CN106317215A - Method for extracting silk amino acids - Google Patents
Method for extracting silk amino acids Download PDFInfo
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- CN106317215A CN106317215A CN201610713251.3A CN201610713251A CN106317215A CN 106317215 A CN106317215 A CN 106317215A CN 201610713251 A CN201610713251 A CN 201610713251A CN 106317215 A CN106317215 A CN 106317215A
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- silkworm silk
- solubility
- silkworm
- silk
- aminoacid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
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- Gastroenterology & Hepatology (AREA)
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- Tropical Medicine & Parasitology (AREA)
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- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Peptides Or Proteins (AREA)
Abstract
The invention discloses a method for extracting silk amino acids, comprising the srteps of opening cocoons, selecting cocoons, washing cocoons, first degumming, second degumming, cooling, freezing, thawing, drying, grinding, sterilization, and vacuum packaging; the method does not add any chemical materials to soluble silk amino acids extraction; extracted silk amino acids, with high purity and long save period, can be dissolved in water rapidly and produced no smell. It will turn into jelly-like material after boiling water-silk amino acids solution. The method has great convenience application of soluble silk amino acids, and highlights the value of soluble silk amino acids in cosmetic, medical and food.
Description
Technical field
The present invention relates to silkworm silk and utilize technical field, particularly a kind of extraction amino acid whose processing technology of silkworm silk.
Background technology
There is one layer of jelly on the top layer of silkworm silk, and when silkworm cocoons, sericin plays adhesive effect, makes two monofilament be coated on one
Body constitutes silk.This jelly is referred to as sericin, is mainly made up of solubility silkworm silk aminoacid；Account for silkworm silk gross weight
15%-25%.
Sericin is made up of aminoacid such as serine, the aspartic acid etc. of a large amount of side chain band hydrophilic groups, have skin protection,
The functions such as beauty treatment and health care, therefore, have bigger market demand.
In correlation technique, after mostly the common amino acid whose method of silkworm silk of producing is to treat that matured silkworm cocoons, by containing Pupa bombycis
Bombyx bombycis together extracts, and the silkworm body amino acid content that the method is extracted is high, but cannot extract highly purified solubility silkworm silk amino
Acid；The most also, after having unit to improve, Pupa bombycis is taken out, then extracts by chemical technology, although the amount of extraction is big very
Many, extraction cost also relative moderate, but be used for refuting from the amino acid whose chemical substance of silkworm silk, but the natural silkworm silk ammonia of secondary pollution
Base acid.
This makes the silkworm silk aminoacid of China be unable to reach purity and effect of international equal physical product always.
Summary of the invention
In order to overcome the disadvantages mentioned above of prior art, it is an object of the invention to provide the highly purified silkworm silk amino of a kind of extraction
The processing technology of acid.
The technical solution adopted for the present invention to solve the technical problems is: a kind of extraction amino acid whose processing technology of silkworm silk,
Wherein: described manufacturing process steps is as follows:
1) opening cocoon: by Bombyx bombycis opening, opening is not more than 1/3rd of Bombyx bombycis, but must not cut off, and is taken out by Pupa bombycis；
2) selecting silkworm: after removing Pupa bombycis, select inwall without the excremental Bombyx bombycis of silkworm, inwall speckles with the excremental Bombyx bombycis of silkworm and does substandard products,
For making the silk flosssilk wadding of other purposes；
3) wash cocoon: cleaned by the Bombyx bombycis of selection with clear water, remove dust and washable pollutant, then get rid of with drier
Dry, load standby in mesh bag one by one；
4) separate silkworm silk aminoacid: being loaded into the cocoon that mesh bag is standby, high temperature distillation still put into by whole bag, temperature be set to 100 DEG C ~
140 DEG C, cocoon is that 1:1 ~ 3 mix with the ratio of water, after steaming and decocting 1 ~ 4 hour, is poured into by the solubility silkworm silk Freamine Ⅲ obtained
Fluid reservoir cools down；
5) collect remaining silkworm silk aminoacid: add the pure water being not less than 60 DEG C, cocoon is washed 5 ~ 0 minutes, cocoon will press from both sides the silkworm deposited
After Silk Amino Acids washes clean into, the solubility silkworm silk Freamine Ⅲ obtained is poured fluid reservoir cooling；
6) cooling: by step 4) ~ 5) the solubility silkworm silk Freamine Ⅲ in fluid reservoir of gained naturally cools to 40 DEG C；
7) freezing: solubility silkworm silk Freamine Ⅲ to be put into freezing 48 hours of the refrigerating chamber of subzero 2 DEG C, treats solubility silkworm silk ammonia
Base acid solution forms ice cube completely；
8) thaw: solubility silkworm silk Freamine Ⅲ ice cube is put into constant temperature barn, and arranging temperature is 25 DEG C, water-soluble in ice cube
Solving, it is cotton-shaped that solubility silkworm silk aminoacid separates out into mud；
9) filter pressure: under 4 ~ 25 DEG C of dustfree environments, Jiang Shui and the cotton-shaped solubility silkworm silk aminoacid of mud lead to filtration pressure equipment and carry out
Separate；Obtain pureed solubility silkworm silk aminoacid；
10) dry: pureed solubility silkworm silk aminoacid is spread out, the baking machine of 60 DEG C is baked to be completely dried；
11) grind: put dry block solubility silkworm silk aminoacid into grinder, be ground to 200 mesh ~ 5000 mesh, obtain silkworm
Silk Amino Acids；
12) steam kettle sterilizing, it is vacuum-packed.
As a further improvement on the present invention: described fluid reservoir uses metal material to make.
As a further improvement on the present invention: described grinder is nano-level grinder or refiner.
In as a further improvement on the present invention: above-mentioned steps 5), pure water is to obtain after employing 2 ~ 4 grades filter pressure system filters
Pure water.
Compared with prior art, the invention has the beneficial effects as follows:
1) solubility silkworm silk aminoacid is used pure water technique, be stripped out from Bombyx bombycis, it is ensured that the amino acid whose purity of silkworm silk
With natural characteristic.
2) compare the silk sericin that other techniques extract, have in the more stable preservation state of nature, be difficult to
Become damp and rotten, without any abnormal flavour.
3) compare the sericin that other techniques extract, with under the ratio of pure water 1:100, this technique extract silkworm silk
Aminoacid can form normal wet lower stable g., jelly-like gel, makes in skin Caring or edible, the sericin of other techniques then without
This characteristic.
4) free from foreign meter, be more convenient for fine bionic interpolation and use.
5) more variable skin absorption, can be used for the skin amino acid nutrient agent of all cosmetics.
Detailed description of the invention
In conjunction with embodiment, the present invention is further described:
A kind of amino acid whose processing technology of silkworm silk of extracting, wherein: described manufacturing process steps is as follows:
1) opening cocoon: by Bombyx bombycis opening, opening is not more than 1/3rd of Bombyx bombycis, but must not cut off, and is taken out by Pupa bombycis；
2) selecting silkworm: after removing Pupa bombycis, select inwall without the excremental Bombyx bombycis of silkworm, inwall speckles with the excremental Bombyx bombycis of silkworm and does substandard products,
For making the silk flosssilk wadding of other purposes；
3) wash cocoon: cleaned by the Bombyx bombycis of selection with clear water, remove dust and washable pollutant, then get rid of with drier
Dry, load standby in mesh bag one by one；
4) silkworm silk aminoacid is separated: being loaded into the cocoon that mesh bag is standby, high temperature distillation still put into by whole bag, and temperature is set to 120 DEG C, cocoon
It is that 1:2 mixes with the ratio of water, after steaming and decocting 3 hours into, the solubility silkworm silk Freamine Ⅲ obtained is poured fluid reservoir cooling；
5) collect remaining silkworm silk aminoacid: add the pure water being not less than 60 DEG C, cocoon is washed 30 minutes, cocoon will press from both sides the silkworm silk deposited
After aminoacid washes clean into, the solubility silkworm silk Freamine Ⅲ obtained is poured fluid reservoir cooling；
6) cooling: by step 4) ~ 5) the solubility silkworm silk Freamine Ⅲ in fluid reservoir of gained naturally cools to 40 DEG C；
7) freezing: solubility silkworm silk Freamine Ⅲ to be put into freezing 48 hours of the refrigerating chamber of subzero 2 DEG C, treats solubility silkworm silk ammonia
Base acid solution forms ice cube completely；
8) thaw: solubility silkworm silk Freamine Ⅲ ice cube is put into constant temperature barn, and arranging temperature is 25 DEG C, water-soluble in ice cube
Solving, it is cotton-shaped that solubility silkworm silk aminoacid separates out into mud；
9) filter pressure: under 15 DEG C of dustfree environments, Jiang Shui and the cotton-shaped solubility silkworm silk aminoacid of mud lead to filtration pressure equipment to be carried out point
From；Obtain pureed solubility silkworm silk aminoacid；
10) dry: pureed solubility silkworm silk aminoacid is spread out, the baking machine of 60 DEG C is baked to be completely dried；
11) grind: put dry block solubility silkworm silk aminoacid into grinder, be ground to 200 mesh mesh, obtain silkworm silk amino
Acidic group material；Requirement is used according to product, can the most further fine gtinding.
12) steam kettle sterilizing, it is vacuum-packed.
In sum, after those of ordinary skill in the art reads file of the present invention, according to technical scheme and
Technology design makes other various corresponding conversion scheme without creative mental work, belongs to the model that the present invention is protected
Enclose.
Claims (4)
1. one kind is extracted the amino acid whose processing technology of silkworm silk, it is characterised in that: described manufacturing process steps is as follows:
1) opening cocoon: by Bombyx bombycis opening, opening is not more than 1/3rd of Bombyx bombycis, but must not cut off, and is taken out by Pupa bombycis；
2) selecting silkworm: after removing Pupa bombycis, select inwall without the excremental Bombyx bombycis of silkworm, inwall speckles with the excremental Bombyx bombycis of silkworm and does substandard products,
For making the silk flosssilk wadding of other purposes；
3) wash cocoon: cleaned by the Bombyx bombycis of selection with clear water, remove dust and washable pollutant, then get rid of with drier
Dry, load standby in mesh bag one by one；
4) separate silkworm silk aminoacid: being loaded into the cocoon that mesh bag is standby, high temperature distillation still put into by whole bag, temperature be set to 100 DEG C ~
140 DEG C, cocoon is that 1:1 ~ 3 mix with the ratio of water, after steaming and decocting 1 ~ 4 hour, is poured into by the solubility silkworm silk Freamine Ⅲ obtained
Fluid reservoir cools down；
5) collect remaining silkworm silk aminoacid: add the pure water being not less than 60 DEG C, cocoon is washed 5 ~ 0 minutes, cocoon will press from both sides the silkworm deposited
After Silk Amino Acids washes clean into, the solubility silkworm silk Freamine Ⅲ obtained is poured fluid reservoir cooling；
6) cooling: by step 4) ~ 5) the solubility silkworm silk Freamine Ⅲ in fluid reservoir of gained naturally cools to 40 DEG C；
7) freezing: solubility silkworm silk Freamine Ⅲ to be put into freezing 48 hours of the refrigerating chamber of subzero 2 DEG C, treats solubility silkworm silk ammonia
Base acid solution forms ice cube completely；
8) thaw: solubility silkworm silk Freamine Ⅲ ice cube is put into constant temperature barn, and arranging temperature is 25 DEG C, water-soluble in ice cube
Solving, it is cotton-shaped that solubility silkworm silk aminoacid separates out into mud；
9) filter pressure: under 4 ~ 25 DEG C of dustfree environments, Jiang Shui and the cotton-shaped solubility silkworm silk aminoacid of mud lead to filtration pressure equipment and carry out
Separate；Obtain pureed solubility silkworm silk aminoacid；
10) dry: pureed solubility silkworm silk aminoacid is spread out, the baking machine of 60 DEG C is baked to be completely dried；
11) grind: put dry block solubility silkworm silk aminoacid into grinder, be ground to 200 mesh ~ 5000 mesh, obtain silkworm
Silk Amino Acids；
12) steam kettle sterilizing, it is vacuum-packed.
A kind of extraction amino acid whose processing technology of silkworm silk the most according to claim 1, it is characterised in that: described liquid storage
Tank uses metal material to make.
A kind of extraction amino acid whose processing technology of silkworm silk the most according to claim 1, it is characterised in that: described grinder
For nano-level grinder or refiner.
A kind of extraction amino acid whose processing technology of silkworm silk the most according to claim 1, it is characterised in that: above-mentioned steps 5)
Middle pure water is the pure water obtained after employing 2 ~ 4 grades filter pressure system filters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610713251.3A CN106317215A (en) | 2016-08-24 | 2016-08-24 | Method for extracting silk amino acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610713251.3A CN106317215A (en) | 2016-08-24 | 2016-08-24 | Method for extracting silk amino acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106317215A true CN106317215A (en) | 2017-01-11 |
Family
ID=57742578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610713251.3A Pending CN106317215A (en) | 2016-08-24 | 2016-08-24 | Method for extracting silk amino acids |
Country Status (1)
| Country | Link |
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| CN (1) | CN106317215A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110012962A (en) * | 2019-04-23 | 2019-07-16 | 中科国思生物科技研究（广州）有限公司 | A kind of silkworm nest dry plate prepared using silk |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1343733A (en) * | 2000-09-18 | 2002-04-10 | 刘云聪 | Process for recovering sericin of cocoon silk |
| CN105168015A (en) * | 2015-10-13 | 2015-12-23 | 广东中科国丝生物科技有限公司 | Preparation method for protein complex of sarcosine |
-
2016
- 2016-08-24 CN CN201610713251.3A patent/CN106317215A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1343733A (en) * | 2000-09-18 | 2002-04-10 | 刘云聪 | Process for recovering sericin of cocoon silk |
| CN105168015A (en) * | 2015-10-13 | 2015-12-23 | 广东中科国丝生物科技有限公司 | Preparation method for protein complex of sarcosine |
Non-Patent Citations (4)
| Title |
|---|
| 代君君等: "丝胶蛋白粉的制备及其物理性质初探", 《畜牧兽医科学》 * |
| 张雨青: "蚕丝脱胶方法的比较分析", 《蚕业科学》 * |
| 董雪等: "丝胶蛋白的研究与应用综述", 《丝绸》 * |
| 马骏等: "蚕丝丝胶蛋白提取方法的研究", 《安徽农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110012962A (en) * | 2019-04-23 | 2019-07-16 | 中科国思生物科技研究（广州）有限公司 | A kind of silkworm nest dry plate prepared using silk |
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Effective date of registration: 20181017 Address after: 510000 Guangdong Province Nansha District Fengze East Road 106 (self-numbered No. 1 Building) X1301-I2915 (office use only) (JM) Applicant after: Chinese science biology science and technology research (Guangzhou) Co., Ltd. Address before: 510000 X1301-A301, 106 East Feng Ze Road, Nansha District, Guangzhou, Guangdong. Applicant before: GUANGDONG ZHONGKE GUOSI BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD. |
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Application publication date: 20170111 |