CN103450295A - Method for purifying gentamicin sulphate - Google Patents
Method for purifying gentamicin sulphate Download PDFInfo
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Abstract
The invention discloses a method for purifying gentamicin sulphate. The method comprises the steps of: carrying out static exchange on a gentamicin fermentation broth by using 732 cation resin; washing by diluted hydrochloric acid; carrying out primary washing by dilute ammonia, primary elution by thick ammonia and primary concentration; and carrying out the technical processes such as pH adjustment, resin fixed bed dynamic adsorption, secondary washing by dilute ammonia, resolution, secondary concentration, salt conversion decoloration, and spraying to produce the gentamicin sulphate. According to the method, the quality indexes of the gentamicin sulphate bulk drug product can reach the requirements of Chinese Pharmacopoeia 2010, and the content of each related substance can conform to the quality requirements of Chinese Pharmacopoeia 2010 on bulk drugs for production of small-volume injection of gentamicin sulphate. The method has the characteristics of high yield, good quality and low cost. Meanwhile, original production equipment is combined in production, so that low investment cost is realized and the maximum economic benefit is achieved.
Description
Technical field
The invention belongs to pharmaceutical chemistry technical field, particularly relate to a kind of method of gentamicin sulphate purifying.
Background technology
According to the quality of production situation of each manufacturing enterprise of current national gentamicin sulphate bulk drug, with regard to its C
1the indivedual manufacturing enterprises of the index of component all are difficult to effectively reach the former " requirement of the component of 2005 editions gentamicin sulphate quality standards of Chinese pharmacopoeia.And " Chinese pharmacopoeia 2010 editions is in gentamicin sulphate quality formulation standard, except 2005 editions require, the content of its related substance (sisomicin, micronomicin, single impurity and total impurities) has also been included standard in.Due to the bulk drug of the gentamicin sulphate in standards of pharmacopoeia and the small-volume injection consistence in component and its related substances, the quality of gentamicin sulphate small-volume injection is reached " 2010 editions standards of formulating of Chinese pharmacopoeia, conversely, just must continue to improve the quality index of gentamicin sulphate bulk drug, the content of the related substance of the raw materials used medicine of small-volume injection must continue to reduce.Only make that sisomicin must not cross 1.1%, micronomicin must not cross 1.6%, single impurity must not be crossed 1.1%, always must not mix 2.6%, just can make related substance in the finished product of small-volume injection as sisomicin must not cross 2.0%, micronomicin must not cross 3.0%, single impurity must not cross 2.0%, total impurities must not be crossed 5.0% etc. and reach 2010 editions standards of being formulated of pharmacopeia." Chinese pharmacopoeia (2010 editions) becomes the highest standard reason in the world to gentamicin to new edition that Here it is.The importantly enforcement of this standard, will make each manufacturer of gentamicin sulphate bulk drug, must improve in time the quality of product, carrys out the demand of satisfying the market.Otherwise will face the threat that stops production or change the line of production or close down.
Although each enterprise is in mutagenesis and the seed selection of bacterial classification, comprise quil small single-cell bacteria, magneta colour small single-cell bacteria and olive star small single-cell bacteria, the adjustment of fermentating formula, and the regulation and control aspect of fermented extracted aspect processing parameter, a large amount of research and cut-and-try work have all been done, consequently all be difficult to reach " standard that Chinese pharmacopoeia is 2010 editions, although or reached standard, the strange height of production cost caused.
Summary of the invention
Present situation for above-mentioned production gentamicin sulphate quality, purpose of the present invention just is to provide a kind of method of gentamicin sulphate purifying, to realize the meeting bulk drug of producing gentamicin sulphate small-volume injection quality standard, make it reach the reasonableness of technique and the economy of production.
For achieving the above object, the method of gentamicin sulphate purifying of the present invention is through 732 resin cation (R.C.) static exchange by the gentamicin sulphate fermented liquid, dilute hydrochloric acid, rare ammonia stripping once, dense ammonia wash-out once, once concentration, through adjusting pH, resin fixed bed dynamic adsorption, the rare ammonia stripping of secondary, wash-out, secondary concentration, turning the salt decolouring, the processing steps such as spraying are realized again.
The method of gentamicin sulphate purifying of the present invention specifically comprises the following steps:
1., fermented liquid is pressed into or pumps in the resins exchange storage tank, according to its volume and estimation unit, by the saturation ratio of single 60000 ~ 90000u/ml, add 732 type resin cation (R.C.)s, in the situation that stir, exchange more than 6 hours, then separation resin, deironing, and rinsing is clean, obtains saturated resin;
2., by the suction of above-mentioned saturated resin sun post, pass into positive post with the washings of 0.30 ~ 0.50mol/L hydrochloric acid and washed, flow is 1.0 ~ 2.0BV, to elutant till checking without calcium, magnesium ion;
3., by the saturated resin after above-mentioned pickling, by purified water, rinse, to go out liquid without chlorion after, the rarer ammonia that passes into 0.09 ~ 0.11mol/L carries out once rare ammonia stripping, flow is 1.0 ~ 2.0BV, till washing out and occurring after the unit of peak; And in time series connection with the purified water square impact to elutant through check the 711 type resin anion(R.A) posts without chlorion, simultaneously, the strong aqua of 3.0 ~ 3.2mol/L is passed into to positive post wash-out, cloudy post decolouring, flow is 0.4 ~ 1.0BV, and collects elutriant, the tail sample is controlled at below 1000u/ml and stops wash-out;
4., above-mentioned elutriant is carried out under vacuum degree condition to thin film concentration or nanofiltration, till making concentrated unit reach 8 ~ 120,000 u/ml; Obtain once concentration liquid;
5., by above-mentioned once concentration liquid under agitation, slowly add industrial sulphuric acid or hydrochloric acid to be adjusted pH7.0 ~ 7.8, obtain the gentamicin neutral solution;
6. above-mentioned gentamicin neutral solution is pressed into or pumps into the fixed bed be equipped with through slightly acidic macroporous resin or SP series or the fine serial macroreticular resin of polypropylene and dynamically adsorbed, flow is 0.2 ~ 1.5BV, to adsorbing saturated stopping.And collect it and gone here and there post absorption containing waste liquid of effective composition.Adsorb saturated after, then replaced adsorbing saturated pillar by purified water, its waste liquid continues the absorption of string post;
7. by above-mentioned absorption, saturated resin column carries out the rare ammonia stripping of secondary, and the rare ammonia stripping of secondary is the rare ammonia stripping of gradient.Wherein, rare ammonia concentration of gradient 1 is 0.1N, and flow is 0.5 ~ 2.0BV, till washing out waste liquid and occurring after the unit of peak; Rare ammonia concentration of gradient 2 is 0.15 ~ 0.2N, and flow is 0.5 ~ 2.0BV, till washing out waste liquid and occurring after the unit of peak; Rare ammonia concentration of gradient 3 is 0.2 ~ 0.3N, and flow is 0.5 ~ 2.0BV, till washing out waste liquid and occurring after the unit of peak; Purified water washing is near neutral or without the ammonia flavor again, and looks into the pillar outlet and stop without acid ion;
8. above-mentioned resin column after the rare ammonia stripping of secondary is resolved, desorbed solution adopts the aqueous solution preparation of ethanol or the Virahol of 5-10%, and flow is 0.2 ~ 1.0BV, and resolving to the tail sample is that 837ug/ml stops.Obtain desorbed solution;
9. above-mentioned desorbed solution is carried out under vacuum degree condition to thin film concentration or nanofiltration, till making concentrated unit reach 18 ~ 320,000 u/ml, obtain secondary concentration liquid;
10. by above-mentioned secondary concentration liquid under agitation, slowly add 6.0mol/L analytical pure sulfuric acid liquid to be turned salt, control pH 5.8 ~ 6.8, obtain gentamicin sulfate solution; Add the medical needle shaped activated carbon that weight ratio is 1:0.1-0.3 in gentamicin sulfate solution, use the steam indirect heating, be warming up to 65-70 ℃ in 20-30min, and decoloured under the condition of insulation 45min, and, after its intracellular toxin is qualified after testing, then binder filters, obtain the de-liquid of charcoal;
According to production method provided by the invention, not only can make gentamicin sulphate bulk drug product quality indicator reach the " requirement that Chinese pharmacopoeia is 2010 editions, and " 2010 editions gentamicin sulphate low capacity small-volume injections of Chinese pharmacopoeia are produced the specification of quality of raw materials used medicine can to make the content of each related substance meet.Have yield high, quality is good, the characteristics that cost is low., and produced in conjunction with the original production equipment simultaneously, reached less investment, to realize maximum economic benefit.
Embodiment
The method of gentamicin sulphate purifying of the present invention is that gentamicin fermentation broth is through 732 resin cation (R.C.) static exchange, dilute hydrochloric acid washing, rare ammonia stripping once, dense ammonia wash-out once, once concentration, through adjusting pH, resin fixed bed dynamic adsorption, the rare ammonia stripping of secondary, parsing, secondary concentration, turning the salt decolouring, the processing steps such as spraying are produced gentamicin sulphate again.
For this reason, the method for gentamicin sulphate purifying of the present invention specifically comprises the following steps:
1., fermented liquid is pressed into or pumps in the resins exchange storage tank, according to its volume and estimation unit, the 732 type resin cation (R.C.)s of throwing by the saturation ratio of single 70000u/ml, in the situation that stir, exchange more than 6 hours, then separation resin, deironing, and rinsing is clean, obtains saturated resin.
2., by the suction of above-mentioned saturated resin sun post, pass into positive post with the washings of 0.35mol/L hydrochloric acid and washed, flow is 1.5BV, to elutant till checking without calcium, magnesium ion.
3., by the saturated resin after above-mentioned pickling, by purified water, rinse, to go out liquid without chlorion after, the rarer ammonia that passes into 0.1mol/L carries out once rare ammonia stripping, flow is 1.5 BV, till washing out and occurring after the unit of peak; And in time series connection with the purified water square impact to elutant through check the 711 type resin anion(R.A) posts without chlorion, simultaneously, the strong aqua of 3.1mol/L is passed into to positive post and carries out wash-out, flow is 0.5BV, and collects elutriant.
4., above-mentioned elutriant is carried out under vacuum degree condition to thin film concentration or nanofiltration, till making concentrated unit reach 8.8 ten thousand u/ml; Obtain once concentration liquid.
5., by above-mentioned once concentration liquid under agitation, slowly add industrial sulphuric acid to be adjusted pH7.4, obtain the gentamicin neutral solution.
6. above-mentioned gentamicin neutral solution is pressed into or pumps into the fixed bed be equipped with through SP1900 series macroporous resin and carry out dynamic adsorption, flow is 1.0BV, to adsorbing saturated stopping.And collect containing the waste liquid of effective composition and gone here and there post absorption.By purified water, to adsorbing saturated pillar, replaced, its waste liquid continues the absorption of string post again.
7. by above-mentioned absorption, saturated resin column carries out the rare ammonia stripping of secondary, and the rare ammonia stripping of secondary is the rare ammonia stripping of gradient.Wherein, rare ammonia concentration of gradient 1 is 0.1N, and flow is 0.5BV, till washing out waste liquid and occurring after the unit of peak; Rare ammonia concentration of gradient 2 is 0.18N, and flow is 0.8BV, till washing out waste liquid and occurring after the unit of peak; Rare ammonia concentration of gradient 3 is 0.25N, and flow is 1.2BV, till washing out waste liquid and occurring after the unit of peak; Purified water washing is near neutral or without the ammonia flavor again, and looks into the pillar outlet and stop without acid ion.
8. above-mentioned resin column after the rare ammonia stripping of secondary is resolved, desorbed solution adopts 5% aqueous ethanolic solution preparation, and flow is 0.4BV, and resolving to the tail sample is that 352.8ug/ml stops.Obtain desorbed solution.
9. above-mentioned desorbed solution is carried out under vacuum degree condition to thin film concentration or nanofiltration, till making concentrated unit reach 21.2 ten thousand u/ml, obtain secondary concentration liquid.
10. by above-mentioned secondary concentration liquid under agitation, slowly add 6.0mol/L analytical pure sulfuric acid liquid to be turned salt, control pH 5.8, obtain gentamicin sulfate solution; Add the medical needle shaped activated carbon that weight ratio is 1:0.1 in gentamicin sulfate solution, use the steam indirect heating, be warming up to 67 ℃ in 30min, and decoloured under the condition of insulation 45min, and, after its intracellular toxin is qualified after testing, then binder filters, obtain the de-liquid of charcoal.
The method is that gentamicin fermentation broth is through 732 resin cation (R.C.) static exchange, dilute hydrochloric acid washing, rare ammonia stripping once, dense ammonia wash-out once, once concentration, through adjusting pH, resin fixed bed dynamic adsorption, the rare ammonia stripping of secondary, parsing, secondary concentration, turning the salt decolouring, the processing steps such as spraying are produced gentamicin again.According to production method provided by the invention, not only can make gentamicin sulphate bulk drug product quality indicator reach the " requirement that Chinese pharmacopoeia is 2010 editions, and " 2010 editions gentamicin sulphate low capacity small-volume injections of Chinese pharmacopoeia are produced the specification of quality of raw materials used medicine can to make the content of each related substance meet.
The washing of 732 resin cation (R.C.) static exchange in the step of its purifying, dilute hydrochloric acid, once rare ammonia stripping, once dense ammonia wash-out, secondary concentration, turn the resins exchange that the steps such as salt decolouring, spraying drying are shown in Chinese patent the CN101648982A disclosed method of recovery gentamicin " a kind of from the waste active carbon the gentamicin sulphate decolouring ", dilute hydrochloric acid washing, rare ammonia stripping, wash-out, concentrate, turn the salt decolouring, the parameter of the steps such as spraying drying.
Adopt HPLC-light scattering detector method (HPLC-ELSD) to be measured component and the related substance of gentamicin sulphate finished product C, it is the C component concentration as a result: C
135%, C
1a28%, C
2+ C
2a37%; Its its related substances: Sissomicin 0.2%, micronomicin 1.1%, simple substance 0.5%; Always mix 1.9%, yield 71.6%.
And the once concentration liquid sample with criticizing is detected to comparison, it is the C component concentration as a result: C
132%, C
1a29%, C
2+ C
2a39%; Its its related substances: Sissomicin 1.6%, micronomicin 4.3%, single impurity 2.9%; Total assorted 8.7%.
Obviously, according to purification process provided by the invention, can make the gentamicin sulphate quality product reach the requirement of 2010 editions, and make each related substance meet the bulk drug that 2010 editions gentamicin small-volume injection qualities of production require.Its every 1,000,000,000 costs are compared with 2005 editions standards increases by 300 ~ 500 yuan, has a yield high, and quality is good, the characteristics that cost is low., and produced in conjunction with the original production equipment simultaneously, reached less investment, to realize maximum economic benefit.
Claims (4)
1. the method for a gentamicin sulphate purifying, the method is through 732 resin cation (R.C.) static exchange by the gentamicin sulphate fermented liquid, dilute hydrochloric acid, rare ammonia stripping once, dense ammonia wash-out once, once concentration, through adjusting pH, resin fixed bed dynamic adsorption, the rare ammonia stripping of secondary, wash-out, secondary concentration, turning the salt decolouring, the spray art step realizes again.
2. the method for gentamicin sulphate purifying according to claim 1 is characterized in that specifically comprising the following steps:
(1), fermented liquid is pressed into or pump in the resins exchange storage tank, according to its volume and estimation unit, saturation ratio by single 60000～90000u/ml adds 732 type resin cation (R.C.)s, in the situation that stir, exchange is more than 6 hours, then separation resin, deironing, and rinsing is clean, obtains saturated resin;
(2), by the suction of above-mentioned saturated resin sun post, pass into positive post with the washings of 0.30～0.50mol/L hydrochloric acid and washed, flow is 1.0 ~ 2.0BV, to elutant till checking without calcium, magnesium ion;
(3), by the saturated resin after above-mentioned pickling, by purified water, rinse, to go out liquid without chlorion after, the rarer ammonia that passes into 0.09 ~ 0.11mol/L carries out once rare ammonia stripping, flow is 1.0 ~ 2.0BV, till washing out and occurring after the unit of peak; And in time series connection with the purified water square impact to elutant through check the 711 type resin anion(R.A) posts without chlorion, simultaneously, the strong aqua of 3.0 ~ 3.2mol/L is passed into to positive post wash-out, cloudy post decolouring, flow is 0.4 ~ 1.0BV, and collects elutriant, the tail sample is controlled at below 1000u/ml and stops wash-out;
(4), above-mentioned elutriant is carried out under vacuum degree condition to thin film concentration or nanofiltration, till making concentrated unit reach 8 ~ 120,000 u/ml; Obtain once concentration liquid;
(5), by above-mentioned once concentration liquid under agitation, slowly add industrial sulphuric acid or hydrochloric acid to be adjusted pH7.0 ~ 7.8, obtain the gentamicin neutral solution;
(6) above-mentioned gentamicin neutral solution is pressed into or pumps into the fixed bed be equipped with through slightly acidic macroporous resin or SP series or the fine serial macroreticular resin of polypropylene and dynamically adsorbed, flow is 0.2 ~ 1.5BV, to adsorbing saturated stopping; And collect it and gone here and there post absorption containing waste liquid of effective composition.Adsorb saturated after, then replaced adsorbing saturated pillar by purified water, its waste liquid continues the absorption of string post;
(7) by above-mentioned absorption, saturated resin column carries out the rare ammonia stripping of secondary, and the rare ammonia stripping of secondary is the rare ammonia stripping of gradient; Wherein, rare ammonia concentration of gradient 1 is 0.1N, and flow is 0.5 ~ 2.0BV, till washing out waste liquid and occurring after the unit of peak; Rare ammonia concentration of gradient 2 is 0.15 ~ 0.2N, and flow is 0.5 ~ 2.0BV, till washing out waste liquid and occurring after the unit of peak; Rare ammonia concentration of gradient 3 is 0.2 ~ 0.3N, and flow is 0.5 ~ 2.0BV, till washing out waste liquid and occurring after the unit of peak; Purified water washing is near neutral or without the ammonia flavor again, and looks into the pillar outlet and stop without acid ion;
(8) above-mentioned resin column after the rare ammonia stripping of secondary is resolved, desorbed solution adopts the aqueous solution preparation of ethanol or the Virahol of 5-10%, and flow is 0.2 ~ 1.0BV, and resolving to the tail sample is that 837ug/ml stops; Obtain desorbed solution;
(9) above-mentioned desorbed solution is carried out under vacuum degree condition to thin film concentration or nanofiltration, till making concentrated unit reach 18 ~ 320,000 u/ml, obtain secondary concentration liquid;
(10) by above-mentioned secondary concentration liquid under agitation, slowly add 6.0mol/L analytical pure sulfuric acid liquid to be turned salt, control pH 5.8 ~ 6.8, obtain gentamicin sulfate solution; Add the medical needle shaped activated carbon that weight ratio is 1:0.1-0.3 in gentamicin sulfate solution, use the steam indirect heating, be warming up to 65-70 ℃ in 20-30min, and decoloured under the condition of insulation 45min, and, after its intracellular toxin is qualified after testing, then binder filters, obtain the de-liquid of charcoal;
(11), by the de-liquid of above-mentioned charcoal spraying drying after filtering, then the product of gentamicin.
3. the method for 2 gentamicin sulphate purifying according to claim 2, is characterized in that described tune pH concentrated acid used is that concentrated acid is sulfuric acid or hydrochloric acid.
4. the method for gentamicin sulphate purifying according to claim 3, is characterized in that described resin fixed bed, and its resin used is slightly acidic macroporous resin or SP series or the fine serial macroreticular resin of polypropylene.
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| CN106083952A (en) * | 2016-08-22 | 2016-11-09 | 河北圣雪大成制药有限责任公司 | A kind of method extracting gentamycin sulfate from gentamicin fermentation broth |
| CN108358984A (en) * | 2018-03-10 | 2018-08-03 | 河南省奥林特药业有限公司 | A kind of gentamicin sulphate analytic method |
| CN112409426A (en) * | 2020-11-25 | 2021-02-26 | 黑龙江格林赫思生物科技有限公司 | Preparation method of sisomicin sulfate |
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Application publication date: 20131218 |