CN106333985A - Method for preparing Rhodiola rosea effective part - Google Patents

Method for preparing Rhodiola rosea effective part Download PDF

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Publication number
CN106333985A
CN106333985A CN201610611440.XA CN201610611440A CN106333985A CN 106333985 A CN106333985 A CN 106333985A CN 201610611440 A CN201610611440 A CN 201610611440A CN 106333985 A CN106333985 A CN 106333985A
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Prior art keywords
rhizome
rhodiola root
water
extract
active component
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Chinese (zh)
Inventor
车洪泽
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Chengdu Winona Biological Science And Technology Co Ltd
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Chengdu Winona Biological Science And Technology Co Ltd
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Priority to CN201610611440.XA priority Critical patent/CN106333985A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/41—Crassulaceae (Stonecrop family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses a method for preparing a Rhodiola rosea effective part. The method comprises the following steps: 1, peeling dry rhizomes of a Rhodiola plant to obtain stems and cortices, slicing the Rhodiola rosea stems, crushing the sliced Rhodiola rosea stems, and extracting the crushed Rhodiola rosea stems through methanol or ethanol in order to obtain a rhizome extract liquid; 2, carrying out reduced pressure treatment on the rhizome extract liquid to recover a solvent in order to obtain a rhizome extract paste; 3, adjusting the rhizome extract paste to a proper density, carrying out water precipitation in pure water, filtering obtained precipitate and water, and carrying out reduced pressure concentration on the above obtained filtrate to obtain a Rhodiola rosea rhizome total extract product; and 4, separating the Rhodiola rosea rhizome total extract product through using macro-porous adsorption resin, carrying out gradient elution by using a water and ethanol system, carrying out reduced pressure concentration on the above obtained rhizome eluate, and freeze-drying the obtained concentrate to obtain the Rhodiola rosea rhizome effective part. The method for preparing the Rhodiola rosea effective part through a peel and stem separation process, an extraction process and a water precipitation process has the characteristics of simplicity, good practicality, and high preparation efficiency.

Description

The method preparing rhodiola root active component
Technical field
The present invention relates to natural products fabricating technology field, specifically, it is the side preparing rhodiola root active component Method.
Background technology
Rhodiola, in Rosales Crassulaceae (crassulaceae) Rhodida plant, is perennial herb or sub- filling Wood, is distributed in the areas such as China Tibet, Yunnan-Guizhou, northeast, Gansu, Xinjiang, Sichuan, containing rhodioside, flavones, amino acid, junket Alcohol, trace element, cumarin etc. more than 40 plant compound, anti anoxia, antifatigue, antiviral, strengthen immunity of organisms, anti-ageing Always, the aspect such as radioresistance, bidirectional modulation body function has remarkable efficacy.Wherein rhodioloside is the main medicine of rhodiola plant Reason active component, has extensive demand and using value in industries such as medicine, cosmetics and health foods.
Nagqu Diqu, the Shannan Prefecture, height above sea level all in more than 4000m, has the characteristic of awful weather, is produced natural rhodiola root There is the high characteristic of medical value.
Content of the invention
It is an object of the invention to provide the method preparing rhodiola root active component, by skin bar separating technology, extraction work Skill, water sink technique etc. and carry out the preparation of rhodiola root active component, have features simple and practical process, the features such as preparation efficiency is high.
The present invention is achieved through the following technical solutions: the method preparing rhodiola root active component, comprises the following steps:
1) by the dry rhizome peeling of Rhodida plant, obtain stem and skin zone, pulverize after rhodiola root stem is cut into slices, pulverizing Rhodiola root rhizome is extracted by methyl alcohol or ethanol, obtains rhizome extract；
2) by rhizome extract decompression and solvent recovery, obtain rhizome medicinal extract；
3) rhizome medicinal extract is adjusted to suitable density, pure water carries out water and sinks, filter, filtrate reduced in volume, obtain root of Rhodiola sachalinensis Stem total extract；
4) rhodiola root rhizome total extract is carried out with macroporous absorbent resin separating, carry out gradient elution with water, ethanol system, will Rhizome eluent reduced pressure concentration, obtains rhodiola root rhizome active component after freeze-drying；
5) rhodiola root skin zone is pulverized, the rhodiola root skin zone of pulverizing is extracted by acetone, obtains skin zone's extract；
6) by skin zone's extract decompression and solvent recovery, obtain skin zone's medicinal extract；
7) skin zone's medicinal extract is adjusted to proper density, pure water carries out water and sinks, filter, filtrate reduced in volume, obtain rhodiola root skin Portion's total extract；
8) rhodiola root skin zone total extract is carried out with macroporous absorbent resin separating, carry out gradient elution with water, skin zone is eluted Liquid reduced pressure concentration, freeze-drying Hou get rhodiola root skin zone active component.
Further to better implement the present invention, especially adopt following set-up modes: in described step 1), methyl alcohol or second Alcohol adopts the methyl alcohol that concentration is 45% ~ 90% or ethanol, and consumption is 8 ~ 10 times of rhodiola root rhizome weight, and extraction process is 3 ~ 4 times, Extraction time is 3.5 ~ 4.5 hours every time.
Further to better implement the present invention, especially adopt following set-up modes: described suitable density is 1.20 ~ 1.25；And in step 3), the heavy step of water stands 36 ~ 64 hours at 11 ~ 14 DEG C, when water sinks, institute's water requirement is rhizome medicinal extract 5 ~ 8 times of weight.
Further to better implement the present invention, especially adopt following set-up modes: in described step 5), acetone dense Spend for 60% ~ 80% acetone solution, consumption is 7 ~ 8 times of rhodiola root rhizome weight, extraction process is 3 ~ 4 times, each extraction time is 3 ~ 4 hours.
Further to better implement the present invention, especially adopt following set-up modes: described proper density is 1.10 ~ 1.14；And in step 7), the heavy step of water stands 36 ~ 64 hours at 8 ~ 9 DEG C, when water sinks, institute's water requirement is rhizome medicinal extract weight 6 ~ 8 times of amount.
Further to better implement the present invention, following set-up modes are especially adopted: described macroporous absorbent resin is weak Basic macroporous resin or Microporous resin.
Further to better implement the present invention, following set-up modes are especially adopted: described Rhodida plant adopts Height above sea level is in the rhodiola root of more than 4000m.
The present invention compared with prior art, has advantages below and a beneficial effect:
The present invention passes through the preparation that skin bar separating technology, extracting technology, the heavy technique of water etc. carry out rhodiola root active component, has work Skill is simple and practical, the features such as preparation efficiency is high.
The present invention is enriched with to gadol extract by macroreticular resin, eliminates substantial amounts of carbohydrate content and other Some impurity components, greatly reduce the dosage of use, and the active component preparing gained is only the 0.5% ~ 0.8% of crude drug in whole, has It is prepared into the Chinese patent drug of various formulations beneficial to exploitation further.
Gadol extract provided by the present invention has preparation process is simple, easy to operate, low production cost, safe and reliable The advantages of, whole process meets the requirement of environmental protection.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this.
Embodiment 1:
The method preparing rhodiola root active component, carrying out rhodiola root by skin bar separating technology, extracting technology, the heavy technique of water etc. has The preparation at effect position, has features simple and practical process, the features such as preparation efficiency is high, especially adopts following set-up modes: include following Step:
1) by the dry rhizome peeling of Rhodida plant, obtain stem and skin zone, pulverize after rhodiola root stem is cut into slices, pulverizing Rhodiola root rhizome is extracted by methyl alcohol or ethanol, obtains rhizome extract；
2) by rhizome extract decompression and solvent recovery, obtain rhizome medicinal extract；
3) rhizome medicinal extract is adjusted to suitable density, pure water carries out water and sinks, filter, filtrate reduced in volume, obtain root of Rhodiola sachalinensis Stem total extract；
4) rhodiola root rhizome total extract is carried out with macroporous absorbent resin separating, carry out gradient elution with water, ethanol system, will Rhizome eluent reduced pressure concentration, obtains rhodiola root rhizome active component after freeze-drying；
5) rhodiola root skin zone is pulverized, the rhodiola root skin zone of pulverizing is extracted by acetone, obtains skin zone's extract；
6) by skin zone's extract decompression and solvent recovery, obtain skin zone's medicinal extract；
7) skin zone's medicinal extract is adjusted to proper density, pure water carries out water and sinks, filter, filtrate reduced in volume, obtain rhodiola root skin Portion's total extract；
8) rhodiola root skin zone total extract is carried out with macroporous absorbent resin separating, carry out gradient elution with water, skin zone is eluted Liquid reduced pressure concentration, freeze-drying Hou get rhodiola root skin zone active component.
Embodiment 2:
The present embodiment is to optimize further on the basis of above-described embodiment, further to better implement the present invention, especially Using following set-up modes: in described step 1), methyl alcohol or ethanol adopt the methyl alcohol that concentration is 45% ~ 90% or ethanol, and consumption is 8 ~ 10 times of rhodiola root rhizome weight, extraction process is 3 ~ 4 times, and each extraction time is 3.5 ~ 4.5 hours.
Embodiment 3:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, further to better implement the present invention, Especially adopt following set-up modes: described suitable density is 1.20 ~ 1.25；And in step 3), water sinks step at 11 ~ 14 DEG C Standing 36 ~ 64 hours, when water sinks, institute's water requirement is 5 ~ 8 times of rhizome medicinal extract weight.
Embodiment 4:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, further to better implement the present invention, Especially adopt following set-up modes: in described step 5), the concentration of acetone is 60% ~ 80% acetone solution, and consumption is rhodiola root rhizome 7 ~ 8 times of weight, extraction process is 3 ~ 4 times, and each extraction time is 3 ~ 4 hours.
Embodiment 5:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, further to better implement the present invention, Especially adopt following set-up modes: described proper density is 1.10 ~ 1.14；And in step 7), the heavy step of water is quiet at 8 ~ 9 DEG C Put 36 ~ 64 hours, when water sinks, institute's water requirement is 6 ~ 8 times of rhizome medicinal extract weight.
Embodiment 6:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, further to better implement the present invention, Especially adopt following set-up modes: described macroporous absorbent resin is alkalescent macroreticular resin or Microporous resin.
Embodiment 7:
The present embodiment is to optimize further on the basis of any of the above-described embodiment, further to better implement the present invention, Especially adopt following set-up modes: described Rhodida plant adopts height above sea level in the rhodiola root of more than 4000m.
The above, be only presently preferred embodiments of the present invention, and not the present invention is done with any pro forma restriction, every according to Any simple modification above example made according to the technical spirit of the present invention, equivalent variations, each fall within the protection of the present invention Within the scope of.

Claims (7)

1. prepare the method for rhodiola root active component it is characterised in that: comprise the following steps:
1) by the dry rhizome peeling of Rhodida plant, obtain stem and skin zone, pulverize after rhodiola root stem is cut into slices, pulverizing Rhodiola root rhizome is extracted by methyl alcohol or ethanol, obtains rhizome extract；
2) by rhizome extract decompression and solvent recovery, obtain rhizome medicinal extract；
3) rhizome medicinal extract is adjusted to suitable density, pure water carries out water and sinks, filter, filtrate reduced in volume, obtain root of Rhodiola sachalinensis Stem total extract；
4) rhodiola root rhizome total extract is carried out with macroporous absorbent resin separating, carry out gradient elution with water, ethanol system, will Rhizome eluent reduced pressure concentration, obtains rhodiola root rhizome active component after freeze-drying；
5) rhodiola root skin zone is pulverized, the rhodiola root skin zone of pulverizing is extracted by acetone, obtains skin zone's extract；
6) by skin zone's extract decompression and solvent recovery, obtain skin zone's medicinal extract；
7) skin zone's medicinal extract is adjusted to proper density, pure water carries out water and sinks, filter, filtrate reduced in volume, obtain rhodiola root skin Portion's total extract；
8) rhodiola root skin zone total extract is carried out with macroporous absorbent resin separating, carry out gradient elution with water, skin zone is eluted Liquid reduced pressure concentration, freeze-drying Hou get rhodiola root skin zone active component.
2. the method preparing rhodiola root active component according to claim 1 it is characterised in that: in described step 1), first Alcohol or ethanol adopt the methyl alcohol that concentration is 45% ~ 90% or ethanol, and consumption is 8 ~ 10 times of rhodiola root rhizome weight, and extraction process is 3 ~ 4 times, each extraction time is 3.5 ~ 4.5 hours.
3. the method preparing rhodiola root active component according to claim 1 it is characterised in that: described suitable density is 1.20~1.25；And in step 3), the heavy step of water stands 36 ~ 64 hours at 11 ~ 14 DEG C, when water sinks, institute's water requirement is rhizome 5 ~ 8 times of medicinal extract weight.
4. the method preparing rhodiola root active component according to claim 1 it is characterised in that: in described step 5), third The concentration of ketone is 60% ~ 80% acetone solution, and consumption is 7 ~ 8 times of rhodiola root rhizome weight, and extraction process is 3 ~ 4 times, extracts every time Time is 3 ~ 4 hours.
5. the method preparing rhodiola root active component according to claim 1 it is characterised in that: described proper density is 1.10~1.14；And in step 7), the heavy step of water stands 36 ~ 64 hours at 8 ~ 9 DEG C, when water sinks, institute's water requirement is that rhizome soaks 6 ~ 8 times of cream weight.
6. the method preparing rhodiola root active component according to claim 1 it is characterised in that: described macroporous absorbent resin For alkalescent macroreticular resin or Microporous resin.
7. the method preparing rhodiola root active component according to claim 1 it is characterised in that: described Rhodida plant Using height above sea level more than 4000m rhodiola root.
CN201610611440.XA 2016-07-30 2016-07-30 Method for preparing Rhodiola rosea effective part Pending CN106333985A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112973173A (en) * 2019-12-18 2021-06-18 中国科学院地理科学与资源研究所 Resource utilization method of super-enriched plant biomass

Citations (2)

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Publication number Priority date Publication date Assignee Title
CN102526165A (en) * 2010-12-07 2012-07-04 中国医学科学院药物研究所 Rhodiola effective fractions, preparation method, drug composition and uses thereof
CN103054929A (en) * 2011-10-24 2013-04-24 中国医学科学院药物研究所 Rhodiola extract and pharmaceutical composition thereof as well as application of rhodiola extract in treatment of fatty liver disease

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN102526165A (en) * 2010-12-07 2012-07-04 中国医学科学院药物研究所 Rhodiola effective fractions, preparation method, drug composition and uses thereof
CN103054929A (en) * 2011-10-24 2013-04-24 中国医学科学院药物研究所 Rhodiola extract and pharmaceutical composition thereof as well as application of rhodiola extract in treatment of fatty liver disease

Non-Patent Citations (2)

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Title
杨晓艳等: "HPLC法测定红景天根皮中红景天苷的含量", 《分析试验室》 *
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112973173A (en) * 2019-12-18 2021-06-18 中国科学院地理科学与资源研究所 Resource utilization method of super-enriched plant biomass
CN112973173B (en) * 2019-12-18 2022-01-28 中国科学院地理科学与资源研究所 Resource utilization method of super-enriched plant biomass

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Application publication date: 20170118