CN106306412A - High protein enrichment method of vinasse - Google Patents
High protein enrichment method of vinasse Download PDFInfo
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- CN106306412A CN106306412A CN201610687988.2A CN201610687988A CN106306412A CN 106306412 A CN106306412 A CN 106306412A CN 201610687988 A CN201610687988 A CN 201610687988A CN 106306412 A CN106306412 A CN 106306412A
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- powder
- distiller grains
- vinasse
- high protein
- enrichment method
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A high protein enrichment method of vinasse comprises the following steps: 1) putting fresh vinasse into a vacuum low-temperature cold air dryer, and drying in a 40-45 DEG C environment till the moisture content of the vinasse is 46-48%; 2) crushing vinasse after cold air drying, sieving by using a 60-80-mesh medicinal sieve to obtain wet powder, sending the wet powder into microwave processing equipment, and performing intermittent microwave treatment; 3) adding bran into the wet powder after the microwave treatment to obtain a mixed medium; 4) regulating the moisture content of the mixed medium to 35% by using a nutrient salt solution, regulating the pH value to 5.5, then adding 1-3% of fresh pig bone meal, 0.3% of Daqu powder, 0.2% of potassium dihydrogen phosphate and 0.2% of selenium-enriched yeast in terms of the weight of the mixed medium, mixing, and sterilizing to obtain a fermentation medium; 5) inoculating the fermentation medium with a mixed culture, keeping the temperature at 30-40 DEG C and culturing for 3-8 days in a constant-temperature incubator to obtain high-protein crude vinasse; 6) sending the high-protein crude vinasse into a grinding machine, continuously grinding for 2 hours, drying at 50-55 DEG C, and then crushing to obtain high-protein vinasse powder.
Description
Technical field
The present invention relates to technical field of bioengineering, be specifically related to a kind of distiller grains high protein enrichment method.
Background technology
Distillers ' grains is the waste residue or by-product produced in liquor production, and it processes and utilizes the weight having become as industry work
Point.China is liquor production and consumption big country, and Chinese liquor yield is the abundantest, and according to statistics, the annual distillers ' grains yield of China is up to
25000000 tons, distillers ' grains has the characteristic of highly acidity, high-moisture, as processed not in time, will go bad, cause environmental pollution, with
Containing abundant nutritional labeling in Shi Yinwei distillers ' grains, such as because the part material that saccharifying, fermentation are the most thorough and remaining is remaining
Thing, also thalline and metabolism product thereof and thalline autolysate, therefore it is that one well can probe into resource.If in time
Utilize distillers ' grains, then can solve environmental problem, obtain again significant economic benefit.Therefore the distillers ' grains process side of reasonable
Formula is to solve environmental crisis and improve the effective way of Business Economic Benefit.
Containing certain crude protein, crude fibre, crude fat and nitrogen-free extract, rich in nutrition content in distiller grains.Additionally, wine
In grain, amino acid classes is complete, comprises 8 kinds of essential amino acids, possibly together with multivitamin (as VA, vitamin B group, VC, VE,
VPP, nicotiamide etc.) and the mineral such as calcium, phosphorus, potassium.And containing tunnings such as quite abundant alcohol, ester and acid in distiller grains,
And produced by microbial cells ribonucleic acid, purine, pyrimidine, the trace beneficiating ingredient such as lipids.The most apparently, wine
Grain has the highest value of exploiting and utilizing.
China is the country of protein resource famine, and arable land of having a large population is few, today that industrial proteins resource is day by day deficient,
Develop protein resource and there is the strongest realistic meaning and using value.
Summary of the invention
The technical problem to be solved is to provide a kind of high financial profit, the distiller grains height egg that end product quality is stable
White enrichment method.
The technical problem to be solved realizes by the following technical solutions:
A kind of distiller grains high protein enrichment method, concrete grammar step is as follows:
(1) fresh grain stillage is placed in vacuum and low temperature cold wind drier, is dried to distiller grains moisture under 40-45 DEG C of environment
At 46-48%；
Because distiller grains albumen, fat content are enriched, as fat oxidation caused to affect product quality with conventional hot air drying,
Therefore we select vacuum and low temperature cold air drying technology to be dried process, so that product keeps preferable stability.It is dried temperature
Degree and time are the optimal parameters obtained in substantial amounts of experimental basis, the product with stable quality obtained under the conditions of this, and Yi work
Industry metaplasia is produced.
(2) distiller grains after cold air drying are pulverized, cross 60～80 mesh medicine sieves, obtain moistening powder, by moistening powder
Sending in microwave processing equipment, be spaced microwave treatment under microwave frequency 2450MHz, power 500W, interval time is 5 minutes,
Microwave treatment 10 minutes, is carried out continuously 3-4 time every time；
(3) adding wheat bran in moistening powder after the microwave treatment, the addition of wheat bran is the 5-of moistening powder weight
8%, stir, obtain mixed-matrix；
(4) with nutrition saline solution, the moisture of mixed-matrix being adjusted to about 35%, adjusting pH value is about 5.5, then adds
Enter the Fresh Os Sus domestica powder of mixed-matrix (moisture 35%) weight 1-3%, the koji powder of 0.3%, the potassium dihydrogen phosphate of 0.2% and
The yeast rich in selenium of 0.2%, carries out sterilization treatment, obtains fermentation medium after mixing；
(5) inoculating mixed bacteria in fermentation medium, the inoculum concentration of described mixed bacteria is 5%, at constant incubator
Interior holding 30-40 DEG C is cultivated 3 days-8 days, obtains the thick distiller grains of high protein；
Described mixed bacteria is papain, Trichoderma viride, healthy and free from worry trichoderma, candida tropicalis and bacillus subtilis
The mixture of the equal-volume ratio that bacterium is the most several.
(6) thick for high protein distiller grains are sent into grinder, continuously grinding 2 hours, cross 90-120 mesh sieve, in 50-55 DEG C of drying,
Pulverize again and obtain high protein distiller grains powder.
Described nutrition saline solution is that the component by following weight is formulated: dipotassium hydrogen phosphate 5g, SHENGXIANG yeast 2g, ammonium sulfate
2g, magnesium sulfate 3g, dehydrated alcohol 5g, magnesium phosphate 2g, sodium chloride 10g, pure water 100g.
The invention has the beneficial effects as follows: the high protein distiller grains that the present invention obtains, crude protein content is up to 42%, far above it
The 25-33% of its document report；It is applied widely, can add in the feedstuff of the animals such as pig, cattle, chicken, duck, fish, is a kind of
High-quality and efficient feedstuff fermentable goods.And energy consumption is low；Low cost, with short production cycle, production efficiency is high；Can reach money
Source regeneration, reduces the discharge of pollutant, beneficially built environment friendly, conservation-minded society.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below knot
Close specific embodiment, the present invention is expanded on further.
Embodiment 1
A kind of distiller grains high protein enrichment method, concrete grammar step is as follows:
(1) fresh grain stillage is placed in vacuum and low temperature cold wind drier, is dried to distiller grains moisture under 40-45 DEG C of environment
At 46-48%；
Because distiller grains albumen, fat content are enriched, as fat oxidation caused to affect product quality with conventional hot air drying,
Therefore we select vacuum and low temperature cold air drying technology to be dried process, so that product keeps preferable stability.It is dried temperature
Degree and time are the optimal parameters obtained in substantial amounts of experimental basis, the product with stable quality obtained under the conditions of this, and Yi work
Industry metaplasia is produced.
(2) distiller grains after cold air drying are pulverized, cross 60～80 mesh medicine sieves, obtain moistening powder, by moistening powder
Sending in microwave processing equipment, be spaced microwave treatment under microwave frequency 2450MHz, power 500W, interval time is 5 minutes,
Microwave treatment 10 minutes, is carried out continuously 3-4 time every time；
(3) adding wheat bran in moistening powder after the microwave treatment, the addition of wheat bran is the 5-of moistening powder weight
8%, stir, obtain mixed-matrix；
(4) with nutrition saline solution, the moisture of mixed-matrix being adjusted to about 35%, adjusting pH value is about 5.5, then adds
Enter the Fresh Os Sus domestica powder of mixed-matrix (moisture 35%) weight 1-3%, the koji powder of 0.3%, the potassium dihydrogen phosphate of 0.2% and
The yeast rich in selenium of 0.2%, carries out sterilization treatment, obtains fermentation medium after mixing；
(5) inoculating mixed bacteria in fermentation medium, the inoculum concentration of described mixed bacteria is 5%, at constant incubator
Interior holding 40 DEG C is cultivated 3 days, obtains the thick distiller grains of high protein；
Described mixed bacteria is papain, Trichoderma viride, healthy and free from worry trichoderma, candida tropicalis and bacillus subtilis
The mixture of the equal-volume ratio that bacterium is the most several.
(6) thick for high protein distiller grains are sent into grinder, continuously grinding 2 hours, cross 90-120 mesh sieve, in 50-55 DEG C of drying,
Pulverize again and obtain high protein distiller grains powder.
Embodiment 2
A kind of distiller grains high protein enrichment method, concrete grammar step is as follows:
(1) fresh grain stillage is placed in vacuum and low temperature cold wind drier, is dried to distiller grains moisture under 40-45 DEG C of environment
At 46-48%；
Because distiller grains albumen, fat content are enriched, as fat oxidation caused to affect product quality with conventional hot air drying,
Therefore we select vacuum and low temperature cold air drying technology to be dried process, so that product keeps preferable stability.It is dried temperature
Degree and time are the optimal parameters obtained in substantial amounts of experimental basis, the product with stable quality obtained under the conditions of this, and Yi work
Industry metaplasia is produced.
(2) distiller grains after cold air drying are pulverized, cross 60～80 mesh medicine sieves, obtain moistening powder, by moistening powder
Sending in microwave processing equipment, be spaced microwave treatment under microwave frequency 2450MHz, power 500W, interval time is 5 minutes,
Microwave treatment 10 minutes, is carried out continuously 3-4 time every time；
(3) adding wheat bran in moistening powder after the microwave treatment, the addition of wheat bran is the 5-of moistening powder weight
8%, stir, obtain mixed-matrix；
(4) with nutrition saline solution, the moisture of mixed-matrix being adjusted to about 35%, adjusting pH value is about 5.5, then adds
Enter the Fresh Os Sus domestica powder of mixed-matrix (moisture 35%) weight 1-3%, the koji powder of 0.3%, the potassium dihydrogen phosphate of 0.2% and
The yeast rich in selenium of 0.2%, carries out sterilization treatment, obtains fermentation medium after mixing；
(5) inoculating mixed bacteria in fermentation medium, the inoculum concentration of described mixed bacteria is 5%, at constant incubator
Interior holding 35 DEG C is cultivated 5 days, obtains the thick distiller grains of high protein；
Described mixed bacteria is papain, Trichoderma viride, healthy and free from worry trichoderma, candida tropicalis and bacillus subtilis
The mixture of the equal-volume ratio that bacterium is the most several.
(6) thick for high protein distiller grains are sent into grinder, continuously grinding 2 hours, cross 90-120 mesh sieve, in 50-55 DEG C of drying,
Pulverize again and obtain high protein distiller grains powder.
Described nutrition saline solution is that the component by following weight is formulated: dipotassium hydrogen phosphate 5g, SHENGXIANG yeast 2g, ammonium sulfate
2g, magnesium sulfate 3g, dehydrated alcohol 5g, magnesium phosphate 2g, sodium chloride 10g, pure water 100g.
The product coarse protein content that the present embodiment obtains is 43%, and aminoacid forms and content is: lysine 3.04%, Soviet Union
Propylhomoserin 1.12%, phenylalanine 1.3%, methionine 0.9%, tryptophan 2.8%, histidine 0.4%, leucine 3.22%, figured silk fabrics
Propylhomoserin 1.72%, isoleucine 0.97%, arginine 1.76%, cystine 0.91%, glycine 1.31%, tyrosine
0.93%, aspartic acid 2.72%, proline 2.44%, serine 1.96%, glutamic acid 8.49%, alanine 2.72%.This
Embodiment product coarse fiber content is 17.3%.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.The technology of the industry
Personnel, it should be appreciated that the present invention is not restricted to the described embodiments, simply illustrating this described in above-described embodiment and description
The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, and these become
Change and improvement both falls within scope of the claimed invention.Claimed scope by appending claims and
Equivalent defines.
Claims (5)
1. a distiller grains high protein enrichment method, it is characterised in that concrete grammar step is as follows:
(1) fresh grain stillage is placed in vacuum and low temperature cold wind drier, is dried under 40-45 DEG C of environment to distiller grains moisture and exists
46-48%；
(2) distiller grains after cold air drying are pulverized, cross 60～80 mesh medicine sieves, obtain moistening powder, moistening powder is sent into
In microwave processing equipment, being spaced microwave treatment under microwave frequency 2450MHz, power 500W, interval time is 5 minutes, every time
Microwave treatment 10 minutes, is carried out continuously 3-4 time；
(3) adding wheat bran in moistening powder after the microwave treatment, the addition of wheat bran is the 5-8% of moistening powder weight, stirs
Mix uniformly, obtain mixed-matrix；
(4) with nutrition saline solution, the moisture of mixed-matrix being adjusted to 35%, adjusting pH value is 5.5, adds mixed-matrix weight
The amount Fresh Os Sus domestica powder of 1-3%, the koji powder of 0.3%, the potassium dihydrogen phosphate of 0.2% and the yeast rich in selenium of 0.2%, carried out after mixing
Sterilization treatment, obtains fermentation medium；
(5) inoculating mixed bacteria in fermentation medium, the inoculum concentration of described mixed bacteria is 5%, protects in constant incubator
Hold 30-40 DEG C to cultivate 3 days-8 days, obtain the thick distiller grains of high protein；
(6) thick for high protein distiller grains are sent into grinder, continuously grinding 2 hours, cross 90-120 mesh sieve, in 50-55 DEG C of drying, then powder
Broken obtain high protein distiller grains powder.
A kind of distiller grains high protein enrichment method the most according to claim 1, it is characterised in that described mixed bacteria is Fructus Chaenomelis
The mixing of the equal-volume ratio that protease, Trichoderma viride, healthy and free from worry trichoderma, candida tropicalis and bacillus subtilis are the most several
Thing.
A kind of distiller grains high protein enrichment method the most according to claim 1, it is characterised in that described nutrition saline solution be by with
The component of lower weight is formulated: dipotassium hydrogen phosphate 5g, SHENGXIANG yeast 2g, ammonium sulfate 2g, magnesium sulfate 3g, dehydrated alcohol 5g, phosphorus
Acid magnesium 2g, sodium chloride 10g, pure water 100g.
A kind of distiller grains high protein enrichment method the most according to claim 1, it is characterised in that keep in constant incubator
Cultivate 5 days for 35 DEG C.
A kind of distiller grains high protein enrichment method the most according to claim 1, it is characterised in that keep in constant incubator
Cultivate 3 days for 40 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610687988.2A CN106306412A (en) | 2016-08-18 | 2016-08-18 | High protein enrichment method of vinasse |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610687988.2A CN106306412A (en) | 2016-08-18 | 2016-08-18 | High protein enrichment method of vinasse |
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| CN106306412A true CN106306412A (en) | 2017-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610687988.2A Pending CN106306412A (en) | 2016-08-18 | 2016-08-18 | High protein enrichment method of vinasse |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007320A (en) * | 2018-06-13 | 2018-12-18 | 马鞍山中粮生物化学有限公司 | A kind of maize alcohol stillage feed and preparation method thereof |
| CN116037602A (en) * | 2022-11-16 | 2023-05-02 | 布勒（无锡）商业有限公司 | Comprehensive utilization method of distilled grains of white spirit |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1285156A (en) * | 1999-08-23 | 2001-02-28 | 中国环境科学研究院 | Technology for producing vinasse somatic protein fodder by solide aerobic fermentation |
| CN104171265A (en) * | 2014-07-09 | 2014-12-03 | 四川农业大学 | Processing technology of single-cell protein from distilled spirit lees |
| CN104431295A (en) * | 2014-12-19 | 2015-03-25 | 四川省食品发酵工业研究设计院 | Method for preparing livestock feed from vinasse |
-
2016
- 2016-08-18 CN CN201610687988.2A patent/CN106306412A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1285156A (en) * | 1999-08-23 | 2001-02-28 | 中国环境科学研究院 | Technology for producing vinasse somatic protein fodder by solide aerobic fermentation |
| CN104171265A (en) * | 2014-07-09 | 2014-12-03 | 四川农业大学 | Processing technology of single-cell protein from distilled spirit lees |
| CN104431295A (en) * | 2014-12-19 | 2015-03-25 | 四川省食品发酵工业研究设计院 | Method for preparing livestock feed from vinasse |
Non-Patent Citations (2)
| Title |
|---|
| 李爱科: "《中国蛋白质饲料资源》", 31 January 2013, 中国农业大学出版社 * |
| 石旭东: "《养猪实用技术》", 31 May 2012, 甘肃科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109007320A (en) * | 2018-06-13 | 2018-12-18 | 马鞍山中粮生物化学有限公司 | A kind of maize alcohol stillage feed and preparation method thereof |
| CN116037602A (en) * | 2022-11-16 | 2023-05-02 | 布勒（无锡）商业有限公司 | Comprehensive utilization method of distilled grains of white spirit |
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Application publication date: 20170111 |
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