CN106234362B - Ascarid glucoside △ C9 prevents and treats and stops the application in Bursaphelenchus xylophilus propagation in Monochamus alternatus - Google Patents
Ascarid glucoside △ C9 prevents and treats and stops the application in Bursaphelenchus xylophilus propagation in Monochamus alternatus Download PDFInfo
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- CN106234362B CN106234362B CN201610619652.2A CN201610619652A CN106234362B CN 106234362 B CN106234362 B CN 106234362B CN 201610619652 A CN201610619652 A CN 201610619652A CN 106234362 B CN106234362 B CN 106234362B
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- monochamus alternatus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Abstract
The present invention relates to methods ascarid glucoside △ C9 prevention and treatment Monochamus alternatus and stop Bursaphelenchus xylophilus propagation.The method provided by the present invention that ascarid glucoside △ C9 is used for Monochamus alternatus prevention and treatment are as follows: ascarid glucoside △ C9 is placed in the pine tree containing alternatus Larva.Method provided by the invention is administered using in the pine tree of woodland Monochamus alternatus and infection Bursaphelenchus xylophilus.It can solve the problems, such as the Monochamus alternatus population quantity of pine wilt disease spot is big, harm weight and Bursaphelenchus xylophilus are spread rapidly by Monochamus alternatus.
Description
Technical field
The present invention relates to ascarid glucoside △ C9 to prevent and treat and stop the application in Bursaphelenchus xylophilus propagation in Monochamus alternatus.
Background technique
Monochamus alternatus occurs and few in the non-generating region of Bursaphelenchus xylophilus, and harm is not enough to attract people's attention.Bursaphelenchus xylophilus
After intruding into China, interaction is cooperateed with medium Monochamus alternatus, the population density of the two all greatly improves, and it is rapid to form expansion sprawling
Trend.
Pine nematode, referred to as " cancer " of pine tree are the most forest diseases of risk in the world, are pinaceae plants
Crushing epidemic disease, host plant infects this, and 2-March can be dead after being ill, can destroy within 3-5 years full wafer pine forest, harmfulness pole
By force.China from nineteen eighty-two since Nanjing finds pine nematode for the first time, epidemic situation diffusion rapidly, spread to including Jiangsu, Anhui,
14 provinces and cities such as Guangdong, Guangxi, Zhejiang, Fujian, Shanghai, Chongqing, Hubei, Shandong, 86 counties and cities.Over 28 years, add up lethal pine
More than 500,000,000 plants are set, more than 500 ten thousand mu of pine forest is destroyed, causes the economic loss of hundreds billion of members, and occurrence scope is also expanding year by year.
It not only brings about great losses to national economy, also destroys natural landscape and ecological environment, to China's pine forest resource abundant
It constitutes and seriously threatens.The harmful destructiveness of being critically ill is: pathogenicity is strong, and host's death rate is fast；Propagate it is fast, and usually it is sudden not
And it is anti-；Once occurring, difficulty of governance is big.All sick trees are removed currently, relying primarily on to pine nematode to administer, but because
For the particularity of its cause of disease life cycle, effect is unobvious.Therefore, be suitable for from Infection cycie and diffusion mechanism set off in search is anti-
Method is controlled as the task of top priority.
The cause of disease of pine nematode is Bursaphelenchus xylophilus, is one of most important Alien invasive species in China.It can not itself be complete
At Spreading and diffusion task, the Spreading and diffusion between pine tree must be completed by the carrying of insect vector Monochamus alternatus.Woodland
The first disease time of pine nematode generally at the beginning of 6 months, 7, August part be pine nematode onset peak.It is invaded by Bursaphelenchus xylophilus
The pine tree contaminated is the place that Monochamus alternatus likes oviposition.The month in coming year 1-5, Bursaphelenchus xylophilus enter diapause, form diffused pine
Nematode.Husking forms diffused third-instar larvae (L firstⅢ), and be gathered in around Monochamus alternatus pupa room, June is with Monochamus alternatus
Emergence and form diffused four-age larva (LⅣ) and be carried into Monochamus alternatus stomata and travel to new healthy pine tree, start
The Infection cycie of a new round.LⅣEnter resin canal by wound caused on bark when Monochamus alternatus Hope feeding or oviposition, so
Husking forms adult afterwards, lays eggs after these female, male imago matings, become reproduction type pine wood nematode and mass propagation after egg hatching and
Making pine tree, withered turn yellow becomes the object of Monochamus alternatus oviposition again quickly, and the alternatus Larva of spawning and hatching enters pine again
Feeding burrow in nematode sick tree forms pupa room.The two cooperation provides required condition for other side, and utilize the completion history of life
The condition that other side creates completes itself development.In short, diffused four-age larva (LⅣ) formed time and Monochamus alternatus sprout wings when
Between to agree be the key that Bursaphelenchus xylophilus is carried propagation.Stop to the diffusive transport of Bursaphelenchus xylophilus is prevention and control Bursaphelenchus xylophilus
Key.Therefore, the development of alternatus Larva is interfered and is regulated and controled, Monochamus alternatus emergence time can be postponed or
It can not sprout wings, to make LⅣIt can not form and cut off the approach of its Spreading and diffusion.
Summary of the invention
In order to realize the obstruction to Monochamus alternatus prevention and treatment and pine nematode diffusive transport, the present invention provides a kind of new prevention and control
Method.
Technical scheme is as follows:
Ascarid glucoside △ C9 is used to Monochamus alternatus prevention and treatment and stops Bursaphelenchus xylophilus propagate by the present invention.
The method provided by the present invention for ascarid glucoside △ C9 being used to prevent and treat Monochamus alternatus and stop Bursaphelenchus xylophilus propagation are as follows: by ascarid
Glucoside △ C9 is placed in the pine tree containing alternatus Larva.
It is provided by the present invention that ascarid glucoside △ C9 is used for Monochamus alternatus prevention and treatment and stops the side that Bursaphelenchus xylophilus is propagated as optimization
Method are as follows: ascarid glucoside △ C9 is adsorbed in the carrier for having adsorptivity and slow release effect, which is placed in alternatus Larva
In pine tree.The carrier for having adsorptivity and slow release effect is silicon rubber chock plug.
It is provided by the present invention that ascarid glucoside △ C9 is used for Monochamus alternatus prevention and treatment and stops the side that Bursaphelenchus xylophilus is propagated as optimization
Method are as follows:
1), ascarid glucoside △ C9 and organic solvent are mixed, obtain mixed liquor；
2), 20 μ l of mixed liquor obtained by step 1) is contacted with the carrier for having adsorptivity and slow release effect, to organic solvent
After volatilization, which is placed in the pine tree containing alternatus Larva.
The organic solvent is selected from methanol, ethyl alcohol or ether.
It is provided by the present invention that ascarid glucoside △ C9 is used for Monochamus alternatus prevention and treatment and stops the side that Bursaphelenchus xylophilus is propagated as optimization
In method, the dosage of ascarid glucoside △ C9 are as follows: 150nM-150 μM/m3Pine tree body.
What the present invention was realized has the beneficial effect that:
Ascarid glucoside △ C9 of the present invention is artificial sintetics, and stable structure, chemical property is steady under normal temperature and pressure
It is fixed, it is nontoxic, it is free from environmental pollution.
It is provided by the present invention to be used to Monochamus alternatus prevention and treatment by ascarid glucoside △ C9 and stop the method that Bursaphelenchus xylophilus is propagated operate
Simply, application easy to spread.
It is provided by the present invention that ascarid glucoside △ C9 is used for Monochamus alternatus prevention and treatment and stops the method for Bursaphelenchus xylophilus propagation to pine
Black longicorn prevention and treatment and the control efficiency for stopping Bursaphelenchus xylophilus propagation are obvious.
Specific embodiment
Ascarid glucoside △ C9 of the present invention can chemical synthesis, come from Tianjin Yaoming Kangde new drugs development Co., Ltd, English
Literary fame ascarosides (asc- △ C9).Molecular formula: C15H26O6.Its stable structure, chemical property is stablized under normal temperature and pressure, nothing
Poison, it is free from environmental pollution.Structural formula is as follows:Synthetic route is shown in document:
Ascaroside activity in Caenorhabditis elegans is highly dependent on chemical
Structure.Bioorganic&Medicinal Chemistry (2013) 21:5754-5769.
Silicon rubber chock plug is purchased from Pherobio Technology Co., Ltd., the limited public affairs of Beijing source Ge Ruibi science and technology
Department etc..
Embodiment 1:
In Bursaphelenchus xylophilus generating region, Monochamus alternatus at the beginning of 5 months by the end of April is pupated before beginning, selects 40 plants to contain multiple Song Motian
The masson pine of ox larva.Recognition methods are as follows: not resin flux after the pine tree drilling for having Bursaphelenchus xylophilus to endanger has Monochamus alternatus oviposition cut mark
Trace, part pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, in tree body sunny slope xylem Song Motian
Ox eats into the hole that 1cm diameter 2cm depth is bored into hole electric drill, communicates with alternatus Larva pupa room.To being directly added into experimental group hole
The hole ascarid glucoside △ C9 dosage 6nM/, ascarid glucoside △ C9 dosage are 30nM/m3Pine tree body.Control group does not add ascarid glucoside △ C9 and cuts open after 10 days
Alternatus Larva is observed out.The results show that the Monochamus alternatus of control group 83% is pupa, and experimental group 45% is pupa,
55% is larva.
Embodiment 2:
Ascarid glucoside △ C9 is dissolved in methanol, is adsorbed in silicon rubber chock plug (rubber septa) (0.5cm diameter, 1cm long).?
Bursaphelenchus xylophilus generating region selects 40 plants of masson pines for containing multiple alternatus Larvas.Recognition methods are as follows: have Bursaphelenchus xylophilus harm
Pine tree drilling after not resin flux, have Monochamus alternatus oviposition cut mark trace, part pine needle chlorosis yellow.20 plants be used as experimental group, 20
Strain as a control group, the hole that 1cm diameter 2cm depth is bored into hole electric drill is eaten into tree body sunny slope xylem Monochamus alternatus.Experimental group
Several dispersions of the silicon rubber chock plug of 1 μ l of the △ of glucoside containing ascarid C9 are inserted in the hole (after 5 μ l ascarid glucoside △ C9 and 100 μ l methanol are mixed,
Take 20 μ l to instill silicon rubber chock plug, can be used after solvent volatilization), ascarid glucoside △ C9 dosage is 5 μ l/m3Pine tree body.Control group insertion
The silicon rubber chock plug of the 1 μ l containing methanol, after 10 days, cuts alternatus Larva and is observed.The results show that the pine of control group 81%
Black longicorn is pupa, and experimental group 41% is pupa, and 59% is larva.
Embodiment 3:
Ascarid glucoside △ C9 is dissolved in ethyl alcohol, is adsorbed in silicon rubber chock plug (0.5cm diameter, 1cm long).Occur in Bursaphelenchus xylophilus
Area selects 40 plants of masson pines for containing multiple alternatus Larvas.Recognition methods are as follows: after the pine tree drilling for thering is Bursaphelenchus xylophilus to endanger
Not resin flux has Monochamus alternatus oviposition cut mark trace, part pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants are as a control group,
The hole that 1cm diameter 2cm depth is bored into hole electric drill is eaten into tree body sunny slope xylem Monochamus alternatus.Experimental group is by the △ of glucoside containing ascarid C9's
Several dispersions of silicon rubber chock plug, which insert in the hole, (takes 20 μ l to instill silicon rubber chock plug 3 μM of ascarid glucoside △ C9 ethanols, after solvent volatilization
Can be used), ascarid glucoside △ C9 dosage is 15 μM/m3Pine tree body.Control group is inserted into the silicon rubber chock plug of the 20 μ l containing ethyl alcohol, after 10 days,
Alternatus Larva is cut to be observed.The results show that the Monochamus alternatus of control group 84% is pupa, and experimental group 23% develops into
Pupa, 77% is larva.
Embodiment 4:
Ascarid glucoside △ C9 is dissolved in ether, is adsorbed in silicon rubber chock plug (0.5cm diameter, 1cm long).Occur in Bursaphelenchus xylophilus
Area selects 40 plants of masson pines for containing multiple alternatus Larvas.Recognition methods are as follows: after the pine tree drilling for thering is Bursaphelenchus xylophilus to endanger
Not resin flux has Monochamus alternatus oviposition cut mark trace, part pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants are as a control group,
The hole that 1cm diameter 2cm depth is bored into hole electric drill is eaten into tree body sunny slope xylem Monochamus alternatus.Experimental group is by the △ of glucoside containing ascarid C9's
Several dispersions of silicon rubber chock plug, which insert in the hole, (to be taken 20 μ l to instill silicon rubber chock plug 3 μM of ascarid glucoside △ C9 ether solutions, 20 μ l is taken to instill
Silicon rubber chock plug can be used after solvent volatilization), ascarid glucoside △ C9 dosage is 15 μM/m3Pine tree body.Control group is inserted into the 20 μ l containing ether
Silicon rubber chock plug, after 10 days, cut alternatus Larva and observed.The results show that the Monochamus alternatus of control group 78% is
Pupa, and experimental group 34% develops pupa, 66% is larva.
Embodiment 5:
Ascarid glucoside △ C9 is dissolved in ethyl alcohol, is adsorbed in cotton (0.5cm diameter, 1cm long).In Bursaphelenchus xylophilus generating region, selection
40 plants of masson pines containing multiple alternatus Larvas.Recognition methods are as follows: not resin flux after the pine tree drilling for thering is Bursaphelenchus xylophilus to endanger,
There are Monochamus alternatus oviposition cut mark trace, part pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, in tree body
Sunny slope xylem Monochamus alternatus eats into the hole that 1cm diameter 2cm depth is bored into hole electric drill.If experimental group is by the cotton of the △ of glucoside containing ascarid C9
Dry dispersion inserts in the hole and (takes 20 μ l to instill cotton 3 μM of ascarid glucoside △ C9 ethanols, to can be used after solvent volatilization), and ascarid glucoside △ C9 is used
Amount is 15 μM/m3Pine tree body.Control group is inserted into the cotton of the 20 μ l containing methanol, after 10 days, cuts alternatus Larva and is observed.
The results show that the Monochamus alternatus of experimental group 21% is pupa, and control group 80% develops pupa, and 20% is larva.
Embodiment 6:
Ascarid glucoside △ C9 is adsorbed in charcoal (0.5cm diameter, 1cm long).In Bursaphelenchus xylophilus generating region, 40 plants of selection containing more
The masson pine of a alternatus Larva.Recognition methods are as follows: not resin flux after the pine tree drilling for having Bursaphelenchus xylophilus to endanger has Monochamus alternatus
Oviposition cut mark trace, part pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, wooden in tree body sunny slope
Portion's Monochamus alternatus eats into the hole that 1cm diameter 2cm depth is bored into hole electric drill.Several dispersions of the charcoal of the △ of glucoside containing ascarid C9 are inserted by experimental group
In hole (by 3 μM of ascarid glucoside △ C9 ethanols take 20 μ l instill charcoal, solvent volatilization after can be used), ascarid glucoside △ C9 dosage be 15 μM/
m3Pine tree body.Control group is inserted into the charcoal of the 20 μ l containing ethyl alcohol, after 10 days, cuts alternatus Larva and is observed.The results show that
The Monochamus alternatus of experimental group 43% is pupa, and control group 78% develops pupa, and 22% is larva.
Embodiment 7:
By ascarid glucoside △ C9 dose change.In Bursaphelenchus xylophilus generating region, 40 plants of horses for containing multiple alternatus Larvas are selected
Tail pine.Recognition methods are as follows: not resin flux after the pine tree drilling for having Bursaphelenchus xylophilus to endanger has Monochamus alternatus oviposition cut mark trace, part
Pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, eat into tree body sunny slope xylem Monochamus alternatus and use into hole
The hole of electric drill brill 1cm diameter 2cm depth.Experimental group inserts in the hole several dispersions of the silicon rubber chock plug of 20 μ l of the △ of glucoside containing ascarid C9 (will
3 μM of ascarid glucoside △ C9 ethanols, take 20 μ l instill silicon rubber chock plug, solvent volatilization after can be used), ascarid glucoside △ C9 dosage be 15 μM/
m3Pine tree body.Control group is inserted into the silicon rubber chock plug of the 20 μ l containing ethyl alcohol, after 10 days, cuts alternatus Larva and is observed.Knot
Fruit shows that the Monochamus alternatus of experimental group 44% is pupa, and control group 80% develops pupa, and 20% is larva.
Embodiment 8:
By ascarid glucoside △ C9 dose change.In Bursaphelenchus xylophilus generating region, 40 plants of horses for containing multiple alternatus Larvas are selected
Tail pine.Recognition methods are as follows: not resin flux after the pine tree drilling for having Bursaphelenchus xylophilus to endanger has Monochamus alternatus oviposition cut mark trace, part
Pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, eat into tree body sunny slope xylem Monochamus alternatus and use into hole
The hole of electric drill brill 1cm diameter 2cm depth.Experimental group inserts in the hole several dispersions of the silicon rubber chock plug of 20 μ l of the △ of glucoside containing ascarid C9 (will
3 μM of ascarid glucoside △ C9 ethanols, take 20 μ l instill silicon rubber chock plug, solvent volatilization after can be used), ascarid glucoside △ C9 dosage be 150 μM/
m3Pine tree body.Control group is inserted into the silicon rubber chock plug of the 20 μ l containing ethyl alcohol, after 10 days, cuts alternatus Larva and is observed.Knot
Fruit shows that the Monochamus alternatus of experimental group 36% is pupa, and control group 78% develops pupa, and 22% is larva.
Embodiment 9:
By ascarid glucoside △ C9 dose change.In Bursaphelenchus xylophilus generating region, 40 plants of horses for containing multiple alternatus Larvas are selected
Tail pine.Recognition methods are as follows: not resin flux after the pine tree drilling for having Bursaphelenchus xylophilus to endanger has Monochamus alternatus oviposition cut mark trace, part
Pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, eat into tree body sunny slope xylem Monochamus alternatus and use into hole
The hole of electric drill brill 1cm diameter 2cm depth.Experimental group inserts in the hole several dispersions of the silicon rubber chock plug of 20 μ l of the △ of glucoside containing ascarid C9 (will
300nM ascarid glucoside △ C9 ethanol takes 20 μ l to instill silicon rubber chock plug, can be used after solvent volatilization), ascarid glucoside △ C9 dosage is 1.5 μ
M/m3Pine tree body.Control group is inserted into the silicon rubber chock plug of the 20 μ l containing ethyl alcohol, after 10 days, cuts alternatus Larva and is observed.
The results show that the Monochamus alternatus of experimental group 32% is pupa, and control group 81% develops pupa, and 19% is larva.
Embodiment 10:
By ascarid glucoside △ C9 dose change.In Bursaphelenchus xylophilus generating region, 40 plants of horses for containing multiple alternatus Larvas are selected
Tail pine.Recognition methods are as follows: not resin flux after the pine tree drilling for having Bursaphelenchus xylophilus to endanger has Monochamus alternatus oviposition cut mark trace, part
Pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, eat into tree body sunny slope xylem Monochamus alternatus and use into hole
The hole of electric drill brill 1cm diameter 2cm depth.Experimental group inserts in the hole several dispersions of the silicon rubber chock plug of 20 μ l of the △ of glucoside containing ascarid C9 (will
30nM ascarid glucoside △ C9 ethanol, take 20 μ l to instill silicon rubber chock plug, can be used after solvent volatilization), ascarid glucoside △ C9 dosage is
150nM/m3Pine tree body.Control group is inserted into the silicon rubber chock plug of the 20 μ l containing ethyl alcohol, after 10 days, cuts alternatus Larva and is seen
It examines.The results show that the Monochamus alternatus of experimental group 38% is larva, and control group 82% develops pupa, and 18% is larva.
Embodiment 11:
By ascarid glucoside △ C9 dose change.In Bursaphelenchus xylophilus generating region, 40 plants of horses for containing multiple alternatus Larvas are selected
Tail pine.Recognition methods are as follows: not resin flux after the pine tree drilling for having Bursaphelenchus xylophilus to endanger has Monochamus alternatus oviposition cut mark trace, part
Pine needle chlorosis yellow.20 plants are used as experimental group, and 20 plants as a control group, eat into tree body sunny slope xylem Monochamus alternatus and use into hole
The hole of electric drill brill 1cm diameter 2cm depth.Experimental group inserts in the hole several dispersions of the silicon rubber chock plug of 20 μ l of the △ of glucoside containing ascarid C9 (will
3nM ascarid glucoside △ C9 ethanol takes 20 μ l to instill silicon rubber chock plug, can be used after solvent volatilization), ascarid glucoside △ C9 dosage is 15nM/
m3Pine tree body.Control group is inserted into the silicon rubber chock plug of the 20 μ l containing ethyl alcohol, after 10 days, cuts alternatus Larva and is observed.Knot
Fruit shows that the Monochamus alternatus of experimental group 44% is pupa, and control group 79% develops pupa, and 21% is larva.
Under field conditions (factors), diffused Bursaphelenchus xylophilus be largely formed in the 1-5 month in the coming year, and with insect vector pine
Black longicorn emergence time is consistent, can be carried by Monochamus alternatus and travel to new healthy pine tree.It is mentioned by using the present invention
The method of confession can make alternatus Larva stop hair at the beginning of annual May to 6 months during July, especially just to fall ill
It educates, reduces Bursaphelenchus xylophilus pupa and adult population density, reduce pine death rate, meanwhile, make Bursaphelenchus xylophilus can not be with Song Motian
The emergence of ox and form diffused four ages nematode, and stop its diffusive transport, and then reach interference diffusion is propagated further in it
Target reduces Bursaphelenchus xylophilus generating region pine death rate and diffusion rate of propagation to realize, and prevents further climing in forest zone
Prolong the prevention and control target of extension.
Method provided by the invention is administered using the pine tree for being infected Bursaphelenchus xylophilus in woodland.It can solve in pine line
The problem of Bursaphelenchus xylophilus mass propagation of worm epidemic situation spot occurs and spreads rapidly.
Claims (7)
1. ascarid glucoside △ C9 is in prevention and treatment Monochamus alternatus and stops the application in Bursaphelenchus xylophilus propagation.
2. the method for being used to prevent and treat Monochamus alternatus for ascarid glucoside △ C9 and stopping Bursaphelenchus xylophilus propagation: ascarid glucoside △ C9 is placed in containing pine
In the pine tree of black roundheaded borer.
3. the method for being used to prevent and treat Monochamus alternatus for ascarid glucoside △ C9 described in claim 2 and stopping Bursaphelenchus xylophilus propagation, feature
It is, ascarid glucoside △ C9 is adsorbed in the carrier for having adsorptivity and slow release effect, which is placed in containing Monochamus alternatus children
In the pine tree of worm.
4. the method for being used to prevent and treat Monochamus alternatus for ascarid glucoside △ C9 described in claim 3 and stopping Bursaphelenchus xylophilus propagation, feature
It is, the carrier for having adsorptivity and slow release effect is silicon rubber chock plug.
5. the method for being used to prevent and treat Monochamus alternatus for ascarid glucoside △ C9 described in claim 3 and stopping Bursaphelenchus xylophilus propagation, feature
It is, steps are as follows:
1) ascarid glucoside △ C9 is mixed with organic solvent, obtains mixed liquor；
2), mixed liquor obtained by step 1) is contacted with the carrier for having adsorptivity and slow release effect, it, will after organic solvent volatilization
The carrier is placed in the pine tree containing alternatus Larva.
6. the method for being used to prevent and treat Monochamus alternatus for ascarid glucoside △ C9 described in claim 5 and stopping Bursaphelenchus xylophilus propagation, feature
It is, the organic solvent is selected from methanol, ethyl alcohol or ether.
7. being used to prevent and treat Monochamus alternatus for ascarid glucoside △ C9 described in claim 2-6 any one and stopping the side of Bursaphelenchus xylophilus propagation
Method, which is characterized in that the dosage of ascarid glucoside △ C9 are as follows: 150nM-150 μM/m3Pine tree body.
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| TWI401028B (en) * | 2006-12-27 | 2013-07-11 | Ishihara Sangyo Kaisha | Control the composition of tree parasitic pests |
| US8318146B1 (en) * | 2008-02-01 | 2012-11-27 | The United States Of America, As Represented By The Secretary Of Agriculture | Ascarosides as nematode sex pheromones |
| CN101564051A (en) * | 2009-04-14 | 2009-10-28 | 云南农业大学 | Monochamus alternatus imago attractant |
| CN101904342A (en) * | 2010-07-28 | 2010-12-08 | 惠州市神龙松材线虫病综合治理有限公司 | Monochamus alternatus positioning type attractant and laying type attractant thereof |
| CN102027901B (en) * | 2010-12-07 | 2013-05-01 | 浙江农林大学 | Adult monochamus alternatus hope attractant |
| EP2741752A4 (en) * | 2011-08-08 | 2015-04-01 | California Inst Of Techn | The utility of nematode small molecules |
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