CN106265122A - A kind of astringent containing Cer NS & carnosine submicron liposome and preparation method thereof - Google Patents

Abstract

The present invention relates to the technical field of carrier system in cosmetics technology of preparing, more particularly to a kind of astringent containing Cer NS & carnosine submicron liposome and preparation method thereof.A kind of astringent containing Cer NS & carnosine submicron liposome, containing described Cer NS & carnosine submicron liposome in this astringent, described Cer NS & carnosine submicron liposome is made up of active component Cer NS, carnosine and lipid, emulsifying agent, co-emulsifier, stabilizer, preservative and water, in light yellow translucent outward appearance.Astringent containing Cer NS & carnosine submicron liposome of the present invention, has obvious anti-oxidation efficacy with having and significantly improves wrinkle volume and the degree of depth, improve the anti-aging effects of skin surface texture skin.

Description

A kind of astringent containing Cer NS & carnosine submicron liposome and preparation thereof Method

Technical field

The present invention relates to the technical field of carrier system in cosmetics technology of preparing, contain more particularly to one Astringent of Cer NS & carnosine submicron liposome and preparation method thereof.

Background technology

Ceramide also known as sphingolipid, is the work that is made up of sphingosine long chain base and a fatty acid of a class Property composition, plays an important role in skin protection and hair care art.Research shows, ceramide plays firm screen at skin surface Barrier effect, prevents moisture from distributing and to outside stimulation, moist, moisturizing skin.When dry, desquamation, cracking phenomena occurs in skin, When its barrier function substantially reduces, skin supplements ceramide can recover rapidly moisturizing and barrier function.And different nerve sheath Ammonia alcohol base can combine again with fatty acid and form different ceramides.As a member therein, Cer NS is except having Help skin pins moisture, promotes outside skin barrier self-regeneration and regulation and control Skin Cell effect, moreover it is possible to suppression skin enlivens Smegma, makes skin water and oil balance, strengthens the self-protection function of skin.Thus cosmetic field is had by Cer NS There is important both effectiveness value.

Carnosine, by a kind of dipeptide structure being made up of Beta-alanine and L-Histidine, has activity of fighting against senium.At cosmetics In, the activity of fighting against senium mechanism of carnosine is mainly passed through: delay the shortening of telomere；Stop oxidative damage, itself be that activity is free Base (ROS) quencher, is again a kind of heavy metal chelant；Suppression glycosylation, carbonyl radical (RCS) quencher, also may be used Cancellation glycosylation end (AGE) product；Stop macromolecules cross-linking, such as collagen protein；Suppression MMP-1, protection Skin Cell from The damage of UVB.But, as a kind of water miscible dipeptides, its transdermal capability is poor, therefore in the application of anti-aging cosmetics It is restricted.

Nano-carrier technology is that cosmetic industry provides the newest thinking of development.Such as, will have the highest physiology phase The matrix material of capacitive is as carrier, and becoming Nano grade by advanced nanotechnology can the carrier system of carrying active substance System.Wherein, solid lipid nanoparticle (Solid Lipid Nanoparticles) is to grow up early 1990s, Using the degradable naturally occurring or synthetic lipoids material of organism as carrier, by Drug absorbability, wrap up or clamp in lipid film The particle diameter made is about the submicron transport system of new generation of 10-1000 nm, also referred to as submicron liposome.Utilize this carrier Technology is possible not only to improve active substance dissolubility in water and stability, improves its compatibility in formula, moreover it is possible to Improve slow-releasing and the bioavailability of active substance, strengthen its effect performance in cosmetics.Therefore, wrapped up by carrier It is possible not only to weaken even solution Cer NS these shortcomings in application process, the most significantly expands its range of application.Simultaneously Also make Cer NS and carnosine compound to be possibly realized.

Summary of the invention

For improving Cer NS technical problem in application, and the problem that carnosine transdermal rate is low, the present invention's First purpose provides a kind of astringent containing Cer NS & carnosine submicron liposome, its objective is at cosmetics raw In product easy to use, simultaneously can more preferable effect of performance defying age anti-wrinkle in cosmetics.Second object of the present invention It is to provide the preparation method of above-mentioned submicron liposome.

For reaching above-mentioned first purpose, technical scheme is as follows:

A kind of astringent containing Cer NS & carnosine submicron liposome, containing described Cer NS & in this astringent Carnosine submicron liposome, Cer NS & carnosine submicron liposome described by mass percentage is by following component Constitute:

Cer NS 1%-5%,

Carnosine 1%-8%,

Lipid 15%-35%,

Emulsifying agent 10%-30%,

Co-emulsifier 1%-4%,

Stabilizer 0.2%-1.5%,

Preservative 0.2～1.5%,

Remaining is water.

As preferably, Cer NS & carnosine submicron liposome described by mass percentage is by following component Constitute:

Cer NS 2%-4%,

Carnosine 2%-7%

Lipid 20%-30%,

Emulsifying agent 15%-25%,

Co-emulsifier 2%-3%,

Stabilizer 0.5%-1.2%,

Preservative 0.5～1.2%,

Remaining is water.

As improving further, the mean diameter of the Cer NS & carnosine submicron liposome of the present invention is 50 ~ 200nm。

As preferably, described lipid is selected from one or more combinations following: caprylic/capric triglyceride, glycerol acetic acid Ester, glyceryl stearate, butyl capryl alcohol salicylate, glycerin polyether-26, octyl dodecanol.

As preferably, described emulsifying agent is selected from the combination of one or more of: PEG-8 caprylic/capric glyceride type, Stereth-21, ceteth-25, PPG-4-ceteth-20, PEG-30 dimerization hydroxy stearic acid ester, PEG- 40 castor oil hydrogenated, polyglycerolpolyrici.oleate, Pyrusussuriensis smooth olive oil acid esters, the smooth oleate of Pyrusussuriensis, the smooth laurate of Pyrusussuriensis, Sorbitan sesquioleate, Polyoxyethylene Sorbitan Monooleate, polysorbate-60, polysorbate-40, Coco-Glucoside/cocos nucifera oil Alcohol, behenyl alcohol and Natrulon H-10 five stearate and stearoyl dilactic acid sodium mixture.

As preferably, described co-emulsifier is selected from the combination of one or more of: olive oil PEG-7 esters, poly-sweet Oil-3 diisopstearates, ceteth-6.

As preferably, described stabilizer is selected from the combination of one or more of: butanediol, dipropylene glycol, spermol, fourth Hydroxy toluene.

As preferably, described preservative is selected from the combination of one or more of: phenoxyethanol, Sensiva SC50, Buddhist nun Pool tortoise beetle ester, propylparaben.

For reaching above-mentioned second purpose, technical scheme is as follows:

A kind of method of the submicron liposome prepared described in any one technical scheme above-mentioned, the method comprises the following steps:

Step (1): using lipid, emulsifying agent, co-emulsifier and stabilizer as oil phase, under the conditions of 60～80 DEG C, mixing is all, 100～300 rpm stirring 10～25 min form uniform mixed solution；

Step (2): the mixed liquor in step (1) adds Cer NS and continues stirring, and mixing time is about 5～15 Min, makes Cer NS be completely dissolved；

Step (3): adding room temperature and the aqueous phase dissolved with carnosine in the mixed solution of step (2), 100～200 rpm constant speed are stirred Mix 5～10 min；

Step (4): be cooled to by step (3) gained mixed liquor less than 45 DEG C, addition 0.5～the preservative of 1.0%, cooling obtains Light yellow translucent Cer NS & carnosine submicron liposome；

Step (5): the Cer NS & carnosine submicron liposome of preparation is joined other materials mixing mixed of astringent Compound uniformly mixes, it is thus achieved that containing the astringent of Cer NS & carnosine submicron liposome.

As preferably, the other materials of described astringent include wetting agent, skin moistening oils and fats, surfactant, thickening agent, One or more mixing in skin conditioning agent, antiinflammatory, deionized water.

By technique scheme, a kind of cosmetic containing Cer NS & carnosine submicron liposome that the present invention provides Water and preparation method thereof, it has the beneficial effects that: liposome possesses the spy that stability is high, water dispersible is good, bio-compatibility is good Point, can interworking any with astringent formula, and preparation method is the most controlled, reproducible.The more important thing is, containing ceramide The astringent of 2 & carnosine submicron liposomes have skin moisturizing, anti-oxidation efficacy and having is significantly improved wrinkle volume with The degree of depth, improves the anti-aging effects of skin surface texture.

Accompanying drawing explanation

The variation diagram of Fig. 1 wrinkle volume, wherein: * * represents difference highly significant (P < 0.01).

Fig. 2 maximum wrinkle depth variation diagram.

Detailed description of the invention

Below in conjunction with specific embodiment, technical scheme is clearly and completely described.

Embodiment 1

Weigh 5g caprylic/capric triglyceride, 18g octyl dodecanol, 2g tristerin, 2g olive oil PEG-7 esters, 5g polysorbate-60, the smooth laurate of 3g Pyrusussuriensis, 15g PEG-30 dimerization hydroxy stearic acid ester and 5g butanediol, in 65 DEG C, under the conditions of 200 rpm stirring form homogeneous solution；It is subsequently added 2g Cer NS, continues stir about 10 min, add molten There are the aqueous solution of 2g carnosine, 250 rpm to stir 8 min, suitably add 0.5g phenoxyethanol after cooling, continue stirring 5min, cold But solid state gelatineous Cer NS & carnosine submicron liposome is obtained.Measure its particle diameter through laser particle analyzer and be about 103 nm.

Embodiment 2

Weigh 5g tristerin, 5g butyl capryl alcohol salicylate, 22g octyl dodecanol, 5g ceteth-25,5g mountain Pears smooth olive oil acid esters, 5g Polyoxyethylene Sorbitan Monooleate, 10g PEG-30 dimerization hydroxy stearic acid ester, 4g dipropylene glycol and 2g gather sweet Oil-3 diisopstearates, stir into homogeneous solution under the conditions of 70 DEG C, add 1.5g Cer NS, and mixing speed is 300 Rpm, mixing time is 8 min；Adding the 3g carnosine and 32g water dissolved in advance, 200 rpm stir 10 min, suitably cool down Rear addition 0.5g phenoxyethanol and Sensiva SC50 mixture.Natural cooling obtains micro white Cer NS & carnosine sub-micro Mizhi plasmid solid.Measure its particle diameter through laser particle analyzer and be about 125 nm.

Embodiment 3

Weigh 3g caprylic/capric triglyceride, 2 g glyceryl monostearates, 2g butyl capryl alcohol salicylate, 23g octyl group 12 Alcohol, 8g stereth-21,5g PEG-30 dimerization hydroxy stearic acid ester, 2g Coco-Glucoside/cocoanut oil alcohol, 7g behenyl alcohol/ Natrulon H-10 five stearates/stearoyl dilactic acid sodium mixture, 3g butanediol, 3g Polyoxyethylene Sorbitan Monooleate and 2g Pyrusussuriensis Smooth oleate, under the conditions of 80 DEG C, stirring forms homogeneous mixture solotion, adds 2g Cer NS, and mixing speed is 300 rpm, Mixing time is 5 min；It is subsequently added and is pre-dissolved 34g deionized water, 3g carnosine and 0.5g methyl hydroxybenzoate, 200 rpm completely Stirring 8 min, suitably add 0.5g phenoxyethanol after cooling, natural cooling obtains Cer NS & carnosine submicron liposome. This Cer NS & carnosine submicron liposome solid measures its particle diameter through laser particle analyzer and is about 89 nm.

Embodiment 4

The Cer NS & carnosine submicron liposome utilizing the present invention to prepare prepares cosmetic makeup water, and its step is as follows: take Existing astringent formula 89.4g mixes, and adds 10g Cer NS & carnosine submicron liposome (from embodiment 1), 300rpm stirs 15 minutes, is uniformly dispersed；200rpm stirring is cooled to 40 ~ 45 DEG C, adds 0.6g preservative, continues stirring cooling To not higher than 38 DEG C, stop stirring, obtain 100g and contain the astringent of Cer NS & carnosine submicron liposome.

Embodiment 5

The Cer NS & carnosine submicron liposome utilizing the present invention to prepare prepares astringent, and its step is as follows: having of enchashment Adornment water formula 79.4g mixes, and 20g Cer NS & carnosine submicron liposome (from embodiment 3) is heated to 60 ~ 65 DEG C, 300rpm stirs 10 minutes, is uniformly dispersed；200rpm stirring is cooled to 40 ~ 45 DEG C, adds 0.6g preservative, continues stirring cooling To not higher than 38 DEG C, stop stirring, obtain 100g and contain the astringent of Cer NS & carnosine submicron liposome.

Embodiment 6

The frost provided in this experimental selection embodiment 4 carries out clinical experiment.

This experiment includes 20 volunteers, female age in 22-55 one full year of life, the face canthus person that has wrinkle.During testing Use according to regulations tested material and all information.Every day, twice left and right face was smeared at random containing Cer NS & carnosine sub-micro sooner or later The astringent of Mizhi plasmid and the astringent without Cer NS & carnosine submicron liposome, smear for test group, otherwise For matched group.Test period is 6 weeks.In W0(use before), W2 (using 2 weeks), W4(use 4 weeks) and W6(use 6 weeks) pass through Primoslite 18*13 obtains canthus wrinkle view data, and by the volume of software analysis canthus wrinkle and change in depth, and Its diversity is checked with t inspection.Calculating rate of change %, formula is as follows simultaneously:

Rate of change %=(WN-W0)/W0*100, wherein WN refers to use the numerical value in N week, W0 to refer to the numerical value before using.

1. wrinkle bulking value rate of change

It will be noted from fig. 1 that test group and matched group have declined at W2, W4, but test group fall be all better than right According to group, matched group is follow-up simultaneously all bounce-back, and wrinkle volume is deepened when W6, and test group is in W4 best results, and at W6 Good effect can be kept；Knowable to SPSS significant difference, experimental group when W2, W4 and W6 wrinkle volume with use before have bright Significant difference different (P < 0.05), illustrates that the astringent containing Cer NS & carnosine submicron liposome has the work improving wrinkle volume With.

2. maximum wrinkle depth rate of change

From in Fig. 2, test group is all decreased obviously at W2, W4 and W6, although matched group has declined, but the lower range of decrease Degree is weaker than test group.Explanation & carnosine submicron liposome astringent Han Cer NS has the work improving wrinkle depth With.

A kind of astringent containing Cer NS & carnosine submicron liposome that the present invention provides, its Cer NS & flesh Peptide submicron liposome, not only has anti-oxidation efficacy, also has and significantly improve wrinkle volume and the degree of depth, improve skin surface stricture of vagina The anti-aging effects of reason.Meanwhile, this liposome possesses the feature that stability is high, water dispersible is good, bio-compatibility is good, can be with change Any interworking of cosmetic formula.Additionally, the Cer NS & carnosine submicron liposome preparation method of this project is the most controlled, weight Renaturation is good.

The described above of disclosed embodiment of this invention, makes professional and technical personnel in the field be capable of or uses this Bright.Multiple amendment to these embodiments will be apparent from for those skilled in the art, is determined herein The General Principle of justice can realize without departing from the spirit or scope of the present invention in other embodiments.Therefore, originally Invention is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein and features of novelty The widest consistent scope.

Claims (10)

1. the astringent containing Cer NS carnosine submicron liposome, it is characterised in that containing described in this astringent Cer NS & carnosine submicron liposome, this described Cer NS & carnosine submicron lipid by mass percentage Grain is made up of following component:

Cer NS 1%-5%,

Carnosine 1%-8%,

Lipid 15%-35%,

Emulsifying agent 10%-30%,

Co-emulsifier 1%-4%,

Stabilizer 0.2%-1.5%,

Preservative 0.2～1.5%,

Remaining is water.

The astringent of a kind of Cer NS & carnosine submicron liposome the most according to claim 1, it is characterised in that press Cer NS & carnosine submicron liposome described in mass percent is made up of following component:

Cer NS 2%-4%,

Carnosine 2%-7%

Lipid 20%-30%,

Emulsifying agent 15%-25%,

Co-emulsifier 2%-3%,

Stabilizer 0.5%-1.2%,

Preservative 0.5～1.2%,

Remaining is water.

A kind of astringent containing Cer NS & carnosine submicron liposome the most according to claim 1 and 2, its feature Being, the mean diameter of the Cer NS & carnosine submicron liposome stated is 50 ~ 200 nm.

A kind of astringent containing Cer NS & carnosine submicron liposome the most according to claim 1 and 2, its feature Being, described lipid is selected from one or more following combinations: caprylic/capric triglyceride, glyceryl acetate, glyceryl stearate Acid esters, butyl capryl alcohol salicylate, glycerin polyether-26, octyl dodecanol.

A kind of astringent containing Cer NS & carnosine submicron liposome the most according to claim 1 and 2, its feature Being, described emulsifying agent is selected from one or more combinations following: PEG-8 caprylic/capric glyceride type, stereth- 21, ceteth-25, PPG-4-ceteth-20, PEG-30 dimerization hydroxy stearic acid ester, PEG-40 hydrogenated castor Oil, polyglycerolpolyrici.oleate, Pyrusussuriensis smooth olive oil acid esters, the smooth oleate of Pyrusussuriensis, the smooth laurate of Pyrusussuriensis, Pyrusussuriensis smooth sesquialter oil Acid esters, Polyoxyethylene Sorbitan Monooleate, polysorbate-60, polysorbate-40, Coco-Glucoside/cocoanut oil alcohol, behenyl alcohol and poly- Glycerol-10 five stearate and stearoyl dilactic acid sodium mixture.

A kind of astringent containing Cer NS & carnosine submicron liposome the most according to claim 1 and 2, its feature Being, described co-emulsifier is selected from one or more combinations following: olive oil PEG-7 esters, the different tristearin of polyglycereol-3 two Acid esters, ceteth-6.

A kind of astringent containing Cer NS & carnosine submicron liposome the most according to claim 1 and 2, its feature Being, described stabilizer is selected from one or more combinations following: butanediol, dipropylene glycol, spermol, butylated hydroxytoluene；Described Preservative is selected from one or more combinations following: phenoxyethanol, Sensiva SC50, methyl hydroxybenzoate, propylparaben.

A kind of astringent containing Cer NS & carnosine submicron liposome the most according to claim 1 and 2, its feature Being, this astringent is defying age and/or anti-wrinkle astringent.

9. the method for the astringent prepared described in claim 1 ~ 8 any one claim, it is characterised in that the method Comprise the following steps:

Step (1): using lipid, emulsifying agent, co-emulsifier and stabilizer as oil phase, under the conditions of 60～80 DEG C, mixing is all, 100～300 rpm stirring 10～25 min form uniform mixed solution；

Step (2): the mixed liquor in step (1) adds Cer NS and continues stirring, and mixing time is about 5～15 Min, makes Cer NS be completely dissolved；

Step (3): adding room temperature and the aqueous phase dissolved with carnosine in the mixed solution of step (2), 100～200 rpm constant speed are stirred Mix 5～10 min；

Step (4): be cooled to by step (3) gained mixed liquor less than 45 DEG C, addition 0.5～the preservative of 1.0%, cooling obtains Light yellow translucent Cer NS & carnosine submicron liposome；

Step (5): the Cer NS & carnosine submicron liposome of preparation is joined other materials mixing mixed of astringent Compound uniformly mixes, it is thus achieved that containing the astringent of Cer NS & carnosine submicron liposome.

The preparation method of astringent the most according to claim 9, it is characterised in that the other materials of astringent includes moisturizing One or more mixing in agent, skin moistening oils and fats, surfactant, thickening agent, skin conditioning agent, antiinflammatory, deionized water.