CN106318856A - Cell enrichment device - Google Patents

Cell enrichment device Download PDF

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Publication number
CN106318856A
CN106318856A CN201610679549.7A CN201610679549A CN106318856A CN 106318856 A CN106318856 A CN 106318856A CN 201610679549 A CN201610679549 A CN 201610679549A CN 106318856 A CN106318856 A CN 106318856A
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CN
China
Prior art keywords
cell
upper cover
installing
slide
fixed block
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610679549.7A
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Chinese (zh)
Inventor
马杰
魏冰
任鹏飞
刘红霞
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Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Individual filed Critical Individual
Priority to CN201610679549.7A priority Critical patent/CN106318856A/en
Publication of CN106318856A publication Critical patent/CN106318856A/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a cell enrichment device which comprises a base and an upper cover buckled on the base in a covering mode. A through hole used for installing a filtrate pipe fixing block is vertically formed in the upper cover; a step trough with two layers is formed in the upper portion of the base, the upper step of the step trough is a filter paper tank used for installing filter paper, and the lower step pf the step trough is a slide tank used for installing a slide; the bottom face of the slide tank is provided with an observation hole of which the position is consistent with the position of a center hole of the filtrate pipe fixing block. According to the cell enrichment device, by means of the capillary action, liquid is slowly dissociated, and cells can rapidly and stably fall onto the slide without changing the original form of the cells.

Description

A kind of cell-enriching device
Technical field
The present invention relates to cell extraction processing equipment field, particularly relate to a kind of cell-enriching device.
Background technology
Existing cell extraction method is to use centrifugation apparatus, is thrown on slide by cell, although this method is quick, but Cellular morphology can be made to change during centrifugal, be unfavorable for that diagnosis and treatment judge.
Summary of the invention
The present invention seeks to provide a kind of cell-enriching device for above-mentioned the deficiencies in the prior art.
The present invention realizes above-mentioned purpose and is adopted the technical scheme that: a kind of cell-enriching device, and it includes base, and The upper cover being fastened on base；The through hole being vertically arranged with for installing filtrate pipe fixed block is covered on described；At the described end Seat offers the step trough of two-layer above, and wherein the upper stage rank of step trough are the filter paper groove for installing filter paper, lower stage rank For the slide groove for installing slide；The central hole location phase one with filtrate pipe fixed block is offered in the bottom surface of described slide groove The observation port caused.
Further, it is provided with in the centre bore of described filtrate pipe fixed block for coordinating fixing spiral type with filtrate pipe Mouth hanging groove.
Further, it is articulated and connected by axle between described upper cover and the rear end of base；Described upper cover sidepiece is provided with use Location screw in fixing filtrate pipe fixed block；The front end of described upper cover is provided with for buckling the card bottom pan frontward end Button.
The principle of the present invention and providing the benefit that: the cell-enriching device of the present invention is to utilize capillarity, by liquid Detaching slowly, enable cell quick and stable falls on slide, does not change the original form of cell；In use, first will Cell sheet glass is placed in the slide groove of bottom, is then placed in filter paper groove by cell filter paper, and covers at Cell sheet glass Top, then snaps together upper cover and base with buckle；Then the filtered capillaries of the blood of cell to be separated will be loaded with It is inserted in the endoporus of fixed block and fixes with location screw, along with the filtrate oozed from filtered capillaries is by cell filter paper Separating, be integrated on Cell sheet glass by hemocyte, period can pass through observation port observation of cell enrichment state on slide, though its So speed is relatively slow, but will not destroy the original form of cell, beneficially later stage by microscope to the observation of cell and analysis.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the present invention.
In figure: 1, upper cover, 2, base, 3, axle, 4, filtrate pipe fixed block, 5, location screw, 6, filter paper groove, 7, slide groove, 8, observation port, 9, buckle.
Detailed description of the invention
The present invention is below with reference to accompanying drawing further description.
As it is shown in figure 1, the cell-enriching device of the present invention includes base 2, and the upper cover 1 being fastened on base；Institute State and cover the through hole being vertically arranged with for installing filtrate pipe fixed block 4；The ladder of two-layer is offered at described submounts Groove, wherein the upper stage rank of step trough are the filter paper groove 6 for installing filter paper, and lower stage rank are the slide groove for installing slide 7；The observation port 8 consistent with the central hole location of filtrate pipe fixed block 4 is offered in the bottom surface of described slide groove；Described filtrate It is provided with in the centre bore of pipe fixed block 4 for coordinating fixing spiral type mouth hanging groove with filtrate pipe.
It is articulated and connected by axle 3 between upper cover of the present invention and the rear end of base；Upper cover sidepiece is provided with for fixing The location screw 5 of filtrate pipe fixed block；The front end of upper cover is provided with for buckling the buckle 9 bottom pan frontward end.

Claims (5)

1. a cell-enriching device, it is characterised in that: it includes base (2), and the upper cover (1) being fastened on base；? The through hole being vertically arranged with for installing filtrate pipe fixed block (4) is covered on described；Two-layer is offered at described submounts Step trough, wherein the upper stage rank of step trough are the filter paper groove (6) for installing filter paper, and lower stage rank are for installing slide Slide groove (7)；The observation port consistent with the central hole location of filtrate pipe fixed block (4) is offered in the bottom surface of described slide groove (8)。
Cell-enriching device the most according to claim 1, it is characterised in that: the centre bore of described filtrate pipe fixed block (4) Inside it is provided with for coordinating fixing spiral type mouth hanging groove with filtrate pipe.
Cell-enriching device the most according to claim 1, it is characterised in that: pass through between described upper cover and the rear end of base Axle (3) is articulated and connected.
Cell-enriching device the most according to claim 1, it is characterised in that: described upper cover sidepiece is provided with for fixing filter The location screw (5) of liquid pipe fixed block.
Cell-enriching device the most according to claim 1, it is characterised in that: the front end of described upper cover is provided with for buckling Buckle (9) bottom pan frontward end.
CN201610679549.7A 2016-08-11 2016-08-11 Cell enrichment device Pending CN106318856A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610679549.7A CN106318856A (en) 2016-08-11 2016-08-11 Cell enrichment device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610679549.7A CN106318856A (en) 2016-08-11 2016-08-11 Cell enrichment device

Publications (1)

Publication Number Publication Date
CN106318856A true CN106318856A (en) 2017-01-11

Family

ID=57742968

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610679549.7A Pending CN106318856A (en) 2016-08-11 2016-08-11 Cell enrichment device

Country Status (1)

Country Link
CN (1) CN106318856A (en)

Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2927191A1 (en) * 1979-07-05 1981-01-15 Rush Presbyterian St Luke Preparation of slide mounted blood sample - including exposure to fixing agent to maintain cell morphology
CN2044981U (en) * 1988-06-03 1989-09-27 么崇正 Cell sheet glass
KR20020041172A (en) * 2000-11-27 2002-06-01 이면우 Automatic Cytology Preparing System
US20080138852A1 (en) * 2004-02-13 2008-06-12 Winkelman James W Identification of blood elements using inverted microscopy
CN201184857Y (en) * 2008-03-25 2009-01-21 肖国林 Clamp for preparing liquid-based thin layer cell
CN201697820U (en) * 2010-04-28 2011-01-05 陈隆玉 Quick batch tabletting device of exfoliated cells
CN201749047U (en) * 2010-07-10 2011-02-16 邱加伟 Rotary device for production of liquid-based thin cell smear
US20120322046A1 (en) * 2010-03-05 2012-12-20 Konica Minolta Holdings, Inc. Method for detecting cell and cell detecting system
CN103103107A (en) * 2013-01-24 2013-05-15 大连医科大学附属第一医院 Convenient body fluid cell precipitation instrument and using method thereof
CN103117102A (en) * 2012-12-18 2013-05-22 浙江建安检测研究院有限公司 Point source manufacturing method and capillary tube used for positron emission tomography (PET) detection
CN203203853U (en) * 2013-01-30 2013-09-18 慈溪市三阳医疗科技有限公司 Liquid based thin-layer cell free settling and flaking device
CN204422302U (en) * 2015-02-10 2015-06-24 刘学亮 Clinical examination smear machine
CN105067397A (en) * 2015-07-17 2015-11-18 邱加伟 Liquid-based thin cell slide preparation device
CN205856466U (en) * 2016-08-11 2017-01-04 马杰 A kind of cell-enriching device

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2927191A1 (en) * 1979-07-05 1981-01-15 Rush Presbyterian St Luke Preparation of slide mounted blood sample - including exposure to fixing agent to maintain cell morphology
CN2044981U (en) * 1988-06-03 1989-09-27 么崇正 Cell sheet glass
KR20020041172A (en) * 2000-11-27 2002-06-01 이면우 Automatic Cytology Preparing System
US20080138852A1 (en) * 2004-02-13 2008-06-12 Winkelman James W Identification of blood elements using inverted microscopy
CN201184857Y (en) * 2008-03-25 2009-01-21 肖国林 Clamp for preparing liquid-based thin layer cell
US20120322046A1 (en) * 2010-03-05 2012-12-20 Konica Minolta Holdings, Inc. Method for detecting cell and cell detecting system
CN201697820U (en) * 2010-04-28 2011-01-05 陈隆玉 Quick batch tabletting device of exfoliated cells
CN201749047U (en) * 2010-07-10 2011-02-16 邱加伟 Rotary device for production of liquid-based thin cell smear
CN103117102A (en) * 2012-12-18 2013-05-22 浙江建安检测研究院有限公司 Point source manufacturing method and capillary tube used for positron emission tomography (PET) detection
CN103103107A (en) * 2013-01-24 2013-05-15 大连医科大学附属第一医院 Convenient body fluid cell precipitation instrument and using method thereof
CN203203853U (en) * 2013-01-30 2013-09-18 慈溪市三阳医疗科技有限公司 Liquid based thin-layer cell free settling and flaking device
CN204422302U (en) * 2015-02-10 2015-06-24 刘学亮 Clinical examination smear machine
CN105067397A (en) * 2015-07-17 2015-11-18 邱加伟 Liquid-based thin cell slide preparation device
CN205856466U (en) * 2016-08-11 2017-01-04 马杰 A kind of cell-enriching device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
魏明竞主编: "《临床检验学》", 科学技术文献出版重庆分社, pages: 268 *

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