CN106236323A - A kind of nerve trachea with contact guiding function and preparation method thereof and device - Google Patents
A kind of nerve trachea with contact guiding function and preparation method thereof and device Download PDFInfo
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- CN106236323A CN106236323A CN201610644758.8A CN201610644758A CN106236323A CN 106236323 A CN106236323 A CN 106236323A CN 201610644758 A CN201610644758 A CN 201610644758A CN 106236323 A CN106236323 A CN 106236323A
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- spray orifice
- nerve trachea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/24—Formation of filaments, threads, or the like with a hollow structure; Spinnerette packs therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
- A61F2240/002—Designing or making customized prostheses
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
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Abstract
The invention discloses a kind of nerve trachea with contact guiding function, including hollow membrane body, inwall has longitudinal groove structure；Invention additionally discloses a kind of preparation facilities, the hoistable platform including base, being arranged on base, two micro-injection pumps being arranged on hoistable platform end face and spinning head and be positioned at the coagulation basin immediately below spinning head；Spinning head includes: shower nozzle, and inside offers and wherein spray chamber that micro-injection pump is connected；Being positioned at the double-deck spray orifice of shower nozzle, outer layer spray orifice top connects with spray chamber, and internal layer spray orifice top another micro-injection pump external, bottom are extended outside outer layer spray orifice, and internal layer spray orifice outer wall has along the axially extended groove of internal layer spray orifice.Invention additionally discloses a kind of method preparing nerve trachea.The longitudinal groove structure on nerve trachea inwall that the present invention prepares concurrently forms with nerve trachea in preparation process, and planform is uniform and stable, it is to avoid the additional programs such as secondary operations.
Description
Technical field
The present invention relates to preparation method and the Preparation equipment of a kind of nerve trachea, be specifically related to one and there is contact guiding
Nerve trachea of function and preparation method thereof and device.
Background technology
Nervous system can be divided into maincenter and peripheral nervous system thereof, and its effect is regulation and controls being total to of each system in human body
With movable, body is made to become a complete entity.Along with the current production automation and traffic mechanization degree significantly
Improving, the incidence rate of peripheral nervous system lesions increases day by day.In daily life, various mechanical woundings, chemicals, locally
The reason such as ischemia and tumor all can cause peripheral nerve injury.The limbs disturbance caused due to peripheral nerve injury
The quality of life of patient will be had a strong impact on；Therefore, the always nerve of reparation, regeneration and the functional rehabilitation after peripheral nerve injury
Emphasis Deng related discipline research.In at present research and clinically application, for the less neurologic defect in gap (< 5mm), generally
Use microsurgical technique to sew up carrying out end-to-end adventitia at peripheral nerve detachment, for distance neurologic defect then
Neural transplantation need to be carried out.Directly sewing up and be confined to shorter neurotmesis, the most excessive stitching tension force is unfavorable for neuranagenesis；
Neural autotransplantation can cause losing for function of nervous system of district, and to neurologic defect supply source deficiency thicker relatively greatly, and allosome moves
Plant solution immune-suppression problems of still needing.
Along with the development of medical science, biology and tissue engineering, nerve trachea repairing nerve damage is used to become a kind of non-
Often there is the CO2 laser weld method of application prospect.Nerve trachea is a kind of for connecting neurotmesis gap, the inherent newborn god of protection
The tubular structure of warp.Nerve trachea method is less to the interference of neurotmesis end, and can isolate neural with surrounding tissue, reduces inflammation
Disease and neuromatous generation, provide the environment of a relative insulation for neuranagenesis.Meanwhile, regenerating nerve can in nerve trachea
Play the neural far-end chemotaxis to near-end, guide and promote newborn neural growth.Advantages based on these several respects, neural
Microcatheter technology is the restorative procedure of the peripheral nervous system lesions that Medical circle is greatly developed.At present, preparation is led for nerve
The material of pipe can be largely classified into two big classes: a class is natural macromolecular material, including collagen protein, gelatin, fibrin, shell
Polysaccharide etc., the advantage of this type of material is to have preferable biocompatibility, degradable, but mechanical performance is poor；Another kind of is artificial
Synthesis macromolecular material, including polylactic acid, polycaprolactone, polyvinyl alcohol etc., the advantage of this type of material is the controlled of material property
Property is higher.
Nerve trachea guides the regeneration of nerve synapse by the effect of brought into physical contact, mechanicalness restriction and longitudinal tension force
Direction, but the diameter of nerve trachea is generally about 1mm, is far longer than the characteristic dimension (1～10 μm) of neurocyte；Cause
This, nerve trachea be only nerve growth provide macroscopic view guiding, can not all sidedly analog neuron grow in vivo true
Microenvironment.Thus, the guiding theory of the present invention is: by Bionic Design, introduces connecing of micro-scale in single-chamber nerve trachea
Touch property Guiding factor, thus the neuranagenesis microenvironment such as analog cell epimatrix in conduit, prepare in hgher efficiency new one
For nerve trachea.
Summary of the invention
The present invention provides a kind of nerve trachea, has longitudinal groove structure inside nerve trachea, and longitudinal groove structure has
Contact guiding function.
A kind of nerve trachea with contact guiding function, including hollow membrane body, described hollow membrane body
There is on inwall longitudinal groove structure.
Preferably, the width of described longitudinal groove structure be 10～100 μm, the degree of depth be 0.05mm～0.15mm.
Add longitudinal groove structure at nerve trachea inwall, both can improve the contact area of pipe inner wall, can improve again
The space availability ratio of conduit cavity.The present invention prepares thin polymer film hollow pipe by dry/wet spinning silk method, and can be in the film
Blank pipe inwall forms the longitudinal groove structure that width is 10～100 μm, and longitudinal direction of the present invention refers to axial with nerve trachea
Parallel direction.
The present invention also provides for the preparation facilities of a kind of nerve trachea with contact guiding function, including base, installation
Hoistable platform on base, it is arranged on two micro-injection pumps and spinning heads of hoistable platform end face and is just positioned at spinning head
The coagulation basin of lower section；Described spinning head includes:
Shower nozzle, inside offers and wherein spray chamber that micro-injection pump is connected；
Being positioned at shower nozzle and the most sheathed double-deck spray orifice, outer layer spray orifice top connects with described spray chamber, internal layer spray orifice top
Hold another micro-injection pump external, bottom to extend outside outer layer spray orifice, internal layer spray orifice outer wall has along internal layer spray orifice axial
The groove extended.
Spinning head of the present invention be designed with inside and outside double-hole, wherein endoporus outer layer has longitudinal groove, can make conduit
The uniform micro-grooves of middle formation, the length direction of longitudinal groove is axially paralleled with internal layer spray orifice.
Preferably, described hoistable platform is provided with the fixing seat for installing spinning head, and described fixing seat is arbitrary adjacent
Both sides are provided with level indicator the most at an angle of 90.It is further preferred that be equipped with height adjustment bolt at the corner of described base.
By on base four altitude mixture control supported, the level of spinning head position being adjusted.
Preferably, the internal diameter of described internal layer spray orifice is 0.5～0.8mm, and the degree of depth of groove is 0.05～0.15mm.External diameter with
Spinning head number of grooves and gash depth change, typically in 0.9-1.0mm scope.Further preferably, the internal diameter of internal layer jet pipe is
0.7mm。
Preferably, the outer wall of described internal layer spray orifice is uniformly distributed 12～24 grooves.
Preferably, the shape of cross section of described groove is circular.Indent and the cylindrical groove structure that formed of evagination are adjacent
Two cross sections that indent is cylindrical and evagination is cylindrical are two tangent circles.
Further preferably, the center of circle of the cross section of described groove is not located on different radii and and internal layer according to gash depth
On the circle that spray orifice is concentric.
Further preferred, the present invention uses the number of grooves of with groove spinning head endoporus to have 12,16 and 24 respectively
Individual.Groove shape is circular, and indent and the circular of evagination form groove structure, and the internal diameter of internal layer jet pipe is 0.7mm.Wherein 12 groove
Interior pinhole design is: outer double wedge and inner groovy radius are 0.14mm, the outer double wedge center of circle be positioned at radius 0.843mm and with pin hole
On concentric circle, the inner groovy center of circle is positioned on radius 0.99mm and the concentric circle of pin hole.In 16 grooves, pinhole design is: outer double wedge
Being 0.12mm with inner groovy radius, the outer double wedge center of circle is positioned on radius 0.841mm and the circle concentric with pin hole, inner groovy circle
The heart is positioned on radius 0.96mm and the circle concentric with pin hole.In 12 grooves, pinhole design is: outer double wedge and inner groovy radius are
0.06mm, the outer double wedge center of circle is positioned on radius 0.9mm and the circle concentric with pin hole, and the inner groovy center of circle is positioned at radius 0.917mm
And on the circle concentric with interior pin hole.
Preferably, the spacing between outlet and the coagulation basin of described double-deck spray orifice is 1～3cm, more preferably 2cm.
Spinning head baseplane is referred to as the air gap height to the distance of the horizontal plane of coagulation basin.This is heavy for the air gap height
Wanting parameter to have a direct impact conduit solidifying and setting, need the air gap height to be adjusted when preparing conduit, electric up-down is put down
The upper and lower displacement of platform achieves above-mentioned regulatory function.
Further preferably, described shower nozzle is made up of upper and lower two parts, top half with centrally located vertical hole and
The transverse holes being positioned on sidewall, described vertical hole and transverse holes connect corresponding micro-injection pump by ready-packaged joint respectively；Under
The end face in half portion, with the described spray chamber of an indent, is connected by some communicating passage between spray chamber with described vertical through holes,
The bottom of spray chamber is with one section of described outer layer spray orifice being communicated to bottom surface, lower half；Pipeline one end connection horizontal stroke of one 90 ° bendings
Xiang Kong, the other end sequentially pass through spray chamber and outer layer spray orifice and extend outside outer layer spray orifice, and this pipeline runs through the part of outer layer spray orifice
For described internal layer spray orifice.
The degree of depth of coagulation basin is 1～2m.Further preferably the degree of depth of coagulation basin is at 1.5m, a diameter of 0.5m, is ensureing behaviour
While making space, air layer height both can be adjusted flexibly, it is also ensured that in spinning process, bottom spinning head and coagulation basin
There are enough distances allow nerve trachea stable formation, reduce the touching impact on spinning process at the bottom of the water surface and pond.
Preferably, described hoistable platform includes elevating mechanism and is arranged on the tabletop of platform at elevating mechanism top.
Further, described elevating mechanism includes that screw elevator, motor and connection motor output shaft rise with spiral
The reduction gearing of fall machine.Motor drives screw rod to rotate by reduction gearing, drives lifting platform by spiral lift mechanism.
Further, described tabletop of platform is made up of fixed plate and the installing plate of bonded to each other and fixing connection, described solid
Determining plate to fix with screw elevator top, described micro-injection pump and spinning head are arranged on installing plate end face.
Further, described hoistable platform is additionally provided with electric control box.
The lifting of platform realizes also by other existing modes existing.
The present invention also provides for a kind of method utilizing described preparation facilities to prepare nerve trachea, comprises the steps:
Polymer solution and coagulating agent are injected in outer layer spray orifice and internal layer spray orifice by corresponding micro-injection pump respectively, by
Double-deck spray orifice extrusion molding, enters to, in coagulation basin, treat behind the air gap between the outlet of spray orifice bilayer spray orifice and coagulation basin
After hollow membrane body dimensional stability, from coagulation basin, take out polymer nerve trachea sample；By resulting polymers nerve trachea
Sample deionized water wash is removed after excess of solvent and get final product.
The method of the present invention is a kind of one-shot forming to prepare inwall and have the polymer nerve trachea of longitudinal groove structure
Processing method.In this method, the longitudinal groove structure on inwall concurrently forms with nerve trachea in preparation process, and knot
Configuration shape is uniform and stable, it is to avoid the additional programs such as secondary operations.
Dry-wet spinning method is a kind of technique preparing hollow tubular film, and by polymer solution and non-solvent, (or other are molten
Liquid) inject in spinning head with micro-injection pump respectively, this biphase immiscible solution is through one section of air section or other control
Immerse in coagulation basin after atmosphere, solidify to form hollow tubular film.The technological parameter of spinning process is on nerve trachea and inwall
The geometric scale of groove has significant impact, therefore by the adjustment of technological parameter may be controlled to nerve trachea after shape
Geometric properties.Main spinning technology parameter includes: spinning head specification, and polymer solution rate of extrusion, non-solvent are extruded
Speed, polymer solution concentration and the air gap height etc..
Preferably, the rate of extrusion of described polymer solution is 0.8-2.6ml/min.
Rate of extrusion 0.8-2.8ml/min of coagulating agent (non-solvent or other solution).
Preferably, described polymer can be polyacrylonitrile, Poly(D,L-lactide-co-glycolide etc., dissolves the molten of polymer
Agent can be dimethyl sulfoxide etc.；Described coagulating agent can be water etc..
Concrete, comprise the steps:
(1) polymer solute is dissolved in solvent formation polymer solution, after standing the bubble removed in solution, is dried
Seal and preserve；
(2) polymer solution and non-solvent (i.e. coagulating agent) are respectively put in two syringes, the injection speed of syringe
Rate is controlled by two micro-injection pumps respectively；
(3) being placed on hoistable platform after spinning head dried, spinning head porch and syringe, with hose connection, are adjusted
Joint hoistable platform height is with the air gap height between regulation spinneret exits and coagulation basin horizontal plane, and enters processing platform
Row horizontal adjustment, makes spinning head outlet at bottom holding level；
(4) drive syringe that polymer solution and non-solvent are injected spinning head by micro-injection pump, go out from spinning head
Extrude at Kou, after the section of the air gap, enter coagulation basin, when, after doughnut dimensional stability, obtaining stable gathering from coagulation basin
Compound nerve trachea sample；
(5) the nerve trachea sample of collection is soaked in deionized water or with deionized water rinsing, it is unnecessary to remove
After solvent, by microscope, the physical dimension of nerve trachea is measured.
Compared with prior art, there is advantages that
One-shot forming of the present invention is prepared inwall and is had the polymer nerve trachea of longitudinal groove structure, in this method, interior
Longitudinal groove structure on wall concurrently forms with nerve trachea in preparation process, and planform is uniform and stable, it is to avoid two
The additional programs such as secondary processing.
Accompanying drawing explanation
Fig. 1 is the front view of invention preparation facilities.
Fig. 2 is the top view of preparation facilities of the present invention.
Fig. 3 is the left view of preparation facilities of the present invention.
Fig. 4 is the structural scheme of mechanism of the mechanism of elevating mechanism.
Fig. 5 is the structural representation of spinning head of the present invention.
Fig. 6 is the split schematic diagram of spinning head of the present invention.
Fig. 7 is the sectional view of spinning head of the present invention.
Fig. 8 is the enlarged drawing of part A in Fig. 7.
Fig. 9 is the sectional view of 12 grooves of spinning head endoporus of the present invention.
Figure 10 is the sectional view of 16 grooves of spinning head endoporus of the present invention.
Figure 11 is the sectional view of 24 grooves of spinning head endoporus of the present invention.Figure 12 A～Figure 12 D is that embodiment 1 and 2 is prepared into
The sectional view of the nerve trachea arrived.
Shown in figure, reference is as follows:
1-base 2-height adjustment bolt 3-hoistable platform
4-elevating mechanism 5-tabletop of platform 6-electric control box
7-the first micro-injection pump 8-the second micro-injection pump 9-spinning head
10-level indicator 11-ready-packaged joint 12-flexible pipe
13-coagulation basin
The vertical hole of 91-shower nozzle first half 92-shower nozzle lower half 93-
94-communicating passage 95-transverse holes 96-spray chamber
97-internal layer spray orifice 98-outer layer spray orifice 99-groove
41-screw elevator 42-reduction gearing 43-motor
Detailed description of the invention
As shown in Fig. 1～11, the preparation facilities of a kind of nerve trachea with contact guiding function, including base 1, the end
Being provided with height adjustment bolt 2 at the corner of seat 1, base 2 is installed a hoistable platform 3, hoistable platform 3 includes being arranged on base
On elevating mechanism 4 and the platform plane 5 on fixing elevating mechanism top, the structure of elevating mechanism 4 as shown in figure 11, including spiral
Formula elevator 41, motor 43 and connection motor output shaft and the reduction gearing 42 of screw elevator screw rod, motor is fixed on the end
On seat, motor driving the lifting of hoistable platform, the up-down mode of hoistable platform itself can use prior art to realize, platform platform
Being additionally provided with electric control box 6 on face, motor, the first micro-injection pump and the second micro-injection pump all access this electric control box.
Tabletop of platform 5 includes fixed plate and installing plate, fixed plate and the screw elevator of bonded to each other and fixing connection
Top is fixed, and installing plate is installed the first micro-injection pump the 7, second micro-injection pump 8 and spinning head 9, and spinning head 9 is by fixing
Seat is installed, and the fixing arbitrary adjacent both sides of seat arrange the level indicator 10 of mutual angle in 90 °.
Below platform plane and be positioned at immediately below spinning head coagulation basin 13 is set, the degree of depth of coagulation basin 13 be the degree of depth be 1～
2m, in present embodiment, the degree of depth is 1.5m, a diameter of 0.5m, and the vertical dimension between spinneret exits is about 2cm.
The main improvement of the present invention be spinning head structure improve, the structure of spinning head 9 as shown in Fig. 5～Figure 11,
Being formed (the shower nozzle first half 91 and shower nozzle lower half 92) by two parts, overall structure and Split type structure are as shown in Figure 5 and Figure 6.
The sectional view of spinning head as shown in Figure 7 and Figure 8, offers a vertical hole 93 and a transverse holes in the shower nozzle first half
95, vertical hole is positioned at the center of the shower nozzle first half, and transverse holes is positioned on the sidewall of the shower nozzle first half, two ready-packaged joints 11 points
Not threadeding with vertical hole and transverse holes, two ready-packaged joints connect the micro-injection pump of correspondence respectively by flexible pipe 12.
Shower nozzle lower half 92 end face is communicated to spray with the spray chamber 96 of an indent, the bottom of spray chamber 96 by one end through hole
The lower section (via bottoms opening is positioned on bottom surface, lower half) of head lower half, this through hole is the outer layer spray orifice 98 of double-deck spray orifice, perpendicular
Being connected by equally distributed communicating passage 94 between through hole 93 with spray chamber 96, hard pipeline one end of one 90 ° bendings is even
Shower nozzle lower half is extended after sequentially passing through spray chamber and outer layer spray orifice in logical transverse holes 95, one end, runs through the part structure of outer layer spray orifice
Becoming the internal layer spray orifice 97 of double-deck spray orifice, the shower nozzle first half that 90 ° of bending parts of hard pipeline are corresponding is solid construction, uniformly divides
The communicating passage 94 of cloth is i.e. uniformly distributed around this solid construction part, is set to 4～6 communicating passage in present embodiment.
Having groove 99 on the outer wall of internal layer spray orifice 97, groove is longitudinal groove, and axially extending along internal layer spray orifice, it is transversal
Face figure is as shown in Fig. 9～11, and the shape of cross section of longitudinal groove is circular, indent and the circular groove structure that formed of evagination, vertical
It is positioned on different radii and the circle concentric with internal layer spray orifice according to gash depth to the cross section center of circle of groove.
Being uniformly distributed 12～24 grooves on the outer wall of internal layer spray orifice, the internal diameter of internal layer spray orifice is 0.5～0.8mm, external diameter
Changing with spinning head number of grooves and gash depth, the degree of depth of groove is 0.05mm～0.15mm.
The inner hole section figure of 12 grooves is as it is shown in figure 9, outer double wedge and inner groovy radius are 0.14mm, the outer double wedge center of circle
Being positioned on radius 0.843mm and the circle concentric with pin hole, the inner groovy center of circle is positioned at radius 0.99mm and the concentric circle of pin hole
On.
As shown in Figure 10, outer double wedge and inner groovy radius are 0.12mm, outer double wedge circle to the inner hole section figure of 16 grooves
The heart be positioned at radius 0.841mm and and the concentric circle of pin hole on, the inner groovy center of circle be positioned at radius 0.96mm and and pin hole concentric
Circle on.
As shown in figure 11, outer double wedge and inner groovy radius are 0.06mm, outer double wedge circle to the inner hole section figure of 24 grooves
The heart be positioned at radius 0.9mm and and the concentric circle of pin hole on, the inner groovy center of circle be positioned at radius 0.917mm and and pin hole concentric
On circle.
The method being prepared nerve trachea by this device is as follows:
(1) polymer solute is dissolved in solvent formation polymer solution, after standing the bubble removed in solution, is dried
Seal and preserve；
(2) polymer solution and non-solvent being respectively put in two syringes, the injection rate of syringe is respectively by two
Platform micro-injection pump controls；
(3) being placed on hoistable platform after spinning head dried, spinning head porch and syringe, with hose connection, are used
The air gap height between lifting platform controller regulation spinneret exits and coagulation basin horizontal plane, and processing platform is carried out
Horizontal adjustment, makes spinning head outlet at bottom holding level；
(4) drive syringe that polymer solution and non-solvent are injected spinning head by micro-injection pump, go out from spinning head
Extrude at Kou, after the section of the air gap, enter coagulation basin, when, after doughnut dimensional stability, obtaining stable gathering from coagulation basin
Compound nerve trachea sample；
(5) the nerve trachea sample of collection is soaked in deionized water or with deionized water rinsing, it is unnecessary to remove
After solvent, by microscope, the physical dimension of nerve trachea is measured.
The specific embodiment being prepared nerve trachea by apparatus of the present invention and method is as follows:
Embodiment 1, prepare polyacrylonitrile nerve trachea
In the present embodiment, polymeric material is polyacrylonitrile (PAN), and solvent material is dimethyl sulfoxide (DMSO), non-solvent
It is deionized water with coagulating agent material.Being dissolved in dimethyl sulfoxide by polyacrylonitrile, forming concentration is the poly-of 6-10w/w%
Polymer solution.Polymer solution stirs at normal temperatures 8 hours and enables polymer uniform dissolution, stands 12 hours and removes in solution
Bubble after, be dried seal preserve.Polyacrylonitrile solution and deionized water are respectively put in two sample introduction needle, in micro-injection
Under pump controls, the flow speed control scope of polymer solution and deionized water is 0.5 3ml/min.It is respectively adopted outer wall smooth interior
Hole spinning head and endoporus have the spinning head of 12,16 grooves and are prepared, and the air gap height is set to 2cm.Polymer solution
After spinning head is extruded, after the section of the air gap, enter coagulation basin with deionized water, obtain steady after flow field is stable from coagulation basin
Fixed polymer nerve trachea sample.Being soaked in by the nerve trachea sample of collection in deionized water 24 hours, it is unnecessary to remove
Solvent.Being observed by microscope, the cross section of gained hollow nerve trachea is as shown in Figure 12 A～Figure 12 C, and wherein Figure 12 A is inwall
Smooth polyacrylonitrile nerve trachea, Figure 12 B is the polyacrylonitrile nerve trachea having 12 grooves, and Figure 12 C is 16 grooves
Polyacrylonitrile nerve trachea.
Embodiment 2, prepare Poly(D,L-lactide-co-glycolide nerve trachea
In this example, polymeric material is Poly(D,L-lactide-co-glycolide (PLGA), and solvent material is dimethyl sulfoxide
(DMSO), non-solvent and coagulating agent material are deionized water.Poly(D,L-lactide-co-glycolide is dissolved in dimethyl sulfoxide
In, form the polymer solution that concentration is 15-25w/w%.Poly(D,L-lactide-co-glycolide dissolves relatively under the conditions of bulk
Slowly, ultrasound cleaner vibrator can be used to move a little time to accelerate dissolution velocity.Polymer solution stirs at normal temperatures 8 hours and makes
Polymer can uniform dissolution, stand after within 12 hours, removing bubble removing, be dried seal preserve.By Poly(D,L-lactide-co-glycolide
Solution and deionized water are respectively put in two sample introduction needle, under micro-injection pump control, polymer solution and deionized water
Flow speed control scope is 0.5 2ml/min.It is respectively adopted smooth inner bore spinning head and endoporus has the spinning head of 12,16 grooves
Being prepared, the air gap height is set to 2cm.Polymer solution and deionized water after spinning head is extruded through the air gap section
Rear entrance coagulation basin, obtains stable polymer nerve trachea sample after flow field is stable from coagulation basin.The nerve that will collect
Catheter samples deionized water is rinsed, and removes solvent unnecessary in conduit, and kept dry is to prevent poly lactic-co-glycolic acid
Copolymer is degraded in water.Being observed by microscope, gained inwall has cross section such as Figure 12 D of the PLGA nerve trachea of 12 grooves
Shown in.
The foregoing is only the case that is embodied as of patent of the present invention, but the technical characteristic of patent of the present invention is not limited to
This, any those skilled in the relevant art in the field of the invention, the change made or modify all contain in the present invention special
Among profit scope.
Claims (10)
1. there is a nerve trachea for contact guiding function, including hollow membrane body, it is characterised in that described hollow is thin
There is on the inwall of membrane tube body longitudinal groove structure.
Nerve trachea the most according to claim 1, it is characterised in that the width of described longitudinal groove structure be 50～300 μm,
The degree of depth is 0.05mm～0.15mm.
3. there is a preparation facilities for the nerve trachea of contact guiding function, including base, the lifting that is arranged on base
Platform, two micro-injection pumps being arranged on hoistable platform end face and a spinning head and be positioned at the condensation immediately below spinning head
Pond；It is characterized in that, described spinning head includes:
Shower nozzle, inside offers and wherein spray chamber that micro-injection pump is connected；
Being positioned at shower nozzle and the most sheathed double-deck spray orifice, outer layer spray orifice top connects with described spray chamber, bottom extends to shower nozzle
Bottom surface, internal layer spray orifice top another micro-injection pump external, bottom are extended outside outer layer spray orifice, and internal layer spray orifice outer wall has
Along the axially extended groove of internal layer spray orifice.
Preparation facilities the most according to claim 3, it is characterised in that described hoistable platform is provided with for installing spinning head
Fixing seat, the arbitrary adjacent both sides of described fixing seat are provided with level indicator the most at an angle of 90.
Preparation facilities the most according to claim 3, it is characterised in that the internal diameter of described internal layer spray orifice is 0.5～0.8mm, ditch
The degree of depth of groove is 0.05mm～0.15mm.
Preparation facilities the most according to claim 3, it is characterised in that be uniformly distributed 12～24 on the outer wall of described internal layer spray orifice
Individual groove.
Preparation facilities the most according to claim 3, it is characterised in that the cross section of described groove is circular.
Preparation facilities the most according to claim 7, it is characterised in that the center of circle of the cross section of described groove is according to gash depth
It is not located on different radii and the circle concentric with internal layer spray orifice.
Preparation facilities the most according to claim 7, it is characterised in that between outlet and the coagulation basin of described double-deck spray orifice
Away from for 1～3cm.
10. utilize the method that preparation facilities described in claim 3～9 any claim prepares nerve trachea, its feature
It is, comprises the steps:
Polymer solution and coagulating agent are injected in outer layer spray orifice and internal layer spray orifice by corresponding micro-injection pump, by bilayer respectively
Spray orifice extrusion molding, enters to, in coagulation basin, treat hollow membrane behind the air gap between double-deck spray orifice outlet and coagulation basin
After body dimensional stability, from coagulation basin, take out polymer nerve trachea sample；Resulting polymers nerve trachea sample is gone from
Sub-water washs after removing excess of solvent and get final product.
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| CN109620467A (en) * | 2018-11-28 | 2019-04-16 | 浙江大学 | A kind of nerve trachea formula Artificial neural and preparation method thereof |
| CN112853510A (en) * | 2019-11-28 | 2021-05-28 | 中国科学院大连化学物理研究所 | Controllable preparation method of inner groove microfilament based on microfluidic technology |
| CN114381812A (en) * | 2022-01-24 | 2022-04-22 | 中国科学院苏州纳米技术与纳米仿生研究所 | Spinning nozzle, nano material assembly and preparation method thereof |
| CN114870086A (en) * | 2022-05-23 | 2022-08-09 | 宁波市第一医院 | Artificial nerve conduit with PLGA and extracellular matrix removed as substrates, preparation method and device |
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| CN114870086A (en) * | 2022-05-23 | 2022-08-09 | 宁波市第一医院 | Artificial nerve conduit with PLGA and extracellular matrix removed as substrates, preparation method and device |
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