CN106349096A - Method for preparing L-leucine from plant cells - Google Patents
Method for preparing L-leucine from plant cells Download PDFInfo
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- CN106349096A CN106349096A CN201610745955.9A CN201610745955A CN106349096A CN 106349096 A CN106349096 A CN 106349096A CN 201610745955 A CN201610745955 A CN 201610745955A CN 106349096 A CN106349096 A CN 106349096A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
- C07C227/42—Crystallisation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/07—Optical isomers
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Abstract
The invention discloses a method for preparing L-leucine from plant cells and belongs to the extraction and separation methods of intracellular products in biochemical separation engineering. The method includes: breaking plant cell walls by grinding plant tissue and using a hydrolysis method to allow the L-leucine in plant tissue cells to be dissolved in water to obtain an aqueous solution, and distilling and filtering to obtain L-leucine crystals. The method has the advantages that the method is simple and convenient to operate, the L-leucine crystals obtained by purification are high in purity and low in production cost, and the method is capable of sufficiently utilizing raw materials and saving cost.
Description
Technical field
The present invention relates in biochemical separation engineering intracellular product extraction separation method, specifically, be a kind of from plant
Cell prepares the leucic method of l.
Background technology
L- leucine, also known as leucine, chemical entitled α mono- aminoisocaproic acid, proust divides first from cheese within 1819
From obtaining.Later, braconnot obtained its crystallization from the acid hydrolysis thing of muscle and Pilus Caprae seu Oviss, and named as leucine.L mono- is bright
Propylhomoserin is one of common 18 kinds of aminoacid, is also one of eight kinds of essential amino acids of human body；L- leucine belongs to required amino
Acid, man's requirement 2.2g/d, is baby's normal growth and adult's normal nitrogen balance of maintenance is required, and this product is as nutrition
Enriching substance, prepares amino acid transfusion and comprehensive amino acid preparation, Hypoylycemic agents, plant growth promoter, by China gb 2760-
86 regulations, this product can be used as spice, as amino acid transfusion and comprehensive amino acid preparation, for examining of child's idiopathic hyperglycemia
Break and treat, and be applied to carbohydrate metabolism imbalance, the hepatopathy with bile secretion minimizing, anemia, poisoning, muscular atrophy, spinal cord ash
Matter inflammation sequela, neuritiss and psychosiss.
L leucine of the prior art has obtained industrialized production, mainly include chemical synthesiss, enzyme catalysis method,
Proteolysis method, microbe fermentation method, during acquisition l is leucic, the step that it produces is various, mistake for said method
Journey is complicated.
Content of the invention
It is an object of the invention to provide one kind prepares the leucic method of l from plant cell, its operating procedure is simple,
Convenient to carry out, save the cost input in manufacture process, practicality is higher.
The present invention is achieved through the following technical solutions: one kind prepares the leucic method of l from plant cell, including following
Step:
Step 1: take plant tissue to be positioned on the hard platform of a flat smooth, plant tissue takes the mode uniformly keeping flat
It is placed on above-mentioned platform, may move hard pressing plate using an another one corresponding with above-mentioned hard platform and grind plant group
Knit, add water during grinding, obtain plant cell lapping liquid and grind residue.
Step 2: take the lapping liquid obtaining from step 1 to be placed in boiler, heating distillation, obtain plant cytoplasm crystallization
Body, and collected and be placed in container.
Step 3: the plant cell obtaining from step 1 grinding residue is placed in saline, stands 5 10 minutes, after taking-up
It is placed in clear water, stand 5 10 minutes, obtain mixed solution again.
Step 4: the mixed solution obtaining from step 3 is placed in distillator, heating distillation, obtains mixed crystallization body, and
Collected and be placed in container.
Step 5: take from the plant cytoplasm crystalline solid that step 2 obtains and from step 4 the mixed crystallization body that obtains in the lump
Insert in concentration tank, be passed through ammonia, and heat distillation, heating-up temperature is 80 degrees Celsius, distillation time is 4h, obtains distillate.
Step 6: the distillate obtaining from step 5 is filtrated to get filtrate and residue.
Step 7: the residue obtaining from step 6 clear water is dissolved, then by solution through row second distillation, heating-up temperature is 80
Degree Celsius, distillation time is 1h, obtains second distillation liquid, is filtered, obtains l leucine crystal.
Step 8: above-mentioned l leucine crystal is utilized distilled water flushing, and centrifuge dripping, send into dehydrator, be passed through steaming
Vapour is dried, packaging, warehouse-in, drying temperature 100, drying time 3h.
Further, in order to preferably realize the present invention, process of lapping described in step 1, it is applied to removable hard pressure
Power on plate is 5 10n, and milling time is 10 20min；Described add water during, the flow-control of water is in 100ml/min.
Further, in order to preferably realize the present invention, still-process described in step 2, to reach aneroid in boiler
Till body, vapo(u)rizing temperature is 80 degrees Celsius.
Further, include fine mesh gauze to preferably realize defecator used in the present invention, step 6, it is thin
Bore dia is 0.1mm, during filtering, constantly rocks defecator, till liquid and crystal are separated.
Further, in order to preferably realize the present invention, the concentration of described ammonia is mass fraction 50%.
The present invention compared with prior art, has advantages below and a beneficial effect:
(1) operating procedure of the present invention is simple, convenient to carry out, and the cost that its manufacture process puts into is few, thus cost-effective
Put into, strengthen the practicality of the present invention；
(2) the l leucine purity that the present invention is obtained by way of multiple distillation is high, and using rocking in filter process
Mode, so that l leucine crystal is higher with moisture separation efficiency, and then saves the production time.
(3) present invention will grind the liquid obtaining and plant residue all using preparing l leucine, thus at utmost
Utilization raw material, thus economizing on resources.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this.
Embodiment 1:
In the present embodiment, one kind prepares the leucic method of l from plant cell, comprises the following steps:
Step 1: take plant tissue to be positioned on the hard platform of a flat smooth, plant tissue takes the mode uniformly keeping flat
It is placed on above-mentioned platform, may move hard pressing plate using an another one corresponding with above-mentioned hard platform and grind plant group
Knit, add water during grinding, obtain plant cell lapping liquid and grind residue.
Step 2: take the lapping liquid obtaining from step 1 to be placed in boiler, heating distillation, obtain plant cytoplasm crystallization
Body, and collected and be placed in container.
Step 3: the plant cell obtaining from step 1 grinding residue is placed in saline, stands 5 10 minutes, after taking-up
It is placed in clear water, stand 5 10 minutes, obtain mixed solution again.
Step 4: the mixed solution obtaining from step 3 is placed in distillator, heating distillation, obtains mixed crystallization body, and
Collected and be placed in container.
Step 5: take from the plant cytoplasm crystalline solid that step 2 obtains and from step 4 the mixed crystallization body that obtains in the lump
Insert in concentration tank, be passed through ammonia, and heat distillation, heating-up temperature is 80 degrees Celsius, distillation time is 4h, obtains distillate.
Step 6: the distillate obtaining from step 5 is filtrated to get filtrate and residue.
Step 7: the residue obtaining from step 6 clear water is dissolved, then by solution through row second distillation, heating-up temperature is 80
Degree Celsius, distillation time is 1h, obtains second distillation liquid, is filtered, obtains l leucine crystal.
Step 8: above-mentioned l leucine crystal is utilized distilled water flushing, and centrifuge dripping, send into dehydrator, be passed through steaming
Vapour is dried, packaging, warehouse-in, drying temperature 100, drying time 3h.
The present embodiment provided a kind of from plant cell by grind by way of prepare the leucic method of l, its tool
Have simple to operate, cost input is low, the l leucine purity of preparation is high, meets national standard；Equipment needed thereby is few.
In the present embodiment, the principle of preparation is: takes the method grinding plant tissue first, by Activities of Some Plants cytoclasises
Fall, and be dissolved in water so that the endolysis in plant cell, in water, obtains mixed solution, because in plant cytoplasm
Containing l leucine, therefore adopt said method, l leucine solution can be obtained；In addition, the residue of gained will be ground, lead to
Cross Hydrolyze method, destroy wherein complete cell wall so as in l leucine overflow from plant cell wall；Complete above-mentioned side
After method, then l leucine crystal is obtained by the method for distillation, filtration.
Embodiment 2:
The present embodiment as the specific embodiment of embodiment 1, process of lapping described in step 1, be applied to removable hard pressure
Power on plate is 5 10n, and milling time is 10 20min；Described add water during, the flow-control of water is in 100ml/min.
Using above-mentioned design, apply the power of 5 10n, and grind 10 20min it is ensured that in process of lapping, plant is thin
While cell wall is destroyed, the composition in Cytoplasm will not be destroyed；The process being dissolved in water, can make the one-tenth in plant cytoplasm
Divide and be dissolved in water, be easy to subsequent step and therefrom isolate l leucine, it is simple to operate, convenient to carry out.
Embodiment 3:
, as the specific embodiment of embodiment 1, still-process described in step 2, to reach aneroid in boiler for the present embodiment
Till body, vapo(u)rizing temperature is 80 degrees Celsius.
Using above-mentioned design, when predistillation being made, the step of saving filtration, due in cytoplasmic components, respectively
The boiling point portion of component is the same, therefore adopts said method, can preliminary purification Cytoplasm solution, and by it with remaining composition of plant cell
Separate with Cytoplasm.
Embodiment 4:
As the specific embodiment of embodiment 1, used in step 6, defecator includes fine mesh gauze to the present embodiment, and it is thin
Bore dia is 0.1mm, during filtering, constantly rocks defecator, till liquid and crystal are separated.
Using said method, l leucine crystal can be made higher with moisture separation efficiency, and then save the production time,
And fine mesh gauze can stop that the l leucine having formed crystal exposes from gauze, while realizing filtering purpose, protect
Demonstrate,prove the leucic yield of its l.
Embodiment 5:
As the specific embodiment of above-described embodiment, the concentration of above-mentioned ammonia is 50%(mass to the present embodiment).Using above-mentioned side
Method, can make the leucic purification process of l more quick, improve its production efficiency, and then strengthen the practicality of the present invention.
The above, be only presently preferred embodiments of the present invention, and not the present invention is done with any pro forma restriction, every according to
Any simple modification above example made according to the technical spirit of the present invention, equivalent variations, each fall within the protection of the present invention
Within the scope of.
Claims (5)
1. one kind from plant cell prepare the leucic method of l it is characterised in that: comprise the following steps:
Step 1: take plant tissue to be positioned on the hard platform of a flat smooth, plant tissue takes the mode uniformly keeping flat
It is placed on above-mentioned platform, may move hard pressing plate using an another one corresponding with above-mentioned hard platform and grind plant group
Knit, add water during grinding, obtain plant cell lapping liquid and grind residue；
Step 2: take the lapping liquid obtaining from step 1 to be placed in boiler, heating distillation, obtain plant cytoplasm crystalline solid, and
Collected and be placed in container；
Step 3: the plant cell obtaining from step 1 grinding residue is placed in saline, stands 5 10 minutes, again will after taking-up
It is placed in clear water, stands 5 10 minutes, obtains mixed solution；
Step 4: the mixed solution obtaining from step 3 is placed in distillator, heating distillation, obtain mixed crystallization body, and by its
Collection is placed in container；
Step 5: take from the plant cytoplasm crystalline solid that step 2 obtains and from step 4 the mixed crystallization body one that obtains be placed into
In concentration tank, it is passed through ammonia, and heats distillation, heating-up temperature is 80 degrees Celsius, distillation time is 4h, obtains distillate；
Step 6: the distillate obtaining from step 5 is filtrated to get filtrate and residue；
Step 7: the residue obtaining from step 6 clear water is dissolved, then by solution through row second distillation, heating-up temperature is Celsius for 80
Degree, distillation time is 1h, obtains second distillation liquid, is filtered, obtains l leucine crystal；
Step 8: above-mentioned l leucine crystal is utilized distilled water flushing, and centrifuge dripping, send into dehydrator, be passed through steam and dry
Dry, packaging, warehouse-in, drying temperature 100, drying time 3h.
2. according to claim 1 a kind of prepare the leucic method of l from plant cell it is characterised in that: step 1
Described process of lapping, the power being applied on removable hard pressing plate is 5 10n, and milling time is 10 20min；Described add water
During, the flow-control of water is in 100ml/min.
3. according to claim 1 a kind of prepare the leucic method of l from plant cell it is characterised in that: step 2
Described still-process, reaching no liquid in boiler, vapo(u)rizing temperature is 80 degrees Celsius.
4. according to claim 1 a kind of prepare the leucic method of l from plant cell it is characterised in that: step 6
The defecator using includes fine mesh gauze, and its pore diameter is 0.1mm, during filtering, constantly rocks defecator,
So that liquid and crystal to be separated.
5. a kind of method preparing the bright ammonia of l from plant cell according to any one of claim 14, its feature exists
In: the concentration of described ammonia is mass fraction 50%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610745955.9A CN106349096A (en) | 2016-08-29 | 2016-08-29 | Method for preparing L-leucine from plant cells |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610745955.9A CN106349096A (en) | 2016-08-29 | 2016-08-29 | Method for preparing L-leucine from plant cells |
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| CN106349096A true CN106349096A (en) | 2017-01-25 |
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| CN201610745955.9A Pending CN106349096A (en) | 2016-08-29 | 2016-08-29 | Method for preparing L-leucine from plant cells |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114773216A (en) * | 2022-03-24 | 2022-07-22 | 天津大学 | L-leucine spherical crystal and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922951B (en) * | 2014-05-06 | 2015-06-17 | 鲁成宪 | Method for extracting leucine by utilizing corn protein powder |
-
2016
- 2016-08-29 CN CN201610745955.9A patent/CN106349096A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922951B (en) * | 2014-05-06 | 2015-06-17 | 鲁成宪 | Method for extracting leucine by utilizing corn protein powder |
Non-Patent Citations (1)
| Title |
|---|
| 孙中武 编著: "《植物化学》", 30 April 2001, 东北林业大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114773216A (en) * | 2022-03-24 | 2022-07-22 | 天津大学 | L-leucine spherical crystal and preparation method and application thereof |
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