CN106324255A - Special protein liquid compound quality control serum with high stability for biochemistry - Google Patents
Special protein liquid compound quality control serum with high stability for biochemistry Download PDFInfo
- Publication number
- CN106324255A CN106324255A CN201610674109.2A CN201610674109A CN106324255A CN 106324255 A CN106324255 A CN 106324255A CN 201610674109 A CN201610674109 A CN 201610674109A CN 106324255 A CN106324255 A CN 106324255A
- Authority
- CN
- China
- Prior art keywords
- control product
- quality
- biochemistry
- stability
- strong
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2496/00—Reference solutions for assays of biological material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to a special protein liquid compound quality control serum with high stability for biochemistry, and belongs to the technical field of clinical in-vitro detection. The quality control serum is a compound liquid quality control serum which is mainly prepared from protective substances such as a buffer solution, a metal chelating agent, a surfactant, a protective agent, an ionic equilibrium agent and a preservative, and the quality control serum can detect 12 substances of antistreptococcic O, C reactive protein, a complement C3, a complement C4, IgM, IgA, IgG, prealbumin, rheumatoid factors, transferrin, total protein and albumin. Serving as the quality control serum of the 12 substances, the quality control serum has the advantages of being convenient to use and high in stability.
Description
Technical field
The biochemistry special proteins liquid that the present invention relates to a kind of stability strong is combined quality-control product, belongs to clinical vitro detection
Technical field.
Background technology
Biochemistry special proteins quality-control product refers to carrying out special proteins detection project in clinical biochemical detection use process
In a kind of quality control material of detecting system being held and controlling；Clinic uses widely, faces at biochemistry
Bed detection in special proteins detection project the most, common are streptococcus O, alpha-fetoprotein, c reactive protein, Complement C_3,
The multiple biochemical projects such as Complement C4, immunoglobulin IGA, immunoglobulin IGG, immunoglobulin IGM, prealbumin；At present
The Quality Control of the special proteins used mostly is man-to-man single project, or the quality-control product of the special proteins being combined for dry powder, and
Seldom there is the quality-control product of the compound special proteins of liquid, and the compound calibration object of liquid is owing to comprising the thing of multiple special proteins
Matter, the process that can carry out early stage directly uses, and saves cost and the operation of configuration, also greatly facilitates clinic simultaneously
Operate with, but owing to different special proteins project performances is different, stable condition is also not quite similar, liquid
Mix under state and be very susceptible to decay or cross reaction, it is impossible to preserve for a long time；Therefore, it is possible to invention one is stable
The special proteins of liquid be combined quality-control product, there is extraordinary Clinical practicability, be also very beneficial for the popularization of clinic and send out
Exhibition.
Therefore according to these problems, the compound of a kind of highly stable liquid comprising 12 kinds of special proteins materials has been invented
Quality-control product.
Summary of the invention
The biochemistry special proteins liquid that the present invention relates to a kind of stability strong is combined quality-control product.
The present invention is obtained through the following steps:
It is as follows that the biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product constituent:
Quality-control product constituent:
Buffer 50mmol/L
Metal-chelator 1 1mmol/L-5mmol/L
Metal-chelator 2 0.1ml/L-0.5 ml/L
Surfactant 1 1ml/L-3ml/L
Surfactant 2 0.5ml/L-1 ml/L
Protective agent 1 30g/L-50g/L
Protective agent 2 7.5mmol/L-10mmol/L
Protective agent 3 1mmol/L-3mmol/L
Ionic equilibrium agent 1 10g/L-20g/L
Ionic equilibrium agent 2 0.5g/L-1g/L
Preservative 0.5g/L-1g/L
Streptococcus O 250KU/L-300kU/K
C reactive protein 50mg/L-55mg/L
Complement C_3 140mg/dL-148mg/dL
Complement C4 30.0mg/dL-35mg/dL
IgM 185mg/dL-190mg/dL
IgA 240mg/dL-250mg/dL
IgG 12g/L-15g/L
Prealbumin 30mg/dL-50mg/dL
Rheumatoid factor 55U/mL-60u/mL
Transferrins 260mg/dL-280mg/dL.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and in described reagent R, buffer is 25 DEG C, pH
It it is " piperazine-1,4-two ethanesulfonic acid buffer of the 50mmol/L of 7.0.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, metal ion chelation agent in described quality-control product
1 is EDTA.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, metal ion chela described in described quality-control product
Mixture 2 is 1-hydroxy ethylidene-1,1-diphosphonic acid.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and described quality-control product surfactant 1 is bent
La Tong-100.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, surfactant described in described quality-control product
2 is alkyl polyglucoside.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and in described quality-control product, protective agent 1 is Sanguis Bovis seu Bubali
Pure albumen.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and in described quality-control product, protective agent 2 is
MgSO4.7H2O。
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and in described quality-control product, protective agent 3 is two sulfur
Threitol.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and described quality-control product intermediate ion poising agent 1 is
Sodium chloride.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and described quality-control product intermediate ion poising agent 2 is
Potassium chloride.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and in described quality-control product, preservative is that methyl is different
Thiazolinone.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, and described reagent is liquid.
Beneficial effects of the present invention:
1) present invention is to comprise the compound liquid special proteins Quality Control of 10 kinds of special proteins and two kinds of routine protein (ALB and TP)
Product；
2) prioritizing selection of the present invention biological buffer PIPES buffer is as buffer system, can not only effectively stablize matter
The pH value of control product, but also can effectively prevent the buffer impact on various special proteins；
3) in invention, the interpolation of two metal ion species chelating agen effectively prevents the heavy metal ion impact on special proteins, to not
Qualitative have bigger help, and the interpolation of surfactant effectively prevents quality-control product muddiness in use, and protectant adds
Add the stability of the most extraordinary raising product.
Accompanying drawing explanation
Accompanying drawing 1 is the stability trendgram of seven kinds of special proteins of CRP, C3, C4, IGM, IGA, PALB, TRF；
Accompanying drawing 2 is the stability trendgram of tri-kinds of albumen of TP, ALB, IGG；
Accompanying drawing 3 is two kinds of special proteins stability trendgrams of ASO, RF.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
It is as follows that the biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product constituent:
Quality-control product constituent:
PIPES 50mmol/L
EDTA 1mmol/L
1-hydroxy ethylidene-1,1-diphosphonic acid 0.1ml/L
TritonX-100 1ml/L
Alkyl polyglucoside 0.5ml/L
Bovine serum albumin 30g/L
7 Magnesium sulfate heptahydrate 7.5mmol/L
Dithiothreitol, DTT 1mmol/L
Sodium chloride 10g/L
Potassium chloride 0.5g/L
Methylisothiazolinone 0.5g/L
Streptococcus O 250KU/L
C reactive protein 50mg/L
Complement C_3 140mg/dL
Complement C4 30.0mg/dL
IgM 185mg/dL
IgA 240mg/dL
IgG 12g/L
Prealbumin 30mg/dL
Rheumatoid factor 55U/mL
Transferrins 260mg/dL.
Embodiment 2
What the present embodiment described is that the biochemistry special proteins liquid that a kind of stability after a kind of material increases is strong is combined Quality Control
Product constituent is as follows:
Quality-control product constituent:
Buffer 50mmol/L
Metal-chelator 1 5mmol/L
Metal-chelator 2 0.5 ml/L
Surfactant 1 3ml/L
Surfactant 21 ml/L
Protective agent 1 50g/L
Protective agent 2 10mmol/L
Protective agent 3 3mmol/L
Ionic equilibrium agent 1 20g/L
Ionic equilibrium agent 2 1g/L
Preservative 1g/L
Streptococcus O 300kU/K
C reactive protein 55mg/L
Complement C_3 148mg/dL
Complement C4 35mg/dL
IgM 190mg/dL
IgA 250mg/dL
IgG 15g/L
Prealbumin 50mg/dL
Rheumatoid factor 60u/mL
Transferrins 80mg/dL.
Embodiment 3
Originally execute that example describes is the verification method of the stability of the quality-control product of the compounded special albumen that the stability of the present invention is strong,
Concrete verification method and step are as follows:
Configuration is completed in embodiment 1 or embodiment 2 the quality-control product of liquid be divided into 13 parts respectively, be stored in 2-8 DEG C
In environment, choose correspondence total protein, albumin, streptococcus O, c reactive protein, Complement C_3, Complement C4, IgA, IgM, IgG,
(12 kinds of reagent are selected from the Shandong Boke limited public affairs of biological industry for prealbumin, rheumatoid factor, 12 kinds of biochemical reagents of transferrins
The test kit that department produces), first automatic clinical chemistry analyzer device is calibrated, and after calibrating successfully, every month detects enforcement respectively
The quality-control product configured in example 1 and embodiment 2, continuous detecting 13 months, observe the situation of change of the result of quality-control product, and shape
Become quality-control product stability tracing figure.
Claims (8)
1. it is as follows that the biochemistry special proteins liquid that a stability is strong is combined quality-control product constituent:
Buffer 50mmol/L
Metal-chelator 1 1mmol/L-5mmol/L
Surfactant 1 1ml/L-3ml/L
Protective agent 1 30g/L-50g/L
Protective agent 3 1mmol/L-3 mmol/L
Ionic equilibrium agent 1 10g/L-20g/L
Streptococcus O 250KU/L-300kU/K
C reactive protein 50mg/L-55mg/L
Complement C_3 140mg/dL-148mg/dL
IgM 185mg/dL-190mg/dL
IgA 240mg/dL-250mg/dL
IgG 12g/L-15g/L
Prealbumin 30mg/dL-50mg/dL
Rheumatoid factor 55U/mL-60u/mL
Transferrins 260mg/dL-280mg/dL.
2. being combined quality-control product according to the biochemistry special proteins liquid that stability a kind of described in claim 1 is strong, its feature exists
In quality-control product, buffer is 25 DEG C, and pH is " piperazine-Isosorbide-5-Nitrae-two ethanesulfonic acid buffer of the 50mmol/L of 7.0.
3. being combined quality-control product according to the biochemistry special proteins liquid that stability a kind of described in claim 1 is strong, its feature exists
In quality-control product, metal ion chelation agent is respectively EDTA and 1-hydroxy ethylidene-1,1-diphosphonic acid, is used in conjunction with.
4. being combined quality-control product according to the biochemistry special proteins liquid that stability a kind of described in claim 1 is strong, its feature exists
It is TritonX-100 and for alkyl polyglucoside in quality-control product surfactant, is used in conjunction with.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, it is characterised in that
In quality-control product, protective agent is respectively bovine serum albumin, MgSO4.7H2O and dithiothreitol, DTT, is used in conjunction with.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, it is characterised in that
Quality-control product intermediate ion poising agent is sodium chloride and potassium chloride, is used in conjunction with.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, it is characterised in that
In quality-control product, preservative is Methylisothiazolinone.
The biochemistry special proteins liquid that a kind of stability is strong is combined quality-control product, it is characterised in that
Quality-control product is liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610674109.2A CN106324255A (en) | 2016-08-17 | 2016-08-17 | Special protein liquid compound quality control serum with high stability for biochemistry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610674109.2A CN106324255A (en) | 2016-08-17 | 2016-08-17 | Special protein liquid compound quality control serum with high stability for biochemistry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106324255A true CN106324255A (en) | 2017-01-11 |
Family
ID=57739537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610674109.2A Pending CN106324255A (en) | 2016-08-17 | 2016-08-17 | Special protein liquid compound quality control serum with high stability for biochemistry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106324255A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107505460A (en) * | 2017-08-07 | 2017-12-22 | 嘉兴博泰生物科技发展有限公司 | C reactive protein quality for POCT controls the preparation method of product |
| CN107703314A (en) * | 2017-11-15 | 2018-02-16 | 迈克生物股份有限公司 | A kind of corticotropin solution and its application |
| CN108107224A (en) * | 2017-12-18 | 2018-06-01 | 郑州安图生物工程股份有限公司 | A kind of ProGRP assays liquid calibration object |
| CN109298176A (en) * | 2018-10-29 | 2019-02-01 | 深圳天深医疗器械有限公司 | Myocarditis quality-control product and preparation method thereof, myocarditis detection kit and myocarditis detection device |
| CN109298173A (en) * | 2018-09-19 | 2019-02-01 | 南京金域医学检验所有限公司 | Immunofluorescence project mixes quality-control product and its preparation method and application |
| CN109406796A (en) * | 2018-12-11 | 2019-03-01 | 迈克生物股份有限公司 | Rheumatoid factor detection reagent box and its detection method |
| CN109613263A (en) * | 2018-12-28 | 2019-04-12 | 山东博科生物产业有限公司 | A kind of blood-lipoids and the compound quality-control product of special proteins |
| CN109781991A (en) * | 2018-12-26 | 2019-05-21 | 深圳开立生物医疗科技股份有限公司 | A kind of c reactive protein saves liquid, calibration object, quality-control product and detection kit |
| CN110780082A (en) * | 2019-11-04 | 2020-02-11 | 上海昆涞实业发展有限公司 | Total complement activity quality control product, reagent, preparation method and application |
| CN110857905A (en) * | 2018-08-22 | 2020-03-03 | 湖南爱威医疗科技有限公司 | Quality control material of excrement analyzer and preparation method thereof |
| CN113234154A (en) * | 2021-04-29 | 2021-08-10 | 四川携光生物技术有限公司 | Rheumatoid factor IgG, IgM and IgA composite quality control product and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1233060A2 (en) * | 2001-02-14 | 2002-08-21 | Sysmex Corporation | Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same |
| CN103604930A (en) * | 2013-11-07 | 2014-02-26 | 北京利德曼生化股份有限公司 | Lipoprotein (a) detection kit |
-
2016
- 2016-08-17 CN CN201610674109.2A patent/CN106324255A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1233060A2 (en) * | 2001-02-14 | 2002-08-21 | Sysmex Corporation | Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same |
| CN103604930A (en) * | 2013-11-07 | 2014-02-26 | 北京利德曼生化股份有限公司 | Lipoprotein (a) detection kit |
Non-Patent Citations (1)
| Title |
|---|
| 林建国等: "多项目特种蛋白复合冻干质控品的研制", 《实验与检验医学》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107505460A (en) * | 2017-08-07 | 2017-12-22 | 嘉兴博泰生物科技发展有限公司 | C reactive protein quality for POCT controls the preparation method of product |
| CN107703314A (en) * | 2017-11-15 | 2018-02-16 | 迈克生物股份有限公司 | A kind of corticotropin solution and its application |
| CN107703314B (en) * | 2017-11-15 | 2018-11-20 | 迈克生物股份有限公司 | A kind of corticotropin solution and its application |
| CN108107224A (en) * | 2017-12-18 | 2018-06-01 | 郑州安图生物工程股份有限公司 | A kind of ProGRP assays liquid calibration object |
| CN110857905A (en) * | 2018-08-22 | 2020-03-03 | 湖南爱威医疗科技有限公司 | Quality control material of excrement analyzer and preparation method thereof |
| CN109298173A (en) * | 2018-09-19 | 2019-02-01 | 南京金域医学检验所有限公司 | Immunofluorescence project mixes quality-control product and its preparation method and application |
| CN109298176A (en) * | 2018-10-29 | 2019-02-01 | 深圳天深医疗器械有限公司 | Myocarditis quality-control product and preparation method thereof, myocarditis detection kit and myocarditis detection device |
| CN109406796A (en) * | 2018-12-11 | 2019-03-01 | 迈克生物股份有限公司 | Rheumatoid factor detection reagent box and its detection method |
| CN109781991A (en) * | 2018-12-26 | 2019-05-21 | 深圳开立生物医疗科技股份有限公司 | A kind of c reactive protein saves liquid, calibration object, quality-control product and detection kit |
| CN109781991B (en) * | 2018-12-26 | 2022-06-24 | 深圳开立生物医疗科技股份有限公司 | C-reactive protein preserving fluid, calibrator, quality control product and detection kit |
| CN109613263A (en) * | 2018-12-28 | 2019-04-12 | 山东博科生物产业有限公司 | A kind of blood-lipoids and the compound quality-control product of special proteins |
| CN110780082A (en) * | 2019-11-04 | 2020-02-11 | 上海昆涞实业发展有限公司 | Total complement activity quality control product, reagent, preparation method and application |
| CN110780082B (en) * | 2019-11-04 | 2023-05-12 | 上海昆涞实业发展有限公司 | Total complement activity quality control product, reagent, preparation method and application |
| CN113234154A (en) * | 2021-04-29 | 2021-08-10 | 四川携光生物技术有限公司 | Rheumatoid factor IgG, IgM and IgA composite quality control product and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106324255A (en) | Special protein liquid compound quality control serum with high stability for biochemistry | |
| US4279885A (en) | Solid phase assay | |
| KR101840525B1 (en) | Vitamin d measurement method and measurement kit | |
| CN108107201A (en) | A kind of cystatin C detection kit and preparation method thereof | |
| CN102246036A (en) | Method for pre-treating sample containing glycosylated hemoglobin | |
| CN109633148B (en) | KL-6 reagent for detecting latex agglutination | |
| JP2014052390A (en) | Method for enhancing sensitivity in immunological measurement, and reagent | |
| JP3600231B1 (en) | Immunoassay | |
| US8431415B2 (en) | Immunoassay using insoluble carrier particles and reagent therefor | |
| JP5137620B2 (en) | Reagent kit for detecting alkaline phosphatase label, reagent kit for immunoassay, and immunoassay method | |
| WO2016133152A2 (en) | Method for suppressing protein adsorption | |
| JP3851807B2 (en) | Method for suppressing prozone phenomenon in immune reaction and reagent for measuring immune reaction | |
| US20140017716A1 (en) | Proteolytic digestion kit with dried reagents | |
| US11046753B2 (en) | Antibody composition and buffer system therefor | |
| JPWO1999050663A6 (en) | Testing methods for IgA nephropathy | |
| Bojesen et al. | Water-phase palmitate concentrations in equilibrium with albumin-bound palmitate in a biological system. | |
| Sakai | Quantitative microdetermination of total SH groups in proteins | |
| Pinkse et al. | A protocol on the use of titanium dioxide chromatography for phosphoproteomics | |
| Jensen et al. | Improved immunoprecipitation to mass spectrometry method for the enrichment of low-abundant protein targets | |
| JP6737284B2 (en) | Stabilizer and stabilizing method | |
| JP2012078161A (en) | Measuring method of measuring target substance in sample, measuring reagent and method for improving measurement value difference | |
| Pass et al. | On the Use of Affinity Chromatography in Demonstrating the Transfer of Thioester‐Bound d‐Phenylalanine from the Light Enzyme of Gramicidin S Synthetase to an Acceptor Site for this Amino Acid on the Heavy Enzyme | |
| Tsai et al. | Mass spectrometry-based proteomics for analysis of hydrophilic phosphopeptides | |
| JP7148948B2 (en) | Protein separation method, protein analysis method, and protein separation kit | |
| JP3034104B2 (en) | Washing solution containing metal ion complexing agent for solid-phase immunoassay and its use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170111 |
|
| WD01 | Invention patent application deemed withdrawn after publication |