CN106311369A

CN106311369A - Anti-pollution pipette tip

Info

Publication number: CN106311369A
Application number: CN201610896046.5A
Authority: CN
Inventors: 何农跃; 王超; 肖鹏峰; 朱禾; 胡雅娜
Original assignee: Southeast University
Current assignee: Southeast University
Priority date: 2016-10-13
Filing date: 2016-10-13
Publication date: 2017-01-11

Abstract

The invention discloses an anti-pollution pipette tip. The anti-pollution pipette tip is provided with an inner chamber and an outer chamber, wherein the upper end of the inner chamber is closed, an elastic membrane is arranged on the side wall of the upper half part, the lower half part is used for storing sucked liquid, an opening is formed in the lower end of the inner chamber and is used for sucking and discharging liquid, an opening of the upper end of the outer chamber is jointed with a pipettor pipeline, a lower port is closed, and the inner wall is the side wall of the inner chamber. In the process of sucking and discharging process, liquid is always in the inner chamber, and the pipettor pipeline is jointed with the outer chamber and is isolated from the inner chamber, so that the liquid only can stay in the inner chamber even if the liquid is excessively sucked or the gunpoint inclines, volatile substances in the liquid can also stay in the inner chamber and can not enter the pipettor pipeline, and the pipettor pipeline is not polluted by the liquid in the pipette tip.

Description

A kind of anti-pollution liquid-transfering gun point

Technical field

The present invention relates to a kind of anti-pollution liquid-transfering gun point, it is adaptable to such as biotechnology, medical test, chemical analysis etc. need Pipettor to be used carries out drawing and discharging the technical field of liquid.

Background technology

PCR amplification technique is widely used in the fields such as biology, chemistry, medical science, typically section of DNA fragment is passed through PCR instrument Expand, then by pipettor and rifle point transfer product to agarose gel electrophoresis instrument carry out experimental judgment DNA fragmentation be No Successful amplification, owing to, in the PCR pipe after PCR expands, DNA fragmentation concentration is the highest, shifts with pipettor and rifle point and expands The when of volume increase thing, during in rifle point, the DNA fragmentation of product can escape into pipettor pipeline, expand if shifting other with this pipettor again The when of volume increase thing, it is trapped in the DNA fragmentation in pipettor pipeline before and may mix in the product, cause false positive, institute Must assure that pipettor pipeline is not contaminated in testing with this type of.

Liquid-transfering gun point is that device is commonly used in the field such as biology, chemistry, medical science, agronomy, coordinates pipettor can realize micro liquid Accurately draw and discharge, either manual experiment or automatic fluid work station all play important role.The most often Liquid-transfering gun point tapered, upper and lower side is respectively arranged with an opening, and upper end open is used for more greatly connecting pipettor pipeline, lower ending opening Less, for the turnover of liquid, drawing liquid when, owing to the upper end of rifle point is directly connected to pipettor pipeline, liquid In steam or other volatile substances can be entered in pipettor pipeline by rifle point, when going again with this pipettor assembling rifle point When drawing other liquid, being detained other materials evaporated out in pipettor pipeline, these materials may be with other liquid Body mixes, and pollutes liquid, and it is internal near place, top placement filter membrane at rifle point, only for preventing this type of common method polluted at present Can be by the material suitable with the molecular size in air, this method is fairly simple, uses extensively, but has two problems: 1. the material molecule size that some evaporates out is little with the molecular difference in air, again may be by filter membrane；If 2. Running into that the liquid draw scale of construction is relatively big or the situation such as rifle point inclination, liquid may pass through filter element, is directly entered pipettor pipeline.

Summary of the invention

It is an object of the present invention to provide a kind of anti-pollution liquid-transfering gun point, can effectively prevent in the liquid in liquid-transfering gun point and liquid Volatile substance enter in pipettor pipeline, produce and pollute.

A technical scheme according to the present invention, a kind of anti-pollution liquid-transfering gun point, including rifle point, rifle point be divided into inner cavity chamber with Exocoel, inner cavity chamber half embeds in exocoel, and inner cavity chamber is provided with elastica, inner cavity chamber top in the partial sidewall embedding exocoel Closing, lower end is provided with imbibition and leakage fluid dram, and exocoel top is provided with rifle point and pipettor pipeline connection port.

Described exocoel is cylindrical.

Described inner cavity chamber bottom is cone.

Beneficial effect

The anti-pollution liquid-transfering gun point that the present invention proposes, this rifle point has two chambers, and liquid is deposited by inner cavity chamber, exocoel with Pipettor pipeline connects, owing to interior exocoel does not connects, so pipettor pipeline does not contacts with liquid, in liquid and liquid The material of volatilization can only stay inner cavity chamber, will not pollute pipettor pipeline, so again drawing other with this pipettor This liquid mixing also will not be polluted by liquid.

Owing to the top half sidewall of inner cavity chamber is elastica, when applying pressure to sidewall, elastica produces deformation, pressure After cancelling, elastica is recovered, and pipettor draws liquid by elastica is applied pressure.

Accompanying drawing explanation

Fig. 1 anti-pollution rifle point front view；

Fig. 2 anti-pollution rifle point sectional view；

Wherein: 1 exocoel, 2 inner cavity chamber, 21 rifle points and pipettor pipeline connection port, 22 elasticas, 23 imbibitions and discharge opeing Mouthful.

Detailed description of the invention

The present invention done further explain below in conjunction with pcr amplification product transfer embodiment and accompanying drawing.Embodiment is only For the present invention is described, but it is not used to limit the practical range of the present invention.

A kind of anti-pollution liquid-transfering gun point, has inside and outside two chambers, inner cavity chamber's upper end closed, and top half sidewall is elastic Film, when applying pressure to sidewall, elastica produces deformation, and after pressure is cancelled, elastica is recovered, and there is opening inner cavity chamber lower end, Inserting below liquid level, draw liquid, leave inner cavity chamber the latter half in, exocoel upper end open engages with pipettor pipeline, under Port closed, inwall is inner cavity chamber's sidewall, and when drawing liquid when, pipettor injects gas in exocoel, in exocoel Portion's air pressure raises, and on inwall, elastica is oppressed, inward deflection, and rifle point lower end is now inserted into below liquid level, and pipettor will From inner cavity chamber's draw gas, elastica is recovered, and inner cavity chamber's air pressure diminishes, less than liquid levels air pressure, owing to pressure reduction exists, liquid Being sucked in rifle point, when rifle point internal liquid discharged by needs, pipettor injects gas to exocoel, and elastica is oppressed, to Bias internal, rifle point air pressure inside uprises, and more than external pressure, liquid is discharged, and completes imbibition and discharge opeing process, in whole process In, liquid is all the time in inner cavity chamber, and pipettor pipeline engages with exocoel, completely cuts off with inner cavity chamber, even if liquid assimilating is too much Or rifle point tilts, and liquid only can rest in inner cavity chamber, volatilizes material and can only rest in inner cavity chamber in same liquid, nothing Method enters in pipettor pipeline.

Embodiment

Anti-pollution liquid-transfering gun point top half in the present invention is cylindrical, and the latter half is cone, mainly has two Part composition: exocoel 1, inner cavity chamber 2.Exocoel 1 upper end open is rifle point and pipettor pipe joint 21, rounded, interior table Can combine closely with pipettor pipeline external surface in face, flexible film 22 in the middle of exocoel 1 inwall, exocoel 1 internal gas pressure becomes During change, elastica 22 can deform upon accordingly, and inner cavity chamber 2 top half sidewall is isolated by elastica 22 with exocoel 1, inner chamber The latter half, room 2 is tapered, and for depositing the liquid of absorption, bottom opening is imbibition and leakage fluid dram 23, inhales when drawing liquid Liquid and leakage fluid dram 23 need to be inserted into below liquid level.Before PCR primer shifts, by pipettor pipeline and rifle point and pipettor pipe Pipeline joint 21 is combined closely, and presses pipettor operation button, and in exocoel 1, air pressure raises, and elastica 22 produces deformation, to convex Go out, then open be complete amplification have product PCR pipe, imbibition and the leakage fluid dram 23 of rifle point are inserted in PCR pipe Below product level, unclamping pipettor operation button, in exocoel 1, air pressure recovers, and elastica 22, due to elastic reaction, recovers former Shape, now in inner cavity chamber, air pressure declines, owing to above liquid level in PCR pipe, air pressure enters inner cavity chamber 2 more than inner cavity chamber's air pressure, liquid In, move to pipettor and rifle point be loaded on agarose gel, first imbibition and leakage fluid dram 23 are inserted gel, then by moving down liquid Device operation button, exocoel 1 internal gas pressure raises, and compressing elastica 22 is protruded to inner cavity chamber 2, and in inner cavity chamber 2, air pressure raises, and is more than Rifle point external pressure, product is got rid of to gel, is added buffer, after mixing with product, carry out electrophoresis experiment.Whole PCR In product transfer process, product is drawn and is left in inner cavity chamber, and the pipeline of pipettor engages with exocoel, product and product The DNA fragmentation of middle effusion cannot be introduced in pipettor pipeline, it is ensured that during whole, pipettor pipeline is not contaminated, Bu Huiying Ring next time and move liquid.

Claims

1. an anti-pollution liquid-transfering gun point, including rifle point, it is characterised in that rifle point is divided into inner cavity chamber and exocoel, inner cavity chamber half Embedding in exocoel, inner cavity chamber is provided with elastica, inner cavity chamber's top closure in the partial sidewall embedding exocoel, and lower end is provided with suction Liquid and leakage fluid dram, exocoel top is provided with rifle point and pipettor pipeline connection port.

Anti-pollution liquid-transfering gun the most according to claim 1 point, it is characterised in that: described exocoel is cylindrical.

Anti-pollution liquid-transfering gun the most according to claim 1 point, it is characterised in that: described inner cavity chamber bottom is cone.