CN106238023A - A kind of coating method for improving blood purification material surface biocompatibility - Google Patents
A kind of coating method for improving blood purification material surface biocompatibility Download PDFInfo
- Publication number
- CN106238023A CN106238023A CN201610705333.3A CN201610705333A CN106238023A CN 106238023 A CN106238023 A CN 106238023A CN 201610705333 A CN201610705333 A CN 201610705333A CN 106238023 A CN106238023 A CN 106238023A
- Authority
- CN
- China
- Prior art keywords
- adsorbent
- spsf
- peplos
- adsorbing agent
- agent carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3268—Macromolecular compounds
- B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28054—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their surface properties or porosity
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
- B01J20/3206—Organic carriers, supports or substrates
- B01J20/3208—Polymeric carriers, supports or substrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- External Artificial Organs (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
The present invention relates to a kind of coating method for improving blood purification material surface biocompatibility, comprise the following steps: S1, adsorbing agent carrier process: according to the target substance of absorption, the pore-size distribution of selection is 3～30nm, particle diameter is distributed as 0.3～1.2mm, specific surface area is 800～1200m2/ g, pore volume are 0.6～0.9cm3The porous adsorbent of/g, through the medical grade purified treatment of series；S2, adsorbing agent carrier peplos: include following sub-step: S21, configuration SPSF coating liquid；S22, peplos；S3, performance of the adsorbent evaluation: evaluate its effectiveness and safety.It is an advantage of the current invention that: be coated with the SPSF with good blood compatibility at adsorbent surface, on the premise of not blocked adsorbing pore structure, can effectively improve the blood compatibility of adsorbent surface, reduce adsorbent to plasma protein, hematoblastic absorption, reduce the probability that the untoward reaction such as blood coagulation, haemolysis, complement activation occur, make product have good safety, and the absorption property that adsorbent is good can be kept.
Description
Technical field
The present invention relates to a kind of coating method for improving blood purification material surface biocompatibility.
Background technology
The research of Blood index treatment disease starts from the forties in last century.Blood is inhaled by China from the phase at the beginning of the eighties in last century
Attached dose conducts in-depth research, and is widely used, especially in drug intoxication, liver failure, renal failure, autoimmune
The aspect such as disease, critical illness achieves fast development.Especially having obtained rapid development between last decade, range of application is increasingly
Extensively." blood purification standard operating procedure " that Ministry of Public Health is printed and distributed in February, 2010, the clear stipulaties adaptation of Blood index technology
Card scope, Blood index technology, as a kind of critical treatment pattern in blood purification technology, can be widely applied to maintenance saturating
Multiple fields such as analysis complication, severe liver diseases, acute poisoning, autoimmune disease, critical illness and hyperlipemia.
Now it is applied to the main active charcoal of adsorbent and the synthetic resin of Blood index, without surface-coating modification
Activated carbon or synthetic resin, when there are some researches show it with contacting blood, be easily caused blood coagulation, damage blood cells, particles from getting loose
Enter blood and cause thrombosis, limit the application in Blood index field of activated carbon and synthetic resin.
In order to solve sorbent material for Blood index time produced problem, scientists adsorbent surface coat one
The macromolecular material that layer blood compatibility is good, had both improved the blood compatibility on absorbent resin surface, and also can reduce blood
Purifying adsorbent particles from getting loose enters the risk of blood.Be currently used to the modified high score material of coating mainly have nitrocellulose,
Poly hydroxy ethyl acrylate, cellulose acetate, modified polyvinylalcohol etc., these macromolecular materials are in blood-purifying adsorbing agent tree
The coating on fat surface, preferably resolves the unborn problem of blood-purifying adsorbing agent resin, has substantial amounts of answering clinically
With.
More than being used for the macromolecular material of peplos, the effect modified to adsorbent surface is limited, in order to ensure adsorbent
Blood compatibility, realizes often through increasing coating thickness, plugs part and plays adsorbing hole, causes the suction of adsorbent
Attached effect, with for comparing before modified, has decline by a relatively large margin.Owing to existing its surface of membrane wrapping modified material is with in a large number
Hydroxyl, although improve the blood compatibility of adsorbent to a certain extent, but be also easily caused complement activation phenomenon simultaneously
Generation, cause the untoward reaction such as blood coagulation during blood purification treatment.Above both sides defect, governs Blood index
The effectiveness of product and safety.
Summary of the invention
It is an object of the invention to overcome the shortcoming of prior art, it is provided that one is used for improving blood purification material list and looks unfamiliar
The coating method of the thing compatibility.
The purpose of the present invention is achieved through the following technical solutions: one is used for improving blood purification material surface biofacies
The coating method of capacitive, comprises the following steps:
S1, adsorbing agent carrier process: according to the target substance of absorption, the pore-size distribution of selection is 3～30nm, particle diameter distribution
Be 0.3～1.2mm, specific surface area be 800～1200m2/ g, pore volume are 0.6～0.9cm3The porous adsorbent of/g, goes forward side by side and practises medicine
Use level purified treatment；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried sulfonation gather
Sulfone, 60 DEG C of stirring in water bath, completes to dissolve to SPSF, and the concentration of SPSF is 0.5～2%, then adds anhydrous
Ethanol, stirring and evenly mixing, the volume ratio of described dehydrated alcohol and DMF solvent is 0.05～0.2:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 5～10min,
Filter unnecessary coating liquid, under evacuation state, 50～60 DEG C of dry 2h, final rinse water absorbent resin, wash off residual
The DMF stayed and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbent of the adsorbing agent carrier after peplos with existing other materials peplos is carried
Body contrasts, and evaluates its effectiveness and safety.
The sulfonation degree of described SPSF is 5～20%.
In step S1, the target substance of described absorption is the middle macromolecular toxins in uremic patient blood, the suction of selection
Attached dose of resin pore-size distribution is 3～15nm, particle diameter is distributed as 0.6～1.2mm, specific surface area is 800～1200m2/ g, pore volume are
0.6～0.8cm3The porous adsorbent of/g, and carry out medical grade purified treatment.
In step S1, the target substance of described absorption is the cytokine that the exception in acute inflammation blood samples of patients raises,
The absorbent resin pore-size distribution selected is 5～30nm, particle diameter is distributed as 0.3～1.2mm, specific surface area is 800～1200m2/
G, pore volume are 0.7～0.9cm3The porous adsorbent of/g, and carry out medical grade purified treatment.
In step S1, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbide resin of synthesis.
The invention have the advantages that
1, the present invention uses SPSF material, carries out surface coating modified to blood-purifying adsorbing agent, can well change
The blood compatibility of kind blood-purifying adsorbing agent resin, reduces the consumption of product anticoagulant in actual applications, reduces blood coagulation, molten
The probability of the generation of the untoward reaction such as blood, complement activation.
2, the present invention is used to carry out surface coating modified blood-purifying adsorbing agent, because SPSF has good blood
Liquid phase capacitive, can effectively improve the blood compatibility of adsorbent surface, reduces and adsorbs plasma protein, hematoblastic absorption,
Reduce the probability that the untoward reaction such as blood coagulation, haemolysis, complement activation occur, make product have good safety.
3, the present invention is used to carry out surface coating modified blood-purifying adsorbing agent, because the blood phase that SPSF is good
Capacitive, it is only necessary in the amount that blood-purifying adsorbing agent surface-coated is little, just can effectively improve the blood compatibility of adsorbent,
Can well keep adsorbent pore structure around the most blocked, make product have the absorption property of excellence.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described, but protection scope of the present invention is not limited to following institute
State.
[embodiment 1]:
A kind of coating method for improving blood purification material surface biocompatibility, comprises the following steps:
S1, adsorbing agent carrier process: the target substance of absorption is the middle macromolecular toxins in uremic patient blood, select
Pore-size distribution be 3nm, particle diameter is distributed as 0.6mm, specific surface area is 1200m2/ g, pore volume are 0.6cm3The porous adsorbent of/g,
And carrying out medical grade purified treatment, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbide resin of synthesis；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried sulfonation gather
Sulfone, the sulfonation degree of described SPSF is 20%, 60 DEG C of stirring in water bath, completes to dissolve to SPSF, SPSF dense
Degree is 2%, then adds dehydrated alcohol, stirring and evenly mixing, described dehydrated alcohol and the volume of DMF solvent
Ratio is 0.2:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 10min, filter
Fall unnecessary coating liquid, under evacuation state, 60 DEG C of dry 2h, final rinse water absorbent resin, wash the N of residual off,
Dinethylformamide and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbent of the adsorbing agent carrier after peplos with existing other materials peplos is carried
Body contrasts, and evaluates its effectiveness and safety, and it concretely comprises the following steps: after the adsorbing agent carrier after peplos is carried out sterilizing,
Soak with normal saline, then evaluate it to B2M, parathyroid hormone, leptin, leukocyte with uremic patient blood plasma
The absorbability of interleukin-6.The platelet adhesion rate of adsorbent, haemolysis, coagulant property is evaluated according to standard GB/T16886.
[embodiment 2]:
A kind of coating method for improving blood purification material surface biocompatibility, comprises the following steps:
S1, adsorbing agent carrier process: the target substance of absorption is the middle macromolecular toxins in uremic patient blood, select
Pore-size distribution be 9nm, particle diameter is distributed as 0.9mm, specific surface area is 1000m2/ g, pore volume are 0.7cm3The porous adsorbent of/g,
And carrying out medical grade purified treatment, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbide resin of synthesis；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried sulfonation gather
Sulfone, the sulfonation degree of described SPSF is 10%, 60 DEG C of stirring in water bath, completes to dissolve to SPSF, SPSF dense
Degree is 1.2%, then adds dehydrated alcohol, stirring and evenly mixing, described dehydrated alcohol and the body of DMF solvent
Long-pending ratio is 0.12:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 8min, filter
Unnecessary coating liquid, under evacuation state, 55 DEG C of dry 2h, final rinse water absorbent resin, wash the N of residual, N-off
Dimethylformamide and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbent of the adsorbing agent carrier after peplos with existing other materials peplos is carried
Body contrasts, and evaluates its effectiveness and safety, and it concretely comprises the following steps: after the adsorbing agent carrier after peplos is carried out sterilizing,
Soak with normal saline, then evaluate it to B2M, parathyroid hormone, leptin, leukocyte with uremic patient blood plasma
The absorbability of interleukin-6.The platelet adhesion rate of adsorbent, haemolysis, coagulant property is evaluated according to standard GB/T16886..
[embodiment 3]:
A kind of coating method for improving blood purification material surface biocompatibility, comprises the following steps:
S1, adsorbing agent carrier process: the target substance of absorption is the middle macromolecular toxins in uremic patient blood, select
Pore-size distribution be 15nm, particle diameter is distributed as 1.2mm, specific surface area is 800m2/ g, pore volume are 0.8cm3The porous adsorbent of/g,
And carrying out medical grade purified treatment, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbide resin of synthesis；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried sulfonation gather
Sulfone, the sulfonation degree of described SPSF is 5%, 60 DEG C of stirring in water bath, completes to dissolve to SPSF, the concentration of SPSF
It is 0.5%, then adds dehydrated alcohol, stirring and evenly mixing, described dehydrated alcohol and the volume of DMF solvent
Ratio is 0.05:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 5min, filter
Unnecessary coating liquid, under evacuation state, 50 DEG C of dry 2h, final rinse water absorbent resin, wash the N of residual, N-off
Dimethylformamide and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbent of the adsorbing agent carrier after peplos with existing other materials peplos is carried
Body contrasts, and evaluates its effectiveness and safety, and it concretely comprises the following steps: after the adsorbing agent carrier after peplos is carried out sterilizing,
Soak with normal saline, then evaluate it to B2M, parathyroid hormone, leptin, leukocyte with uremic patient blood plasma
The absorbability of interleukin-6.The platelet adhesion rate of adsorbent, haemolysis, coagulant property is evaluated, such as figure according to standard GB/T16886
Shown in Tables 1 and 2:
Table 1: be applied to the adsorption performance data of three the embodiment adsorbents in uremia field
Table 2: be applied to three, uremia field embodiment adsorbent blood compatibility evaluation result
Drawing from table 1, the adsorbent being applied to uremic patient prepared according to the present invention, to macromole in uremia
Toxin has good absorption property, compares with product in clinical practice, adsorbs before and after having clear superiority, and peplos
The absorption property change of agent is little, during peplos is described, can well ensure that absorbent resin pore structure is not blocked up by coating substance
Plug.
Draw from table 2, the adsorbent being applied to uremic patient prepared according to the present invention, its platelet adhesion rate,
Hemolysis, whole blood clotting time excellent performance, compare with the product at Clinical practice, has in terms of platelet adhesion and haemolysis
Having a clear superiority in, whole blood clotting time aspect is in same level.
[embodiment 4]:
A kind of coating method for improving blood purification material surface biocompatibility, comprises the following steps:
S1, adsorbing agent carrier process: the target substance of absorption is the cell that the exception in acute inflammation blood samples of patients raises
The factor, the pore-size distribution of selection is 5nm, particle diameter is distributed as 0.3mm, specific surface area is 1200m2/ g, pore volume are 0.7cm3/ g is many
Hole adsorbent, and carry out medical grade purified treatment, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbonization tree of synthesis
Fat；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried sulfonation gather
Sulfone, the sulfonation degree of described SPSF is 20%, 60 DEG C of stirring in water bath, completes to dissolve to SPSF, SPSF dense
Degree is 2%, then adds dehydrated alcohol, stirring and evenly mixing, described dehydrated alcohol and the volume of DMF solvent
Ratio is 0.2:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 10min, filter
Fall unnecessary coating liquid, under evacuation state, 60 DEG C of dry 2h, final rinse water absorbent resin, wash the N of residual off,
Dinethylformamide and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbent of the adsorbing agent carrier after peplos with existing other materials peplos is carried
Body contrasts, and evaluates its effectiveness and safety, and it concretely comprises the following steps: after the adsorbing agent carrier after peplos is carried out sterilizing,
Soak with normal saline, then evaluate it to tumor necrosis factor α, interleukin 8, interleukin-1 beta, white with to blood plasma
The absorbability of cytokine-6, according to standard GB/T16886 evaluate the platelet adhesion rate of adsorbent after peplos, haemolysis,
Coagulant property.
[embodiment 5]:
A kind of coating method for improving blood purification material surface biocompatibility, comprises the following steps:
S1, adsorbing agent carrier process: the target substance of absorption is the cell that the exception in acute inflammation blood samples of patients raises
The factor, the pore-size distribution of selection is 17nm, particle diameter is distributed as 0.8mm, specific surface area is 1000m2/ g, pore volume are 0.8cm3/ g's
Porous adsorbent, and carry out medical grade purified treatment, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbonization tree of synthesis
Fat；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried sulfonation gather
Sulfone, the sulfonation degree of described SPSF is 10%, 60 DEG C of stirring in water bath, completes to dissolve to SPSF, SPSF dense
Degree is 1.3%, then adds dehydrated alcohol, stirring and evenly mixing, described dehydrated alcohol and the body of DMF solvent
Long-pending ratio is 0.13:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 7min, filter
Unnecessary coating liquid, under evacuation state, 55 DEG C of dry 2h, final rinse water absorbent resin, wash the N of residual, N-off
Dimethylformamide and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbent of the adsorbing agent carrier after peplos with existing other materials peplos is carried
Body contrasts, and evaluates its effectiveness and safety, and it concretely comprises the following steps: after the adsorbing agent carrier after peplos is carried out sterilizing,
Soak with normal saline, then evaluate it to tumor necrosis factor α, interleukin 8, interleukin-1 beta, white with to blood plasma
The absorbability of cytokine-6, according to standard GB/T16886 evaluate the platelet adhesion rate of adsorbent after peplos, haemolysis,
Coagulant property.
[embodiment 6]:
A kind of coating method for improving blood purification material surface biocompatibility, comprises the following steps:
S1, adsorbing agent carrier process: the target substance of absorption is the cell that the exception in acute inflammation blood samples of patients raises
The factor, the pore-size distribution of selection is 30nm, particle diameter is distributed as 1.2mm, specific surface area is 800m2/ g, pore volume are 0.9cm3/ g is many
Hole adsorbent, and carry out medical grade purified treatment, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbonization tree of synthesis
Fat；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried sulfonation gather
Sulfone, the sulfonation degree of described SPSF is 5%, 60 DEG C of stirring in water bath, completes to dissolve to SPSF, the concentration of SPSF
It is 0.5%, then adds dehydrated alcohol, stirring and evenly mixing, described dehydrated alcohol and the volume of DMF solvent
Ratio is 0.05:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 5min, filter
Unnecessary coating liquid, under evacuation state, 50 DEG C of dry 2h, final rinse water absorbent resin, wash the N of residual, N-off
Dimethylformamide and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbent of the adsorbing agent carrier after peplos with existing other materials peplos is carried
Body contrasts, and evaluates its effectiveness and safety, and it concretely comprises the following steps: after the adsorbing agent carrier after peplos is carried out sterilizing,
Soak with normal saline, then evaluate it to tumor necrosis factor α, interleukin 8, interleukin-1 beta, white with to blood plasma
The absorbability of cytokine-6.According to standard GB/T16886 evaluate the platelet adhesion rate of adsorbent after peplos, haemolysis,
Coagulant property, as shown in Table 3 and Table 4:
Table 3: be applied to the adsorption performance data of three the embodiment adsorbents in critical illness field
Table 4: be applied to the blood compatibility evaluation result of three the embodiment adsorbents in critical illness field
Data from table 3 are it can be seen that the adsorbent being applied to critical illness field prepared according to the present invention, to inflammation
The absorbability of the factor is good, is in same level with the product in clinical practice.The absorbability of absorbent resin after peplos
Decline the most by a small margin.
Data from table 4 can draw, the adsorbent being applied to critical illness field prepared according to the present invention, blood phase
Capacitive amount is good, and early on platelet adhesion, solution, clotting time, better than present stage is at the product of clinical practice.
Claims (5)
1. the coating method being used for improving blood purification material surface biocompatibility, it is characterised in that: include following step
Rapid:
S1, adsorbing agent carrier process: according to the target substance of absorption, the pore-size distribution of selection is 3～30nm, particle diameter is distributed as
0.3～1.2mm, specific surface area is 800～1200m2/ g, pore volume are 0.6～0.9cm3The porous adsorbent of/g, use of practising medicine of going forward side by side
Level purified treatment；
S2, adsorbing agent carrier peplos: include following sub-step:
S21, configuration SPSF coating liquid: in DMF solvent, first add dried SPSF,
60 DEG C of stirring in water bath, completing to dissolve to SPSF, the concentration of SPSF is 0.5～2%, then adds dehydrated alcohol,
Stirring and evenly mixing, the volume ratio of described dehydrated alcohol and DMF solvent is 0.05～0.2:1；
S22, peplos: in the SPSF coating liquid configured, add absorbent resin, after dispersed with stirring 5～10min, filter
Unnecessary coating liquid, under evacuation state, 50～60 DEG C of dry 2h, final rinse water absorbent resin, wash residual off
DMF and dehydrated alcohol, the residual to DMF and dehydrated alcohol meets the requirements；
S3, performance of the adsorbent evaluation: the adsorbing agent carrier of the adsorbing agent carrier after peplos with existing other materials peplos is entered
Row contrast, evaluates its effectiveness and safety.
A kind of coating method for improving blood purification material surface biocompatibility, it is special
Levy and be: the sulfonation degree of described SPSF is 5～20%.
A kind of coating method for improving blood purification material surface biocompatibility, it is special
Levying and be: in step S1, the target substance of described absorption is the middle macromolecular toxins in uremic patient blood, the aperture of selection
Be distributed as 3～15nm, particle diameter is distributed as 0.6～1.2mm, specific surface area is 800～1200m2/ g, pore volume are 0.6～0.8cm3/g
Porous adsorbent, through series medical grade purified treatment.
A kind of coating method for improving blood purification material surface biocompatibility, it is special
Levying and be: in step S1, the target substance of described absorption is the cytokine that the exception in acute inflammation blood samples of patients raises, choosing
The pore-size distribution selected is 5～30nm, particle diameter is distributed as 0.3～1.2mm, specific surface area is 800～1200m2/ g, pore volume are 0.7
～0.9cm3The porous adsorbent of/g, through the medical grade purified treatment of series.
A kind of coating method for improving blood purification material surface biocompatibility, it is special
Levying and be: in step S1, adsorbing agent carrier is macroporous adsorbent resin, activated carbon or the carbide resin of synthesis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610705333.3A CN106238023B (en) | 2016-08-22 | 2016-08-22 | A kind of coating method for improving blood purification material surface biocompatibility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610705333.3A CN106238023B (en) | 2016-08-22 | 2016-08-22 | A kind of coating method for improving blood purification material surface biocompatibility |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106238023A true CN106238023A (en) | 2016-12-21 |
| CN106238023B CN106238023B (en) | 2018-08-28 |
Family
ID=57595833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610705333.3A Active CN106238023B (en) | 2016-08-22 | 2016-08-22 | A kind of coating method for improving blood purification material surface biocompatibility |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106238023B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107649102A (en) * | 2017-09-30 | 2018-02-02 | 宁波市中心血站 | A kind of preparation method of compound doughnut polymeric adsorbent |
| CN108031454A (en) * | 2017-12-19 | 2018-05-15 | 陈荣胜 | Possesses blood-purifying adsorbing agent of physics specific selectivity and preparation method thereof |
| WO2018227448A1 (en) * | 2017-06-15 | 2018-12-20 | 财团法人祺华教育基金会 | Orally-administered poison adsorbent and manufacturing method therefor |
| CN111468079A (en) * | 2019-01-23 | 2020-07-31 | 重庆希尔康血液净化器材研发有限公司 | Preparation method of anticoagulant hemoperfusion adsorption material |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1505541A (en) * | 2001-04-18 | 2004-06-16 | ��ҽѧ��ʽ���� | Asymmetric porous films and process for producing the same |
| CN105289334A (en) * | 2015-11-25 | 2016-02-03 | 华东理工大学 | Composite forward osmosis membrane and preparation method thereof |
-
2016
- 2016-08-22 CN CN201610705333.3A patent/CN106238023B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1505541A (en) * | 2001-04-18 | 2004-06-16 | ��ҽѧ��ʽ���� | Asymmetric porous films and process for producing the same |
| CN105289334A (en) * | 2015-11-25 | 2016-02-03 | 华东理工大学 | Composite forward osmosis membrane and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 汤先觉等: "人工肝脏辅助装置吸附剂的研究硝酸纤维素柱状活性炭微囊(CAC)的制备与研究", 《重庆医科大学学报》 * |
| 程莉萍等: "血液净化膜材和抗凝剂对凝血过程中接触活化的影响", 《四川大学学报（医学版）》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018227448A1 (en) * | 2017-06-15 | 2018-12-20 | 财团法人祺华教育基金会 | Orally-administered poison adsorbent and manufacturing method therefor |
| CN107649102A (en) * | 2017-09-30 | 2018-02-02 | 宁波市中心血站 | A kind of preparation method of compound doughnut polymeric adsorbent |
| CN107649102B (en) * | 2017-09-30 | 2020-06-05 | 宁波市中心血站 | Preparation method of composite hollow fiber adsorption resin |
| CN108031454A (en) * | 2017-12-19 | 2018-05-15 | 陈荣胜 | Possesses blood-purifying adsorbing agent of physics specific selectivity and preparation method thereof |
| CN108031454B (en) * | 2017-12-19 | 2021-08-13 | 陈荣胜 | Blood purification adsorbent with physical specificity selectivity and preparation method thereof |
| CN111468079A (en) * | 2019-01-23 | 2020-07-31 | 重庆希尔康血液净化器材研发有限公司 | Preparation method of anticoagulant hemoperfusion adsorption material |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106238023B (en) | 2018-08-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106238023B (en) | A kind of coating method for improving blood purification material surface biocompatibility | |
| CN108031454B (en) | Blood purification adsorbent with physical specificity selectivity and preparation method thereof | |
| CN112871139B (en) | Whole blood perfusion adsorbent, preparation method and application thereof | |
| JP4578405B2 (en) | Adsorbent and adsorber for low density lipoprotein and fibrinogen capable of whole blood treatment | |
| CN104174386A (en) | Adsorbent for removing BETA-2 microglobulin in blood | |
| CN109692372B (en) | Five-layer blood perfusion device and blood perfusion method | |
| CN102361658B (en) | For removing the sorbent with protein binding material | |
| CN111500563B (en) | Method for purifying human coagulation factor VII from human plasma | |
| Harm et al. | Characterization of adsorbents for cytokine removal from blood in an in vitro model | |
| CN113426423B (en) | Adsorbent for removing LDL (low density lipoprotein) by extracorporeal blood circulation, preparation method thereof and perfusion device | |
| JP2004516888A (en) | How to detoxify carbohydrate-containing solutions | |
| WO2020151228A1 (en) | Method for preparing antibacterial hollow fiber membrane for blood purification | |
| CN107649102B (en) | Preparation method of composite hollow fiber adsorption resin | |
| CN109692371B (en) | Self-anticoagulation double-layer activated carbon blood perfusion device and blood perfusion method | |
| JP7312030B2 (en) | blood treatment beads | |
| CN203139198U (en) | Simple blood purifier | |
| CN112675820B (en) | Blood purification membrane and preparation method and application thereof | |
| RU2712630C1 (en) | Use of sorbent granules from super-cross-linked polystyrene "stirosorb 516" as a contact hemoactivator of blood cell elements | |
| CN108969818A (en) | The low-density lipoprotein absorber of blood plasma separation and plasma adsorption can be carried out simultaneously | |
| Gu et al. | Hemoperfusion | |
| Beena et al. | Chitosan: A novel matrix for hemoperfusion | |
| CN113372474B (en) | Blood perfusion resin and preparation method and application thereof | |
| WO2015136107A1 (en) | Adsorber granules for removing uraemic toxins | |
| Handa et al. | Correlation between bradykinin concentration and blood pressure during Rheocarna therapy: A single‐center case series | |
| CN104624170A (en) | Adsorbent for treating gram bacterial infection and blood perfusion device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |