CN106290176A - The method of testing of the cleaning series products PM2.5 cleaning force to being attached on skin - Google Patents
The method of testing of the cleaning series products PM2.5 cleaning force to being attached on skin Download PDFInfo
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- CN106290176A CN106290176A CN201610872625.6A CN201610872625A CN106290176A CN 106290176 A CN106290176 A CN 106290176A CN 201610872625 A CN201610872625 A CN 201610872625A CN 106290176 A CN106290176 A CN 106290176A
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- skin
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- colourity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
Abstract
Disclosing and a kind of clean the series products method of testing to skin clean power, it comprises the steps: to collect the pollutant in air, is prepared as suspension;Measure colourity a in the human body skin district wanting tested0, above-mentioned suspension is coated in described human body skin district, forms coating zone, and measure colourity a of coating area1;Clean described coating area with described cleaning series products, and measure colourity a of cleaned coating area2;Cleaning force η: η=(a is calculated with following formula1‑a2)/(a1‑a0)。
Description
Technical field
The present invention relates to clean the series products cleaning force method of testing to skin.It is more particularly related to cleaning
The cleaning force method of testing of the series products especially PM2.5 of the small particle to being attached on skin.
Background technology
Haze is also referred to as gray haze (mist and clouds in the twilight), refers to that the particles such as the dust in air, sulphuric acid, nitric acid, organic hydrocarbon compounds make air
Muddiness, the visual field obscures and causes visibility deterioration, this phenomenon to be common in city.Mist is incorporated to haze one and acts as by the many areas of China
Carry out early-warning and predicting for diastrous weather phenomenon, be referred to as " haze weather ".A lot of cities of China all occurred light or heavy
Haze weather.
" air fine particles (PM2.5) and pathogenic present Research thereof " (" clinical and experimental pathology of Yue Changli etc.
Magazine " the 4th phase of volume 25, in August, 2009) describe the source of PM2.5 and physicochemical property and pathogenesis thereof.It can cause exhales
The disease of desorption system, blood circulation and central nervous system
JIUYUE in 2014 within 15th, be published on " medical aesthetics beauty treatment (wealth intelligence) " magazine " the PM2.5 epoch tackle skin " city
City is sick " " literary composition describes the PM2.5 harm to skin:
-when PM2.5 enters skin surface, antibacterial causes inflammation and small pox, heavy metal induction sensitivity, is commonly called as blushing.Example
If the sulfate in PM2.5 is in an atmosphere through sun exposure, easily it is irritating to the skin and corrosiveness, causes skin allergy;
Nitrate can be combined with other pollutant in environment, stimulates skin, mucosa, and even induction leukoderma is sick;
-when PM2.5 enters skin base layer, and it is dark yellow, gloomy that oxide therein and heavy metal etc. can allow skin present, custom
Claim black face.Pore is the main path that PM2.5 enters skin, and PM2.5 enters skin by pore and pore, sebaceous gland can be disturbed to lead
Pipe, sebaceous gland and the eubolism of hair follicle, induce clogging of pores, sebaceous gland duct dyskeratosis, hypersteatosis, hair
Capsule is impaired;
-when PM2.5 enters dermal layer of the skin, oxide can aoxidize collagen protein and elastin laminin, fold, pine occurs
Relax, be commonly called as old face.As sweater can adhere to the visible dust of some in environment, environmental contaminants are attached on skin can be to skin
Skin produces the most potential stimulation and injury.Oxynitride can cause radical reaction and inflammatory reaction, makes extracellular matrix
And cell membrane damage, accelerate the aging of skin.
Obviously, PM2.5 is the process of a gradual change on the impact of skin.Short period (such as 24 hours) is in PM2.5
During air pollution, skin will not produce significantly reflection.The survey result of more than 1000 Chinese womens is shown. more than 95%
Women worry about air pollution at all can affect skin health.
Reducing PM2.5 is to be carried out exposed skin with cleaning series products a kind of method of skin injury.Such as,
" PM2.5 notes skin clean " (" fashion Beijing ", the 4th phase in 2013) of Liu Siyan is mentioned and " wants skin to keep pale the
One step, is deep layer face cleaning naturally, recovers pore cleaning " and it is proposed with pressing type face cleaning product, such as cleansing milk etc..
Having the cleaning series products that various different manufacturers produces at present on the market, light cleansing milk just has tens of kinds.But
There is presently no a kind of reliable method to judge its cleaning effect to PM2.5.
Measure the conventional method that skin color is dermal region.Such as Cheng Ying etc. " skin color objective evaluation method
Relatively " (" clinic skin section magazine ", the 7th phase of volume 34 in 2005) compare two kinds of conventional skin color apparatus measures sides
Method, including using tristimulus color analysis (Chromameter CR200, purchased from the Minolta of Osaka, Japan) and narrow spectrum reflection
Spectrophotometer (Mexameter MX16 and MX18, purchased from Cologne, Germany Courage+Khazaka Electronic company).
Result shows that the repeatability of test is fairly good.Chinese patent application CN105427306A of duty flower bud flower bud etc. discloses a kind of skin light
The image analysis method of pool degree and device, it is included under identical light conditions and gathers the digital picture of subjects skin, meter
Calculate the frequency distribution of pixel, the high light part that the extraction conditions of high light part calculating extracted according to this extraction conditions is set
Pixel accounting is as the index of skin shine.
Although prior art mentions PM2.5 can allow dull-looking skin, but this change is the effect of long-term accumulated, is difficult to use
Measured by conventional skin color or skin shine measures the reflection PM2.5 short-term effect to skin.
Therefore, this area needs a kind of method of testing cleaning the series products PM2.5 cleaning force to being attached on skin, it
There is the advantage that step is simple, quick and with low cost.
Summary of the invention
The present invention provides a kind of method of testing cleaning the series products PM2.5 cleaning force to being attached on skin, and it has
The advantage that step is simple, quick and with low cost.
Therefore, it is an aspect of the invention to provide and a kind of clean the series products PM2.5 cleaning force to being attached on skin
Method of testing, it comprises the steps:
-collect the pollutant in air, it is prepared as suspension;
-mensuration wants colourity a in tested human body skin district0, above-mentioned suspension is coated in described human body skin district, is formed
Coating zone, and measure colourity a of coating area1;With
-clean described coating area with described cleaning series products, and measure colourity a of cleaned coating area2;
-with following formula calculate cleaning force η:
η=(a1-a2)/(a1-a0)。
Another part of the present invention is to provide the method measuring human body skin colourity at test cleaning series products to being attached to
The purposes in PM2.5 cleaning force on skin.
Accompanying drawing explanation
Further illustrate the present invention below in conjunction with the accompanying drawings.In accompanying drawing:
Fig. 1 a-Fig. 1 c be the present invention a preferable example in smear PM2.5 before (Fig. 1 a), smear PM2.5 after (Fig. 1 b)
And the skin pore mirror picture that after cleaning, (Fig. 1 c) is corresponding;
Fig. 2 a-Fig. 2 c be the present invention a preferable example in smear PM2.5 before (Fig. 2 a), smear PM2.5 after (Fig. 2 b)
And the photographic picture that after cleaning, (Fig. 2 c) is corresponding.
Detailed description of the invention
The present invention provides a kind of method of testing cleaning the series products PM2.5 cleaning force to being attached on skin.It is applicable to
The cleaning series products of method of testing of the present invention is without particular limitation, as long as it has cleaning action.Such as, it can be to wash
Face milk, bath gel, soap, there is the facial film of cleaning function, clear water etc..
The inventive method includes collecting the pollutant in air, is prepared as the step of suspension.The present invention collects in air
Pollutant or the method for particulate matter without particular limitation, can be conventional method known in the art.In the present invention one
In individual example, according to national environmental standard HJ93-2013 commercially available samplers sample PM10 and PM2.5 particulate matter.At this
In another bright example, use the BTPM-AS1 intelligent environment air particle sampling purchased from Dandong Bai Te Instrument Ltd.
Device gathers PM2.5 particulate matter.The time gathered is without particular limitation, depends on the atmospheric condition etc. when gathering.In the present invention
An example in, the time of collection is 10-30 hour, preferably 12-28 hour, more preferable 15-20 hour.
The method that the present invention prepares Particulate suspension is without particular limitation, can be conventional method known in the art.
In an example of the present invention, sampler filter membrane being cut into little strip, be placed in beaker, sonic oscillation cleans, after concentration
To required suspension.
In another example of the present invention, sampler filter membrane is cut into little strip, is placed in beaker and uses alcohol submergence, ultrasonic
Ripple shakes, and pours out supernatant, then adds alcohol, continues ultrasonic wave concussion, pours out supernatant and merge with a front supernatant.Filter, flat
All pour multiple centrifuge tube into be centrifuged, PM2.5 deposition and fraction clear liquid 5ml, remaining supernatant altogether bottom every centrifuge tube reservation
Remove, be PM2.5 suspension.
In an example of the present invention, in described PM2.5 suspension, particulate matter accounts for 50-95 mass %, preferably accounts for 60-90
Weight %, more preferably accounts for 70-85 weight %.
Due to PM2.5Suspend and the most invisible also can not touch, be therefore difficult to its deposition on skin is carried out quantitatively
Analyze.The present invention collects the PM2.5 in air by sampling apparatus, and spreads upon human body skin after being prepared into suspension
On, with cleaning series products, it is carried out the most again, assesses clear by the colourity change of skin before and after smearing, before and after cleaning
The clean series products cleaning force to PM2.5, thus realize the quantitative assessment to PM2.5 cleaning force and compare.
Therefore, the inventive method includes colourity a measuring the human body skin district wanting tested0, above-mentioned suspension is coated in
Described human body skin district, forms coating zone, and measures colourity a of coating area1Step.
The present invention is without particular limitation for the method measuring human body skin colourity, can be routine side known in the art
Method.In an example of the present invention, use the tristimulus color analysis (Chromameter purchased from Osaka, Japan Minolta
CR200) colourity a is measured0、a1And a2.In another example of the present invention, use purchased from Cologne, Germany Courage+Khazaka
The narrow-band simple reflectance meters (Mexameter MX16 or MX18) of Electronic company measures colourity a0、a1And a2.At this
Invent in another example, use the Minolta CM-700d purchased from Japan Konica to measure colourity a0、a1And a2。
Area and the coated weight of coating area are without particular limitation, be referred in " cosmetics health specification " (2007) about
The coated area specified in " sun care preparations SPF (sun protection factor) (spf value) assay method " and the applying amount of little 1,000 times.At this
In a bright example, coated area is 1-20cm2, preferable 5-15cm2, more preferable 8-10cm2.In an example of the present invention,
Applying amount is 1-10 μ l/cm2, preferably 2-8 μ l/cm2, more preferably 4-6 μ l/cm2。
In an example of the present invention, the volume unit μ l in described applying amount is to determine its " concentration " with the following method
: sampler filter membrane being cut into the little long treaty 30 of about 3cm × 1cm, is placed in beaker, 80ml ethanol submergence, ultrasound wave shakes
Swing about 30 minutes, pour out supernatant, then add 60ml ethanol, continue ultrasonic wave concussion about 30 minutes, pour out supernatant with front once
Supernatant merges.Described supernatant is filtered through 4 layers of gauze, averagely pours 4 50ml spiral cover centrifuge tubes into, with 10000 revs/min
Centrifugal 20 minutes, PM2.5 deposition and part clear liquid 5ml altogether bottom every centrifuge tube reservation, remaining supernatant was removed, is shaken up merging
4 centrifuge tube PM2.5 deposit liquid 5ml, are PM2.5 suspension.
Requirement to subject can refer in " cosmetics health specification " (2007) about " sun care preparations SPF (sun protection factor)
(spf value) assay method " in regulation.I.e. 18~60 years old man or female healthy volunteer, the skin of recipient site should be colourless
Element calmness, inflammation, cicatrix, nevus cell nevus, hirsutism etc..
The inventive method also includes by following formula calculating cleaning force η:
η=(a1-a2)/(a1-a0)。
The inventive method smears certain density PM on skin2.5After, smear the skin colourity at position relative to not smearing
Position colourity is the most dimmed, and with cleaning series products by PM2.5Color before the colourity at this position returns to close smearing after cleaning
Degree, therefore can assess cleaning series products to PM according to skin aberration Δ E before and after cleaning2.5Cleaning force.
Fig. 1 be the present invention a preferable example in smear PM2.5 before, smear PM2.5 after and cleaning after corresponding skin
Skin pore mirror picture.Relatively above-mentioned picture is visible, and after cleaning, the color of skin is close to the color of skin when not smearing PM2.5.
Fig. 2 be the present invention a preferable example in smear PM2.5 before, smear PM2.5 after and cleaning after corresponding photograph
Picture.By described photographic picture it is also seen that the color of skin is close to the color of skin when not smearing PM2.5 after Qing Jie.
Below in conjunction with embodiment, the present invention done further detailed description, but embodiments of the present invention are not limited to this.
Embodiment
1. preparation PM2.5 suspension
a)PM2.5Collection:
Under outdoor awning, at supporting PM2.5Airborne particulate harvester (the BTPM-AS1 intelligent environment air particles of sickle
Thing sampler, purchased from Dandong Bai Te Instrument Ltd.) on, cellulose mixture filter membrane (A4 paper size) is put into cleaning with tweezers
On filter screen in sampling folder, start gathers about 24 hours total times.
b)PM2.5Eluting:
Take off collection and have PM2.5The filter membrane of atmosphere pollution, is cut into the little long treaty 30 of about 3cm × 1cm, is placed in beaker
In, 80ml ethanol submergence, ultrasonic wave concussion about 30 minutes, pour out supernatant, then add 60ml ethanol, continue ultrasonic wave concussion about 30
Minute, pour out supernatant and merge with a front supernatant.
c)PM2.5The preparation of suspension:
Supernatant is filtered through 4 layers of gauze, averagely pours 4 50ml spiral cover centrifuge tubes into, with 10000 revs/min centrifugal 20
Minute, PM bottom every centrifuge tube reservation2.5Deposition and part clear liquid 5ml altogether, remaining supernatant is removed, and shakes up merging 4 and is centrifuged
Pipe PM2.5Deposition liquid 5ml, is PM2.5Suspension, its solid content is about 85 weight %.
Embodiment 1
Arm part position is carried out described point: measure and record described point area skin Lab with Konica Minolta CM-700d
Value, measuring condition is provided that measured area is MAV;Direct reflection light component pattern is SCI;The colour space is Lab;Viewing angle
It it is 10 degree;Light source is D65.And with pore mirror, described point area skin is taken pictures (Fig. 1 a), to surveyed position general camera
Carry out taking pictures (Fig. 2 a).
Smear PM2.5 suspension obtained above at described point position, applying amount is 5 μ l/cm2, position to be applied is dried
Measure and record and smear area skin Lab value, carry out in neighbouring region three times measuring (due to smear for suspension, at skin
It is distributed the most uniform on skin), and take pictures (Fig. 1 b) to smearing position with pore mirror, surveyed position general camera is carried out
Take pictures (Fig. 2 b).
Rinsing 1 minute with flowing water after 30 minutes, wash away PM2.5, region to be rinsed is the most again measured and records Lab
Value, and take pictures (Fig. 1 c) to smearing position with pore mirror, surveyed position general camera is taken pictures (Fig. 2 c).
According to measuring Lab value, calculate and smear before and after aberration Δ E1=(a1-a0) and clean before and after aberration Δ E2 (a1-
a2), and calculate η value, result the former be 7.35 ± 1.06;The latter is 5.41 ± 0.77 (X ± SD, n=8);η value is 0.736.
Embodiment 2
The present embodiment uses the method in embodiment 1 to assess one group of experimenter in batches and is smearing PM2.5After re-use Tai Ji
Its skin colourity situation of change after mud black mud and clear water cleaning, compares Tai Ji mud black mud and clear water to PM2.5The power of cleaning force.
Volunteer chooses: choose 8 22~36 years old healthy women experimenter, and hands forearm position sinks without obvious cicatrix, pigment
And require that experimenter's colour of skin is whiter;
Described point and packet: opponent's forearm position carries out described point and packet, and often group described point area is 2 × 2cm2, experiment is divided into 3
Group: blank (not smearing PM2.5), clear water (smear above-mentioned PM2.5 suspension and wash with water), Tai Ji mud black mud (are smeared
Above-mentioned PM2.5 suspension also applies 5~10min with black mud, then wash away);
Measure and calculate: according to step in embodiment 1, each position and time point are carried out the measurement of Lab value, i.e. smear
After 30 minutes, clear water group clear water rinses, Tai Ji mud black mud (purchased from Shanghai Fine Chemical Co., Ltd's herborist brand) group
Wait after smearing with Tai Ji mud black mud and clean again for 10 minutes.
According to measuring Lab value, calculate aberration Δ E2 before and after smearing before and after aberration Δ E1 and cleaning.Result such as following table institute
Show:
Skin aberration Δ E (X ± SD, n=8) before and after table 1. is smeared and before and after cleaner
L | Smear before and after aberration Δ E1 | Aberration Δ E2 before and after cleaning | η value |
Blank | 0.72±0.52 | 0.72±0.32 | 1.0 |
Clear water | 7.40±1.15* | 5.39±0.75* | 0.728 |
Tai Ji mud black mud | 7.24±1.60* | 6.21±1.25*▲ | 0.858 |
* represent and have significant difference relative to blank
▲ representative has significant difference relative to clear water
P < 0.05 represents that difference is statistically significant
Before and after smearing PM2.5 as shown in Table 1, skin aberration Δ E1 has significant difference, explanation to smear with blank group ratio
After PM2.5, its skin colourity occurs significantly to change, and between clear water group and Tai Ji mud black mud group before and after smearing PM2.5 aberration
Δ E1 without significant difference, illustrates to smear for two groups the aberration that PM2.5 brings basically identical;And use clear water and Tai Ji mud
After black mud cleaning, aberration Δ E2 before and after cleaning, there are significant difference, and the aberration Δ E2 that Tai Ji mud black mud group is corresponding with blank group
Bigger than clear water group, show that clear water and Tai Ji mud black mud have effect of cleaning PM2.5, and effect of Tai Ji mud black mud is better than clearly
Water, thus the method can detect the cleaning series products cleaning force to PM2.5, and can distinguish different cleaning series products
Size to PM2.5 cleaning force.
Claims (10)
1. cleaning a series products method of testing to skin clean power, it comprises the steps:
-collect the pollutant in air, it is prepared as suspension;
-mensuration wants colourity a in tested human body skin district0, above-mentioned suspension is coated in described human body skin district, forms coating
Region, and measure colourity a of coating area1;With
-clean described coating area with described cleaning series products, and measure colourity a of cleaned coating area2;
-with following formula calculate cleaning force η:
η=(a1-a2)/(a1-a0)。
2. method of testing as claimed in claim 1, it is characterised in that the step of described supending includes: filtered by sampler
Film is cut into little strip, is placed in beaker, and sonic oscillation is the most de-, obtains required suspension after concentration.
3. method of testing as claimed in claim 1, it is characterised in that the step of described supending includes: filtered by sampler
Film is cut into little strip, is placed in beaker and uses alcohol submergence, ultrasonic wave concussion, pours out supernatant, then adds alcohol, continues ultrasonic wave concussion,
Pour out supernatant to merge with a front supernatant, filter, averagely pour multiple centrifuge tube into and be centrifuged, bottom every centrifuge tube reservation
PM2.5 deposition and fraction clear liquid 5ml altogether.
4. method of testing as claimed in claim 3, it is characterised in that: sampler filter membrane is cut into the little strip of about 3cm × 1cm
About 30, it is placed in beaker, 80ml ethanol submergence, ultrasonic wave concussion about 30 minutes, pours out supernatant, then add 60ml ethanol, continue
Continuous ultrasonic wave concussion about 30 minutes, pours out supernatant and merges with a front supernatant;Described supernatant is filtered through 4 layers of gauze,
Averagely pouring 4 50ml spiral cover centrifuge tubes into, be centrifuged 20 minutes with 10000 revs/min, bottom every centrifuge tube reservation, PM2.5 sinks
Long-pending and part clear liquid is total to 5ml, and remaining supernatant is removed, and shakes up 4 centrifuge tube PM2.5 of merging and deposits liquid 5ml, is PM2.5 and hangs
Supernatant liquid.
5. the method for testing as according to any one of claim 1-4, it is characterised in that the area of coating zone is 1-20cm2, relatively
Good 5-15cm2, more preferable 8-10cm2。
6. the method for testing as according to any one of claim 1-4, it is characterised in that the applying amount of coating zone is 1-10 μ l/
cm2, preferably 2-8 μ l/cm2, more preferably 4-6 μ l/cm2。
7. the method for testing as according to any one of claim 1-6, it is characterised in that use Konica Minolta CM-
700d measures and records skin colourity.
8. the method for testing as described in any one of claim 1-7, it is characterised in that the pollutant in described air are PM2.5
Grain thing.
9. the method for testing as according to any one of claim 1-8, it is characterised in that in described suspension, particulate matter accounts for 50-95
Quality %, preferably accounts for 60-90 weight %, more preferably accounts for 70-85 weight %.
10. the method measuring human body skin colourity cleans series products to the purposes in skin clean power in test.
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CN114324767A (en) * | 2021-12-30 | 2022-04-12 | 广州宏度精细化工有限公司 | Method for evaluating cleaning ability of skin cleaning product |
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