CN106260725A - A kind of method improving egg protein and feedstuff - Google Patents

A kind of method improving egg protein and feedstuff Download PDF

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Publication number
CN106260725A
CN106260725A CN201610803224.5A CN201610803224A CN106260725A CN 106260725 A CN106260725 A CN 106260725A CN 201610803224 A CN201610803224 A CN 201610803224A CN 106260725 A CN106260725 A CN 106260725A
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CN
China
Prior art keywords
laying hen
egg protein
zine oxide
nano zine
method improving
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Pending
Application number
CN201610803224.5A
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Chinese (zh)
Inventor
赵勇
闵令江
李兰
沈伟
张宏福
葛伟
张鹏飞
刘欣琪
张卫东
郝亚男
赖方农
王俊杰
刘京才
初美强
于帅
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Qingdao Agricultural University
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Qingdao Agricultural University
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Priority to CN201610803224.5A priority Critical patent/CN106260725A/en
Publication of CN106260725A publication Critical patent/CN106260725A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02Breeding vertebrates
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)

Abstract

The present invention relates to poultry field of reproduction, specifically disclose a kind of method improving egg protein.Present invention firstly discovers that the nano zine oxide enabling laying hen that daily dose 0.1 10.0mg/kg is administered orally significantly increases the content of egg protein, increase the content of some protein relevant to fetal development, be conducive to Embryo Gallus domesticus to grow, and these are also beneficial to the health of the mankind.Convenient on-demand interpolation can not only be accomplished by the method adding nano zine oxide in chicken feed, change dosage in time, and be capable of using on a large scale under auto-feeding system.The method of the invention is applied to poultry cultivation, the use safety of eggs can be ensure that, also promote the development of aquaculture simultaneously.

Description

A kind of method improving egg protein and feedstuff
Technical field
The present invention relates to nano material utilize and animals' reproduction field, specifically, relate to one and improve egg protein Method.
Background technology
In raising egg quality particularly egg, the content of protein is one of target of breeding layer chicken industry pursuit, also carries The important channel of high China laying hen economic benefit.China is genuine egg big producing country.China's egg yield in 2014 Reach 24,500,000 tons, be only second to Carnis Sus domestica yield (56,710,000 tons).Egg yield be about 4.5 times of U.S.'s yield, the 7 of India's yield About times, account for the 40% of world's egg total output.Egg is the outstanding person in wholefood, and the protein in egg is at egg In have very important status.Egg almost contains the complete nutrients matter of needed by human body: protein, lipid, inorganic salt, dimension Raw element etc., and contained nutritional labeling is the most digested.Egg is not only excellent source of nutrition, its price also phase To cheap.Egg protein is not only the food source of high-quality, grows again the nutrient substance providing required for Embryo Gallus domesticus.But due to By environment, nutrition, heredity, factor stress be waited to be affected, in egg, protein content Differences is relatively big, thus have impact on Egg is as the value of food, the incubation rate of chicken and the quality hatching chicken.And, egg is as a kind of function in recent years Property food research and development.Researcher is seeking the protein that utilizes egg to produce specific function, and main path is Utilize the way of transgenic, but transgenic has a series of potential safety hazard.Therefore, one is sought simple, safe and effective Improve method of protein in egg and become particularly important.
Nano zine oxide is a kind of Multifunction inorganic material, and particle diameter is 1~100nm, has skin effect, quantum chi Very little effect, macroscopic view tunnel-effect and coulomb blockade effect.Nano zine oxide is with its excellent characteristic, at cosmetics, clothing, food The industries such as product are fresh-keeping, medical science, electronics and rubber are extensively applied.Zinc is many in participating in animal body as animal body essential trace element Plant the metabolic activity of nutrient substance, promote growth promoter and the immunity of organism of animal.Zinc plays non-in female repro ductive system The most important effect.The birth of nano zine oxide, provides new approaches for finding new zinc source.Add as new zinc source feedstuff Agent, nano zine oxide also plays an important role at poultry industry.The absorption rate of nano zine oxide is better than to have scholar's research to find Traditional zinc source such as zinc sulfate and zinc oxide, can significantly improve piglet, chickling production performance.
Summary of the invention
For improving egg quality, increasing the kind of protein in egg, spy of the present invention provides one to improve egg protein Method.
The technical scheme that the inventive method is taked is as follows:
A kind of method improving egg protein, particularly as follows: make laying hen pass through oral way picked-up nano zine oxide, picked-up The weight ratio of daily dose and laying hen be 0.1-10.0mg/kg.
Further, the daily dose of laying hen picked-up nano zine oxide and the preferred 0.5-7.5mg/kg of weight ratio of laying hen, more Preferably 2.5mg/kg.
Further, described nano granular of zinc oxide is shaped as almost spherical.
Further, the particle diameter of described nano zine oxide is 1-50nm.
Further, the particle diameter of described nano zine oxide is 25-50nm, mean diameter 30nm.Empirical tests, particle diameter is excessive Assimilation effect is not good enough, and limited to the improvement effect of egg protein quality, particle diameter is the least, can cause high cost.
Further, the oral opportunity of described nano zine oxide is the laying hen 1.5-18 monthly age.The present invention is at laying hen 1.5 monthly age Time start to feed nano zine oxide, though now not starting to lay eggs, but laying hen Development of Reproductive System very important period, Stage i.e. starts the growth feeding nano zine oxide to regulate its reproductive system the most in early days.
Further, described nano zine oxide is absorbed by laying hen by addition in animal feed.
Further, described laying hen is during feeding, and feedstuff is replaced every day, to prevent feed spoilage.
It is a further object to provide a kind of feedstuff that can improve egg protein, the technical scheme of employing is such as Under:
A kind of feedstuff improving egg protein, containing the nano zine oxide of 1.0-40.0mg/kg (feedstuff weight).
Further, possibly together with Semen Maydis, miscellaneous grain crops, wheat bran, soybean cake, cottonseed cake, rapeseed cake, fishbone powder, phosphorus in described feedstuff One or more in acid hydrogen calcium, Sal, Oleum Glycines, vitamin and trace element premix complex, the consumption ability of said components Field technique personnel can select according to conventional amount used.
Relative to prior art, the beneficial effects of the present invention is:
(1) present invention enables laying hen that suitable dose nano zine oxide is administered orally and significantly increases the content of egg protein, Increase the kind of some protein relevant to fetal development, beneficially Embryo Gallus domesticus to grow, the incubation rate of chicken can be improved and incubate Dissolve the quality of chicken, and these protein are also beneficial to the health of the mankind, safely and effectively.By adding in animal feed The mode of nano zine oxide makes animal consumption nano zine oxide, can not only accomplish the most on-demand interpolation, change dosage in time, And can realize using on a large scale under auto-feeding system.
(2) nano zine oxide that the present invention uses can buied on the market, is applied to improve egg protein aspect, Considerable economic benefit and good social benefit can be brought.Application the method can allow breeding layer chicken field and cultivation self-employed entrepreneurs Directly be benefited, improve egg quality, improve plant and the income of cultivation self-employed entrepreneurs;Improve egg quality, it is ensured that people simultaneously The people are physically and mentally healthy, also promote the development of aquaculture simultaneously.
(3) according to China's egg annual production at 25,000,000 tons, if the egg price of high-quality per ton exceeds 300-500 unit RMB, direct economic benefit can improve 75-125 hundred million yuan, and overall economic benefit is considerable.
Accompanying drawing explanation
The body weight of 30 week old laying hens during Fig. 1 oral different daily dose nano zine oxide;
The egg production of 30 week old laying hens during Fig. 2 oral different daily dose nano zine oxide;
During Fig. 3 oral different daily dose nano zine oxide, the total protein content of 30 the produced eggs of week old laying hen (accounts for egg size Ratio);
Kinds of protein (the protein high pass that during Fig. 4 difference group laying hen 30 week old, in egg, single protein content increases Amount quantitative data).
Detailed description of the invention
Below in conjunction with embodiment, the preferred embodiment of the present invention is described in detail.It will be appreciated that following reality Execute providing merely to play descriptive purpose of example, be not used to the scope of the present invention is limited.The skill of this area Art personnel, in the case of without departing substantially from spirit of the invention and spirit, can carry out various amendment and replacement to the present invention.
Experimental technique used in following embodiment if no special instructions, is conventional method.
The reagent and the material that use in following example are commercial goods.The nano zine oxide used is purchased from Sigma company (St.Louis, MO, USA), for almost spherical, mean diameter is 30nm (most in the range of 25-50nm).
(1) selection of laying hen:
Selecting the sea blue brown laying hen of 960 6 week old, sea blue brown laying hen is purchased from Shandong Han Shi Zhong Ji factory, is divided at random by laying hen It it is 8 groups (often 120 chickens of group) nano zine oxide in order to various dose of throwing something and feeding.Often group sets 3 repetitions, 40 chickens of each repetition, Laying hen can free choice feeding and drinking-water.
(2) rearing conditions of laying hen:
Illumination 12h/d, indoor temperature is 21 ± 1 DEG C.By 960 laying hens after the adaptability raising of a week, from the 6th week Starting to add nano zine oxide age in feedstuff, the daily dose making 8 groups of laying hens take nano zine oxide is respectively 0mg/kg (comparison Group), 0.1mg/kg, 0.5mg/kg, 1.0mg/kg, 2.5mg/kg, 5.0mg/kg, 7.5mg/kg, 10.0mg/kg.Every day is changed Feedstuff.
(3) interpretation of result:
Collect egg respectively in 30 week old, laying hen body weight and egg production are measured, and use proteomics high pass The protein content of amount quantitative approach detection egg and composition, and protein functional assays.
The data result of above-mentioned analysis is processed: use SPASS20.0 statistical software that data are carried out single factor test side Difference analysis (ANOVA), P < 0.05 thinks that data are that there were significant differences, and P < 0.01 thinks that data difference heteropole is notable.Average is many Anharmonic ratio relatively uses DuncanShi to carry out, result Means ± SEM.
Shown in Fig. 1 for body weight during difference group laying hen 30 week old, visible in figure, between different groups, laying hen body weight is without significantly Difference, it can thus be appreciated that throwing something and feeding of nano zine oxide does not produce impact to laying hen body weight.
Shown in Fig. 2 for egg production during difference group laying hen 30 week old, visible in figure, average every laying hen between different groups Egg production is without significant difference, it can thus be appreciated that throwing something and feeding of nano zine oxide does not produce impact to laying hen egg yield.
Shown in Fig. 3 for egg total protein content during difference group laying hen 30 week old, visible in figure, nano zine oxide day Dosage egg total protein content when 0.5mg/kg, 1.0mg/kg, 2.5mg/kg, 5.0mg/kg, 7.5mg/kg is significantly higher than Matched group, as daily dose 2.5mg/kg, effect is optimum.Visible, when nano zine oxide daily dose is at 0.5-7.5mg/kg, can It is effectively increased the content of egg gross protein.
Kinds of protein (the egg increased for single protein content in egg during difference group laying hen 30 week old shown in Fig. 4 White matter high-throughput quantification data), visible in figure, nano zine oxide daily dose 0.1mg/kg, 0.5mg/kg, 1.0mg/kg, The kind that when 2.5mg/kg, 5.0mg/kg, 7.5mg/kg, 10.0mg/kg, in egg, single protein content increases is significantly more than Matched group, as daily dose 2.5mg/kg, effect is optimum.Visible, when nano zine oxide daily dose is at 0.1-10.0mg/kg, chicken The kinds of protein that in egg, single protein content increases increases (protein high-throughput quantification result).
The New raxa protein increased is carried out functional analysis, finds that the protein increased is mainly relevant with lysosome Protein (protection chicken infecting from antibacterial etc. in hatching process) and the albumen that saccharide/lipid/protein metabolism is relevant Matter (ensures the demand of energy in chicken hatching), Embryo Gallus domesticus can be conducive to grow, improve the incubation rate of chicken and hatch chicken Quality.
In summary, feed the nano zine oxide that daily dose is 0.5-7.5mg/kg and can improve albumen on certain level The content of matter and kind, especially when daily dose is 2.5mg/kg, effect is best.Therefore the method for the present invention is for improving egg egg The production practices of white matter provide a kind of beneficial reference.
Although the present invention is described in detail the most with a general description of the specific embodiments in literary composition, but at this On the basis of bright, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, exist Without departing from these modifications or improvements on the basis of spirit of the present invention, belong to the scope of protection of present invention.

Claims (10)

1. the method improving egg protein, it is characterised in that particularly as follows: make laying hen absorb nano oxygen by oral way Changing zinc, the daily dose of picked-up and the weight ratio of laying hen are 0.1-10.0mg/kg.
The method improving egg protein the most according to claim 1, it is characterised in that the particle diameter of described nano zine oxide For 1-50nm.
The method improving egg protein the most according to claim 1 and 2, it is characterised in that described nano zine oxide Oral opportunity is the laying hen 1.5-18 monthly age.
The method improving egg protein the most according to claim 2, it is characterised in that laying hen picked-up nano zine oxide Daily dose is 0.5-7.5mg/kg with the weight ratio of laying hen.
The method improving egg protein the most according to claim 2, it is characterised in that laying hen picked-up nano zine oxide Daily dose is 2.5mg/kg with the weight ratio of laying hen.
The method improving egg protein the most according to claim 1 and 2, it is characterised in that described nano zine oxide Grain is shaped as almost spherical.
The method improving egg protein the most according to claim 1 and 2, it is characterised in that described nano zine oxide leads to Cross to add in animal feed and absorbed by laying hen.
The method improving egg protein the most according to claim 7, it is characterised in that described laying hen is in the process of nursing In, feedstuff is replaced every day.
9. the feedstuff improving egg protein, it is characterised in that contain the nano oxygen of 1.0-40.0mg in every kilogram of feed Change zinc.
The method improving egg protein the most according to claim 9, it is characterised in that possibly together with jade in described feedstuff Rice, miscellaneous grain crops, wheat bran, soybean cake, cottonseed cake, rapeseed cake, fishbone powder, calcium hydrogen phosphate, Sal, Oleum Glycines, vitamin and trace element are pre- One or more in mixed complex.
CN201610803224.5A 2016-09-06 2016-09-06 A kind of method improving egg protein and feedstuff Pending CN106260725A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101049127A (en) * 2007-05-09 2007-10-10 湖南农业大学 Health care type feedstuff capable of raising content of lysozyme in blood of livestock and poultry, and animal product
CN101218959A (en) * 2008-01-30 2008-07-16 北京华牧伟业科技有限公司 Application of nano-zinc oxide as animal feed additives
CN102415354A (en) * 2011-11-17 2012-04-18 昆明华曦牧业集团有限公司 Low-cost zinc-enriched egg production method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101049127A (en) * 2007-05-09 2007-10-10 湖南农业大学 Health care type feedstuff capable of raising content of lysozyme in blood of livestock and poultry, and animal product
CN101218959A (en) * 2008-01-30 2008-07-16 北京华牧伟业科技有限公司 Application of nano-zinc oxide as animal feed additives
CN102415354A (en) * 2011-11-17 2012-04-18 昆明华曦牧业集团有限公司 Low-cost zinc-enriched egg production method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王建辉等: "纳米氧化锌在动物营养中的应用前景", 《饲料广角》 *

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