CN106248957B - A kind of biochip for being used to detect human heat shock protein - Google Patents
A kind of biochip for being used to detect human heat shock protein Download PDFInfo
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- CN106248957B CN106248957B CN201610561638.1A CN201610561638A CN106248957B CN 106248957 B CN106248957 B CN 106248957B CN 201610561638 A CN201610561638 A CN 201610561638A CN 106248957 B CN106248957 B CN 106248957B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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- Urology & Nephrology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a kind of for detecting the biochip of human heat shock protein, including glass substrate, further include the detection plate set on a glass substrate, bottom surface and the upper surface of glass substrate fit below detection plate, the detection plate is equipped with detection liquid and adds in mouth and one group of detection mouth, detection liquid adds in mouth and is connected by channel with each detection mouth, is equipped on the outside of each detection mouth in the detection plate and places mouth for placing the liquid-absorbing block of liquid-absorbing block.The biochip is reasonable in design, and a variety of human heat shock proteins can be detected simultaneously with a biochip, save biochip, reduces testing cost, and detection efficiency is high.
Description
Technical field
The present invention relates to biochip technology field, more particularly, to a kind of biological core for being used to detect human heat shock protein
Piece.
Background technology
Human heat shock protein is related to various diseases, and human heat shock protein type is relatively more.Current human heat shock protein
Detection chip a certain human heat shock protein can only be detected, when detection need to prepare a variety of detection chips carry out it is independent
Detection, biochip function is single, of high cost, and detection efficiency is low.
Invention content
In view of the shortcomings of the prior art, the technical problems to be solved by the invention are to provide one kind for detecting human heat shock protein
White biochip can while be detected a variety of human heat shock proteins, the high purpose of detection efficiency with reaching.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
This is used for the biochip for detecting human heat shock protein, including glass substrate, further includes and sets on a glass substrate
Detection plate, bottom surface and the upper surface of glass substrate fit below detection plate, the detection plate be equipped with detection liquid add in mouth and
One group of detection mouth, detection liquid adds in mouth and is connected by channel with each detection mouth, in each detection mouth in the detection plate
Outside is equipped with places mouth for placing the liquid-absorbing block of liquid-absorbing block.
Further, the channel in the detection plate includes main channel and subchannel, and main channel is directly added in detection liquid
Mouth is connected, and each detection mouth is connected by subchannel with main channel.
The upper surface edge of the glass substrate is equipped with raised item, and the bottom surface edge of detection plate is equipped with corresponding with raised item
Positioning groove.
The housing for fixed test plate and glass substrate is further included, is set inwardly for opening, opening above the housing
There is the snap-gauge of protrusion, detection plate and glass substrate are located between the bottom plate of housing and snap-gauge.
The both sides of the main channel are equipped with subchannel, and the subchannel of main channel both sides shifts to install.
The positioning groove is dovetail groove.
Cover board is further included, the snap-gauge is equipped with card slot, and deck side edge can be inserted into card slot.
The bottom plate of the housing is equipped with through-hole.
The upper surface edge of the detection plate is equipped with positioning convex strip, and the snap-gauge is equipped with and the corresponding limit of positioning convex strip
Position slot.
The cover board is L-square, and L-square includes transverse slat and riser, and transverse slat is corresponding with the opening of upper part of the housing, riser
It is corresponding with the opening of housing side.
Compared with prior art, the present invention it has the following advantages：The biochip is reasonable in design, with a biological core
Piece can simultaneously be detected a variety of human heat shock proteins, save biochip, reduce testing cost, and detection efficiency is high.
Description of the drawings
The content to this specification expressed by each width attached drawing and the label in figure are briefly described below：
Fig. 1 is present invention detection plate structure schematic diagram.
Fig. 2 is glass substrate structure diagram of the present invention.
Fig. 3 is inventive shell structure diagram.
Fig. 4 is covering plate structure schematic diagram of the present invention.
In figure：1. detection plate, 101. detection liquid add in mouth, 102. main channels, 103. subchannels, 104. detection mouths, 105.
Liquid-absorbing block, 106. positioning convex strips, 2. glass substrates, 201. raised items, 202. convex blocks, 3. housings, 301. snap-gauges, 302. limitings
Slot, 303. card slots, 4. cover boards.
Specific embodiment
Below against attached drawing, by the description of the embodiment, making to the specific embodiment of the present invention further details of
Explanation.
As shown in Figures 1 to 4, biochip for being used to detect human heat shock protein, including housing 3, cover board 4, glass
Substrate 2 and detection plate 1, wherein, detection plate 1 is located in glass substrate 2, and glass substrate 2 is the support carrier of detection plate, is detected
Plate is located at the surface of glass substrate, and detection plate is as glass substrate size dimension, uniform rectangle tabular structure.
Bottom surface and the upper surface of glass substrate fit below detection plate, and the upper surface edge of glass substrate 2 is equipped with protrusion
Item 201, the bottom surface edge of detection plate be equipped with the corresponding positioning groove of raised item, positioning groove is dovetail groove；Glass substrate
Raised item one end on the inside of be equipped with convex block 202, positioning groove one end be equipped with slot corresponding with convex block, to detection plate and glass substrate
It is positioned.Detection plate is rubber slab, rubber slab and glass substrate flexible contact, and fitting effect is good.
Detection plate 1 adds in mouth 101 and one group of detection mouth 104 equipped with liquid is detected, and detection liquid addition mouth is by channel and respectively
A detection mouth is connected；Specifically, channel includes main channel 102 and subchannel 103, main channel 102 is directly added in detection liquid
Mouth 101 is connected, and each detection mouth 104 is connected by subchannel with main channel, is all provided on the outside of each detection mouth in detection plate
The liquid-absorbing block for being useful for placing liquid-absorbing block 105 places mouth, and liquid-absorbing block is the paper block or sponge block that can be absorbed water.It can by liquid-absorbing block
The detection liquid for adding in detection liquid addition mouth is drawn onto automatically in each detection mouth, various human heat shock proteins can be added in detection mouth
Antigen or antibody；The both sides of main channel are equipped with subchannel, and the subchannel of main channel both sides shifts to install, it is ensured that detection
Reliability.
Housing 3 is fixed the two for accommodating detection plate and glass substrate.Housing 3 be rectangular box-like structure, shell
3 top of body and one side are opening, and housing upper opening is equipped with the snap-gauge 301 of protrusion inwardly, and detection plate and glass substrate are set
Between the bottom plate and snap-gauge of housing.It can be inserted into housing by the opening of housing side after detection plate and glass substrate cooperation.
The upper surface edge of detection plate 1 is equipped with positioning convex strip 106, and snap-gauge 301 is equipped with and the corresponding limit of positioning convex strip
Position slot 302, detection plate and glass substrate are inserted into housing, ensure the position of the two in the housing by positioning convex strip, fixed
It is reliable and stable.
Snap-gauge 301 is equipped with card slot 303, and 4 side of cover board can be inserted into card slot.Cover board is L-square, and L-square includes horizontal stroke
Plate and riser, transverse slat is corresponding with the opening of upper part of the housing, and riser is corresponding with the opening of housing side.Cover board is hollow modeling
Flitch, cover board are squeezed elastically deformable, cover board be inserted into card slot position it is reliable.
The bottom plate of housing 3 is equipped with through-hole or housing is transparent housing, it is ensured that detection result；Such as the bottom plate of housing
Through-hole is equipped with, through-hole can be rectangular through-hole, pass through support of the edge of through-hole to glass substrate.
A variety of human heat shock proteins can be detected simultaneously with a biochip, save biochip, reduce detection
Cost, detection efficiency are high.
The present invention is exemplarily described above in conjunction with attached drawing, it is clear that present invention specific implementation is not by aforesaid way
Limitation, as long as employ the present invention design and technical solution carry out various unsubstantialities improvement or it is not improved will
The design and technical solution of the present invention directly applies to other occasions, within protection scope of the present invention.
Claims (9)
1. it is a kind of for detecting the biochip of human heat shock protein, including glass substrate, it is characterised in that：It further includes and is located at glass
The upper surface of detection plate on glass substrate, detection plate lower section bottom surface and glass substrate fits, and the detection plate is equipped with detection
Liquid adds in mouth and one group of detection mouth, and detection liquid adds in mouth and is connected by channel with each detection mouth, every in the detection plate
It is equipped on the outside of a detection mouth and places mouth for placing the liquid-absorbing block of liquid-absorbing block；It further includes for fixed test plate and glass base
The housing of piece, the housing top is opening, and opening is equipped with the snap-gauge protruded inwardly, and detection plate and glass substrate are located at housing
Bottom plate and snap-gauge between.
2. it is used to detect the biochip of human heat shock protein as described in claim 1, it is characterised in that：In the detection plate
Channel includes main channel and subchannel, and main channel directly adds in mouth with detection liquid and is connected, and each detection mouth passes through subchannel and master
Channel is connected.
3. it is used to detect the biochip of human heat shock protein as described in claim 1, it is characterised in that：The glass substrate
Upper surface edge is equipped with raised item, and the bottom surface edge of detection plate is equipped with and the corresponding positioning groove of raised item.
4. it is used to detect the biochip of human heat shock protein as claimed in claim 2, it is characterised in that：The two of the main channel
Side is equipped with subchannel, and the subchannel of main channel both sides shifts to install.
5. it is used to detect the biochip of human heat shock protein as claimed in claim 3, it is characterised in that：The positioning groove is
Dovetail groove.
6. it is used to detect the biochip of human heat shock protein as described in claim 1, it is characterised in that：Cover board is further included, institute
Snap-gauge is stated equipped with card slot, deck side edge can be inserted into card slot.
7. it is used to detect the biochip of human heat shock protein as described in claim 1, it is characterised in that：The bottom plate of the housing
It is equipped with through-hole.
8. it is used to detect the biochip of human heat shock protein as described in claim 1, it is characterised in that：The detection plate it is upper
Surface edge is equipped with positioning convex strip, and the snap-gauge is equipped with and the corresponding limiting slot of positioning convex strip.
9. it is used to detect the biochip of human heat shock protein as claimed in claim 6, it is characterised in that：The cover board is right angle
Plate, L-square include transverse slat and riser, and transverse slat is corresponding with the opening of upper part of the housing, and riser is opposite with the opening of housing side
It should.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610561638.1A CN106248957B (en) | 2016-07-15 | 2016-07-15 | A kind of biochip for being used to detect human heat shock protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610561638.1A CN106248957B (en) | 2016-07-15 | 2016-07-15 | A kind of biochip for being used to detect human heat shock protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106248957A CN106248957A (en) | 2016-12-21 |
| CN106248957B true CN106248957B (en) | 2018-07-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610561638.1A Active CN106248957B (en) | 2016-07-15 | 2016-07-15 | A kind of biochip for being used to detect human heat shock protein |
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| CN (1) | CN106248957B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2565022Y (en) * | 2002-08-23 | 2003-08-06 | 上海博昇微晶科技有限公司 | Microfluid chip with three-layer structure |
| KR20030092378A (en) * | 2002-05-29 | 2003-12-06 | 박영미 | Method and kit for diagnosis of progress stage of solid cancer |
| CN103713139A (en) * | 2013-12-14 | 2014-04-09 | 凌中鑫 | Test paper for human heat shock protein (Hsp 90alpha) and preparation method thereof |
| CN103760361A (en) * | 2013-12-14 | 2014-04-30 | 凌中鑫 | Biological chip for detecting person Hsp90a (heat shock protein 90) and detection method thereof |
-
2016
- 2016-07-15 CN CN201610561638.1A patent/CN106248957B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030092378A (en) * | 2002-05-29 | 2003-12-06 | 박영미 | Method and kit for diagnosis of progress stage of solid cancer |
| CN2565022Y (en) * | 2002-08-23 | 2003-08-06 | 上海博昇微晶科技有限公司 | Microfluid chip with three-layer structure |
| CN103713139A (en) * | 2013-12-14 | 2014-04-09 | 凌中鑫 | Test paper for human heat shock protein (Hsp 90alpha) and preparation method thereof |
| CN103760361A (en) * | 2013-12-14 | 2014-04-30 | 凌中鑫 | Biological chip for detecting person Hsp90a (heat shock protein 90) and detection method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106248957A (en) | 2016-12-21 |
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Effective date of registration: 20171127 Address after: 241000 Anhui Province, Wuhu city Jinghu District long Fang Culture Park (big long Fang No. 77) B03 Building Room 303 Applicant after: Anhui National Science Biotechnology Co., Ltd. Address before: West Road in Shushan District of Hefei City, Anhui Province, No. 32 230000 Applicant before: Tao Shaoqiang Applicant before: Li Yufeng Applicant before: Zhang Shiwei Applicant before: Zhang Bin Applicant before: Wang Xiaoyang Applicant before: Yang Yanqi |
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