CN106317252A - Method for extracting pectin polysaccharides from soybean hulls - Google Patents
Method for extracting pectin polysaccharides from soybean hulls Download PDFInfo
- Publication number
- CN106317252A CN106317252A CN201610698924.2A CN201610698924A CN106317252A CN 106317252 A CN106317252 A CN 106317252A CN 201610698924 A CN201610698924 A CN 201610698924A CN 106317252 A CN106317252 A CN 106317252A
- Authority
- CN
- China
- Prior art keywords
- soybean cover
- soybean
- fermentation
- pectin polysaccharide
- cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a method for extracting pectin polysaccharides from soybean hulls. The method includes (1) homogenizing, (2) fermenting, (3) concentrating, (4) decoloring, (5) drying and (6) packing. The method for extracting the pectin polysaccharides from the soybean hulls has the advantages that the pectin polysaccharides are faint yellow, the method is simple, efficient and capable of achieving large-scale production, working efficiency is improved by 11.7%, yield reaches 32.83%, purity is 76.43% and production cost is saved by 13.4%; since the soybean hulls are ground and homogenized by direct addition of water, a great amount of soaking time and production cost are saved; through fermentation of multiple kinds of lactic acid bacteria, the pectin polysaccharides separate out more easily under the joint action of multiple kinds of fermentation products, so that the yield is increased fundamentally; soybean hull fermentation liquor only needs to be heated and boiled for a short time prior to concentration, and accordingly energy is saved and ethyl alcohol consumption is reduced; low-temperature precipitation after addition of ethyl alcohol promotes the pectin polysaccharides to generate flocculent precipitate, so that precipitating time is shortened remarkably.
Description
Technical field
The invention mainly relates to the extraction field of pectin polysaccharide, particularly relate to the extraction side of pectin polysaccharide in a kind of soybean cover
Method.
Background technology
Soybean cover is the epidermis of soybean seed, accounts for about the 8% of whole Semen sojae atricolor weight.Along with soybean peeling oil-producing technique
Commonly used, create substantial amounts of soybean cover by-product.Soybean cover is directly used in feedstuff, in order to not affect the mouthfeel of feedstuff,
The usage amount of soybean cover and limited, the economic benefit of generation is the most insignificant.Soybean cover is carried out deep processing, extracts pectin
Polysaccharide, twice laid, produce huge added value, hence it is evident that improve the income of plantation peasant household.
The class complexity polysaccharide that pectin is present on plant cell wall, its chemical composition and molecular structure are the completeest
All clear Chu.As generally acknowledged safety food additive, pectin acts not only as thickening agent, stabilizer, gel, additionally it is possible to protect
Protect cardiovascular and cerebrovascular vessel, promote gastrointestinal function, hemostasis, removing toxic substances, treat diabetes, anticancer.At present, the extracting method of pectin polysaccharide includes
Acid hydrolyzation, enzymatic isolation method and ion exchange etc., but all there is the shortcoming that operating procedure is complicated, extraction ratio is low, use toxic reagent,
Extraction cost is high, and can produce a large amount of waste water, pollutes environment.
Summary of the invention
In order to make up the defect of prior art, it is an object of the invention to provide the extraction side of pectin polysaccharide in a kind of soybean cover
Method.
The extracting method of pectin polysaccharide in a kind of soybean cover, specifically includes following steps:
(1) homogenizing: soybean cover pulverized, crosses 80 ~ 100 mesh sieves, obtains Semen sojae atricolor corium farinosum, adds soybean cover grain weight amount in Semen sojae atricolor corium farinosum
The distilled water of 4 ~ 5 times amount, is heated to 71 ~ 73 DEG C, after 36 ~ 38MPa homogenizing 60 ~ 70 seconds, in 13 ~ 15MPa homogenizing 30 ~ 40 seconds,
Save and soak and beating time, using water wisely, prevent nutrition leak, be cooled to 45 ~ 50 DEG C, obtain Semen sojae atricolor latex；
(2) fermentation: adding lactic acid bacteria in Semen sojae atricolor latex, making concentration is 2 ~ 3% (v/v), and mix homogeneously is placed in 40 ~ 42 DEG C of constant temperature
Ferment 15 ~ 17 hours, decomposing soybean Intradermal protein, reduce impurity, slowly regulate pH, promote that pectin polysaccharide separates out, 8000 turns/
Minutes centrifugal 10 ~ 12 minutes, go precipitation, obtain soybean cover fermentation liquid；
(3) concentrate: soybean cover fermentation liquid is heated to 90 ~ 95 DEG C, is incubated 20 ~ 25 minutes, is cooled to 25 ~ 30 DEG C, must concentrate big
Cortex beans fermentation slurry；
(4) decolouring: concentrate the activated carbon of soybean cover fermentation volume of slurry 16 ~ 18% to concentrating addition in soybean cover fermentation slurry, adsorb 80
~ 90 minutes, soybean cover of must decolouring fermentation slurry；
(5) it is dried: the volume fraction adding decolouring soybean cover fermentation volume of slurry 4 ~ 5 times amount in decolouring soybean cover fermentation slurry is 82
The edible alcohol solution of ~ 84%, stirs, and stands 6 ~ 8 hours in 3 ~ 5 DEG C, and 10000 revs/min are centrifuged 8 ~ 10 minutes, and it is heavy to take
Forming sediment, collect supernatant, it is 0 ~ 4% that precipitation is placed in-38 ~-36 DEG C of vacuum lyophilizations to water content, pulverizes, and particle diameter is 28 ~ 30 μm,
Obtain soybean cover pectin polysaccharide；
(6) packaging: vacuum packaging, obtains finished product.
The soybean cover of described step (1), for one or more in Testa Glycines, Semen phaseoli radiati skin, Testa sojae atricolor.
The lactic acid bacteria of described step (2), is made up of the strain of following weight ratio: moral formula lactobacillus: Lactobacillus bulgaricus:
Bacillus acidophilus: bacillus bifidus=24 ~ 26:20 ~ 22:18 ~ 20:18 ~ 20, activates the most respectively, can be used directly.
The heating of described step (3), heats while stirring, and heats up with the speed of 1.6 ~ 1.8 DEG C/min.
The collection supernatant of described step (5), concentrates supernatant, by 40 ~ 42 DEG C of rotary evaporation in vacuo recovered alcohol solution,
Reuse, save great amount of cost.
In described a kind of soybean cover, the extracting method of pectin polysaccharide extracts the pectin polysaccharide obtained.
The invention have the advantage that the extracting method of pectin polysaccharide in a kind of soybean cover that the present invention provides, product is yellowish
Color, method is simply efficient, can carry out large-scale production, and work efficiency improves 11.7%, and yield reaches 32.83%, and purity is
77.34%, save production cost 13.4%；Directly add water homogenisation after soybean cover being pulverized, save a large amount of soak time, save and produce
Cost；Fermenting through various lactobacillus, multiple tunning acts on jointly, promotes that pectin polysaccharide separates out, fundamentally improves yield；
Soybean cover fermentation liquid only need to be carried out Short Time Heating and boil concentration, save the energy, reduce ethanol consumption；Carry out low after adding ethanol
Temperature precipitation, promotes that pectin polysaccharide produces flocculation sediment, hence it is evident that shorten the sedimentation time；Do not use any toxic reagent, do not result in
Residual, health environment-friendly.
Detailed description of the invention
With specific embodiment, the present invention is described below.
Embodiment 1
The extracting method of pectin polysaccharide in a kind of soybean cover, specifically includes following steps:
(1) homogenizing: pulverized by Testa Glycines, crosses 80 mesh sieves, obtains Semen sojae atricolor corium farinosum, adds soybean cover grain weight amount 4 times in Semen sojae atricolor corium farinosum
The distilled water of amount, is heated to 71 DEG C, after 36MPa homogenizing 60 seconds, in 13MPa homogenizing 30 seconds, saves and soaks and beating time, joint
About with water, prevent nutrition leak, be cooled to 45 ~ 50 DEG C, obtain Semen sojae atricolor latex；
(2) fermentation: adding lactic acid bacteria in Semen sojae atricolor latex, making concentration is 2% (v/v), and mix homogeneously is placed in 40 DEG C of ferment at constant temperature
15 hours, decomposing soybean Intradermal protein, reduce impurity, slowly regulate pH, promote that pectin polysaccharide separates out, 8000 revs/min from
The heart 10 minutes, goes precipitation, obtains soybean cover fermentation liquid；
(3) concentrate: soybean cover fermentation liquid is heated to 90 DEG C, is incubated 20 minutes, is cooled to 25 DEG C, soybean cover fermentation must be concentrated
Slurry；
(4) decolouring: concentrate the activated carbon of soybean cover fermentation volume of slurry 16% to concentrating addition in soybean cover fermentation slurry, adsorb 80 points
Clock, soybean cover of must decolouring fermentation slurry；
(5) it is dried: the volume fraction adding decolouring soybean cover fermentation volume of slurry 4 times amount in decolouring soybean cover fermentation slurry is 82%
Edible alcohol solution, stir, stand 6 hours in 3 DEG C, 10000 revs/min centrifugal 8 minutes, takes precipitation, collects supernatant,
It is 0% that precipitation is placed in-38 DEG C of vacuum lyophilizations to water content, pulverizes, and particle diameter is 28 μm, obtains soybean cover pectin polysaccharide；
(6) packaging: vacuum packaging, obtains finished product.
The lactic acid bacteria of described step (2), is made up of the strain of following weight ratio: moral formula lactobacillus: Lactobacillus bulgaricus:
Bacillus acidophilus: bacillus bifidus=24:20:18:18, activates the most respectively, can be used directly.
The heating of described step (3), heats while stirring, and heats up with the speed of 1.6 DEG C/min.
The collection supernatant of described step (5), concentrates supernatant, by 40 DEG C of rotary evaporation in vacuo recovered alcohol solution, enters
Row recycling, saves great amount of cost.
Embodiment 2
The extracting method of pectin polysaccharide in a kind of soybean cover, specifically includes following steps:
(1) homogenizing: by broken for Semen phaseoli radiati corium farinosum, crosses 100 mesh sieves, obtains Semen sojae atricolor corium farinosum, adds soybean cover grain weight amount 5 times in Semen sojae atricolor corium farinosum
The distilled water of amount, is heated to 72 DEG C, after 37MPa homogenizing 65 seconds, in 14MPa homogenizing 35 seconds, saves and soaks and beating time, joint
About with water, prevent nutrition leak, be cooled to 45 ~ 50 DEG C, obtain Semen sojae atricolor latex；
(2) fermentation: adding lactic acid bacteria in Semen sojae atricolor latex, making concentration is 2% (v/v), and mix homogeneously is placed in 41 DEG C of ferment at constant temperature
16 hours, decomposing soybean Intradermal protein, reduce impurity, slowly regulate pH, promote that pectin polysaccharide separates out, 8000 revs/min from
The heart 11 minutes, goes precipitation, obtains soybean cover fermentation liquid；
(3) concentrate: soybean cover fermentation liquid is heated to 93 DEG C, is incubated 23 minutes, is cooled to 28 DEG C, soybean cover fermentation must be concentrated
Slurry；
(4) decolouring: concentrate the activated carbon of soybean cover fermentation volume of slurry 17% to concentrating addition in soybean cover fermentation slurry, adsorb 85 points
Clock, soybean cover of must decolouring fermentation slurry；
(5) it is dried: the volume fraction adding decolouring soybean cover fermentation volume of slurry 5 times amount in decolouring soybean cover fermentation slurry is 83%
Edible alcohol solution, stir, stand 7 hours in 4 DEG C, 10000 revs/min centrifugal 9 minutes, takes precipitation, collects supernatant,
It is 2% that precipitation is placed in-37 DEG C of vacuum lyophilizations to water content, pulverizes, and particle diameter is 29 μm, obtains soybean cover pectin polysaccharide；
(6) packaging: vacuum packaging, obtains finished product.
The lactic acid bacteria of described step (2), is made up of the strain of following weight ratio: moral formula lactobacillus: Lactobacillus bulgaricus:
Bacillus acidophilus: bacillus bifidus=25:21:19:19, activates the most respectively, can be used directly.
The heating of described step (3), heats while stirring, and heats up with the speed of 1.7 DEG C/min.
The collection supernatant of described step (5), concentrates supernatant, by 41 DEG C of rotary evaporation in vacuo recovered alcohol solution, enters
Row recycling, saves great amount of cost.
Embodiment 3
The extracting method of pectin polysaccharide in a kind of soybean cover, specifically includes following steps:
(1) homogenizing: pulverized by Testa sojae atricolor, crosses 100 mesh sieves, obtains Semen sojae atricolor corium farinosum, adds soybean cover grain weight amount 5 times in Semen sojae atricolor corium farinosum
The distilled water of amount, is heated to 73 DEG C, after 38MPa homogenizing 70 seconds, in 15MPa homogenizing 40 seconds, saves and soaks and beating time, joint
About with water, prevent nutrition leak, be cooled to 45 ~ 50 DEG C, obtain Semen sojae atricolor latex；
(2) fermentation: adding lactic acid bacteria in Semen sojae atricolor latex, making concentration is 3% (v/v), and mix homogeneously is placed in 42 DEG C of ferment at constant temperature
17 hours, decomposing soybean Intradermal protein, reduce impurity, slowly regulate pH, promote that pectin polysaccharide separates out, 8000 revs/min from
The heart 12 minutes, goes precipitation, obtains soybean cover fermentation liquid；
(3) concentrate: soybean cover fermentation liquid is heated to 95 DEG C, is incubated 25 minutes, is cooled to 30 DEG C, soybean cover fermentation must be concentrated
Slurry；
(4) decolouring: concentrate the activated carbon of soybean cover fermentation volume of slurry 18% to concentrating addition in soybean cover fermentation slurry, adsorb 90 points
Clock, soybean cover of must decolouring fermentation slurry；
(5) it is dried: the volume fraction adding decolouring soybean cover fermentation volume of slurry 5 times amount in decolouring soybean cover fermentation slurry is 84%
Edible alcohol solution, stir, stand 8 hours in 5 DEG C, 10000 revs/min centrifugal 10 minutes, takes precipitation, in collection
Clearly, it is 4% that precipitation is placed in-36 DEG C of vacuum lyophilizations to water content, pulverizes, and particle diameter is 30 μm, obtains soybean cover pectin polysaccharide；
(6) packaging: vacuum packaging, obtains finished product.
The lactic acid bacteria of described step (2), is made up of the strain of following weight ratio: moral formula lactobacillus: Lactobacillus bulgaricus:
Bacillus acidophilus: bacillus bifidus=26:22:20:20, activates the most respectively, can be used directly.
The heating of described step (3), heats while stirring, and heats up with the speed of 1.8 DEG C/min.
The collection supernatant of described step (5), concentrates supernatant, by 42 DEG C of rotary evaporation in vacuo recovered alcohol solution, enters
Row recycling, saves great amount of cost.
Comparative example
Existing pectin polysaccharide extracting method.
Embodiment and the index evaluation of comparative example pectin polysaccharide extracting method:
Extract the pectin polysaccharide in same breed soybean cover by the method for embodiment and comparative example respectively, and detect, implement
The index evaluation of example and comparative example pectin polysaccharide extracting method is shown in Table 1.
Table 1: embodiment and the index evaluation of comparative example pectin polysaccharide extracting method
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
| Yield/(%) | 32.83 | 32.76 | 32.65 | 13.63 |
| Purity/(%) | 76.43 | 76.86 | 77.34 | 54.42 |
| Improve work efficiency/(%) | 11.7 | 11.3 | 10.9 | — |
| Save production cost/(%) | 13.4 | 13.2 | 12.8 | — |
Note: " " indicate without.
The result of table 1 shows, the extracting method of pectin polysaccharide in the soybean cover of embodiment, and yield is up to 32.83%, purity
77.34%, improve work efficiency 11.7%, save production cost 13.4%, pectin polysaccharide in the soybean cover that the present invention provides is described
Extracting method can be obviously improved existing pectin polysaccharide extracting method.
Claims (6)
1. the extracting method of pectin polysaccharide in a soybean cover, it is characterised in that specifically include following steps:
(1) homogenizing: soybean cover pulverized, crosses 80 ~ 100 mesh sieves, obtains Semen sojae atricolor corium farinosum, adds soybean cover grain weight amount in Semen sojae atricolor corium farinosum
The distilled water of 4 ~ 5 times amount, is heated to 71 ~ 73 DEG C, after 36 ~ 38MPa homogenizing 60 ~ 70 seconds, in 13 ~ 15MPa homogenizing 30 ~ 40 seconds,
It is cooled to 45 ~ 50 DEG C, obtains Semen sojae atricolor latex；
(2) fermentation: adding lactic acid bacteria in Semen sojae atricolor latex, making concentration is 2 ~ 3% (v/v), and mix homogeneously is placed in 40 ~ 42 DEG C of constant temperature
Fermenting 15 ~ 17 hours, 8000 revs/min are centrifuged 10 ~ 12 minutes, go precipitation, obtain soybean cover fermentation liquid；
(3) concentrate: soybean cover fermentation liquid is heated to 90 ~ 95 DEG C, is incubated 20 ~ 25 minutes, is cooled to 25 ~ 30 DEG C, must concentrate big
Cortex beans fermentation slurry；
(4) decolouring: concentrate the activated carbon of soybean cover fermentation volume of slurry 16 ~ 18% to concentrating addition in soybean cover fermentation slurry, adsorb 80
~ 90 minutes, soybean cover of must decolouring fermentation slurry；
(5) it is dried: the volume fraction adding decolouring soybean cover fermentation volume of slurry 4 ~ 5 times amount in decolouring soybean cover fermentation slurry is 82
The edible alcohol solution of ~ 84%, stirs, and stands 6 ~ 8 hours in 3 ~ 5 DEG C, and 10000 revs/min are centrifuged 8 ~ 10 minutes, and it is heavy to take
Forming sediment, collect supernatant, it is 0 ~ 4% that precipitation is placed in-38 ~-36 DEG C of vacuum lyophilizations to water content, pulverizes, and particle diameter is 28 ~ 30 μm,
Obtain soybean cover pectin polysaccharide；
(6) packaging: vacuum packaging, obtains finished product.
The extracting method of pectin polysaccharide in soybean cover the most according to claim 1, it is characterised in that described step (1) big
Cortex beans, for one or more in Testa Glycines, Semen phaseoli radiati skin, Testa sojae atricolor.
The extracting method of pectin polysaccharide in soybean cover the most according to claim 1, it is characterised in that the breast of described step (2)
Acid bacterium, is made up of the strain of following weight ratio: moral formula lactobacillus: Lactobacillus bulgaricus: bacillus acidophilus: bacillus bifidus=24 ~
26:20 ~ 22:18 ~ 20:18 ~ 20, activate the most respectively, can be used directly.
The extracting method of pectin polysaccharide in soybean cover the most according to claim 1, it is characterised in that adding of described step (3)
Heat, heats while stirring, and heats up with the speed of 1.6 ~ 1.8 DEG C/min.
The extracting method of pectin polysaccharide in soybean cover the most according to claim 1, it is characterised in that the receipts of described step (5)
Collection supernatant, concentrates supernatant, by 40 ~ 42 DEG C of rotary evaporation in vacuo recovered alcohol solution.
6. extract, according to the extracting method of pectin polysaccharide in soybean cover described in any one of claim 1 ~ 5, the pectin polysaccharide obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610698924.2A CN106317252A (en) | 2016-08-22 | 2016-08-22 | Method for extracting pectin polysaccharides from soybean hulls |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610698924.2A CN106317252A (en) | 2016-08-22 | 2016-08-22 | Method for extracting pectin polysaccharides from soybean hulls |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106317252A true CN106317252A (en) | 2017-01-11 |
Family
ID=57741666
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610698924.2A Pending CN106317252A (en) | 2016-08-22 | 2016-08-22 | Method for extracting pectin polysaccharides from soybean hulls |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106317252A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114868800A (en) * | 2022-04-07 | 2022-08-09 | 渤海大学 | Hemp seed oil fermented milk and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1826949A (en) * | 2005-10-25 | 2006-09-06 | 山东万得福实业集团有限公司 | Novel method for preparing pectin by soybean seed coat |
| CN102558377A (en) * | 2011-12-23 | 2012-07-11 | 南昌大学 | Preparation method of soybean polysaccharide gum |
| CN103224573A (en) * | 2013-04-24 | 2013-07-31 | 上海交通大学 | Method for extracting pectin from skin of beancurd |
| CN103265647A (en) * | 2013-05-14 | 2013-08-28 | 安徽金枫果胶有限公司 | Method for extracting pectin from waste potato residues |
-
2016
- 2016-08-22 CN CN201610698924.2A patent/CN106317252A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1826949A (en) * | 2005-10-25 | 2006-09-06 | 山东万得福实业集团有限公司 | Novel method for preparing pectin by soybean seed coat |
| CN102558377A (en) * | 2011-12-23 | 2012-07-11 | 南昌大学 | Preparation method of soybean polysaccharide gum |
| CN103224573A (en) * | 2013-04-24 | 2013-07-31 | 上海交通大学 | Method for extracting pectin from skin of beancurd |
| CN103265647A (en) * | 2013-05-14 | 2013-08-28 | 安徽金枫果胶有限公司 | Method for extracting pectin from waste potato residues |
Non-Patent Citations (3)
| Title |
|---|
| 于新等编著: "《大豆加工副产品的综合利用》", 30 June 2013, 中国纺织出版社 * |
| 刘新社等主编: "《果蔬贮藏与加工技术》", 30 April 2014, 中国轻工业出版社 * |
| 申晓琳等主编: "《乳品加工技术》", 31 August 2015, 中国轻工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114868800A (en) * | 2022-04-07 | 2022-08-09 | 渤海大学 | Hemp seed oil fermented milk and preparation method thereof |
| CN114868800B (en) * | 2022-04-07 | 2023-10-20 | 渤海大学 | Fructus cannabis oil fermented milk and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104140474B (en) | A kind of method of utility in comprehensive utilizing shrimp Eriocheir sinensis skin | |
| CN103843970B (en) | Production method for preparing ossein oligopeptide meal, bone oil and bone meal | |
| CN106387924B (en) | Method for extracting suaeda salsa dietary fiber | |
| CN104187746B (en) | The technique that fermentation method prepares Testa Phaseoli radiati water soluble dietary fiber | |
| CN107047921A (en) | A kind of method for preparing albumen powder and polypeptide powder using various vinasse | |
| CN103749959A (en) | Biological fermented bran and processing method thereof | |
| CN105166942A (en) | Method for enzyme-method-assisted microwave-ultrasonic wave synergistic extraction of soluble dietary fiber from soy sauce residues | |
| CN107048361B (en) | Red date and herba cistanches pomegranate rind composite enzyme and preparation method thereof | |
| CN102787013B (en) | Method for fermenting and extracting corn oil by microorganisms | |
| CN106367201A (en) | Fermentation and extraction method for pumpkin seed oil | |
| CN109576324A (en) | A kind of astragalus polyose and its biological extraction method | |
| CN107509915B (en) | Method for reducing cyanide content of cassava residue | |
| CN103388016A (en) | Extraction method of Chinese yam small molecule peptides | |
| CN104789491B (en) | Lichem bacillus strain and its application | |
| CN106317252A (en) | Method for extracting pectin polysaccharides from soybean hulls | |
| CN108148882A (en) | A kind of extracting method of crocodile first peptide | |
| CN105876076A (en) | Method for preparing bone polypeptides through microwave-assisted enzymolysis | |
| CN107446692A (en) | Utilize the integrated approach of melon seeds edible health oil in August | |
| CN103518943A (en) | Method for extracting proteins from rape seed cakes by utilizing multi-frequency ultrasonic waves | |
| CN105111003A (en) | Liquid organic fertilizer and preparing method thereof | |
| CN108029870A (en) | The preparation method of dairy buffalo feed | |
| CN106317240A (en) | Efficient water-soluble soybean polysaccharide extraction method | |
| CN107821803A (en) | The preparation method of brewex's grains dairy buffalo feed | |
| CN106434516A (en) | Preparation method and application for edible fungus selenium-enriched polysaccharide | |
| CN108395468B (en) | Method for recovering protein in bean curd yellow slurry by using astringent persimmon tannin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |