CN106332870A - Acer palmatum thunb variety acer matsumurae green retention method - Google Patents
Acer palmatum thunb variety acer matsumurae green retention method Download PDFInfo
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- CN106332870A CN106332870A CN201610719558.4A CN201610719558A CN106332870A CN 106332870 A CN106332870 A CN 106332870A CN 201610719558 A CN201610719558 A CN 201610719558A CN 106332870 A CN106332870 A CN 106332870A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses an acer palmatum thunb variety acer matsumurae green retention method, and belongs to the technical field of plant specimen production. According to the present invention, Mg<2+> in the leaf is replaced with Cu<2+> so as to achieve the long-term green retention of the green leaf, such that the plant specimen is retained for a long time, the morphological structure of the material is preserved at a maximum, the specimen observation clarity is increased, the problems that the long-term green retention of the green plant specimen cannot be achieved and the environmental pollution exists during the specimen preservation and storage are solved, and the good application prospects are provided in the plant research and plant viewing fields.
Description
Technical field
The present invention relates to plant specimen preparation field, more particularly to a kind of preparation method of green plantss specimen, specifically
Be the protection method for green being related to a kind of yellow eight zhang of Acer palmatum kind.
Background technology
Yellow eight zhang (acer matsumurae " ki hachijoh "), Aceraceae, mountain maple class, deciduous tree.Single leaf opposite,
Tool pillow handle, petiole crosscutting mask ditch, blade is born in edge, mesophyll, blade length-width ratio 1:1.1.Palmate drastic crack, normal 7 split,
Sliver lanceolar, apart from 2mm, valve margin is inwardly rolled into semicircle to two slivers, has Myriopteron extensum (Wight) K. Schum, yellow green, raised, there is vein on surface.
Medium blade size, the light yellow green of young leaves, gradually switch to bottle green, summer is difficult to burn day, and autumn leaves golden yellow slightly carries rose-red,
It is subsequently changed to light orange and red secondary colour.All there is preferable ornamental value in spring and summer autumn.Summer bottle green blade and light green
Color fruit brings out the best in each other, and allows people still can feel its vitality in hot summer.It displays, shows and art in specimen
The aspect such as viewing and admiring also there is important value.
The complex chemical composition being contained within due to plant, its chemical change rule is difficult to grasp, and soaks system to primary colour plant
The making of specimen brings very big difficulty.At present, conventional plant Slide processing includes: exsiccata, dipping specimen, leaf
Arteries and veins preparation of specimen.Exsiccata manufacture method is simple and easy to operate, but specimen can not keep plant primary colors, and color leafed plants are protected
Deposit particularly disadvantageous；Dipping specimen needs to process using chemical drugss, intricate operation and Some Drugs are poisonous；Vein preparation of specimen work
Sequence is more complicated.To sum up, traditional plant primary colour specimen makes has following subject matter: one, poisonous using some more than preservation liquid
Reagent such as formalin, sulfurous acid etc., these materials are unstable, volatile, harmful；2nd, different fixatives are adopted
With the vegetable material preserving liquid preservation different colours, such as green plantss specimen fixative and preservation liquid, preparing solution wastes time and energy；
These traditional plant primary colour specimen infusion methods, need to prepare numerous fixatives and preserve liquid, workload is very big, simultaneously
Usually because of fixative and liquid preparation surplus can be preserved and bring very big waste；Traditional plant primary colour specimen infusion method application
Face is narrow, unrealistic and costly.
Content of the invention
For the deficiencies in the prior art, it is an object of the invention to provide one kind save time, laborsaving, simple to operate, protect green effect
Green plantss specimen preparation method really excellent, that guarantor's green time is lasting.
The preparation method of the green plantss specimen of the present invention, comprises the following steps:
(1) fresh, healthy, undamaged green plantss branch and leaf are gathered, cleaning and sterilizing obtains germ-free plant branch and leaf；
(2) prepare copper-bath, as fixative, fixative is heated, then the plant leaf of step (1) is put into solid
Determine in liquid, be changed into slowly becoming after green after yellowish-brown from green after plant leaf again and take out；
(3) plant specimen, compacting, bookbinding are rinsed, plastic packaging obtains final product.
Preferably, described green plantss are Acer palmatum.It is highly preferred that described Acer palmatum is the Acer palmatum of summer.Enter one
Step ground, described Acer palmatum is yellow eight zhang.
Preferably, the green branch and leaf of step (1) carry fruit.
In said method of the present invention, step (1) obtains germ-free plant by the way of 70% ethanol disinfection and clear water rinse
Branch and leaf.
Wherein, the compound method of the copper-bath of step (2) is, the volume ratio of glacial acetic acid, copper sulfate and water is 40:1:
100
In step (2), when fixative is heated to 80-90 DEG C, plant leaf is put in fixative.
Preferably, when fixing liquid temp is 85 DEG C, plant leaf is put in fixative.
The method of the present invention is to utilize cu2+Mg in displacement blade2+, so that green leaf piece protects green, not fugitive color for a long time,
Preserve the morphosiss of vegetable material to greatest extent, improve the definition that specimen is observed, preserve plant specimen beneficial to long-term, solve
Green plantss specimen cannot long Kubo be green and problem of environmental pollution during Saving specimen storage, in plant research, plant religion
Learn and plants ' aestheticses field has a good application prospect.
Brief description
Fig. 1 is that yellow eight zhang of summer trophophase leaf colors are shown.
Eight zhang of plastic packaging specimen of Huang that Fig. 2 is obtained for the inventive method, protect green excellent effect.
Specific embodiment
Unless otherwise defined, the implication that all technical term used hereinafter is generally understood that with those skilled in the art
Identical.Technical term used herein is intended merely to describe the purpose of specific embodiment, is not intended to limit the present invention
Protection domain.
Except there being special instruction, the various reagents used in the present invention, raw material be can commodity commercially or
The product that person can be obtained by known method.
The preparation method of the yellow eight zhang of specimen of embodiment 1
1st, picking blade
Collection fresh, healthy, undamaged summer eight zhang of branch and leaf specimen of Huang it is desirable to can not pluck rotten leaf, broken leaf, yellow leaf,
Sick leaf, blade, based on dark green, is preferably with fruit.
2nd, cleaning and disinfecting
Fresh specimens are cleaned silt, is then carried out disinfection with 70% ethanol clean more again with distilled water flushing after 5min
Put in distilled water leaching 15min, after rinse again 2-3 time, make blade surface clean clean till
3rd, specimen protects green process
(1) prepare mother solution and measure 200ml glacial acetic acid, add copper sulfate until saturation, then constant volume to make mother solution be 2000ml.
(2) decoct and be heated to about when 80～85 DEG C or micro-boiling putting into specimen by the mother solution preparing, observe its change, wait to mark
This is changed into after yellowish-brown slowly becoming taking-up after green from green again.
(3) plant specimen after rinsing is taken out puts in clear water and cleans, and dries.
4th, specimen compacting
Carry out specimen compacting: be 1. placed in the middle of the newspaper of folding by the specimen arranging after pruning, every part of specimen is placed and will be protected
Hold whole machine balancing, symmetrical, organ is complete, overall to unfold；2. so that stem, leaf is unfolded as far as possible；3. the newspaper pair of plant specimen will be carried secretly
Poststack covers 5～6 absorbent paper on upper strata, and when stacking, specimen notices that head and the tail are staggered, and makes stress during whole this fixation of mark compacting equal
Even, will not tilt.The specimen put in order the most at last is placed in specimen holder, is tied up with thickness rope made of hemp upper-lower compacting；4. newly adopt specimen
Compacting 4～5h must change dry newspaper, carries out shaping again to specimen, to identify and to show when changing toilet paper.
5th, specimen bookbinding
1. enamel tray bottom is smoothened adhesive (white glue with vinyl: water=3:7), take specimen to be stained with even adhesive；2. specimen is glued
The appropriate location on platform paper, reserves the lower right corner and the upper left corner and sticks field record label respectively, names label.By institute during stickup
There is blade suitably to allocate, make blade have tow sides, the organ that specimen falls down, collect and load paper bag and be attached to platform paper subscript
This origin-location；3. cement the part easy to fall off needlework such as stem, leaf of rear specimen to fix again, back side the end of a thread blank sheet of paper on platform paper
Paste, prevent mutual hook between specimen；4. the clip for clamping papers of specimen will be stained with newspaper, accumulate a dozen and put into specimen holder binding the moon
Dry, take out after specimen 6h after bookbinding.After the completion of plastic packaging obtain final product.
The impact of the specimen that embodiment 2 temperature and humidity is obtained to the present invention
The specimen that embodiment 1 method is obtained is positioned over 37 DEG C of temperature, and the climatic chamber of humidity 50%～60% preserves,
Observing time is 2 years.Result of study shows: in 2 years storage lives, specimen is always maintained at emerald green, and no colour fading, mould realization
As the morphosiss of yellow eight zhang of materials are clear and legible, have the advantages that safety, environmental protection.
The impact of the specimen that embodiment 3 illumination is obtained to the present invention
It is little for strong illumination 30 under conditions of 4500lx ± 500lx that the specimen that embodiment 1 method is obtained is positioned over illumination
When, observed within latter 0 day, 10 days, 20 days, 30 days, 40 days, 60 days in irradiating, found eight zhang of specimen one of Huang that embodiment 1 is obtained
Straight holding emerald green, and no colour fading, mildew phenomena, the morphosiss of vegetable material are clear and legible.
Above embodiment is only that the preferred embodiment of the present invention is described, and not the scope of the present invention is entered
Row limits, on the premise of without departing from design spirit of the present invention, the technical side to the present invention for this area ordinary skill technical staff
Various modifications and improvement that case is made, all should fall in the protection domain of claims of the present invention determination.
Claims (7)
1. a kind of preparation method of green plantss specimen is it is characterised in that comprise the following steps:
(1) fresh, healthy, undamaged green plantss branch and leaf are gathered, cleaning and sterilizing obtains germ-free plant branch and leaf；
(2) prepare copper-bath, as fixative, fixative is heated, then the plant leaf of step (1) is put into fixative
In, it is changed into slowly becoming after green after yellowish-brown from green after plant leaf again and take out；
(3) plant specimen, compacting, bookbinding are rinsed, plastic packaging obtains final product.
2. preparation method according to claim 1 it is characterised in that: described plant be Acer palmatum.
3. preparation method according to claim 1 it is characterised in that: described Acer palmatum is yellow eight zhang.
4. preparation method according to claim 1 it is characterised in that: the green branch and leaf of step (1) carry fruit.
5. preparation method according to claim 1 it is characterised in that: step (1) adopt 70% ethanol disinfection and clear water punching
The mode washed obtains germ-free plant branch and leaf.
6. preparation method according to claim 1 it is characterised in that: the compound method of the copper-bath of step (2) is,
The volume ratio of glacial acetic acid, copper sulfate and water is 40:1:100.
7. preparation method according to claim 1 it is characterised in that: in step (2), when fixative is heated to 80-90 DEG C
When, plant leaf is put in fixative.
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| CN201610719558.4A CN106332870A (en) | 2016-08-24 | 2016-08-24 | Acer palmatum thunb variety acer matsumurae green retention method |
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| CN201610719558.4A CN106332870A (en) | 2016-08-24 | 2016-08-24 | Acer palmatum thunb variety acer matsumurae green retention method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107751189A (en) * | 2017-11-04 | 2018-03-06 | 阜阳师范学院 | Green plants leaves ions exchange microwave drying and protect color method |
| CN108029682A (en) * | 2018-01-04 | 2018-05-15 | 云南欣绿茶花股份有限公司 | A kind of processing method of camellia immortality branches and leaves |
| CN109221095A (en) * | 2018-09-26 | 2019-01-18 | 胡玥瑶 | A kind of production method of green plants humid preparation |
| CN112825850A (en) * | 2021-01-04 | 2021-05-25 | 黑龙江省林业科学院伊春分院 | Efficient color-preserving insect-preventing corrosion-preventing method for plant specimen |
| CN114766473A (en) * | 2022-04-11 | 2022-07-22 | 武汉设计工程学院 | Method for manufacturing plant specimen decorative picture with color preservation |
| CN114982745A (en) * | 2022-04-29 | 2022-09-02 | 濮阳市食品药品检验检测中心 | Method for preparing plant-soaked specimen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1058131A (en) * | 1991-02-12 | 1992-01-29 | 张家口市植物标本站 | The colour-keeping treatment method of transparent plant specimens |
| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
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2016
- 2016-08-24 CN CN201610719558.4A patent/CN106332870A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1058131A (en) * | 1991-02-12 | 1992-01-29 | 张家口市植物标本站 | The colour-keeping treatment method of transparent plant specimens |
| CN101218909A (en) * | 2008-01-28 | 2008-07-16 | 河南中医学院 | Original plant humid preparation producing method |
Non-Patent Citations (1)
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| 杨福全: "绿色蜡叶标本", 《师范教育》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107751189A (en) * | 2017-11-04 | 2018-03-06 | 阜阳师范学院 | Green plants leaves ions exchange microwave drying and protect color method |
| CN108029682A (en) * | 2018-01-04 | 2018-05-15 | 云南欣绿茶花股份有限公司 | A kind of processing method of camellia immortality branches and leaves |
| CN109221095A (en) * | 2018-09-26 | 2019-01-18 | 胡玥瑶 | A kind of production method of green plants humid preparation |
| CN112825850A (en) * | 2021-01-04 | 2021-05-25 | 黑龙江省林业科学院伊春分院 | Efficient color-preserving insect-preventing corrosion-preventing method for plant specimen |
| CN114766473A (en) * | 2022-04-11 | 2022-07-22 | 武汉设计工程学院 | Method for manufacturing plant specimen decorative picture with color preservation |
| CN114982745A (en) * | 2022-04-29 | 2022-09-02 | 濮阳市食品药品检验检测中心 | Method for preparing plant-soaked specimen |
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| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 636600 Tissue Culture Workshop Building of Rare and Colorful Leaf Plant Varieties in Guangwu Mountain, Sanshe, Changtan Village, Zhengzheng Town, Nanjiang County, Bazhong City, Sichuan Province Applicant after: Sichuan Qicai Forestry Co., Ltd. Address before: 621000 Huaishu Village Group 6, Dongyu Town, Nanjiang County, Bazhong City, Sichuan Province Applicant before: SICHUAN QICAI FORESTRY INDUSTRY DEVELOPMENT CO., LTD. |
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Application publication date: 20170118 |