CN106305624A - Simple bactrocera cucurbitae temperature stress treatment device and method - Google Patents
Simple bactrocera cucurbitae temperature stress treatment device and method Download PDFInfo
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- CN106305624A CN106305624A CN201610375275.2A CN201610375275A CN106305624A CN 106305624 A CN106305624 A CN 106305624A CN 201610375275 A CN201610375275 A CN 201610375275A CN 106305624 A CN106305624 A CN 106305624A
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- melon trypetid
- temperature stress
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- 238000000034 method Methods 0.000 title claims abstract description 34
- 241001136529 Zeugodacus cucurbitae Species 0.000 title abstract description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 32
- 229920003023 plastic Polymers 0.000 claims abstract description 29
- 239000004033 plastic Substances 0.000 claims abstract description 29
- 239000000523 sample Substances 0.000 claims abstract description 22
- 229910052742 iron Inorganic materials 0.000 claims abstract description 16
- 239000011521 glass Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000219112 Cucumis Species 0.000 claims description 49
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 49
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims description 48
- 238000012545 processing Methods 0.000 claims description 14
- 238000003672 processing method Methods 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 239000002299 complementary DNA Substances 0.000 claims description 5
- 238000003753 real-time PCR Methods 0.000 claims description 4
- 108010085238 Actins Proteins 0.000 claims description 3
- 240000001624 Espostoa lanata Species 0.000 claims description 3
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 239000006059 cover glass Substances 0.000 claims description 3
- 230000009189 diving Effects 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims description 3
- 210000004340 zona pellucida Anatomy 0.000 claims description 3
- 238000010839 reverse transcription Methods 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 102100034051 Heat shock protein HSP 90-alpha Human genes 0.000 claims 1
- 101001016865 Homo sapiens Heat shock protein HSP 90-alpha Proteins 0.000 claims 1
- 238000010222 PCR analysis Methods 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000035882 stress Effects 0.000 description 16
- 239000002917 insecticide Substances 0.000 description 9
- 238000011160 research Methods 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 3
- 101100016370 Danio rerio hsp90a.1 gene Proteins 0.000 description 2
- 101100285708 Dictyostelium discoideum hspD gene Proteins 0.000 description 2
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 2
- 101100071627 Schizosaccharomyces pombe (strain 972 / ATCC 24843) swo1 gene Proteins 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000033870 Citrullus lanatus subsp. vulgaris Species 0.000 description 1
- 235000012840 Citrullus vulgaris Nutrition 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008642 heat stress Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a simple bactrocera cucurbitae temperature stress treatment device which comprises a water bath pot, a glass bottle and an LED submersible lamp, wherein the glass bottle and the LED submersible lamp are arranged in the water bath pot; an iron sheet is mounted at the bottom of the glass bottle; a bottle cover is mounted above the glass bottle; a circular hole is formed in the top of the bottle cover; a plastic pipe and a thermometer probe are arranged in the circular hole; the thermometer probe is connected with a thermometer through a connection wire; a submersible lamp suction disk is mounted on the outer surface of the LED submersible lamp; the upper end of the LED submersible lamp is connected with a power line. The invention further provides a simple bactrocera cucurbitae temperature stress treatment method. Compared with the prior art, the simple bactrocera cucurbitae temperature stress treatment device and the simple bactrocera cucurbitae temperature stress treatment method have the following beneficial effects of flexibility and simplicity, and can be used for stress treatment on both bactrocera cucurbitae and other insects.
Description
Technical field
The present invention is a kind of easy melon trypetid temperature stress processing means and method, belongs to insect resistance technical research
Field, particularly relates to a kind of method utilizing easy device to carry out melon trypetid temperature stress.
Background technology
Melon trypetid (Bactrocera(Zeugodacus)cucurbitae) true Nuscidae
(Tephritidae), it is distributed widely in subtropical zone, is a kind of important invasive insect.Its ovum produces Lu Ke and eggplant
Cause harm in section plant such as Fructus Cucurbitae moschatae, Fructus Melo, Fructus Momordicae charantiae, Citrullus vulgaris, Fructus Persicae and Fructus Lycopersici esculenti etc. and with its transport diffusion.Ambient temperature is to affect insecticide
The key factor of vital movement, the geographical distribution of insecticide and diffusion are the most affected by the ambient temperature.Temperature stress tolerates
Property research prediction insect occurrence and distribution district, set up bioclimatology model, simulation population dynamic, quarantining treatment, introduction natural enemy, change
Enter the aspect such as pest control strategies and its geographical spread ability of assessment and there is important directive significance.Research shows, insecticide is to height
In the toleration consubstantiality of temperature or low temperature, the expression of heatshock protein family portion gene has close relationship, and therefore researcher passes through
Research short time under specific high temperature or low temperature coerces insecticide, then studies its internal that heat shock protein gene and there occurs
Adjust or lower and express, thus further appreciate that the temperature tolerance of insecticide.
The instrument that insecticide carries out temperature stress is mainly growth cabinet, but growth cabinet price costly and accounts for
Relatively big with space, for short time temperature stress processes insecticide, use and inconvenient.Therefore, a kind of letter is worked out
Insecticide temperature stress processing method is necessary for being engaged in research in this respect the most easily.
Summary of the invention
The deficiency existed for prior art, it is an object of the present invention to provide a kind of easy melon trypetid temperature stress and processes dress
Putting and method, to solve the problem proposed in above-mentioned background technology, the present invention is easy to use, it is simple to operation, good stability, reliably
Property high.
To achieve these goals, the present invention is to realize by the following technical solutions: a kind of easy melon trypetid temperature
Degree Stress treatment device, is placed on water including water-bath, vial and LED submarine light, described vial and LED submarine light
In bath, the bottom of described vial is provided with iron plate, described vial be arranged above bottle cap, the top of described bottle cap is opened
Having circular hole, be placed with plastic tube and thermometer probe in described circular hole, described thermometer probe connects temperature by connecting line
Meter, the outer surface of described LED submarine light is provided with submarine light sucker, and the upper end of described LED submarine light connects power line.
Further, the bottom of described water-bath is placed with Magnet.
Further, being opened in the circular hole above bottle cap and be provided with three, a diameter of 6mm of described circular hole, plastic tube is provided with
Two, two plastic tubes are placed in two circular holes, are placed with thermometer probe in remaining a circular hole.
Further, the external diameter of described plastic tube, internal diameter and long respectively 6mm, 4mm and 50mm, the bottom of plastic tube
Connecting and have gauze, the mesh number of described gauze is for for 40 mesh.
Further, described plastic tube and thermometer probe are to utilize PUR to carry out cohering being enclosed in circular hole.
Further, described LED diving connects external circuit by power line.
Further, described iron plate carries out cohering the lower section at vial by PUR, and described iron plate is rounded, described
A diameter of 2.5cm of iron plate.
Further, described water-bath is a kind of cold and hot type water-bath.
Further, described vial is Clear glass bottles and jars, and the volume of vial is 100ml.
A kind of easy melon trypetid temperature stress processing method, comprises the following steps:
Step one, open the circular hole of three diameter 6mm diameters in the Bottle cap top of 100ml zona pellucida cover glass bottle；
Step 2, the yarn sealing up 40 mesh bottom two external diameters, internal diameter and the long plastic tube being respectively 6mm, 4mm and 50mm
Net, then coheres plastic tube PUR in two circular holes on bottle cap, with PUR, thermometer probe is cohered surplus
In a remaining circular hole and go deep into 50mm in bottle cap；
Step 3, paste the round iron sheet of one piece of diameter 2.5cm at vial outer bottom；
Step 4, pending melon trypetid is introduced in vial, cover bottle cap, wherein a plastic tube puts into a cotton
Ball, and drip the hydromel of 5%-10% as food；
Step 5, water-bath inwall place LED submarine light, as light source；
Step 6, water-bath is modulated temperature required, use Magnet vial is fixed in water-bath, pour water, water logging into
Bottle cap, according to the illumination arranged and the time of process, complete rear taking-up examination worm；
Step 7, employing fluorescent quantitative PCR technique are internal after analyzing melon trypetid heat stresshsp90The relative expression quantity of gene.
Further, in step 4, concrete step is, the pending emergence melon trypetid adult male and female each 4 of 3-5 days
Head is incorporated in vial, covers bottle cap.
Further, in step 5, the distance of vial and LED submarine light is between 5-10cm.
Further, in step 6, the temperature 40 DEG C that water-bath sets, controlled temperature is 26 DEG C, treats temperature in vial
Degree reach temperature required after start timing, the process time is respectively 1h, 3h and 5h, process terminate after take out melon trypetid, room temperature is extensive
Multiple 1h, carries out subsequent step.
Further, in step 7, the melon trypetid after room temperature recovers 1h uses Trizol reagent to extract its total serum IgE, instead
Transcribe synthesis cDNA, according to melon trypetid hsp90 and actin gene order, process the cDNA of sample as template with each, fluorescence
After quantitative PCR analysis temperature stresshsp90The relative expression quantity of gene.
Beneficial effects of the present invention: a kind of easy melon trypetid temperature stress processing means of the present invention and method, flexibly
Simply, not only may be used for melon trypetid, it is also possible to for the Stress treatment of other insecticide.
Accompanying drawing explanation
By the detailed description non-limiting example made with reference to the following drawings of reading, the further feature of the present invention,
Purpose and advantage will become more apparent upon:
Fig. 1 is a kind of easy melon trypetid temperature stress processing means of the present invention and the LED submarine light structural representation of method；
Fig. 2 is a kind of easy melon trypetid temperature stress processing means of the present invention and the structural blast figure of method；
Fig. 3 is that 40 DEG C of high temperature coerce melon trypetid adult under 1h, 3h and 5h respectivelyhsp90Gene relative expression quantity result figure.
26 DEG C process insects as comparison, each process three repetitions, figure center pillar subrepresentation relative expression quantity ± SE, on post difference words
Matrix shows at the upper significant difference of 5% level (Tkey ' s test).Hsp90Gene relative quantification expression uses 2-△△tAnalyze
Method, wherein A is female adult worm, and B is male imago.
In figure: 1-water-bath, 2-vial, 3-LED submarine light, 21-iron plate, 22-Magnet, 23-bottle cap, 24-circular hole,
25-plastic tube, 26-thermometer probe, 27-connecting line, 28-thermometer, 31-power line, 32-submarine light sucker.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below in conjunction with
Detailed description of the invention, is expanded on further the present invention.
Referring to Fig. 1, Fig. 2, the present invention provides a kind of technical scheme: a kind of easy melon trypetid temperature stress processes dress
Putting, including water-bath 1, vial 2 and LED submarine light 3, vial 2 and LED submarine light 3 is placed in water-bath 1, glass
The bottom of glass bottle 2 is provided with iron plate 21, vial 2 be arranged above bottle cap 23, the top of bottle cap 23 has circular hole 24, circular hole
Being placed with plastic tube 25 and thermometer probe 26 in 24, thermometer probe 26 connects thermometer 28 by connecting line 27, and LED dives
The outer surface of water lamp 3 is provided with submarine light sucker 32, and the upper end of LED submarine light 3 connects power line 31.
The bottom of water-bath 1 is placed with Magnet 22, and iron plate 21 carries out cohering the lower section at vial 2, ferrum by PUR
Sheet 21 is rounded, a diameter of 2.5cm of iron plate 21, and water-bath 1 is a kind of cold and hot type water-bath.
It is opened in the circular hole 24 above bottle cap 23 and is provided with three, a diameter of 6mm of circular hole 24, plastic tube 25 is provided with two,
Two plastic tubes 25 are placed in two circular holes 24, are placed with thermometer probe 26 in remaining a circular hole 24.
The external diameter of plastic tube 25, internal diameter and long respectively 6mm, 4mm and 50mm, the bottom of plastic tube 25 connects yarn
Net, the mesh number of gauze is for being to utilize PUR to carry out cohering and be enclosed in circular hole 24 for 40 mesh, plastic tube 25 and thermometer probe 26
In.
LED diving connects external circuit by power line 31, and vial 2 is Clear glass bottles and jars 2, and the volume of vial 2 is
100ml。
As one embodiment of the present of invention, it is provided that a kind of easy melon trypetid temperature stress processing method, including following
Step:
Step one, open the circular hole 24 of three diameter 6mm diameters at bottle cap 23 top of 100ml zona pellucida cover glass bottle 2；
Step 2, bottom two external diameters, internal diameter and the long plastic tube 25 being respectively 6mm, 4mm and 50mm, seal up 40 purposes
Gauze, then coheres in two circular holes 24 on bottle cap 23 by plastic tube 25 PUR, with PUR thermometer probe
26 cohere in a remaining circular hole 24 and go deep into 50mm in bottle cap 23；
Step 3, paste the round iron sheet of one piece of diameter 2.5cm at vial 2 outer bottom；
Step 4, pending melon trypetid is introduced in vial 2, cover bottle cap 23, wherein a plastic tube 25 is put into
One cotton balls, and drip the hydromel of 5%-10% as food；
Step 5, water-bath 1 inwall place LED submarine light 3, as light source；
Step 6, water-bath 1 is modulated temperature required, use Magnet that vial 2 is fixed in water-bath 1, pour water, water logging into
Cross bottle cap 23, according to the illumination arranged and the time of process, complete rear taking-up examination worm；
Step 7, employing fluorescent quantitative PCR technique are internal after analyzing melon trypetid heat stresshsp90The relative expression of gene
Amount.
In step 4, concrete step is, pending emergence each 4 of the melon trypetid adult male and female of 3-5 days are incorporated into
In vial 2, cover bottle cap 23.
In step 5, the distance of vial 2 and LED submarine light 3 is between 5-10cm.
In step 6, the temperature 40 DEG C that water-bath 1 sets, controlled temperature is 26 DEG C, and in treating vial 2, temperature reaches institute
Starting timing after needing temperature, the process time is respectively 1h, 3h and 5h, and process takes out melon trypetid after terminating, and room temperature recovers 1h, carries out
Subsequent step.
In step 7, the melon trypetid after room temperature recovers 1h uses Trizol reagent to extract its total serum IgE, and reverse transcription synthesizes
CDNA, according to melon trypetidhsp90With actin gene order, use the synthesis of primer5.0 software design for fluorescent quantitation
The primer analyzed: hsp90-F/R:ACTTCCACTATGGGCTAC/ CCACCGCTTTGAGTATCT, actin-F/R:
ATGAATTGCCTGATGGTC/ GAATGAGGGTTGGAAGAGT;With each process sample cDNA as template, fluorescent quantitation
After pcr analysis temperature stresshsp90The relative expression quantity of gene.
After melon trypetid heat stresshsp90Gene expression results is shown in Fig. 3.Result shows at 40 DEG C of difference heat stress melons real
After fly 1h, 3h and 5h, in male and female polypidehsp90Gene relative expression quantity more all there occurs significantly compared with 26 DEG C of comparisons
Property up-regulated expression；What expression was the highest be 40 DEG C coerces 1h process, in female polypidehsp90Expression has raised 11.25
Times, in male worm bodyhsp90Expression has raised 7.64 times；When coercing 3h process for 40 DEG C, in female polypidehsp90Express
Amount has raised 5.42 times, and in male worm body, hsp90 expression has raised 4.98 times；When coercing 5h process for 40 DEG C, in female polypidehsp90Expression has raised 4.21 times, in male worm bodyhsp90Expression has raised 4.96 times.Sample result shows,
In melon trypetid bodyhsp90The up-regulated expression of gene may be relevant with its thermostability, and its up-regulated expression is not to coerce with heat
The time lengthening compeled and raise, increase the most in time and decline.
The ultimate principle of the present invention and principal character and advantages of the present invention are more than shown and described, for this area skill
For art personnel, it is clear that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and without departing substantially from the present invention spirit or
In the case of basic feature, it is possible to realize the present invention in other specific forms.Therefore, no matter from the point of view of which point, all should be by
Embodiment regards exemplary as, and is nonrestrictive, the scope of the present invention by claims rather than on state
Bright restriction, it is intended that include all changes fallen in the implication of equivalency and scope of claim in the present invention
In.Should not be considered as limiting involved claim by any reference in claim.
Although moreover, it will be appreciated that this specification is been described by according to embodiment, but the most each embodiment only wraps
Containing an independent technical scheme, this narrating mode of description is only that for clarity sake those skilled in the art should
Description can also be formed those skilled in the art through appropriately combined as an entirety, the technical scheme in each embodiment
May be appreciated other embodiments.
Claims (14)
1. an easy melon trypetid temperature stress processing means, including water-bath, vial and LED submarine light, its feature
Being: described vial and LED submarine light are placed in water-bath, the bottom of described vial is provided with iron plate, described glass
Glass bottle be arranged above bottle cap, the top of described bottle cap has circular hole, is placed with plastic tube and thermometer in described circular hole
Probe, described thermometer probe connects thermometer by connecting line, and the outer surface of described LED submarine light is provided with submarine light and inhales
Dish, the upper end of described LED submarine light connects power line.
One the most according to claim 1 easy melon trypetid temperature stress processing means, it is characterised in that: described water-bath
The bottom of pot is placed with Magnet.
One the most according to claim 1 easy melon trypetid temperature stress processing means, it is characterised in that: it is opened in bottle
The circular hole of the side of covering is provided with three, a diameter of 6mm of described circular hole, and plastic tube is provided with two, and two plastic tubes are placed on two
In circular hole, in remaining a circular hole, it is placed with thermometer probe.
One the most according to claim 3 easy melon trypetid temperature stress processing means, it is characterised in that: described plastics
The external diameter of pipe, internal diameter and long respectively 6mm, 4mm and 50mm, the bottom of plastic tube connects gauze, the mesh number of described gauze
For for 40 mesh.
One the most according to claim 3 easy melon trypetid temperature stress processing means, it is characterised in that: described plastics
Pipe and thermometer probe are to utilize PUR to carry out cohering being enclosed in circular hole.
One the most according to claim 1 easy melon trypetid temperature stress processing means, it is characterised in that: described LED
Diving connects external circuit by power line.
One the most according to claim 1 easy melon trypetid temperature stress processing means, it is characterised in that: described iron plate
Carrying out cohering the lower section at vial by PUR, described iron plate is rounded, a diameter of 2.5cm of described iron plate.
One the most according to claim 1 easy melon trypetid temperature stress processing means, it is characterised in that: described water-bath
Pot is a kind of cold and hot type water-bath.
One the most according to claim 1 easy melon trypetid temperature stress processing means, it is characterised in that: described glass
Bottle is Clear glass bottles and jars, and the volume of vial is 100ml.
10. an easy melon trypetid temperature stress processing method, it is characterised in that: comprise the following steps:
Step one, open the circular hole of three diameter 6mm diameters in the Bottle cap top of 100ml zona pellucida cover glass bottle；
Step 2, the yarn sealing up 40 mesh bottom two external diameters, internal diameter and the long plastic tube being respectively 6mm, 4mm and 50mm
Net, then coheres plastic tube PUR in two circular holes on bottle cap, with PUR, thermometer probe is cohered surplus
In a remaining circular hole and go deep into 50mm in bottle cap；
Step 3, paste the round iron sheet of one piece of diameter 2.5cm at vial outer bottom；
Step 4, pending melon trypetid is introduced in vial, cover bottle cap, wherein a plastic tube puts into a cotton
Ball, and drip the hydromel of 5%-10% as food；
Step 5, water-bath inwall place LED submarine light, as light source；
Step 6, water-bath is modulated temperature required, use Magnet vial is fixed in water-bath, pour water, water logging into
Bottle cap, according to the illumination arranged and the time of process, complete rear taking-up examination worm；
Step 7, employing fluorescent quantitative PCR technique are internal after analyzing melon trypetid heat stresshsp90The relative expression quantity of gene.
11. one according to claim 10 easy melon trypetid temperature stress processing method, it is characterised in that: in step
In four, concrete step is, pending emergence each 4 of the melon trypetid adult male and female of 3-5 days is incorporated in vial, covers bottle
Lid.
12. one according to claim 10 easy melon trypetid temperature stress processing method, it is characterised in that: in step
In five, the distance of vial and LED submarine light is between 5-10cm.
13. one according to claim 10 easy melon trypetid temperature stress processing method, it is characterised in that: in step
In six, water-bath set temperature 40 DEG C, controlled temperature is 26 DEG C, until temperature in vial reach temperature required after start meter
Time, the process time is respectively 1h, 3h and 5h, and process takes out melon trypetid after terminating, and room temperature recovers 1h, carries out subsequent step.
14. one according to claim 10 easy melon trypetid temperature stress processing method, it is characterised in that: in step
In seven, the melon trypetid after room temperature recovers 1h uses Trizol reagent to extract its total serum IgE, and reverse transcription synthesis cDNA, according to melon trypetid
Hsp90 and actin gene order, with each process sample cDNA as template, after quantitative fluorescent PCR analysis temperature is coercedhsp90The relative expression quantity of gene.
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| CN201610375275.2A CN106305624B (en) | 2016-05-31 | 2016-05-31 | A kind of easy melonfly temperature stress processing unit and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109006744A (en) * | 2018-06-20 | 2018-12-18 | 广东省农业科学院植物保护研究所 | Citrus fruit fly irradiates processing system and the processing method of preventing epidemic of not sprouting wings |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109006744A (en) * | 2018-06-20 | 2018-12-18 | 广东省农业科学院植物保护研究所 | Citrus fruit fly irradiates processing system and the processing method of preventing epidemic of not sprouting wings |
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