CN106317149A - Cordyceps cicadae adenosine preparation and extracting process - Google Patents
Cordyceps cicadae adenosine preparation and extracting process Download PDFInfo
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- CN106317149A CN106317149A CN201610687874.8A CN201610687874A CN106317149A CN 106317149 A CN106317149 A CN 106317149A CN 201610687874 A CN201610687874 A CN 201610687874A CN 106317149 A CN106317149 A CN 106317149A
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- cordyceps cicadae
- adenosine
- cicadae shing
- extraction process
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/167—Purine radicals with ribosyl as the saccharide radical
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
- A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
- A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- Animal Behavior & Ethology (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the field of edible mushrooms. A cordyceps cicadae adenosine preparation comprises the following materials: 10%-30% of sporocarp of cordyceps cicadae, 70%-90% of stroma of cordyceps cicadae and 5% of auxiliary materials, and the sum of the three components is 100%. The invention further relates to a cordyceps cicadae adenosine extracting process. The cordyceps cicadae adenosine preparation has the beneficial effects that the materials are easy to get, the extracting process is simple, and an extract is prepared into a pharmaceutically defined preparation with a conventional galenic pharmacy method.
Description
Technical field
The present invention relates to edible fungi field, particularly relate to a kind of Cordyceps cicadae Shing adenosine preparation and extraction process.
Background technology
Adenosine (Adenosine) is a kind of endogenous nucleoside spreading all over human body cell, can be directly entered cardiac muscle phosphorylated
Generate adenylic acid, participate in energy metabolism of myocardial, the most also participate in dilating coronary blood vessel, increase blood flow.Adenosine is to cardiovascular system
System and other system of many of human body and tissue all have physiological action.Adenosine is fast for synthesizing adenosine triphosphate (ATP), gland
Purine, adenylic acid, the important intermediate of vidarabine.Adenosine is endogenous purine nucleoside, can make atrioventricular node delayed conduction, blocks room
Room knot reentrant pathway, paroxysmal supraventricular tachycardia (PSVT) (accompany or do not accompany preexcitation syndrome) patient recovers the normal sinus heart
Rule.Adenosine can be absorbed by erythrocyte rapidly, and therefore action time is the shortest, and the plasma half-life of free adenosine is less than 10s.
Cordyceps cicadae Shing genetic machinery has good stability, and containing substantial amounts of adenosine, ergosterol, aminoacid, cordycepic acid, Cordyceps
Element, polysaccharide isoreactivity composition.Result of study shows that the cordycepic acid of Cordyceps cicadae Shing and adenosine content are above Cordyceps, polysaccharide and worm
Oxalic acid content is above Cordyceps militaris (L.) Link..Therefore from Cordyceps cicadae Shing, how to extract polysaccharide formulation become what those skilled in the art were badly in need of solving
Problem.
Summary of the invention
For the deficiencies in the prior art, an object of the present invention is to provide a kind of Cordyceps cicadae Shing adenosine preparation.
The two of the purpose of the present invention are to provide a kind of Cordyceps cicadae Shing adenosine extraction process.
In order to realize first goal of the invention, present invention employs techniques below scheme:
A kind of Cordyceps cicadae Shing adenosine preparation, is made up of the component of following material: Cordyceps cicadae Shing sporophore 10-30%, golden cicada beggar
Seat 70-90% and adjuvant 5%, three's component and be 100%.
In order to realize second goal of the invention, present invention employs techniques below scheme:
A kind of Cordyceps cicadae Shing adenosine extraction process, comprises the following steps:
1) Cordyceps cicadae Shing sporophore is proportionally crushed to after 300-2000 mesh with Cordyceps cicadae Shing Stroma mix；
2) by step 1) in obtain mixture acetone stirring immersion carry out ungrease treatment, after stratification collect third
Ketone；
3) to step 2) in the leftover materials vacuum drying that obtains obtain mixture except acetone；
4) by step 3) in the mixture that obtains and sodium dihydrogen phosphate extract in the ratio of 1:10-1:5 and filtered for 1-3 time
Liquid；
5) to step 4) filtrate that obtains carries out pressure filtration and ultrafiltration successively, then the filtrate obtained is concentrated into cream
Shape, and add adjuvant and make preparation.
Preferably, step 1) in Cordyceps cicadae Shing sporophore and Cordyceps cicadae Shing Stroma through dehumidifying, dried moisture Control
Below 5%, then pulverize with pulverizer, cross 300-2000 mesh sieve.
Preferably, step 2) in acetone consumption control through step 1) process the 5-10 of mixture obtained
Times, degreasing time controlled at 30-50 hour.
Preferably, step 3) in vacuum drying pressure be not less than 0.1Mpa, the vacuum drying time controls at 20-30
Hour.
Preferably, step 4) in Extracting temperature be 60-90 DEG C, extraction time is 20-30 hour.
Preferably, step 5) in carry out pressure filtration with 5-10 micron filter element, carry out the ultrafilter membrane molecule of ultrafiltration
Between amount is for 10000-50000, its thickening temperature controls below 90 DEG C.
Preferably, step 5) in adjuvant be HPC or CMC of 5%.
Preferably, step 5) preparation that obtains is granule, tablet or capsule.
The raw material of the present invention easy, extraction process simple, and extract made pharmaceutics by available conventional formulation method
Preparation in definition.
Detailed description of the invention
With instantiation, the present invention is described further below:
Embodiment 1:
A kind of Cordyceps cicadae Shing adenosine extraction process and preparation are prepared (granule), depletion Periostracum cicadae sporophore 100g, golden cicada beggar
Seat 900g, is crushed to 300 mesh respectively, first carries out ungrease treatment according to 5 times of acetone after mixing, and stirring is soaked 20 hours, stands and divides
Collecting acetone after Ceng, leftover materials carry out vacuum drying except acetone, 20 hours drying times, vacuum 0.1Mpa, vacuum drying
After carry out extracting 1 time according to powder and sodium dihydrogen phosphate (15%) 1:5 at 60 degrees Celsius by the powder of mixing, extraction time is 20 little
Time, carrying out pressure filtration with 5 microns of filter elements, filtrate carries out ultrafiltration again, and ultrafilter membrane molecular weight is 10000.The liquid that will collect
At a temperature of less than 90 DEG C, it is concentrated into paste, adds the HPC of 5%, mixing, carry out wet granulation with granulator, after having pelletized
In 60 degrees Celsius of baking ovens after drying, carry out packing according to 5g/ bag.
Embodiment 2:
A kind of Cordyceps cicadae Shing adenosine extraction process and preparation are prepared (tablet), depletion Periostracum cicadae sporophore 200g, Cordyceps cicadae Shing Stroma
800g, is crushed to 500 mesh respectively, first carries out ungrease treatment according to 7 times of acetone after mixing, and stirring is soaked 30 hours, stratification
Rear collection acetone, leftover materials carry out vacuum drying and remove acetone, and 30 hours drying times, vacuum 0.1Mpa, after vacuum drying
Carrying out extracting 3 times according to powder and sodium dihydrogen phosphate (15%) 1:10 at 60 degrees Celsius by the powder of mixing, extraction time is 30 little
Time, merging filtrate, carry out pressure filtration with 10 microns of filter elements, filtrate carries out ultrafiltration again, and ultrafilter membrane molecular weight is 50000.To receive
Collect to liquid at a temperature of less than 90 DEG C, be concentrated into paste, mixing, carry out wet granulation with granulator, 60 after having pelletized
In degree Celsius baking oven dried, carry out tabletting according to 2g/ sheet, according to 60/bottled bottle.
Embodiment 3:
A kind of Cordyceps cicadae Shing adenosine extraction process and preparation are prepared (capsule), depletion Periostracum cicadae sporophore 300g, golden cicada beggar
Seat 700g, is crushed to 10000 mesh respectively, first carries out ungrease treatment according to 10 times of acetone after mixing, and stirring is soaked 30 hours, stands
Collecting acetone after layering, leftover materials carry out vacuum drying and remove acetone, 30 hours drying times, vacuum 0.1Mpa, and vacuum is done
Carrying out extracting 3 times according to powder and sodium dihydrogen phosphate (15%) 1:10 at 60 degrees Celsius by the powder of mixing after dry, extraction time is
30 hours, merging filtrate, carry out pressure filtration with 10 microns of filter elements, filtrate carries out ultrafiltration again, and ultrafilter membrane molecular weight is 50000.
The liquid collected is concentrated into paste at a temperature of less than 90 DEG C, mixing, carry out wet granulation with granulator, after having pelletized
In 60 degrees Celsius of baking ovens after drying, fill is carried out according to 1g/ grain, according to 60/bottled bottle.
Claims (9)
1. a Cordyceps cicadae Shing adenosine preparation, it is characterised in that be made up of the component of following material:
Cordyceps cicadae Shing sporophore 10-30%
Cordyceps cicadae Shing Stroma 70-90%
Adjuvant 5%
Three's component and be 100%.
2. a Cordyceps cicadae Shing adenosine extraction process, it is characterised in that comprise the following steps:
1) Cordyceps cicadae Shing sporophore is proportionally crushed to after 300-2000 mesh with Cordyceps cicadae Shing Stroma mix；
2) by step 1) in obtain mixture acetone stirring immersion carry out ungrease treatment, after stratification collect acetone；
3) to step 2) in the leftover materials vacuum drying that obtains obtain mixture except acetone；
4) by step 3) in the mixture that obtains and sodium dihydrogen phosphate extract in the ratio of 1:10-1:5 and obtain filtrate 1-3 time；
5) to step 4) filtrate that obtains carries out pressure filtration and ultrafiltration successively, then the filtrate obtained is concentrated into paste, and
Add adjuvant and make preparation.
A kind of Cordyceps cicadae Shing adenosine extraction process the most according to claim 2, it is characterised in that step 1) in golden cicada beggar
Entity and Cordyceps cicadae Shing Stroma, are then pulverized with pulverizer through dehumidifying, dried moisture Control below 5%, cross 300-
2000 mesh sieves.
A kind of Cordyceps cicadae Shing adenosine extraction process the most according to claim 2, it is characterised in that step 2) in acetone consumption control
System is through step 1) process 5-10 times of mixture obtained, degreasing time controlled at 30-50 hour.
A kind of Cordyceps cicadae Shing adenosine extraction process the most according to claim 2, it is characterised in that step 3) middle vacuum drying pressure
Power is not less than 0.1Mpa, and the vacuum drying time controlled at 20-30 hour.
A kind of Cordyceps cicadae Shing adenosine extraction process the most according to claim 2, it is characterised in that step 4) in Extracting temperature
For 60-90 DEG C, extraction time is 20-30 hour.
A kind of Cordyceps cicadae Shing adenosine extraction process the most according to claim 2, it is characterised in that step 5) in use 5-10 micron
Filter element carries out pressure filtration, and the ultrafilter membrane molecular weight carrying out ultrafiltration is between 10000-50000, and its thickening temperature controls at 90 DEG C
Below.
A kind of Cordyceps cicadae Shing adenosine extraction process the most according to claim 2, it is characterised in that step 5) in adjuvant be 5%
HPC or CMC.
A kind of Cordyceps cicadae Shing adenosine extraction process the most according to claim 2, it is characterised in that step 5) preparation that obtains is
Granule, tablet or capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610687874.8A CN106317149B (en) | 2016-08-18 | 2016-08-18 | A kind of golden cicada flower adenosine preparation and extraction process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610687874.8A CN106317149B (en) | 2016-08-18 | 2016-08-18 | A kind of golden cicada flower adenosine preparation and extraction process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106317149A true CN106317149A (en) | 2017-01-11 |
| CN106317149B CN106317149B (en) | 2019-03-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610687874.8A Active CN106317149B (en) | 2016-08-18 | 2016-08-18 | A kind of golden cicada flower adenosine preparation and extraction process |
Country Status (1)
| Country | Link |
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| CN (1) | CN106317149B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113480587A (en) * | 2021-05-20 | 2021-10-08 | 徐州工程学院 | Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558265A (en) * | 2012-01-04 | 2012-07-11 | 正源堂（天津）生物科技有限公司 | Extract method of cordyceps militaris adenosine |
| CN102824376A (en) * | 2012-09-17 | 2012-12-19 | 张连龙 | Cordyceps sinensis powder and preparation method and application thereof |
| CN104490941A (en) * | 2014-12-17 | 2015-04-08 | 李豫杰 | Cordyceps cicadae extract improving immunity, resisting tumor, improving renal function and promoting sleep |
-
2016
- 2016-08-18 CN CN201610687874.8A patent/CN106317149B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558265A (en) * | 2012-01-04 | 2012-07-11 | 正源堂（天津）生物科技有限公司 | Extract method of cordyceps militaris adenosine |
| CN102824376A (en) * | 2012-09-17 | 2012-12-19 | 张连龙 | Cordyceps sinensis powder and preparation method and application thereof |
| CN104490941A (en) * | 2014-12-17 | 2015-04-08 | 李豫杰 | Cordyceps cicadae extract improving immunity, resisting tumor, improving renal function and promoting sleep |
Non-Patent Citations (3)
| Title |
|---|
| 宋玉良，等: "高效液相色谱法测定蝉花中腺苷的含量", 《中药新药与临床药理》 * |
| 李明波，等: "超声提取蝉花中核苷碱基的工艺条件", 《贵州农业科学》 * |
| 钱正明，等: "高效液相色谱定量分析比较4种虫草药材的核苷类成分", 《中国中药杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113480587A (en) * | 2021-05-20 | 2021-10-08 | 徐州工程学院 | Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera sporocarp |
| CN113480587B (en) * | 2021-05-20 | 2024-05-03 | 湖南合健生物科技有限公司 | Method for efficiently extracting N6- (2-hydroxyethyl) adenosine from cordyceps sobolifera fruiting bodies |
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| CN106317149B (en) | 2019-03-22 |
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