CN106342808A - Application of ascaroside C9 in prevention and control of monochamus alternates and intercepting of spreading of pine wood nematode - Google Patents
Application of ascaroside C9 in prevention and control of monochamus alternates and intercepting of spreading of pine wood nematode Download PDFInfo
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- CN106342808A CN106342808A CN201610620708.6A CN201610620708A CN106342808A CN 106342808 A CN106342808 A CN 106342808A CN 201610620708 A CN201610620708 A CN 201610620708A CN 106342808 A CN106342808 A CN 106342808A
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- alternatus
- ascarid
- glycoside
- bursaphelenchus xylophilus
- ascarid glycoside
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Abstract
The invention relates to application of ascaroside C9 in prevention and control of monochamus alternates and intercepting of spreading of pine wood nematode. The method for preventing and controlling the monochamus alternates by using the ascaroside C9 comprises the following step: placing the ascaroside C9 in a pine tree containing monochamus alternates larvae. The method provided by the invention can be used for treating forest monochamus alternates and pine trees infected with the pine wood nematode. The problems that the population quantity of the monochamus alternates in a pine wood nematode epidemic place is large, hazards are severe, and the pine wood nematode is diffused rapidly by the monochamus alternates can be solved.
Description
Technical field
The present invention relates to ascarid glycoside c9 in Monochamus alternatus preventing and treating and stops the application during Bursaphelenchus xylophilus are propagated.
Background technology
Monochamus alternatus occur and few in the non-generating region of Bursaphelenchus xylophilus, and harm is not enough to attract people's attention.Bursaphelenchus xylophilus
After intruding into China, work in coordination with interaction with medium Monochamus alternatus, both population densities are all greatly improved, form expansion and spread rapidly
Trend.
Pine nematode, is referred to as " cancer " of pinaster, is forest disease most dangerous in the world, is pinaceae plant
Crushing epidemic disease, host plant infect this after being ill 2 March can death, full wafer pine forest, hazardness pole can be destroyed within 35 years
By force.From nineteen eighty-two since Nanjing finds pine nematode first, epidemic situation diffusion is rapid for China, spread to including Jiangsu, Anhui,
14 provinces and cities such as Guangdong, Guangxi, Zhejiang, Fujian, Shanghai, Chongqing, Hubei, Shandong, 86 counties and cities.Over 28 years, add up lethal pine
Set more than 500,000,000 plant, destroy more than 500 ten thousand mu of pine forest, cause the economic loss of hundreds billion of units, and occurrence scope is also expanding year by year.
It not only brings about great losses to national economy, also destroys natural landscape and ecological environment, the pine forest resource to China's abundant
Constitute serious threat.This harmful destructiveness of being critically ill is: pathogenicity is strong, and host's death rate is fast；Propagate fast, and usually sudden not
And it is anti-；Once occurring, difficulty of governance is big.At present, to pine nematode, rely primarily on the ill tree of removing institute and administered, but
Because the particularity of its cause of disease biocycle, DeGrain.Therefore, suitable from Infection cycie and diffusion mechanism set off in search
Prevention and controls become the task of top priority.
The cause of disease of pine nematode is Bursaphelenchus xylophilus, is one of most important Alien invasive species of China.It cannot itself be complete
Become Spreading and diffusion task, the Spreading and diffusion between pinaster must rely on insect vector Monochamus alternatus carry to complete.Woodland
The first disease time of pine nematode typically at the beginning of 6 months, 7, August part be pine nematode onset peak.Invaded by Bursaphelenchus xylophilus
The pinaster having contaminated is the place that Monochamus alternatus are liked laying eggs.The month in coming year 1-5, Bursaphelenchus xylophilus enter diapause, form diffused pine
Nematicide.Cast off a skin first and form diffused third-instar larvae (lⅲ), and be gathered in around Monochamus alternatus pupa room, June is with Monochamus alternatus
Emergence and form diffused four-age larva (lⅳ) and enter Monochamus alternatus pore and be carried and travel to new healthy pinaster, start
The Infection cycie of a new round.lⅳResin canal is entered by caused on bark wound when Monochamus alternatus Hope feeding or spawning, so
After cast off a skin formation adult, these are female, lay eggs after male imago mating, be changed into reproduction type pine wood nematode after egg hatching and amount reproduction and
Withered flavescence becomes the object that Monochamus alternatus are laid eggs again quickly to make pinaster, and the alternatus Larva of spawning and hatching enters pine again
Take food burrow in nematicide tree, form pupa room.Both cooperation, complete condition needed for life cycle provides for other side, and utilize
The condition that other side creates completes itself and develops.In a word, diffused four-age larva (lⅳ) time of being formed is when sprouting wings with Monochamus alternatus
Between to agree be the key that Bursaphelenchus xylophilus are carried propagation.Carrying out obstruction to the diffusive transport of Bursaphelenchus xylophilus is prevention and control Bursaphelenchus xylophilus
Key.Therefore, the growth to alternatus Larva is disturbed and is regulated and controled, can by Monochamus alternatus emergence time postpone or
Cannot sprout wings, so that lⅳCannot form and cut off the approach of its Spreading and diffusion.
Content of the invention
In order to realize the obstruction to Monochamus alternatus preventing and treating and pine nematode diffusive transport, the present invention provides a kind of new prevention and control
Method.
Technical scheme is as follows:
Ascarid glycoside c9 is used for Monochamus alternatus preventing and treating and stops Bursaphelenchus xylophilus propagation by the present invention.
Provided by the present invention ascarid glycoside c9 is used for preventing and controlling pine wilt disease method be: ascarid glycoside c9 is placed in containing Song Motian
In the pinaster of cattle larva.
As optimization, the method that ascarid glycoside c9 is used for Monochamus alternatus preventing and treating and stops Bursaphelenchus xylophilus propagation provided by the present invention
For: ascarid glycoside c9 is adsorbed in the pinaster in the carrier possessing adsorptivity and slow release effect, this carrier being placed in alternatus Larva
In.The described carrier possessing adsorptivity and slow release effect is silicone rubber chock plug.
As optimization, the method that ascarid glycoside c9 is used for Monochamus alternatus preventing and treating and stops Bursaphelenchus xylophilus propagation provided by the present invention
For:
1), ascarid glycoside c9 and organic solvent are mixed, obtain mixed liquor；
2), by step 1) gained mixed liquor 20 μ l contacted with the carrier possessing adsorptivity and slow release effect, treats organic solvent
After volatilization, this carrier is placed in the pinaster containing alternatus Larva.
Described organic solvent is selected from methanol, ethanol or ether.
As optimization, provided by the present invention ascarid glycoside c9 is used in the method for preventing and controlling pine wilt disease, the consumption of ascarid glycoside c9
For: 150nm-150 μm/m3Pinaster body.
What the present invention was realized has the beneficial effect that:
Ascarid glycoside c9 of the present invention is artificial sinteticses, its Stability Analysis of Structures, stable chemical nature under normal temperature and pressure,
Nontoxic, free from environmental pollution.
The method operation letter that ascarid glycoside c9 is used for Monochamus alternatus preventing and treating and stops Bursaphelenchus xylophilus propagation provided by the present invention
Single it is easy to popularization and application.
Provided by the present invention by ascarid glycoside c9 be used for Monochamus alternatus preventing and treating and stop Bursaphelenchus xylophilus propagate method to pine ink
The prevention effect of Anoplophorae seu Aprionae preventing and treating and obstruction Bursaphelenchus xylophilus propagation is obvious.
Specific embodiment
Ascarid glycoside c9 of the present invention chemically synthesizes (as Tianjin Yaoming Kangde new drugs development Co., Ltd).English name
ascarosides(asc-c9).Molecular formula: c15h28o6.Its Stability Analysis of Structures, stable chemical nature under normal temperature and pressure, nontoxic, not dirty
Dye environment.Structural formula is:Synthetic route is shown in document: ascaroside activity
in caenorhabditis elegans is highly dependent on chemical
Structure.bioorganic＆medicinal chemistry (2013) 21:5754 5769.
Silicone rubber chock plug, is purchased from Pherobio Technology Co., Ltd., the limited public affairs of Beijing Ge Ruibi source science and technology
Department etc..
Embodiment 1:
In Bursaphelenchus xylophilus generating region, Monochamus alternatus at the beginning of 5 months by the end of April are pupated before beginning, select 40 plants to contain multiple pine ink skies
The Pinus massoniana Lamb of cattle larva.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have Monochamus alternatus oviposition cut mark
Vestige, part Folium Pini chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, tree body sunny slope xylem pine ink sky
Cattle eats into bores the deep hole of 1cm diameter 2cm into hole electric drill, communicates with alternatus Larva pupa room.It is directly added into in experimental group hole
Ascarid glycoside c9 dosage 6nm/ hole, ascarid glycoside c9 consumption is 30nm/m3Pinaster body.Matched group, without ascarid glycoside c9, after 10 days, cuts Song Mo
Roundheaded borer is observed.Result shows, the Monochamus alternatus of matched group 80% are pupa, and experimental group 50% is pupa, and 50% is children
Worm.
Embodiment 2:
Ascarid glycoside c9 is dissolved in methanol, is adsorbed in silicone rubber chock plug (rubber septa) (0.5cm diameter, 1cm length).In pine
Material nematicide generating region, selects 40 plants of Pinus massoniana Lamb containing multiple alternatus Larvas.Recognition methodss are: have Bursaphelenchus xylophilus harm
Not resin flux after pinaster boring, has Monochamus alternatus oviposition cut mark vestige, part Folium Pini chlorosis yellow.20 plants as experimental group, 20 plants
As a control group, eat in tree body sunny slope xylem Monochamus alternatus and bore the deep hole of 1cm diameter 2cm into hole electric drill.Experimental group will
The some dispersions of silicone rubber chock plug of the c91 μ l of glycoside containing ascarid insert in the hole and (after 5 μ l ascarid glycoside c9 and 100 μ l methanol mixed, take 20 μ l to drip
Enter silicone rubber chock plug, can use after solvent volatilization), ascarid glycoside c9 consumption is 5 μ l/m3Pinaster body.Matched group insertion is containing methanol 1 μ l's
Silicone rubber chock plug, after 10 days, cuts alternatus Larva and is observed.Result shows, the Monochamus alternatus of matched group 82% are pupa,
And experimental group 35% is pupa, 65% is larva.
Embodiment 3:
Ascarid glycoside c9 is dissolved in ethanol, is adsorbed in silicone rubber chock plug (0.5cm diameter, 1cm length).In Bursaphelenchus xylophilus generating region,
Select 40 plants of Pinus massoniana Lamb containing multiple alternatus Larvas.Recognition methodss are: after having the pinaster that Bursaphelenchus xylophilus endanger to hole not
Resin flux, has Monochamus alternatus oviposition cut mark vestige, part Folium Pini chlorosis yellow.20 plants as experimental group, 20 plants are as a control group,
Tree body sunny slope xylem Monochamus alternatus are eaten into and are bored the deep hole of 1cm diameter 2cm into hole electric drill.Experimental group is by the silicon rubber of the c9 of glycoside containing ascarid
The some dispersions of plug head insert in the hole and (take 20 μ l to instill silicone rubber chock plug 3 μm of ascarid glycoside c9 ethanols, can make after solvent volatilization
With), ascarid glycoside c9 consumption is 15 μm/m3Pinaster body.The silicone rubber chock plug containing ethanol 20 μ l for the matched group insertion, after 10 days, cuts pine
Black roundheaded borer is observed.Result shows, the Monochamus alternatus of matched group 83% are pupa, and experimental group 20% develops pupa,
80% is larva.
Embodiment 4:
Ascarid glycoside c9 is dissolved in ether, is adsorbed in silicone rubber chock plug (0.5cm diameter, 1cm length).In Bursaphelenchus xylophilus generating region,
Select 40 plants of Pinus massoniana Lamb containing multiple alternatus Larvas.Recognition methodss are: after having the pinaster that Bursaphelenchus xylophilus endanger to hole not
Resin flux, has Monochamus alternatus oviposition cut mark vestige, part Folium Pini chlorosis yellow.20 plants as experimental group, 20 plants are as a control group,
Tree body sunny slope xylem Monochamus alternatus are eaten into and are bored the deep hole of 1cm diameter 2cm into hole electric drill.Experimental group is by the silicon rubber of the c9 of glycoside containing ascarid
The some dispersions of plug head insert in the hole and (take 20 μ l to instill silicone rubber chock plug 3 μm of ascarid glycoside c9 ether solutions, take 20 μ l to instill silicone rubber
Chock plug, can use after solvent volatilization), ascarid glycoside c9 consumption is 15 μm/m3Pinaster body.The silicone rubber containing ether 20 μ l for the matched group insertion
Chock plug, after 10 days, cuts alternatus Larva and is observed.Result shows, the Monochamus alternatus of matched group 81% are pupa, and test
Group 35% growth pupa, 65% is larva.
Embodiment 5:
Ascarid glycoside c9 is dissolved in ethanol, is adsorbed in Cotton Gossypii (0.5cm diameter, 1cm length).In Bursaphelenchus xylophilus generating region, select 40
The Pinus massoniana Lamb containing multiple alternatus Larvas for the strain.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have
Monochamus alternatus oviposition cut mark vestige, part Folium Pini chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, tree body to
Sunny side xylem Monochamus alternatus are eaten into and are bored the deep hole of 1cm diameter 2cm into hole electric drill.Experimental group is by some points of the Cotton Gossypii of the c9 of glycoside containing ascarid
Dissipate insert in the hole (taking 20 μ l to instill Cotton Gossypii 3 μm of ascarid glycoside c9 ethanols, can use after solvent volatilization), ascarid glycoside c9 consumption is 15 μ
m/m3Pinaster body.The Cotton Gossypii containing methanol 20 μ l for the matched group insertion, after 10 days, cuts alternatus Larva and is observed.Result shows
Show, the Monochamus alternatus of experimental group 48% are pupa, and matched group 86% develops pupa, and 14% is larva.
Embodiment 6:
Ascarid glycoside c9 is adsorbed in Linesless charcoal (0.5cm diameter, 1cm length).In Bursaphelenchus xylophilus generating region, select 40 plants containing multiple
The Pinus massoniana Lamb of alternatus Larva.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have Monochamus alternatus to produce
Ovum cutting vestige, part Folium Pini chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, in tree body sunny slope xylem
Monochamus alternatus are eaten into and are bored the deep hole of 1cm diameter 2cm into hole electric drill.The some dispersions of Linesless charcoal of the c9 of glycoside containing ascarid are inserted in the hole by experimental group
(taking 20 μ l to instill Linesless charcoal 3 μm of ascarid glycoside c9 ethanols, can use after solvent volatilization), ascarid glycoside c9 consumption is 15 μm/m3Pinaster body.
The Cotton Gossypii containing methanol 20 μ l for the matched group insertion, after 10 days, cuts alternatus Larva and is observed.Result shows, experimental group
44% Monochamus alternatus are pupa, and matched group 85% develops pupa, and 15% is larva.
Embodiment 7:
By ascarid glycoside c9 DM.In Bursaphelenchus xylophilus generating region, select 40 plants of horse hairs containing multiple alternatus Larvas
Pine.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have Monochamus alternatus oviposition cut mark vestige, partly pine
Pin chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, eat into into hole electricity consumption in tree body sunny slope xylem Monochamus alternatus
Bore the deep hole of 1cm diameter 2cm.Experimental group inserts in the hole some dispersions of silicone rubber chock plug of the c9 of glycoside containing ascarid 20 μ l (by 3 μm
Ascarid glycoside c9 ethanol, takes 20 μ l to instill silicone rubber chock plug, can use after solvent volatilization), ascarid glycoside c9 consumption is 15 μm/m3Pinaster
Body.The silicone rubber chock plug containing methanol 10 μ l for the matched group insertion, after 10 days, cuts alternatus Larva and is observed.Result shows,
The Monochamus alternatus of experimental group 46% are pupa, and matched group 83% develops pupa, and 17% is larva.
Embodiment 8:
By ascarid glycoside c9 DM.In Bursaphelenchus xylophilus generating region, select 40 plants of horse hairs containing multiple alternatus Larvas
Pine.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have Monochamus alternatus oviposition cut mark vestige, partly pine
Pin chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, eat into into hole electricity consumption in tree body sunny slope xylem Monochamus alternatus
Bore the deep hole of 1cm diameter 2cm.Experimental group inserts in the hole some dispersions of silicone rubber chock plug of the c9 of glycoside containing ascarid 20 μ l (by 3 μm
Ascarid glycoside c9 ethanol, takes 20 μ l to instill silicone rubber chock plug, can use after solvent volatilization), ascarid glycoside c9 consumption is 150 μm/m3Pinaster
Body.The silicone rubber chock plug containing ethanol 20 μ l for the matched group insertion, after 10 days, cuts alternatus Larva and is observed.Result shows,
The Monochamus alternatus of experimental group 30% are pupa, and matched group 83% develops pupa, and 17% is larva.
Embodiment 9:
By ascarid glycoside c9 DM.In Bursaphelenchus xylophilus generating region, select 40 plants of horse hairs containing multiple alternatus Larvas
Pine.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have Monochamus alternatus oviposition cut mark vestige, partly pine
Pin chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, eat into into hole electricity consumption in tree body sunny slope xylem Monochamus alternatus
Bore the deep hole of 1cm diameter 2cm.The some dispersions of silicone rubber chock plug of the c9 of glycoside containing ascarid 20 μ l are inserted in the hole by experimental group (will
300nm ascarid glycoside c9 ethanol, takes 20 μ l to instill silicone rubber chock plug, can use after solvent volatilization), ascarid glycoside c9 consumption is 1.5 μm/m3
Pinaster body.The silicone rubber chock plug containing ethanol 20 μ l for the matched group insertion, after 10 days, cuts alternatus Larva and is observed.Result
Display, the Monochamus alternatus of experimental group 26% are pupa, and matched group 83% develops pupa, and 17% is larva.
Embodiment 10:
By ascarid glycoside c9 DM.In Bursaphelenchus xylophilus generating region, select 40 plants of horse hairs containing multiple alternatus Larvas
Pine.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have Monochamus alternatus oviposition cut mark vestige, partly pine
Pin chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, eat into into hole electricity consumption in tree body sunny slope xylem Monochamus alternatus
Bore the deep hole of 1cm diameter 2cm.Experimental group inserts in the hole some dispersions of silicone rubber chock plug of the c9 of glycoside containing ascarid 20 μ l (by 30nm
Ascarid glycoside c9 ethanol, take 20 μ l to instill silicone rubber chock plug, can use after solvent volatilization), ascarid glycoside c9 consumption is 150nm/m3Pinaster
Body.The silicone rubber chock plug containing ethanol 20 μ l for the matched group insertion, after 10 days, cuts alternatus Larva and is observed.Result shows,
The Monochamus alternatus of experimental group 30% are larva, and matched group 83% develops pupa, and 17% is larva.
Embodiment 11:
By ascarid glycoside c9 DM.In Bursaphelenchus xylophilus generating region, select 40 plants of horse hairs containing multiple alternatus Larvas
Pine.Recognition methodss are: have not resin flux after the pinaster boring of Bursaphelenchus xylophilus harm, have Monochamus alternatus oviposition cut mark vestige, partly pine
Pin chlorosis yellow.20 plants as experimental group, 20 plants are as a control group, eat into into hole electricity consumption in tree body sunny slope xylem Monochamus alternatus
Bore the deep hole of 1cm diameter 2cm.Experimental group inserts in the hole some dispersions of silicone rubber chock plug of the c9 of glycoside containing ascarid 20 μ l (by 3nm
Ascarid glycoside c9 ethanol, takes 20 μ l to instill silicone rubber chock plug, can use after solvent volatilization), ascarid glycoside c9 consumption is 15nm/m3Pinaster
Body.The silicone rubber chock plug containing ethanol 20 μ l for the matched group insertion, after 10 days, cuts alternatus Larva and is observed.Result shows,
The Monochamus alternatus of experimental group 45% are pupa, and matched group 83% develops pupa, and 17% is larva.
Under field conditions (factors), diffused Bursaphelenchus xylophilus are to be formed in a large number in the 1-5 month in the coming year, and loose with insect vector
Black Anoplophorae seu Aprionae emergence time is consistent, can be carried by Monochamus alternatus and travel to new healthy pinaster.Carry by using the present invention
For method, during annual May to July, at the beginning of 6 months of especially just morbidity, alternatus Larva can be made to stop sending out
Educate, reduce Bursaphelenchus xylophilus pupa and adult population density, reduce pine death rate, meanwhile, make the Bursaphelenchus xylophilus cannot be with Song Motian
The emergence of cattle and form diffused four ages nematicide, and block its diffusive transport, and then it be propagated further diffusion to reach interference
Target, thus realizing reducing Bursaphelenchus xylophilus generating region pine death rate and diffusion rate of propagation, and prevents climing further in forest zone
Prolong the prevention and control target of extension.
The method that the present invention provides can be utilized administers in the pinaster of the infected Bursaphelenchus xylophilus of woodland.Can solve in pine line
The Bursaphelenchus xylophilus amount reproduction of worm epidemic situation spot occurs and the rapid problem spreading.
Claims (7)
1. ascarid glycoside c9 in preventing and treating Monochamus alternatus and stops the application during Bursaphelenchus xylophilus are propagated.
2. the method being used for preventing and treating Monochamus alternatus and stopping Bursaphelenchus xylophilus propagation by ascarid glycoside c9: ascarid glycoside c9 is placed in containing Song Motian
In the pinaster of cattle larva.
3. described in claim 2, ascarid glycoside c9 is used for preventing and treating Monochamus alternatus and stopping the method that Bursaphelenchus xylophilus are propagated, its feature exists
In ascarid glycoside c9 being adsorbed in the carrier possessing adsorptivity and slow release effect, this carrier is placed in containing alternatus Larva
In pinaster.
4. described in claim 3, ascarid glycoside c9 is used for preventing and treating Monochamus alternatus and stopping the method that Bursaphelenchus xylophilus are propagated, its feature exists
In the described carrier possessing adsorptivity and slow release effect is silicone rubber chock plug.
5. described in claim 3, ascarid glycoside c9 is used for preventing and treating Monochamus alternatus and stopping the method that Bursaphelenchus xylophilus are propagated, its feature exists
In step is as follows:
1), ascarid glycoside c9 is mixed with organic solvent, obtain mixed liquor；
2), by step 1) gained mixed liquor contacted with the carrier possessing adsorptivity and slow release effect, after organic solvent volatilization, will
This carrier is placed in the pinaster containing alternatus Larva.
6. described in claim 5, ascarid glycoside c9 is used for the method for alternatus Larva preventing and treating it is characterised in that described organic solvent
Selected from methanol, ethanol or ether.
7. described in claim 2-6 any one, ascarid glycoside c9 is used for preventing and treating Monochamus alternatus and stopping the side that Bursaphelenchus xylophilus are propagated
Method is it is characterised in that the consumption of ascarid glycoside c9 is: 150nm-150 μm/m3Pinaster body.
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| CN201610620708.6A CN106342808A (en) | 2016-07-29 | 2016-07-29 | Application of ascaroside C9 in prevention and control of monochamus alternates and intercepting of spreading of pine wood nematode |
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