CN106306378A - Chelated iron compound feed and preparation method thereof - Google Patents
Chelated iron compound feed and preparation method thereof Download PDFInfo
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- CN106306378A CN106306378A CN201610687046.4A CN201610687046A CN106306378A CN 106306378 A CN106306378 A CN 106306378A CN 201610687046 A CN201610687046 A CN 201610687046A CN 106306378 A CN106306378 A CN 106306378A
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Abstract
The invention relates to chelated iron compound feed and a preparation method thereof. The chelated iron compound feed is prepared from the following raw materials in parts by weight: 20-30 parts of defatted rice bran, 10-20 parts of corn flour, 10-15 parts of sweet potato vine powder, 10-20 parts of wheat bran, 15-30 parts of soybean cake flour, 30-40 parts of sea cucumber intestine extract chelated iron and 4-6 parts of bacillus subtilis, wherein a preparation method of the sea cucumber intestine extract chelated iron comprises the following steps: (1) preprocessing sea cucumber intestines; (2) homogenizing the sea cucumber intestines; (3) performing primary enzymolysis; (4) performing secondary enzymolysis; (5) performing ultrafiltration; (6) performing nanofiltration; (7) chelating. The digestion-promoting and growth-helping chelated iron compound feed prepared by the preparation method provided by the invention is rich in nutrients, easy to absorb and use, high in absorption ratio and utilization ratio, low in impurity content and heavy metal content and high in nutritional value, and can supplement an iron element as well as a variety of nutrient elements from the sea cucumber intestines.
Description
Technical field
The present invention relates to a kind of chelated iron mixed feed and preparation method thereof.
Background technology
Stichopus japonicus belongs to one of precious seafood, and it is internal contains more than the 50 kind of nutritional labeling useful to human physiological activity, wherein
Protein content is higher, trace element abundant species, it is possible to continuity human senility, tonifies Qi of the kidney, essence-replenishing and marrow-strengthening, allaying tiredness,
Have more anticoagulation, antitumor, antibacterial, antiviral and improve the effect of immunity.In recent years, the enterprise of processing sea cucumber the most more comes
The most, contained in the leftover bits and pieces-Intestinum Stichopi japonici during Holothurian machining nutritional labelings, no less than body wall.Its dry intestinal rate Han vanadium
It is 12 parts of million parts, higher than the rate Han vanadium in its body 3 times.It has effect of warming middle-Jiao and tonifying deficiency pain relieving, can treat stomach and ten
Two Duodenalulcers.
The utilization of Intestinum Stichopi japonici resource day by day causes attention, after Intestinum Stichopi japonici is manually removed sand by a lot of enterprises, cleans, cold air drying
Dry or be spray-dried, then polishing or micronizing, make capsule product.But Intestinum Stichopi japonici is not done life by this kind of capsule product
Thing processes, and absorption rate is low, and nutritive value is had a greatly reduced quality.It addition, there is also in the process of raw material: manually going of Intestinum Stichopi japonici
Husky mud efficiency is low and silt is thorough, thorough with water cleaning, desalting, causes what content of beary metal and salinity in product exceeded standard to ask
Topic.
The elements such as iodine, ferrum, phosphorus are the indispensable elements of animal body, Deficiency of Intake, can affect animal body healthy, chelating amino acids
Ferrum is a kind of iron construction form that iron ion is entrenched in the middle of two amino acid moleculars.Two amino acid moleculars are as " crab claw "
Clamp an iron ion, form overstable chelate structure, then by aminoacid passage, Railway transportation is delivered in blood, make ferrum and ammonia
Base acid is absorbed by animal body together, can be greatly improved absorbance, and Intestinum Stichopi japonici is extracted and obtains extract chelated iron, then by the present invention
Mix with other adjuvant, make chelated iron mixed feed, have iron supplement, iodine and phosphorus function concurrently, it is also possible to be efficient supplementary multiple next
From the nutrient in Intestinum Stichopi japonici, animal body absorption rate is high, it is possible to promote animal body digestion and growth.
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, improve a kind of chelated iron mixed feed and preparation side thereof
Method, chelated iron mixed feed rich in nutrition content prepared by the method, it is easy to absorbing, impurity and content of beary metal are low, hold concurrently
Tool iron supplement, iodine and phosphorus function, it is also possible to the efficient supplementary multiple nutrient come from Intestinum Stichopi japonici, animal body absorbs
Rate is high, it is possible to promote animal body digestion and growth.
The technical solution adopted for the present invention to solve the technical problems is:
Chelated iron mixed feed, described chelated iron mixed feed is formed by the Raw material processing of following weight portion: defatted rice bran
20-30 part, Semen Maydis powder 10～20 parts, sweet potato vine powder 10～15 parts, Testa Tritici 10-20 part, soybean cake powder 15-30 part, Intestinum Stichopi japonici extracts
Thing chelated iron 30-40 part, bacillus subtilis 4-6 part, the preparation method of described Intestinum Stichopi japonici extract chelated iron includes walking as follows
Rapid:
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, pretreating agent A
For the water-insoluble glucan suspension of mass fraction 8-12%, described pretreating agent B is the reduction of mass fraction 0.5-1.5%
Type glutathion aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is
1:2-3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or
Pretreating agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, under room temperature at stirring
Reason 2-2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
Rich in silt, heavy metal, salinity in Intestinum Stichopi japonici, want to obtain the Intestinum Stichopi japonici extract chelated iron of high-quality, need
Fully removing above-mentioned impurity, the design concept of the present invention is as follows: (1) water-insoluble glucan suspension is the water suspendible of macromole
Liquid, macromole glucosan itself has the effect of flocculation of reuniting, and can fully adsorb or the mud that flocculates in Intestinum Stichopi japonici and heavy metal
The removal effect of the impurity such as impurity, especially heavy metal is splendid, after using glucosan suspension pretreating agent pretreatment, and Stichopus japonicus
The extensibility of intestinal is the most excellent, and follow-up homogenizing, the effect of enzymolysis are substantially improved, it addition, glucosan safety non-toxic, the glucosan of residual
There is the effect increasing immunity, improving immunologic function on the contrary, improve the nutritive value of Intestinum Stichopi japonici extract chelated iron；(2) reduction
Type glutathion aqueous solution has the function of good activating cell and tissue, can improve active oxygen in cell or tissue and
Osmotic pressure, maintains Intestinum Stichopi japonici cell and the fresh and healthy state of tissue long period, is substantially improved the effect of follow-up ferment treatment, paddy
Guang sweet peptide also safety non-toxic, the glutathion of residual has the function improving immune system, removing toxic substances on the contrary, improves Intestinum Stichopi japonici extract
The nutritive value of chelated iron, improves the quality of finished product, by Heavy Metal Pollution wind that may be present in Intestinum Stichopi japonici extract chelated iron
Danger reduces further or eliminates；It addition, the reduced glutathion of residual plays the effect of antioxidant, chelated iron mixed feed
Steady quality, long shelf-life, edible safety；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer, remove the fat in Intestinum Stichopi japonici by lipase enzymolysis
Fat, can improve the quality of Intestinum Stichopi japonici extract chelated iron, extends the shelf-life of Intestinum Stichopi japonici extract chelated iron；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant, logical
Cross enzymolysis and the protein in Intestinum Stichopi japonici is fully degraded into aminoacid and micromolecule polypeptide, it is simple to Intestinum Stichopi japonici extract chelated iron
Absorbing and utilize, utilization rate is high, is of high nutritive value；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is for 150-300Da, by nanofiltration to Intestinum Stichopi japonici extract chelated iron desalting processing, it is thus achieved that Intestinum Stichopi japonici extract chelating iron salt
Divide low, in good taste；
(7) chelating: under conditions of 35-45 DEG C, add appropriate ferrous salt in nanofiltration concentrated solution while stirring, delay simultaneously
Slow appropriate amount of acid regulation system pH that adds is 3-4, and the response time is 2～3h, maintenance system pH stable in course of reaction, reaction knot
Cooling down after bundle, filter, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated iron.
Described ferrous salt is ferrous sulfate and/or ferrous chloride.
Preferably, defatted rice bran 25 parts, Semen Maydis powder 15 parts, 12 parts of sweet potato vine powder, 15 parts of Testa Tritici, soybean cake powder 25 parts, Stichopus japonicus
Intestinal extract chelated iron 35 parts, bacillus subtilis 5 parts.
Preferably, in step (1), pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, described pre-
Inorganic agent B is the reduced glutathion aqueous solution of mass fraction 1.0%.
Preferably, temperature 35-65 DEG C of step (2) mesohigh homogenizing, pressure 120-400Mpa, circulates homogenizing at least two
Secondary.
Preferably, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is the 0.2-of fresh Intestinum Stichopi japonici quality
0.8%.
Preferably, in described step (4), the enzyme activity of compound protease and neutral protease is than for 2-3:1, and enzyme is total
Consumption is the 0.6-1.0% of fresh Intestinum Stichopi japonici quality.
A kind of preparation method of chelated iron mixed feed, described preparation method is carried out as follows:
The first step: prepare Intestinum Stichopi japonici extract chelated iron,
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, pretreating agent A
For the water-insoluble glucan suspension of mass fraction 8-12%, described pretreating agent B is the reduction of mass fraction 0.5-1.5%
Type glutathion aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is
1:2-3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or
Pretreating agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, under room temperature at stirring
Reason 2-2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is 150-300Da；
(7) chelating: under conditions of 35-45 DEG C, add appropriate ferrous salt in nanofiltration concentrated solution while stirring, delay simultaneously
Slow appropriate amount of acid regulation system pH that adds is 3-4, and the response time is 2～3h, maintenance system pH stable in course of reaction, reaction knot
Cooling down after bundle, filter, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated iron；
Second step, prepares chelated iron mixed feed finished product,
By the defatted rice bran of formula ratio, Semen Maydis powder, sweet potato vine powder, Testa Tritici, soybean cake powder, Intestinum Stichopi japonici extract chelated iron and withered
Grass bacillus cereus mixes in proportion, then through pelletize, dry prepared chelated iron mixed feed.
The invention has the beneficial effects as follows: chelated iron mixed feed rich in nutrition content prepared by the present invention, it is easy to absorb profit
With, absorption rate is high, and impurity and content of beary metal are low, are of high nutritive value, and both can supplement ferrum element, can supplement again multiple
Come from the nutrient of Intestinum Stichopi japonici.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail.
Embodiment 1:
Chelated iron mixed feed, described chelated iron mixed feed is formed by the Raw material processing of following weight portion: defatted rice bran
20 parts, Semen Maydis powder 10 parts, 10 parts of sweet potato vine powder, 10 parts of Testa Tritici, soybean cake powder 15 parts, Intestinum Stichopi japonici extract chelated iron 30 parts, hay
Bacillus cereus 4 parts,
Described chelated iron mixed feed is prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract chelated iron, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pre-
Inorganic agent A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 8%, described pretreatment
Agent B is the reduced glutathion aqueous solution of mass fraction 0.5%, and concrete pretreatment operation is: first use pretreating agent A in often
The lower pretreatment 2h of temperature, solid-liquid ratio is 1:2, then stirs pretreatment 2h under room temperature with pretreating agent B, then stands 45minh, material
Liquor ratio is 1:1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, high pressure homogenize temperature 35-45 DEG C, pressure 120Mpa, circulation homogenizing 3 twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35 DEG C, adjusts pH value to 6.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3min, then cools down
Standing 1h to room temperature, remove supernatant oil layer, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is new fresh sea cucumber
The 0.2% of intestinal quality；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40 DEG C, adjusts pH value 6.5, adds appropriate
Complex food level protease hydrolyzed 2h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 10min, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant, composite flavor albumen
The enzyme activity of enzyme and neutral protease is than for 2:1, and the total consumption of enzyme is the 0.6% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-2000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-200Da；
(7) chelating: under conditions of 35 DEG C, adds moderate amount of sulfuric acid ferrous iron in nanofiltration concentrated solution while stirring, delays simultaneously
Slow appropriate amount of acid regulation system pH that adds is 3, and the response time is 2h, maintenance system pH stable in course of reaction, after reaction terminates
Cooling, filtration, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated iron；
Second step, prepares chelated iron mixed feed finished product,
By the defatted rice bran of formula ratio, Semen Maydis powder, sweet potato vine powder, Testa Tritici, soybean cake powder, Intestinum Stichopi japonici extract chelated iron and withered
Grass bacillus cereus mixes in proportion, then through pelletize, dry prepared chelated iron mixed feed.
Embodiment 2
Chelated iron mixed feed, described chelated iron mixed feed is formed by the Raw material processing of following weight portion: defatted rice bran
30 parts, Semen Maydis powder 20 parts, 15 parts of sweet potato vine powder, 20 parts of Testa Tritici, soybean cake powder 30 parts, Intestinum Stichopi japonici extract chelated iron 40 parts, hay
Bacillus cereus 6 parts, described chelated iron mixed feed prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract chelated iron, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pre-
Inorganic agent A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 12%, described pretreatment
Agent B is the reduced glutathion aqueous solution of mass fraction 1.5%, and concrete pretreatment operation is: first use pretreating agent A in often
The lower pretreatment 1h of temperature, solid-liquid ratio is 1:3, then stirs pretreatment 3h under room temperature with pretreating agent B, then stands 45min, feed liquid
Ratio is 1:1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 60-65 DEG C of high pressure homogenize, pressure 400Mpa, circulation homogenizing twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 45 DEG C, adjusts pH value to 7.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.8% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；Multiple
Closing the enzyme activity of flavor protease and neutral protease ratio for 3:1, the total consumption of enzyme is the 1.0% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
200-300Da；
(7) chelating: under conditions of 45 DEG C, adds appropriate chloritization ferrous iron, simultaneously while stirring in nanofiltration concentrated solution
Being slowly added to appropriate amount of acid regulation system pH is 4, and the response time is 3h, maintenance system pH stable in course of reaction, and reaction terminates
Rear cooling, filtration, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated iron；
Second step, prepares chelated iron mixed feed finished product,
By the defatted rice bran of formula ratio, Semen Maydis powder, sweet potato vine powder, Testa Tritici, soybean cake powder, Intestinum Stichopi japonici extract chelated iron and withered
Grass bacillus cereus mixes in proportion, then through pelletize, dry prepared chelated iron mixed feed.
Embodiment 3:
Chelated iron mixed feed, described chelated iron mixed feed is formed by the Raw material processing of following weight portion: defatted rice bran
25 parts, Semen Maydis powder 15 parts, 12 parts of sweet potato vine powder, 15 parts of Testa Tritici, soybean cake powder 25 parts, Intestinum Stichopi japonici extract chelated iron 35 parts, hay
Bacillus cereus 5 parts, described chelated iron mixed feed prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract chelated iron, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pre-
Inorganic agent A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, described pretreatment
Agent B is the reduced glutathion aqueous solution of mass fraction 1.0%, and concrete pretreatment operation is: by pretreating agent A and pretreatment
Agent B, with volume ratio 1:1 mix homogeneously, is subsequently adding fresh Intestinum Stichopi japonici, and then stir process 2.5h under room temperature stands 1h,
Solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 40-45 DEG C of high pressure homogenize, pressure 180Mpa, circulation homogenizing three times；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 40 DEG C, adjusts pH value to 7.0, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.5% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 45 DEG C, adjusts pH value 6.8, adds appropriate
Complex food level protease hydrolyzed 2.5h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 12min, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant；Composite flavor albumen
The enzyme activity of enzyme and neutral protease is than for 2.5:1, and the total consumption of enzyme is the 0.75% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.3 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
220Da；
(7) chelating: under conditions of 40 DEG C, adds moderate amount of sulfuric acid ferrous iron in nanofiltration concentrated solution while stirring, delays simultaneously
Slow appropriate amount of acid regulation system pH that adds is 3.5, and the response time is 2.5h, maintenance system pH stable in course of reaction, reaction knot
Cooling down after bundle, filter, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated iron；
Second step, prepares chelated iron mixed feed finished product,
By the defatted rice bran of formula ratio, Semen Maydis powder, sweet potato vine powder, Testa Tritici, soybean cake powder, Intestinum Stichopi japonici extract chelated iron and withered
Grass bacillus cereus mixes in proportion, then through pelletize, dry prepared chelated iron mixed feed.
Chelated iron mixed feed prepared by the present invention had both included inorganic iron, include again Organic Iron that Intestinum Stichopi japonici self contains,
Calcium constituent, rich in nutrition content, rich in several amino acids, small-molecular peptides, it is of high nutritive value, it is easy to absorb, absorbs
Rate is high, and impurity content is low, and heavy metal does not detects.
Embodiment described above is the one preferably scheme of the present invention, not makees the present invention any pro forma
Limit, on the premise of without departing from the technical scheme described in claim, also have other variant and remodeling.
Claims (8)
1. chelated iron mixed feed, it is characterised in that: described chelated iron mixed feed is formed by the Raw material processing of following weight portion:
Defatted rice bran 20-30 part, Semen Maydis powder 10～20 parts, sweet potato vine powder 10～15 parts, Testa Tritici 10-20 part, soybean cake powder 15-30 part, sea
Ginseng intestinal extract chelated iron 30-40 part, bacillus subtilis 4-6 part, the preparation method of Intestinum Stichopi japonici extract chelated iron include as
Lower step:
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
(7) chelating: under conditions of 35-45 DEG C, adds appropriate ferrous salt in nanofiltration concentrated solution while stirring, slowly adds simultaneously
Entering appropriate amount of acid regulation system pH is 3-4, and the response time is 2～3h, maintenance system pH stable in course of reaction, after reaction terminates
Cooling, filtration, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated iron.
The preparation method of Intestinum Stichopi japonici Complex Amino Acid Chelated Iron the most according to claim 1, it is characterised in that: described ferrous iron
Salt is ferrous sulfate and/or ferrous chloride.
Chelated iron mixed feed the most according to claim 1, it is characterised in that: described polypeptide feed processed by following raw materials according and
Becoming: defatted rice bran 25 parts, Semen Maydis powder 15 parts, 12 parts of sweet potato vine powder, 15 parts of Testa Tritici, soybean cake powder 25 parts, Intestinum Stichopi japonici extract chelates
Ferrum 35 parts, bacillus subtilis 5 parts.
Chelated iron mixed feed the most according to claim 1, it is characterised in that: in step (1), pretreating agent A is that quality is divided
The water-insoluble glucan suspension of several 10%, described pretreating agent B is that the reduced glutathion of mass fraction 1.0% is water-soluble
Liquid.
Chelated iron mixed feed the most according to claim 1, it is characterised in that: temperature 35-of step (2) mesohigh homogenizing
65 DEG C, pressure 120-400Mpa, circulation homogenizing is at least twice.
Chelated iron mixed feed the most according to claim 1, it is characterised in that: the enzyme work of described food-grade lipase is 2
Ten thousand U/g, enzyme dosage is the 0.4-0.8% of fresh Intestinum Stichopi japonici quality.
Chelated iron mixed feed the most according to claim 1, it is characterised in that: composite flavor albumen in described step (4)
The enzyme activity of enzyme and neutral protease is than for 2-3:1, and the total consumption of enzyme is the 0.6-1.0% of fresh Intestinum Stichopi japonici quality.
8. the preparation method of chelated iron mixed feed described in a claim 1-7 any one, it is characterised in that described preparation
Method is carried out as follows:
The first step: prepare Intestinum Stichopi japonici extract chelated iron,
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
(7) chelating: under conditions of 35-45 DEG C, adds appropriate ferrous salt in nanofiltration concentrated solution while stirring, slowly adds simultaneously
Entering appropriate amount of acid regulation system pH is 3-4, and the response time is 2～3h, maintenance system pH stable in course of reaction, after reaction terminates
Cooling, filtration, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated iron；
Second step, prepares chelated iron mixed feed finished product,
By the defatted rice bran of formula ratio, Semen Maydis powder, sweet potato vine powder, Testa Tritici, soybean cake powder, Intestinum Stichopi japonici extract chelated iron and hay bud
Bacillus mixes in proportion spore, then through pelletize, dry prepared chelated iron mixed feed.
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Application publication date: 20170111 |