CN109486679A - A kind of evaluation and test of extracorporeal blood vessel bracket micro-fluidic chip and application - Google Patents
A kind of evaluation and test of extracorporeal blood vessel bracket micro-fluidic chip and application Download PDFInfo
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Abstract
The invention belongs to micro fluidic chip technical fields, and in particular to a kind of evaluation and test of extracorporeal blood vessel bracket micro-fluidic chip and application.The micro-fluidic chip is mainly made of sample-adding valve 1, Circuluting puls Micropump 6, cell culture insert 4, and the plate-like channel 3 of the micro-fluidic chip is closed with amberplex.The micro-fluidic chip can reduce the consumption of the time cost and cost of material of evaluation and test experiment demand, it can be ensured that the identity tested under many condition, it is ensured that the comparativity of experimental result；The amberplex of use, guarantee cell culture area cation concn it is constant while effectively shield the interference of other ions, realize tightness during the experiment it is convenient and efficient so that experiment reaction is integrated with result detection.
Description
Technical field
The invention belongs to micro fluidic chip technical fields, and in particular to micro-fluidic chip is used in a kind of evaluation and test of extracorporeal blood vessel bracket
And application.
Background technique
Cardiovascular disease illness rate rises every year, the case where according to China human mortality aging situation and population growth rate, in advance
The illness rate of meter China's cardiovascular disease can increase to 50% in the year two thousand thirty, and seriousness can not be ignored with generality.And it controls at present
Treating the most important therapeutic modality of cardiovascular disease is exactly intervenient cure, and wherein the implantation of intravascular stent is interventional therapy again
In most common means.The new intravascular stent for being more suitable for environment in human body complexity of the research and development angiocarpy increasingly serious to alleviation
Disease incidence has great significance.
For the correlative study of intravascular stent,Evaluate and test bracket after implantable intravascular between blood flow and vascular tissue Reciprocal effects are unusual the key links, this is related to the incidence and the late Stage of Postoperative heart of clinical adverse events after stenter to implant The recurrence rate of vascular diseases guarantees that bracket can play therapeutic effect as expected and will not generate other fatal later period pairs and makees With.In addition to this, studying power-biological mechanism therein is also the important thrust for verifying cardiovascular disease and occurring and developing, can
To carry out special sex modification transformation, building optimization intervention plan to bracket itself or bracket coating by related mechanism.
Currently, related evaluation and test research experiment is generally confined in animal model test.And zoopery limiting factor is quite
It is more, such as to the control of animal physiological state and interventional conditions,The raising of experimental animal and animal model building expense etc., The disadvantages of there are also the preparatory period is long, preparation routine is complicated.However the evaluation and test of bracket experiment is again essential.
Therefore, in order to enable researchers to quick and convenient completion intravascular stent evaluation and test experiment, also for can add
The flow of research of fast intravascular stent, solves the problems, such as the treatment of closed coronary artery disease, using microflow control technique, constructs body
The outer micro-fluidic torus device for carrying out related evaluation and test experiment is very necessary.
Summary of the invention
In view of this, one of the objects of the present invention is to provide a kind of extracorporeal blood vessel bracket evaluation and test micro-fluidic chip, with
Reduce the consumption of the time cost and cost of material of experiment demand, it is ensured that the identity tested under many condition, it is ensured that experimental result
Comparativity.
To achieve the above object, the present invention uses following scheme:
A kind of extracorporeal blood vessel bracket evaluation and test micro-fluidic chip, mainly by sample-adding valve 1, Circuluting puls Micropump 6, cell culture
Pond 4 forms.
Further, the chip is rounded or rectangle, and position is machined with 2 cylindrical sample-adding valves 1 side by side in its center；
Each cylindrical sample-adding valve 1 is machined with 2 opposite openings on same diameter of section line, each opening be separately connected 1 into
Sample pipe 2；Each sample feeding pipe 2 respectively leads to a respective cell culture insert 4, totally 4 cell culture inserts 4, trains with cell
The plate-like channel 3 for supporting bottom processing in pond 4 communicates.
It is machined between 2 sample ports, 8,2 sample ports 8 between 2 round sample-adding valves 1 side by side in the middle part of the chip
It is machined with sample channel 9 to be interconnected, is machined with shut-off valve 11 on the sample channel 9 of connection；2 sample ports 8 are also each
2 sample channels 9 are machined with to be connected to 2 cell culture inserts 4 respectively.
The sample feeding pipe 2 is respectively machined with 1 one-way circulation pipe 10, connection sample-adding 1 both ends of valve on 21 sides of sample-adding valve
Sample feeding pipe 2；Shut-off valve 11 is all machined on every one-way circulation pipe 10.
The cylindrical interior of the sample-adding valve 1 is valve body 16, and a upper opening, end size are machined with inside valve body 16
The valve internal channel 14 cooperated with sample feeding pipe 2；16 top of valve body is machined with valve button 15.
Further, 4 cell culture inserts 4 are evenly distributed at four angles of rectangular dies, or round chip is symmetrical
Four sides at；Processing is equipped with 1 Circuluting puls Micropump 6 between a pair of of cell culture insert 4, and Circuluting puls Micropump 6 is ganged up
Among 2 circulation pipes 5,2 circulation pipes 5 respectively with the plate-like channel 3 in the cell culture insert 4 of 6 both sides of Circuluting puls Micropump
It is connected.
Further, the circulation pipe 5 and sample feeding pipe 2 be at the T-type interface 17 on 4 side of cell culture insert with plate-like channel 3
It is connected to jointly.
Further, the plate-like channel 3 is spiraled at the center of cell culture insert 4 along 4 bottom surface of cell culture insert, with processing
The bracket experiment tube 7 at center is fixed on to communicate；The bracket experiment tube 7 is an elastic material, is higher by cell culture insert 4
Hollow pipe is equipped with sampling chock plug 13 at top end opening；It samples and pops one's head in inside chock plug 13 equipped with pressure sensing.
The sampling chock plug 13 can penetrate sampling syringe needle sampling, and the diameter dimension of the bracket experiment tube 7 has different rule
Lattice, the intravascular stents 12 of different tube diameters can be in fitting sles.Pressure sensing probe inside the sampling chock plug 13 can will follow
The pressure signal and interval that ring pulsation Micropump 6 generates in bracket experiment tube 7, are passed on display.
Further, all sample feeding pipes 2 of the chip, plate-like channel 3, circulation pipe 5, sample channel 9, one-way circulation pipe
10, bracket experiment tube 7 is all sealing pipeline, can guarantee the pressure that all pipelines can be generated by Circuluting puls Micropump 6 in this way.
The second object of the present invention is that the plate-like for providing a kind of extracorporeal blood vessel bracket evaluation and test micro-fluidic chip is logical
Road 3.
To achieve the above object, the present invention uses following scheme:
The plate-like channel 3, is closed with amberplex, guarantees solion and cell culture insert 4 in plate-like channel 3
Solution can not mix；There is pipeline partition wall 19 among the plate-like channel 3, is divided into 2 from T-type interface 17 by plate-like channel 3
A plate-like double small pipe 18 ensure that the reagent in plate-like channel 3 can recycle under connected state in circulation pipe 5 and sample feeding pipe 2
Reagent circulation.
The Ion reagent in plate-like channel 3 can be guaranteed because concentration difference penetrates into always cell using amberplex closing
Culture pond 4, and keep 4 iso-ionia of cell culture insert.On the one hand, can stablize in chip, especially in plate-like channel
Ambient condition is stablized, and pH value does not have great fluctuation process；On the other hand, in pathology environmental simulation, also play the role of it is stable, such as
Simulate the raised interior state of calcium ion level caused by inflammation.
The plate-like channel 3 can transmit the pressure that the Circuluting puls Micropump 6 received generates, and can add some energy
Enough reagents by amberplex.
The third object of the present invention is to provide a kind of application of extracorporeal blood vessel bracket evaluation and test micro-fluidic chip have
Body is the application in intravascular stent is evaluated and tested in vitro.
The extracorporeal blood vessel bracket evaluation and test provided by the invention is the knot of the circulatory system and cell culture with micro-fluidic chip
It closes, suitable for the evaluation and test experimental work of intravascular stent, research work.
In certain embodiments, the experiment thought progress that the evaluation and test experiment of extracorporeal blood vessel bracket follows control unique variable is carried out
Design.
When cell is evaluated and tested as variable: common to study cell class when studying blood vessel and intravascular stent relevant issues
Not.Including but not limited to: Human umbilical vein endothelial cells (HUVEC), human umbilical artery smooth muscle cells (HUASMC), rat chest are dynamic greatly
Smooth muscle cells (A7r5).
When culture environment is evaluated and tested as variable: culture medium difference such as RPMI-1640 culture medium, DMEM culture medium (includes
High sugar, middle sugar and low sugar formula)；Chip can by the control of valve realize compartment separation, repopulating cell it is identical, install additional branch
Under the premise of the specification of frame is identical, the additional cytosis factor is added in different compartments, as inflammatory factor and cell are grown
Factor etc..In theory, it as long as culture medium can be dissolved in, or can be dispersed in culture medium, cell can be produced
The substance of raw certain effect all can serve as to construct the tool of different culture environments.
When intravascular stent is evaluated and tested as variable: the difference of bracket can not only be embodied in its specification and not be same as above, can also be with
It is embodied in material itself, surfacial pattern, on surface covering.Even the bracket of same size, rack surface coating mounted
Ingredient or the mode for carrying coating are different, also can be used as unique variable and compare test.
The fourth object of the present invention is to provide a kind of application of amberplex that the plate-like channel 3 uses, specifically
For application of the amberplex in intravascular stent is evaluated and tested in vitro.
The amberplex cationic channel is combined with cation-exchange membrane, is continuously passed through solion,
Guarantee cell culture area cation concn it is constant while, amberplex effectively shields the interference of other ions,
Chip of the present invention is a closed circulatory system, and the liquid in chip is reflowable.
The beneficial effects of the present invention are:
1) a kind of extracorporeal blood vessel bracket evaluation and test micro-fluidic chip of the invention can reduce evaluation and test experiment demand when
Between cost and cost of material consumption, overcome raising and animal model building expense of experimental animal etc., there are also the preparatory periods
Long, the disadvantages of preparation routine is complicated；
2) a kind of extracorporeal blood vessel bracket evaluation and test of the invention can ensure that the identity tested under many condition with micro-fluidic chip,
Ensure the comparativity of experimental result；
3) amberplex that a kind of extracorporeal blood vessel bracket evaluation and test of the invention is used with micro-fluidic chip, is guaranteeing thin
Born of the same parents cultivation region cation concn it is constant while effectively shield the interference of other ions, realize during the experiment
Tightness, so that experiment reaction is integrated with result detection, it is convenient and efficient.
Detailed description of the invention
Fig. 1 is the schematic view of the front view of rectangular dies.
Fig. 2 is the schematic view of the front view of round chip.
Fig. 3 is the partial schematic sectional view of a cell culture insert.
Fig. 4 is a kind of schematic view of the front view being loaded under valve opening state.
Fig. 5 is a kind of overlooking structure diagram being loaded under valve opening state.
Fig. 6 is a kind of schematic view of the front view being loaded under valve closing state.
Fig. 7 is a kind of overlooking structure diagram being loaded under valve closing state.
Fig. 8 is a kind of partial schematic diagram of the T-type interface of cell culture insert.
Fig. 9 is the SEM scan image on different coating surface.
Figure 10 is each group coating bracket smooth surface muscle cell multiplication experimental result.
In figure: 1. sample-adding valves；2. sample feeding pipe；3. plate-like channel；4. cell culture insert；5. circulation pipe；6. Circuluting puls are micro-
Pump；7. bracket experiment tube；8. sample port；9. sample channel；10. one-way circulation pipe；11. shut-off valve；12. intravascular stent；13. taking
Sample chock plug；14. valve internal channel；15. valve button；16. valve body；17.T type interface；18. plate-like double small pipe；19. pipeline partition wall.
Specific embodiment
Illustrated embodiment is in order to which preferably the present invention will be described, but is not that the contents of the present invention are limited only to institute
For embodiment.So those skilled in the art according to foregoing invention content to embodiment carry out it is nonessential improvement and
Adjustment, still falls within protection scope of the present invention.
Micro-fluidic chip is used in a kind of evaluation and test of the extracorporeal blood vessel bracket of embodiment 1
The micro-fluidic rectangular dies of a kind of extracorporeal blood vessel bracket evaluation and test, as shown in Figure 1, mainly by sample-adding valve 1, Circuluting puls
Micropump 6, cell culture insert 4 form, and position is machined with 2 cylindrical sample-adding valves 1 side by side, each cylindrical sample-adding in its center
Valve 1 is machined with 2 opposite openings on same diameter of section line, and each opening is separately connected 1 sample feeding pipe 2；It is described each
Sample feeding pipe 2 respectively leads to a respective cell culture insert 4, and bottom processes in totally 4 cell culture inserts 4, with cell culture insert 4
Plate-like channel 3 communicate.
4 cell culture inserts 4 are evenly distributed at symmetrical four sides of round chip；In a pair of of cell culture insert 4
Between processing 1 Circuluting puls Micropump 6 is installed, Circuluting puls Micropump 6 is ganged up among 2 circulation pipes 5, and 2 circulation pipes 5 divide
It is not connected with the plate-like channel 3 in the cell culture insert 4 of 6 both sides of Circuluting puls Micropump；The circulation pipe 5 and sample feeding pipe 2 are
It is connected to jointly at the T-type interface 17 on 4 side of cell culture insert with plate-like channel 3；There is pipeline partition wall 19 among plate-like channel 3,
It is divided into 2 plate-like double small pipes 18 from T-type interface 17 by plate-like channel 3；Plate-like channel 3 is spiraled into carefully along 4 bottom surface of cell culture insert
At the center of born of the same parents' culture pond 4, the bracket experiment tube 7 being fixed at center with processing is communicated；The bracket experiment tube 7 is an elasticity
Material, be higher by the hollow pipe of cell culture insert 4, be equipped at top end opening sampling chock plug 13；It is filled inside sampling chock plug 13
There is pressure sensing probe.
It is machined between 2 sample ports, 8,2 sample ports 8 between 2 round sample-adding valves 1 side by side in the middle part of the chip
It is machined with sample channel 9 to be interconnected, is machined with shut-off valve 11 on the sample channel 9 of connection；2 sample ports 8 are also each
2 sample channels 9 are machined with to be connected to 2 cell culture inserts 4 respectively.
The sample feeding pipe 2 is respectively machined with 1 one-way circulation pipe 10, connection sample-adding 1 both ends of valve on 21 sides of sample-adding valve
Sample feeding pipe 2；Shut-off valve 11 is all machined on every one-way circulation pipe 10.
All sample feeding pipes 2, plate-like channel 3, circulation pipe 5, sample channel 9, one-way circulation pipe 10, the bracket of the chip are real
Testing pipe 7 is all sealing pipeline；It is closed with amberplex in the plate-like channel 3；The cylindrical interior of the sample-adding valve 1 is valve body
16, a upper opening, the valve internal channel 14 that end size and sample feeding pipe 2 cooperate are machined with inside valve body 16；16 top of valve body
It is machined with valve button 15.
The sampling chock plug 13 can penetrate sampling syringe needle sampling；Sampling can equipped with pressure sensing probe inside chock plug 13
Fluid pressure in bracket experiment tube 7 is output to display；The diameter dimension of the bracket experiment tube 7 has different size, no
Intravascular stent 12 with caliber can be in fitting sle.
Micro-fluidic chip is used in a kind of evaluation and test of the extracorporeal blood vessel bracket of embodiment 2
The micro-fluidic round chip of a kind of extracorporeal blood vessel bracket evaluation and test, as shown in Fig. 2, mainly by sample-adding valve 1, Circuluting puls
Micropump 6, cell culture insert 4 form, and position is machined with 2 cylindrical sample-adding valves 1 side by side, each cylindrical sample-adding in its center
Valve 1 is machined with 2 opposite openings on same diameter of section line, and each opening is separately connected 1 sample feeding pipe 2；It is described each
Sample feeding pipe 2 respectively leads to a respective cell culture insert 4, and bottom processes in totally 4 cell culture inserts 4, with cell culture insert 4
Plate-like channel 3 communicate.
4 cell culture inserts 4 are evenly distributed at four angles of rectangular dies, between a pair of of cell culture insert 4
Processing 1 Circuluting puls Micropump 6 is installed, Circuluting puls Micropump 6 is ganged up among 2 circulation pipes 5,2 circulation pipes 5 respectively with
Plate-like channel 3 in the cell culture insert 4 of 6 both sides of Circuluting puls Micropump is connected；The circulation pipe 5 and sample feeding pipe 2 are thin
It is connected to jointly at the T-type interface 17 on 4 side of born of the same parents' culture pond with plate-like channel 3；There is pipeline partition wall 19 among plate-like channel 3, from T-type
Plate-like channel 3 is divided into 2 plate-like double small pipes 18 by interface 17；Plate-like channel 3 is spiraled into cell culture along 4 bottom surface of cell culture insert
At the center in pond 4, the bracket experiment tube 7 being fixed at center with processing is communicated；The bracket experiment tube 7 is an elastic material
, hollow pipe that be higher by cell culture insert 4, sampling chock plug 13 is installed at top end opening；It samples inside chock plug 13 equipped with pressure
Force sensing probe.
It is machined between 2 sample ports, 8,2 sample ports 8 between 2 round sample-adding valves 1 side by side in the middle part of the chip
It is machined with sample channel 9 to be interconnected, is machined with shut-off valve 11 on the sample channel 9 of connection；2 sample ports 8 are also each
2 sample channels 9 are machined with to be connected to 2 cell culture inserts 4 respectively.
The sample feeding pipe 2 is respectively machined with 1 one-way circulation pipe 10, connection sample-adding 1 both ends of valve on 21 sides of sample-adding valve
Sample feeding pipe 2；Shut-off valve 11 is all machined on every one-way circulation pipe 10.
All sample feeding pipes 2, plate-like channel 3, circulation pipe 5, sample channel 9, one-way circulation pipe 10, the bracket of the chip are real
Testing pipe 7 is all sealing pipeline；It is closed with amberplex in the plate-like channel 3；The cylindrical interior of the sample-adding valve 1 is valve body
16, a upper opening, the valve internal channel 14 that end size and sample feeding pipe 2 cooperate are machined with inside valve body 16；16 top of valve body
It is machined with valve button 15.
The sampling chock plug 13 can penetrate sampling syringe needle sampling；Sampling can equipped with pressure sensing probe inside chock plug 13
Fluid pressure in bracket experiment tube 7 is output to display；The diameter dimension of the bracket experiment tube 7 has different size, no
Intravascular stent 12 with caliber can be in fitting sle.
The evaluation and test experiment of extracorporeal blood vessel bracket is carried out based on micro-fluidic chip under the different cytosiies of embodiment 3
Using micro-fluidic chip described in embodiment 1, all shut-off valves 11 and sample-adding valve 1 are closed.By identical blood vessel branch
12 sets of frame on matched bracket experiment tube 7.Identical culture medium is added from sample port 8 and flows into cell culture insert 4.It will be different
Living cells (such as Human umbilical vein endothelial cells, human umbilical artery smooth muscle cells and rat chest aortic smooth muscle cells) suspension connects
Kind is on intravascular stent 12, after cell is adherent, opens shut-off valve 11, the shut-off valve among two of them sample port 8 needs to protect
Closing is held, Circuluting puls Micropump 6 is adjusted, related experiment can be started.During the experiment, sample-adding valve 1 is in close state, root
According to the state of cell in cell culture insert 4, (or first plus non-ion reagent, then refinement cell culture fluid is added by sample port 8
Born of the same parents' culture solution), it can be sampled analysis by sampling chock plug 13, be degraded by experiment front and back support or deformation extent, rack surface
Experimental data, evaluation experimental result are collected in the variation of ingredient in the adherency of cell and extent of growth, culture solution.
Application of 4 amberplex of embodiment in the intravascular stent under the effect of different culture environments is evaluated and tested in vitro
The micro-fluidic chip using amberplex close plate-like channel 3, guarantee plate-like channel 3 in Ion reagent because
Concentration difference penetrates into always cell culture insert 4, and keeps 4 iso-ionia of cell culture insert.Close all 11 Hes of shut-off valve
It is loaded valve 1.By 12 sets of identical intravascular stent on matched bracket experiment tube 7.Identical culture base flow is added from sample port 8
Enter cell culture insert 4.Identical suspension liquid of living cell is seeded on intravascular stent 12.After cell is adherent, shut-off valve is opened
11, the shut-off valve needs of 8 centre of two of them sample port remain turned-off, and open sample-adding valve 1, different experiment reagents is added (such as
Inflammatory factor and Porcine HGF), Circuluting puls Micropump 6 is adjusted, related experiment can be started.During the experiment, it is loaded
Valve 1 is in close state.According to the state of cell in cell culture insert 4, cell culture fluid is added by sample port 8.It can pass through
Sampling chock plug 13 is sampled analysis, is degraded by experiment front and back support or the adherency and growth of deformation extent, rack surface cell
Experimental data, evaluation experimental result are collected in the variation of ingredient in degree, culture solution.
5 platelet attachment experiment of embodiment
Using rectangle intravascular stent experiment micro-fluidic chip described in embodiment 1, all shut-off valves 11 and sample-adding are closed
Valve 1.Surface is machined with to the intravascular stent 12 of different coating ingredient (coating composition can be dopamine, PEI and drug), is covered
On the bracket experiment tube 7 to match therewith.Identical culture medium is added from sample port 8 and flows into cell culture insert 4.It adds in a device
The platelet rich plasma of comparable sodium can at the uniform velocity flow through rack surface.37 DEG C of experimental temperature, experimental period 1h will be identical
Suspension liquid of living cell be seeded on intravascular stent 12, after cell is adherent, open shut-off valve 11, adjust Circuluting puls Micropump 6,
It can start related experiment.The evaluation and test of subsequent experimental result is carried out using scanning electron microscope (SEM).This time detection is each dense
Degree sample has all randomly choosed the visual field that six amplification factors are 3000 times, as shown in figure 9, A is 2mg/ml dopamine+5mg/ml
PEI group；B is 2mg/ml dopamine+10mg/ml PEI group；C is 2mg/ml dopamine+15mg/ml PEI group；D is blank control
Group does not have cated 316L stainless steel stent group, 1 indicates not carrying medicine group, and 2 be load medicine group.
According to the quantitative analysis of blood platelet in platelet PLA2 and visual field frame it is found that in the production method of mixing film forming,
Carry medicine combination with do not carry medicine group and compare, the negligible amounts of blood platelet in the frame of the visual field, it is often more important that the platelet PLA2 adhered to
There is no excessive changes, stretch out without pseudopodium.In three load medicine groups, B-2 group, that is, 2mg/ml dopamine+10mg/ml
PEI carries medicine combination adherency minimum number, and the platelet PLA2 of adherency rounded normal condition mostly, activation degree is low, small in blood
Effect is relatively preferable in plate adherence test.
The experiment of 6 vascular smooth muscle cell proliferation of embodiment
1. by the brackets of 4 groups of different experiments types (316LSS group, 5mg/ml PEI+DA+GO+DTX coating group,
10mg/ml PEI+DA+GO+DTX coating group and 15mg/ml PEI+DA+GO+DTX coating group) in superclean bench
In ultraviolet front and back sides radiation sterilization 12h；
2. being put into the bracket after sterilizing in the orifice plate, smooth muscle cell suspension is added, cell is allowed to adhere in rack surface；
3. chip sterilizes, above-mentioned branch is taken to be placed in chip, cell culture medium is added, then is placed in 37 DEG C, 5%CO2 culture
It is cultivated in case；
4. chip is at the appointed time taken out (1d, 3d, 5d), takes out bracket and be put into new orifice plate, 500 μ L are added in every hole
Culture medium and 50 μ LMTS detect liquid, then place back in and wet in 37 DEG C of cell incubators incubate 2h；
5. taking out orifice plate, shaking up reaction solution and drawing in 100 μ L, 96 orifice plates of addition, it is placed at microplate reader 490nm wavelength, surveys
Absorbance OD value.
As shown in Figure 10, all exist between three groups of carried stents and the OD value of the naked sheet scaffold of 316LSS in the 1st, 3,5d aobvious
It writes sex differernce (p < 0.05), wherein there are extremely significant property is poor between three groups of carried stents and the naked sheet scaffold of 316LSS in 5d
Different (p < 0.01).The OD value of carried stent illustrates this group of coating DTX (medicine well below not carried stent and naked sheet scaffold
Object) it carries successfully, and significant inhibiting effect is shown to the proliferation of smooth muscle cell.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
Claims (10)
1. a kind of extracorporeal blood vessel bracket evaluation and test micro-fluidic chip, which is characterized in that mainly by sample-adding valve 1, Circuluting puls Micropump
6, cell culture insert 4 forms.
2. micro-fluidic chip according to claim 1, which is characterized in that the chip center position is machined with 2 side by side
Sample-adding valve 1；Each sample-adding valve 1 is machined with 2 opposite openings on same diameter of section line, and each opening is separately connected 1
Sample feeding pipe 2；Each sample feeding pipe 2 respectively leads to a respective cell culture insert 4, totally 4 cell culture inserts 4, and
The plate-like channel 3 processed with bottom in cell culture insert 4 communicates.
3. micro-fluidic chip according to claim 2, which is characterized in that add between the cell culture insert 4 described in a pair
Work is equipped with 1 Circuluting puls Micropump 6, and Circuluting puls Micropump 6 is ganged up among 2 circulation pipes 5,2 circulation pipes 5 respectively with institute
Plate-like channel 3 is stated to be connected.
4. micro-fluidic chip according to claim 3, which is characterized in that the circulation pipe 5 and sample feeding pipe 2 are described thin
It is connected to jointly at the T-type interface 17 on 4 side of born of the same parents' culture pond with plate-like channel 3.
5. micro-fluidic chip according to claim 4, which is characterized in that the plate-like channel 3 is along the cell culture insert 4
Bottom surface is spiraled at the center of cell culture insert 4, is communicated with bracket experiment tube 7, and the bracket experiment tube 7 is to be higher by the cell
The hollow pipe of culture pond 4, is equipped with sampling chock plug 13 at top end opening, equipped with pressure sensing spy inside the sampling chock plug 13
Head.
6. micro-fluidic chip according to claim 1, which is characterized in that sample feeding pipe 2, plate-like channel 3, circulation pipe 5, sample
Channel 9, one-way circulation pipe 10 and bracket experiment tube 7 are all sealing pipelines.
7. a kind of plate-like channel 3 of micro-fluidic chip described in claim 1, which is characterized in that use ion in the plate-like channel 3
Exchange membrane closing.
8. plate-like channel 3 according to claim 7, which is characterized in that there is pipeline partition wall 19 among the plate-like channel 3,
It is divided into 2 plate-like double small pipes 18 from T-type interface 17 by plate-like channel 3.
9. application of the micro-fluidic chip described in any one of claims 1-6 in intravascular stent is evaluated and tested in vitro.
10. application of the amberplex that plate-like channel 3 as claimed in claim 7 uses in intravascular stent is evaluated and tested in vitro.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109097277A (en) * | 2018-09-08 | 2018-12-28 | 重庆科技学院 | A kind of application method of cytotoxicity experiment chip |
| CN109682586A (en) * | 2019-01-14 | 2019-04-26 | 重庆大学 | A kind of methods and applications carrying out the evaluation and test experiment of extracorporeal blood vessel bracket based on micro-fluidic chip |
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| US20070243574A1 (en) * | 2006-01-27 | 2007-10-18 | University Of Arizona | Vascular mimic for drug and device evaluation |
| CN101294131A (en) * | 2007-04-27 | 2008-10-29 | 中国药品生物制品检定所 | Bioreactor for vascellum tissue engineering |
| FR2973146A1 (en) * | 2011-03-25 | 2012-09-28 | Arts | Test bench for testing in-vitro behavior of active pro-healing stent, has centrifugal pump including output connected to supply pipe for feeding test-tubes, where test-tubes are connected to return fluid collector |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20070243574A1 (en) * | 2006-01-27 | 2007-10-18 | University Of Arizona | Vascular mimic for drug and device evaluation |
| CN101294131A (en) * | 2007-04-27 | 2008-10-29 | 中国药品生物制品检定所 | Bioreactor for vascellum tissue engineering |
| FR2973146A1 (en) * | 2011-03-25 | 2012-09-28 | Arts | Test bench for testing in-vitro behavior of active pro-healing stent, has centrifugal pump including output connected to supply pipe for feeding test-tubes, where test-tubes are connected to return fluid collector |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109097277A (en) * | 2018-09-08 | 2018-12-28 | 重庆科技学院 | A kind of application method of cytotoxicity experiment chip |
| CN109097277B (en) * | 2018-09-08 | 2023-07-14 | 重庆科技学院 | Application method of chip for cytotoxicity experiment |
| CN109682586A (en) * | 2019-01-14 | 2019-04-26 | 重庆大学 | A kind of methods and applications carrying out the evaluation and test experiment of extracorporeal blood vessel bracket based on micro-fluidic chip |
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