CN106349305A - Extraction and preparation method of streptomycin sulfate - Google Patents

Extraction and preparation method of streptomycin sulfate Download PDF

Info

Publication number
CN106349305A
CN106349305A CN201610732913.1A CN201610732913A CN106349305A CN 106349305 A CN106349305 A CN 106349305A CN 201610732913 A CN201610732913 A CN 201610732913A CN 106349305 A CN106349305 A CN 106349305A
Authority
CN
China
Prior art keywords
streptomycin
streptomycin sulfate
preparation
described step
molecule polymer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201610732913.1A
Other languages
Chinese (zh)
Inventor
张健
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HEBEI SHENGXUE DACHENG PHARMACEUTICAL CO Ltd
Original Assignee
HEBEI SHENGXUE DACHENG PHARMACEUTICAL CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HEBEI SHENGXUE DACHENG PHARMACEUTICAL CO Ltd filed Critical HEBEI SHENGXUE DACHENG PHARMACEUTICAL CO Ltd
Priority to CN201610732913.1A priority Critical patent/CN106349305A/en
Publication of CN106349305A publication Critical patent/CN106349305A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20Carbocyclic rings
    • C07H15/22Cyclohexane rings, substituted by nitrogen atoms
    • C07H15/238Cyclohexane rings substituted by two guanidine radicals, e.g. streptomycins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The invention discloses an extraction and preparation method of streptomycin sulfate. The extraction and preparation method comprises the steps of acidizing, filtering, adsorbing, pre-washing, eluting, decompression concentrating, salt forming, decoloring, concentrating and spray-drying, thus obtaining a finished product. The extraction and preparation method of the streptomycin sulfate has the beneficial effects that the technical problems in the prior art that the extraction and preparation route is long, the production efficiency is low, the investment cost is high, the yield is low, the product color is poor, and the qualification rate is low are solved and has the advantages that the extraction and preparation process is short, the procedures are simple, the production efficiency is high, the investment cost is low, the consumption of active carbon is small, the yield is high, the product color is good, and the qualification rate is high.

Description

A kind of streptomycin sulfate extracts preparation method
Technical field
The invention belongs to extract drugs field, it is related to a kind of extraction preparation method of pharmaceutical raw material, more particularly to one kind Streptomycin sulfate extracts preparation method.
Background technology
Streptomycin sulfate is a kind of aminoglycoside antibioticss.This product has powerful antibacterial action to tubercule bacillus, and it is minimum Mlc (mic) is generally 0.5mg/l.It is to many gram negative bacterias (g-) such as escherichia coli, pneumobacilluses, enterobacteria Genus, Salmonella, Brucella etc. also have an antibacterial action, poor to gram positive bacteria (g+) antibacterial activity.It is for treatment Various tuberculosis have significant curative effect, are class drug of first choices for the treatment of pulmonary tuberculosis, are additionally operable to treat the plague and Gram-negative Microbial urinary tract infection, intestinal infection, tuberculous meningitis, septicemia, pneumonia, peritonitis etc..
At present, the method extracting preparation is the fermentation liquid acidifying of streptomycin, by ceramic membrane filter, sodium form faintly acid sun from Sub-exchange resin dynamic adsorption, aqueous sulfuric acid desorbing, one time stripping liquid adsorbs through macropore primary amine resins again, aqueous sulfuric acid Desorbing, secondary stripping liquid carries out desalination neutralization through the mixed bed that resin forms, and then uses activated carbon decolorizing, thin film concentration, ultrafiltration Remove endotoxin and pigment, be spray-dried and obtain streptomycin sulfate finished product.
In actual production operating process, this extraction preparation method there are following weak point:
Extract preparation process through ion exchange twice, circuit is longer, low production efficiency, high cost, and yield is low;
The streptomycin sulfate finished product color level that extraction prepares is poor, and conforming product rate is low.
Content of the invention
The present invention is to solve problem of the prior art, provides a kind of production efficiency high, input cost is low, eluting liquid hold-up High, clarity is good, and activated carbon dosage is little, high income, and product color level is good, and the new side of streptomycin sulfate is prepared in the high extraction of qualification rate Method.
The present invention is to realize its purpose to the technical scheme is that a kind of streptomycin sulfate extracts preparation method, the method Comprise the steps:
A, filtration: streptomycin sulfate fermentation liquid is carried out being acidified to ph2.0 ~ 4.0, then carries out ceramic membrane filter, obtain filtrate;
B, absorption: with alkali, the filtrate ph that step a obtains is adjusted to 6.0 ~ 8.0, enters action with streptomycin imprint molecule polymer State is adsorbed;
C, prewashing: prewashing is carried out to the streptomycin imprint molecule polymer in step b with soft water;
D, eluting: with methanol aqueous solution or ethanol water, eluting is carried out to the streptomycin imprint molecule polymer in step c, obtain To eluent;
E, concentrating under reduced pressure: vacuum distillation is carried out to eluent, obtains concentrated solution;
F, one-tenth salt: concentrated solution addition sulphuric acid is become salt, obtains into saline solution;
G, decolouring: saline solution will be become to add activated carbon, filter, obtain destaining solution;
H, concentration: with NF membrane, destaining solution is concentrated, obtain finished fluid;
I, spray drying: finished fluid is spray-dried, gets product.
Further, the acid being acidified in described step a is oxalic acid, and the alkali in step c is sodium hydroxide.
Further, the flow velocity of the dynamic adsorption in described step c is 0.5 ~ 2.0bv/h, and streptomycin imprint molecule is polymerized The adsorbance of thing is 100 ~ 150g/l.
Further, the prewashing flow velocity in described step c is 1.0 ~ 2.0bv/h, and the wherein consumption of soft water prints for streptomycin 1.0 ~ 4.0 times of sub- polymer volume of scoring.
Further, in described step d, elution flow rate is 0.1-1.0bv/h, the body of methanol and water in methanol aqueous solution Long-pending ratio is 0.5 ~ 4.0:1, and in ethanol water, ethanol and the volume ratio of water are 0.5 ~ 4.0:1.
Further, in described step e, the temperature of vacuum distillation is 30 ~ 40 DEG C.
Further, adding sulphuric acid to adjust ph in described step f is 4.5 ~ 6.0.
Further, in described step f, the molecular weight of sodium filter membrane is 200 ~ 500da.
Have the beneficial effects that using produced by technique scheme: the invention reside in by improving original sulphuric acid strepto- Plain extraction and preparation technique, obtains meeting the streptomycin sulfate of usp and cp standard, by using streptomycin imprint molecule polymer pair Streptomycin in fermentation liquid carries out being enriched with, purification, removes most albumen, pigment and other impurities, in the eluent obtaining Content of streptomycin is high, changes existing ion exchange extraction preparation production technology, improves production efficiency, reduce and put into into This, activated carbon dosage is little, high income, and product color level is good, and qualification rate is high.Streptomycin sulfate total recovery more than 95%, higher than mesh The level of front yield 80% about.
Specific embodiment
Below the embodiment it is clear that described is clearly and completely described to the technical scheme in the embodiment of the present invention It is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common The every other embodiment that technical staff is obtained under the premise of not making creative work, broadly falls into the model of present invention protection Enclose.
Embodiment 1
A, filtration: by streptomycin fermentation liquid 60l, potency 28550u/ml, product weight 1.713 kg.Adjust ph with oxalic acid to arrive 2.0, then filtered through ceramic membrane, obtained filtrate;
B, absorption: with sodium hydroxide, filtrate ph is adjusted to 6.0, then with streptomycin imprint molecule polymer, action is entered to filtrate State is adsorbed, and is squeezed into peristaltic pump in the pillar installing streptomycin imprint molecule polymer, and flow velocity is 0.5bv/h, strepto- Plain imprint molecule Polymer adsorption amount is 100g/l;
C, prewashing: with soft water, prewashing is carried out to the streptomycin imprint molecule polymer after adsorbing in step b, flow velocity is 1.0bv/h, The consumption of soft water is 1.0 times of streptomycin imprint molecule polymer volume;
D, eluting: with methanol aqueous solution, the streptomycin imprint molecule polymer after prewashing is carried out with eluting, the volume of methanol and water For 0.5:1, flow velocity is 0.1bv/h to ratio, obtains eluent;
E, concentrating under reduced pressure: eluent is added in vacuum distillation apparatus and is concentrated, temperature is 30 DEG C, obtains concentrated solution;
F, one-tenth salt: adding sulphuric acid to adjust ph in concentrated solution is 4.5, obtains into saline solution;
G, decolouring: to becoming to add the stirring decolouring of 200g activated carbon in saline solution, filter, obtain destaining solution;
H, concentration: with the NF membrane that molecular weight is 300da, destaining solution is concentrated, obtain finished fluid;
I, spray drying: finished fluid is spray-dried, obtains the streptomycin sulfate finished product 1.88kg meeting cp and usp standard, receive Rate 95.4%.
Embodiment 2
A, filtration: by streptomycin fermentation liquid 50l, potency 28000u/ml, product weight 1.4 kg.Adjust ph to 4.0 with oxalic acid, Then filtered through ceramic membrane, obtained filtrate;
B, absorption: with sodium hydroxide, filtrate ph is adjusted to 7.2, then with streptomycin imprint molecule polymer, action is entered to filtrate State is adsorbed, and is squeezed into peristaltic pump in the pillar installing streptomycin imprint molecule polymer, and flow velocity is 2.0bv/h, strepto- Plain imprint molecule Polymer adsorption amount is 150g/l;
C, prewashing: with soft water, prewashing is carried out to the streptomycin imprint molecule polymer after adsorbing in step b, flow velocity is 1.0bv/h, The consumption of soft water is 4.0 times of streptomycin imprint molecule polymer volume;
D, eluting: with methanol aqueous solution, the streptomycin imprint molecule polymer after prewashing is carried out with eluting, the volume of methanol and water For 4.0:1, flow velocity is 1.0bv/h to ratio, obtains eluent;
E, concentrating under reduced pressure: eluent is added in vacuum distillation apparatus and is concentrated, temperature is 40 DEG C, obtains concentrated solution;
F, one-tenth salt: adding sulphuric acid to adjust ph in concentrated solution is 6.0, obtains into saline solution;
G, decolouring: to becoming to add the stirring decolouring of 210g activated carbon in saline solution, filter, obtain destaining solution;
H, concentration: with the NF membrane that molecular weight is 500da, destaining solution is concentrated, obtain finished fluid;
I, spray drying: finished fluid is spray-dried, obtains the streptomycin sulfate finished product 1.61kg meeting cp and usp standard, receive Rate 96%.
Embodiment 3
A, filtration: by streptomycin fermentation liquid 52l, potency 28600u/ml, product weight 1.487 kg.Adjust ph with oxalic acid to arrive 2.0, then filtered through ceramic membrane, obtained filtrate;
B, absorption: with sodium hydroxide, filtrate ph is adjusted to 6.8, then with streptomycin imprint molecule polymer, action is entered to filtrate State is adsorbed, and is squeezed into peristaltic pump in the pillar installing streptomycin imprint molecule polymer, and flow velocity is 1.25bv/h, strepto- Plain imprint molecule Polymer adsorption amount is 125g/l;
C, prewashing: with soft water, prewashing is carried out to the streptomycin imprint molecule polymer after adsorbing in step b, flow velocity is 1.5bv/h, The consumption of soft water is 2.5 times of streptomycin imprint molecule polymer volume;
D, eluting: with methanol aqueous solution, the streptomycin imprint molecule polymer after prewashing is carried out with eluting, the volume of methanol and water For 2.25:1, flow velocity is 0.55bv/h to ratio, obtains eluent;
E, concentrating under reduced pressure: eluent is added in vacuum distillation apparatus and is concentrated, temperature is 35 DEG C, obtains concentrated solution;
F, one-tenth salt: adding sulphuric acid to adjust ph in concentrated solution is 5.25, obtains into saline solution;
G, decolouring: to becoming to add the stirring decolouring of 205g activated carbon in saline solution, filter, obtain destaining solution;
H, concentration: with the NF membrane that molecular weight is 400da, destaining solution is concentrated, obtain finished fluid;
I, spray drying: finished fluid is spray-dried, obtains the streptomycin sulfate finished product 1.74kg meeting cp and usp standard, receive Rate 96.6%.
Embodiment 4
A, filtration: by streptomycin fermentation liquid 46l, potency 29000u/ml, product weight 1.334 kg.Adjust ph with oxalic acid to arrive 3.1, then filtered through ceramic membrane, obtained filtrate;
B, absorption: with sodium hydroxide, filtrate ph is adjusted to 7.5, then with streptomycin imprint molecule polymer, action is entered to filtrate State is adsorbed, and is squeezed into peristaltic pump in the pillar installing streptomycin imprint molecule polymer, and flow velocity is 0.9bv/h, strepto- Plain imprint molecule Polymer adsorption amount is 110g/l;
C, prewashing: with soft water, prewashing is carried out to the streptomycin imprint molecule polymer after adsorbing in step b, flow velocity is 1.2bv/h, The consumption of soft water is 1.5 times of streptomycin imprint molecule polymer volume;
D, eluting: with methanol aqueous solution, the streptomycin imprint molecule polymer after prewashing is carried out with eluting, the volume of methanol and water For 3.2:1, flow velocity is 0.3bv/h to ratio, obtains eluent;
E, concentrating under reduced pressure: eluent is added in vacuum distillation apparatus and is concentrated, temperature is 36 DEG C, obtains concentrated solution;
F, one-tenth salt: adding sulphuric acid to adjust ph in concentrated solution is 5.7, obtains into saline solution;
G, decolouring: to becoming to add the stirring decolouring of 250g activated carbon in saline solution, filter, obtain destaining solution;
H, concentration: with the NF membrane that molecular weight is 300da, destaining solution is concentrated, obtain finished fluid;
I, spray drying: finished fluid is spray-dried, obtains the streptomycin sulfate finished product 1.6kg meeting cp and usp standard, yield 96.4%.
Embodiment 5
A, filtration: by streptomycin fermentation liquid 65l, potency 28930u/ml, product weight 1.88 kg.Adjust ph to 2.6 with oxalic acid, Then filtered through ceramic membrane, obtained filtrate;
B, absorption: with sodium hydroxide, filtrate ph is adjusted to 6.4, then with streptomycin imprint molecule polymer, action is entered to filtrate State is adsorbed, and is squeezed into peristaltic pump in the pillar installing streptomycin imprint molecule polymer, and flow velocity is 1.8bv/h, strepto- Plain imprint molecule Polymer adsorption amount is 144g/l;
C, prewashing: with soft water, prewashing is carried out to the streptomycin imprint molecule polymer after adsorbing in step b, flow velocity is 1.7bv/h, The consumption of soft water is 2.3 times of streptomycin imprint molecule polymer volume;
D, eluting: with methanol aqueous solution, the streptomycin imprint molecule polymer after prewashing is carried out with eluting, the volume of methanol and water For 3.3:1, flow velocity is 0.6bv/h to ratio, obtains eluent;
E, concentrating under reduced pressure: eluent is added in vacuum distillation apparatus and is concentrated, temperature is 34 DEG C, obtains concentrated solution;
F, one-tenth salt: adding sulphuric acid to adjust ph in concentrated solution is 5.0, obtains into saline solution;
G, decolouring: to becoming to add the stirring decolouring of 220g activated carbon in saline solution, filter, obtain destaining solution;
H, concentration: with the NF membrane that molecular weight is 420da, destaining solution is concentrated, obtain finished fluid;
I, spray drying: finished fluid is spray-dried, obtains the streptomycin sulfate finished product 2.2kg meeting cp and usp standard, yield 96.2%.

Claims (8)

1. a kind of streptomycin sulfate extract preparation method it is characterised in that: the method comprises the steps:
A, filtration: streptomycin sulfate fermentation liquid is carried out being acidified to ph2.0 ~ 4.0, then carries out ceramic membrane filter, obtain filtrate;
B, absorption: with alkali, the filtrate ph that step a obtains is adjusted to 6.0 ~ 8.0, enters action with streptomycin imprint molecule polymer State is adsorbed;
C, prewashing: prewashing is carried out to the streptomycin imprint molecule polymer after absorption with soft water;
D, eluting: the streptomycin imprint molecule polymer after prewashing in step c is carried out with methanol aqueous solution or ethanol water Eluting, obtains eluent;
E, concentrating under reduced pressure: vacuum distillation is carried out to eluent, obtains concentrated solution;
F, one-tenth salt: concentrated solution addition sulphuric acid is become salt, obtains into saline solution;
G, decolouring: saline solution will be become to add activated carbon, filter, obtain destaining solution;
H, concentration: with NF membrane, destaining solution is concentrated, obtain finished fluid;
I, spray drying: finished fluid is spray-dried, gets product.
2. a kind of streptomycin sulfate according to claim 1 extracts preparation method it is characterised in that acid in described step a The acid changed is oxalic acid, and the alkali in step c is sodium hydroxide.
3. a kind of streptomycin sulfate according to claim 1 extracts preparation method it is characterised in that in described step c The flow velocity of dynamic adsorption is 0.5 ~ 2.0bv/h, and the adsorbance of streptomycin imprint molecule polymer is 100 ~ 150g/l.
4. a kind of streptomycin sulfate according to claim 1 extracts preparation method it is characterised in that in described step c Prewashing flow velocity is 1.0 ~ 2.0bv/h, and the wherein consumption of soft water is 1.0 ~ 4.0 times of streptomycin imprint molecule polymer volume.
5. a kind of streptomycin sulfate according to claim 1 extracts preparation method it is characterised in that in described step d Elution flow rate is 0.1 ~ 1.0bv/h, and in methanol aqueous solution, methanol and the volume ratio of water are 0.5 ~ 4.0:1, second in ethanol water Alcohol is 0.5 ~ 4.0:1 with the volume ratio of water.
6. a kind of streptomycin sulfate according to claim 1 extracts preparation method it is characterised in that in described step e The temperature of vacuum distillation is 30 ~ 40 DEG C.
7. a kind of streptomycin sulfate according to claim 1 extracts preparation method it is characterised in that in described step f Adding sulphuric acid to adjust ph is 4.5 ~ 6.0.
8. a kind of streptomycin sulfate according to claim 1 extracts preparation method it is characterised in that in described step f The molecular weight of sodium filter membrane is 200 ~ 500da.
CN201610732913.1A 2016-08-27 2016-08-27 Extraction and preparation method of streptomycin sulfate Withdrawn CN106349305A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610732913.1A CN106349305A (en) 2016-08-27 2016-08-27 Extraction and preparation method of streptomycin sulfate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610732913.1A CN106349305A (en) 2016-08-27 2016-08-27 Extraction and preparation method of streptomycin sulfate

Publications (1)

Publication Number Publication Date
CN106349305A true CN106349305A (en) 2017-01-25

Family

ID=57854292

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610732913.1A Withdrawn CN106349305A (en) 2016-08-27 2016-08-27 Extraction and preparation method of streptomycin sulfate

Country Status (1)

Country Link
CN (1) CN106349305A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110357934A (en) * 2019-07-31 2019-10-22 陕西麦可罗生物科技有限公司 A kind of process reducing kasugarnycin production process sewage quantity
CN117259383A (en) * 2023-10-30 2023-12-22 河南理工大学 Organic solid waste treatment process

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1632563A (en) * 2005-01-06 2005-06-29 湖南纽尔科技有限公司 Streptomycin molecular engram solid phase extraction column and preparing process and application thereof
CN1667409A (en) * 2005-01-06 2005-09-14 湖南纽尔科技有限公司 Molecular engram integrally separating column for gentamycin, streptomycin and neomycin and preparing process thereof
CN101139411A (en) * 2006-09-06 2008-03-12 天津科技大学 Preparation of molecule marking polymer capable of removing penicillins antibiotics in fresh milk
CN103739643A (en) * 2013-12-30 2014-04-23 华东理工大学 Recovering, separating and purifying method of erythrocin by means of molecular imprinting technique
CN104610395A (en) * 2015-01-19 2015-05-13 河北圣雪大成制药有限责任公司 Method for extracting neomycin sulfate from neomycin sulfate fermentation broth
CN105524130A (en) * 2015-12-21 2016-04-27 河北圣雪大成制药有限责任公司 Extraction method of streptomycin sulfate

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1632563A (en) * 2005-01-06 2005-06-29 湖南纽尔科技有限公司 Streptomycin molecular engram solid phase extraction column and preparing process and application thereof
CN1667409A (en) * 2005-01-06 2005-09-14 湖南纽尔科技有限公司 Molecular engram integrally separating column for gentamycin, streptomycin and neomycin and preparing process thereof
CN101139411A (en) * 2006-09-06 2008-03-12 天津科技大学 Preparation of molecule marking polymer capable of removing penicillins antibiotics in fresh milk
CN103739643A (en) * 2013-12-30 2014-04-23 华东理工大学 Recovering, separating and purifying method of erythrocin by means of molecular imprinting technique
CN104610395A (en) * 2015-01-19 2015-05-13 河北圣雪大成制药有限责任公司 Method for extracting neomycin sulfate from neomycin sulfate fermentation broth
CN105524130A (en) * 2015-12-21 2016-04-27 河北圣雪大成制药有限责任公司 Extraction method of streptomycin sulfate

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
任杰,等: "分子印记技术研究进展", 《生命的化学》 *
郭宇姝,等: "分子印记聚合物技术在固相萃取中的应用及影响因素", 《国外医学药学分册》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110357934A (en) * 2019-07-31 2019-10-22 陕西麦可罗生物科技有限公司 A kind of process reducing kasugarnycin production process sewage quantity
CN117259383A (en) * 2023-10-30 2023-12-22 河南理工大学 Organic solid waste treatment process
CN117259383B (en) * 2023-10-30 2024-05-17 河南理工大学 Organic solid waste treatment process

Similar Documents

Publication Publication Date Title
CN106818752B (en) A kind of preparation method of high-content kasugarnycin aqua
CN104610395B (en) A method of extracting neomycinsulphate from neomycinsulphate zymotic fluid
CN105524130B (en) A kind of streptomycin sulphate extracting method
CN106866759A (en) The technique that grosvenor momordica flavonoid is produced from the waste liquid of Momordica-Glycosides decolorizing resin post discharge
CN102875669B (en) Method for separating and extracting ovotransferrin
CN103087126A (en) Preparation method of zhongshengmycin bulk drug
CN104693250B (en) Method for purifying acarbose from acarbose-containing solution
CN106349305A (en) Extraction and preparation method of streptomycin sulfate
CN105566458A (en) Method for preparing bacitracin and method for preparing zinc salt of bacitracin
CN110467302B (en) Method for preparing edible salt by using deep sea water
CN105274182A (en) Process for efficiently extracting L-valine from fermentation liquor
CN106083952A (en) A kind of method extracting gentamycin sulfate from gentamicin fermentation broth
CN106928288B (en) A kind of preparation method of dihydrostreptomycin sulfate
CN107586820B (en) Method for producing momordica grosvenori protein from mogroside extraction waste liquid of momordica grosvenori
CN104119229A (en) Technology for producing pure chlorogenic acid
CN108329363A (en) The minimizing technology of quinhydrones in alpha-arbutin conversion fluid
CN102229540B (en) Method for producing lysine acetate for injection
CN103450295A (en) Method for purifying gentamicin sulphate
CN102633848B (en) Method for removing impurities from gentamicin
CN103159643B (en) Technology for whole membrane extraction of L-glutamine fermentation broth
CN111116768A (en) Method for recovering baicalein from baicalin production waste liquid
CN111377898A (en) Preparation process of cranberry extract procyanidine
CN104327167A (en) Technology for extracting polymyxin B through precipitation method
CN111635447B (en) Method for extracting high-purity conjugated bile acid from oxgall
CN114262297A (en) Preparation method of ergothioneine

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20170125