司 | Moisture-keeping syrup and preparation method thereof |
| CN101831474A (en) * | 2010-04-02 | 2010-09-15 | 山东轻工业学院 | Production method of trehalose |
| CN102154327A (en) * | 2010-12-30 | 2011-08-17 | 广西大学 | Thermomonospora curvata mycose synthetase gene and application thereof |
| CN103409484A (en) * | 2013-08-23 | 2013-11-27 | 山东福田药业有限公司 | Preparation method of superhigh malt syrup |
| CN104531804A (en) * | 2014-12-13 | 2015-04-22 | 保龄宝生物股份有限公司 | Method for production of crystal trehalose and co-production of moisturizing syrup |
| TW201608025A (en) * | 2014-08-22 | 2016-03-01 | 義守大學 | Producing method of trehalose |
| CN106086116A (en) * | 2016-06-30 | 2016-11-09 | 溧阳维信生物科技有限公司 | A kind of trehalose preparation method |
| CN110241152A (en) * | 2018-03-09 | 2019-09-17 | 通辽梅花生物科技有限公司 | The preparation method of compound seaweed sugar product |
-
2016
- 2016-11-22 CN CN201611026137.XA patent/CN106350551A/en active Pending
Patent Citations (11)
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|---|---|---|---|---|
| CN1502702A (en) * | 1993-12-09 | 2004-06-09 | 株式会社林原生物化学研究所 | Trehalose-releasing enzyme, and its preparation and use |
| JPH0998779A (en) * | 1995-08-03 | 1997-04-15 | Fuji Seito Kk | Trehalose synthetase, its production and production of trehalose using the enzyme |
| US20080182311A1 (en) * | 2006-07-21 | 2008-07-31 | Academia Sinica | Novel trehalose synthase from Picrophilus torridus and methods of use thereof |
| CN101438782A (en) * | 2008-12-31 | 2009-05-27 | 保龄宝生物股份有限公司 | Moisture-keeping syrup and preparation method thereof |
| CN101831474A (en) * | 2010-04-02 | 2010-09-15 | 山东轻工业学院 | Production method of trehalose |
| CN102154327A (en) * | 2010-12-30 | 2011-08-17 | 广西大学 | Thermomonospora curvata mycose synthetase gene and application thereof |
| CN103409484A (en) * | 2013-08-23 | 2013-11-27 | 山东福田药业有限公司 | Preparation method of superhigh malt syrup |
| TW201608025A (en) * | 2014-08-22 | 2016-03-01 | 義守大學 | Producing method of trehalose |
| CN104531804A (en) * | 2014-12-13 | 2015-04-22 | 保龄宝生物股份有限公司 | Method for production of crystal trehalose and co-production of moisturizing syrup |
| CN106086116A (en) * | 2016-06-30 | 2016-11-09 | 溧阳维信生物科技有限公司 | A kind of trehalose preparation method |
| CN110241152A (en) * | 2018-03-09 | 2019-09-17 | 通辽梅花生物科技有限公司 | The preparation method of compound seaweed sugar product |
Non-Patent Citations (3)
| Title |
|---|
| K. SAITO等: "Production of trehalose synthase from a basidiomycete, Grifola frondosa, in Escherichia coli", 《APPL MICROBIOL BIOTECHNOL》 * |
| 刘建龙等: "麦芽糖生物合成海藻糖的途径及合成条件的研究", 《食品工业科技》 * |
| 田淵彰彦等: "新規酵二素による澱粉からのトレハロース生成", 《応用糖質科学》 * |
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Application publication date: 20170125 |