CN106259465A - A kind of viral powder being suitable for the propagation of lepidoptera pest Carrier - Google Patents
A kind of viral powder being suitable for the propagation of lepidoptera pest Carrier Download PDFInfo
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- CN106259465A CN106259465A CN201610651753.8A CN201610651753A CN106259465A CN 106259465 A CN106259465 A CN 106259465A CN 201610651753 A CN201610651753 A CN 201610651753A CN 106259465 A CN106259465 A CN 106259465A
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- powder
- carrier
- viral
- virus
- senpv
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention provides a kind of viral powder being suitable for the propagation of lepidoptera pest Carrier, be mainly composed of the SeNPV former powder of virus, carrier and anti-caking agent；Obtaining most suitable carrier by contrast test is Pulvis Talci 6100A, and through measuring and calculating, the concentration obtaining viral former powder is 1.3 × 1011PIB/g, this insect viruses powder is applicable to the insect sex-attracting virus infection device of the present inventor's invention, it is a kind of biological insecticides that only beet armyworm is had control action, to people, animal and various beneficial organism safety, non-target organism is not had toxicity, insect is not developed immunity to drugs, Field epidemic can be formed, long-term control pest population, to ecological environment security, pollution-free.
Description
Technical field
The present invention relates to insect viruses technical field, especially, relate to one and be suitable for lepidoptera pest Carrier biography
The viral powder broadcast.
Background technology
The lepidoptera pests such as beet armyworm (Spodoptera exigua H ü bner) are class primary pests on vegetable, all
Having fulminant, binge, (department rises cloud etc. 2009 to be easily generated the features such as Drug resistance；Liu Xiangyang etc. 2007；Moulton et al
2000), causing difficulty of prevention and cure to increase, cost accounting improves, and pesticide residues exceed standard, and directly threatens food safety, hinders externally
Trade Development, destroys ecological balance (Lu Kai etc. 2005；Huang answers family 2013).
Vegetable-crop pest-insect integrated control, in addition to chemical pesticide, also includes Biological control, such as laphygma exigua nuclear polyhedrosis virus
With sex pheromone production in beet armyworm, Spodoptera exigua, the two tradition using method is respectively nebulization and mass trapping, and virus nebulization effective object is
Vegetable-crop pest-insect larva, and have cost accounting high, the features such as prevention effect is not prominent, and sex pheromone mass trapping acts on vegetable evil
Worm adult, only has preventive and therapeutic effect to male imago, have ignored the male imago repeatedly mating ability that residue end lures, to Population Control
Limited in one's ability.
On the basis of proposing beet armyworm gyplure and the integrated Control Technology of virus, it is thus achieved that lure based on insects
" insect sex-attracting virus infection device " utility model patent of the integrated prevention and control of virus, is integrated with sex pheromone and nuclear polyhedrosis virus
Control prece, changes tradition prevention and controls, reduces cost accounting, particularly cost of labor, eliminate pesticide residues phenomenon, energy
Preferably solve a resistanc pest prevention and control difficult problem.In this control prece, the preparation formulation of virus is a kind of key factor, should
Ensure virus effective content in the formulation, so that insect carries the most lethal viral dosage, carry out field vertical transmission, also
Requirements such as must meeting that dosage form is water insoluble, nonhygroscopic, do not lump, be portable, the characteristic that protection virus simultaneously is degraded less in field.
Laphygma exigua nuclear polyhedrosis virus (Spodoptera exigua nucleopolyhedrovirus, SeNPV)
Being the important entomopathogen of a class, polyhedral body is irregular shape, and majority is pentagon or hexagon.Its pesticide toxicology
Test shows, rat acute per os and percutaneous toxicity all belong to low toxicity, has no stimulation eye and skin, hypersensitive is belonged to 0
The weak sensitizer of level, without therapeutic, is a kind of biological insecticides only having control action to beet armyworm.This insecticide has biology
All advantages of pesticide, i.e. to people, animal and various beneficial organism safety, do not have toxicity to non-target organism, do not produce insect
Drug resistance, can form Field epidemic, long-term control pest population, to ecological environment security, pollution-free.
At present, powder formulation production used have for spraying wettable powder (WP), soluble powder (SP),
Water-soluble powder (AF), the powder (DP) used for dusting, dust agent (DPC), and the bait powder (BP) as bait formulation, tag
Powder of tagging (CP).It is soluble in water that above dosage form has, and being difficult to of having is sticked, carried, and all not being suitable for property lure and virus
In the application of integrated Control Technology, therefore it is contemplated that propose a kind of lepidoptera pest field that is suitable for carry the viral agent of propagation
Type.
Summary of the invention
The purpose of the present invention is that the Lepidoptera vegetable-crop pest-insect green bio Control Technology such as beet armyworm lure and virus
Integrated Control Technology provides a kind of special-purpose assorted virus dosage form, and this dosage form is water insoluble in field, nonhygroscopic, do not lump, flowing
Property good, it is easy to insect is sticked, carries and propagates, overcome existing dosage form this integrated technology use on deficiency, thus ensure should
Item technology reaches good prevention effect.
A kind of viral powder being suitable for the propagation of lepidoptera pest Carrier, including the composition of following weight ratio:
SeNPV 5 parts of former powder of virus
59 parts of carrier
Anti-caking agent 1 part.
Preferably, it is 1.3 × 10 that the described SeNPV former powder of virus obtains concentration11PIB/g。
Preferably, described carrier is Pulvis Talci 6100A.
The present invention, in the selection course to carrier, have chosen 6 kinds of carriers and tests, concrete test method and result
As follows:
With 6 kinds of carriers such as Pulvis Talci 6100A, Pulvis Talci 818, Pulvis Talci 934071, attapulgite, kieselguhr, Kaolin
In the environment of hot and humid (35 DEG C, 100%), place 3d, synthesis result analysis, draw being ordered as of anti-caking performance: Talcum
Powder 6100A ＞ Pulvis Talci 818 ＞ Pulvis Talci 934071 ＞ attapulgite ＞ kieselguhr ＞ Kaolin, Pulvis Talci 6100A caking is existing
As showing themselves in that carrier surface, substantially without balling phenomenon, does not bonds with culture dish wall, good fluidity, start test with carrier
Time difference little.Other carrier all shows the phenomenons such as caking in various degree, poor fluidity.Therefore Pulvis Talci 6100A is selected to do
Main carriers for this preparation.
6 kinds of carriers such as Pulvis Talci 6100A are carried out the test of the adhesive capacity to beet armyworm, thin from three kinds of differences of Pulvis Talci
Degree product is it can be seen that along with the increase of fineness, adhesive capacity is obviously improved.Relatively four kinds of fineness all can at the carrier of 1250 mesh
To find out, fineness is identical but carrier that composition is different, and its adhesive capacity is the most different.And Pulvis Talci 6100A average single head Radix Betae night
Moth male moth carrying amount is 2.523mg, and adhesion property is apparently higher than other 5 kinds of carriers.
Beet armyworm male moth is after SeNPV powder surface creep, and powder mainly sticks to its abdominal surface and afterbody
In cercus, during itself and female moth mating, SeNPV powder can pass to female moth.Tests determined every female moth carries powder
Quality is 0.48mg, and it is that single male moth carries the 18.98% of SeNPV amount that the most female single moth carries SeNPV amount.
Preferably property lures viral integrated Control Technology tailored version virus powder to do criterion with field holding effect for 15d,
So on the basis of ensureing the SeNPV least concentration needed for preventive effect, the degradation amount during its holding effect must be counted.Therefore
The maximum value calculation declined according to preventive effect, it is ensured that the SeNPV powder minimum content needed for preventive effect should improve 20%.
According to the SeNPV virulence test to beet armyworm newly hatched larvae, further determined that and incubate children at the beginning of preventing and treating beet armyworm
The conveniently content of worm is that average single head beet armyworm newly hatched larvae carries 5000PIB.At most laying eggs with beet armyworm simultaneously
Measure 300, calculate to pieces of an egg loss rate 60% with female moth band poison simultaneously, in special dosage form the content of virus be 0.977 ×
1010PIB/g, to ensure the prevention effect during its field holding effect.To sum up analyze, the most viral lure integrated prevention and control
The concentration of technology-specific type SeNPV powder is set as 1 × 1010, both compensate for the loss of degradation, the most suitable improves
SeNPV concentration, preferably ensure that the prevention effect to beet armyworm.
SeNPV is carried out live body propagation, the polypide obtained is ground, cross 1250 eye mesh screens, obtain SeNPV virus former
Through blood counting chamber method, powder, determines that concentration is 1.3 × 1011PIB/g.By 1.3 × 1011PIB/g SeNPV virus former powder, Talcum
Powder 6100A, anti-caking agent according to 5:59:1 ratio mix, be fully ground and stir, i.e. join 1 × 1010PIB/g tailored version
SeNPV powder, is the viral powder being suitable for the propagation of lepidoptera pest Carrier of the present invention.
Preferably, the viral powder of the present invention is applicable to insect sex-attracting virus infection device (ZL 2,010 20660450.0) invention
People: department rises cloud, department gets over, Zhou Lilin.
The method have the advantages that the present invention's is suitable for the viral powder that lepidoptera pest Carrier is propagated
Agent, is mainly composed of the SeNPV former powder of virus, carrier and anti-caking agent；Most suitable carrier talc is obtained by contrast test
6100A, and through measuring and calculating, the concentration obtaining viral former powder is 1.3 × 1011PIB/g, this insect viruses powder can apply to this
The insect sex-attracting virus infection device of inventor's invention, is a kind of biological insecticides only having control action to beet armyworm, to people, animal
And various beneficial organism safety, non-target organism is not had toxicity, insect is not developed immunity to drugs, epidemic diseases can be formed and control for a long time
Insect processed, to ecological environment security, pollution-free.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Detailed description of the invention
Hereinafter embodiments of the invention are described in detail, but the present invention can limit according to claim and cover
Multitude of different ways implement.
Embodiment 1
A kind of viral powder being suitable for the propagation of lepidoptera pest Carrier, including the composition of following weight ratio:
SeNPV 5 parts of former powder of virus
Pulvis Talci 6100A 59 parts
Anti-caking agent 1 part.
The viral effective ingredient concentration of the described SeNPV former powder of virus is 1.3 × 1011PIB/g。
The viral powder of the present invention is applied to insect sex-attracting virus infection device (ZL 2,010 20660450.0).
The viral powder of embodiment 1 is carried out the test of the adhesive capacity to beet armyworm, average single head beet armyworm male moth
Carrying amount is 2.523mg, and beet armyworm male moth is after SeNPV powder surface creep, and powder mainly sticks to its abdominal surface
With in the cercus of afterbody, during itself and female moth mating, SeNPV powder can pass to female moth.Tests determined every female moth
Carrying powder quality is 0.48mg, and it is that single male moth carries the 18.98% of SeNPV amount that the most female single moth carries SeNPV amount.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, that is made any repaiies
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (5)
1. one kind is suitable for the viral powder that lepidoptera pest Carrier is propagated, it is characterised in that include following weight ratio
Composition:
SeNPV 5 parts of former powder of virus
59 parts of carrier
Anti-caking agent 1 part.
It is suitable for the viral powder that lepidoptera pest Carrier is propagated the most as claimed in claim 1, it is characterised in that described
The SeNPV former powder of virus to obtain concentration be 1.3 × 1011PIB/g。
It is suitable for the viral powder that lepidoptera pest Carrier is propagated the most as claimed in claim 1, it is characterised in that described
Carrier be Pulvis Talci 6100A.
It is suitable for the viral powder that lepidoptera pest Carrier is propagated the most as claimed in claim 1, it is characterised in that described
Lepidoptera pest be beet armyworm.
It is suitable for the viral powder that lepidoptera pest Carrier is propagated the most as claimed in claim 1, it is characterised in that described
Viral powder be applied to insect sex-attracting virus infection device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610651753.8A CN106259465A (en) | 2016-08-10 | 2016-08-10 | A kind of viral powder being suitable for the propagation of lepidoptera pest Carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610651753.8A CN106259465A (en) | 2016-08-10 | 2016-08-10 | A kind of viral powder being suitable for the propagation of lepidoptera pest Carrier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106259465A true CN106259465A (en) | 2017-01-04 |
Family
ID=57667994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610651753.8A Pending CN106259465A (en) | 2016-08-10 | 2016-08-10 | A kind of viral powder being suitable for the propagation of lepidoptera pest Carrier |
Country Status (1)
| Country | Link |
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| CN (1) | CN106259465A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1346886A (en) * | 2001-09-07 | 2002-05-01 | 武汉普洛森生物制剂有限公司 | Spodoptera exigua Multi-nucleocapside Nucleopolyedrovirus and its bioinsecticide |
| CN201919530U (en) * | 2010-12-15 | 2011-08-10 | 司越 | Insect sex-attracting virus infection device |
-
2016
- 2016-08-10 CN CN201610651753.8A patent/CN106259465A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1346886A (en) * | 2001-09-07 | 2002-05-01 | 武汉普洛森生物制剂有限公司 | Spodoptera exigua Multi-nucleocapside Nucleopolyedrovirus and its bioinsecticide |
| CN201919530U (en) * | 2010-12-15 | 2011-08-10 | 司越 | Insect sex-attracting virus infection device |
Non-Patent Citations (4)
| Title |
|---|
| 丛晓平，: ""性引诱剂与病毒集成防控甜菜夜蛾技术研究"", 《中国优秀硕士学位论文全文数据库（电子期刊） 农业科技辑》 * |
| 刘琴 等，: ""甜菜夜蛾核型多角体病毒对甜菜夜蛾幼虫的毒力测定"", 《江苏农业科学》 * |
| 叶钟音主编，: "《现代农药应用技术全书》", 31 October 2002, 中国农业出版社 * |
| 汤历 等，: ""10亿PIB_mL甜菜夜蛾核型多角体病毒悬浮剂的配方研制与应用"", 《广东农业科学》 * |
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Application publication date: 20170104 |