CN106238023B - A kind of coating method for improving blood purification material surface biocompatibility - Google Patents
A kind of coating method for improving blood purification material surface biocompatibility Download PDFInfo
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- CN106238023B CN106238023B CN201610705333.3A CN201610705333A CN106238023B CN 106238023 B CN106238023 B CN 106238023B CN 201610705333 A CN201610705333 A CN 201610705333A CN 106238023 B CN106238023 B CN 106238023B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3268—Macromolecular compounds
- B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
- B01J20/3206—Organic carriers, supports or substrates
- B01J20/3208—Polymeric carriers, supports or substrates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
Abstract
The present invention relates to a kind of coating methods for improving blood purification material surface biocompatibility, include the following steps：S1, adsorbing agent carrier processing：According to the target substance of absorption, the pore-size distribution that selects is 3~30nm, particle diameter distribution is 0.3~1.2mm, specific surface area is 800~1200m20.6~0.9cm of/g, Kong Rongwei3The porous adsorbent of/g, by the medical grade purified treatment of series；S2, adsorbing agent carrier coating：Including following sub-step：S21, configuration sulfonated polysulfone coating liquid；S22, coating；S3, performance of the adsorbent evaluation：Evaluate its validity and safety.The advantage of the invention is that：It is coated with the sulfonated polysulfone with good blood compatibility in adsorbent surface, under the premise of not blocked the pore structure of suction-operated, the blood compatibility of adsorbent surface can effectively be improved, reduce absorption of the adsorbent to plasma protein, blood platelet, reduce the probability that the adverse reactions such as blood coagulation, haemolysis, complement activation occur, make product that there is good safety, and the good absorption property of adsorbent can be kept.
Description
Technical field
The present invention relates to a kind of coating methods for improving blood purification material surface biocompatibility.
Background technology
The research of Blood index treatment disease starts from the forties in last century.Blood is inhaled from the phase at the beginning of the eighties in last century in China
Attached dose conducts in-depth research, and is widely used, especially in drug poisoning, hepatic failure, kidney failure, autoimmunity
Disease, critical illness etc. achieve fast development.Rapid development is especially obtained between last decade, application range is increasingly
Extensively.What the Ministry of Public Health printed and distributed for 2 months 2010《Blood purification standard practice instructions》, the clear stipulaties adaptation of Blood index technology
Range is demonstrate,proved, it is saturating to can be widely applied to maintenance as a kind of critical treatment pattern in blood purification technology for Blood index technology
Analyse the multiple fields such as complication, severe liver diseases, acute poisoning, autoimmune disease, critical illness and hyperlipidemia.
Now applied to adsorbent mainly active charcoal and the synthetic resin of Blood index, it is modified without surface-coating
Activated carbon or synthetic resin are easy to cause blood coagulation, damage blood cells, particles from getting loose when some researches show that it with contacting blood
Lead to thrombus into blood, limits the application of activated carbon and synthetic resin in Blood index field.
Problem when in order to solve sorbent material for Blood index, scientists coat one in adsorbent surface
The layer good high molecular material of blood compatibility, had both improved the blood compatibility on absorbent resin surface, and can also reduce blood
Purifying adsorbent particles from getting loose enters the risk of blood.Be currently used to the modified high score material of coating mainly have nitrocellulose,
Poly hydroxy ethyl acrylate, cellulose acetate, modified polyvinylalcohol etc., these high molecular materials are in blood-purifying adsorbing agent tree
The coating on fat surface preferably resolves the unborn problem of blood-purifying adsorbing agent resin, clinically has and largely answer
With.
It is used for the high molecular material of coating above, the effect being modified to adsorbent surface is limited, in order to ensure adsorbent
Blood compatibility is realized often through coating thickness is increased, and is plugged the hole that suction-operated is played in part, is led to the suction of adsorbent
Attached effect with for compared with before modified, there is decline by a relatively large margin.Since existing its surface of membrane wrapping modified material is with a large amount of
Hydroxyl be also easy to cause complement activation phenomenon simultaneously although improving the blood compatibility of adsorbent to a certain extent
Generation, cause the adverse reactions such as the blood coagulation during blood purification treatment.The above both sides defect, restricts Blood index
The validity of product and safety.
Invention content
It is an object of the invention to overcome the prior art, provide a kind of for improving the life of blood purification material surface
The coating method of object compatibility.
The purpose of the present invention is achieved through the following technical solutions：One kind is for improving blood purification material surface biofacies
The coating method of capacitive, includes the following steps：
S1, adsorbing agent carrier processing：According to the target substance of absorption, the pore-size distribution that selects is 3~30nm, particle diameter distribution
It is 800~1200m for 0.3~1.2mm, specific surface area20.6~0.9cm of/g, Kong Rongwei3The porous adsorbent of/g, goes forward side by side and practises medicine
With grade purified treatment；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：It is poly- that dried sulfonation is added into n,N-Dimethylformamide solvent first
Sulfone, in 60 DEG C of stirring in water bath, until sulfonated polysulfone is completed to dissolve, and a concentration of the 0.5~2% of sulfonated polysulfone, it then adds anhydrous
Ethyl alcohol stirs and evenly mixs, and the volume ratio of the absolute ethyl alcohol and n,N-Dimethylformamide solvent is 0.05~0.2:1；
S22, coating：Absorbent resin is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 5~10min,
Extra coating liquid is filtered, under the state that vacuumizes, 50~60 DEG C of dry 2h, final rinse water absorbent resin is washed off residual
The n,N-Dimethylformamide and absolute ethyl alcohol stayed, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：The adsorbent of adsorbing agent carrier and existing other materials coating after coating is carried
Body is compared, its validity and safety are evaluated.
The sulfonation degree of the sulfonated polysulfone is 5~20%.
In step S1, the target substance of the absorption is the middle macromolecular toxins in uremic patient blood, the suction of selection
Attached dose of resin pore-size distribution is 3~15nm, particle diameter distribution is 0.6~1.2mm, specific surface area is 800~1200m2/ g, Kong Rongwei
0.6~0.8cm3The porous adsorbent of/g, and carry out medical grade purified treatment.
In step S1, the target substance of the absorption is the raised cell factor of exception in acute inflammation blood samples of patients,
The absorbent resin pore-size distribution selected is 5~30nm, particle diameter distribution is 0.3~1.2mm, specific surface area is 800~1200m2/
G, 0.7~0.9cm of Kong Rongwei3The porous adsorbent of/g, and carry out medical grade purified treatment.
In step S1, adsorbing agent carrier is macroporous absorbent resin, activated carbon or the carbide resin of synthesis.
The present invention has the following advantages：
1, the present invention uses sulfonated polysulfone material, surface coating modified to blood-purifying adsorbing agent progress, can change well
The blood compatibility of kind blood-purifying adsorbing agent resin reduces the dosage of product anti-coagulants in practical applications, reduces blood coagulation, molten
The probability of the generation of the adverse reactions such as blood, complement activation.
2, surface coating modified blood-purifying adsorbing agent is carried out using the present invention, because sulfonated polysulfone has good blood
Liquid phase capacitive can effectively improve the blood compatibility of adsorbent surface, reduce absorption of the absorption to plasma protein, blood platelet,
The probability that the adverse reactions such as blood coagulation, haemolysis, complement activation occur is reduced, makes product that there is good safety.
3, surface coating modified blood-purifying adsorbing agent is carried out using the present invention, because of the good blood phase of sulfonated polysulfone
Capacitive, it is only necessary to seldom amount is coated on blood-purifying adsorbing agent surface, can effectively improve the blood compatibility of adsorbent,
The pore structure of its left and right of adsorbent can be kept not to be blocked well, make product that there is excellent absorption property.
Specific implementation mode
With reference to embodiment, the present invention will be further described, but protection scope of the present invention is not limited to following institute
It states.
【Embodiment 1】
A kind of coating method for improving blood purification material surface biocompatibility includes the following steps：
S1, adsorbing agent carrier processing：The target substance of absorption is the middle macromolecular toxins in uremic patient blood, selection
Pore-size distribution be 3nm, particle diameter distribution 0.6mm, specific surface area 1200m2/ g, Kong Rongwei 0.6cm3The porous adsorbent of/g,
And medical grade purified treatment is carried out, adsorbing agent carrier is macroporous absorbent resin, activated carbon or the carbide resin of synthesis；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：It is poly- that dried sulfonation is added into n,N-Dimethylformamide solvent first
Sulfone, the sulfonation degree of the sulfonated polysulfone are 20%, in 60 DEG C of stirring in water bath, until sulfonated polysulfone completes dissolving, sulfonated polysulfone it is dense
Degree is 2%, then adds absolute ethyl alcohol, stirs and evenly mixs, the volume of the absolute ethyl alcohol and n,N-Dimethylformamide solvent
Than being 0.2:1；
S22, coating：Absorbent resin is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 10min, filter
Fall extra coating liquid, under the state that vacuumizes, 60 DEG C of dry 2h, final rinse water absorbent resin washes off remaining N,
Dinethylformamide and absolute ethyl alcohol, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：The adsorbent of adsorbing agent carrier and existing other materials coating after coating is carried
Body is compared, its validity and safety are evaluated, the specific steps are：After adsorbing agent carrier after coating is sterilized,
It is impregnated with physiological saline, then evaluates it to β2-microglobulin, parathormone, leptin, leucocyte with uremic patient blood plasma
The adsorption capacity of interleukin -6.Platelet adhesion rate, haemolysis, the coagulant property of adsorbent are evaluated according to standard GB/T16886.
【Embodiment 2】
A kind of coating method for improving blood purification material surface biocompatibility includes the following steps：
S1, adsorbing agent carrier processing：The target substance of absorption is the middle macromolecular toxins in uremic patient blood, selection
Pore-size distribution be 9nm, particle diameter distribution 0.9mm, specific surface area 1000m2/ g, Kong Rongwei 0.7cm3The porous adsorbent of/g,
And medical grade purified treatment is carried out, adsorbing agent carrier is macroporous absorbent resin, activated carbon or the carbide resin of synthesis；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：It is poly- that dried sulfonation is added into n,N-Dimethylformamide solvent first
Sulfone, the sulfonation degree of the sulfonated polysulfone are 10%, in 60 DEG C of stirring in water bath, until sulfonated polysulfone completes dissolving, sulfonated polysulfone it is dense
Degree is 1.2%, then adds absolute ethyl alcohol, stirs and evenly mixs, the body of the absolute ethyl alcohol and n,N-Dimethylformamide solvent
Product is than being 0.12:1；
S22, coating：Absorbent resin is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 8min, filters
Extra coating liquid, under the state that vacuumizes, 55 DEG C of dry 2h, final rinse water absorbent resin washes off remaining N, N-
Dimethylformamide and absolute ethyl alcohol, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：The adsorbent of adsorbing agent carrier and existing other materials coating after coating is carried
Body is compared, its validity and safety are evaluated, the specific steps are：After adsorbing agent carrier after coating is sterilized,
It is impregnated with physiological saline, then evaluates it to β2-microglobulin, parathormone, leptin, leucocyte with uremic patient blood plasma
The adsorption capacity of interleukin -6.Platelet adhesion rate, haemolysis, the coagulant property of adsorbent are evaluated according to standard GB/T16886..
【Embodiment 3】
A kind of coating method for improving blood purification material surface biocompatibility includes the following steps：
S1, adsorbing agent carrier processing：The target substance of absorption is the middle macromolecular toxins in uremic patient blood, selection
Pore-size distribution be 15nm, particle diameter distribution 1.2mm, specific surface area 800m2/ g, Kong Rongwei 0.8cm3The porous adsorbent of/g,
And medical grade purified treatment is carried out, adsorbing agent carrier is macroporous absorbent resin, activated carbon or the carbide resin of synthesis；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：It is poly- that dried sulfonation is added into n,N-Dimethylformamide solvent first
The sulfonation degree of sulfone, the sulfonated polysulfone is 5%, in 60 DEG C of stirring in water bath, until sulfonated polysulfone completes dissolving, the concentration of sulfonated polysulfone
It is 0.5%, then adds absolute ethyl alcohol, stirs and evenly mixs, the volume of the absolute ethyl alcohol and n,N-Dimethylformamide solvent
Than being 0.05:1；
S22, coating：Absorbent resin is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 5min, filters
Extra coating liquid, under the state that vacuumizes, 50 DEG C of dry 2h, final rinse water absorbent resin washes off remaining N, N-
Dimethylformamide and absolute ethyl alcohol, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：The adsorbent of adsorbing agent carrier and existing other materials coating after coating is carried
Body is compared, its validity and safety are evaluated, the specific steps are：After adsorbing agent carrier after coating is sterilized,
It is impregnated with physiological saline, then evaluates it to β2-microglobulin, parathormone, leptin, leucocyte with uremic patient blood plasma
The adsorption capacity of interleukin -6.Platelet adhesion rate, haemolysis, the coagulant property of adsorbent are evaluated according to standard GB/T16886, are such as schemed
Shown in Tables 1 and 2：
Table 1：Adsorption performance data applied to the three embodiment adsorbents in uremia field
Table 2：Applied to three, uremia field embodiment adsorbent blood compatibility evaluation result
It is obtained from table 1, according to the adsorbent applied to uremic patient prepared by the present invention, to macromolecular in uremia
There is toxin good absorption property to have a clear superiority compared with the product in clinical application, and the front and back absorption of coating
The absorption property variation of agent is small, during illustrating coating, can ensure that absorbent resin pore structure is not blocked up by coating substance well
Plug.
It is obtained from table 2, according to the adsorbent applied to uremic patient for preparing of the present invention, platelet adhesion rate,
Hemolysis, whole blood clotting time are had excellent performance, and compared with the product in Clinical practice, are had in terms of platelet adhesion reaction and haemolysis
It has a clear superiority, same level is in terms of whole blood clotting time.
【Embodiment 4】
A kind of coating method for improving blood purification material surface biocompatibility includes the following steps：
S1, adsorbing agent carrier processing：The target substance of absorption is the raised cell of exception in acute inflammation blood samples of patients
The factor, the pore-size distribution selected is 5nm, particle diameter distribution 0.3mm, specific surface area 1200m2/ g, Kong Rongwei 0.7cm3/ g's is more
Hole adsorbent, and medical grade purified treatment is carried out, adsorbing agent carrier is the macroporous absorbent resin, activated carbon or carbonization tree of synthesis
Fat；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：It is poly- that dried sulfonation is added into n,N-Dimethylformamide solvent first
Sulfone, the sulfonation degree of the sulfonated polysulfone are 20%, in 60 DEG C of stirring in water bath, until sulfonated polysulfone completes dissolving, sulfonated polysulfone it is dense
Degree is 2%, then adds absolute ethyl alcohol, stirs and evenly mixs, the volume of the absolute ethyl alcohol and n,N-Dimethylformamide solvent
Than being 0.2:1；
S22, coating：Absorbent resin is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 10min, filter
Fall extra coating liquid, under the state that vacuumizes, 60 DEG C of dry 2h, final rinse water absorbent resin washes off remaining N,
Dinethylformamide and absolute ethyl alcohol, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：The adsorbent of adsorbing agent carrier and existing other materials coating after coating is carried
Body is compared, its validity and safety are evaluated, the specific steps are：After adsorbing agent carrier after coating is sterilized,
It is impregnated with physiological saline, then evaluates it to tumor necrosis factor α, interleukin 8, interleukin-1 beta, white with to blood plasma
The adsorption capacity of cytokine -6, according to the platelet adhesion rate of the adsorbent after standard GB/T16886 evaluation coating, haemolysis,
Coagulant property.
【Embodiment 5】
A kind of coating method for improving blood purification material surface biocompatibility includes the following steps：
S1, adsorbing agent carrier processing：The target substance of absorption is the raised cell of exception in acute inflammation blood samples of patients
The factor, the pore-size distribution selected is 17nm, particle diameter distribution 0.8mm, specific surface area 1000m2/ g, Kong Rongwei 0.8cm3/ g's
Porous adsorbent, and medical grade purified treatment is carried out, adsorbing agent carrier is the macroporous absorbent resin, activated carbon or carbonization tree of synthesis
Fat；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：It is poly- that dried sulfonation is added into n,N-Dimethylformamide solvent first
Sulfone, the sulfonation degree of the sulfonated polysulfone are 10%, in 60 DEG C of stirring in water bath, until sulfonated polysulfone completes dissolving, sulfonated polysulfone it is dense
Degree is 1.3%, then adds absolute ethyl alcohol, stirs and evenly mixs, the body of the absolute ethyl alcohol and n,N-Dimethylformamide solvent
Product is than being 0.13:1；
S22, coating：Absorbent resin is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 7min, filters
Extra coating liquid, under the state that vacuumizes, 55 DEG C of dry 2h, final rinse water absorbent resin washes off remaining N, N-
Dimethylformamide and absolute ethyl alcohol, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：The adsorbent of adsorbing agent carrier and existing other materials coating after coating is carried
Body is compared, its validity and safety are evaluated, the specific steps are：After adsorbing agent carrier after coating is sterilized,
It is impregnated with physiological saline, then evaluates it to tumor necrosis factor α, interleukin 8, interleukin-1 beta, white with to blood plasma
The adsorption capacity of cytokine -6, according to the platelet adhesion rate of the adsorbent after standard GB/T16886 evaluation coating, haemolysis,
Coagulant property.
【Embodiment 6】
A kind of coating method for improving blood purification material surface biocompatibility includes the following steps：
S1, adsorbing agent carrier processing：The target substance of absorption is the raised cell of exception in acute inflammation blood samples of patients
The factor, the pore-size distribution selected is 30nm, particle diameter distribution 1.2mm, specific surface area 800m2/ g, Kong Rongwei 0.9cm3/ g's is more
Hole adsorbent, and medical grade purified treatment is carried out, adsorbing agent carrier is the macroporous absorbent resin, activated carbon or carbonization tree of synthesis
Fat；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：It is poly- that dried sulfonation is added into n,N-Dimethylformamide solvent first
The sulfonation degree of sulfone, the sulfonated polysulfone is 5%, in 60 DEG C of stirring in water bath, until sulfonated polysulfone completes dissolving, the concentration of sulfonated polysulfone
It is 0.5%, then adds absolute ethyl alcohol, stirs and evenly mixs, the volume of the absolute ethyl alcohol and n,N-Dimethylformamide solvent
Than being 0.05:1；
S22, coating：Absorbent resin is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 5min, filters
Extra coating liquid, under the state that vacuumizes, 50 DEG C of dry 2h, final rinse water absorbent resin washes off remaining N, N-
Dimethylformamide and absolute ethyl alcohol, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：The adsorbent of adsorbing agent carrier and existing other materials coating after coating is carried
Body is compared, its validity and safety are evaluated, the specific steps are：After adsorbing agent carrier after coating is sterilized,
It is impregnated with physiological saline, then evaluates it to tumor necrosis factor α, interleukin 8, interleukin-1 beta, white with to blood plasma
The adsorption capacity of cytokine -6.According to the platelet adhesion rate of the adsorbent after standard GB/T16886 evaluation coating, haemolysis,
Coagulant property, as shown in Table 3 and Table 4：
Table 3：Adsorption performance data applied to the three embodiment adsorbents in critical illness field
Table 4：Blood compatibility evaluation result applied to three, critical illness field embodiment adsorbent
The adsorbent applied to critical illness field prepared according to the present invention is can be seen that from the data in table 3, to inflammation
The adsorption capacity of the factor is good, and same level is in the product in clinical application.The adsorption capacity of absorbent resin after coating
Decline only by a small margin.
It can be obtained from the data in table 4, according to the adsorbent applied to critical illness field prepared by the present invention, blood phase
Capacitive amount is good, early platelet adhesion reaction, solution, on the clotting time, better than at this stage in the product of clinical application.
Claims (4)
1. a kind of coating method for improving blood purification material surface biocompatibility, it is characterised in that：Including following step
Suddenly：
S1, adsorbing agent carrier processing：According to the target substance of absorption, the pore-size distribution that selects is 3~30nm, particle diameter distribution is
0.3~1.2mm, specific surface area are 800~1200m20.6~0.9cm of/g, Kong Rongwei3The porous adsorbent of/g, use of practising medicine of going forward side by side
Grade purified treatment；
S2, adsorbing agent carrier coating：Including following sub-step：
S21, configuration sulfonated polysulfone coating liquid：Be added first into n,N-Dimethylformamide solvent dried sulphidity be 5 ~
20% sulfonated polysulfone, in 60 DEG C of stirring in water bath, until sulfonated polysulfone is completed to dissolve, a concentration of the 0.5~2% of sulfonated polysulfone, then
Absolute ethyl alcohol is added, is stirred and evenly mixed, the volume ratio of the absolute ethyl alcohol and n,N-Dimethylformamide solvent is 0.05~
0.2:1；
S22, coating：Porous adsorbent is added into the sulfonated polysulfone coating liquid configured, after being dispersed with stirring 5~10min, filters
Extra coating liquid, under the state that vacuumizes, 50~60 DEG C of dry 2h, final rinse water porous adsorbent is washed off remaining
N,N-Dimethylformamide and absolute ethyl alcohol, until n,N-Dimethylformamide and the residual of absolute ethyl alcohol meet the requirements；
S3, performance of the adsorbent evaluation：By the adsorbing agent carrier of adsorbing agent carrier and existing other materials coating after coating into
Row comparison, evaluates its validity and safety.
2. a kind of coating method for improving blood purification material surface biocompatibility according to claim 1, special
Sign is：In step S1, the target substance of the absorption is the middle macromolecular toxins in uremic patient blood, the aperture of selection
It is distributed as 3~15nm, particle diameter distribution is 0.6~1.2mm, specific surface area is 800~1200m20.6~0.8cm of/g, Kong Rongwei3/g
Porous adsorbent, by series medical grade purified treatment.
3. a kind of coating method for improving blood purification material surface biocompatibility according to claim 1, special
Sign is：In step S1, the target substance of the absorption is the raised cell factor of exception in acute inflammation blood samples of patients, choosing
The pore-size distribution selected is 5~30nm, particle diameter distribution is 0.3~1.2mm, specific surface area is 800~1200m2/ g, Kong Rongwei 0.7
~0.9cm3The porous adsorbent of/g, by the medical grade purified treatment of series.
4. a kind of coating method for improving blood purification material surface biocompatibility according to claim 1, special
Sign is：In step S1, porous adsorbent is macroporous absorbent resin, activated carbon or the carbide resin of synthesis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610705333.3A CN106238023B (en) | 2016-08-22 | 2016-08-22 | A kind of coating method for improving blood purification material surface biocompatibility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610705333.3A CN106238023B (en) | 2016-08-22 | 2016-08-22 | A kind of coating method for improving blood purification material surface biocompatibility |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106238023A CN106238023A (en) | 2016-12-21 |
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