CN106262898A - Polypeptide capsule of prevention copper deficiency disease and preparation method thereof - Google Patents
Polypeptide capsule of prevention copper deficiency disease and preparation method thereof Download PDFInfo
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- CN106262898A CN106262898A CN201610683089.5A CN201610683089A CN106262898A CN 106262898 A CN106262898 A CN 106262898A CN 201610683089 A CN201610683089 A CN 201610683089A CN 106262898 A CN106262898 A CN 106262898A
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- intestinum stichopi
- stichopi japonici
- extract
- copper
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- 239000002775 capsule Substances 0.000 title claims abstract description 49
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 39
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 36
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 35
- 206010010957 Copper deficiency Diseases 0.000 title claims abstract description 30
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 30
- 230000002265 prevention Effects 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000000284 extract Substances 0.000 claims abstract description 54
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 36
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910052802 copper Inorganic materials 0.000 claims abstract description 32
- 239000010949 copper Substances 0.000 claims abstract description 32
- 238000001728 nano-filtration Methods 0.000 claims abstract description 23
- 244000241838 Lycium barbarum Species 0.000 claims abstract description 13
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 13
- 235000015468 Lycium chinense Nutrition 0.000 claims abstract description 13
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 13
- 235000020710 ginseng extract Nutrition 0.000 claims abstract description 13
- 238000012545 processing Methods 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 239000007943 implant Substances 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 48
- 102000004190 Enzymes Human genes 0.000 claims description 31
- 108090000790 Enzymes Proteins 0.000 claims description 31
- 239000007788 liquid Substances 0.000 claims description 29
- 238000003756 stirring Methods 0.000 claims description 25
- 108091005804 Peptidases Proteins 0.000 claims description 24
- 239000004365 Protease Substances 0.000 claims description 24
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 23
- 239000012528 membrane Substances 0.000 claims description 21
- 239000012466 permeate Substances 0.000 claims description 21
- 239000006228 supernatant Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000010792 warming Methods 0.000 claims description 15
- 229920001503 Glucan Polymers 0.000 claims description 14
- 238000000502 dialysis Methods 0.000 claims description 14
- 230000000694 effects Effects 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 14
- 235000013305 food Nutrition 0.000 claims description 14
- 102000004882 Lipase Human genes 0.000 claims description 13
- 108090001060 Lipase Proteins 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 239000004367 Lipase Substances 0.000 claims description 12
- 235000019421 lipase Nutrition 0.000 claims description 12
- 239000000725 suspension Substances 0.000 claims description 11
- 108090000145 Bacillolysin Proteins 0.000 claims description 9
- 102000035092 Neutral proteases Human genes 0.000 claims description 9
- 108091005507 Neutral proteases Proteins 0.000 claims description 9
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 7
- 239000012895 dilution Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 230000002779 inactivation Effects 0.000 claims description 7
- 238000012423 maintenance Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 230000004044 response Effects 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- 239000000796 flavoring agent Substances 0.000 claims description 4
- 235000019634 flavors Nutrition 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- ORTQZVOHEJQUHG-UHFFFAOYSA-L copper(II) chloride Chemical compound Cl[Cu]Cl ORTQZVOHEJQUHG-UHFFFAOYSA-L 0.000 claims description 3
- 230000000968 intestinal effect Effects 0.000 claims description 3
- 241000965254 Apostichopus japonicus Species 0.000 claims description 2
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- 235000009508 confectionery Nutrition 0.000 claims 3
- 238000001816 cooling Methods 0.000 claims 2
- 239000000463 material Substances 0.000 claims 1
- 239000012535 impurity Substances 0.000 abstract description 7
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 230000000050 nutritive effect Effects 0.000 abstract description 6
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 229910052751 metal Inorganic materials 0.000 abstract description 4
- 239000002184 metal Substances 0.000 abstract description 4
- 230000035764 nutrition Effects 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 2
- 230000009469 supplementation Effects 0.000 abstract description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 10
- 235000003969 glutathione Nutrition 0.000 description 10
- 229960003180 glutathione Drugs 0.000 description 10
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 241000251511 Holothuroidea Species 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 235000014103 egg white Nutrition 0.000 description 3
- 210000000969 egg white Anatomy 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- UGWKCNDTYUOTQZ-UHFFFAOYSA-N copper;sulfuric acid Chemical compound [Cu].OS(O)(=O)=O UGWKCNDTYUOTQZ-UHFFFAOYSA-N 0.000 description 2
- 238000011033 desalting Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 229910052720 vanadium Inorganic materials 0.000 description 2
- GPPXJZIENCGNKB-UHFFFAOYSA-N vanadium Chemical compound [V]#[V] GPPXJZIENCGNKB-UHFFFAOYSA-N 0.000 description 2
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical compound C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 208000000718 duodenal ulcer Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000003867 tiredness Effects 0.000 description 1
- 208000016255 tiredness Diseases 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to a kind of polypeptide capsule preventing copper deficiency disease and preparation method thereof, the implant of described polypeptide capsule is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extract chelated copper 10 20 parts, Radix Ginseng extract 10 20 parts, wolfberry fruit extract 5 10 parts, the preparation method of Intestinum Stichopi japonici extract chelated copper comprises the steps: the first step, prepares Intestinum Stichopi japonici extract (1) Intestinum Stichopi japonici pretreatment；(2) homogenizing of Intestinum Stichopi japonici；(3) primary enzymolysis；(4) secondary enzymolysis；(5) ultrafiltration；(6) nanofiltration；Second step, chelating.The polypeptide capsule rich in nutrition content of prevention copper deficiency disease prepared by the present invention, it is easy to absorbing, absorption rate is high, and impurity and content of beary metal are low, are of high nutritive value, and both can supplement again the multiple nutrient coming from Intestinum Stichopi japonici with supplementation with copper element.
Description
Technical field
The present invention relates to a kind of polypeptide capsule preventing copper deficiency disease and preparation method thereof.
Background technology
Stichopus japonicus belongs to one of precious seafood, and it is internal contains more than the 50 kind of nutritional labeling useful to human physiological activity, wherein
Protein content is higher, trace element abundant species, it is possible to continuity human senility, tonifies Qi of the kidney, essence-replenishing and marrow-strengthening, allaying tiredness,
Have more anticoagulation, antitumor, antibacterial, antiviral and improve the effect of immunity.In recent years, the enterprise of processing sea cucumber the most more comes
The most, contained in the leftover bits and pieces-Intestinum Stichopi japonici during Holothurian machining nutritional labelings, no less than body wall.Its dry intestinal rate Han vanadium
It is 12 parts of million parts, higher than the rate Han vanadium in its body 3 times.It has effect of warming middle-Jiao and tonifying deficiency pain relieving, can treat stomach and ten
Two Duodenalulcers.
The utilization of Intestinum Stichopi japonici resource day by day causes attention, after Intestinum Stichopi japonici is manually removed sand by a lot of enterprises, cleans, cold air drying
Dry or lyophilization, then polishing or micronizing, make capsule product.But Intestinum Stichopi japonici is not done life by this kind of capsule product
Thing processes, and absorption rate is low, and nutritive value is had a greatly reduced quality.It addition, there is also in the process of raw material: manually going of Intestinum Stichopi japonici
Husky mud efficiency is low and silt is thorough, thorough with water cleaning, desalting, causes what content of beary metal and salinity in product exceeded standard to ask
Topic.
The elements such as iodine, copper, phosphorus are the indispensable elements of human body, Deficiency of Intake can affect healthy, especially copper
Lacking, can cause copper deficiency disease, Intestinum Stichopi japonici is extracted and obtains extract by the present invention, then chelates with copper and mixes with other adjuvant
Close, glue capsule, have benefit copper function concurrently, it is also possible to the efficient supplementary multiple nutrient come from Intestinum Stichopi japonici, can be fabulous
Prevention copper deficiency disease.
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, it is provided that a kind of polypeptide capsule preventing copper deficiency disease and
Preparation method, the polypeptide capsule rich in nutrition content of prevention copper deficiency disease prepared by the method, it is easy to absorb, impurity and weight
Tenor is low.
The technical solution adopted for the present invention to solve the technical problems is:
The polypeptide capsule of prevention copper deficiency disease, the implant of described polypeptide capsule is by the Raw material processing of following weight portion
Become: Intestinum Stichopi japonici extract chelated copper 10-20 part, Radix Ginseng extract 10-20 part, wolfberry fruit extract 5-10 part, Intestinum Stichopi japonici extract
The preparation method of chelated copper comprises the steps:
The first step, prepares Intestinum Stichopi japonici extract (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A
Carrying out pretreatment with B, pretreating agent A is the water-insoluble glucan suspension of mass fraction 8-12%, and described pretreating agent B is
The reduced glutathion aqueous solution of mass fraction 0.5-1.5%, concrete pretreatment operation is: first use pretreating agent A in often
The lower pretreatment 1-2h of temperature, solid-liquid ratio is 1:2-3, then stirs pretreatment 2-3h under room temperature with pretreating agent B, then stands
45min-1h, solid-liquid ratio is 1:1-2, or is mixed homogeneously with volume ratio 1:1 with pretreating agent B by pretreating agent A, is subsequently adding
Fresh Intestinum Stichopi japonici, stir process 2-2.5h under room temperature, then stand 0.5-1h, solid-liquid ratio 1:2, solid-liquid after pretreatment
Separate standby；
Rich in silt, heavy metal, salinity in Intestinum Stichopi japonici, want to obtain the Intestinum Stichopi japonici extract of high-quality, need fully to go
Except above-mentioned impurity, the design concept of the present invention is as follows: (1) water-insoluble glucan suspension is the aqueous suspension of macromole, greatly
Molecule glucosan itself has the effect of flocculation of reuniting, and can fully adsorb or the mud that flocculates in Intestinum Stichopi japonici and beavy metal impurity etc.
The removal effect of impurity, especially heavy metal is splendid, after using glucosan suspension pretreating agent pretreatment, and stretching of Intestinum Stichopi japonici
Malleability is the most excellent, and follow-up homogenizing, the effect of enzymolysis are substantially improved, it addition, glucosan safety non-toxic, the glucosan of residual has on the contrary
Increase immunity, improve effect of immunologic function, it is provided that the nutritive value of Intestinum Stichopi japonici extract；(2) reduced glutathion water
Solution has the function of good activating cell and tissue, can improve the active oxygen in cell or tissue and osmotic pressure, maintains
Intestinum Stichopi japonici cell and the fresh and healthy state of tissue long period, be substantially improved the effect of follow-up ferment treatment, and glutathion is also pacified
Atoxic, the glutathion of residual has the function improving immune system, removing toxic substances on the contrary, improves the nutriture value of Intestinum Stichopi japonici extract
Value, improves the quality of finished product, Heavy Metal Pollution risk that may be present in Intestinum Stichopi japonici extract is reduced further or eliminated；Separately
Outward, the reduced glutathion of residual plays the effect of antioxidant, and capsule quality is stable, long shelf-life, edible safety；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer, remove the fat in Intestinum Stichopi japonici by lipase enzymolysis
Fat, can improve the quality of Intestinum Stichopi japonici extract, extends the shelf-life of Intestinum Stichopi japonici extract；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant, logical
Cross enzymolysis and the protein in Intestinum Stichopi japonici be fully degraded into aminoacid and micromolecule polypeptide, it is simple to the absorption of Intestinum Stichopi japonici extract with
Utilizing, utilization rate is high, is of high nutritive value；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is for 150-300Da, by nanofiltration to Intestinum Stichopi japonici extract desalting processing, it is thus achieved that Intestinum Stichopi japonici extract salinity low, in good taste；
Second step, chelating:
Under conditions of 50-60 DEG C, in nanofiltration concentrated solution, add suitable quantity of water dissolubility mantoquita while stirring, the most slowly adjust
Joint system pH is 5.5-6.5, and the response time is 2～3h, maintenance system pH stable in course of reaction, reaction terminate after cool down,
Filtering, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated copper.
Described mantoquita is copper sulfate and/or copper chloride.
Preferably, described capsule is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extract chelated copper 15 parts, Radix Ginseng
Extract 15 parts, wolfberry fruit extract 6 parts.
Preferably, in step (1), pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, described pre-
Inorganic agent B is the reduced glutathion aqueous solution of mass fraction 1.0%.
Preferably, temperature 35-65 DEG C of step (2) mesohigh homogenizing, pressure 120-400Mpa, circulates homogenizing at least two
Secondary.
Preferably, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is the 0.2-of fresh Intestinum Stichopi japonici quality
0.8%.
Preferably, in described step (4), the enzyme activity of compound protease and neutral protease is than for 2-3:1, and enzyme is total
Consumption is the 0.6-1.0% of fresh Intestinum Stichopi japonici quality.
A kind of preparation method of the polypeptide capsule preventing copper deficiency disease, described preparation method is carried out as follows:
The first step: prepare Intestinum Stichopi japonici extract,
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, pretreating agent A
For the water-insoluble glucan suspension of mass fraction 8-12%, described pretreating agent B is the reduction of mass fraction 0.5-1.5%
Type glutathion aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is
1:2-3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or
Pretreating agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, under room temperature at stirring
Reason 2-2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds
Enter appropriate food-grade lipase, stir, enzymolysis 0.5-1h, Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3-
5min, is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, described NF membrane retain molecule
Amount is 150-300Da；
Second step, chelating: under conditions of 50-60 DEG C, add appropriate water-soluble copper in nanofiltration concentrated solution while stirring
Salt, the most slowly regulation system pH is 5.5-6.5, and the response time is 2～3h, maintenance system pH stable in course of reaction, instead
Should cool down after terminating, filter, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated copper；
3rd step, prepares capsule finished product,
Intestinum Stichopi japonici extract chelated copper, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, so
The polypeptide capsule of prevention copper deficiency disease is prepared by sterilizing, fill.
The invention has the beneficial effects as follows: the polypeptide capsule rich in nutrition content of prevention copper deficiency disease prepared by the present invention, easily
In absorbing, absorption rate is high, and impurity and content of beary metal are low, are of high nutritive value, both can be the most permissible with supplementation with copper element
Supplement the multiple nutrient coming from Intestinum Stichopi japonici.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail, inorganic in embodiment
Copper selects curpic carbonate.
Embodiment 1:
The polypeptide capsule of prevention copper deficiency disease, described capsule is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extracts
Thing chelated copper 10 parts, Radix Ginseng extract 10 parts, wolfberry fruit extract 5 parts, the preparation method of polypeptide capsule comprises the steps:
The first step, prepares Intestinum Stichopi japonici extract, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent
A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 8%, and described pretreating agent B is matter
The reduced glutathion aqueous solution of amount mark 0.5%, concrete pretreatment operation is: first use pretreating agent A pre-under room temperature
Processing 2h, solid-liquid ratio is 1:2, then stirs pretreatment 2h under room temperature with pretreating agent B, then stands 45minh, and solid-liquid ratio is
1:1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, high pressure homogenize temperature 35-45 DEG C, pressure 120Mpa, circulation homogenizing 3 twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35 DEG C, adjusts pH value to 6.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 3min, then cools down
Standing 1h to room temperature, remove supernatant oil layer, the enzyme work of described food-grade lipase is 20,000 U/g, and enzyme dosage is new fresh sea cucumber
The 0.2% of intestinal quality；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40 DEG C, adjusts pH value 6.5, adds appropriate
Complex food level protease hydrolyzed 2h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 10min, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant, composite flavor albumen
The enzyme activity of enzyme and neutral protease is than for 2:1, and the total consumption of enzyme is the 0.6% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-2000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.25 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-200Da；
Second step, chelating: under conditions of 50 DEG C, in nanofiltration concentrated solution, add moderate amount of sulfuric acid copper, simultaneously while stirring
Slowly regulation system pH is 5.5, and the response time is 2h, maintenance system pH stable in course of reaction, and reaction cools down after terminating, mistake
Filter, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated copper；
3rd step, prepares capsule finished product,
Intestinum Stichopi japonici extract chelated copper, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, so
The polypeptide capsule of prevention copper deficiency disease is prepared by sterilizing, fill.
Embodiment 2
The polypeptide capsule of prevention copper deficiency disease, described capsule is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extracts
Thing chelated copper 20 parts, Radix Ginseng extract 20 parts, wolfberry fruit extract 10 parts, the preparation method of polypeptide capsule comprises the steps:
Described capsule is prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent
A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 12%, and described pretreating agent B is
The reduced glutathion aqueous solution of mass fraction 1.5%, concrete pretreatment operation is: first use pretreating agent A under room temperature
Pretreatment 1h, solid-liquid ratio is 1:3, then stirs pretreatment 3h under room temperature with pretreating agent B, then stands 45min, and solid-liquid ratio is
1:1；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 60-65 DEG C of high pressure homogenize, pressure 400Mpa, circulation homogenizing twice；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 45 DEG C, adjusts pH value to 7.5, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.8% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds
Entering appropriate complex food level protease hydrolyzed 2-3h, described complex food level protease is by compound protease and neutral egg
White enzyme composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；Multiple
Closing the enzyme activity of flavor protease and neutral protease ratio for 3:1, the total consumption of enzyme is the 1.0% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000-8000Da is obtained
Ultrafiltration permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
200-300Da；
Second step, chelating: under conditions of 60 DEG C, add appropriate copper chloride, simultaneously in nanofiltration concentrated solution while stirring
Slowly regulation system pH is 6.5, and the response time is 3h, maintenance system pH stable in course of reaction, and reaction cools down after terminating, mistake
Filter, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated copper；
3rd step, prepares capsule finished product,
Intestinum Stichopi japonici extract chelated copper, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, so
The polypeptide capsule of prevention copper deficiency disease is prepared by sterilizing, fill.
Embodiment 3:
The polypeptide capsule of prevention copper deficiency disease, described capsule is formed by the Raw material processing of following weight portion: Intestinum Stichopi japonici extracts
Thing chelated copper 15 parts, Radix Ginseng extract 15 parts, wolfberry fruit extract 6 parts, the preparation method of polypeptide capsule comprises the steps:
Described capsule is prepared as follows:
The first step, prepares Intestinum Stichopi japonici extract, (1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent
A and B carries out pretreatment, and pretreating agent A is the water-insoluble glucan suspension of mass fraction 10%, and described pretreating agent B is
The reduced glutathion aqueous solution of mass fraction 1.0%, concrete pretreatment operation is: by pretreating agent A and pretreating agent B with
Volume ratio 1:1 mix homogeneously, is subsequently adding fresh Intestinum Stichopi japonici, and then stir process 2.5h under room temperature stands 1h, solid-liquid ratio
1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate
Water, carries out homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid, temperature 40-45 DEG C of high pressure homogenize, pressure 180Mpa, circulation homogenizing three times；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 40 DEG C, adjusts pH value to 7.0, adds appropriate
Food-grade lipase, stirs, enzymolysis 1h, and Intestinum Stichopi japonici is carried out defat, then heats to 75 DEG C of inactivation 5min, then cools down
Stand 2h to room temperature, remove supernatant oil layer；The enzyme work of food-grade lipase is 20,000 U/g, and enzyme dosage is fresh Intestinum Stichopi japonici matter
The 0.5% of amount；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 45 DEG C, adjusts pH value 6.8, adds appropriate
Complex food level protease hydrolyzed 2.5h, described complex food level protease is by compound protease and neutral protease group
Become, enzymolysis post-heating to 80 DEG C enzyme denaturing 12min, be then centrifuged for separating, obtain Intestinum Stichopi japonici secondary enzymolysis supernatant；Composite flavor albumen
The enzyme activity of enzyme and neutral protease is than for 2.5:1, and the total consumption of enzyme is the 0.75% of fresh Intestinum Stichopi japonici quality；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 6000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates,
The dialysis of ultrafiltration permeate be concentrated into dilution 0.3 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
220Da；
Second step, chelating: under conditions of 55 DEG C, in nanofiltration concentrated solution, add moderate amount of sulfuric acid copper, simultaneously while stirring
Slowly regulation system pH is 6.0, and the response time is 2.5h, maintenance system pH stable in course of reaction, reaction cools down after terminating,
Filtering, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated copper；
3rd step, prepares capsule finished product,
Intestinum Stichopi japonici extract chelated copper, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, so
The polypeptide capsule of prevention copper deficiency disease is prepared by sterilizing, fill.
It is organic that the polypeptide capsule of prevention copper deficiency disease prepared by the present invention includes that copper and Intestinum Stichopi japonici self contain
Element, rich in nutrition content, rich in several amino acids, small-molecular peptides, it is of high nutritive value, it is easy to absorb, absorption rate
Height, impurity content is low, and heavy metal does not detects, and mends copper prevention copper deficiency disease effect notable.
Embodiment described above is the one preferably scheme of the present invention, not makees the present invention any pro forma
Limit, on the premise of without departing from the technical scheme described in claim, also have other variant and remodeling.
Claims (8)
1. prevent the polypeptide capsule of copper deficiency disease, it is characterised in that: the implant of described polypeptide capsule is former by following weight portion
Material processes: Intestinum Stichopi japonici extract chelated copper 10-20 part, Radix Ginseng extract 10-20 part, wolfberry fruit extract 5-10 part, Stichopus japonicus
The preparation method of intestinal extract chelated copper comprises the steps:
The first step: prepare Intestinum Stichopi japonici extract；
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
Second step, chelating: under conditions of 50-60 DEG C, in nanofiltration concentrated solution, add suitable quantity of water dissolubility mantoquita while stirring, with
Time slow regulation system pH be 5.5-6.5, the response time is 2～3h, maintenance system pH stable in course of reaction, and reaction terminates
Rear cooling, filtration, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated copper.
The polypeptide capsule of prevention copper deficiency disease the most according to claim 1, it is characterised in that: described mantoquita is copper sulfate
And/or copper chloride.
Prevent the polypeptide capsule of copper deficiency disease the most according to claim 1, it is characterised in that: described polypeptide capsule is by following heavy
The Raw material processing of amount part forms: Intestinum Stichopi japonici extract chelated copper 15 parts, Radix Ginseng extract 15 parts, wolfberry fruit extract 6 parts.
The polypeptide capsule of prevention copper deficiency disease the most according to claim 1, it is characterised in that: pretreating agent A in step (1)
For the water-insoluble glucan suspension of mass fraction 10%, described pretreating agent B is the reduced form paddy Guang of mass fraction 1.0%
Sweet peptide aqueous solution.
The polypeptide capsule of prevention copper deficiency disease the most according to claim 1, it is characterised in that: step (2) mesohigh homogenizing
Temperature 35-65 DEG C, pressure 120-400Mpa, circulation homogenizing at least twice.
The polypeptide capsule of prevention copper deficiency disease the most according to claim 1, it is characterised in that: described food-grade lipase
Enzyme work is 20,000 U/g, and enzyme dosage is the 0.4-0.8% of fresh Intestinum Stichopi japonici quality.
The polypeptide capsule of prevention copper deficiency disease the most according to claim 1, it is characterised in that: compound in described step (4)
The enzyme activity of flavor protease and neutral protease is than for 2-3:1, and the total consumption of enzyme is the 0.6-1.0% of fresh Intestinum Stichopi japonici quality.
8. described in a claim 1-7 any one, prevent the preparation method of the polypeptide capsule of copper deficiency disease, it is characterised in that
Described preparation method is carried out as follows:
The first step: prepare Intestinum Stichopi japonici extract；
(1) Intestinum Stichopi japonici pretreatment: collect fresh Intestinum Stichopi japonici, uses pretreating agent A and B to carry out pretreatment, and pretreating agent A is matter
The water-insoluble glucan suspension of amount mark 8-12%, described pretreating agent B is the reduced form paddy of mass fraction 0.5-1.5%
Guang sweet peptide aqueous solution, concrete pretreatment operation is: first using pretreating agent A pretreatment 1-2h under room temperature, solid-liquid ratio is 1:2-
3, then under room temperature, stir pretreatment 2-3h with pretreating agent B, then standing 45min-1h, solid-liquid ratio is 1:1-2, or will be pre-
Inorganic agent A is mixed homogeneously with volume ratio 1:1 with pretreating agent B, is subsequently adding fresh Intestinum Stichopi japonici, stir process 2-under room temperature
2.5h, then stands 0.5-1h, and solid-liquid ratio 1:2, after pretreatment, solid-liquid separation is standby；
(2) homogenizing of Intestinum Stichopi japonici: step (1) pretreated Intestinum Stichopi japonici is placed in high pressure homogenizer, adds appropriate water, enter
Row homogenizing, obtains Intestinum Stichopi japonici homogenizing fluid；
(3) primary enzymolysis: step (2) gained Intestinum Stichopi japonici homogenizing fluid is warming up to 35-45 DEG C, adjusts pH value to 6.5-7.5, adds suitable
Amount food-grade lipase, stirs, and enzymolysis 0.5-1h carries out defat to Intestinum Stichopi japonici, then heats to 75 DEG C of inactivation 3-5min,
It is subsequently cooled to room temperature and stands 1-2h, remove supernatant oil layer；
(4) secondary enzymolysis: the primary enzymolysis liquid after step (3) being processed is warming up to 40-50 DEG C, adjusts pH value 6.5-7.0, adds suitable
The complex food level protease hydrolyzed 2-3h of amount, described complex food level protease is by compound protease and neutral protease
Composition, enzymolysis post-heating to 80 DEG C enzyme denaturing 10-15min, it is then centrifuged for separating, obtains Intestinum Stichopi japonici secondary enzymolysis supernatant；
(5) ultrafiltration: the ultrafilter membrane ultrafiltration that Intestinum Stichopi japonici secondary enzymolysis supernatant molecular cut off is 5000-8000Da is obtained ultrafiltration
Permeate；
(6) nanofiltration: diluted with appropriate pure water by the ultrafiltration permeate of step (6), then carries out dialysis by NF membrane and concentrates, ultrafiltration
Permeate dialysis be concentrated into dilution 0.25-0.35 times of front volume nanofiltration concentrated solution, the molecular cut off of described NF membrane is
150-300Da；
Second step, chelating: under conditions of 50-60 DEG C, in nanofiltration concentrated solution, add suitable quantity of water dissolubility mantoquita while stirring, with
Time slow regulation system pH be 5.5-6.5, the response time is 2～3h, maintenance system pH stable in course of reaction, and reaction terminates
Rear cooling, filtration, filtered solution is through being concentrated in vacuo and be spray-dried prepared Intestinum Stichopi japonici extract chelated copper；
3rd step, prepares capsule finished product,
Intestinum Stichopi japonici extract chelated copper, Radix Ginseng extract and the wolfberry fruit extract of formula ratio are mixed in proportion, then warp
Sterilizing, fill prepare the polypeptide capsule of prevention copper deficiency disease.
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