CN106260500A - The method promoting papain activity - Google Patents
The method promoting papain activity Download PDFInfo
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- CN106260500A CN106260500A CN201610644825.6A CN201610644825A CN106260500A CN 106260500 A CN106260500 A CN 106260500A CN 201610644825 A CN201610644825 A CN 201610644825A CN 106260500 A CN106260500 A CN 106260500A
- Authority
- CN
- China
- Prior art keywords
- papain
- semen pisi
- pisi sativi
- sativi protein
- enzymolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000004365 Protease Substances 0.000 title claims abstract description 63
- 108090000526 Papain Proteins 0.000 title claims abstract description 59
- 229940055729 papain Drugs 0.000 title claims abstract description 59
- 235000019834 papain Nutrition 0.000 title claims abstract description 59
- 230000000694 effects Effects 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000001737 promoting effect Effects 0.000 title claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 40
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 38
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 38
- 229920001184 polypeptide Polymers 0.000 claims abstract description 29
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 29
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 29
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 229940088598 enzyme Drugs 0.000 claims abstract description 14
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 claims abstract description 12
- 150000002576 ketones Chemical class 0.000 claims abstract description 11
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 11
- 125000004203 4-hydroxyphenyl group Chemical group [H]OC1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims abstract description 8
- -1 hydroxyl radical free radical Chemical class 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 13
- 230000003064 anti-oxidating effect Effects 0.000 claims description 6
- 235000013361 beverage Nutrition 0.000 claims description 5
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 239000008055 phosphate buffer solution Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 abstract description 5
- 238000004108 freeze drying Methods 0.000 abstract description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 4
- 235000019419 proteases Nutrition 0.000 abstract description 4
- 230000003078 antioxidant effect Effects 0.000 abstract description 2
- 235000013376 functional food Nutrition 0.000 abstract description 2
- 150000003254 radicals Chemical class 0.000 abstract description 2
- 125000005805 dimethoxy phenyl group Chemical group 0.000 abstract 1
- XZXCBTBAADXWDD-UHFFFAOYSA-N 1-(2,4-dimethoxyphenyl)piperazine Chemical class COC1=CC(OC)=CC=C1N1CCNCC1 XZXCBTBAADXWDD-UHFFFAOYSA-N 0.000 description 8
- 235000006539 genistein Nutrition 0.000 description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 5
- 229940045109 genistein Drugs 0.000 description 5
- TZBJGXHYKVUXJN-UHFFFAOYSA-N genistein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC(O)=C2C1=O TZBJGXHYKVUXJN-UHFFFAOYSA-N 0.000 description 5
- ZCOLJUOHXJRHDI-CMWLGVBASA-N genistein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 ZCOLJUOHXJRHDI-CMWLGVBASA-N 0.000 description 5
- 230000003712 anti-aging effect Effects 0.000 description 4
- 229960004279 formaldehyde Drugs 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 3
- 235000019256 formaldehyde Nutrition 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 125000004204 2-methoxyphenyl group Chemical group [H]C1=C([H])C(*)=C(OC([H])([H])[H])C([H])=C1[H] 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 239000006057 Non-nutritive feed additive Substances 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to protease, be specifically related to a kind of method promoting papain activity.The method of promotion papain activity of the present invention, it is by enzyme accelerator 8 ((4 (2,4 Dimethoxyphenyls) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone add in Semen Pisi sativi protein powder together with papain, Semen Pisi sativi protein powder is carried out enzymolysis and prepares Semen Pisi sativi protein polypeptide powder.The papain accelerator of the present invention improves the activity of papain, shortens enzymolysis time, improves enzymolysis efficiency；Obtain Semen Pisi sativi protein polypeptide powder through lyophilization after Semen Pisi sativi protein powder enzymolysis, remove free radical in hgher efficiency；Compared to being not added with the Semen Pisi sativi protein polypeptide powder of papain accelerator, hydroxyl radical free radical clearance rate improves 5.3～18%, it is possible to for defying age, the exploitation of antioxidative functional food.
Description
Technical field
The present invention relates to protease, be specifically related to a kind of method promoting papain activity.
Background technology
Papain is a kind of Heat stability is good, the proteolytic enzyme of natural safe and sanitary, another name papayotin or
Carase, molecular weight is 23406, is made up of a kind of single chain polypeptide, has low specific feature, Cys25, His159 and Asp158
These three amino acid residue is present in the position, active center of papain.Can carry in the root of Fructus Caricae, stem, leaf and fruit
Take out papain.According to the regulation of " GB 2760-2011 national food safety standard food additive uses standard ", wood
Melon protease can be used as the processing aid of food industry, i.e. enzyme preparation.
The extracting method of papain has oven drying method, the tannin sedimentation method, ultrafiltration, flocculence, salting out method, supercritical ultrasonics technology
Deng, but there is reduction enzymatic activity in most extracting method, and the purity obtaining product is low, pollutes the problems such as environment.Although
The advantages such as papain has pH scope when using wide, Heat stability is good, but limited by extraction process.
Genistein is modal a kind of flavone compound in nature, itself have antioxidation, antitumor action,
Protective action, the raising effect such as immunity and anti-inflammation to blood vessel.8 ((4 (2,4 Dimethoxyphenyl) piperazines 1
Base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone are genisteins and 1-(2,4-dimethyl
Phenyl) a kind of genistein derivant that piperazine obtains through chemosynthesis is the modification to genistein.8 ((4 (2,4 2
Methoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone molecular formula are
C28H28N2O5, molecular weight 472.52, its structural formula is as follows:
At present, 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 are had no both at home and abroad
(4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone application report in terms of promoting papain activity.
Summary of the invention
It is an object of the invention to provide a kind of employing 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,
7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone, as enzyme accelerator, promote the side of papain activity
Method, not only shortens the enzymolysis time of Semen Pisi sativi protein, raising enzymolysis efficiency, and the Semen Pisi sativi protein polypeptide powder obtained, removes freely
Base is in hgher efficiency.
The method of promotion papain activity of the present invention, is by papain accelerator 8 ((4 (2,4
Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone and Fructus Chaenomeliss
Protease adds in Semen Pisi sativi protein powder together, Semen Pisi sativi protein powder is carried out enzymolysis and prepares Semen Pisi sativi protein polypeptide powder.
Wherein:
The addition of papain accelerator is the 2～4% of papain quality, and the addition of papain is
The 10～12% of Semen Pisi sativi protein powder quality.
The pH of enzymolysis is 6.3～6.5, and hydrolysis temperature is 60～65 DEG C, and enzymolysis time is 2～2.5h.
Carrying out enzyme denaturing after enzymolysis, enzyme-removal temperature is 95～100 DEG C, and the enzyme denaturing time is 5～10min.
Specifically comprise the following steps that
Semen Pisi sativi protein powder is dissolved in phosphate buffer solution, adds papain and papain accelerator, warp
Obtaining pea polypeptide liquid after enzymolysis, enzyme denaturing, cold filtration, pea polypeptide liquid is the most freeze-dried obtains Semen Pisi sativi protein polypeptide powder.
Semen Pisi sativi protein polypeptide powder is added in common beverages with the concentration of 15～17.2mg/mL, makes antioxidation, anti-ageing
Old functional drinks.
Papain accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3
(4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone, for the synthetics of genistein Yu 1-(2,4-3,5-dimethylphenyl) piperazine,
Concrete preparation process is as follows:
Addition genistein 1mmol in 50mL round-bottomed flask, dehydrated alcohol 25mL and 37% formalin 0.08mL,
It is heated to 55～60 DEG C, after reactant liquor clarification, adds 1-(2,4-3,5-dimethylphenyl) piperazine 0.19mL, 48h is stirred at room temperature, have solid
Body separates out, and filters, and solid is isolated and purified through recrystallization, obtains the enzyme accelerator of the present invention.
In the present invention, papain accelerator is mutually tied with a hydrogen bond with an arginine residues of papain
Closing, and be combined with two other arginine residues respectively with four π keys, this combination result in changing of papain conformation
Become, thus improve the activity of papain.
Compared with prior art, beneficial effects of the present invention is as follows:
(1) the papain accelerator of the present invention improves the activity of papain, and it is together with papain
Add in Semen Pisi sativi protein powder, there is synergism, shorten enzymolysis time, improve enzymolysis efficiency.
(2) obtain Semen Pisi sativi protein polypeptide powder through lyophilization after Semen Pisi sativi protein powder enzymolysis, remove free radical in hgher efficiency；Phase
Comparing in the Semen Pisi sativi protein polypeptide powder being not added with papain accelerator, hydroxyl radical free radical clearance rate improves 5.3～18%, energy
It is enough in defying age, the exploitation of antioxidative functional food.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1
Being dissolved in by Semen Pisi sativi protein powder in the phosphate buffer solution that pH value is 6.5, forming Semen Pisi sativi protein powder mass concentration is
The dispersion liquid of 7%, adds the papain accounting for Semen Pisi sativi protein powder quality 12%, is simultaneously introduced and accounts for papain quality 2%
Papain accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxyls
Base phenyl) 4H .alpha.-5:6-benzopyran 4 ketone, it is 6.5 at pH value, under the conditions of temperature is 65 DEG C, enzymolysis 2h is shaken in water-bath, and enzymolysis terminates
After, enzymolysis solution maintains 5min enzyme denaturing at 100 DEG C, is then cooled to room temperature, is filtrated to get pea polypeptide liquid.Survey with formol titration
Amount degree of hydrolysis, after finding to add papain accelerator, the enzymolysis degree of Semen Pisi sativi protein improves 33%, and enzymolysis time shortens
25%.
Pea polypeptide liquid is obtained pea polypeptide powder through vacuum lyophilization.Pea polypeptide powder is added with the concentration of 16mg/mL
Being added in common beverages, obtain the functional drinks with antioxidation, anti-aging effects, its hydroxyl radical free radical clearance rate reaches
65%, compared with not using papain accelerator, clearance rate improves 18%.
Embodiment 2
Being dissolved in by Semen Pisi sativi protein powder in the phosphate buffer solution that pH value is 6.3, forming Semen Pisi sativi protein powder mass concentration is
The dispersion liquid of 7%, adds the papain accounting for Semen Pisi sativi protein powder quality 10%, is simultaneously introduced and accounts for papain quality 4%
Papain accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxyls
Base phenyl) 4H .alpha.-5:6-benzopyran 4 ketone, it is 6.3 at pH value, under the conditions of temperature is 60 DEG C, enzymolysis 2.5h is shaken in water-bath, and enzymolysis is tied
Shu Hou, enzymolysis solution maintains 10min enzyme denaturing at 95 DEG C, is then cooled to room temperature, is filtrated to get pea polypeptide liquid.Use formol titration
Measuring degree of hydrolysis, after finding to add papain accelerator, the enzymolysis degree of Semen Pisi sativi protein improves 16%, and enzymolysis time shortens
7%.
Pea polypeptide liquid is obtained pea polypeptide powder through vacuum lyophilization.Pea polypeptide powder is added with the concentration of 15mg/mL
Being added in common beverages, obtain the functional drinks with antioxidation, anti-aging effects, its hydroxyl radical free radical clearance rate reaches
58%, compared with not using papain accelerator, clearance rate improves 5.3%.
Embodiment 3
Being dissolved in by Semen Pisi sativi protein powder in the phosphate buffer solution that pH value is 6.4, forming Semen Pisi sativi protein powder mass concentration is
The dispersion liquid of 7%, adds the papain accounting for Semen Pisi sativi protein powder quality 11%, is simultaneously introduced and accounts for papain quality 3%
Papain accelerator 8 ((4 (2,4 Dimethoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxyls
Base phenyl) 4H .alpha.-5:6-benzopyran 4 ketone, it is 6.4 at pH value, under the conditions of temperature is 63 DEG C, enzymolysis 2.3h is shaken in water-bath, and enzymolysis is tied
Shu Hou, enzymolysis solution maintains 10min enzyme denaturing at 95 DEG C, is then cooled to room temperature, is filtrated to get pea polypeptide liquid.Use formol titration
Measuring degree of hydrolysis, after finding to add papain accelerator, the enzymolysis degree of Semen Pisi sativi protein improves 25%, and enzymolysis time shortens
15%.
Pea polypeptide liquid is obtained pea polypeptide powder through vacuum lyophilization.By pea polypeptide powder with the concentration of 17.2mg/mL
Adding in common beverages, obtain the functional drinks with antioxidation, anti-aging effects, its hydroxyl radical free radical clearance rate reaches
61%, compared with not using papain accelerator, clearance rate improves 11%.
Claims (6)
1. the method promoting papain activity, it is characterised in that: by papain accelerator 8 ((4 (2,4 two
Methoxyphenyl) piperazine 1 base) methyl) 5,7 dihydroxy 3 (4 hydroxy phenyl) 4H .alpha.-5:6-benzopyran 4 ketone and Fructus Chaenomelis egg
White enzyme adds in Semen Pisi sativi protein powder together, Semen Pisi sativi protein powder is carried out enzymolysis and prepares Semen Pisi sativi protein polypeptide powder.
The method of promotion papain activity the most according to claim 1, it is characterised in that: papain accelerator
Addition is papain quality 2～4%, the addition of papain be Semen Pisi sativi protein powder quality 10～
12%.
The method of promotion papain activity the most according to claim 1, it is characterised in that: enzymolysis pH value be 6.3～
6.5, hydrolysis temperature is 60～65 DEG C, and enzymolysis time is 2～2.5h.
The method of promotion papain activity the most according to claim 3, it is characterised in that: go out after enzymolysis
Enzyme, enzyme-removal temperature is 95～100 DEG C, and the enzyme denaturing time is 5～10min.
The method of promotion papain activity the most according to claim 1, it is characterised in that: Semen Pisi sativi protein powder is dissolved
In phosphate buffer solution, add papain and papain accelerator, obtain after enzymolysis, enzyme denaturing, cold filtration
Pea polypeptide liquid, pea polypeptide liquid is the most freeze-dried obtains Semen Pisi sativi protein polypeptide powder.
The method of promotion papain activity the most according to claim 5, it is characterised in that: by Semen Pisi sativi protein polypeptide powder
Add in common beverages with the concentration of 15～17.2mg/mL, obtain the functional drinks of antioxidation, defying age.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610644825.6A CN106260500A (en) | 2016-08-08 | 2016-08-08 | The method promoting papain activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610644825.6A CN106260500A (en) | 2016-08-08 | 2016-08-08 | The method promoting papain activity |
Publications (1)
| Publication Number | Publication Date |
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| CN106260500A true CN106260500A (en) | 2017-01-04 |
Family
ID=57666373
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610644825.6A Pending CN106260500A (en) | 2016-08-08 | 2016-08-08 | The method promoting papain activity |
Country Status (1)
| Country | Link |
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| CN (1) | CN106260500A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331301A (en) * | 2021-06-16 | 2021-09-03 | 怀仁市金沙滩羔羊肉业股份有限公司 | Preparation of enzyme modified pea protein and application of enzyme modified pea protein in low-fat red sausage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709320A (en) * | 2009-11-27 | 2010-05-19 | 广州市味研生物工程科技有限公司 | Method for preparing champignon polypeptide powder |
| CN101948895A (en) * | 2010-08-13 | 2011-01-19 | 江苏大学 | New method for development and utilization of potato residue resource |
-
2016
- 2016-08-08 CN CN201610644825.6A patent/CN106260500A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709320A (en) * | 2009-11-27 | 2010-05-19 | 广州市味研生物工程科技有限公司 | Method for preparing champignon polypeptide powder |
| CN101948895A (en) * | 2010-08-13 | 2011-01-19 | 江苏大学 | New method for development and utilization of potato residue resource |
Non-Patent Citations (3)
| Title |
|---|
| 刘娜等: "染料木素衍生物的合成及其生物活性研究 ", 《食品与药品》 * |
| 刘娜等: "染料木素衍生物的合成及其生物活性研究", 《食品与药品》 * |
| 耿杰: "槲皮素、染料木素衍生物的合成及其生物活性研究", 《中国优秀硕士学位论文数据库 工程科技I辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331301A (en) * | 2021-06-16 | 2021-09-03 | 怀仁市金沙滩羔羊肉业股份有限公司 | Preparation of enzyme modified pea protein and application of enzyme modified pea protein in low-fat red sausage |
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Application publication date: 20170104 |