CN106319086A - Detection method of bacillus coli in food - Google Patents
Detection method of bacillus coli in food Download PDFInfo
- Publication number
- CN106319086A CN106319086A CN201611025766.0A CN201611025766A CN106319086A CN 106319086 A CN106319086 A CN 106319086A CN 201611025766 A CN201611025766 A CN 201611025766A CN 106319086 A CN106319086 A CN 106319086A
- Authority
- CN
- China
- Prior art keywords
- food
- detection method
- concentration
- concrete steps
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Abstract
The invention discloses a detection method of bacillus coli in food. The method adopts amplification method to take colV specific gene sequence of bacillus coli as target sequence, SYBER Green I as fluorochrome, and then conducts fluorescence quantitative detection and analysis. The concrete process comprises the following steps of, 1, pretreatment; 2, constructing isothermal amplification reaction system; 3, fluorescence quantitative; 4, detection and analysis. The detection method of the invention has high sensitivity, strong specificity and sensibility, lower requirements for template DNA, shorter time consumption and simple operation.
Description
Technical field
The present invention relates to a kind of method of detecting bacterium, colibacillary detection method in specifically a kind of food.
Background technology
Escherichia coli, classify in enterobacteriaceae, belong to Escherichia, and the escherichia coli relevant with human diseases are general
Including five kinds, i.e. enterotoxigenic escherichia coli (ETEC), Escherichia coli (SPEC), Enterohemorrhagic E.coli (EHEC),
Enteroinvasive E．Coli (EIEC) and adhesiveness escherichia coli (EAEC).Escherichia coli are often discharged from people and animal body with feces,
Extensively disseminating in nature, so once detecting escherichia coli, i.e. meaning faecal contamination directly or indirectly, in health
Drinking-water, the excrement source contact scar hygieneic bacteriology index of milk or food etc. it is used on；And owing to escherichia coli are in the external world
Time-to-live is close with some primary bowel pathogen, and its appearance is likely to imply that the existence of some enteric pathogenic bacteria, because of
This this antibacterial is the monitoring of hygiene indicator bacteria generally acknowledged in the world.Prior art, typically uses sterile working, food sample is passed through
Corresponding process after, do certain doubling dilution, cultivate the most under certain condition (as medium component, cultivation temperature and time
Between, pH value, aerobic character etc.), finally at Electronic Speculum, basis of microscopic observation, carry out according to biochemical indicators such as the color of bacterium colony, forms
Differentiate, and calculate the clump count of flat board.The method low cost, particularly requires low to the hardware device of testing laboratory, Zeng Yi
Degree is considered as the classical way of microorganism detection, is also the basic platform of the most various detection method, every country all by
Accreditation, this standard is continuous so far on China edge always, the particularly detection of some pathogenetic bacterias.But, the method is the most loaded down with trivial details, needs
Expend substantial amounts of manpower and materials, and it is low to detect cycle length, sensitivity, it is difficult to meet the most both at home and abroad food safety detection
Requirement.
Summary of the invention
It is an object of the invention to provide colibacillary detection method in a kind of simple in construction, food easy to use,
With the problem solving to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme:
Colibacillary detection method in a kind of food, uses isothermal amplification method, with escherichia coli colV gene specific sequence
For target sequence, SYBER Green I is fluorescent dye, carries out fluorescent quantitation detection subsequently and analyzes；Detailed process includes walking as follows
Rapid:
(1) pretreatment:
In an aseptic environment, being joined by food in LB culture medium, cultivation temperature is 38-42 DEG C, and incubation time is 20-25h, training
Supporting after terminating, Reusability normal saline is carried out, and extracts genomic DNA subsequently；
(2) isothermal amplification system is built:
DNA profiling 2 μ L, SYBER Green I fluorescent dye 0.1 μ L, 10 × Buffer 3.0 μ L, archaeal dna polymerase 15U, ring
Dextrin solution 0.7 μ L, sodium acetate solution 0.5 μ L, dNTP 2 μ L, DNA primer 2 μ L, catalase 2 μ L, hyaluronic acid is molten
Liquid 4 μ L, remaining uses dd H2O complements to 30 μ L；
The OD260/OD280 value of described DNA profiling is 1.7-1.9；
Described SYBER Green I fluorescent dye is 60-65 times and dilutes；
Containing KCl, Tris-HCl(pH 8.3 in described Buffer), sucrose；
Described archaeal dna polymerase uses Pfu polymerase；
The concentration of described cyclodextrin solution is 15-25mmol/L；
The concentration of described sodium acetate solution is 30-40mmol/L；
The OD260/OD280 value of described DNA primer is 1.7-1.9；
The concentration of described hyaluronic acid solution is 20-50mmol/L；
(3) fluorescent quantitation reaction:
Isothermal amplification system is joined in fluorescent quantitation machine, expands subsequently, 95 DEG C of degeneration 5min, 92-95 DEG C of change
Property 1-2min；63 DEG C of annealing 40s；71 DEG C extend 1min；30 circulations, last 72 DEG C extend 10min；
(4) detection is analyzed:
Carrying out quantitative analysis according to quantitative fluorescence analysis software, result is added up.
As the further scheme of the present invention: in concrete steps (1), cultivation temperature is 40 DEG C, incubation time is 23h.
As the further scheme of the present invention: described in concrete steps (2), the OD260/OD280 value of DNA profiling is 1.8.
As the further scheme of the present invention: SYBER Green I fluorescent dye described in concrete steps (2) is 63 times
Dilution.
As the further scheme of the present invention: described in concrete steps (2), the concentration of cyclodextrin solution is 20mmol/L.
As the further scheme of the present invention: described in concrete steps (2), the concentration of sodium acetate solution is 35mmol/L.
As the further scheme of the present invention: described in concrete steps (2), the OD260/OD280 value of DNA primer is 1.8.
As the further scheme of the present invention: described in concrete steps (2), the concentration of hyaluronic acid solution is 35mmol/L.
As the further scheme of the present invention: 93 DEG C of degeneration 1.5min in concrete steps (3).
Compared with prior art, the invention has the beneficial effects as follows:
Using isothermal amplification method, with escherichia coli colV gene specific sequence as target sequence, SYBER Green I is fluorescence dye
Material, carries out fluorescent quantitation detection subsequently and analyzes；Detailed process comprises the steps: (1) pretreatment；(2) isothermal duplication is built anti-
Answer system；(3) fluorescent quantitation reaction；(4) detection is analyzed.The detection method of the present invention is highly sensitive, and specificity and sensitivity are strong,
Requiring relatively low to template DNA, the shortest, method is easy.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the technical scheme of this patent is described in more detail.
Embodiment 1
Colibacillary detection method in a kind of food, uses isothermal amplification method, with escherichia coli colV gene specific sequence
For target sequence, SYBER Green I is fluorescent dye, carries out fluorescent quantitation detection subsequently and analyzes；Detailed process includes walking as follows
Rapid:
(1) pretreatment:
In an aseptic environment, being joined by food in LB culture medium, cultivation temperature is 38 DEG C, and incubation time is 20h, and cultivation terminates
After, Reusability normal saline is carried out, and extracts genomic DNA subsequently；
(2) isothermal amplification system is built:
DNA profiling 2 μ L, SYBER Green I fluorescent dye 0.1 μ L, 10 × Buffer 3.0 μ L, archaeal dna polymerase 15U, ring
Dextrin solution 0.7 μ L, sodium acetate solution 0.5 μ L, dNTP 2 μ L, DNA primer 2 μ L, catalase 2 μ L, hyaluronic acid is molten
Liquid 4 μ L, remaining uses dd H2O complements to 30 μ L；
The OD260/OD280 value of described DNA profiling is 1.7；
Described SYBER Green I fluorescent dye is 60 times of dilutions；
Containing KCl, Tris-HCl(pH 8.3 in described Buffer), sucrose；
Described archaeal dna polymerase uses Pfu polymerase；
The concentration of described cyclodextrin solution is 15mmol/L；
The concentration of described sodium acetate solution is 30mmol/L；
The OD260/OD280 value of described DNA primer is 1.7；
The concentration of described hyaluronic acid solution is 20mmol/L；
(3) fluorescent quantitation reaction:
Isothermal amplification system is joined in fluorescent quantitation machine, expands subsequently, 95 DEG C of degeneration 5min, 92 DEG C of degeneration
1min；63 DEG C of annealing 40s；71 DEG C extend 1min；30 circulations, last 72 DEG C extend 10min；
(4) detection is analyzed:
Carrying out quantitative analysis according to quantitative fluorescence analysis software, result is added up.
Embodiment 2
Colibacillary detection method in a kind of food, uses isothermal amplification method, with escherichia coli colV gene specific sequence
For target sequence, SYBER Green I is fluorescent dye, carries out fluorescent quantitation detection subsequently and analyzes；Detailed process includes walking as follows
Rapid:
(1) pretreatment:
In an aseptic environment, being joined by food in LB culture medium, cultivation temperature is 39 DEG C, and incubation time is 22h, and cultivation terminates
After, Reusability normal saline is carried out, and extracts genomic DNA subsequently；
(2) isothermal amplification system is built:
DNA profiling 2 μ L, SYBER Green I fluorescent dye 0.1 μ L, 10 × Buffer 3.0 μ L, archaeal dna polymerase 15U, ring
Dextrin solution 0.7 μ L, sodium acetate solution 0.5 μ L, dNTP 2 μ L, DNA primer 2 μ L, catalase 2 μ L, hyaluronic acid is molten
Liquid 4 μ L, remaining uses dd H2O complements to 30 μ L；
The OD260/OD280 value of described DNA profiling is 1.7；
Described SYBER Green I fluorescent dye is 61 times of dilutions；
Containing KCl, Tris-HCl(pH 8.3 in described Buffer), sucrose；
Described archaeal dna polymerase uses Pfu polymerase；
The concentration of described cyclodextrin solution is 18mmol/L；
The concentration of described sodium acetate solution is 33mmol/L；
The OD260/OD280 value of described DNA primer is 1.7；
The concentration of described hyaluronic acid solution is 28mmol/L；
(3) fluorescent quantitation reaction:
Isothermal amplification system is joined in fluorescent quantitation machine, expands subsequently, 95 DEG C of degeneration 5min, 92 DEG C of degeneration
1min；63 DEG C of annealing 40s；71 DEG C extend 1min；30 circulations, last 72 DEG C extend 10min；
(4) detection is analyzed:
Carrying out quantitative analysis according to quantitative fluorescence analysis software, result is added up.
Embodiment 3
Colibacillary detection method in a kind of food, uses isothermal amplification method, with escherichia coli colV gene specific sequence
For target sequence, SYBER Green I is fluorescent dye, carries out fluorescent quantitation detection subsequently and analyzes；Detailed process includes walking as follows
Rapid:
(1) pretreatment:
In an aseptic environment, being joined by food in LB culture medium, cultivation temperature is 40 DEG C, and incubation time is 23h, and cultivation terminates
After, Reusability normal saline is carried out, and extracts genomic DNA subsequently；
(2) isothermal amplification system is built:
DNA profiling 2 μ L, SYBER Green I fluorescent dye 0.1 μ L, 10 × Buffer 3.0 μ L, archaeal dna polymerase 15U, ring
Dextrin solution 0.7 μ L, sodium acetate solution 0.5 μ L, dNTP 2 μ L, DNA primer 2 μ L, catalase 2 μ L, hyaluronic acid is molten
Liquid 4 μ L, remaining uses dd H2O complements to 30 μ L；
The OD260/OD280 value of described DNA profiling is 1.8；
Described SYBER Green I fluorescent dye is 63 times of dilutions；
Containing KCl, Tris-HCl(pH 8.3 in described Buffer), sucrose；
Described archaeal dna polymerase uses Pfu polymerase；
The concentration of described cyclodextrin solution is 20mmol/L；
The concentration of described sodium acetate solution is 35mmol/L；
The OD260/OD280 value of described DNA primer is 1.8；
The concentration of described hyaluronic acid solution is 35mmol/L；
(3) fluorescent quantitation reaction:
Isothermal amplification system is joined in fluorescent quantitation machine, expands subsequently, 95 DEG C of degeneration 5min, 93 DEG C of degeneration
1.5min；63 DEG C of annealing 40s；71 DEG C extend 1min；30 circulations, last 72 DEG C extend 10min；
(4) detection is analyzed:
Carrying out quantitative analysis according to quantitative fluorescence analysis software, result is added up.
Embodiment 4
Colibacillary detection method in a kind of food, uses isothermal amplification method, with escherichia coli colV gene specific sequence
For target sequence, SYBER Green I is fluorescent dye, carries out fluorescent quantitation detection subsequently and analyzes；Detailed process includes walking as follows
Rapid:
(1) pretreatment:
In an aseptic environment, being joined by food in LB culture medium, cultivation temperature is 41 DEG C, and incubation time is 24h, and cultivation terminates
After, Reusability normal saline is carried out, and extracts genomic DNA subsequently；
(2) isothermal amplification system is built:
DNA profiling 2 μ L, SYBER Green I fluorescent dye 0.1 μ L, 10 × Buffer 3.0 μ L, archaeal dna polymerase 15U, ring
Dextrin solution 0.7 μ L, sodium acetate solution 0.5 μ L, dNTP 2 μ L, DNA primer 2 μ L, catalase 2 μ L, hyaluronic acid is molten
Liquid 4 μ L, remaining uses dd H2O complements to 30 μ L；
The OD260/OD280 value of described DNA profiling is 1.9；
Described SYBER Green I fluorescent dye is 64 times of dilutions；
Containing KCl, Tris-HCl(pH 8.3 in described Buffer), sucrose；
Described archaeal dna polymerase uses Pfu polymerase；
The concentration of described cyclodextrin solution is 22mmol/L；
The concentration of described sodium acetate solution is 38mmol/L；
The OD260/OD280 value of described DNA primer is 1.9；
The concentration of described hyaluronic acid solution is 45mmol/L；
(3) fluorescent quantitation reaction:
Isothermal amplification system is joined in fluorescent quantitation machine, expands subsequently, 95 DEG C of degeneration 5min, 94 DEG C of degeneration
2min；63 DEG C of annealing 40s；71 DEG C extend 1min；30 circulations, last 72 DEG C extend 10min；
(4) detection is analyzed:
Carrying out quantitative analysis according to quantitative fluorescence analysis software, result is added up.
Embodiment 5
Colibacillary detection method in a kind of food, uses isothermal amplification method, with escherichia coli colV gene specific sequence
For target sequence, SYBER Green I is fluorescent dye, carries out fluorescent quantitation detection subsequently and analyzes；Detailed process includes walking as follows
Rapid:
(1) pretreatment:
In an aseptic environment, being joined by food in LB culture medium, cultivation temperature is 42 DEG C, and incubation time is 25h, and cultivation terminates
After, Reusability normal saline is carried out, and extracts genomic DNA subsequently；
(2) isothermal amplification system is built:
DNA profiling 2 μ L, SYBER Green I fluorescent dye 0.1 μ L, 10 × Buffer 3.0 μ L, archaeal dna polymerase 15U, ring
Dextrin solution 0.7 μ L, sodium acetate solution 0.5 μ L, dNTP 2 μ L, DNA primer 2 μ L, catalase 2 μ L, hyaluronic acid is molten
Liquid 4 μ L, remaining uses dd H2O complements to 30 μ L；
The OD260/OD280 value of described DNA profiling is 1.9；
Described SYBER Green I fluorescent dye is 65 times of dilutions；
Containing KCl, Tris-HCl(pH 8.3 in described Buffer), sucrose；
Described archaeal dna polymerase uses Pfu polymerase；
The concentration of described cyclodextrin solution is 25mmol/L；
The concentration of described sodium acetate solution is 40mmol/L；
The OD260/OD280 value of described DNA primer is 1.9；
The concentration of described hyaluronic acid solution is 50mmol/L；
(3) fluorescent quantitation reaction:
Isothermal amplification system is joined in fluorescent quantitation machine, expands subsequently, 95 DEG C of degeneration 5min, 95 DEG C of degeneration
2min；63 DEG C of annealing 40s；71 DEG C extend 1min；30 circulations, last 72 DEG C extend 10min；
(4) detection is analyzed:
Carrying out quantitative analysis according to quantitative fluorescence analysis software, result is added up.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment party
Formula, in the ken that one skilled in the relevant art is possessed, it is also possible on the premise of without departing from this patent objective
Various changes can be made.
Claims (9)
1. colibacillary detection method in a food, it is characterised in that use isothermal amplification method, with escherichia coli colV
Gene specific sequence is target sequence, and SYBER Green I is fluorescent dye, carries out fluorescent quantitation detection subsequently and analyzes；Concrete mistake
Journey comprises the steps:
(1) pretreatment:
In an aseptic environment, being joined by food in LB culture medium, cultivation temperature is 38-42 DEG C, and incubation time is 20-25h, training
Supporting after terminating, Reusability normal saline is carried out, and extracts genomic DNA subsequently；
(2) isothermal amplification system is built:
DNA profiling 2 μ L, SYBER Green I fluorescent dye 0.1 μ L, 10 × Buffer 3.0 μ L, archaeal dna polymerase 15U, ring
Dextrin solution 0.7 μ L, sodium acetate solution 0.5 μ L, dNTP 2 μ L, DNA primer 2 μ L, catalase 2 μ L, hyaluronic acid is molten
Liquid 4 μ L, remaining uses dd H2O complements to 30 μ L；
The OD260/OD280 value of described DNA profiling is 1.7-1.9；
Described SYBER Green I fluorescent dye is 60-65 times and dilutes；
Containing KCl, Tris-HCl(pH 8.3 in described Buffer), sucrose；
Described archaeal dna polymerase uses Pfu polymerase；
The concentration of described cyclodextrin solution is 15-25mmol/L；
The concentration of described sodium acetate solution is 30-40mmol/L；
The OD260/OD280 value of described DNA primer is 1.7-1.9；
The concentration of described hyaluronic acid solution is 20-50mmol/L；
(3) fluorescent quantitation reaction:
Isothermal amplification system is joined in fluorescent quantitation machine, expands subsequently, 95 DEG C of degeneration 5min, 92-95 DEG C of change
Property 1-2min；63 DEG C of annealing 40s；71 DEG C extend 1min；30 circulations, last 72 DEG C extend 10min；
(4) detection is analyzed:
Carrying out quantitative analysis according to quantitative fluorescence analysis software, result is added up.
Colibacillary detection method in food the most according to claim 1, it is characterised in that training in concrete steps (1)
Foster temperature is 40 DEG C, and incubation time is 23h.
Colibacillary detection method in food the most according to claim 1, it is characterised in that institute in concrete steps (2)
The OD260/OD280 value stating DNA profiling is 1.8.
Colibacillary detection method in food the most according to claim 1, it is characterised in that institute in concrete steps (2)
Stating SYBER Green I fluorescent dye is 63 times of dilutions.
Colibacillary detection method in food the most according to claim 1, it is characterised in that institute in concrete steps (2)
The concentration stating cyclodextrin solution is 20mmol/L.
Colibacillary detection method in food the most according to claim 1, it is characterised in that institute in concrete steps (2)
The concentration stating sodium acetate solution is 35mmol/L.
Colibacillary detection method in food the most according to claim 1, it is characterised in that institute in concrete steps (2)
The OD260/OD280 value stating DNA primer is 1.8.
Colibacillary detection method in food the most according to claim 1, it is characterised in that institute in concrete steps (2)
The concentration stating hyaluronic acid solution is 35mmol/L.
Colibacillary detection method in food the most according to claim 1, it is characterised in that in concrete steps (3) 93
DEG C degeneration 1.5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611025766.0A CN106319086A (en) | 2016-11-22 | 2016-11-22 | Detection method of bacillus coli in food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611025766.0A CN106319086A (en) | 2016-11-22 | 2016-11-22 | Detection method of bacillus coli in food |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106319086A true CN106319086A (en) | 2017-01-11 |
Family
ID=57816346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611025766.0A Pending CN106319086A (en) | 2016-11-22 | 2016-11-22 | Detection method of bacillus coli in food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106319086A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779584A (en) * | 2015-12-18 | 2016-07-20 | 珠海出入境检验检疫局检验检疫技术中心 | Primer and probe group for detecting escherichia coli O111, kit and PCR (polymerase chain reaction) detection method |
-
2016
- 2016-11-22 CN CN201611025766.0A patent/CN106319086A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105779584A (en) * | 2015-12-18 | 2016-07-20 | 珠海出入境检验检疫局检验检疫技术中心 | Primer and probe group for detecting escherichia coli O111, kit and PCR (polymerase chain reaction) detection method |
Non-Patent Citations (2)
| Title |
|---|
| QIUMEI SHI等: "PCR Detection of Virulence Genes Colv，Stxs and HlyE of Escherichia coli，2044-2047", 《AGRICULTURAL SCIENCE & TECHNOLOGY》 * |
| 李伟，黄彬主编: "《分子诊断学》", 30 September 2015, 中国医药科技出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rajapaksha et al. | A review of methods for the detection of pathogenic microorganisms | |
| Lei et al. | Digital PCR for accurate quantification of pathogens: Principles, applications, challenges and future prospects | |
| CN107022644A (en) | Six kinds of multiple LAMP detection primers of food-borne pathogens, detection kit and detection method in fruits and vegetables | |
| CN105524985A (en) | Detection of nucleic acids in crude matrices | |
| CN106191298A (en) | A kind of method detecting vibrio parahaemolyticus Vibrio parahaemolyticus | |
| Tang et al. | Development and clinical verification of a loop-mediated isothermal amplification method for detection of Salmonella species in suspect infected ducks | |
| CN103436602A (en) | Kit and method for simultaneous detection of Staphylococcus aureus gene and Escherichia coli gene by using dual molecular beacon-LAMP process | |
| Zang et al. | Can a visual loop-mediated isothermal amplification assay stand out in different detection methods when monitoring Campylobacter jejuni from diverse sources of samples? | |
| Yamazaki | Sensitive and rapid detection of Campylobacter jejuni and Campylobacter coli using loop-mediated isothermal amplification | |
| CN106591416A (en) | Coloring culture medium for detecting food-borne pathogenic Yersinia | |
| US20220098645A1 (en) | Fast and portable microfluidic detection system as an alternative to salmonella's classical culture method | |
| CN103627812A (en) | Quadruple-PCR (polymerase chain reaction) quick detection kit for main pathogenic bacteria of cow mastitis | |
| Milton et al. | Development of a novel visual detection technique for Campylobacter jejuni in chicken meat and caecum using polymerase spiral reaction (PSR) with pre-added dye | |
| WO2015184454A1 (en) | Clostridium difficile culture medium | |
| CN101875967B (en) | Method for quickly testing food-borne pathogenic bacteria | |
| CN105274193A (en) | Mycobacterium tuberculosis constant temperature rapid detection kit through LAMP method and detection method | |
| CN102936625B (en) | Molecular motor biosensor kit used in vibrio parahaemolyticus molecular typing | |
| CN104673885A (en) | Detection kit and detection method for I-form legionella pneumophila | |
| CN103436623A (en) | Rapid detection kit for viable salmonella in food and use method thereof | |
| CN106319086A (en) | Detection method of bacillus coli in food | |
| CN108220460B (en) | Food-borne streptococcus pyogenes LAMP primer group, kit and application | |
| KR101846182B1 (en) | Primer sets for simultaneous detection of Staphylococcus aureus, Bacillus cereus and Salmonella spp., polymerase chain reaction kit thereof | |
| CN112111584A (en) | Method for rapidly detecting escherichia coli in water | |
| CN106701966B (en) | Rapid detection method for pathogenic microorganisms based on analysis of PCR (polymerase chain reaction) byproduct pyrophosphoric acid | |
| RU2360004C1 (en) | Method of detecting microorganisms of genus salmonella |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |